Bacteriostatic itching-relieving lotion and application thereof

Abstract

The invention discloses an antibacterial itching-relieving lotion and application thereof, and belongs to the technical field of biology. The bacteriostatic itching-relieving lotion contains a bacteriostatic itching-relieving composition, and the composition comprises the following components: nanogold, a celery extract and a multi-strain co-culture extract. Wherein the strains used by the multi-strain co-culture extract are lactobacillus gasseri, bifidobacterium and saccharomycetes. Through the synergistic effect of the nanogold, the celery extract and the multi-strain co-culture extract, the antibacterial itching-relieving lotion has a good antibacterial effect and is mild and non-irritating.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an antibacterial and anti-itching lotion and application thereof. Background Art

[0002] Gynecological inflammation is often caused by bacteria or fungi such as Candida albicans, Staphylococcus aureus, Escherichia coli, Neisseria gonorrhoeae, Proteus mirabilis, Trichomonas and Streptococcus agalactiae. These pathogens not only endanger women's health, but also seriously affect their daily lives. However, many traditional female private care products contain strong bactericidal ingredients such as chlorhexidine gluconate, benzalkonium chloride and matrine, which may destroy the normal flora balance of the private parts. Long-term use may cause uncomfortable symptoms such as itching and dryness of the private parts. Therefore, the market is in urgent need of a wash product that can effectively inhibit the growth of pathogens, gently protect the health of the private parts, and does not cause irritation. Summary of the invention

[0003] The purpose of the present invention is to provide an antibacterial and anti-itching lotion and its application. The active substance of the product is compounded with nano-gold, celery extract and multi-strain co-culture extract, which has high safety, strong antibacterial effect, and is mild and non-irritating.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In a first aspect, the present invention provides an antibacterial and anti-itching composition, comprising the following components: nanogold, celery extract, and multi-strain co-culture extract.

[0006] Preferably, the antibacterial and anti-itching composition comprises the following components in parts by weight:

[0007] Nano gold 0.005-0.01 parts;

[0008] 5-10 parts of celery extract;

[0009] 5-10 parts of multi-strain co-culture extract;

[0010] The nano-gold particle size is 20-200 nm;

[0011] The preparation method of the multi-strain co-culture extract comprises the following steps:

[0012] S1. Prepare MRS medium;

[0013] S2. Lactobacillus gasseri, Bifidobacterium and Saccharomyces cerevisiae were added at 2*10 6 CFU / mL was inoculated into a culture container containing MRS liquid medium for co-cultivation under the following culture conditions: temperature 25-35°C, pH 5.0-5.5, culture for 8-12h;

[0014] S3. After the culture is completed, sterile air is introduced into the culture container, and the liquid culture medium in the culture container is supplemented with MRS medium and then the culture is continued for 4-6 hours;

[0015] S4. After the culture is completed, the culture is subjected to ultrasonic disruption, high temperature instant sterilization, filtration, and the filtrate is collected and freeze-dried to obtain a multi-strain co-culture extract;

[0016] in,

[0017] The deposit number of the Lactobacillus gasseri is: CGMCC No.6361;

[0018] The deposit number of the bifidobacterium is: ATCC 15696;

[0019] The yeast has a deposit number of GIMCC 2.85.

[0020] Furthermore, the antibacterial and anti-itching composition is composed of the following components in parts by weight:

[0021] Nano gold 0.005-0.007 parts;

[0022] Celery extract 5-7 parts;

[0023] 5-7 parts of multi-strain co-culture extract;

[0024] The nano-gold particle size is 20-200nm.

[0025] Most preferably, the antibacterial and anti-itching composition is composed of the following components in parts by weight:

[0026] Nanogold 0.007 parts;

[0027] Celery extract 7 parts;

[0028] 7 extracts from multi-strain co-culture;

[0029] The nano-gold particle size is 20-200nm.

[0030] In a second aspect, the present invention provides an antibacterial and anti-itch lotion, comprising the following ingredients: 7-9wt% antibacterial and anti-itch composition, 0.5-5wt% water-soluble vitamin E, 0.1-1.5wt% ascorbyl tetraisopalmitate, 20-25wt% glycerol, and the balance sterile water.

