Functional soil conditioner

Abstract

The invention belongs to the technical field of soil conditioners, and particularly relates to a functional soil conditioner which comprises a bactericidal active component for killing or inhibiting pathogenic bacteria of fungal soil-borne diseases, the bactericidal active component is prepared by compounding L-alkannin, fenaminstrobin, difenoconazole, pydiflumetofen or coumoxystrobin according to a mass ratio of (1 to 80): (80 to 1). The functional soil conditioner disclosed by the invention contains the potassium humate and the bactericidal active component, the potassium humate can condition and improve soil, and the bactericidal active component can effectively kill or inhibit fungal soil-borne disease pathogenic bacteria. Compared with single use of potassium humate, the function of the soil conditioner can be enriched. Besides, the bactericidal active component is formed by compounding two bactericidal components, and shows a combined synergistic effect within a certain mass ratio range, so that the control effect on targets can be improved, accumulation and development of drug resistance of pathogenic bacteria can be delayed, and meanwhile, comprehensive utilization of existing bactericidal chemical agents can also be realized.

Description

Technical Field

[0001] The invention belongs to the technical field of soil conditioners, and in particular relates to a functional soil conditioner. Background Art

[0002] Potassium humate is a highly efficient organic potassium fertilizer that can increase the available potassium content in the soil, reduce potassium loss, and increase the absorption and utilization of potassium by crops. In addition, potassium humate can improve the physical characteristics of the soil and improve the soil aggregate structure; increase the soil's cation exchange capacity, fertilizer retention capacity, and adsorption and exchange of plant nutrients, and improve the slow release of fertilizers; directly affect cell metabolism, improve crop respiration and photosynthesis, and enhance crop resistance, such as drought resistance, cold resistance, and disease resistance. Although potassium humate has the above functions when used as a soil conditioner, in many cases it only improves the physical or chemical properties and activity of the soil, and does not have the function of killing or inhibiting pathogens that infect crops in the soil.

[0003] In the process of planting and growing crops, they will inevitably be harmed by pests and diseases. At present, the diseases on crops are mainly controlled by applying chemical agents. Chemical agents have the advantages of quick effect, target standard and convenient application, but with the long-term unscientific use of chemical agents, such as the long-term use of single-ingredient chemical agents, the resistance of pathogens has developed and accumulated rapidly, resulting in the prevention and control effect of chemical agents decreasing year by year, or even becoming ineffective. Although the development of new compounds can effectively solve the above problems, it has a long cycle and high cost, and it will also waste existing chemical agents. Compounding and screening different bactericidal active ingredients can, to a certain extent, not only improve the prevention and control effect of diseases, but also make comprehensive use of existing chemical agents, which is a convenient and effective way.

[0004] However, in the actual compounding screening process, not any two or more chemical agents can be compounded to achieve a combined synergistic effect. Moreover, there is no necessary relationship between the combined synergistic effect of pesticide compounding and the structure and activity of similar compounds. This can also be verified in the prior art. For example, the patent CN113841707B discloses the use of trans-farnesol as a synergist in the prevention and treatment of litchi downy mildew. In its public specification, farnesol and trans-farnesol are isomers of each other, and the EC of both for litchi downy mildew is 2.34. 50 In the mass ratio of 1-32:1, the SR of the combination of azoxystrobin and farnesol to litchi downy mildew was 0.56-0.91, both showing an additive effect; but the SR of the combination of azoxystrobin and trans-farnesol to litchi downy mildew was 5.9-9.0, both showing a synergistic effect.

[0005] The inventors found through the indoor mycelium growth rate method that within a certain mass ratio range, when levorotatory shikonin is compounded with enoxamyl, difenoconazole, fluazifop-butyl or syringoxystrobin, it exhibits a combined synergistic effect on Rhizoctonia solani, which causes rice sheath blight. Based on this, the control effect of rice sheath blight can be improved.

[0006] So far, there are no reports on the combination of L-shikonin with enoxamyl, difenoconazole, fluopicolide or syringoxystrobin.

[0007] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0008] The purpose of the present invention is to provide a functional soil conditioner, which can enrich the function of the soil conditioner compared with the use of potassium humate alone; compared with the application of a single chemical agent, it can improve the prevention and control effect of diseases and delay the accumulation of drug resistance of pathogens.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A functional soil conditioner comprises a fungicidal active ingredient for killing or inhibiting pathogens of fungal soil-borne diseases, wherein the fungicidal active ingredient is compounded by levorotatory shikonin and enoxamyl, difenoconazole, fluopicolide or syringoxystrobin in a mass ratio of 1-80:80-1.

