A composite microbial feed additive and its production equipment

Abstract

The present invention relates to the technical field of feed additives, and in particular to a composite microbial feed additive and a production device thereof, wherein the composite microbial feed additive includes Bacillus LB1#, yeast extract, lactic acid bacteria and Bacillus megaterium LB2#; the composite microbial feed additive production device includes a mixing tank, a mixing assembly is provided inside the mixing tank, the mixing assembly includes a driving rod, the driving rod is externally rotatably connected to a positioning rod, the positioning rod is externally rotatably connected to a rotatable rotating telescopic rod, and a plurality of stirring paddles are circumferentially hinged on the rotating telescopic rod. By this, the present invention can promote the growth and development of organisms, ensure the stability of microbial feed additives, and when producing biological feed additives, the inclination angle and position of the stirring paddle can be adjusted at any time, and the microbial liquid and the main material can be evenly mixed by using the movable stirring paddle to improve the mixing effect.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and in particular to a composite microbial feed additive and production equipment thereof. Background Art

[0002] With the rapid development of livestock and poultry farming in my country, large-scale farming is the general trend of livestock and poultry farming, among which scientific and technological farming also has a wider application space. After the promulgation of the "Antibiotic Ban", the research and development of green alternative antibiotic feed additives are encouraged to ensure the healthy and green development of my country's breeding industry. However, the existing alternative antibiotic feed additives have the disadvantages of poor performance and high cost.

[0003] Microbial feed additives are a type of preparation that is non-toxic, has no side effects, is non-resistant, and has no environmental pollution. They not only maintain the balance of intestinal microecology, promote animal growth, and improve feed conversion rate and animal breeding efficiency, but also some functional bacteria can produce antimicrobial peptides, which have the effects of inhibiting harmful bacteria, enhancing the body's immune function, and reducing animal mortality. They are playing an increasingly important role in replacing antibiotics and reducing the use of chemical synthetic drugs in livestock and poultry farming, and are increasingly becoming the development trend of feed additives. However, the performance of existing microbial feed additives is very unstable. Microbial preparations will be inactivated in large quantities when they pass through the strong acid environment of the stomach and then reach the high bile salt environment of the intestine. The number of surviving microorganisms is very small, and it is difficult to exert their effects, which affects the quality of the microbial preparations. In addition, during the processing of microbial feed additives, the mixing effect of the mixed microbial culture and the main ingredient is poor, and the production efficiency is low.

[0004] How to promote the growth and development of organisms, ensure the stability of microbial feed additives, and adjust the inclination angle and position of the stirring paddle at any time when producing biological feed additives, and use the movable stirring paddle to mix the microbial liquid with the main material evenly to improve the mixing effect has become a technical problem that needs to be broken through.

[0005] In summary, the prior art obviously has inconveniences and defects in practical use, so it is necessary to improve it. Summary of the invention

[0006] In view of the above-mentioned defects, the purpose of the present invention is to provide a composite microbial feed additive and its production equipment, which can promote the growth and development of organisms, ensure the stability of the microbial feed additive, and when producing the biological feed additive, the inclination angle and position of the stirring paddle can be adjusted at any time, and the microbial liquid and the main material can be mixed evenly by using a movable stirring paddle to improve the mixing effect.

[0007] In order to achieve the above object, the present invention provides a composite microbial feed additive, comprising Bacillus LB1#, yeast extract, lactic acid bacteria and Bacillus LB2#.

[0008] The Bacillus LB1#, Latin name: Bacillus sp., was deposited in the General Microbiology Center of China Culture Collection Administration on November 15, 2021. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23914.

[0009] The Bacillus LB2#, Latin name: Bacillus sp., was deposited in the General Microbiology Center of China Culture Collection Administration on November 15, 2021. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23915.

[0010] According to the composite microbial feed additive of the present invention, the mass ratio of the Bacillus LB1#, yeast extract, lactic acid bacteria and Bacillus LB2# is 1:1:1:1.

