Plant root system in-situ observation device and observation method

Abstract

The invention discloses a plant root system in-situ observation device and an observation method. The observation device comprises a plant cultivation box, a video camera, a camera and an upper computer, the camera photographs the plant root system of the cultivated plant in the plant cultivation box at a specified period, the camera uploads the photographed picture into the upper computer, and the plant root system analysis software analyzes the image of the plant root system acquired by the camera to obtain the growth trend of the plant root system in the plant cultivation box; the camera shoots the root system of the cultivated plant in the plant cultivation box in a specified plant root system growth trend range of the cultivated plant in the plant cultivation box obtained by the upper computer according to the camera to obtain a dynamic video image of the root system of the plant; and plant root system analysis software in the upper computer analyzes the growth trend of the plant root system. In the scheme, the plant root system image of the cultivated plant is acquired by using the camera to assist the camera, dynamic observation of the plant root system of the cultivated plant is realized, and the observation accuracy of the plant root system is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant root growth observation, and in particular to a plant root in-situ observation device and method. Background Art

[0002] Plant roots have many functions, including absorbing and transporting nutrients and water, synthesizing plant hormones and other organic substances, storing nutrients, and supporting plants to fix them in the soil. It is the main organ for material exchange between plants and the external environment.

[0003] Traditional plant root observation and analysis devices and methods are mostly based on static root images for analysis, with low analysis accuracy. In addition, the existing plant root observation and analysis methods only analyze the growth of plant roots in a transparent culture matrix. However, there is still a large gap between the transparent culture matrix and the actual soil in which the plants grow. This makes the root growth trend obtained by the final analysis quite different from the actual plant growth trend, making it difficult to serve as a guiding basis for large-scale agricultural planting. Summary of the invention

[0004] In order to overcome at least one defect of the above prior art, one of the objects of the present invention is to provide an in-situ observation device for plant roots, which can realize dynamic observation of the plant roots of the cultivated plants by utilizing a camera to assist a camera to obtain images of the plant roots of the cultivated plants, thereby improving the accuracy of plant root observation.

[0005] In order to overcome at least one defect of the prior art, a second object of the present invention is to provide a method for in-situ observation of plant roots, by adding multiple groups of control groups to find the plant root image that is closest to the growth trend of the cultivated plants in the soil.

[0006] The technical solution adopted by the present invention to solve the problem is:

[0007] A plant root in-situ observation device comprises a plant cultivation box, a camera and a camera facing the plant cultivation box, and a host computer connected with the camera and the camera;

[0008] The camera takes pictures of the plant roots of the cultivated plants in the plant cultivation box at a specified period, and the camera uploads the pictures to the host computer, which is installed with plant root analysis software. The plant root analysis software analyzes the images of the plant roots obtained by the camera to obtain the root growth trend of the plant in the plant cultivation box;

[0009] The camera films the roots of the cultivated plants in the plant cultivation box within the specified plant root growth trend range of the cultivated plants in the plant cultivation box obtained by the camera of the host computer, and obtains dynamic video images of the roots of the plants; the plant root analysis software in the host computer analyzes the growth trend of the roots of the plants.

[0010] Furthermore: the plant cultivation box includes a transparent box body and a box cover; the outer surface of the transparent box body is provided with scales; the box cover is provided with a plant fixing groove; the plant fixing groove is provided with a flexible material for fixing the plant into the plant fixing groove.

[0011] Furthermore: the inner diameter of the plant fixing groove is larger than the maximum outer diameter of the stem of the plant corresponding to the cultivated plant, and the cultivated plant is placed in the plant fixing groove after the stem of the cultivated plant is wrapped with a flexible material; the flexible material includes one of a sponge, cotton wool, a paper ball and a foam board.

[0012] Further: the specified period refers to a specified time interval;

[0013] The designated plant root growth trend range means that the plants cultivated in the plant cultivation box are within the rapid growth trend range.

[0014] A method for in-situ observation of plant roots, using the above-mentioned in-situ observation device for plant roots to observe.

