PCR (Polymerase Chain Reaction) fluorescence signal detector based on MEMS (Micro Electro Mechanical System) technology

Abstract

The invention discloses a PCR fluorescence signal detector based on an MEMS technology, and relates to the technical field of nucleic acid detection.The PCR fluorescence signal detector comprises a machine body, a loading plate is installed at the bottom in an inner cavity of the machine body, and rapid clamping mechanisms used for guaranteeing fluorescence detection stability are installed on the left side and the right side of the upper end face of the loading plate correspondingly; a moving assembly is installed at the top of an inner cavity of the machine body, a wavelength-adjustable fluorescence emitter is fixedly installed on the moving assembly, the MEMS technology is used for adjusting voltage at the two ends of an electrode to generate drive to enable a film to deform, and therefore the adjustable spectrum filtering function and multi-band light filtering are achieved; therefore, a plurality of excitation optical filters needed by an original fluorescent channel can be effectively replaced, the optical sensor detection function is achieved, a CMOS camera in an imaging part in the original fluorescent channel can be replaced, one module replaces the principle optical filtering and receiving functions at the same time, the size is small, and the price is low.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a PCR fluorescence signal detector based on MEMS technology. Background Art

[0002] PCR, short for polymerase chain reaction, is a biotechnology that uses the principle of DNA double-strand replication to amplify specific nucleic acid samples in vitro. It can quickly copy trace amounts of nucleic acid samples to millions of times, making it easier for subsequent analysis and research. The technology originated in the 1970s and 1980s. After decades of development, it has evolved from the first generation of traditional PCR, the second generation of fluorescent quantitative PCR, and the third generation of digital PCR. It has been widely used in medical screening and diagnosis, basic research in life sciences, forensic analysis and identification, food hygiene, and environmental testing.

[0003] PCR detection uses fluorescence analysis, which refers to a method of qualitative or quantitative analysis that uses the fluorescence that can reflect the characteristics of a substance after certain substances are in an excited state after being irradiated with light. The excited state molecules undergo a collision and emission de-excitation process, and the fluorescence that occurs can reflect the characteristics of the substance. Since some substances do not emit fluorescence themselves (or the fluorescence is very weak), it is necessary to convert the non-fluorescent substances into substances that can emit fluorescence. For example, certain reagents (such as fluorescent dyes) are used to form complexes with non-fluorescent substances. Various complexes can emit fluorescence and then be measured. Therefore, the use of fluorescent reagents has opened the door to fluorescence analysis of some inorganic and organic substances that originally did not fluoresce, expanding the scope of analysis;

[0004] PCR optical detection system mainly relies on optical technology to detect nucleic acids, including lighting, imaging, filtering, lenses, detectors and other components, as well as different scanning methods to obtain information. PCR optical detection system is an indispensable part of the medical industry. Its core lies in capturing and analyzing signals during nucleic acid amplification through optical technology;

[0005] We found that traditional fluorescence detection mostly uses CCD (charge-coupled device) detectors, which may cause the test items to be insufficiently stable during detection and differences in optical path length, resulting in uneven distribution of light energy in multiple reaction wells, causing differences in the excitation light energy obtained by the fluorescent groups, ultimately affecting the accuracy of the experimental results. In addition, multiple filters are used, which is costly. Summary of the invention

[0006] The purpose of the present invention is to provide a PCR fluorescence signal detector based on MEMS technology to solve the problems raised in the above background technology.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a PCR fluorescence signal detector based on MEMS technology, comprising a body, a loading plate is installed at the bottom of the inner cavity of the body, and quick clamping mechanisms for ensuring the stability of fluorescence detection are installed on the left and right sides of the upper end surface of the loading plate, respectively; a moving component is installed at the top of the inner cavity of the body, and a wavelength-adjustable fluorescence emitter is fixedly installed on the moving component, and the fluorescence emitter comprises a silicon base, a thin film is arranged in the silicon base, a bottom electrode is deposited on the upper end surface of the silicon base, an upper glass plate is deposited on the upper end surface of the bottom electrode, a top electrode is deposited on the upper end surface of the upper glass plate, an external power supply is connected between the top electrode and the bottom electrode, a lower glass plate is fixedly installed at the bottom of the silicon base, and a light source is arranged on the lower end surface of the lower glass plate, and the deformation control of the thin film is realized by adjusting the voltage across the top electrode and the bottom electrode, and the wavelength of the light emitted by the light source is adjusted.

