Application of siraitia grosvenorii extract as growth regulator

Abstract

The invention relates to an application of a momordica grosvenori extract as a growth regulator, belongs to the field of plant growth regulators, and aims to overcome the defects and defects in the prior art, the momordica grosvenori extract is used as the growth regulator to promote the growth of plants. The plant growth promoter is used for increasing the yield of plants, and / or improving the survival rate of the plants, and / or improving the quality of fresh fruits and / or prolonging the preservation time of the fresh fruits, and has the beneficial effects that the plant growth is promoted, the yield is increased, and the plant growth promoter is friendly to human bodies, environment and plants and has no harm.

Description

Technical Field

[0001] The invention belongs to the field of plant growth regulators, and in particular relates to the application of monk fruit extract as a growth regulator in increasing plant yield and improving plant quality. Background Art

[0002] Traditional plant growth regulators are generally divided into: hormone plant growth regulators, including auxin, cytokinin, cell differentiation hormone, cell expansion hormone, cell nutrients, etc., which can regulate plant growth and development; enzyme inhibitor plant growth regulators, including phosphatase inhibitors, deoxyribonuclease inhibitors, protease inhibitors, etc., which can regulate plant growth and immunity; polypeptide plant growth regulators, including extracellular polypeptides, intracellular polypeptides, etc., which can regulate plant hormone synthesis, cell division, cell expansion, cell differentiation and other growth and development processes. The use of plant growth regulators plays an important role in agricultural production, but the above traditional plant growth regulators also have some disadvantages and shortcomings during use: 1. Imprecise regulation of plant growth: The use of plant growth regulators requires precise control of concentration and application time, otherwise it may cause abnormal plant growth. For example, the use of too much auxin will cause excessive plant growth, while the use of excessive inhibitors may cause plant growth stagnation. 2. Pollution of soil and environment: Plant growth regulators may pollute groundwater, rivers, lakes and other water bodies through rainwater runoff, soil infiltration, etc., and affect aquatic ecosystems. In addition, long-term use of plant growth regulators may cause changes in the microbial community in the soil, leading to damage to the soil structure and decreased fertility. 3. Potential threats to human health: Plant growth regulators may pose a potential threat to human health. For example, some plant growth regulators have anti-nutritional effects that reduce the quality and nutritional value of plants. In addition, some plant growth regulators may remain in food, posing a potential threat to human health. 4. Produce phytotoxicity: If plant growth regulators are used improperly or excessively, they may cause phytotoxicity to plants. For example, using too much inhibitors may cause plant leaves to shrink, fruits to be deformed, etc. 5. High cost: The production and use costs of plant growth regulators are generally high, which will increase the cost of agricultural production.

[0003] In addition, some plant growth regulators are relatively expensive, which may limit their application in agricultural production. Summary of the invention

[0004] In view of the above-mentioned drawbacks and shortcomings in the prior art, the present invention provides a plant growth regulator with a wide concentration control range, no pollution to the soil and the environment, no phytotoxicity and relatively low cost, specifically an application of a monk fruit extract as a plant growth regulator in increasing plant yield and improving plant quality.

[0005] The main purpose of the present invention is to provide a new use of the monk fruit extract, namely, to use it as a plant growth regulator to promote plant growth.

[0006] The present invention further provides a plant growth regulator, which contains monk fruit extract.

[0007] The extraction method of monk fruit extract is a commonly used method, such as the method disclosed in CN106349315B: (1) fresh monk fruit or dried monk fruit is selected for swelling, the juice is spirally squeezed out, and pasteurized, and then pectinase, amylase, and cellulase are added. 3 ml of pectinase, 6 ml of amylase, and 8 ml of cellulase are added to each liter of juice, and the mixture is kept at 30-40°C for 2 hours for enzymolysis; (2) the juice obtained in step (1) is concentrated in a vacuum concentrator to a specific density of 1 .2; (3) adding 90%-95% ethanol to make the alcohol content of 65%, letting it stand to precipitate, extracting the supernatant, and concentrating it until there is no alcohol taste and the specific density is 1.15; (4) adding pure water to the liquid obtained in step (3), and adding an appropriate amount of activated carbon, boiling for 20 minutes, cooling and filtering to obtain a water-precipitated supernatant, and concentrating it to a specific density of 1.2 (5) adding acetone to the liquid obtained in step (4) to make the acetone concentration (V / V) reach 50-75%, and precipitate; (6) removing acetone.