[0031] In a third aspect, the present invention provides a method for preparing an antibacterial and anti-itching lotion, the specific steps of which are as follows:

[0032] S1. dissolving the celery extract, the multi-strain co-culture extract, and the water-soluble vitamin E in sterile water and heating to 40-45° C., and stirring to obtain phase A;

[0033] S2. Add nanogold to sterile water and ultrasonically vibrate until transparent to obtain phase B;

[0034] S3. Mix glycerol and ascorbyl tetraisopalmitate to obtain phase C;

[0035] S4, evenly mix phase A, phase B and phase C, filter, sterilize and fill to obtain an antibacterial and anti-itching lotion.

[0036] Compared with the prior art, the present invention has the following beneficial effects: the present invention mixes nano-gold, celery extract, and multi-species co-culture extract in a specific mass ratio to prepare an antibacterial and anti-itch composition; through the synergistic effect between the components, the antibacterial and anti-itch composition has a good antibacterial effect. Further, the composition is supplemented with glycerol, anti-hepatic acid tetraisopalmitate, water-soluble vitamin E, and sterile water to prepare an antibacterial and anti-itch lotion. The antibacterial and anti-itch lotion can not only effectively inhibit Staphylococcus aureus, Escherichia coli, and Candida albicans, but also has a certain inhibitory effect on Neisseria, Streptococcus agalactiae, Proteus mirabilis, and Trichomonas, and can be used to prevent and treat related diseases caused by the above-mentioned bacterial infections. Experiments show that the antibacterial and anti-itch lotion is mild and non-irritating, and has excellent production and application value. DETAILED DESCRIPTION

[0037] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0038] The test methods without specific conditions in the following tests are usually carried out under conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0039] Some raw materials and their sources are as follows:

[0040] Nanogold (20-200 nm, 500-1000 nm): purchased from Wuhan Kemik Biopharmaceutical Technology Co., Ltd.

[0041] Celery extract: purchased from Xi'an Xinhe Biotechnology Co., Ltd.;

[0042] The preservation number of Lactobacillus gasseri is: CGMCC No.6361.

[0043] The deposit number of Bifidobacterium is: ATCC 15696.

[0044] The yeast deposit number is: GIMCC 2.85.

[0045] MRS medium: purchased from Thermo Fisher Scientific, catalog number: CM1153R.

[0046] Staphylococcus aureus: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number: B81854 (A).

[0047] Escherichia coli: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number B81038.

[0048] Candida albicans: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number BMZ132601.

[0049] Neisseria: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., catalog number BMZ133675.

[0050] Streptococcus agalactiae: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number B80383.

[0051] Proteus mirabilis: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., catalog number BMZ122593.

[0052] Trichomonas: purchased from Xinyang Laiyao Biotechnology Co., Ltd., catalog number ATCC 50143.

[0053] The method for preparing the multi-strain co-culture extract of the present invention is as follows:

[0054] Multi-strain co-culture extract 1:

[0055] S1: Prepare MRS medium according to the instructions for use of MRS medium;

[0056] S2: Lactobacillus gasseri, Bifidobacterium, and Saccharomyces cerevisiae were added at 2*10 6 CFU / mL was inoculated into a 20L fermenter containing 8LMRS liquid medium for co-cultivation under the following conditions: temperature 30°C, pH 5.3, and culture for 10 h;

[0057] S3: Sterile air was introduced into the fermenter after the culture was completed, and 4LMRS culture medium was added and culture was continued for 5 hours; the sterile air flow rate was 1L / min;

[0058] S4: After the cultivation is completed, the culture is subjected to ultrasonic disruption, high-temperature instantaneous sterilization, and filtration. The filtrate is collected and freeze-dried to obtain a multi-species co-culture extract, wherein the ultrasonic disruption conditions are: frequency: 20kHz, time: 20min, power: 650w, temperature: 28°C; high-temperature instantaneous sterilization conditions: temperature 135°C, time 5s; filtration conditions: filtration using a 0.45μm microporous filter membrane.

