Method for treating mariculture wastewater

Abstract

The invention discloses a method for treating mariculture wastewater. Comprising the following steps: S1, carrying out standing treatment on the mariculture wastewater, and collecting supernate to obtain to-be-treated liquid; s2, respectively loading the first compound bacteria liquid and the second compound bacteria liquid on the trimethylchlorosilane modified porous active carrier to obtain a first biological carrier and a second biological carrier; s3, the first biological carrier is put into the to-be-treated liquid to react for 20-30 h, air is introduced for aeration during reaction, and a pretreatment liquid is obtained through filtering; s4, putting a second biological carrier into the pretreatment liquid to react for 12-24 hours, introducing ozone for aeration during the reaction, and filtering to obtain purified seawater; the first compound bacteria liquid is mainly formed by mixing a chain-forming halobacterium liquid and a rhodobacter sacchariphagocytophilus liquid; and the second composite bacterial liquid is mainly formed by mixing a marine Harthrosphaeroides bacterial liquid, a halophilic brine spherical bacterium bacterial liquid and an ocean monomonas bacterial liquid. According to the invention, a physical method and a biological method are combined, so that the purification effect on wastewater is greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of wastewater treatment, and in particular to a method for treating seawater aquaculture wastewater. Background Art

[0002] In recent years, with the improvement of people's living standards, the demand for fresh seafood has increased, resulting in a shortage of offshore fishery resources. This has prompted the rapid development of my country's seafood aquaculture industry. As a major fishery aquaculture country, artificial aquaculture production accounts for an important proportion of the total seafood production in the Chinese market. With the intensive development requirements of the aquaculture industry, the safety and quality of aquatic products, aquaculture water pollution, and water recycling have also received more and more attention.

[0003] Compared with conventional sewage such as freshwater aquaculture wastewater, domestic sewage, and industrial wastewater, the typical point of marine aquaculture wastewater is that the salt content is high, the salty wastewater is usually difficult to treat, and the types and structures of pollutants in the water are complex. In addition, the water contains a large amount of organic matter and nutrients from fish excrement and feed residues. Taking the survival safety and quality of seafood as the starting point, the main problem of marine aquaculture wastewater is to remove these nutrients. The main factors of aquaculture seawater pollution include bait residues, chemical pollution, biological secretions and excrement, etc., which cause an increase in nutrient elements in the water body, an increase in nitrogen and phosphorus content, and BOD and ammonia nitrogen indicators, which exceed the environmental carrying capacity and cause eutrophication of the water body, affecting the normal life of aquaculture organisms.

[0004] Conventional treatment methods generally include physical methods, chemical methods and biological methods. The physical method is the most basic treatment method and is easy to operate. It can usually be divided into filtration, sedimentation, flotation, foam separation, etc. The chemical method for treating marine aquaculture wastewater mainly includes redox technology and coagulation technology. Except for ventilation and electrification, the other methods require the introduction of chemical additives to remove harmful pollutants and bacteria in the water through principles such as adsorption or sedimentation. The biological method can utilize the ability of aquatic plants to directly absorb nitrogen and phosphorus in aquaculture seawater through photosynthesis using developed root systems or stems and leaves, and to enrich microorganisms and hang them on a membrane to form a biofilm to treat wastewater. Using a single treatment method cannot achieve a good treatment effect. Therefore, the present application provides a method for treating marine aquaculture wastewater, which combines physical and biological methods to treat marine aquaculture wastewater. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the present invention proposes a method for treating marine aquaculture wastewater to solve the problems raised by the above-mentioned background technology.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A method for treating marine aquaculture wastewater comprises the following steps:

[0008] S1, allowing the marine aquaculture wastewater to stand for treatment, collecting the supernatant to obtain a liquid to be treated;

[0009] S2, respectively loading the first composite bacterial solution and the second composite bacterial solution on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier and a second biological carrier;

