High-efficiency low-irritation peptide-containing antioxidant composition as well as preparation method and cosmetic thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a high-efficiency low-irritation peptide-containing antioxidant composition, a preparation method thereof and cosmetics. The peptide-containing antioxidant composition is prepared from the following components in parts by weight: 1 to 3 parts of carnosine, 0.1 to 0.3 part of hydroxyl pinacolone retinoate and 4 to 12 parts of dimethyl methoxy benzodihydropyranol, and the peptide-containing antioxidant composition has the advantages of excellent antioxidant and anti-aging effects and low irritation.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a high-efficiency, low-irritation peptide-containing antioxidant composition, a preparation method thereof, and cosmetics. Background Art

[0002] Peptides are compounds formed by amino acids linked together by peptide bonds. They are intermediate products of protein hydrolysis, and their molecular structure is between proteins and amino acids. Peptides formed by dehydration and condensation of multiple amino acid molecules are called polypeptides, and those composed of more than 50 amino acids are generally called proteins.

[0003] Peptides can be divided into 1. signal peptides; 2. nerve conduction blocking / inhibitory peptides (botulinum toxin-like); 3. carrier peptides according to their targets.

[0004] For example, palmitoyl pentapeptide-4 is a signal peptide that can mimic the skin's own polypeptide signal and act on fibroblasts to synthesize the extracellular matrix, so as to promote the proliferation of collagen, elastic fiber and hyaluronic acid, and increase the skin's moisture content. It is more suitable for improving static lines caused by collagen loss due to natural aging of the face. This type of peptide has a single target and is relatively limited in application. It is only suitable for specific populations or special targets.

[0005] Dipeptide diaminobutyroylbenzylamide diacetate belongs to the botulinum toxin peptide family and is similar to a signal jammer. It blocks the release of the neurotransmitter acetylcholine in facial muscles, thereby reducing muscle contraction. However, this peptide does not have a significant improvement effect on the skin itself and only has an immediate firming effect.

[0006] Blue copper peptide is a carrier peptide. The copper ion itself is free in the stratum corneum of the skin, but when combined with tripeptide-1, it can act on the dermis of the skin to promote wound healing and hair growth. Although it carries the efficacy of two active ingredients, it is very unstable and has extremely demanding environmental requirements.

[0007] At present, although there are many types of peptides on the market, their efficacy targets are often relatively single, and multiple peptides need to be combined and formulated together to be added to cosmetics to make the cosmetics have more comprehensive efficacy. However, the targets of multiple peptides are intricate and mutually reinforcing, which easily leads to unstable effects and adverse reactions. Therefore, there is currently a lack of a peptide-containing antioxidant composition that has multiple targets, is easier to absorb, and has stable effects.

[0008] Chinese patent application CN111388348A discloses a composition, which uses hydroxypinacolone retinoic acid ester and retinol retinoic acid ester as well as hydroxypropyl tetrahydropyrantriol, niacinamide, tocotrienol, 3-o-ethyl ascorbic acid and lipoic acid for anti-aging. Anti-aging is concerned with people approaching aging, whose body resistance is reduced, and the skin is prevented from further aging by regulating the body's function, while anti-oxidation is suitable for people of all ages. However, the composition only focuses on the anti-aging effect, not the antioxidant effect, and its antioxidant effect needs to be further improved.

[0009] Chinese patent application CN115708797A provides an antioxidant composition, the raw materials for the preparation of the antioxidant composition include ligustrazine, astaxanthin, carnosine, Artemia extract, ectoine, ascorbyl glucoside and vitamin E compounds. The components in the antioxidant composition cooperate with each other, synergistically enhance the effect, and endogenous antioxidant and exogenous antioxidant are combined, but there are many effective ingredients in its formula, and it is uncertain which ingredient is effective and what the effective ratio is, and the antioxidant effect needs to be further improved.