[0008] Other traditional methods of preparing monk fruit extract are also acceptable.

[0009] The main active ingredient of the monk fruit extract of the plant growth regulator provided by the invention is mogroside V.

[0010] Furthermore, the content of mogroside V in the plant growth regulator may be 1-60%, and in one embodiment the specific content is 5%, and in another embodiment the specific content may be 8%.

[0011] Furthermore, the plant growth regulator may also contain Momordica grosvenori polysaccharide P1 and Momordica grosvenori polysaccharide P2.

[0012] Furthermore, the plant growth regulator also includes manganese (2-5wt%), zinc (1-3wt%), boron (0.2-0.5wt%), iron (2-4wt%), copper (0.5-0.8wt%), and molybdenum (0.005-0.001wt%).

[0013] Furthermore, the plant growth regulator may also contain other ingredients, such as chelating agents, synergists, cosolvents, and the like.

[0014] The method for using the plant growth regulator is as follows: spraying the plant growth regulator on plants every 7-15 days, and the number of spraying times is more than 3 times.

[0015] The growth regulator is also used to increase plant yield, and / or improve plant survival rate, and / or improve the quality of fresh fruit and / or extend the storage time of fresh fruit.

[0016] The invention uses monk fruit extract as a carrier, adds a variety of trace elements in a scientific ratio, and adopts advanced chelation technology to prepare a new type of composite monk fruit extract agricultural preparation product for enhancing crop stress resistance, increasing crop yield, and improving crop quality.

[0017] The present invention also provides a method for using a plant growth regulator, wherein the plant growth regulator is sprayed on plants every 7-15 days for more than 3 times. If the plant is a transplanted plant, the spraying begins 10 days after transplanting.

[0018] The beneficial effects of the present invention are that the growth of plants is promoted, the yield is increased, and it is friendly to human body, environment and plants without any harm. DETAILED DESCRIPTION

[0019] Example 1 Application of Growth Regulators in Planting Chinese Cabbage

[0020] Test variety: Chinese cabbage.

[0021] Test site soil

[0022] The experiment was conducted in Xingye County Jufeng Breeding and Farming Professional Cooperative in Shatang Village, Shatang Town, Xingye County. The experimental plot was Zhuyu purple sand field with deep tillage layer, convenient irrigation and drainage, pH 5.8, organic matter 22.3g / kg, total nitrogen 1.28g / kg, available phosphorus 13.2mg / kg, and quick-acting potassium 67mg / kg.

[0023] Experimental design

[0024] The experiment was based on conventional fertilization, with three treatments, three replicates, and randomized block arrangement. The area of ​​the plot was 30.0 m 2 , 750 plants per plot, the treatment contents are as follows:

[0025] Treatment group 1: spraying the plant growth regulator of the present invention. The first spraying was performed on the 10th day after transplanting, and then once every 7 days, for a total of three sprayings. Each time, 5g of the plant growth regulator of the present invention was diluted with 50kg of water per mu for foliar spraying. In this embodiment, the content of mogroside V in the plant growth regulator was 5%.

[0026] Treatment group 2: spraying of commercially available plant growth regulator (0.0075% brassinolide), the first spraying was on the 10th day after transplanting, and then once every 7 days, for a total of three sprayings, with a spraying concentration of 0.01 mg / kg.

[0027] Treatment group 3: spray with clean water. The spraying time, number of times and amount are the same as those in treatment 1.

[0028] Conventional fertilization: 60kg of 45% compound fertilizer (15-15-15) and 10kg of urea per mu. Among them: base fertilizer, apply 40kg of compound fertilizer; topdressing, apply 10kg of compound fertilizer and urea each on the 7th day after transplanting; apply 10kg of compound fertilizer on the 21st day.

