Anti-aging skin care product containing taurine and preparation method thereof

Abstract

According to the taurine-containing anti-aging skin care product and the preparation method thereof disclosed by the invention, taurine, sodium hyaluronate and gamma-aminobutyric acid are compounded to prepare nano-emulsion, and then the nano-emulsion is added into the skin care product, so that the stability and permeability of taurine are effectively improved; meanwhile, through nanocrystallization treatment, the permeation effect of the skin care product is also effectively improved, and the anti-aging effect is further improved.

Description

Technical Field

[0001] The invention belongs to the field of skin care products, and particularly relates to an anti-aging skin care product containing taurine and a preparation method thereof. Background Art

[0002] As we age, our skin gradually shows signs of aging, such as wrinkles and sagging. 80% of the causes of skin aging are caused by free radicals. Free radicals attack the skin cell membrane, destroying its elasticity and flexibility, leading to microcirculation disorders, decreased skin cell immune function, and loss of nutrition for the skin, which in turn leads to skin aging, wrinkling, loss of elasticity and luster. In addition, the deposition of free radicals can also lead to skin metabolism disorders, melanin deposition, and cause skin dullness and spots.

[0003] As people's pursuit of beauty increases, the market demand for anti-aging skin care products continues to expand. As an ingredient with anti-aging effects, taurine has gradually attracted attention in cosmetics. As a natural amino acid, taurine has antioxidant, anti-inflammatory, skin repair and sun protection effects, and can effectively fight skin aging. However, the traditional method for preparing taurine skin care products usually directly adds taurine to the skin care product matrix, lacks the step of optimizing the properties of taurine, thus affecting its anti-aging effect. Summary of the invention

[0004] The purpose of the present invention is to provide an anti-aging skin care product containing taurine and a preparation method thereof, so as to solve the problem that traditional taurine skin care products have weak anti-aging effects and improve stability and permeability.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions: A taurine-containing nanoemulsion comprises the following components in parts by weight: 1-10 parts of taurine, 1-10 parts of sodium hyaluronate, 1-10 parts of gamma-aminobutyric acid, 15-40 parts of emulsifier, 1-10 parts of co-emulsifier, 1-10 parts of liquid lipids and 10-40 parts of aqueous phase.

[0006] Furthermore, the emulsifier includes one or more of poloxamer, polyoxyethylene castor oil emulsifier, shea butter, squalane oil, and polyglycerol emulsifier.

[0007] Furthermore, the co-emulsifier includes one or more of laureth-23, diethylene glycol monoethyl ether, PPG-26-butanol polyether-26, tridecyl alcohol polyether-12, and hydrogenated castor oil polyoxyethylene ether RH40.

[0008] Furthermore, the liquid lipid includes one or more of isopropyl myristate, isopropyl palmitate, caprylic / capric triglyceride, and caprylic triglyceride.

[0009] The method for preparing the nanoemulsion is characterized in that it comprises the following steps: (1) stirring taurine, sodium hyaluronate, γ-aminobutyric acid and liquid lipid at 300-400 rpm and 40° C. to obtain a first mixed solution; (2) adding the emulsifier and the co-emulsifier to the first mixed solution, and stirring at 350-400 rpm to obtain a second mixed solution; (3) adding water to the second mixed solution and stirring at 350-400 rpm to obtain a third mixed solution; (4) The third mixed solution is homogenized in a high-pressure homogenizer at 900-1000 bar to obtain a nanoemulsion.

[0010] Furthermore, the particle size of the nanoemulsion is 25-45 nm.

[0011] A skin care product comprising the nanoemulsion according to any one of claims 1 to 6.

[0012] Furthermore, the content of the nanoemulsion in the skin care product is 1-3%.

[0013] Furthermore, the skin care product is one of toner, essence, cream and lotion.

[0014] The present invention directly uses high-purity taurine as a raw material. Taurine exerts anti-aging effects on the skin through multiple mechanisms, including regulating cell osmotic balance, protecting the skin from UVB radiation damage, affecting the health of the epidermis and dermis, anti-oxidation, moisturizing and repairing the skin barrier, etc. These effects work together on the skin, helping to slow down the skin aging process and keep the skin healthy and youthful.

[0015] Sodium hyaluronate can increase the elasticity and firmness of the skin, fill fine lines and wrinkles, and make the skin appear smoother and younger. Small molecule hyaluronic acid can penetrate into the dermis, promote blood microcirculation, and facilitate the skin's absorption of nutrients, thus playing a role in beauty and anti-wrinkle.