[0010] Therefore, it is very necessary to develop a high-efficiency, low-irritation peptide-containing antioxidant composition and a preparation method and cosmetics thereof that can solve the above-mentioned technical problems. Summary of the invention

[0011] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a peptide-containing antioxidant composition with excellent antioxidant and anti-aging effects and low irritation.

[0012] The present invention is achieved through the following technical solutions: A peptide-containing antioxidant composition comprises the following components, measured by weight: 1-3 parts of carnosine, 0.1-0.3 parts of hydroxypinacolone retinoic acid ester and 4-12 parts of dimethylmethoxychromanol.

[0013] Preferably, the mass ratio of carnosine, hydroxypinacolone retinoic acid ester and dimethylmethoxychromanol is 1:0.1:4-6.

[0014] Preferably, the composition further comprises at least one of a phospholipid emulsifier, a nonionic co-emulsifier, grape seed oil, rice bran oil, meadowfoam seed oil, glycerin and water.

[0015] More preferably, the phospholipid emulsifier comprises hydrogenated lecithin.

[0016] More preferably, the nonionic co-emulsifier includes at least one of Tween-80, Laureth-4, Ceteareth-12, PEG-8 stearate and glyceryl isostearate.

[0017] More preferably, the composition further comprises a phospholipid emulsifier, a nonionic co-emulsifier, grape seed oil, rice bran oil, meadowfoam seed oil, glycerin and water.

[0018] More preferably, the composition comprises, by weight percentage, 1-3% carnosine, 0.1-0.3% hydroxypinacolone retinoic acid ester, 4-12% dimethylmethoxychromanol, 1%-3% phospholipid emulsifier, 0.2%-2% nonionic emulsifier, 1-3% grape seed oil, 1-3% rice bran oil, 1%-10% meadowfoam seed oil, 10%-40% glycerol and the balance water.

[0019] The present invention also relates to a method for preparing the above-mentioned composition, comprising the following steps: (1) mixing carnosine, dimethylmethoxychromanol, phospholipid emulsifier, glycerol and water to obtain an aqueous phase mixture; (2) mixing hydroxypinacolone retinoic acid ester, nonionic emulsifier, grape seed oil, rice bran oil, and meadowfoam seed oil to obtain an oil phase mixture; (3) mixing the aqueous phase mixture and the oil phase mixture, heating and stirring to obtain a premix; (4) The premix is ​​subjected to shear homogenization to obtain colostrum, and then subjected to microfluidization homogenization to obtain colostrum.

[0020] Preferably, the stirring rate in step (1) is 700-800 rpm.

[0021] Preferably, the stirring rate in step (2) is 700-800 rpm, and the stirring temperature is 40-50°C.

[0022] Preferably, the heating temperature in step (3) is 40-50° C. and the stirring rate is 500-600 rpm.

[0023] The heating and stirring in step (3) can also be replaced by ultrasonic homogenization.

[0024] Preferably, the shear homogenization rate in step (4) is 13500-14000 rpm, and the shear homogenization is performed using a shearing machine; the microfluidization homogenization processing parameters are: single 19000-20000 psi.

[0025] The present invention also relates to the use of the above composition or the composition prepared by the above preparation method in the preparation of cosmetics.

[0026] The present invention also relates to a cosmetic comprising the above composition or the composition prepared by the above preparation method.

[0027] Preferably, the cosmetic further comprises at least one of a preservative, an antioxidant and a fragrance.

[0028] Preferably, the cosmetic is toner, lotion, essence, cream, ointment, facial mask or freeze-dried powder.

[0029] Preferably, the composition is added to the cosmetic in an amount of 0.01%-10% by weight.

[0030] The beneficial effects of the present invention are: The antioxidant composition of the present invention uses carnosine, hydroxypinacolone retinoic acid ester and dimethylmethoxychromanol for compounding, which has multi-directional and multi-target effects, significant synergistic effects, significantly improved antioxidant and anti-aging effects, and low irritation, avoiding adverse reactions. In particular, when the three are in a specific ratio, the antioxidant and anti-aging effects are further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is the ROS signal intensity diagram of Example 1.