[0010] S3, putting the first biological carrier into the liquid to be treated to react for 20 to 30 hours, during which time air is introduced for aeration, and filtering to obtain a pre-treated liquid;

[0011] S4, placing the second biological carrier into the pre-treatment liquid for reaction for 12 to 24 hours, during which ozone aeration is introduced, and filtering to obtain purified seawater;

[0012] The first composite bacterial solution is mainly composed of 1.5×10 5 ~2.0×10 8 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 4 ~1.8×10 6 The bacterial liquid of R. saccharophagous salts with CFU / mL was mixed in a mass ratio of (0.5-2):(3-5);

[0013] Preferably, Salinimicrobiumcatena (Latin: Salinimicrobiumcatena) is purchased from Shanghai Collection Biotechnology Center; Halorubrum saccharovorum (Latin: Halorubrum saccharovorum) is purchased from Shanghai Xuanke Biotechnology Co., Ltd.

[0014] The second composite bacterial solution is mainly composed of 3.0×10 2 ~3.5×10 4 CFU / mL of marine spiral bacteria solution, with a concentration of 1.0×10 3 ~1.2×10 6 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 1.0×10 6 ~2.5×10 9 The CFU / mL Oceanomonas bacterial solution is mixed in a mass ratio of (0.3-1):(0.3-0.8):(4-6).

[0015] Preferably, Thalassospiramarina (Latin: Thalassospiramarina) was purchased from Shanghai Collection Biotechnology Center, product specification: SHBCC D82786; Salinisphaera halophila (Latin: Salinisphaera halophila) was purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number: BMZ133794; Oceanimonas (Latin: Oceanimonas.sp) was purchased from Shanghai Collection Biotechnology Center, product specification: SHMCC D18298.

[0016] Preferably, the porous active carrier in step S2 is one of mesoporous silicon, mesoporous carbon or ceramsite.

[0017] Preferably, the preparation method of the trimethylchlorosilane-modified porous active carrier in step S2 includes calcining the porous active carrier at 600-700°C for 6-12h, cooling to 60-80°C and then adding it to 5-8mol / L acetic acid for soaking for 5-10h, and rinsing it with deionized water after soaking until the filtrate is neutral to obtain a pretreated carrier; placing the pretreated carrier in trimethylchlorosilane, shaking for 10-30min, standing for 12-20h and then filtering, and drying the obtained carrier to obtain a trimethylchlorosilane-modified porous active carrier.

[0018] Preferably, the mass ratio of the pretreated support to trimethylchlorosilane is (5-8):(9-13).

[0019] Preferably, the loading in step S2 is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution and the second composite bacterial solution respectively, adsorb by shaking for 10 to 15 hours, and then stand for 20 to 25 hours to obtain the first biological carrier and the second biological carrier.

[0020] Preferably, the trimethylchlorosilane-modified porous active carrier is infiltrated with the first composite bacterial solution and the second composite bacterial solution at a mass ratio of 1:(3-6).

[0021] Preferably, in step S3, the weight ratio of the first biological carrier to the liquid to be treated is 1:(200-500).

[0022] Preferably, in step S3, the air aeration time is 3 to 8 hours, the pH of the liquid to be treated is adjusted to 7.0 to 7.5, and the dissolved oxygen of the liquid to be treated is maintained at 1 to 6 mg / L.

[0023] Preferably, in step S4, the weight ratio of the second biological carrier to the pretreatment liquid is 1:(1000-1500).