[0015] The observation method includes the following steps:

[0016] S1. Prepare several plant cultivation boxes, namely:

[0017] In the first group, the plant cultivation box is filled with a transparent cultivation matrix, and the cultivated plants are directly planted in the transparent cultivation matrix;

[0018] The second group, a substrate permeable sheet is arranged in the plant cultivation box, the substrate permeable sheet divides the plant cultivation box into two independent spaces, one of which is filled with a transparent cultivation substrate, and the other space is planted and cultivated plants;

[0019] The third group, the plant cultivation box is filled with a transparent cultivation matrix, the cultivation plants are directly planted in the transparent cultivation matrix, and the outside of the plant cultivation box is wrapped with a black light shield;

[0020] The fourth group, a substrate permeable sheet is set in the plant cultivation box, the substrate permeable sheet divides the plant cultivation box into two independent spaces, one of which is filled with a transparent cultivation substrate, and the other space is planted and cultivated plants, and a black light shield is wrapped outside the plant cultivation box;

[0021] The fifth group, the plant cultivation box is filled with a transparent cultivation matrix, and microbial strains are added to the transparent cultivation matrix to simulate the soil environment. The cultivated plants are directly planted in the transparent cultivation matrix, and a black light shield is wrapped around the outside of the plant cultivation box;

[0022] The sixth group, the plant cultivation box is filled with an opaque cultivation matrix, and microbial strains are added to the opaque cultivation matrix to simulate the soil environment, and the cultivated plants are directly planted in the opaque cultivation matrix;

[0023] Group 7, the plant cultivation box was filled with soil, and the cultivated plants were directly planted in the soil;

[0024] S2. Place the plant cultivation boxes of the first to fifth groups in the same temperature, humidity, light, and oxygen concentration environment, and install cameras and video cameras on the outside of the plant cultivation boxes of the first to fifth groups;

[0025] S3. Connecting five cameras and five video cameras to a host computer, controlling the working time of the cameras and the video cameras through the host computer, and simultaneously photographing the first to fifth groups of cultivated plants with the five cameras, uploading the plant root images of the cultivated plants to the host computer, and using the plant root analysis software in the host computer to timely analyze the plant root images and output a plant root growth trend graph;

[0026] S4. After the growth cycles of the cultivated plants in the first to seventh groups are all completed, the cultivated plants in the plant cultivation boxes of the sixth and seventh groups are taken out, and the soil on the roots of the plants is taken out, and photos of the roots of the cultivated plants in the sixth and seventh groups are respectively obtained through a camera, and the root photos of the cultivated plants in the sixth and seventh groups are compared with the root photos of the cultivated plants in the first to fifth groups through the plant root analysis software in the host computer, and cultivated plants in the first to fifth groups whose final growth states are similar or close to those of the cultivated plants in the sixth and seventh groups are obtained.

[0027] At the same time, the root growth trends of the cultivated plants from the first to seventh groups are obtained, and the group of cultivated plants with the best root growth trend is obtained.

[0028] Furthermore: the camera records root growth images of the plant roots according to the root growth trend of the corresponding group of cultivated plants obtained by the host computer; when the root growth trend of the corresponding group of plants output by the host computer accelerates, the camera records the root image of the cultivated plant and transmits the recorded dynamic image to the host computer; the plant root analysis software in the host computer analyzes the dynamic image transmitted by the camera to obtain the root growth process of the cultivated plant in this stage.

[0029] Furthermore, when photographing the root systems of the plants in the plant cultivation boxes of the third to fifth groups, the black light shield inside the plant cultivation boxes needs to be temporarily removed, and after the photographing is completed, the black light shield is reset.

[0030] Furthermore: partition plates are provided in the cultivation boxes of the first group and the third group, and the roots of the cultivation plants planted in the transparent cultivation matrix grow on both sides of the partition plates.

[0031] Furthermore: the plant cultivation boxes of the sixth and seventh groups are provided with mesh inner boxes, and the mesh inner boxes are placed inside the cultivation boxes.

[0032] Further: the transparent culture substrate in the first to fifth groups had the same mass, and the amount of the opaque culture substrate in the sixth group and the amount of soil in the seventh group were consistent with the amount of the transparent culture substrate in the first group;

[0033] The size of the planting space for cultivating plants in Groups 1 to 7 is the same.