[0032] Figure 2 This is the ROS signal intensity diagram of Comparative Example 1.

[0033] Figure 3 This is the ROS signal intensity diagram of Comparative Example 2.

[0034] Figure 4 This is the ROS signal intensity diagram of Comparative Example 3. DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with specific embodiments, and the advantages and features of the present invention will become clearer as the description proceeds. However, these embodiments are exemplary only and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

[0036] Unless otherwise specified, all raw materials in the present invention are conventional commercially available products.

[0037] In each embodiment of the present invention, carnosine was purchased from Dickman Biotechnology Co., Ltd., with a catalog number of 003150-00395-5592; grape seed oil was purchased from Zhongyi Biotechnology Co., Ltd., with a catalog number of 005161-03709-8693; rice bran oil was purchased from Tsuno Food Industrial Co., Ltd., with a catalog number of 001875-04642-4626; and meadowfoam seed oil was purchased from Henry Lamotte Oils GmbH, with a catalog number of 001103-01898-4525.

[0038] Example 1 A high-efficiency, low-irritation peptide-containing antioxidant composition comprises the following raw materials in percentage by weight: 2% carnosine, 0.2% hydroxypinacolone retinoic acid ester, 4% dimethylmethoxychromanol, 2% hydrogenated lecithin, 1% ceteareth-12, 2% grape seed oil, 2% rice bran oil, 5% meadowfoam seed oil, 25% glycerin and the balance of pure water.

[0039] The preparation method specifically comprises the following steps: S1: Carnosine, dimethylmethoxychromanol, hydrogenated lecithin, glycerol and the remainder of pure water are mixed and stirred at a rate of 800 rpm at room temperature to obtain system 1; S2: mixing and stirring hydroxypinacolone retinoic acid ester, ceteareth-12, grape seed oil, rice bran oil, and meadowfoam seed oil at a heating rate of 800 rpm at 50° C. to obtain system 2; S3: System 1 (aqueous phase) and system 2 (oil phase) are uniformly mixed in a temperature-controlled reactor at 50°C with stirring at 600rpm to obtain a peptide antioxidant composition mixture; the peptide antioxidant composition mixture is subjected to a mixing and shearing homogenization treatment at 14000rpm at room temperature to obtain a mixed colostrum, and then the mixed colostrum is subjected to a single 20000psi microfluidization homogenization treatment, and finally formed to obtain a high-efficiency and low-irritation peptide antioxidant composition.

[0040] Example 2 The invention discloses a high-efficiency and low-irritation peptide-containing antioxidant composition. The raw materials are composed as follows according to weight percentage: 1% carnosine, 0.1% hydroxypinacolone retinoic acid ester, 4% dimethylmethoxychromanol, 1% hydrogenated lecithin, 0.2% Tween-80, 1% grape seed oil, 1% rice bran oil, 1% meadowfoam seed oil, 10% glycerol and the balance of pure water.

[0041] The preparation method specifically comprises the following steps: S1: Carnosine, dimethylmethoxychromanol, hydrogenated lecithin, glycerol and the remainder of pure water were mixed and stirred at a rate of 700 rpm at room temperature to obtain system 1; S2: mixing and stirring hydroxypinacolone retinoic acid ester, Tween-80, grape seed oil, rice bran oil, and meadowfoam seed oil at 40° C. and a speed of 700 rpm to dissolve the mixture, to obtain system 2; S3: System 1 (aqueous phase) and system 2 (oil phase) are uniformly mixed in a temperature-controlled reactor at 40°C with stirring at 500rpm to obtain a peptide antioxidant composition mixture; the peptide antioxidant composition mixture is subjected to a mixing and shearing homogenization treatment at 13500rpm at room temperature to obtain a mixed colostrum, and then the mixed colostrum is subjected to a single 19000psi microfluidization homogenization treatment, and finally formed to obtain a high-efficiency and low-irritation peptide antioxidant composition.