[0011] The present invention also provides a composite microbial feed additive production device, including a mixing tank, a mixing assembly is arranged inside the mixing tank, the mixing assembly includes a rotatable driving rod rotatably connected to the inside of the mixing tank, the outside of the driving rod is rotatably connected to a positioning rod, the outside of the positioning rod is rotatably connected to a rotatable rotating telescopic rod, and a plurality of stirring paddles are hinged on the rotating telescopic rod along the circumferential direction.

[0012] The rotating telescopic rod is rotatably connected to the moving rod, and the moving rod is sleeved on the outside of the positioning rod. The positioning rod is provided with a plurality of hollow grooves along the circumferential direction. The moving rod is threadedly connected to the driving rod. A first positioning plate and a second positioning plate are sleeved on the outside of the rotating telescopic rod close to the moving rod. The first positioning plate and the second positioning plate are respectively provided with a movable first adjusting member and a second adjusting member at one end away from the stirring paddle, and the first adjusting member and the second adjusting member are both provided with an avoidance groove at one end close to the stirring paddle.

[0013] During the material mixing process, in order to prevent uneven mixing caused by material sedimentation, the driving rod is rotated to move the moving rod on the positioning rod, and the position of the moving rod is adjusted. At the same time, by adjusting the positions of the first adjusting member and the second adjusting member, the inclination angles of several stirring paddles are adjusted to improve the mixing effect.

[0014] According to the composite microbial feed additive production equipment of the present invention, the cross-sectional area of ​​the avoidance groove of the first adjusting member is greater than the cross-sectional area of ​​the avoidance groove of the second adjusting member.

[0015] According to the composite microbial feed additive production equipment of the present invention, the rotating telescopic rod comprises an outer rod and an inner rod which are sleeved with each other.

[0016] According to the composite microbial feed additive production equipment of the present invention, a plurality of first telescopic parts are provided between the first positioning plate and the inner bottom wall of the avoidance groove of the first adjusting part, a plurality of second telescopic parts are provided between the second positioning plate and the inner bottom wall of the avoidance groove of the second adjusting part, and the first adjusting part is sleeved on the outside of the inner rod, and the second adjusting part is sleeved on the outside of the movable rod.

[0017] According to the composite microbial feed additive production equipment of the present invention, a plurality of through holes are provided on the inner bottom wall of the avoidance groove of the second adjusting member.

[0018] According to the composite microbial feed additive production equipment of the present invention, a fixed box is provided inside the mixing tank, one end of the driving rod, the positioning rod and the rotating telescopic rod are all inserted into the fixed box, and a driven bevel gear is sleeved on the outer rod inside the fixed box, and the driven bevel gear meshes with a rotatable driving bevel gear.

[0019] The purpose of the present invention is to provide a composite microbial feed additive, including Bacillus LB1#, yeast extract, lactic acid bacteria, and Bacillus LB2#, which can promote the growth and development of organisms and ensure the stability of microbial feed additives; the present invention also provides a production device for composite microbial feed additives, including a mixing component, by adjusting the positions of the first adjusting member, the second adjusting member, and the movable rod, the inclination angle and position of the stirring paddle can be adjusted at any time, and the microbial liquid and the main material can be mixed evenly by using the movable stirring paddle to improve the mixing effect. In summary, the beneficial effects of the present invention are: it can promote the growth and development of organisms, ensure the stability of microbial feed additives, and when producing biological feed additives, the inclination angle and position of the stirring paddle can be adjusted at any time, and the microbial liquid and the main material can be mixed evenly by using the movable stirring paddle to improve the mixing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural diagram of the production equipment of the present invention; Figure 2 It is a longitudinal section view of the interior of the mixing tank; Figure 3 for Figure 2 Enlarged view of part A in the middle; Figure 4 for Figure 2 Enlarged view of part B; Figure 5 This is the structural diagram of the stirring paddle; Figure 6 for Figure 5Enlarged view of part C in the middle; in the figure: 1-mixing tank, 11-feed port, 12-discharge port, 2-mixing assembly, 21-fixed box, 211-active bevel gear, 212-second driving member, 22-driving rod, 23-positioning rod, 231-hollow groove, 24-rotating telescopic rod, 241-outer rod, 242-inner rod, 243-stirring paddle, 244-driven bevel gear, 25-moving rod, 26-first positioning plate, 261-first telescopic member, 262-first adjusting member, 27-second positioning plate, 271-second telescopic member, 272-second adjusting member, 273-through hole, 3-first driving member. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0022] In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", "top / bottom" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installed", "provided with", "mounted / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection. It can be a mechanical connection or an electrical connection. It can be a direct connection or an indirect connection through an intermediate medium. It can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] The invention provides a composite microbial feed additive, comprising bacillus LB1#, yeast extract, lactic acid bacteria and bacillus LB2#, wherein the mass ratio of bacillus LB1#, yeast extract, lactic acid bacteria and bacillus LB2# is 1:1:1:1.