[0034] In summary, the plant root in-situ observation device provided by the present invention has the following technical effects:

[0035] 1. Use cameras and video cameras to obtain images of the root systems of plants cultivated in the plant cultivation box, that is, use a combination of static and dynamic methods to obtain images of the root systems of the cultivated plants. In particular, the camera shoots the roots of the cultivated plants in the plant cultivation box within the specified plant root growth trend range of the plants cultivated in the plant cultivation box obtained by the camera on the host computer, and obtains dynamic video images of the roots of the plants, ensuring that images of various stages of root growth are obtained, thereby improving the accuracy of the plant root analysis software in the host computer in analyzing the roots of the cultivated plants.

[0036] 2. Use cameras and video cameras to obtain images of the root systems of the plants cultivated in the plant incubator, that is, use a combination of static and dynamic methods to obtain images of the root systems of the cultivated plants. In particular, the camera takes pictures of the roots of the cultivated plants in the plant incubator within the specified plant root growth trend range of the plants cultivated in the plant incubator obtained by the camera on the host computer, thereby avoiding the use of cameras to obtain root images of the cultivated plants throughout the process, thereby reducing the analysis pressure of the plant root analysis software in the host computer.

[0037] In summary, the in-situ observation method of plant roots provided by the present invention has the following technical effects:

[0038] 1. Prepare multiple control groups, cultivate the cultivated plants in multiple control groups respectively, obtain the graphs of the root growth process of the cultivated plants in the multiple control groups, analyze the root growth trend of each plant through plant root analysis software, obtain the growth trend of the cultivated plants, find the cultivated plants in the control group with a growth trend similar or close to that of the plants cultivated in conventional soil, and obtain the root growth trend and plant growth trend of the plants grown in conventional soil.

[0039] 2. Use a camera to record images of the faster growing parts of the plant roots to obtain the growth trend of the plant roots with high analysis accuracy.

[0040] 3. The camera is used to record images of the parts of the cultivated plants with faster root growth trends. The recording time is relatively short compared to the plant growth cycle, so as to minimize the impact on the growth of the cultivated plants in the plant cultivation box wrapped with the black sunshade. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic diagram of the planting of the first group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0042] Figure 2 This is a schematic diagram of the planting of the second group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0043] Figure 3 This is a schematic diagram of the planting of the third group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0044] Figure 4 This is a schematic diagram of the planting of the fourth group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0045] Figure 5 This is a schematic diagram of the planting of the fifth group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0046] Figure 6 This is a schematic diagram of the planting of the sixth group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0047] Figure 7 This is a schematic diagram of the planting of the seventh group of cultivated plants in a method for in-situ observation of plant roots according to the technical solution of the present invention.

[0048] Figure 8 This is a schematic diagram of the box cover structure in a device for in-situ observation of plant roots according to the technical solution of the present invention.

[0049] The meanings of the reference numerals are as follows:

[0050] 1. Plant cultivation box; 2. Transparent cultivation matrix; 3. Divider; 4. Box cover; 5. Matrix penetration sheet; 6. Black light shield; 7. Opaque cultivation matrix; 8. Opaque cultivation matrix with added microbial strains; 9. Mesh inner box; 10. Cultivated plants; 11. Soil; 12. Plant fixing groove; 13. Flexible material. DETAILED DESCRIPTION

[0051] For better understanding and implementation, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0052] In the description of the present invention, it should be noted that the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the present invention.

[0053] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0054] The invention discloses a plant root in-situ observation device and a plant root in-situ observation method. The observation method adopts a plant root in-situ observation device to observe and obtain the root growth trend of the observed plant.

[0055] In this solution, a plant root in-situ observation device includes a plant cultivation box 1, a camera and a camera facing the plant cultivation box 1, and a host computer connected to the camera and the camera.

[0056] The camera takes pictures of the plant roots of the cultivated plants 10 in the plant cultivation box 1 at a specified period, and the camera uploads the pictures to the host computer. The host computer is installed with plant root analysis software, which analyzes the images of the plant roots obtained by the camera to obtain the growth trend of the roots of the plants in the plant cultivation box 1.

[0057] The camera captures the root system of the cultivated plant 10 in the plant cultivation box 1 within the designated plant root growth trend range of the cultivated plant 10 in the plant cultivation box 1 obtained by the camera, and obtains a dynamic video image of the root system of the plant. The plant root analysis software in the host computer analyzes the growth trend of the root system of the plant.