[0042] Example 3 The invention discloses a peptide-containing antioxidant composition with high efficiency and low stimulation. The raw materials are composed as follows according to weight percentage: 3% carnosine, 0.3% hydroxypinacolone retinoic acid ester, 12% dimethylmethoxychromanol, 3% hydrogenated lecithin, 2% PEG-8 stearate, 3% grape seed oil, 3% rice bran oil, 10% meadowfoam seed oil, 40% glycerol and the balance of pure water.

[0043] The preparation method specifically comprises the following steps: S1: Carnosine, dimethylmethoxychromanol, hydrogenated lecithin, glycerol and the remainder of pure water are mixed and stirred at a rate of 800 rpm at room temperature to obtain system 1; S2: mixing and stirring hydroxypinacolone retinoic acid ester, PEG-8 stearate, grape seed oil, rice bran oil, and meadowfoam seed oil at 50° C. at a rate of 800 rpm to dissolve, to obtain system 2; S3: System 1 (aqueous phase) and system 2 (oil phase) are uniformly mixed in a temperature-controlled reactor at 50°C with stirring at 600rpm to obtain a peptide antioxidant composition mixture; the peptide antioxidant composition mixture is subjected to a mixing and shearing homogenization treatment at 14000rpm at room temperature to obtain a mixed colostrum, and then the mixed colostrum is subjected to a single 20000psi microfluidization homogenization treatment, and finally formed to obtain a high-efficiency and low-irritation peptide antioxidant composition.

[0044] The difference between Comparative Examples 1 to Comparative Examples 3 and Example 1 is that the total amount of the following ingredients (see Table 1) remains unchanged, the amount of each component is different, and the other conditions are the same.

[0045] Table 1

[0046] Example 4-Example 5 Screening of raw material dosage ratio The difference between Example 4-5 and Example 1 is that the total amount of the following ingredients (see Table 2) remains unchanged, the dosage ratio is different, and the other conditions are the same, as follows: Table 2 Mass ratio of carnosine, hydroxypinacolone retinoic acid ester and dimethylmethoxychromanol Example 4 1:0.1:4 Example 5 1:0.1:6 Test Example 1 Antioxidant Test 54 48-hour-old zebrafish embryos were randomly divided into 9 groups, with 6 in each group. They were exposed to a sample solution of a peptide antioxidant composition (the peptide antioxidant composition prepared in each embodiment and comparative example) with a formula addition concentration of 1wt%, and a blank control group without any ingredients was set up. After 24 hours of exposure, the fish embryos were stained with H2DCFDA, and the ROS signal intensity was measured by fluorescence photography and statistical analysis was performed. The results are shown in Table 3. The ROS signal intensity of Example 1 and Comparative Examples 1-3 is shown in Table 3. Figure 1-4 shown.

[0047] Table 3 ROS clearance rate of each embodiment and comparative example

[0048] Note: Compared with the blank control group, ** p <0.01,*** p <0.001; Compared with Example 1, && p <0.01,&&& p <0.001.

[0049] Test Example 2 Collagen content test 1) Cell seeding: According to the appropriate seeding density (8×10 4 Collagen cells were seeded into 24-well plates and incubated overnight in an incubator (37°C, 5% CO2).

[0050] 2) Experimental grouping: The experiment set up blank control group, negative control group, positive control group and sample group.

[0051] 3) Administration: Administration was performed when the cell plating rate in the 24-well plate reached 40%-60%. 1 mL of cell culture medium was added to each well of the blank control group and the negative control group; 1 mL of culture medium containing 100 μg / mL vitamin C and 7 pg / mL vitamin E was added to each well of the positive control group; 1 mL of culture medium containing the antioxidant composition of each embodiment or comparative example was added to each well of the sample group.