[0008] The top end face of said sliding panel also is provided with a toothed plate, and the toothed plate is meshed with toothed plates at the bottom.

[0009] Preferably, cylindrical portions are fixedly mounted on the extension portions on both sides of the top plate, support plates are slidably connected in the semicircular cavities on both sides of the opening groove, a compression spring is connected between the lower end surface of each hollow semi-cylinder and the support plate, and a trapezoidal protrusion is provided on each hollow semi-cylinder, and each cylindrical portion can abut against the corresponding trapezoidal protrusion.

[0010] Preferably, a base portion is provided at the bottom of the clamping plate body, and a mounting groove is provided on the upper end surface of the loading plate. The base portion is slidably connected in the mounting groove, and a lead screw is rotatably installed in the mounting groove. The lead screw passes through the base portion and is threadedly connected to the base portion, and a rotating disc is fixedly connected to the left end of the mounting groove.

[0011] Preferably, the moving component includes an electromagnetic slide rail driving seat fixedly mounted on the upper side wall of the inner cavity of the body, a connecting part is installed on the electromagnetic slide rail in the electromagnetic slide rail driving seat, another electromagnetic slide rail driving seat is installed on the connecting part, wherein a light source mounting plate is installed on the electromagnetic slide rail, and a fluorescent emitter is fixedly mounted on the lower end surface of the light source mounting plate.

[0012] Preferably, the front end surface of the loading plate is provided with a clamping plate portion on both sides thereof, and the lower end surface of the loading plate is provided with a sliding bar portion at each position of the clamping plate portion.

[0013] Preferably, a sinking cavity is provided at the bottom of the inner cavity of the machine body, an extension portion at the bottom of the rear end of the loading plate is slidably connected in the sinking cavity, and the slide portion is slidably connected in the bottom notch at the entrance of the front end face of the machine body.

[0014] Preferably, the bottom of the base portion is provided with protrusions on both sides, and the protrusions are respectively embedded in the slide grooves on both sides of the installation slide groove.

[0015] Preferably, a touch display screen is fixedly mounted on the upper end surface of the body.

[0016] In summary, the beneficial effects of the present invention are:

[0017] 1. The present invention utilizes MEMS technology to adjust the voltage at both ends of the electrode to generate a drive to deform the membrane, and adjusts the voltage to adjust the membrane deformation, thereby realizing an adjustable spectral filtering function and multi-band light filtering, thereby effectively replacing several excitation filters required for the original fluorescence channel, and having a light sensor detection function, which can replace the CMOS camera in the imaging part of the original fluorescence channel. One module simultaneously replaces the principle light filtering and receiving functions, and is small in size and low in price.

[0018] 2. It is also very convenient when conducting fluorescence detection. It can cope with PCR reaction plates of different specifications, and perform multi-directional limit fixation on PCR reaction plates of different specifications to achieve rapid clamping and fixation. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] Figure 1 This is a schematic diagram of the principle structure of a fluorescence emitter in a PCR fluorescence signal detector based on MEMS technology of the present invention.

[0021] Figure 2 This is a schematic diagram of the overall structure of a PCR fluorescence signal detector based on MEMS technology in the present invention;

[0022] Figure 3 This is a schematic diagram of the main structure of a PCR fluorescence signal detector based on MEMS technology of the present invention;

[0023] Figure 4 It is a structural schematic diagram of a PCR fluorescence signal detector loading plate pulled out based on MEMS technology of the present invention;

[0024] Figure 5 It is a structural schematic diagram of a PCR fluorescence signal detector loading plate 14 based on MEMS technology of the present invention;

[0025] Figure 6 It is a structural schematic diagram of a quick clamping mechanism 20 of a PCR fluorescence signal detector based on MEMS technology of the present invention;

[0026] Figure 7 It is a structural schematic diagram of another viewing angle of a quick clamping mechanism 20 of a PCR fluorescence signal detector based on MEMS technology of the present invention;

[0027] Figure 8 This is a structural schematic diagram of a hollow semi-cylinder 34 of a PCR fluorescence signal detector based on MEMS technology of the present invention;

[0028] Fig. 9 This is a schematic diagram of the structure of a moving mechanism of a PCR fluorescence signal detector based on MEMS technology in the present invention.