[0016] GABA's unique calming and soothing effect can regulate skin sensitivity and reduce discomfort caused by external stimuli. In addition, it can also serve as a powerful antioxidant to resist free radical damage, slow down skin aging, and keep the skin young and elastic.

[0017] The present invention utilizes the properties of sodium hyaluronate and γ-aminobutyric acid, and by compounding them with taurine and then using the form of nanoemulsion, not only can the penetration effect of taurine be further enhanced, but taurine, in collaboration with sodium hyaluronate and γ-aminobutyric acid, can also further enhance the anti-aging effects of each component.

[0018] The beneficial effects of the present invention are: by optimizing the formula and preparation process, the stability and permeability of taurine in skin care products are improved, so as to give full play to its anti-aging effect. At the same time, the product has a better use experience and can meet the needs of consumers for anti-aging skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 : The taurine penetration of Application Example 1 and Comparative Example 1 and Comparative Example 4 on pig skin at 12 and 24 hours. DETAILED DESCRIPTION

[0020] The technical solution of the present invention is further described in detail below through specific embodiments. It should be understood that the implementation of the present invention is not limited to the following embodiments, and any form of modification and / or change made to the present invention will fall within the protection scope of the present invention.

[0021] The materials and instruments used in the following examples can all be purchased from commercial sources.

[0022] Example 1 The components and contents of the present embodiment are: 3 parts of taurine, 3 parts of sodium hyaluronate, 3 parts of γ-aminobutyric acid, 6 parts of caprylic triglyceride, 15 parts of shea butter, 5 parts of laureth-23, and 65 parts of water.

[0023] The specific preparation steps are: (1) Stir 3 parts of taurine, 3 parts of sodium hyaluronate, 3 parts of γ-aminobutyric acid and 6 parts of caprylic triglyceride at 300 rpm and 40°C until uniform; (2) adding 15 parts of shea butter and 5 parts of laureth-23 and stirring at 350 rpm and 40° C. for 20 min; (3) Add 65 parts of water to the mixed solution obtained in step (2), and stir at 400 rpm and 40°C for 20 min; (4) The mixed solution obtained in step (3) was homogenized in a 1000 bar high-pressure homogenizer to obtain a taurine-containing nanoemulsion with an average particle size of 35.01 nm.

[0024] Example 2 The components and contents of the present embodiment are: 5 parts of taurine, 5 parts of sodium hyaluronate, 5 parts of gamma-aminobutyric acid, 10 parts of caprylic triglyceride, 20 parts of shea butter, 5 parts of laureth-23, and 50 parts of water.

[0025] The specific preparation steps are: (1) Stir 5 parts of taurine, 5 parts of sodium hyaluronate, 5 parts of γ-aminobutyric acid and 10 parts of caprylic triglyceride at 300 rpm and 40°C until uniform; (2) adding 20 parts of shea butter and 5 parts of laureth-23 and stirring at 350 rpm and 40°C for 20 minutes; (3) Add 50 parts of water to the mixed solution obtained in step (2), and stir at 400 rpm and 40°C for 20 min; (4) The mixed solution obtained in step (3) was homogenized in a 1000 bar high-pressure homogenizer to obtain a taurine-containing nanoemulsion with an average particle size of 37.40 nm.

[0026] Example 3 The components and contents of this embodiment are: 5 parts of taurine, 5 parts of sodium hyaluronate, 5 parts of γ-aminobutyric acid, 10 parts of isopropyl myristate, 20 parts of squalane oil, 5 parts of hydrogenated castor oil polyoxyethylene ether RH40, and 50 parts of water.

[0027] The specific preparation steps are: (1) Stir 5 parts of taurine, 5 parts of sodium hyaluronate, 5 parts of γ-aminobutyric acid and 10 parts of isopropyl myristate at 300 rpm and 40°C until uniform; (2) Add 20 parts of squalane oil and 5 parts of hydrogenated castor oil polyoxyethylene ether RH40 and stir at 350 rpm and 40°C for 20 minutes; (3) Add 50 parts of water to the mixed solution obtained in step (2), and stir at 400 rpm and 40°C for 20 min; (4) The mixed solution obtained in step (3) was homogenized in a 1000 bar high-pressure homogenizer to obtain a taurine-containing nanoemulsion with an average particle size of 36.28 nm.

[0028] Example 4 The components and contents of this embodiment are: 10 parts of taurine, 5 parts of sodium hyaluronate, 5 parts of γ-aminobutyric acid, 15 parts of isopropyl myristate, 25 parts of squalane oil, 10 parts of hydrogenated castor oil polyoxyethylene ether RH40, and 30 parts of water.

