Bird's nest acid composition and application thereof in cosmetics

Abstract

The invention relates to a sialic acid composition and application thereof in cosmetics. The sialic acid composition comprises carnosine, sialic acid and hydroxyethyl piperazine ethanesulfonic acid. The sialic acid composition is applied to cosmetics, the mass percent of carnosine, sialic acid and hydroxyethyl piperazine ethanesulfonic acid in the cosmetics is (0.1%-10%): (0.1%-2%): (0.1%-4%), and the pH value of the cosmetics is 6-7. Through reasonable matching of the sialic acid, the stability of the sialic acid can be improved, the problems of inactivation and discoloration of the sialic acid are solved, the pH value is stable, viscosity reduction is avoided, meanwhile, synergistic interaction is achieved, and the good whitening effect is achieved when the sialic acid is applied to the human body.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and in particular relates to a bird's nest acid composition and application thereof in cosmetics. Background Art

[0002] "Skin as white as cream" and "skin as white as snow" have always been considered beautiful by Orientals since ancient times. However, most of the commonly used whitening and anti-freckle raw materials on the market have stability problems such as discoloration and inactivation under high temperature and light conditions, or have defects such as low safety, poor permeability, and unclear effects. Finding new whitening raw materials and developing whitening cosmetics that are stable, non-discoloring, safe, and effective are issues that need to be solved urgently.

[0003] Bird's nest acid, also known as sialic acid, is an acylated derivative of carboxylated monosaccharides containing 9 carbon atoms. It is usually located in the key position of the outermost carbohydrate part of the cell membrane and secreted glycoconjugates (glycolipids, glycoproteins and lipopolysaccharides), and is an important material basis for the diversification of glycoconjugate structures and functions. Bird's nest acid is naturally present in animals, plants and microorganisms, and can be obtained by extraction, or prepared by chemical synthesis, enzymatic synthesis and microbial fermentation.

[0004] Bird's nest acid has been proven to have many biological functions, such as promoting the brain development of infants and young children, fighting neurodegenerative diseases, fighting viruses and bacterial infections, etc. It has been widely used in food, health care, medicine and other fields. In recent years, studies have shown that the active hydroxyl group of bird's nest acid can combine with peroxides, and has good antioxidant, anti-inflammatory and anti-aging effects, and has attracted more and more attention from researchers in the cosmetics field. Bird's nest acid not only has excellent antioxidant capacity, but also has the effect of inhibiting tyrosinase activity and melanosome maturation, and inhibits the production of melanin by promoting melanin degradation through selective autophagy (Yoshikawa K, Maeda KN-AcetylneuraminicAcidInhibitsMelanogenesis viaInduction ofAutophagy[J].Cosmetics,2024,11(3):82-.), indicating that bird's nest acid should also have potential whitening effects.

[0005] However, during the experiment, it was found that bird's nest acid is easily inactivated and accompanied by discoloration under high temperature and light conditions, which greatly affects the efficacy and appearance of the cosmetics used.

[0006] Therefore, how to improve the stability of bird's nest acid under high temperature and light conditions and give full play to its efficacy is a technical problem that needs to be solved urgently. Summary of the invention

[0007] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a stable bird's nest acid composition and its application in cosmetics, specifically a stable, non-discoloring bird's nest acid composition and its application in whitening cosmetics. By compounding carnosine and hydroxyethylpiperazine ethane sulfonic acid, the stability of bird's nest acid is significantly improved, the inactivation and discoloration problems of bird's nest acid are improved, and the pH value is stable and the viscosity is not reduced. At the same time, the three synergistically enhance the effect, and it has a good whitening effect when applied to the human body.

[0008] The present invention is achieved through the following technical solutions:

[0009] The first object of the present invention is to provide a bird's nest acid composition, which comprises carnosine, bird's nest acid and hydroxyethylpiperazine ethane sulfonic acid. The composition has good stability, is not easily inactivated or discolored under high temperature and light conditions, has a stable pH value, does not reduce viscosity, and has a synergistic whitening effect.

[0010] In one embodiment of the present invention, the pH value of the bird's nest acid composition is 6-7.