[0025] The above-mentioned Bacillus LB1#, Latin name: Bacillus sp., was deposited in the General Microbiology Center of China Culture Collection Administration on November 15, 2021. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23914.

[0026] The above-mentioned Bacillus LB2#, Latin name: Bacillus sp., was deposited in the General Microbiology Center of China Culture Collection Administration on November 15, 2021. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23915.

[0027] The screening methods of the above-mentioned Bacillus LB1# and Bacillus LB2# both comprise the following steps:

[0028] Step 1: Sample collection

[0029] Take saline-alkali soil, mix the sterile PBS buffer with the saline-alkali soil (add 5 mL PBS buffer for every 1 g of saline-alkali soil), heat in a water bath at 85°C for 20 min, take 1 mL of the supernatant and perform a 10-fold series of gradient dilutions to obtain a series of bacterial dilutions with different concentrations of 10-1 to 10-4. Since Bacillus has a certain degree of high temperature resistance, the impurities fungi and bacteria in the PBS buffer after heating in a water bath will be eliminated to the greatest extent.

[0030] Step 2 Isolation of strains

[0031] The dilutions of the bacterial source at each dilution were aspirated and inoculated on the surface of the TSA plate. Three dilutions were inoculated on each plate and cultured at 37°C for 24 h to obtain single colonies.

[0032] Step 3: Purification of isolated bacteria

[0033] Pick a single colony from the above plate, streak inoculate on a TSA plate, and culture at 37°C for 24 h. Repeat the streak inoculation and culture three times to obtain pure cultured isolated bacteria.

[0034] In order to verify the above isolated bacteria, morphological identification and gene sequence identification were performed on the above isolated bacteria:

[0035] Morphological identification

[0036] Take the isolated bacterial liquid, dilute it with an appropriate amount of physiological saline, and then smear it for Gram staining and spore staining. Observe the bacterial staining and morphological characteristics under a microscope. The isolated bacteria appear milky white and opaque on the TSA plate, with irregular edges and wrinkles on the surface, and are easy to pick up.

[0037] Microscopic examination after Gram staining:

[0038] LB1# morphology: The colonies were light yellow, round, and moist on the surface. Microscopic examination showed that they were syngenesial bacteria. The strain was identified as Bacillus.

[0039] LB2# morphology: The colonies were light yellow, round, and moist on the surface. Microscopic examination showed conjoined filamentous fungi. The strain was identified as Bacillus and was presumed to be Bacillus megaterium.

[0040] Gene sequence identification

[0041] The isolated bacterial strains were sent to Shanghai Biotech Co., Ltd. for sequencing. The obtained 16S r DNA sequence information was compared with the 16S database of NCBI, and the parameter identify>95 was set. Then the top 30 16S r DNA sequences with the highest identify were selected (all were taken if insufficient), and the sequences were multiple aligned using muscle software, and the phylogenetic tree was constructed using FastTree software. The results showed that the isolated bacterial strains were closest to Bacillus. Therefore, the isolated bacterial strains were classified as Bacillus and named Bacillus LB1# and Bacillus LB2# respectively.

[0042] The preparation method of the composite microbial feed additive of the present invention comprises the following steps:

[0043] Bacillus LB1#, yeast extract, lactic acid bacteria, Bacillus LB2# and water are stirred and mixed for 10 to 20 minutes (while controlling the temperature to be 20 to 30° C.) to obtain fermentation strains, and the fermentation strains are mixed with fermentation medium and then solid-state fermented to obtain a composite microbial feed additive.