[0024] Preferably, the amount of ozone added in step S4 is 15-25% of the total volume of the pre-treated liquid, and the ozone concentration is 25-50 mg / L.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The treated liquid is subjected to two different degrees of biological adsorption using biological carriers. First, the first biological carrier reacts with the liquid to be treated and is aerated with air, during which a large amount of oxygen is provided to increase the dissolved oxygen content in the water for consumption by microorganisms when degrading organic matter. At the same time, the bottom wastewater is transmitted upward through bubbles to accelerate the reaction between the microorganisms in the first composite bacterial liquid and the wastewater, effectively reducing the content of nutrients such as nitrogen and phosphorus in the wastewater. Secondly, the pre-treated liquid is treated with the second composite bacterial liquid and ozone, and the cooperation of the second composite bacterial liquid and ozone is used to further remove the content of ammonia nitrogen, nitrite and organic matter in the water. The technical solution of this application has a great impact on suspended matter, COD Mn 、BOD 5 The removal rates of inorganic nitrogen and active phosphate were 89.74-91.98%, 98.45-98.65%, 97.83-98.0%, 98.45-99.02% and 99.90-99.97%, respectively. DETAILED DESCRIPTION

[0027] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0028] Example 1

[0029] A method for treating marine aquaculture wastewater comprises the following steps:

[0030] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0031] S2. The first composite bacterial solution and the second composite bacterial solution are respectively loaded on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier and a second biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 600°C for 6 hours, cooling to 60°C and then adding it to 5mol / L acetic acid for immersion for 5 hours, and rinsing with deionized water until the filtrate is neutral after the immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a ratio of 5:9, shaken for 10 minutes, filtered after standing for 12 hours, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution and the second composite bacterial solution at a ratio of 1:3, shake and adsorb for 10 hours, and then stand for 20 hours to obtain the first biological carrier and the second biological carrier;

[0032] S3, putting the first bio-carrier into the liquid to be treated to react for 20 hours, during which air is introduced for aeration for 3 hours, adjusting the pH of the liquid to be treated to 7.0, maintaining the dissolved oxygen of the liquid to be treated at 1 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first bio-carrier to the liquid to be treated is 1:200;

[0033] S4, adding the second biological carrier into the pretreatment liquid to react for 12 hours, during which 25 mg / L of ozone is introduced for aeration, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pretreatment liquid is 1:1000; and the amount of ozone added is 15% of the total volume of the pretreatment liquid;

[0034] The first composite bacterial solution is mainly composed of 1.5×10 5 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 4 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed at a mass ratio of 0.5:3;

[0035] The second composite bacterial solution is mainly composed of 3.0×10 2 CFU / mL of marine spiral bacteria solution, with a concentration of 1.0×10 3 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 1.0×10 6 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 0.3:0.3:4.

[0036] The porous active carrier is mesoporous silica.

[0037] Example 2

[0038] A method for treating marine aquaculture wastewater comprises the following steps:

[0039] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0040] S2. The first composite bacterial solution and the second composite bacterial solution are respectively loaded on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier and a second biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 700°C for 12 hours, cooling to 80°C and then adding it to 8mol / L acetic acid for immersion for 10 hours, and rinsing with deionized water until the filtrate is neutral after the immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a ratio of 8:13, shaken for 30 minutes, filtered after standing for 20 hours, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution and the second composite bacterial solution at a ratio of 1:6, shake and adsorb for 15 hours, and then stand for 25 hours to obtain the first biological carrier and the second biological carrier;

[0041] S3, putting the first biological carrier into the liquid to be treated to react for 30 hours, during which air is introduced for aeration for 8 hours, adjusting the pH of the liquid to be treated to 7.5, maintaining the dissolved oxygen of the liquid to be treated at 6 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first biological carrier to the liquid to be treated is 1:500;

[0042] S4, adding the second biological carrier into the pretreatment liquid to react for 24 hours, during which 50 mg / L of ozone is aerated, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pretreatment liquid is 1:1500; and the amount of ozone added is 25% of the total volume of the pretreatment liquid;

[0043] The first composite bacterial solution is mainly composed of 2.0×10 8 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.8×10 6 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed at a mass ratio of 2:5;

[0044] The second composite bacterial solution is mainly composed of 3.5×10 4 CFU / mL of marine spiral bacteria solution, with a concentration of 1.2×10 6 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.5×10 9 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 1:0.8:6.

[0045] The porous active carrier is mesoporous carbon.