[0059] Multi-strain co-culture extract 2:

[0060] S1: Prepare MRS medium according to the instructions for use of MRS medium;

[0061] S2: Lactobacillus gasseri, Bifidobacterium, and Saccharomyces cerevisiae were added at 2*10 6 CFU / mL was inoculated into a 20L fermenter containing 8LMRS liquid medium for co-cultivation under the following conditions: temperature 25°C, pH 5.0, and culture for 12 h;

[0062] S3: Sterile air was introduced into the fermenter after the culture was completed, and 4LMRS culture medium was added and culture was continued for 4 hours; the sterile air flow rate was 1L / min;

[0063] S4: After the culture is completed, the culture is subjected to ultrasonic disruption, high-temperature instant sterilization, and filtration. The filtrate is collected and freeze-dried to obtain a multi-strain co-culture extract, wherein the ultrasonic disruption conditions are: frequency: 20kHz, time: 20min, power: 650w, temperature: 28°C; high-temperature instant sterilization conditions: temperature 135°C, time 5s; filtration conditions: filtration using a 0.45μm microporous membrane. Multi-strain co-culture extract 3:

[0064] S1: Prepare MRS medium according to the instructions for use of MRS medium;

[0065] S2: Lactobacillus gasseri, Bifidobacterium, and Saccharomyces cerevisiae were added at 2*10 6 CFU / mL was inoculated into a 20L fermenter containing 8LMRS liquid medium for co-cultivation under the following conditions: temperature 35°C, pH 5.5, and culture for 8h;

[0066] S3: After the culture is completed, sterile air is introduced into the fermenter, and 4LMRS culture medium is added and the culture is continued for 6 hours; the sterile air flow rate is 1 L / min.

[0067] S4: After the cultivation is completed, the culture is subjected to ultrasonic disruption, high-temperature instantaneous sterilization, filtration, and the filtrate is collected and freeze-dried to obtain a multi-species co-culture extract, wherein the ultrasonic disruption conditions are: frequency: 20kHz, time: 20min, power: 650w, temperature: 28°C; high-temperature instantaneous sterilization conditions: temperature 135°C, time 5s; filtration conditions: filtration using a 0.45μm microporous filter membrane.

[0068] Multi-strain co-culture extract①:

[0069] Different from the multi-strain co-culture extract 1, only 3*10 6 CFU / mL Lactobacillus gasseri and 3*10 6CFU / mL yeast (i.e., the total number of viable bacteria inoculated into the culture medium is the same as that in the multi-species co-culture extract ①), and the remaining steps are the same as those in the multi-species co-culture extract 1.

[0070] Multi-strain co-culture extract②:

[0071] Different from the multi-strain co-culture extract 1, only 3*10 6 CFU / mL Lactobacillus gasseri and 3*10 6 CFU / mL Bifidobacterium (i.e., the total number of viable bacteria inoculated into the culture medium is the same as that in the multi-species co-culture extract ①), and the remaining steps are the same as those in the multi-species co-culture extract 1.

[0072] Multi-strain co-culture extract ③:

[0073] Different from the multi-strain co-culture extract 1, only 3*10 6 CFU / mL Bifidobacterium and 3*10 6 CFU / mL yeast (i.e., the total number of viable bacteria inoculated into the culture medium is the same as that in the multi-species co-culture extract ①), and the remaining steps are the same as those in the multi-species co-culture extract 1.

[0074] Multi-strain co-culture extract④:

[0075] Different from the multi-strain co-culture extract 1, only 6*10 6 CFU / mL Bifidobacterium (i.e., the total number of viable bacteria inoculated into the culture medium is the same as that in the multi-species co-culture extract ①), and the remaining steps are the same as those in the multi-species co-culture extract 1.

[0076] Multi-strain co-culture extract⑤:

[0077] Different from the multi-strain co-culture extract 1, only 6*10 6 CFU / mL yeast (i.e., the total number of viable bacteria inoculated into the culture medium is the same as that in the multi-species co-culture extract ①), and the remaining steps are the same as those in the multi-species co-culture extract 1.

[0078] Multi-strain co-culture extract⑥:

[0079] Different from the multi-strain co-culture extract 1, only 6*10 6 CFU / mL Lactobacillus gasseri (i.e., the total number of viable bacteria inoculated into the culture medium is the same as that in the multi-species co-culture extract ①), and the remaining steps are the same as those in the multi-species co-culture extract 1.

[0080] The mass fractions of each component in the antibacterial and anti-itching compositions of the embodiments of the present invention and the comparative examples are shown in Table 1 below:

[0081] Table 1 Mass fraction of each component in the antibacterial and antipruritic composition

[0082]

[0083]

[0084] Note: "-" means no addition, the missing mass parts are replaced by sterile water, the composition The nano-gold particles used in the experiment have a diameter of 500nm-1000nm.