[0029] (1) Stir 10 parts of taurine, 5 parts of sodium hyaluronate, 5 parts of γ-aminobutyric acid and 15 parts of isopropyl myristate at 300 rpm and 40°C until uniform; (2) Add 25 parts of squalane oil and 10 parts of hydrogenated castor oil polyoxyethylene ether RH40 and stir at 350 rpm and 40°C for 20 minutes; (3) Add 30 parts of water to the mixed solution obtained in step (2), and stir at 400 rpm and 40°C for 20 min; (4) The mixed solution obtained in step (3) was homogenized in a 1000 bar high-pressure homogenizer to obtain a taurine-containing nanoemulsion with an average particle size of 40.11 nm.

[0030] Comparative Example 1 The components and contents of this embodiment are: 9 parts of taurine, 6 parts of caprylic triglyceride, 15 parts of shea butter, 5 parts of laureth-23, and 65 parts of water. The synthesis steps are the same as those of Example 1.

[0031] Comparative Example 2 The components and contents of this embodiment are: 9 parts of sodium hyaluronate, 6 parts of caprylic triglyceride, 15 parts of shea butter, 5 parts of laureth-23, and 65 parts of water. The synthesis steps are the same as those of Example 1.

[0032] Comparative Example 3 The components and contents of this embodiment are: 9 parts of gamma-aminobutyric acid, 6 parts of caprylic triglyceride, 15 parts of shea butter, 5 parts of laureth-23, and 65 parts of water. The synthesis steps are the same as those of Example 1.

[0033] Comparative Example 4 The components and contents of the present embodiment are: 3 parts of taurine, 3 parts of sodium hyaluronate, 3 parts of γ-aminobutyric acid, 6 parts of caprylic triglyceride, 15 parts of shea butter, 5 parts of laureth-23, and 65 parts of water, which are directly mixed.

[0034] Stability test: The nanoemulsions prepared in Examples 1-4 and Comparative Examples 1-3 were placed in a closed container and placed at room temperature and 4° C. for 3 months. No stratification or precipitation occurred, and the particle size did not change significantly, indicating that the nanoemulsions obtained by the method had good stability.

[0035] Efficacy Test An efficacy test essence was prepared. The raw materials included component A, component B and water. Based on 100% by mass, component A included: 5% dipropylene glycol, 10% glycerol, 15% propylene glycol, and 0.15% carbomer 941; component B included: 0.15% triethanolamine (TEA), 0.5% DM928 (preservative), and the balance was water.

[0036] The preparation method of the essence is: S1: Add the raw materials of component A to water, stir and dissolve at 35-45°C and 400-500rpm until a clear, uniform liquid without agglomeration is obtained to obtain phase A; S2: Add component B to phase A while stirring at 400-500 rpm, and slowly stir at 200-300 rpm until the liquid is uniform, to obtain a blank essence solution with a pH in the range of 5-7, and prepare each group of test samples according to the table below.

[0037] Table 1 Composition of each group of efficacy test samples

[0038] In 2022, a human patch test was conducted in accordance with the requirements of the "Technical Specifications for Safety of Cosmetics". A total of 120 subjects who met the test criteria were screened. Their ages ranged from 20 to 55 years old and they were randomly divided into 4 groups. The observation results were recorded at 30 minutes, 24 hours, and 48 hours. The results are shown in Table 2.

[0039] Table 2 Human patch test results Note: 0 indicates a negative reaction.

[0040] It can be seen from Table 2 that the essences obtained from Application Examples 1-4 and Comparative Examples 1-4 of the present invention are non-irritating to the skin and are safe to use.

[0041] (1) Transdermal absorption experiment The Franz diffusion cell method (transdermal diffusion experiment) was used to examine the 12 and 24 h permeation (%) of Application Example 1 and Application Comparative Examples 1 and 4 on pig skin. 0.5 g of the sample of Application Example 1 and Application Comparative Examples 1 and 4 was evenly applied on the surface of pig skin. The retention of taurine in the skin was determined by HPLC. The results are shown in Table 1. Figure 1 The penetration effect of taurine, sodium hyaluronate and γ-aminobutyric acid combined is significantly better than that of taurine alone, and compared with the free components, the penetration effect is also significantly improved by forming a nanoemulsion.