[0058] Specifically, the plant root analysis software in the host computer analyzes the growth trend of the plant's root system based on all images of the plant's root system acquired by the camera and all video images of the plant's root system acquired by the video camera.

[0059] In the above scheme, the root system image of the cultivated plant 10 is obtained by the camera, which is simple and fast. Then the camera transmits the obtained image information to the host computer, and the plant root system analysis software is used for analysis, which is fast.

[0060] In the above scheme, the plant root analysis software uses the real-time image of the plant root system of the cultivated plant 10 obtained by the camera to output the growth trend of the current cultivated plant. If the growth trend of the plant root system of the cultivated plant 10 at this time or in the upcoming time is within the specified plant root growth trend range, the camera is started to dynamically record the image of the plant root system of the cultivated plant 10, obtain the growth image of the plant root system of the cultivated plant 10 at this time or in a certain period of time thereafter, and transmit the image to the host computer. The plant root analysis software in the host computer analyzes the growth trend of the plant root system of the cultivated plant based on the dynamic image of the cultivated plant transmitted by the camera.

[0061] In the above scheme, by using a camera to assist the camera in acquiring images of the plant root systems of the cultivated plants, dynamic observation of the plant root systems of the cultivated plants can be achieved, thereby improving the accuracy of plant root system observation.

[0062] In the above scheme, a camera and a video camera are used to obtain images of the root systems of the plants cultivated in the plant cultivation box, that is, a combination of static and dynamic methods are used to obtain images of the root systems of the cultivated plants. In particular, the camera films the roots of the cultivated plants in the plant cultivation box within the specified plant root growth trend range of the plants cultivated in the plant cultivation box obtained by the camera by the host computer, and obtains dynamic video images of the roots of the plants, ensuring that images of various stages of root growth are obtained, thereby improving the accuracy of the plant root analysis software in the host computer for analyzing the roots of the cultivated plants.

[0063] In the above scheme, a camera and a video camera are used to obtain images of the root systems of the plants cultivated in the plant cultivation box, that is, a combination of static and dynamic methods are used to obtain images of the root systems of the cultivated plants. In particular, the camera films the roots of the cultivated plants in the plant cultivation box within the specified plant root growth trend range of the plants cultivated in the plant cultivation box obtained by the camera by the host computer, thereby avoiding the use of cameras to obtain root images of the cultivated plants throughout the process, thereby reducing the analysis pressure of the plant root analysis software in the host computer.

[0064] In the technical solution, the plant cultivation box 1 includes a transparent box body and a box cover 4. A scale is provided on the outer surface of the transparent box body, which is convenient for a camera or video camera to use as a reference when shooting the plant root system. The scale includes a horizontal scale and a vertical scale, so that the camera or video camera can quickly and accurately obtain the length and expansion amplitude of the plant root system growth, which is convenient for the plant root system analysis software to obtain the root system size and quickly and accurately analyze the growth trend of the plant root system.

[0065] The box cover 4 is provided with a plant fixing groove 12, and a flexible material 13 for fixing the plant to the plant fixing groove 12 is provided in the plant fixing groove 12. The cultivated plants are fixed on the box cover by the flexible material 13 to ensure that the planting depth of the plants is consistent when the plants are cultivated.

[0066] Of course, fertilizer ports, watering ports, drainage ports and exhaust ports should also be provided on the plant cultivation box 1, so that the plant cultivation box 1 is close to the planting environment, and it is convenient to regularly replenish water and fertilizer for the plants cultivated in the plant cultivation box 1 as needed, ensure that the roots of the plants cultivated in the plant cultivation box 1 are ventilated, and ensure the normal growth of the cultivated plants.

[0067] In the present technical solution, the inner diameter of the plant fixing groove 12 is larger than the maximum outer diameter of the stem of the plant corresponding to the cultivated plant 10, so as to avoid the plant fixing groove 12 from restricting and hindering the growth of the cultivated plant. The cultivated plant 10 is placed in the plant fixing groove 12 after the stem of the cultivated plant 10 is wrapped with the flexible material 13. The flexible material 13 includes one of a sponge, cotton wool, a paper ball and a foam board, so as to avoid the plant fixing groove from restricting and hindering the growth of the stem of the plant, and ensure that the cultivated plant can grow freely in all directions in the plant fixing groove 12, and ensure the growth state and environment of the plant.