[0044] Specifically, the fermentation medium includes corn flour, soybean meal powder and yeast protein powder, and the weight ratio of corn flour, soybean meal powder and yeast protein powder is 10:10:1.

[0045] In order to verify the effect of the above-mentioned composite microbial feed additive, the present invention provides the following examples:

[0046] The above-mentioned composite microbial feed additives of different weights are mixed with ordinary feed (conventional feed containing fish meal, soybean meal, corn meal, fish oil, vitamins and other components). After all the feed raw materials are mixed evenly, water is sprayed to prepare dough, which is extruded and puffed into shape using a feed pellet machine, shaved in the shade at room temperature until semi-dried, cut into pellets, and stored in a cool and dry place.

[0047] 1200 Litopenaeus vannamei fry (body length of about 3.0±0.2cm) with a small difference in body length were selected and divided into 4 groups: control group (the amount of compound microbial feed additives added was 0), experimental group 1 (the amount of compound microbial feed additives added was 0.5% of the total feed amount), experimental group 2 (the amount of compound microbial feed additives added was 1% of the total feed amount) and experimental group 3 (the amount of compound microbial feed additives added was 3% of the total feed amount). Each group had 3 replicates, and each replicate group had 100 shrimps. The experimental period was 4 weeks. During the breeding period, the shrimps were fed 4 times a day, and the residual bait and feces were removed regularly. The food intake and health status were recorded. Aeration was uninterrupted throughout the breeding process.

[0048] During the breeding experiment, 10 shrimps were randomly selected from each tank every week to measure their body length and weight. After the experiment, the shrimps in each tank were counted to calculate the survival rate, weight gain rate and fullness, where survival rate (%) = number of surviving shrimps / total number of shrimps × 100; weight gain rate (%) = (W1-W0) / W0×100, W0 is the initial weight of shrimps, W1 is the final weight of shrimps after the experiment; fullness (g / cm 3 )=W1 / L 3 ×100, W1 is the final weight of the shrimp after the test, L is the final body length of the shrimp after the test; after removing the error of the repeated groups of individual experimental groups, the measured data are shown in the following table:

[0049] Table 1 Effects of compound microbial feed additives on growth performance of Litopenaeus vannamei

[0050] variable Initial body weight (g) Final weight (g) Initial body length (cm) Final body length (cm) Survival rate (%) Weight gain rate (%) <![CDATA[Saturation (g / cm 3 ).]]> Control group 0.31 3.53 3.38 8.21 78.11 1038.70 0.64 Experimental Group 1 0.31 4.55 3.38 8.78 79.34 1367.74 0.67 Experimental Group 2 0.31 4.87 3.38 8.94 82.41 1470.96 0.68 Experimental Group 3 0.31 4.71 3.38 8.91 80.36 1419.35 0.67

[0051] It can be seen from the data in the above table that experimental group 2 (i.e., the addition of the composite microbial feed additive accounted for 1% of the total feed amount) had the best effect. In the data in the above table, due to possible errors in the measurement of shrimp, the initial body weight and initial body length of each group were controlled within ±0.02, and the measurements of the above initial body weight, final body weight, initial body length and final body length were all accurate to 0.01 to reduce the errors caused by the experiment.