[0042] (2) In vitro antioxidant test 0.5 g of the essence of application examples 1-4 and comparative application examples 1-4 was dissolved in 100 mL of ethanol, and the DPPH free radical was determined by referring to [Tan Yao, Chen Ping, Xu Saihui, et al. Determination of the ability of homemade queen larvae whitening, anti-aging and anti-wrinkle cream to scavenge DPPH free radicals by DPPH method [J]. Guangdong Chemical Industry. 2019, 46 (18): 143-144]. The results are shown in Table 3.

[0043] Table 3 Results of in vitro antioxidant test

[0044] As can be seen from Table 3, the application examples 1-4 have good free radical scavenging ability, and the free radical scavenging ability of adding sodium hyaluronate and γ-aminobutyric acid to taurine is stronger than that of using the components alone.

[0045] (4) Anti-aging test 80 subjects were selected, with an average age of (35±3.2) years old; the volunteers were randomly divided into 8 groups, with 10 people in each group, and used the 8 essences in Table 1 respectively. The volunteers used the essences every morning and evening, and stopped using other skin care products. After using them for 4 consecutive weeks, the skin wrinkle improvement rate was measured by VISIA® instrument to evaluate the anti-wrinkle effect of the composition, and the stratum corneum water content was measured by Corneometer instrument to evaluate the moisturizing effect of the composition. The results are shown in Tables 4 and 5.

[0046] Table 4 Skin wrinkle improvement rate

[0047] As shown in Table 4, the wrinkle improvement effects of Examples 1-4 are better than those of Comparative Examples 1-4. Compared with the use of each component alone, the combination of the three can effectively improve the anti-aging effect; compared with the use of Comparative Example 4, the anti-aging effects of the three can be increased by first forming a nanoemulsion.

[0048] Table 5 Increase rate of stratum corneum water content

[0049] As shown in Table 5, the water content of the stratum corneum is significantly increased after using application examples 1-4, and the effect is better than using each component alone, and is also better than application comparative example 4 which is simply mixed.

[0050] In summary, sodium hyaluronate, as a widely recognized moisturizing ingredient, can penetrate deeply into the skin and improve the skin's absorption of other ingredients. And γ-aminobutyric acid can soothe nerve conduction, relax facial muscles, and reduce wrinkles. Therefore, the present invention synergistically increases the penetration of taurine in skin care products through the combination of sodium hyaluronate and γ-aminobutyric acid, and also improves its anti-aging effect through the interaction between the three. At the same time, adding the core component to the skin care product in the form of nanoemulsion can also further improve the anti-aging effect.

[0051] It is apparent to those skilled in the art that several improvements and modifications may be made to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A nanoemulsion containing taurine, characterized in that: The taurine-containing nanoemulsion comprises the following components in parts by weight: 1-10 parts of taurine, 1-10 parts of sodium hyaluronate, 1-10 parts of γ-aminobutyric acid, 15-40 parts of emulsifier, 1-10 parts of auxiliary emulsifier, 1-10 parts of liquid lipids, and 10-40 parts of aqueous phase.

2. The nanoemulsion according to claim 1, characterized in that: The emulsifier includes one or more of poloxamer, polyoxyethylene castor oil emulsifier, shea butter, squalane oil, and polyglycerol emulsifier.

3. The nanoemulsion according to claim 1, characterized in that: The auxiliary emulsifier includes one or more of laureth-23, diethylene glycol monoethyl ether, PPG-26-butanol polyether-26, trideceth-12, and hydrogenated castor oil polyoxyethylene ether RH40.

4. The nanoemulsion according to claim 1, characterized in that: The liquid lipids include one or more of isopropyl myristate, isopropyl palmitate, caprylic / capric triglyceride, and caprylic triglyceride.

5. A method for preparing a nanoemulsion according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) stirring taurine, sodium hyaluronate, γ-aminobutyric acid and liquid lipid at 300-400 rpm and 40° C. to obtain a first mixed solution; (2) adding the emulsifier and the co-emulsifier to the first mixed solution, and stirring at 350-400 rpm to obtain a second mixed solution; (3) adding water to the second mixed solution, and stirring at 350-400 rpm to obtain a third mixed solution; (4) The third mixed solution is homogenized in a high-pressure homogenizer at 900-1000 bar to obtain a nanoemulsion.

6. The preparation method according to claim 6, characterized in that: The particle size of the nanoemulsion is 25-45 nm.

7. A skin care product, characterized in that: The skin care product comprises the nanoemulsion according to any one of claims 1 to 6.

8. The skin care product according to claim 7, characterized in that: The content of the nanoemulsion in the skin care product is 1-3%.

9. The skin care product according to claim 8, characterized in that: The skin care product is one of toner, essence, cream and lotion.

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