[0068] In the technical scheme, the designated period refers to a specified time interval. The general camera shooting time interval is 12 hours or 24 hours or other time intervals, or in the early stage of plant growth, the shooting interval is longer, such as 48 hours or 24 hours, or other time intervals, and in the middle of the plant growth cycle, the shooting interval is shorter, such as 12 hours or 6 hours or other time intervals. Taking pictures of cultivated plants in a designated period is not only convenient for accurately obtaining the growth status changes of cultivated plants, but also avoids frequent shooting, which puts a certain pressure on the work of the host computer.

[0069] In the present technical solution, the designated plant root growth trend range refers to the cultivated plant 10 in the plant cultivation box 1 being within the rapid growth trend range. Generally, during the period when the cultivated plant grows faster, the plant root system of the cultivated plant is dynamically photographed by a camera. The plant root analysis software in the host computer analyzes the growth trend of the plant root system based on the plant root system photos of the cultivated plant obtained by the camera. When the plant root system growth trend accelerates, the camera is started to photograph the plant root system to obtain a dynamic image of the plant growth, and then the dynamic image is transmitted to the plant root analysis software in the host computer. The plant root analysis software analyzes the dynamic image to obtain the plant root system growth trend of the cultivated plant.

[0070] A method for in-situ observation of plant roots is provided, wherein the plant root in-situ observation device is used for observation, and the observation method comprises the following steps:

[0071] S1. Prepare several plant cultivation boxes 1, which are:

[0072] In the first group, the plant cultivation box 1 is filled with a transparent cultivation matrix 2 , and the cultivation plants 10 are directly planted in the transparent cultivation matrix 2 .

[0073] In the second group, a substrate permeable sheet 5 is arranged in the plant cultivation box 1, and the substrate permeable sheet 5 divides the plant cultivation box 1 into two independent spaces, one of which is filled with a transparent cultivation substrate 2, and the other space is planted with the cultivated plants 10.

[0074] In the third group, the plant cultivation box 1 is filled with a transparent cultivation matrix 2 , the cultivation plants 10 are directly planted in the transparent cultivation matrix 2 , and the outside of the plant cultivation box 1 is wrapped with a black light shield 6 .

[0075] In the fourth group, a matrix permeable sheet 5 is arranged in the plant cultivation box 1, which divides the plant cultivation box 1 into two independent spaces, one of which is filled with a transparent cultivation matrix 2, and the other space is planted with cultivated plants 10, and a black sunshade 6 is wrapped around the outside of the plant cultivation box 1.

[0076] In the fifth group, the plant cultivation box 1 is filled with a transparent cultivation matrix 2, and microbial strains are added to the transparent cultivation matrix 2 to simulate the soil 11 environment. The cultivation plants 10 are directly planted in the transparent cultivation matrix 2, and a black sunshade 6 is wrapped around the outside of the plant cultivation box 1.

[0077] In the sixth group, the plant cultivation box 1 is filled with an opaque cultivation matrix 8 with added microorganisms to simulate the soil 11 environment, and the cultivation plants 10 are directly planted in the opaque cultivation matrix 8 with added microorganisms.

[0078] In the seventh group, the plant cultivation box 1 is filled with soil 11 , and the cultivation plants 10 are directly planted in the soil 11 .

[0079] S2. Place the plant cultivation boxes 1 of the first to fifth groups in the same temperature, humidity, light, and oxygen concentration environment, and install cameras and video cameras on the outside of the plant cultivation boxes of the first to fifth groups, respectively.

[0080] S3. Connect five cameras and five video cameras to a host computer, and control the working time of the cameras and video cameras through the host computer. The five cameras simultaneously shoot the first to fifth groups of cultivated plants 10 respectively, and upload the plant root images of the cultivated plants 10 to the host computer. The plant root analysis software in the host computer timely analyzes the plant root images and outputs the plant root growth trend chart.