[0011] The second object of the present invention is to provide a method for preparing the swallow acid composition, comprising the following steps:

[0012] Carnosine and bird's nest acid are sequentially added to deionized water, stirred and dissolved until clear and transparent, and then hydroxyethylpiperazineethane sulfonic acid is added, and stirring is continued until clear and transparent to obtain the bird's nest acid composition.

[0013] The third object of the present invention is to provide the use of the bird's nest acid composition in cosmetics.

[0014] In one embodiment of the present invention, the cosmetics are whitening cosmetics.

[0015] In one embodiment of the present invention, the cosmetic is one or more of an aqueous solution, an essence, a facial mask, a cream, an emulsion and a foundation.

[0016] In one embodiment of the present invention, the mass percentages of carnosine, saccharic acid and hydroxyethylpiperazineethane sulfonic acid in the cosmetic are 0.1% to 10%: 0.1% to 2%: 0.1% to 4%.

[0017] In one embodiment of the present invention, the mass percentages of carnosine, saccharic acid and hydroxyethylpiperazineethane sulfonic acid in the cosmetic are 0.1% to 6%: 0.1% to 2%: 0.1% to 4%.

[0018] In one embodiment of the present invention, the temperature at which the bird's nest acid composition is added into the cosmetic is ≤45°C.

[0019] In one embodiment of the present invention, the pH value of the cosmetic is 6-7.

[0020] In one embodiment of the present invention, the cosmetic further comprises a water-soluble antioxidant and a chelating agent to enhance the stability of high-dosage amount of bird's nest acid.

[0021] In one embodiment of the present invention, the content of the water-soluble antioxidant is 0.02% to 0.10%; the content of the chelating agent is 0.02% to 0.10%.

[0022] In one embodiment of the present invention, the water-soluble antioxidant is selected from one or more of sodium bisulfite, sodium pyrosulfite, sodium sulfite and sodium thiosulfate.

[0023] In one embodiment of the present invention, the chelating agent is selected from one or more of disodium EDTA, tetrasodium EDTA, tetrasodium glutamate diacetate, capryl hydroxybenzoic acid, sodium phytate and sodium glucoheptonate.

[0024] Carnosine is a dipeptide widely found in the skeletal muscle and brain of vertebrates. It is composed of β-alanyl-L-histidine and is a strong and effective free radical scavenger. It can prevent oxidative damage, inhibit glycosylation, promote collagen synthesis, delay telomere shortening, inhibit mitochondrial damage, and has anti-jaundice and anti-aging effects. The imidazole ring in the carnosine molecule has the ability to chelate metal ions and interrupt free radical reactions. At the same time, carnosine is also an effective biological buffer that reduces the activity of free radicals generated by iron ions by increasing the pH value.

[0025] Hydroxyethylpiperazineethane sulfonic acid is a very effective biological buffer with strong buffering capacity. It can stabilize the pH value of the system for a long time. It also has the function of gently softening keratin (promoting the metabolism of skin keratinocytes) and promoting the penetration and absorption of other active ingredients, thereby improving bioavailability.

[0026] During the experiment, it was found that bird's nest acid is also very easy to inactivate and discolor under high temperature and light conditions, which greatly affects the appearance and efficacy of the cosmetics used. Comparative experiments show that adding chelating agents, water-soluble antioxidants and increasing the pH value can improve the inactivation and discoloration problems of bird's nest acid to varying degrees. Since carnosine combines chelating agents, antioxidants (water-soluble), and biological buffers, it is an ideal additive to improve the inactivation and discoloration problems of bird's nest acid. Comparative experiments show that by adding a certain amount of carnosine, the inactivation and discoloration problems of bird's nest acid can be significantly improved, and by combining it with hydroxyethylpiperazine ethane sulfonic acid, which has the effect of promoting the penetration and absorption of the two, the whitening effect can be significantly improved.