[0029] The symbols in the accompanying drawings are described as follows: body 10; compartment door 11; base pad 12; touch display screen 13; loading plate 14; card plate part 15; slide bar part 16; PCR reaction plate 17; installation slide groove 18; screw 19; quick clamping mechanism 20; clamping plate body 21; opening groove 22; slide column 23; support plate 24; fixed angle plate 25; rubber plate 26; tooth plate 27; bottom platform part 28; cavity 29; vertical plate 30; horizontal tooth plate 31; top plate 32; cylindrical part 33; hollow semi-cylinder 34; trapezoidal protrusion 35; gear cylinder 36; light source mounting plate 41; fluorescent emitter 42; electromagnetic slide rail 43; electromagnetic slide rail driving seat 44; connecting part 45; light source 4201; lower glass plate 4202; silicon base 4203; film 4204; bottom electrode 4205; upper glass plate 4206; top electrode 4207. DETAILED DESCRIPTION

[0030] The present invention will now be further described in detail in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. These drawings are all simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic way, and therefore they only show the structures related to the present invention.

[0031] To facilitate understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present invention are shown in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0032] All features disclosed in this specification, or steps in all methods or processes disclosed, except mutually exclusive features and / or steps, can be combined in any manner.

[0033] Any feature disclosed in this specification (including any additional claims, abstract and drawings), unless otherwise stated, may be replaced by other equivalent or alternative features with similar purposes. That is, unless otherwise stated, each feature is only an example of a series of equivalent or similar features.

[0034] In the present invention, unless otherwise clearly defined and specified, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of at least two elements or the interaction relationship between at least two elements, unless otherwise clearly defined. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] Combine the following Figure 1-9 The present invention is described in detail. For the convenience of description, the directions mentioned below are defined as follows: the up, down, left, right, front, and back directions mentioned below are the same as Figure 3 The view direction is consistent in front, back, left, right, up and down directions. Figure 3 is a front view of the device of the present invention, Figure 3 The directions shown are consistent with the front, back, left, right, up and down directions of the device of the present invention when viewed from the front.

[0036] See also Figure 1-9An embodiment of the present invention is as follows: a PCR fluorescence signal detector based on MEMS technology, comprising a body 10, wherein the bottom is stably supported by four base pads 12, a touch display screen 13 for control is installed on the top, and a loading plate 14 is installed at the bottom of the inner cavity of the body 10, and quick clamping mechanisms 20 for ensuring the stability of fluorescence detection are installed on the left and right sides of the upper end surface of the loading plate 14, respectively, and a PCR reaction plate 17 for fluorescence detection is fixed by using the two quick clamping mechanisms 20 on the left and right sides, so as to facilitate detection, a moving component is installed at the top of the inner cavity of the body 10, and a wavelength-adjustable fluorescence emitter 42 is fixedly installed on the moving component, and the fluorescence emitter 42 includes a silicon base 4203, and a cavity is etched at the bottom of the silicon base 4203, so that a thin film 4204 is formed on the top of the cavity, and at the same time, a thin film 4204 is formed on the upper end surface of the silicon base 4203. A bottom electrode 4205 is deposited, an upper glass plate 4206 is deposited on the upper end surface of the bottom electrode 4205, a top electrode 4207 is deposited on the upper end surface of the upper glass plate 4206, an external power supply is connected between the top electrode 4207 and the bottom electrode 4205, a lower glass plate 4202 is fixedly installed on the bottom of the silicon base 4203, a light source 4201 is arranged on the lower end surface of the lower glass plate 4202, and a drive is generated by adjusting the voltage across the top electrode 4207 and the bottom electrode 4205 to cause the membrane to deform, and the voltage is adjusted to adjust the membrane deformation, thereby realizing an adjustable spectral filtering function, light filtering of multiple bands, such as FAM, VIC, ROX, CY5, etc. If light with a wavelength in the FAM band needs to be emitted, the voltage on the electrode is adjusted to satisfy the passage of light in the FAM band, and if light with a wavelength in the VIC band needs to be emitted, the voltage on the MEMS electrode is adjusted to satisfy the passage of light in the VIC band;

[0037] The top electrode 4207 is downwardly aligned with the bottom PCR reaction plate 17, the light source 4201 is turned on, and the driving power supply is given a suitable voltage (the corresponding band voltage is selected according to demand). At this time, the light of the filtered wavelength enters the reaction hole of the PCR reaction plate 17, and the product to be inspected absorbs the corresponding wavelength and undergoes energy transition. The MEMS detection mode starts to work, and the light intensity and position after the energy transition are detected, so as to judge the performance of the product to be inspected.