[0029] Test process

[0030] In this experiment, the land was prepared and divided into plots on July 17, 2023, and transplanting was carried out on July 18, with 750 plants in each plot. The seedlings all had 4 to 5 leaves at the time of transplanting.

[0031] Base fertilizer: On July 17, each plot applied 40 kg of compound fertilizer per mu. Topdressing: On July 24, each plot applied 10 kg of compound fertilizer plus urea per mu; on August 7, each treatment applied 10 kg of compound fertilizer per mu. Sprinkle with clean water after each fertilization.

[0032] Spraying time: According to the test requirements, the plant growth regulator of the present invention was sprayed on treatment 1 on July 27, August 3, and August 10, respectively, and the same amount of water as that of treatment 1 was sprayed on treatment 2; nothing was sprayed on treatment 3.

[0033] Yield measurement and acceptance were conducted on August 28, and acceptance was based on full harvest in the plot. Before harvest, 10 plants were randomly selected from each treatment to examine the biological traits of Chinese cabbage.

[0034] Results and analysis: Effects of different treatments on the biological traits of Chinese cabbage

[0035] Table 1 Effects of different treatments on the biological traits of Chinese cabbage

[0036] deal with Plant height (cm) Single plant weight (g) Treatment Group 1 26.2 107.9 Treatment Group 2 23.9 98.0 Treatment Group 3 23.2 96.5 Treatment 1 vs Treatment 2 2.3 9.9 Treatment 1 vs. Treatment 3 3.0 11.4

[0037] From Table 1, the plant height and single plant weight of treatment 1 increased by 2.3 cm and 9.9 g respectively compared with treatment 2, and increased by 3.0 cm and 11.4 g respectively compared with treatment group 3.

[0038] Effects of different treatments on the yield of Chinese cabbage

[0039] Table 2. Unit of record of plot yield: kg

[0040] Duplicate processing I Ⅱ Ⅲ average Yield per mu 1 78.3 84.2 80.3 80.9 1798.6 2 73.8 71.6 75.1 73.5 1633.4 3 72.1 71.8 73.3 72.4 1609.0

[0041] ANOVA

[0042] Table 3: ANOVA table

[0043]

[0044] The variance analysis and F test showed that the differences among treatments were significant (F=11.83>F0.05).

[0045] Multiple comparisons

[0046] Table 4: Comparison of new complex range

[0047]

[0048] Compared with treatment 2, the yield per mu increased by 165.2 kg, an increase of 10.1%, and the difference was significant; compared with treatment 1 and treatment 3, the yield per mu increased by 189.6 kg, an increase of 11.8%, and the difference was significant.

[0049] in conclusion

[0050] The test results show that the yield per mu of Chinese cabbage sprayed with the plant growth regulator of the present invention is 1798.6 kg, which is 165.2 kg higher per mu than that of spraying with a commercially available plant growth regulator (0.0075% brassinolide), with an increase of 10.1%, and 189.6 kg higher per mu than that of spraying with water, with an increase of 11.8%. The biostatistical test reached a significant level.

[0051] Example 2 Application of Growth Regulators in Strawberry Planting

[0052] Overview of the test site

[0053] The experiment was arranged at the greenhouse strawberry base of Wuhan Mulan Jinqiu Agricultural Science and Technology Development Co., Ltd. in Huangpi District, Wuhan City.

[0054] Test materials

[0055] The strawberry variety tested was "Hongyan".

[0056] Experimental design

[0057] The experiment was divided into two parts: a small-area experiment and a large-area comparative experiment. The small-area experiment was arranged at 335m 2 The experiment was conducted in a greenhouse with two treatments. Treatment 1 was a plant growth regulator extracted from monk fruit: starting from 7 days after planting, the plant growth regulator was sprayed every 10 to 15 days. The concentration was 50 kg of water per 5 g of monk fruit extract, for a total of 6 times. The content of mogroside V in the monk fruit extract was 5%. Treatment 2 was the control, with the conventional fertilization plan of Wuhan Mulan Jinqiu Agricultural Science and Technology Development Co., Ltd. in Huangpi District, Wuhan City, repeated 3 times, and the area of ​​each plot was 8.5 m 2(0.85m×10m), planted about 80 strawberries. The regional comparative test was implemented at 1250m 2 The greenhouse is consistent with the plot experiment. The plot areas of treatment 1 and treatment 2 are 637.5m 2 and 556.2m 2 , no repetition.