[0027] The above technical solution of the present invention has the following advantages compared with the prior art:

[0028] The present invention provides a stable bird's nest acid composition and its application in cosmetics. The present invention applies the bird's nest acid composition to cosmetics, wherein the mass percentages of carnosine, bird's nest acid and hydroxyethylpiperazineethane sulfonic acid in the cosmetics are 0.1% to 10%: 0.1% to 2%: 0.1% to 4%, and the pH value of the cosmetics is 6 to 7. The stability of bird's nest acid can be improved, the inactivation and discoloration problems of bird's nest acid can be improved, and the pH value is stable, the viscosity is not reduced, and synergistic synergy is achieved. When applied to the human body, it has a good whitening effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to make the content of the present invention more clearly understood, the present invention is further described in detail below according to specific embodiments of the present invention in conjunction with the accompanying drawings, wherein:

[0030] Figure 1 This is the appearance of the samples of Example 2 and Comparative Example 1 in the test examples of the present invention observed under light and 50°C conditions for 28 days;

[0031] Figure 2 This is a graph showing changes in the content of bird's nest acid in the samples of Example 2 and Comparative Example 1 before and after testing at 50° C. in the test examples of the present invention;

[0032] Figure 3 This is a graph showing the change in ITA° values ​​before and after use of samples of Example 4, Comparative Example 7 and Comparative Example 8 in the test examples of the present invention;

[0033] Figure 4 This is a graph showing changes in MI values ​​before and after use of samples of Example 4, Comparative Example 7 and Comparative Example 8 in the test examples of the present invention. DETAILED DESCRIPTION

[0034] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0035] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods, and the materials, reagents, etc. used are all available from commercial sources unless otherwise specified.

[0036] Examples 1-5

[0037] The formulations of Examples 1-5 are shown in Table 1:

[0038] Table 1 Example formula table

[0039]

[0040] The sample preparation methods of each embodiment are as follows:

[0041] 1) Accurately weigh each component material;

[0042] 2) Pretreatment: add carnosine and bird's nest acid to an appropriate amount of water in sequence, stir and dissolve until clear and transparent, then add hydroxyethylpiperazineethanesulfonic acid, continue stirring until clear and transparent to obtain a bird's nest acid composition, and set aside;

[0043] 3) Mix the materials of phase A and heat to 85°C, then keep warm and stir evenly;

[0044] 4) Mix the phase B materials and add them to the phase A materials obtained in step 3), start homogenization, homogenize at high speed for 5 minutes, then cool down and stir;

[0045] 5) When the temperature drops to 45° C., add the bird's nest acid composition obtained in step 2) and the remaining materials of phase C in sequence;

[0046] 6) When the temperature drops to 37°C, take samples for testing and discharge the materials after they pass the test.

[0047] Comparative Examples 1-8

[0048] The formulations of Comparative Examples 1-8 are shown in Table 2.

[0049] Table 2 Comparative Example Formula

[0050]

[0051] The preparation methods of the comparative samples are as follows:

[0052] 1) Accurately weigh each component material;

[0053] 2) Pretreatment: If carnosine, saccharin, hydroxyethylpiperazine ethane sulfonic acid, sodium hydroxide and sodium pyrosulfite are contained in the formula, they are dissolved in a proper amount of water to obtain a saccharin-containing aqueous solution for later use;

[0054] 3) Mix the materials of phase A and heat to 85°C, then keep warm and stir evenly;

[0055] 4) Mix the phase B materials and add them to the phase A materials obtained in step 3), start homogenization, homogenize at high speed for 5 minutes, then cool down and stir;

[0056] 5) When the temperature drops to 45° C., add the aqueous solution containing bird's nest acid obtained in step 2) and the remaining materials in phase C in sequence;

[0057] 6) When the temperature drops to 37°C, take samples for testing and discharge the materials after they pass the test.

[0058] Test Example 1: Test of bird's nest acid content

[0059] Cosmetics containing bird's nest acid are very unstable under high temperature and light conditions. Especially under high temperature conditions, they will be inactivated and accompanied by discoloration. Over time, the color will gradually change from the initial colorless to slightly yellow, light yellow, oatmeal, light reddish brown or light brown, reddish brown or brown, etc. After 4 weeks of stability inspection, the appearance changes from dark to light in the order of 50℃>45℃>37℃>light>cycle (-18℃ / 50℃)>room temperature

[0060] >4℃=-18℃, which will affect the efficacy and appearance of the applied cosmetics to a certain extent. Figure 1 As shown:

[0061] pass Figure 1 It can be seen that the sample of Comparative Example 1, which has no protective measures and contains only bird's nest acid, changes color more deeply under high temperature and light conditions. After 28 days at 50°C, the appearance is light brown, and after 28 days under light conditions, the appearance is light reddish brown, which greatly affects the beauty of the cosmetics. The sample of Example 2 containing the bird's nest acid composition of the present invention has almost no change in appearance under high temperature and light conditions.