[0011] Preferably, the mass ratio of the L-shikonin to enoxapeptide is 1-20:3-1.

[0012] Preferably, the mass ratio of the L-shikonin to difenoconazole is 1-8:12-1.

[0013] Preferably, the mass ratio of the levorotatory shikonin to the fluopicolide is 1-30:20-1.

[0014] Preferably, the mass ratio of the levorotatory shikonin to syringoxystrobin is 1-10:15-1.

[0015] Preferably, the functional soil conditioner is composed of the following ingredients, measured by mass percentage: 0.75-6% bactericidal active ingredient, 30-50% rice husk biochar, 8-12% alginate type mineral source potassium humate and the remainder of inert filler.

[0016] Preferably, the preparation method of the rice husk biochar is: take fresh rice husk, wash and dry it to constant weight, pyrolyze it in a pyrolysis furnace at 500°C for 6 hours without oxygen, cool it to room temperature naturally, rinse it with clean water, dry it to constant weight, and crush it through a 150-mesh sieve to obtain the rice husk biochar.

[0017] As a preferred method, the alginate-based mineral-source potassium humate was purchased from Yantai Jiatian Biotechnology Co., Ltd. 2 O 5 +K 2 O ≥ 5%, organic matter ≥ 45%, mineral humic acid ≥ 60%, water solubility ≥ 99%.

[0018] Preferably, the inert filler is selected from one or more of light calcium carbonate, bentonite, attapulgite, diatomaceous earth, white carbon black and kaolin.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The functional soil conditioner of the present invention contains potassium humate and bactericidal active ingredients, potassium humate can condition and improve the soil, and the bactericidal active ingredients can effectively kill or inhibit fungal soil-borne pathogens. Compared with the use of potassium humate alone, the function of the soil conditioner can be enriched. In addition, the bactericidal active ingredient is compounded by two bactericidal ingredients, and exhibits a combined synergistic effect within a certain mass ratio range, which can improve the prevention and control effect on the target, delay the accumulation and development of pathogen resistance, and also realize the comprehensive utilization of existing bactericidal chemical agents. DETAILED DESCRIPTION

[0021] Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0022] 1. Materials and Methods

[0023] 1.1 Test strains

[0024] Rhizoctonia solani was isolated from diseased rice plants in the rice experimental field of Guangxi Zhuang Autonomous Region Academy of Agricultural Sciences and preserved on PDA medium.

[0025] 1.2 Test culture medium

[0026] PDA culture medium formula: 200g peeled potatoes, 20g glucose, 15g agar, 1000L water, natural pH.

[0027] 1.3 Test agents

[0028] 96% L-shikonin technical (CAS: 517-88-4, Shanghai Aladdin Biochemical Technology Co., Ltd.), 98% oxadiazine technical (Shenyang Kechuang Chemical Co., Ltd.), 97% difenoconazole technical (Hunan Bede Biochemical Technology Co., Ltd.), 98% fluazifop-butyl hydroxylamide (Jiangsu Youjia Plant Protection Co., Ltd.), 96% syringoxystrobin technical (Jilin Bada Pesticide Co., Ltd.)

[0029] 1.4 Test methods

[0030] The mycelium growth rate method was used to determine the toxicity of the tested single agents and their mixed agents to the rice sheath blight pathogen Rhizoctonia solani, and then the Sun Yunpei co-toxicity coefficient method was used to evaluate the type of joint action of the mixed agents.

[0031] The test agent was dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to form a single-dose mother solution, and the concentration of the active ingredient in the single-dose mother solution was 10000 mg / L. Then multiple groups of proportions were set up, and each single-dose mother solution and each group of proportioned mixtures were diluted with 0.1% Tween-80 aqueous solution to form 5 mass concentration gradients.

[0032] Melt the PDA medium in advance under sterile conditions, and when it cools to about 55°C, mix the PDA medium and the drug solution in a volume ratio of 9:1, shake evenly, and pour into a 9cm diameter culture dish, 10mL per culture dish, and make a drug-containing plate after complete cooling, and set the treatment of 0.1% Tween-80 aqueous solution as a blank control. Repeat 10 plates for each mass concentration of drug solution treatment and blank control treatment.