[0052] 4) Radiation: After administration, the negative control group, positive control group and sample group received UVA radiation with a total dose of 9 J / cm². At the same time, the blank control group was placed in the same environment (UVA radiation dose of 0 J / cm²).

[0053] 5) Collecting cell supernatant: After incubation for 24 hours, collect the cell culture supernatant into an EP tube and store it in a -80°C refrigerator.

[0054] 6) Detection of collagen I content: The content of type I collagen (Collagen I) was detected and analyzed according to the operating instructions of the ELISA detection kit. The results are shown in Table 4.

[0055] Table 4 Type I collagen content test of each embodiment and comparative example

[0056] Test Example 3 Skin irritation test The human skin patch test was conducted with reference to the "Hygienic Standards for Cosmetics". The results showed that the antioxidant compositions of Examples 1-5 of the present invention were safe and non-irritating, indicating that the compositions of the present invention are less irritating when used in cosmetics and avoid adverse reactions.

[0057] The above detailed description is a specific description of one feasible embodiment of the present invention. The embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not deviate from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A peptide-containing antioxidant composition, characterized in that: The composition comprises the following components by weight: 1-3 parts of carnosine, 0.1-0.3 parts of hydroxypinacolone retinoic acid ester and 4-12 parts of dimethylmethoxychromanol; The composition also includes at least one of a phospholipid emulsifier, a nonionic co-emulsifier, grape seed oil, rice bran oil, white meadowfoam seed oil, glycerin and water; the phospholipid emulsifier includes hydrogenated lecithin; the nonionic co-emulsifier includes at least one of Tween-80, laureth-4, ceteareth-12, PEG-8 stearate and glyceryl isostearate.

2. The composition according to claim 1, characterized in that The mass ratio of the carnosine, hydroxypinacolone retinoic acid ester and dimethylmethoxychromanol is 1:0.1:4-6.

3. The composition according to claim 1 or 2, characterized in that The composition comprises, by weight percentage, 1-3% of carnosine, 0.1-0.3% of hydroxypinacolone retinoic acid ester, 4-12% of dimethylmethoxychromanol, 1-3% of phospholipid emulsifier, 0.2-2% of nonionic auxiliary emulsifier, 1-3% of grape seed oil, 1-3% of rice bran oil, 1-10% of meadowfoam seed oil, 10-40% of glycerol and the balance of water.

4. A method for preparing the composition according to claim 3, characterized in that: The steps include: (1) mixing carnosine, dimethylmethoxychromanol, phospholipid emulsifier, glycerol and water to obtain an aqueous phase mixture; (2) mixing hydroxypinacolone retinoic acid ester, nonionic emulsifier, grape seed oil, rice bran oil, and meadowfoam seed oil to obtain an oil phase mixture; (3) mixing the aqueous phase mixture and the oil phase mixture, heating and stirring to obtain a premix; (4) The premix is ​​subjected to shear homogenization to obtain colostrum, and then subjected to microfluidization homogenization to obtain colostrum.

5. The preparation method according to claim 4, characterized in that: The stirring rate in step (1) is 700-800 rpm; the stirring rate in step (2) is 700-800 rpm, and the stirring temperature is 40-50°C; the heating temperature in step (3) is 40-50°C, and the stirring rate is 500-600 rpm; the shear homogenization rate in step (4) is 13500-14000 rpm, and the microfluidization homogenization processing parameters are: single 19000-20000 psi.

6. A cosmetic, characterized in that: The invention comprises the composition described in any one of claims 1 to 3 or the composition prepared by the preparation method described in any one of claims 4 to 5.

7. The cosmetic according to claim 6, characterized in that: It also includes at least one of a preservative, an antioxidant and a flavor.

8. The cosmetic according to claim 6 or 7, characterized in that: The cosmetic is toner, lotion, essence, cream, ointment, facial mask or freeze-dried powder; and / or In terms of weight percentage, the composition is added to the cosmetic in an amount of 0.01%-10%.

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