[0062] Using a high performance liquid chromatograph at a wavelength of 210 nm, the absorption peaks of saccharin in the samples of Example 2 and Comparative Example 1 were detected at the initial stage and at 50°C for 7 days, 14 days, and 28 days. The content of saccharin was calculated by the standard curve method. The results are as follows: Figure 2 shown.

[0063] Figure 2 The results show that when bird's nest acid is added directly to the formula without any protective measures (Comparative Example 1 sample), it will gradually become inactivated under high temperature conditions, and its content will decrease by 13.58% in 28 days. However, by compounding carnosine and hydroxyethylpiperazineethane sulfonic acid in Example 2 sample, its content only decreases by 1.03% under the same high temperature conditions. Figure 1 It can be seen that the inactivation of bird's nest acid (change in content) can lead to color changes. The color change can be used to more intuitively evaluate the stability of bird's nest acid in cosmetic formulas.

[0064] By comparing the bird's nest acid content of the samples of Example 2 and Comparative Example 1 under high temperature conditions, it is shown that the bird's nest acid composition of the present invention can effectively protect the stability of bird's nest acid and reduce the inactivation of bird's nest acid under high temperature, light and other conditions.

[0065] Test Example 2: Stability Test

[0066] The stability tests of the samples of the embodiments and comparative examples were carried out under the conditions of 50°C, light, cycle (-18 / 50°C), 45°C, 37°C, room temperature, 4°C and -18°C, among which 45°C, 37°C, 4°C and room temperature were tested for 12 weeks, and the other conditions were tested for 4 weeks. Some of the results are shown in Table 3.

[0067] Table 3 Stability inspection record

[0068]

[0069]

[0070] Note: OK means no color change.

[0071] From the appearance of each embodiment and comparative example sample in Table 3, it can be seen that the samples of embodiments 1-5 have very good high temperature and light stability, the high temperature and light stability of the samples of comparative examples 4, 6 and 7 are poor, and the samples of comparative examples 1-3, 5 and 8 have very poor high temperature and light stability.

[0072] It can be seen from the comparative examples 1-3 that the chelating agents disodium EDTA and tetrasodium glutamate diacetate can slightly improve the stability of bird's nest acid, but there is a certain viscosity reduction problem due to the ionicity.

[0073] It can be seen from the comparative examples 1, 4 and 5 that the oil-soluble antioxidant tocopherol (vitamin E) cannot improve the stability of bird's nest acid, and the water-soluble antioxidant sodium metabisulfite can improve the stability of bird's nest acid, but it also has the problem of viscosity reduction due to its ionicity and produces an unpleasant odor.

[0074] It can be seen from the samples of Comparative Examples 1 and 6 that the pH regulator sodium hydroxide can improve the stability of bird's nest acid, but it also has a viscosity reduction problem due to its ionicity, and the pH value is unstable. Since Comparative Example 7 does not have hydroxyethylpiperazineethane sulfonic acid to stabilize the pH value, the pH value increases from 6.97 to 7.14, and the appearance also changes from white to light yellow.

[0075] By comparing the pH values ​​before and after the tests of all the examples and comparative examples, it can be seen that the pH values ​​of the samples of Examples 1-5 and Comparative Example 8 containing hydroxyethylpiperazineethane sulfonic acid increased slightly, but the amplitude was small, all less than 0.1; while the pH values ​​of the samples of Comparative Examples 1-7 without hydroxyethylpiperazineethane sulfonic acid decreased in the vast majority, and the amplitude was large, all greater than 0.1, especially for Comparative Examples 3 and 5-7, the pH value change amplitude was greater than 0.15. By comparing the pH value changes of the samples before and after the above investigation, it can be shown that the pH value of bird's nest acid can be stabilized by adding hydroxyethylpiperazineethane sulfonic acid, avoiding the large changes in the pH value of bird's nest acid, which may cause changes in the appearance and viscosity of the product or bring potential irritation.