[0081] S4. When the growth cycles of the cultivated plants 10 in the first to seventh groups are all completed, the cultivated plants 10 in the plant cultivation boxes 1 of the sixth and seventh groups are taken out, and the soil 11 on the roots of the plants is taken out, and photos of the plant roots of the cultivated plants 10 in the sixth and seventh groups are respectively obtained through a camera, and the root photos of the cultivated plants 10 in the sixth and seventh groups are compared with the root photos of the cultivated plants 10 in the first to fifth groups through the plant root analysis software in the host computer, so as to obtain the cultivated plants 10 in the first to fifth groups whose final growth states are similar or close to those of the cultivated plants 10 in the sixth and seventh groups respectively.

[0082] At the same time, the root growth trends of the cultivated plants 10 in the first to seventh groups are obtained, and the group of cultivated plants 10 with the best root growth trend is obtained.

[0083] In order to facilitate the observation of the root system of the plant and not affect the normal growth of the plant or damage the root system of the plant during the observation, the present scheme adopts in situ observation of the plant root system, that is, the plant is observed when it is in the planting state. In order to facilitate the observation of the root system of the plant being planted, the present scheme uses a transparent culture matrix to cultivate the plant. The transparent culture matrix does not affect the observation of the root system of the plant inside it by the camera or video camera due to its transparent state, and the image of the plant root system can be obtained. Through the plant root system analysis software, the growth trend of the plant root system can be obtained.

[0084] However, a transparent cultivation matrix is ​​set in the plant cultivation box 1. Even if the ratio of nutrient components in the transparent cultivation matrix is ​​scientific and reasonable, it cannot ensure that it is consistent with the growth environment of the plant in the soil. Therefore, in order to obtain the root growth trend of the plant growing in the soil, the third to seventh test groups are added in this scheme to simulate and obtain the root growth trend of the plant growing in the transparent cultivation matrix, but the growing root system is in a lightless environment, simulate and obtain the root system of the plant growing in the opaque cultivation matrix, simulate and obtain the root system of the plant growing in the opaque cultivation matrix with added microbial strains close to the soil environment, and the root system of the plant growing in the soil.

[0085] That is, in this scheme, multiple control groups are prepared, and cultivated plants are cultivated in multiple control groups respectively, and the graphs of the root growth process of the cultivated plants in the multiple control groups are obtained. The root growth trend of each plant is analyzed by plant root analysis software to obtain the growth trend of the cultivated plants, and the cultivated plants in the control group with growth trends similar or close to those of the plants cultivated in conventional soil are found to obtain the root growth trend and plant growth trend of the plants grown in conventional soil. That is, through such observation methods and operation methods, the growth trend of the root system of the plants planted in the soil can be obtained or basically obtained. That is, the growth trend of the plant root system when the plant is planted in conventional soil is obtained, which has guiding significance for the large-scale planting of the plant.

[0086] In the present technical solution, the camera records the root growth image of the plant root according to the root growth trend of the corresponding group of cultivated plants 10 obtained by the host computer. When the root growth trend of the corresponding group of plants output by the host computer accelerates, the camera records the root image of the cultivated plant 10 and transmits the recorded dynamic image to the host computer. The plant root analysis software in the host computer analyzes the dynamic image transmitted by the camera to obtain the root growth process of the cultivated plant 10 in this stage.

[0087] A general camera dynamically shoots the plants during the period when the root system of the plants grows faster to obtain dynamic images of the root system of the plants. When dynamically shooting the root systems of the plants in the third to fifth groups, it is necessary to open the black light shield 6 outside the plant cultivation box 1 to take pictures, which temporarily changes the environmental parameters for plant growth. To avoid affecting the growth of the plants, it is necessary to immediately reset the black light shield after shooting for a period of time, so that the root system of the plants in the plant cultivation box is in a dark growth environment again, which is similar to the dark environment in the soil.

[0088] Generally, during a period of rapid root growth, a camera is needed to capture two or more growth dynamics of the plant roots so that the plant root analysis software can infer the changing trend of the plant roots during this growth cycle based on the changing trend of root growth, thereby improving the accuracy of the plant root growth trend output by the plant root analysis software.