[0058] Field management

[0059] The strawberry was fertilized on August 31, and transplanting was 100% completed on September 12. The span of the greenhouse is 8m and the length is 50m. There are 9 beds in the greenhouse, 2 rows in each bed, and about 6,000 plants per mu. Field operations such as pest control were carried out in accordance with the conventional management and cultivation of Wuhan Mulan Jinqiu Agricultural Science and Technology Development Co., Ltd. in Huangpi District, Wuhan. Biological traits such as growth, leaf color, fruit color, taste and flavor were recorded, and yield and commercial properties were measured. Sampling and calculation were carried out in accordance with conventional survey requirements.

[0060] Results and Analysis

[0061] The effect of spraying Momordica extract plant growth regulator on strawberry growth and plant survival rate can be seen from Table 1. The plant mortality rates of treatment 2 and treatment 1 were 26.89% and 19.21%, respectively. The mortality rate of strawberry decreased by 7.68% after spraying Momordica extract plant growth regulator. It can be seen that spraying Momordica extract plant growth regulator can significantly improve the survival rate of strawberry plants and reduce their mortality rate. At the same time, it was found through comparative observation that after spraying Momordica extract plant growth regulator, the branches of strawberry plants became thicker, the leaves were darker green than the control, the leaves were plump, the growth was more luxuriant, and the fruit rate of strawberry in the same area was higher.

[0062] Table 1 Survival rate of strawberry plants in each treatment

[0063]

[0064] Table 2 Weight and sugar content of strawberries in each treatment

[0065]

[0066] Table 3. Strawberry yield and income of each treatment

[0067]

[0068] Effects of spraying Momordica grosvenori extract plant growth regulator on weight and sugar content of strawberry

[0069] About 20 kg of strawberries were randomly picked from two treatments and weighed. It was found that the weight of large, small and defective fruits in treatment 2 accounted for 45.13%, 43.14% and 11.73% of the total weight, respectively. After spraying the plant growth regulator of monk fruit extract, the large, small and defective fruits of strawberries in treatment 1 accounted for 54.27%, 35.67% and 10.06% of the total weight, respectively. Compared with treatment 2, the proportion of large fruits increased by 9.14%, and the proportion of small and defective fruits decreased by 7.47% and 1.66%, respectively. It can be seen that spraying the plant growth regulator of monk fruit extract can increase the weight of strawberry fruits, reduce the number of small and defective fruits, and make the strawberries look large and shiny in appearance, and the quality is more uniform. As shown in Table 2, the sugar content values ​​of strawberries in treatments 1 and 2 are 95.4 and 92.5, respectively. It can be seen that after spraying the plant growth regulator of monk fruit extract, the sugar content value of strawberries increased by 2.9, the sugar content of strawberries was significantly improved, and the taste was better.

[0070] Effect of spraying Momordica grosvenori extract plant growth regulator on the shelf life of strawberries

[0071] On January 25, 2022, two strawberries were picked and stored at room temperature. On February 5, 10 days after the strawberries were picked, some strawberries in treatment 2 began to mold and deteriorate, while the strawberries in treatment 1 did not change much compared with 10 days ago, and the freshness was good, without any tendency to mold and deteriorate; after 20 days of storage, about 50% of the strawberries in treatment 2 were completely moldy, and the remaining 50% also began to wilt, and the glossiness decreased, while only a few strawberries in treatment 1 were moldy, and the rest of the strawberries were fresh and intact, with no obvious changes in appearance; on the 28th day, all the strawberries in treatment 2 were moldy and deteriorated, and except for a very small number of strawberries in treatment 1 that were completely moldy, the rest of the strawberries only began to wilt and shrivel, and some of them did not show significant changes in size and shape. It can be seen that after spraying the monk fruit extract plant growth regulator, the storage time of strawberries is extended, and the spraying of monk fruit extract plant growth regulator has a significant effect on the preservation and quality of strawberries.