[0085] An antibacterial and itching-reducing lotion, the mass percentages of its components are shown in Table 2 below.

[0086] Table 2 Mass percentage of each component of antibacterial and anti-itch lotion

[0087]

[0088]

[0089]

[0090] The above-mentioned antibacterial and anti-itching lotions 1, 4, 5, 6, 7 and antibacterial and anti-itching lotions The preparation method is as follows:

[0091] S1. dissolving the celery extract, the multi-strain co-culture extract, and the water-soluble vitamin E in sterile water and heating to 43° C., and stirring to obtain phase A;

[0092] S2. Add nanogold to sterile water and ultrasonically vibrate until transparent to obtain phase B;

[0093] S3. Mix glycerol and ascorbyl tetraisopalmitate to obtain phase C;

[0094] S4. Evenly mix phase A, phase B and phase C, filter, sterilize and fill to obtain the antibacterial and itching-removing lotion.

[0095] The preparation method of antibacterial and anti-itching lotion 2 is as follows:

[0096] S1. dissolving the celery extract, the multi-strain co-culture extract, and the water-soluble vitamin E in sterile water and heating to 40° C., and stirring to obtain phase A;

[0097] S2. Add nanogold to sterile water and ultrasonically vibrate until transparent to obtain phase B;

[0098] S3. Mix glycerol and ascorbyl tetraisopalmitate to obtain phase C;

[0099] S4. Evenly mix phase A, phase B and phase C, filter, sterilize and fill to obtain the antibacterial and itching-removing lotion.

[0100] The preparation method of antibacterial and anti-itching lotion 3 is as follows:

[0101] S1. dissolving the celery extract, the multi-strain co-culture extract, and the water-soluble vitamin E in sterile water and heating to 45° C., and stirring to obtain phase A;

[0102] S2. Add nanogold to sterile water and ultrasonically vibrate until transparent to obtain phase B;

[0103] S3. Mix glycerol and ascorbyl tetraisopalmitate to obtain phase C;

[0104] S4. Evenly mix phase A, phase B and phase C, filter, sterilize and fill to obtain the antibacterial and itching-removing lotion.

[0105] Preparation method of blank control of antibacterial and itchy lotion:

[0106] First, water-soluble vitamin E is dissolved in sterile water and heated to 43°C, and stirred evenly to obtain phase A; then glycerol and ascorbyl tetraisopalmitate are mixed evenly to obtain phase B; finally, phase A and phase B are mixed evenly, filtered, sterilized, and filled to obtain antibacterial and anti-itching lotion.

[0107] Antibacterial and anti-itch wash

[0108] Antibacterial and anti-itch wash The formula used is exactly the same as that of Antibacterial and Anti-itch Lotion 1. The difference from Antibacterial and Anti-itch Lotion 1 is that its preparation method is as follows:

[0109] Celery extract, multi-strain co-culture extract, water-soluble vitamin E, nanogold, glycerin, ascorbyl tetraisopalmitate and sterile water are mixed evenly, heated to 43° C., filtered, sterilized and filled to obtain an antibacterial and itching-removing lotion.

[0110] Example 1: Antibacterial test

[0111] Test samples: Antibacterial and Anti-itch Lotion 1-7, Antibacterial and Anti-itch Lotion The mixture was blank with antibacterial and itchy lotion.

[0112] The present invention refers to the microbial killing experimental method of "Disinfection Technical Specification" (2002 edition), and uses test samples to measure the killing rates of Candida albicans, Escherichia coli, Staphylococcus aureus, Neisseria, Streptococcus agalactiae, Proteus mirabilis, and Trichomonas. The results are shown in Table 1 below.