[0033] The test strain was inoculated on PDA medium and cultured in a 25℃ constant temperature incubator for 3 days. Then, a 5mm diameter bacterial cake was cut from the edge of the colony with a hole puncher and inoculated in the center of the plate. One bacterial cake was inoculated per dish, with the mycelium facing down and close to the PDA medium. After the dish was covered, it was placed in a 25℃ constant temperature incubator for further culture. When the diameter of the blank control colony grew to 6-7cm, the colony diameter was measured by the cross method, and the inhibition rate of mycelium growth by different treatments was calculated.

[0034]

[0035] 2. Data processing

[0036] DPS software was used for data statistical analysis. The logarithmic value of fungicide concentration was taken as x and the corresponding probability value of mycelium growth inhibition rate was taken as y for linear regression. The toxicity regression equation and the toxicity EC value of the agent to the target pathogen were obtained. 50 The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0037] 3. Evaluation of joint effects

[0038] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC), CTC ≤ 80 for antagonistic effect, 80 < CTC < 120 for additive effect, and CTC ≥ 120 for synergistic effect. The results are shown in Tables 1-4.

[0039] Table 1 The results of toxicity test of the combination of L-shikonin and enoxamyl against Rhizoctonia solani

[0040]

[0041] As shown in Table 1, within the mass ratio range of 1-20:3-1, the co-toxicity coefficient of the combination of levorotatory shikonin and enoxamyl to Rhizoctonia solani was greater than 120, showing a synergistic effect.

[0042] Table 2 The results of toxicity test of the combination of L-shikonin and difenoconazole against Rhizoctonia solani

[0043]

[0044] As shown in Table 2, within the mass ratio range of 1-8:12-1, the co-toxicity coefficient of the combination of levorotatory shikonin and difenoconazole against Rhizoctonia solani was greater than 120, showing a synergistic effect.

[0045] Table 3 The results of toxicity test of the combination of levorotatory shikonin and fluopicolide against Rhizoctonia solani

[0046]

[0047] As shown in Table 3, within the mass ratio range of 1-30:20-1, the co-toxicity coefficient of the combination of levorotatory shikonin and fluopicolide against Rhizoctonia solani was greater than 120, showing a synergistic effect.

[0048] Table 4 The results of the toxicity test of the combination of L-shikonin and syringoxystrobin against Rhizoctonia solani

[0049]

[0050] As shown in Table 4, within the mass ratio range of 1-10:15-1, the co-toxicity coefficient of the combination of levorotatory shikonin and syringoxystrobin against Rhizoctonia solani was greater than 120, showing a synergistic effect.

[0051] Functional soil conditioner 1

[0052] The functional soil conditioner is composed of the following ingredients in percentage by mass: 1.2% of fungicide active ingredient, 30-50% of rice husk biochar, 8-12% of alginate-type mineral-source potassium humate and the balance of bentonite; wherein the fungicide active ingredient is compounded by L-shikonin and enoxamyl in a mass ratio of 5:1;

[0053] The preparation method of the rice husk biochar is as follows: fresh rice husk is taken, washed and dried to constant weight, pyrolyzed in a pyrolysis furnace at 500°C for 6 hours without oxygen, naturally cooled to room temperature, rinsed with clean water, dried to constant weight, and crushed through a 150-mesh sieve to obtain the rice husk biochar; the alginate-based mineral-source potassium humate was purchased from Yantai Jiatian Biotechnology Co., Ltd., N+P 2 O 5 +K 2 O ≥ 5%, organic matter ≥ 45%, mineral humic acid ≥ 60%, water solubility ≥ 99%.

[0054] Functional soil conditioner 2

[0055] The functional soil conditioner is composed of the following ingredients in percentage by mass: 1.2% of fungicide active ingredient, 30-50% of rice husk biochar, 8-12% of alginate-type mineral-source potassium humate and the balance of bentonite; wherein the fungicide active ingredient is compounded by L-shikonin and difenoconazole in a mass ratio of 1:4;

[0056] The preparation method of the rice husk biochar is as follows: fresh rice husk is taken, washed and dried to constant weight, pyrolyzed in a pyrolysis furnace at 500°C for 6 hours without oxygen, naturally cooled to room temperature, rinsed with clean water, dried to constant weight, and crushed through a 150-mesh sieve to obtain the rice husk biochar; the alginate-based mineral-source potassium humate was purchased from Yantai Jiatian Biotechnology Co., Ltd., N+P 2 O 5 +K 2 O ≥ 5%, organic matter ≥ 45%, mineral humic acid ≥ 60%, water solubility ≥ 99%.