[0038] In addition, in one embodiment, the quick clamping mechanism 20 includes a clamping plate body 21, the bottom of the clamping plate body 21 is provided with a bottom platform 28, the upper end surface of the loading plate 14 is provided with a mounting slot 18, the bottom of the bottom platform 28 is provided with protrusions on both sides, the protrusions are respectively embedded in the slots on both sides of the mounting slot 18, and the clamping plate body 21 is provided with an open groove 22, and the semicircular cavities on both sides of the open groove 22 are respectively fixedly connected with sliding columns 23, and the two sliding columns 23 are slidably sleeved with support plates 24, and a return spring is connected between the lower end surface of the support plate 24 and the bottom of the open groove 22, and a fixed angle is rotatably connected to the support plate 24. Plate 25, a cavity 29 is provided in the clamping plate body 21, the cavity 29 is rotatably connected with a gear cylinder 36, a tooth plate 27 is slidably connected to the bottom of the cavity 29, the upper end surface meshing teeth of the tooth plate 27 mesh with the gear cylinder 36, a rubber plate 26 is fixedly connected to the tooth plate 27, a supporting spring is connected between the rubber plate 26 and the outer end surface of the clamping plate body 21, a transverse tooth plate 31 is slidably connected to the upper side wall of the cavity 29, the meshing teeth at the bottom of the transverse tooth plate 31 mesh with the gear cylinder 36, a vertical plate 30 is fixedly connected to the transverse tooth plate 31, the upper end surface of the vertical plate 30 is fixedly connected with a top plate 32, and the bottom of the top plate 32 is slidably connected to the upper end surface of the clamping plate body 21;

[0039] When the PCR reaction plate 17 is placed on the upper end surface of the loading plate 14, the clamping plate bodies 21 on the left and right sides are respectively pressed against the PCR reaction plate 17, so that the rubber plate 26 abuts against the PCR reaction plate 17, and the left and right sides of the PCR reaction plate 17 are limited and clamped, and at the same time, the tooth plate 27 slides into the inner cavity of the cavity 29, and at the same time drives the gear cylinder 36 to rotate, thereby driving the top transverse tooth plate 31 to slide in the direction close to the PCR reaction plate 17, thereby driving the top plate 32 to slide in the direction close to the PCR reaction plate 17, and then the top plate 32 moves against the upper plate of the fixed angle plate 25, so that the fixed angle plate 25 rotates, and the lower pressure plate at the lower end of the fixed angle plate 25 presses the upper end surface of the PCR reaction plate 17, thereby pressing the upper and lower positions of the PCR reaction plate 17, so that the PCR reaction plate 17 is fixed and limited;

[0040] It should be noted that, in this embodiment, in order to further improve the stability of the PCR reaction plate 17, cylindrical portions 33 are fixedly installed on the extensions on both sides of the top plate 32, and support plates 24 are slidably connected to the semicircular cavities on both sides of the opening groove 22, and a compression spring is connected between the lower end surface of each hollow semi-cylinder 34 and the support plate 24, and each hollow semi-cylinder 34 is provided with a trapezoidal protrusion 35, and each cylindrical portion 33 can abut against the corresponding trapezoidal protrusion 35;

[0041] Therefore, when the top plate 32 moves against the upper plate of the fixed angle plate 25, the cylindrical parts 33 on both sides slide and abut against the trapezoidal protrusions 35 respectively, and slide along the inclined surfaces of the trapezoidal protrusions 35, thereby pressing each of the trapezoidal protrusions 35 downward respectively, and finally getting stuck, so that the fixed angle plate 25 can be further pressed at the top to ensure the stability of the PCR reaction plate 17 when it is placed.

[0042] It is worth mentioning that a lead screw 19 is rotatably installed in the installation slot 18, the lead screw 19 passes through the base portion 28, and is threadedly connected to the base portion 28, and a rotating disk is fixedly connected to the left end of the installation slot 18, so as to facilitate the adjustment of the position of the quick clamping mechanism 20.

[0043] In addition, in one embodiment, the moving component includes an electromagnetic slide rail driving seat 44 fixedly mounted on the upper side wall of the inner cavity of the body 10, a connecting portion 45 is installed on the electromagnetic slide rail 43 in the electromagnetic slide rail driving seat 44, and another electromagnetic slide rail driving seat 44 is installed on the connecting portion 45, wherein a light source mounting plate 41 is installed on the electromagnetic slide rail, and a fluorescent emitter 42 is fixedly mounted on the lower end surface of the light source mounting plate 41, and two sets of electromagnetic slide rails are used to control the movement of the fluorescent emitter 42, so that the fluorescent emitter 42 can be accurately moved to irradiate the detection object in the PCR reaction plate 17.