[0089] For the comparison between the first group and the second group, it is necessary to obtain the growth trend of the plant roots when the plant roots are completely in the transparent culture matrix and when the plant roots are not in the transparent culture matrix and can only absorb the nutrients in the transparent culture matrix for their growth. If the growth trend of the plant roots does not change much between the first group and the second group, the plant can be planted in a transparent culture box filled with soil at a later stage, and the plant can be planted close to an inner surface of the transparent culture box, so that the growth state of part of the plant roots can be obtained through the transparent culture box, and the growth trend of part of the root system of the plant growing in the soil can be obtained.

[0090] In the present technical solution, when photographing the roots of the plants in the plant cultivation boxes 1 of the third to fifth groups, the black light shield 6 inside the plant cultivation boxes 1 needs to be temporarily removed, and the black light shield 6 is reset after the photographing is completed.

[0091] In the technical solution, the first and third groups of cultivation boxes are provided with partitions 3, and the plant roots of the cultivated plants 10 planted in the transparent cultivation matrix 2 grow on both sides of the partition 3. By using the partition, the plant roots of the cultivated plants are separated to prevent the plant roots from being entangled and affecting the shooting effect.

[0092] In the technical scheme, the plant cultivation boxes 1 of the sixth and seventh groups are provided with a mesh inner box 9, which is placed in the cultivation box. The mesh inner box 9 is provided, and after the growth cycle of the cultivated plants is completed, the mesh inner box 9 can be connected with the plants in it and removed from the plant cultivation box 1, and then the soil wrapped on the plant root system or the opaque cultivation matrix 8 with added microbial strains is removed to expose the plant root system, and the integrity of the plant root system is maintained as much as possible to avoid affecting or damaging the plant root system when removing the soil wrapped on the plant root system or the opaque cultivation matrix 8 with added microbial strains.

[0093] In this technical solution, the amount of transparent culture substrate 2 in the first to fifth groups is consistent, and the amount of opaque culture substrate 7 in the sixth group and soil 11 in the seventh group are consistent with the amount of transparent culture substrate 2 in the first group. The size of the planting space for cultivating plants 10 in the first to seventh groups is consistent. The same or substantially consistent growth environment is provided for the plant roots, reducing plant growth variables and improving the accuracy of plant root analysis.

[0094] The technical means disclosed in the scheme of the present invention are not limited to the technical means disclosed in the above-mentioned implementation mode, but also include technical schemes composed of any combination of the above-mentioned technical features. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications are also regarded as the protection scope of the present invention.

Claims

1. A plant root in-situ observation device, characterized in that: It includes a plant cultivation box, a camera and a camera facing the plant cultivation box, and a host computer connected with the camera and the camera; The camera takes pictures of the plant roots of the cultivated plants in the plant cultivation box at a specified period, and the camera uploads the pictures to the host computer, which is installed with plant root analysis software. The plant root analysis software analyzes the images of the plant roots obtained by the camera to obtain the root growth trend of the plant in the plant cultivation box; The camera films the roots of the cultivated plants in the plant cultivation box within the specified plant root growth trend range of the cultivated plants in the plant cultivation box obtained by the camera of the host computer, and obtains dynamic video images of the roots of the plants; the plant root analysis software in the host computer analyzes the growth trend of the roots of the plants.

2. The plant root in-situ observation device according to claim 1, characterized in that: The plant cultivation box comprises a transparent box body and a box cover; the outer surface of the transparent box body is provided with scales; the box cover is provided with a plant fixing groove; and the plant fixing groove is provided with a flexible material for fixing the plant in the plant fixing groove.

3. The plant root in-situ observation device according to claim 2, characterized in that: The inner diameter of the plant fixing groove is larger than the maximum outer diameter of the stem of the plant corresponding to the cultivated plant. The cultivated plant is placed in the plant fixing groove after the stem of the cultivated plant is wrapped with a flexible material; the flexible material includes one of a sponge, cotton wool, a paper ball and a foam board.

4. The plant root in-situ observation device according to claim 1, characterized in that: The specified period refers to a prescribed time interval; The designated plant root growth trend range means that the plants cultivated in the plant cultivation box are within the rapid growth trend range.