[0113] Table 1 Antibacterial test results

[0114]

[0115]

[0116] From the results in Table 1, it can be seen that antibacterial and itching lotions 1-7 have good inhibitory effects on Escherichia coli, Staphylococcus aureus, and Candida albicans, and also have certain inhibitory effects on Neisseria gonorrhoeae, Streptococcus agalactiae, Proteus mirabilis, and Trichomonas. Comparison of the antibacterial effects of antibacterial and itching lotion 1 with antibacterial and itching lotions ①-③ and ④-⑥ shows that there is a certain synergistic effect between different bacterial species in the multi-species co-culture extract; comparison of antibacterial and itching lotion 1 with antibacterial and itching lotion 4-⑥ shows that there is a certain synergistic effect between different bacterial species in the multi-species co-culture extract; The results showed that there was a certain synergistic effect between nanogold, celery extract and multi-strain co-culture extract; by comparing antibacterial and itching lotion 1 with antibacterial and itching lotion The results show that the particle size of nano-gold has a certain influence on the antibacterial and itchy washing liquid; comparing the antibacterial and itchy washing liquid 1 with the antibacterial and itchy washing liquid The results show that the preparation method provided by the present invention can further improve the antibacterial effect of the antibacterial and itchy lotion.

[0117] Example 2: Vaginal mucosal irritation test

[0118] Tested substances: Antibacterial and Anti-itch Lotion 1-7, Antibacterial and Anti-itch Lotion And the antibacterial and itchy wash blank.

[0119] Experimental method: Refer to the vaginal mucosal irritation experimental method in the "Disinfection Technical Specifications" (2002 edition) to test the irritation of the test substances to the vaginal mucosa.

[0120] Table 2 Grading standards for adverse skin reactions

[0121]

[0122]

[0123] Based on the above test results, the antibacterial and itching-killing lotions 1-7 and antibacterial and itching-killing lotions prepared by the present invention The blank of antibacterial and anti-itch wash had no irritation to the vaginal mucosa.

[0124] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An antibacterial and anti-itching composition, characterized in that: The antibacterial and anti-itching composition is composed of the following components in parts by weight: Nano gold 0.005-0.01 parts; 5-10 parts of celery extract; 5-10 parts of multi-strain co-culture extract; The nano gold is nano gold powder with a particle size of 20-200 nm; The preparation method of the multi-strain co-culture extract comprises the following steps: S1. Prepare MRS medium; S2. Lactobacillus gasseri, Bifidobacterium and Saccharomyces cerevisiae were added at 2*10 6 CFU / mL was inoculated into a culture container containing MRS liquid medium for co-cultivation under the following culture conditions: temperature 25-35°C, pH 5.0-5.5, and culture for 8-12h; S3. After the culture is completed, sterile air is introduced into the culture container, and the liquid culture medium in the culture container is supplemented with MRS medium and then the culture is continued for 4-6 hours; S4. After the culture is completed, the culture is subjected to ultrasonic disruption, high temperature instant sterilization, filtration, and the filtrate is collected and freeze-dried to obtain a multi-strain co-culture extract; in, The deposit number of the Lactobacillus gasseri is: CGMCC No.6361; The deposit number of the bifidobacterium is: ATCC 15696; The yeast has a deposit number of GIMCC 2.

85.

2. The antibacterial and anti-itching composition according to claim 1, characterized in that: The antibacterial and anti-itching composition is composed of the following components in parts by weight: Nano gold 0.005-0.007 parts; Celery extract 5-7 parts; 5-7 portions of multi-strain co-culture extracts.

3. The antibacterial and anti-itching composition according to claim 1, characterized in that: The antibacterial and anti-itching composition is composed of the following components in parts by weight: 0.007 parts of nano-gold; Celery extract 7 parts; 7 extracts from multi-strain co-culture.

4. An antibacterial and anti-itching lotion, characterized in that: The antibacterial and anti-itching lotion comprises 7-9 wt % of the antibacterial and anti-itching composition according to any one of claims 1-3.

5. The antibacterial and anti-itching lotion according to claim 4, characterized in that: Also includes the following components: Water-soluble vitamin E 0.5-5wt%; Ascorbyl tetraisopalmitate 0.1-1.5wt%; Glycerol 20-25wt%; Sterile water balance.

6. A method for preparing an antibacterial and anti-itching lotion, characterized in that: The following steps are involved: S1. Dissolve the celery extract, the multi-strain co-culture extract, and the water-soluble vitamin E in sterile water and heat to 40-45° C., stir evenly, and obtain phase A; S3. Add nanogold to sterile water and ultrasonically vibrate until transparent to obtain phase B; S3. Mix glycerol and ascorbyl tetraisopalmitate to obtain phase C; S4. Mix phase A, phase B and phase C evenly, filter, sterilize and fill.

Images