[0076] Test Example 3: Security Test

[0077] With reference to the human skin patch test in the "Technical Specification for Safety of Cosmetics" (2015), human patch tests were conducted on samples of Example 2, Comparative Example 7 and Comparative Example 8. Only 2 samples of Comparative Example 8 showed weak erythema within 30 minutes after the patch tester was removed, while none of the 30 people tested for samples of Example 4 and Comparative Example 7 showed adverse skin reactions, indicating that the bird's nest acid composition of the present invention has good safety.

[0078] Test Example 4 Whitening Efficacy Test

[0079] The test was carried out in accordance with the specific requirements of the first method of cosmetic freckle removal and whitening efficacy test (UV-induced human skin melanization model freckle removal and whitening efficacy test method) in the "Technical Specifications for Safety of Cosmetics". 30 volunteers who met the requirements were selected, and the skin of the appropriate area on their backs was divided into 2.5cm*2.5cm test areas with an interval of 1.50cm. A solar simulator (SOLARLIGHT 601) was used to irradiate the area at 0.75cm*0.75cm in the center of these areas once a day at a dose of 0.75 times the MED for 4 consecutive days. After 4 days of melanization, the areas with an ITA° value less than 5 different from the entire test area were selected and randomly divided into 5 smear or blank areas: Example 4, Comparative Example 7, Comparative Example 8, positive control (containing 7% ascorbic acid standard sample) and negative control (blank control). For 4 consecutive weeks, 12.5±0.3mg of the corresponding sample (the coating amount was 2.00±0.05mg / cm2) was applied to each 2.5cm*2.5cm area every morning and evening. 2 ). In a constant temperature (20±1℃) and humidity (50±10%) environment, the skin ITA° value and melanin MI value of the test site were tested using a skin colorimeter (Colorimeter CL400) and a skin melanin detector (MexameterMX18) before (0 week) and 1 week, 2 weeks, 3 weeks, and 4 weeks after applying the samples. The measured data were statistically analyzed using PASS software.

[0080] (1) ITA° values ​​and differences of volunteers before and after using samples

[0081] Table 4 ITA° values ​​of samples before and after use

[0082]

[0083]

[0084] From Table 4 and Figure 3It can be seen that compared with before using the sample of Example 4, the ITA° values ​​increased by 2.70%, 18.42%, 31.08% and 42.04% after using the sample of Example 4 for 1 week, 2 weeks, 3 weeks and 4 weeks, respectively, and there was a significant improvement after using it for 2 weeks, 3 weeks and 4 weeks compared with the negative control (P < 0.05);

[0085] Compared with the sample before using comparative example 7, the ITA° values ​​increased by 0.20%, 8.65%, 25.15% and 35.28% after using comparative example 7 sample for 1 week, 2 weeks, 3 weeks and 4 weeks respectively, and there was a significant improvement after using it for 3 weeks and 4 weeks compared with the negative control (P < 0.05);

[0086] Compared with before using the sample of comparative example 8, the ITA° values ​​increased by 0.10%, 6.23%, 19.78% and 29.18% after using the sample of comparative example 8 for 1 week, 2 weeks, 3 weeks and 4 weeks respectively, among which there was a significant improvement compared with the negative control after 3 weeks and 4 weeks of use (P < 0.05).

[0087] In summary, it can be seen that compared with the negative control, the ITA° values ​​of Example 4, Comparative Examples 7 and 8 are all improved to a certain extent, especially Example 4, which is significantly better than Comparative Examples 7 and 8; relative to Comparative Example 7, the use of the sample of Example 4 after 1 week, 2 weeks, 3 weeks, and 4 weeks increased by 1250%, 112.95%, 23.58% and 19.16%, respectively, indicating that the combined use of carnosine, bird's nest acid and hydroxyethylpiperazine ethane sulfonic acid can significantly improve the ITA° value of volunteers, and has a good synergistic whitening effect.