[0044] In addition, in one embodiment, in order to facilitate the placement of the PCR reaction plate 17 on the loading plate 14, the front end surface of the loading plate 14 is provided with a card plate portion 15 on the left and right sides respectively, and the lower end surface of the loading plate 14 is provided with a slide portion 16 at the position of each card plate portion 15, so that people can directly hook the card plate portion 15 with their fingers to pull out the loading plate 14, and a sinking cavity is provided at the bottom of the inner cavity of the body 10, and the extension portion at the bottom of the rear end of the loading plate 14 is slidably connected to the sinking cavity, and the slide portion 16 is slidably connected to the bottom notch at the entrance of the front side end surface of the body 10, so that the loading plate 14 will not be completely pulled out even if force is used.

[0045] When fluorescence detection is required, the compartment door 11 is opened, the loading plate 14 is pulled out, and the PCR reaction plate 17 containing the test sample is first placed on the loading plate 14, and then the lead screw 19 is rotated to make the two quick clamping mechanisms 20 move towards the direction close to the PCR reaction plate 17 respectively, so that the rubber plate 26 abuts against the PCR reaction plate 17, and the left and right sides of the PCR reaction plate 17 are clamped, and at the same time, the tooth plate 27 slides into the inner cavity of the cavity 29, and at the same time drives the gear cylinder 36 to rotate, thereby driving the top transverse tooth plate 31 to slide towards the direction close to the PCR reaction plate 17, thereby driving the top plate 32 to slide towards the direction close to the PCR reaction plate 17, and then the top plate 32 moves against the upper plate of the fixed angle plate 25, so that the fixed angle plate 25 rotates, and the lower pressure plate at the lower end of the fixed angle plate 25 presses the upper end surface of the PCR reaction plate 17, thereby The upper and lower parts of the PCR reaction plate 17 are compressed, so that the PCR reaction plate 17 is fixed and limited. At the same time, when the top plate 32 moves against the upper plate of the fixed angle plate 25, the cylindrical parts 33 on both sides slide against the trapezoidal protrusions 35 respectively, and slide along the inclined surface of the trapezoidal protrusions 35, so that each of the trapezoidal protrusions 35 is pressed down respectively, and finally stuck, so that the fixed angle plate 25 can be further pressed at the top to ensure the stability of the PCR reaction plate 17 when it is placed. The light source 4201 is turned on, and the driving power supply gives a suitable voltage. The corresponding band voltage is selected according to the demand. At this time, the light of the filtered wavelength enters the reaction hole of the PCR reaction plate 17. After the product to be inspected absorbs the corresponding wavelength, energy transition is performed, and the MEMS detection mode starts to work, and the light intensity and position after the energy transition are detected, so as to judge the performance of the product to be inspected, and then the moving component is gradually adjusted to drive the fluorescent emitter 42 to move, and the PCR reaction plate 17 is detected respectively.

[0046] The above is only a specific implementation of the invention, but the protection scope of the invention is not limited to it. Any changes or substitutions that are not conceived through creative work should be included in the protection scope of the invention. Therefore, the protection scope of the invention should be based on the protection scope defined in the claims.

Claims

1. A PCR fluorescence signal detector based on MEMS technology, comprising a body (10), characterized in that: A loading plate (14) is installed at the bottom of the inner cavity of the body (10), and quick clamping mechanisms (20) for ensuring stable fluorescence detection are installed on the left and right sides of the upper end surface of the loading plate (14). A moving component is installed at the top of the inner cavity of the body (10), and a wavelength-adjustable fluorescence emitter (42) is fixedly installed on the moving component. The fluorescence emitter (42) includes a silicon base (4203), and a thin film (4204) is arranged in the silicon base (4203). A bottom electrode (4205) is deposited on the upper end surface of the silicon base (4203), and the upper surface of the bottom electrode (4205) An upper glass plate (4206) is deposited on the end surface, a top electrode (4207) is deposited on the upper end surface of the upper glass plate (4206), an external power supply is connected between the top electrode (4207) and the bottom electrode (4205), a lower glass plate (4202) is fixedly mounted on the bottom of the silicon base (4203), a light source (4201) is arranged on the lower end surface of the lower glass plate (4202), and the deformation of the film (4204) is controlled by adjusting the voltage across the top electrode (4207) and the bottom electrode (4205), thereby adjusting the wavelength of the light emitted by the light source (4201).