[0052] See also Figure 1 , Figure 2 and Figure 4During the experiment, there were some differences in the growth conditions of the shrimps in the replicate groups of the individual experimental groups. After repeated experiments, it was found that the feed used in the replicate groups with errors was not completely mixed. In order to improve the mixing effect of the composite microbial feed additive and the ordinary feed, the present invention also provides a production device for the composite microbial feed additive, including a mixing tank 1 (except for the structure of the stirring material inside the mixing tank 1, the rest of the structure is the same as the prior art, including a feed inlet 11, a discharge outlet 12 and a heating device, etc., which can regulate the temperature and pressure in the mixing tank 1 during the mixing process. It is a conventional device for those skilled in the art, and its specific structure The structure and working principle are not repeated here), a mixing assembly 2 is arranged inside the mixing tank 1, and the mixing assembly 2 includes a fixed box 21 connected to the inside of the mixing tank 1, and a driving rod 22 is penetrated in the fixed box 21, and one end of the driving rod 22 passes through the mixing tank 1 to connect with the first driving member 3, and the first driving member 3 is connected to the mixing tank 1, and the first driving member 3 can drive the driving rod 22 to rotate, and the other end of the driving rod 22 passes through the fixed box 21 and is rotatably connected to the inside of the mixing tank 1, and the outer part of the driving rod 22 is rotatably connected to the positioning rod 23, and the positioning rod 23 is connected to the fixed box 21, and the outer part of the positioning rod 23 is rotatably connected to the rotating telescopic rod 24, and the rotating telescopic rod 24 includes outer sleeves. The outer rod 241 is provided with a driven bevel gear 244, and the driven bevel gear 244 can drive the outer rod 241 to rotate. The driven bevel gear 244 meshes with the active bevel gear 211, and the active bevel gear 211 can drive the driven bevel gear 244 to rotate. The gear 244 rotates, the active bevel gear 211 is connected to the second driving member 212, the second driving member 212 can drive the active bevel gear 211 to rotate, the second driving member 212 is connected to the inner wall of the fixed box 21, and a plurality of stirring paddles 243 are hinged on the inner rod 242 outside the fixed box 21 along the circumferential direction, and the inner rod 242 can drive the plurality of stirring paddles 243 to rotate; the second driving member 212 is turned on, the second driving member 212 drives the active bevel gear 211 to rotate, the active bevel gear 211 drives the driven bevel gear 244 to rotate, the driven bevel gear 244 drives the rotating telescopic rod 24 to rotate, thereby driving the plurality of stirring paddles 243 to rotate, and the material in the mixing tank 1 is stirred.

[0053] See also Figure 1 to Figure 6, the end of the telescopic rod 24 away from the fixed box 21 (that is, the end of the inner rod 242 away from the fixed box 21) is rotated to connect the moving rod 25, the moving rod 25 is sleeved on the outside of the positioning rod 23, and the positioning rod 23 is provided with a plurality of hollow grooves 231 along the circumferential direction. The inner circumference of the moving rod 25 is provided with a plurality of inserts that match the hollow grooves 231, and the inserts are all inserted into the hollow grooves 231 and then threadedly connected to the driving rod 22; open the first driving member 3, rotate the driving rod 22, and under the restriction of the hollow grooves 231 of the positioning rod 23, the moving rod 25 The inner rod 242 can be rotated with the outer rod 241, so when the moving rod 25 drives the inner rod 242 to move, the inner rod 242 can still drive the stirring paddles 243 to rotate; the inner rod 242 is provided with a first positioning plate 26 and a second positioning plate 27 on the outer side of the inner rod 242 close to the moving rod 25, and the first positioning plate 26 and the second positioning plate 27 are respectively provided with a first adjustment plate 26 and a second adjustment plate 27 on the ends away from the stirring paddles 243. The first adjusting member 262 and the second adjusting member 272, the first adjusting member 262 is sleeved on the outside of the inner rod 242 (the first adjusting member 262 can be relatively displaced with the inner rod 242), the second adjusting member 272 is sleeved on the outside of the moving rod 25 (the second adjusting member 272 can be relatively displaced with the moving rod 25), the first adjusting member 262 and the second adjusting member 272 are both provided with an avoidance groove at one end close to the stirring paddle 243, and a plurality of first telescopic members are provided between the first positioning plate 26 and the inner bottom wall of the avoidance groove of the first adjusting member 262 261, a plurality of second telescopic members 271 are provided between the inner bottom wall of the avoidance groove of the second positioning plate 27 and the second adjusting member 272, the first telescopic member 261 can drive the first adjusting member 262 to move, and the second telescopic member 271 can drive the second adjusting member 272 to move; by controlling the first telescopic member 261 and the second telescopic member 271, the positions of the first adjusting member 262 and the second adjusting member 272 can be adjusted, so that the inclination angles of the plurality of stirring paddles 243 can be fixed by using the first adjusting member 262 and the second adjusting member 272.