[0088] (2) MI values ​​and differences of volunteers before and after using samples

[0089] Table 5 MI values ​​of samples before and after use

[0090]

[0091] From Table 5 and Figure 4 It can be seen that compared with before using the sample of Example 4, the MI values ​​decreased by 0.60%, 5.72%, 15.28% and 22.81% after using the sample of Example 4 for 1 week, 2 weeks, 3 weeks and 4 weeks, respectively, and there was a significant improvement after using it for 3 weeks and 4 weeks compared with the negative control (P < 0.05);

[0092] Compared with the sample before using Comparative Example 7, the MI values ​​decreased by 0.46%, 4.72%, 13.68% and 19.52% after using the sample of Comparative Example 7 for 1 week, 2 weeks, 3 weeks and 4 weeks, respectively, and there was a significant improvement after using it for 3 weeks and 4 weeks compared with the negative control (P < 0.05);

[0093] Compared with before using the sample of comparative example 8, the MI values ​​decreased by 0.10%, 3.85%, 11.61% and 17.65% after using the sample of comparative example 8 for 1 week, 2 weeks, 3 weeks and 4 weeks respectively, among which there was a significant improvement compared with the negative control after 4 weeks of use (P < 0.05).

[0094] In summary, it can be seen that compared with the negative control, the MI values ​​of Example 4, Comparative Examples 7 and 8 all decreased to a certain extent, especially Example 4, which was significantly better than Comparative Examples 7 and 8; relative to Comparative Example 7, the MI values ​​of the sample of Example 4 decreased by 30.43%, 21.19%, 11.70% and 16.85% after 1 week, 2 weeks, 3 weeks and 4 weeks, respectively, indicating that the combined use of carnosine, bird's nest acid and hydroxyethylpiperazine ethane sulfonic acid can significantly reduce the MI value of volunteers, and has a good synergistic whitening effect.

[0095] (3) Regression coefficient analysis

[0096] The regression coefficient analysis showed that the skin melanin of Example 4, Comparative Example 7 and Comparative Example 8 was significantly improved compared with the negative control (P < 0.05), indicating that the samples of Example 4, Comparative Example 7 and Comparative Example 8 all had certain whitening effects, but the overall whitening effect was Example 4 > Comparative Example 7 > Comparative Example 8, indicating that the combination of carnosine, bird's nest acid and hydroxyethylpiperazine ethane sulfonic acid had a certain synergistic whitening effect.

[0097] In summary, the bird's nest acid composition provided by the present invention has good high temperature and light stability.

[0098] (stable pH value, no discoloration, no viscosity reduction), good safety, and good synergistic whitening effect. It can solve the instability problem of bird's nest acid and the current whitening cosmetics discoloration and unsatisfactory efficacy problems, and is highly practical.

[0099] Obviously, the above embodiments are merely examples for clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the protection scope of the invention.

Claims

1. A bird's nest acid composition, characterized in that: The bird's nest acid composition comprises carnosine, bird's nest acid and hydroxyethylpiperazineethane sulfonic acid.

2. The bird's nest acid composition according to claim 1, characterized in that The pH value of the bird's nest acid composition is 6-7.

3. The method for preparing the swallow nest acid composition according to claim 1 or 2, characterized in that: The following steps are involved: Carnosine and bird's nest acid are sequentially added to deionized water, stirred and dissolved until clear and transparent, and then hydroxyethylpiperazineethane sulfonic acid is added, and stirring is continued until clear and transparent to obtain the bird's nest acid composition.

4. Use of the bird's nest acid composition according to claim 1 or 2 in cosmetics.

5. The use according to claim 4, characterized in that: The cosmetics are one or more of water solution, essence, facial mask, cream, lotion and foundation.

6. The use according to claim 4, characterized in that: The mass percentages of carnosine, saccharic acid and hydroxyethylpiperazineethane sulfonic acid in the cosmetics are 0.1% to 10%: 0.1% to 2%: 0.1% to 4%.

7. The use according to claim 4, characterized in that: The temperature at which the bird's nest acid composition is added into cosmetics is ≤45°C.

8. The use according to claim 4, characterized in that: The pH value of the cosmetic is 6-7.

9. The use according to claim 4, characterized in that: The cosmetic also includes a water-soluble antioxidant and a chelating agent.

10. The use according to claim 9, characterized in that: The water-soluble antioxidant is selected from one or more of sodium bisulfite, sodium pyrosulfite, sodium sulfite and sodium thiosulfate; And / or, the chelating agent is selected from one or more of disodium EDTA, tetrasodium EDTA, tetrasodium glutamate diacetate, caprylhydroxybutyric acid, sodium phytate and sodium glucoheptonate.

Images