Active insecticide for preventing and treating carambola bactrocera dorsalis

Abstract

The invention belongs to the technical field of insecticides, and particularly relates to an active insecticide for preventing and treating carambola bactrocera dorsalis. The invention relates to an active insecticide, the active insecticide comprises an effective component and an auxiliary component, and the effective component is formed by compounding oxazole sulfonyl pyridine and tolfenpyrad or butene-fipronil; the mass ratio of the oxazole sulfonyl pyridine to the tolfenpyrad is (1-30): (20-1); the mass ratio of the oxazole sulfonyl pyridine to the butene-fipronil is (1-10): (10-1). According to the invention, a cotton ball feeding method is adopted to determine the combined action type of the oxazole sulfonyl pyridine and the tetratolfenpyrad or the butene-fipronil on the Carambola citrus fruit flies, and the oxazole sulfonyl pyridine and the tetratolfenpyrad or the butene-fipronil are found to have a synergistic effect on killing the Carambola citrus fruit flies under the condition of different mass ratios. Compared with a single component, the composition can improve the control effect on carambola bactrocera dorsalis, can reduce the pesticide dosage, reduce pesticide residues and relieve environmental pollution, and is beneficial to green, healthy and sustainable development of the carambola industry.

Description

Technical Field

[0001] The invention belongs to the technical field of insecticides, and particularly relates to an active insecticide for preventing and controlling carambola fruit flies. Background Art

[0002] Carambola belongs to the genus Pentatomus of the family Solanaceae. It can bloom and bear fruit many times a year and has high economic value. During the growth and development of carambola, it will be harmed by various pests such as the citrus fruit fly, bird feather moth, aphids and red spiders. Among them, the citrus fruit fly has a high reproductive capacity, with 8-9 generations a year. The pest occurs all year round, and has strong adaptability, no obvious overwintering phenomenon, and a wide host range. The citrus fruit fly lays eggs in mature fruits. After the fruit is damaged by the citrus fruit fly, insect holes are formed, making the fruit lose its sales value; at the same time, the larvae feed on the flesh after hatching, causing the fruit to rot and fall, resulting in a reduction in carambola production.

[0003] Chemical control is currently the main means of killing the citrus fruit fly, but in the process of agricultural disease control, disease resistance is an issue that cannot be ignored. Studies have shown that with the large-scale application of pesticides, the citrus fruit fly has developed resistance to different pesticides such as organophosphates, pyrethroids and avermectins, and environmental pollution and pesticide residues are becoming increasingly serious, seriously affecting the quality of fruits. Therefore, it is urgent to develop new safe and efficient pesticides.

[0004] Combining different insecticide active ingredients is a common method for preventing and controlling resistant diseases. When different active ingredients are combined, they usually show three types of combined effects: synergistic effect, additive effect and antagonistic effect. In the vast majority of cases, compound combinations show additive effects, and there are very few compound combinations that truly have synergistic effects, especially those with very obvious synergistic effects.

[0005] Oxazosulfyl is a new broad-spectrum benzothiophene insecticide containing ethylsulfonylpyridine structure fragment reported by Sumitomo Chemical Co., Ltd. of Japan. CAS No.: 1616678-32-0, molecular formula: C 15 H 11 F3N2O5S2, relative molecular mass: 420.383, structural formula is as follows:

[0006]

[0007] Oxazolidinone has a novel structure, and its molecular structure contains benzothiophene fragments and sulfonylpyridine fragments. Among them, the benzothiophene fragment is present in herbicides such as (fine) oxazolidinone and oxazolidinone, and the sulfonylpyridine fragment is widely present in sulfonylurea herbicides. The prior art has reported that oxazolidinone is active against Hemiptera, Lepidoptera, Diptera, Coleoptera and Orthoptera, and can be used to control various crop pests such as planthoppers, borers, flea beetles, borers, thrips, noctuids and fruit flies. However, as an insecticide, if a single ingredient is used for a long time, there is bound to be a high risk of drug resistance.

[0008] The inventors used the cotton ball feeding method to determine the type of combined action of oxazolidinone and existing insecticidal active ingredients on carambola fruit flies, and found that oxazolidinone and tetrazopyrid or butene fipronil showed a synergistic effect in killing carambola fruit flies at different mass ratios. No relevant combination reports have been seen so far. Summary of the invention

[0009] The object of the present invention is to provide an active insecticide for controlling carambola fruit fly, so as to solve the problem that the fruit fly has developed resistance to different insecticides.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] An active insecticide, comprising an active ingredient and an auxiliary ingredient, wherein the active ingredient is compounded by oxazolidinone and tetrazolin or butene-fipronil; the mass ratio of oxazolidinone to tetrazolin is 1-30:20-1; the mass ratio of oxazolidinone to butene-fipronil is 1-10:10-1.

[0012] Furthermore, the auxiliary ingredients are selected from one or more of dispersants, wetting agents, solvents, cosolvents, inert fillers, disintegrants, thickeners, film formers, penetrants, defoamers, antifreeze agents, preservatives, pH regulators, synergists and warning colors.

[0013] Furthermore, the mass of the active ingredient accounts for 5-55% of the total mass of the active pesticide.

[0014] The invention also provides application of the active insecticide in preventing and controlling carambola fruit fly.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The present invention uses a cotton ball feeding method to determine the combined action type of oxazolidinone and tetrazolyl thiocarbamide or butenyl fipronil on carambola fruit flies, and finds that oxazolidinone and tetrazolyl thiocarbamide or butenyl fipronil have a synergistic effect on killing carambola fruit flies at different mass ratios. Compared with a single component, the control effect on carambola fruit flies can be improved, the amount of pesticides used can be reduced, pesticide residues can be reduced, environmental pollution can be alleviated, and the green, healthy and sustainable development of the carambola industry can be contributed to.