[0057] Functional soil conditioner 3

[0058] The functional soil conditioner is composed of the following ingredients in percentage by mass: 1.2% of fungicide active ingredient, 30-50% of rice husk biochar, 8-12% of alginate-type mineral-source potassium humate, and the balance of bentonite; wherein the fungicide active ingredient is compounded by L-shikonin and fluopicolide at a mass ratio of 1:5;

[0059] The preparation method of the rice husk biochar is as follows: fresh rice husk is taken, washed and dried to constant weight, pyrolyzed in a pyrolysis furnace at 500°C for 6 hours without oxygen, naturally cooled to room temperature, rinsed with clean water, dried to constant weight, and crushed through a 150-mesh sieve to obtain the rice husk biochar; the alginate-based mineral-source potassium humate was purchased from Yantai Jiatian Biotechnology Co., Ltd., N+P 2 O 5 +K 2 O ≥ 5%, organic matter ≥ 45%, mineral humic acid ≥ 60%, water solubility ≥ 99%.

[0060] Functional soil conditioner 4

[0061] The functional soil conditioner is composed of the following ingredients in percentage by mass: 1.2% of bactericidal active ingredient, 30-50% of rice husk biochar, 8-12% of alginate-type mineral-source potassium humate and the balance of bentonite; wherein the bactericidal active ingredient is compounded by L-shikonin and syringoxystrobin in a mass ratio of 1:10;

[0062] The preparation method of the rice husk biochar is as follows: fresh rice husk is taken, washed and dried to constant weight, pyrolyzed in a pyrolysis furnace at 500°C for 6 hours without oxygen, naturally cooled to room temperature, rinsed with clean water, dried to constant weight, and crushed through a 150-mesh sieve to obtain the rice husk biochar; the alginate-based mineral-source potassium humate was purchased from Yantai Jiatian Biotechnology Co., Ltd., N+P 2 O 5 +K 2 O ≥ 5%, organic matter ≥ 45%, mineral humic acid ≥ 60%, water solubility ≥ 99%.

[0063] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A functional soil conditioner, comprising a fungicidal active ingredient for killing or inhibiting pathogens of fungal soil-borne diseases, characterized in that: The bactericidal active ingredient is prepared by compounding levorotatory shikonin with enoxamyl, difenoconazole, fluopicolide or syringoxystrobin in a mass ratio of 1-80:80-1.

2. The functional soil conditioner according to claim 1, characterized in that The mass ratio of the levorotatory shikonin to enoxamil is 1-20:3-1.

3. The functional soil conditioner according to claim 1, characterized in that The mass ratio of the L-shikonin to difenoconazole is 1-8:12-1.

4. The functional soil conditioner according to claim 1, characterized in that The mass ratio of the levorotatory shikonin to the fluopicolide is 1-30:20-1.

5. The functional soil conditioner according to claim 1, characterized in that: The mass ratio of the levorotatory shikonin to syringoxystrobin is 1-10:15-1.

6. The functional soil conditioner according to claim 1, characterized in that: The functional soil conditioner consists of the following components in percentage by mass: 0.75-6% of bactericidal active ingredients, 30-50% of rice husk biochar, 8-12% of alginate-type mineral-source potassium humate and the remainder of inert filler.

7. The functional soil conditioner according to claim 6, characterized in that The preparation method of the rice husk biochar is as follows: fresh rice husk is taken, washed and dried to constant weight, pyrolyzed in a pyrolysis furnace at 500° C. for 6 hours without oxygen, naturally cooled to room temperature, rinsed with clean water, dried to constant weight, and crushed through a 150-mesh sieve to obtain the rice husk biochar.

8. The functional soil conditioner according to claim 6, characterized in that: The alginate-type mineral-source potassium humate was purchased from Yantai Jiatian Biotechnology Co., Ltd., with N+P2O5+K2O≥5%, organic matter≥45%, mineral-source humic acid≥60%, and water solubility≥99%.

9. The functional soil conditioner according to claim 6, characterized in that: The inert filler is selected from one or more of light calcium carbonate, bentonite, attapulgite, diatomaceous earth, white carbon black and kaolin.

Images