[0072] Effects of spraying Momordica grosvenori extract plant growth regulator on strawberry yield and profit

[0073] In the regional comparative test, the strawberry planting areas of treatments 1 and 2 were 637.5 and 556.2 m 2, picking strawberries began on December 10, 2021, and ended on April 7, 2022. As can be seen from Table 3, during the same picking period, the total strawberry yields of treatments 1 and 2 were 639.4 and 459.5 kg, respectively, which were converted into per mu yields of 668.7 and 550.8 kg, respectively. After spraying monk fruit extract plant growth regulators, the increase in strawberry yield reached 21.41%. At the same time, due to the increase in unit yield and quality improvement of strawberries, the per mu output value increased from 13,961.3 yuan to 18,054.9 yuan, and the per mu income increased by 4,093.6 yuan, an increase of 29.32%. It can be seen that spraying monk fruit extract plant growth regulators can significantly increase the yield of strawberries and increase the economic benefits of farmers. It can be seen that spraying monk fruit extract plant growth regulators has a significant effect on increasing the yield and efficiency of strawberries.

[0074] in conclusion

[0075] After spraying monk fruit extract plant growth regulator, the mortality rate of strawberry plants decreased, their stress resistance increased, and their growth became more vigorous; the appearance of the strawberries became more uniform, the sugar content was higher, the shelf life was extended, and the taste was better; the strawberry yield increased, and the farmers' economic income also increased significantly.

[0076] Example 3 Application of Growth Regulators on Sugar Oranges

[0077] Test crops

[0078] For 7-year-old mandarin oranges, the row spacing is 3 meters x 2.5 meters, and 90 plants are planted per acre.

[0079] Test site and soil conditions

[0080] The experiment was carried out in Hequn Village, Tanxia Town, Lingchuan County, Guilin City, Guangxi. The soil is a paddy soil developed from sandstone shale parent material. The texture is medium loam, the fertility is medium and consistent, and it is easy to drain and irrigate. There are 18.3g / kg organic matter, 1.1g / kg total nitrogen, 17.8mg / kg available phosphorus (P205), 73.1mg / kg available potassium (K20), and PH5.3.

[0081] Experimental design

[0082] The experiment had three treatments and three replications: the plot area was 37.5 m2 (5 plants).

[0083] Treatment 1: local conventional fertilization + monk fruit extract plant growth regulator.

[0084] Treatment 2: Local conventional fertilization + clean water control:

[0085] Treatment 3: Local routine fertilization.

[0086] Treatment 1: Spraying Luo Han Guo extract plant growth regulator. Spraying method: Spray once during the flowering period, fruit setting period, fruit expansion period, and before fruit picking. The content of mogroside V in the plant growth regulator is 4.8%, the concentration of each spraying is 1:800, and the spraying amount per mu is 60 kg.

[0087] Treatment 2: Spray with clean water. The spraying time, method and amount of water per unit area are the same as those in the demonstration area.

[0088] Field management

[0089] Fertilization

[0090] In Lingchuan County, sugar oranges are generally fertilized with restorative fertilizers after the fruits are harvested in the previous year, and in the next year, flower and fruit preservation fertilizers and fruit strengthening and shoot promotion fertilizers are applied according to the season.

[0091] Pest and disease control

[0092] Throughout the year, we focus on controlling spiders, leaf beetles, aphids, rust-proof wall phoenixes, giant flies, leaf miners and other pests and diseases such as rust and sooty mold. Starting from April, we sprayed monk fruit extract plant growth regulator or an equal amount of clean water at different times according to the requirements of the demonstration treatment, for a total of 4 times, in combination with each pest and disease control.

[0093] weeding

[0094] 41% glyphosate (Roundup) was used to control weeds three times throughout the year, in early April, mid-August and late September.