2. The PCR fluorescence signal detector based on MEMS technology according to claim 1, characterized in that: The quick clamping mechanism (20) comprises a clamping plate body (21), an open groove (22) is provided on the clamping plate body (21), sliding columns (23) are fixedly connected in the semicircular cavities on both sides of the open groove (22), and a support plate (24) is slidably sleeved on the two sliding columns (23), a return spring is connected between the lower end surface of the support plate (24) and the bottom of the open groove (22), a fixed angle plate (25) is rotatably connected to the support plate (24), a cavity (29) is provided in the clamping plate body (21), a gear cylinder (36) is rotatably connected to the cavity (29), and a tooth plate (27) is slidably connected to the bottom of the cavity (29), and the The meshing teeth on the upper end surface of the tooth plate (27) mesh with the gear cylinder (36); a rubber plate (26) is fixedly connected to the tooth plate (27); a supporting spring is connected between the rubber plate (26) and the outer end surface of the clamp body (21); a transverse tooth plate (31) is slidably connected to the upper side wall of the cavity (29); the meshing teeth at the bottom of the transverse tooth plate (31) mesh with the gear cylinder (36); a vertical plate (30) is fixedly connected to the transverse tooth plate (31); the upper end surface of the vertical plate (30) is fixedly connected to a top plate (32); the bottom of the top plate (32) is slidably connected to the upper end surface of the clamp body (21).

3. The PCR fluorescence signal detector based on MEMS technology according to claim 2, characterized in that: The extensions on both sides of the top plate (32) are respectively fixedly mounted with cylindrical portions (33); the semicircular cavities on both sides of the opening groove (22) are respectively slidably connected with support plates (24); a compression spring is connected between the lower end surface of each hollow semi-cylinder (34) and the support plate (24); each hollow semi-cylinder (34) is respectively provided with a trapezoidal protrusion (35); and each cylindrical portion (33) can abut against the corresponding trapezoidal protrusion (35).

4. The PCR fluorescence signal detector based on MEMS technology according to claim 3, characterized in that: The bottom of the clamping plate body (21) is provided with a base portion (28), the upper end surface of the loading plate (14) is provided with a mounting groove (18), the base portion (28) is slidably connected to the mounting groove (18), a lead screw (19) is rotatably installed in the mounting groove (18), the lead screw (19) passes through the base portion (28) and is threadedly connected to the base portion (28), and a rotating disc is fixedly connected to the left end of the mounting groove (18).

5. The PCR fluorescence signal detector based on MEMS technology according to claim 4, characterized in that: The moving assembly comprises an electromagnetic slide rail driving seat (44) fixedly mounted on the upper side wall of the inner cavity of the body (10), a connecting portion (45) being mounted on the electromagnetic slide rail (43) in the electromagnetic slide rail driving seat (44), another electromagnetic slide rail driving seat (44) being mounted on the connecting portion (45), wherein a light source mounting plate (41) is mounted on the electromagnetic slide rail, and a fluorescent emitter (42) is fixedly mounted on the lower end surface of the light source mounting plate (41).

6. The PCR fluorescence signal detector based on MEMS technology according to claim 5, characterized in that: The front end surface of the loading plate (14) is provided with a clamping plate portion (15) on both sides, and the lower end surface of the loading plate (14) is provided with a sliding strip portion (16) at the position of each clamping plate portion (15).

7. The PCR fluorescence signal detector based on MEMS technology according to claim 6, characterized in that: The inner bottom of the machine body (10) is provided with a sinking cavity, the extension portion of the rear end bottom of the loading plate (14) is slidably connected in the sinking cavity, and the slide portion (16) is slidably connected in the bottom notch at the entrance of the front end surface of the machine body (10).

8. The PCR fluorescence signal detector based on MEMS technology according to claim 7, characterized in that: The bottom of the base portion (28) is provided with protruding portions on both sides, respectively, and the protruding portions are respectively embedded in the slide grooves on both sides of the installation slide groove (18).

9. The PCR fluorescence signal detector based on MEMS technology according to claim 1, characterized in that: A touch display screen (13) is fixedly mounted on the upper end surface of the machine body (10).

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