[0054] See also Figure 1 to Figure 6 Preferably, a plurality of through holes 273 are provided on the inner bottom wall of the avoidance groove of the second adjusting member 272 . Since the avoidance groove of the second adjusting member 272 opens upward, the through holes 273 can prevent material from accumulating in the second adjusting member 272 .

[0055] See also Figure 1 to Figure 6 Furthermore, the cross-sectional area of ​​the avoidance groove of the first adjusting member 262 is greater than the cross-sectional area of ​​the avoidance groove of the second adjusting member 272. In daily use, in order to improve the stirring effect and prevent material deposition, the stirring paddle 243 needs to be tilted downward (compared to the horizontal plane). By setting different cross-sectional areas, the movable distance of the first adjusting member 262 can be increased, so that the stirring paddle 243 can be tilted downward.

[0056] See also Figure 1 to Figure 6 Preferably, the first driving member 3 and the second driving member 212 are both rotating motors, and the first telescopic member 261 and the second telescopic member 271 are both electric cylinders to provide power for the device.

[0057] See also Figure 1 to Figure 6 First, according to the capacity and height of the mixing tank 1, the first driving member 3 is turned on to make the moving rod 25 move up and down on the positioning rod 23, and the initial position of the moving rod 25 is set. Then, by controlling the first telescopic member 261 and the second telescopic member 271, the positions of the first adjusting member 262 and the second adjusting member 272 are adjusted, and the inclination angles of the plurality of stirring paddles 243 are fixed by using the first adjusting member 262 and the second adjusting member 272, the microbial liquid and the main material can be introduced into the mixing tank 1, and the second driving member 212 is turned on to drive the plurality of stirring paddles 243 to rotate, so as to stir the material in the mixing tank 1 (at the same time, auxiliary operations such as heating can be performed according to the requirements of the reaction).

[0058] See also Figure 1 to Figure 6 During the mixing process, in order to prevent uneven mixing caused by material sedimentation, the first driving member 3 can be turned on to adjust the position of the moving rod 25. At the same time, by adjusting the positions of the first adjusting member 262 and the second adjusting member 272, the inclination angles of the plurality of stirring paddles 243 can be adjusted to improve the mixing effect.

[0059] The present invention provides a composite microbial feed additive, including Bacillus LB1#, yeast extract, lactic acid bacteria, and Bacillus LB2#, which can promote the growth and development of organisms and ensure the stability of microbial feed additives; the present invention also provides a production device for composite microbial feed additives, including a mixing component, which can adjust the inclination angle and position of the stirring paddle at any time by adjusting the positions of the first adjusting member, the second adjusting member, and the movable stirring paddle to mix the microbial liquid with the main material evenly, thereby improving the mixing effect. In summary, the beneficial effects of the present invention are: it can promote the growth and development of organisms, ensure the stability of microbial feed additives, and when producing biological feed additives, the inclination angle and position of the stirring paddle can be adjusted at any time, and the movable stirring paddle can be used to mix the microbial liquid with the main material evenly, thereby improving the mixing effect.

[0060] Of course, the present invention may have many other embodiments. Without departing from the spirit and essence of the present invention, those skilled in the art may make various corresponding changes and modifications based on the present invention, but these corresponding changes and modifications should all fall within the scope of protection of the claims attached to the present invention.

Claims

1. A composite microbial feed additive, characterized in that: Including Bacillus LB1#, yeast extract, lactic acid bacteria and Bacillus LB2#; The Bacillus LB1#, Latin name: Bacillus sp., was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on November 15, 2021, with the deposit address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23914; The Bacillus LB2#, Latin name: Bacillus sp., was deposited in the General Microbiology Center of China Culture Collection Administration on November 15, 2021. The deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO.23915.

2. The composite microbial feed additive according to claim 1, characterized in that: The mass ratio of the Bacillus LB1#, yeast extract, lactic acid bacteria and Bacillus LB2# is 1:1:1:1.

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