[0095] Harvest

[0096] The yield measurement was carried out on January 18, 2023. The trial harvest was carried out in divided plots and whole areas as required, and the yield of sugar mandarin oranges was calculated individually.

[0097] Results and Analysis

[0098] Cell Trial

[0099] Effects of different treatments on biological characteristics of sugar orange

[0100] Table 1 Effects of different treatments on biological traits of sugar orange

[0101]

[0102]

[0103] As shown in Table 1, compared with the control area, the average number of fruits per plant increased by 100-116, and the average single fruit weight increased by 3.5-4.1g after spraying monk fruit extract plant growth regulator.

[0104] Yield Results

[0105] Table 2 Statistics of each treatment acceptance yield

[0106]

[0107] ANOVA

[0108] According to the analysis of the yield of each treatment plot, the results showed that the F value (55.93) among treatments was greater than F0.01 (18.00), indicating that the difference among the experimental treatments reached an extremely significant level (see Table 4).

[0109] Table 3 Analysis of variance of yield of each treatment

[0110] Causes Degrees of Freedom sum of squares Mean Square F-number <![CDATA[F 0.05 ]]> <![CDATA[F 0.01 ]]> Processing room 2 1107253.48 553626.74 55.93 6.94 18.00 Repeating 2 8037.59 4018.79 0.41 error 4 39591.60 9897.90 Total variation 8 1154882.67

[0111] Multiple comparisons

[0112] After multiple comparisons using the new multiple range method, it can be seen that there are extremely significant differences between treatment 1 and treatments 2 and 3, but there is no significant difference between treatment 2 and treatment 3 (see Table 6).

[0113] Table 5 New complex range comparison table

[0114] P 2 3 SSR0.05 3.93 4.01 SSR0.01 6.51 6.68 LSR0.05 225.74 230.33 LSR0.01 373.93 390.59

[0115] Table 6 Results of significant difference test for different treatments

[0116] deal with Average output Comparison with treatment 3 Comparison with treatment 2 1 5861.6 800.6** 670.2** 2 5191.4 130.4 - 3 5061.0 - -

[0117] Economic Benefit Analysis

[0118] The comparison results of yield and output value of different treatments are shown in Table 7.

[0119] Table 7 Input-output analysis of different treatments

[0120]

[0121] Note: The price of sugar orange is 6.00 yuan / kg. The plant growth regulator extracted from monk fruit in this experiment is 60 yuan / mu / time, and the labor cost is 30 yuan / mu / time. The labor cost of conventional fertilization and field management is the same for each treatment, and the two are offset after comparison and not included.

Claims

1. The application of monk fruit extract as a growth regulator is characterized in that: The monk fruit extract is used as a plant growth regulator to promote the growth of plants.

2. The use of the monk fruit extract as claimed in claim 1 as a growth regulator, characterized in that: The plant growth regulator includes monk fruit extract.

3. The use of the Momordica grosvenori extract as claimed in claim 1 as a growth regulator, characterized in that: The content of mogroside V in the plant growth regulator is 1-60%.

4. The use of the monk fruit extract as claimed in claim 1 as a growth regulator, characterized in that: The plant growth regulator also includes Momordica grosvenori polysaccharide P1 and / or Momordica grosvenori polysaccharide P2.

5. The use of the Momordica grosvenori extract as a growth regulator according to claim 1, characterized in that: The plant growth regulator also includes manganese, zinc, boron, iron, copper and / or molybdenum.

6. The use of the monk fruit extract as a growth regulator according to claim 1, characterized in that: The plant growth regulator also includes a chelating agent, a synergist and / or a cosolvent.

7. The use of the monk fruit extract as a growth regulator according to any one of claims 2 to 6, characterized in that: The method for using the plant growth regulator is as follows: spraying the plant growth regulator on plants every 7-15 days, and the number of spraying times is more than 3 times.

8. The use of the monk fruit extract as a growth regulator according to any one of claims 2 to 6, characterized in that: The growth regulator is also used to increase plant yield, and / or improve plant survival rate, and / or improve the quality of fresh fruit and / or extend the storage time of fresh fruit.

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