[0017] (2) Compared with the single use of oxazolidinone, the active insecticide of the present invention can reduce the risk of drug resistance and delay the generation and development of pest resistance. DETAILED DESCRIPTION

[0018] The technical solution of the present invention is described clearly and completely below.

[0019] Example

[0020] 1. Test insect source

[0021] Bactrocera dorsalis was collected from carambola plantations and fed continuously for more than 10 generations in the laboratory to form a stable population. Two-day-old adults from the same batch were selected as the test insect source.

[0022] 2. Test reagents

[0023] 98% oxazolidinone technical (Shanghai Yuanxi Biotechnology Co., Ltd.), 90% tetrazobactam technical (Bayer AG), 99% butene fipronil technical (Hubei Xinkang Pharmaceutical Chemical Co., Ltd.)

[0024] 3. Test methods

[0025] The toxicity of oxazolidinone, tetrazobacteramide, butene-fipronil and their mixtures to the fruit fruit fly was determined by cotton ball feeding method.

[0026] The test agent was dissolved in methyl sulfoxide and then diluted with 10% honey solution (the solvent is 0.1% Tween-80 aqueous solution) to form a single-dose stock solution. The single dose was diluted with 10% honey solution (the solvent is 0.1% Tween-80 aqueous solution) in equal proportions into 7 mass concentration gradients, and then the toxicity of the single dose to the carambola fruit fly was determined.

[0027] On the basis of the single-dose toxicity determination, multiple groups of mixtures were set up, and each group of mixtures was diluted into 7 mass concentration gradients with 10% honey solution (the solvent was 0.1% Tween-80 aqueous solution) in equal proportions, and then the toxicity of each mixture to the carambola fruit fly was determined.

[0028] The specific method for the determination of the toxicity of single-dose and mixed-dose agents is as follows: soak the sterilized absorbent cotton balls in the test solution and take them out, put them into a 250mL triangular flask after the solution stops dripping, and inoculate 10 test adults (male-female ratio 1:1) that have been starved for 12 hours in each triangular flask, and set the treatment with 10% honey solution (solvent is 0.1% Tween-80 aqueous solution) as a blank control, and repeat each treatment 3 times. After inoculating the test adults, seal the triangular flask with a single layer of medical gauze and place it in an artificial climate box with a temperature of (25±1)℃, a relative humidity of 65±5%, and a photoperiod of 14L / 10D.

[0029] Observe the death of the test insects 48 hours after treatment, record the total number of insects and the number of dead insects in each treatment, and calculate the corrected mortality rate of each treatment. Perform linear regression with the logarithm of the drug concentration as x and the corrected mortality probability value as y to obtain the toxicity regression equation and the toxicity LC of the drug to the test pests. 50 value.

[0030]

[0031] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0032]

[0033] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0034] 4. Data Analysis

[0035] The co-toxicity coefficient (CTC) was calculated using the Sun Yunpei co-toxicity coefficient method, and the type of combined action of the compound combination on the test strain was evaluated based on the co-toxicity coefficient value: CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0036] Table 1 The results of toxicity test of oxazolidinone and tetrazobacteramide against Bactrocera dorsalis

[0037]

[0038] As shown in Table 1, within the mass ratio range of 1-30:20-1, the combination of oxazolidinone and tetrazobacteramide shows a synergistic effect in killing the fruit fly. This shows that the combination of oxazolidinone and tetrazobacteramide in the present invention can improve the control effect of the fruit fruit fly, reduce the amount of pesticides used, reduce pesticide residues, and alleviate environmental pollution.

[0039] Table 2 The results of toxicity test of oxazolidinone and butene fipronil complex to Bactrocera dorsalis

[0040]

[0041] As shown in Table 2, within the mass ratio range of 1-10:10-1, the combination of oxazolidinone and butene fipronil exhibits a synergistic effect on killing the fruit fly. This indicates that the combination of oxazolidinone and butene fipronil in the present invention can improve the control effect on the fruit fly, reduce the amount of pesticides used, reduce pesticide residues, and alleviate environmental pollution.

[0042] The present invention adopts a cotton ball feeding method to determine the combined action type of oxazolidinone and tetrazolyl thiocarbamide or butene fipronil on carambola fruit flies, and finds that oxazolidinone and tetrazolyl thiocarbamide or butene fipronil show a synergistic effect on killing carambola fruit flies under different mass ratios. Compared with a single component, the control effect on carambola fruit flies can be improved, the amount of pesticides used can be reduced, the pesticide residues can be reduced, the environmental pollution can be alleviated, and the green, healthy and sustainable development of the carambola industry can be contributed to.

Claims

1. An active insecticide, characterized in that The insecticide comprises an active ingredient and an auxiliary ingredient, wherein the active ingredient is a compound of oxazolidinone and tetrazopyrid or butene-fipronil; The mass ratio of oxazolidinone to tetrazolid is 1-30:20-1; The mass ratio of the oxazolidinone to the butene-fipronil is 1-10:10-1.

2. The active insecticide according to claim 1, characterized in that The auxiliary components are selected from one or more of dispersants, wetting agents, solvents, cosolvents, inert fillers, disintegrants, thickeners, film formers, penetrants, defoamers, antifreeze agents, preservatives, pH regulators, synergists and warning colors.

3. The active insecticide according to claim 1, characterized in that The mass of the active ingredient accounts for 5-55% of the total mass of the active insecticide.

4. Use of the active insecticide according to any one of claims 1 to 3 in controlling carambola fruit fly.

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