5. A method for in-situ observation of plant roots, characterized in that: The plant root in-situ observation device according to any one of claims 1 to 4 is used for observation. The observation method includes the following steps: S1. Prepare several plant cultivation boxes, namely: In the first group, the plant cultivation box is filled with a transparent cultivation matrix, and the cultivated plants are directly planted in the transparent cultivation matrix; The second group, a substrate permeable sheet is arranged in the plant cultivation box, the substrate permeable sheet divides the plant cultivation box into two independent spaces, one of which is filled with a transparent cultivation substrate, and the other space is planted and cultivated plants; The third group, the plant cultivation box is filled with a transparent cultivation matrix, the cultivation plants are directly planted in the transparent cultivation matrix, and the outside of the plant cultivation box is wrapped with a black light shield; The fourth group, a substrate permeable sheet is set in the plant cultivation box, the substrate permeable sheet divides the plant cultivation box into two independent spaces, one of which is filled with a transparent cultivation substrate, and the other space is planted and cultivated plants, and a black light shield is wrapped outside the plant cultivation box; The fifth group, the plant cultivation box is filled with a transparent cultivation matrix, and microbial strains are added to the transparent cultivation matrix to simulate the soil environment. The cultivated plants are directly planted in the transparent cultivation matrix, and a black light shield is wrapped around the outside of the plant cultivation box; The sixth group, the plant cultivation box is filled with an opaque cultivation matrix, and microbial strains are added to the opaque cultivation matrix to simulate the soil environment, and the cultivated plants are directly planted in the opaque cultivation matrix; Group 7, the plant cultivation box was filled with soil, and the cultivated plants were directly planted in the soil; S2. Place the plant cultivation boxes of the first to fifth groups in the same temperature, humidity, light, and oxygen concentration environment, and install cameras and video cameras on the outside of the plant cultivation boxes of the first to fifth groups; S3. Connecting five cameras and five video cameras to a host computer, controlling the working time of the cameras and the video cameras through the host computer, and simultaneously photographing the first to fifth groups of cultivated plants with the five cameras, uploading the plant root images of the cultivated plants to the host computer, and using the plant root analysis software in the host computer to timely analyze the plant root images and output a plant root growth trend graph; S4. After the growth cycles of the cultivated plants in the first to seventh groups are all completed, the cultivated plants in the plant cultivation boxes of the sixth and seventh groups are taken out, and the soil on the roots of the plants is taken out, and photos of the roots of the cultivated plants in the sixth and seventh groups are respectively obtained through a camera, and the root photos of the cultivated plants in the sixth and seventh groups are compared with the root photos of the cultivated plants in the first to fifth groups through the plant root analysis software in the host computer, and cultivated plants in the first to fifth groups whose final growth states are similar or close to those of the cultivated plants in the sixth and seventh groups are obtained. At the same time, the root growth trends of the cultivated plants from the first to seventh groups are obtained, and the group of cultivated plants with the best root growth trend is obtained.

6. The method for in-situ observation of plant roots according to claim 5, characterized in that: The camera records root growth images of the plant roots according to the root growth trends of the corresponding groups of cultivated plants obtained by the host computer. When the root growth trends of the corresponding groups of cultivated plants output by the host computer accelerate, the camera records the root images of the cultivated plants and transmits the recorded dynamic images to the host computer. The plant root analysis software in the host computer analyzes the dynamic images transmitted by the camera to obtain the root growth process of the cultivated plants in this stage.

7. The method for in-situ observation of plant roots according to claim 5, characterized in that: When photographing the root systems of the plants in the plant cultivation boxes of the third to fifth groups, the black light shield inside the plant cultivation boxes needs to be temporarily removed, and the black light shield is reset after the photographing is completed.

8. The method for in-situ observation of plant roots according to claim 5, characterized in that: The cultivation boxes of the first and third groups are provided with partitions, and the roots of the cultivation plants planted in the transparent cultivation substrate grow on both sides of the partitions.

9. The method for in-situ observation of plant roots according to claim 5, characterized in that: The plant cultivation boxes of the sixth and seventh groups are provided with mesh inner boxes, which are placed inside the cultivation boxes.

10. The method for in-situ observation of plant roots according to claim 5, characterized in that: The transparent culture medium in groups 1 to 5 had the same mass, and the amount of opaque culture medium in group 6 and soil in group 7 was consistent with the amount of transparent culture medium in group 1; The size of the planting space for cultivating plants in Groups 1 to 7 is the same.

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