[0046] Example 3

[0047] A method for treating marine aquaculture wastewater comprises the following steps:

[0048] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0049] S2. The first composite bacterial solution and the second composite bacterial solution are respectively loaded on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier and a second biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 650°C for 9h, cooling it to 70°C and then adding it to 7mol / L acetic acid for immersion for 8h, and rinsing it with deionized water until the filtrate is neutral after the immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a mass ratio of 7:10, shaken for 20min, filtered after standing for 18h, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution and the second composite bacterial solution at a mass ratio of 1:5, shake and adsorb for 12h, and then stand for 22h to obtain the first biological carrier and the second biological carrier;

[0050] S3, putting the first bio-carrier into the liquid to be treated to react for 25 hours, during which air is introduced for aeration for 5 hours, adjusting the pH of the liquid to be treated to 7.1, maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first bio-carrier to the liquid to be treated is 1:500;

[0051] S4, adding the second biological carrier into the pretreatment liquid to react for 20 hours, during which 40 mg / L of ozone is introduced for aeration, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pretreatment liquid is 1:1200; and the amount of ozone added is 20% of the total volume of the pretreatment liquid;

[0052] The first composite bacterial solution is mainly composed of 1.8×10 7 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 5 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed at a mass ratio of 1:4;

[0053] The second composite bacterial solution is mainly composed of 3.2×10 3 CFU / mL of marine spiral bacteria solution, with a concentration of 1.1×10 5 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.0×10 8 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 0.5:0.7:5.

[0054] The porous active carrier is ceramsite.

[0055] Comparative Example 1

[0056] The difference between this comparative example and Example 3 is that the porous active carrier is not modified, and the other components and steps remain unchanged.

[0057] A method for treating marine aquaculture wastewater comprises the following steps:

[0058] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0059] S2, respectively loading the first composite bacterial solution and the second composite bacterial solution on ceramsite to obtain the first biological carrier and the second biological carrier; wherein the loading is to soak the ceramsite in the first composite bacterial solution and the second composite bacterial solution at a mass ratio of 1:5, respectively, and adsorb by shaking for 12 hours, and then stand for 22 hours to obtain the first biological carrier and the second biological carrier;

[0060] S3, putting the first bio-carrier into the liquid to be treated to react for 25 hours, during which air is introduced for aeration for 5 hours, adjusting the pH of the liquid to be treated to 7.1, maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first bio-carrier to the liquid to be treated is 1:500;

[0061] S4, adding the second biological carrier into the pretreatment liquid to react for 20 hours, during which 40 mg / L of ozone is introduced for aeration, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pretreatment liquid is 1:1200; and the amount of ozone added is 20% of the total volume of the pretreatment liquid;

[0062] The first composite bacterial solution is mainly composed of 1.8×10 7 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 5 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed at a mass ratio of 1:4;

[0063] The second composite bacterial solution is mainly composed of 3.2×10 3 CFU / mL of marine spiral bacteria solution, with a concentration of 1.1×10 5 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.0×10 8 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 0.5:0.7:5.

[0064] Comparative Example 2

[0065] The difference between this comparative example and Example 3 is that the first biological carrier is not added in step S3, and the other components and steps remain unchanged.

[0066] A method for treating marine aquaculture wastewater comprises the following steps:

[0067] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0068] S3, passing air into the liquid to be treated for aeration for 5 hours, adjusting the pH of the liquid to be treated to 7.1, maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L, and filtering to obtain the pre-treated liquid;

[0069] S2, load the composite bacterial liquid on a trimethylchlorosilane-modified porous active carrier to obtain a biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 650°C for 9h, cooling it to 70°C and then adding it to 7mol / L acetic acid for immersion for 8h, and rinsing it with deionized water until the filtrate is neutral after the immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a mass ratio of 7:10, shaken for 20min, filtered after standing for 18h, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the composite bacterial liquid at a mass ratio of 1:5, shake and adsorb for 12h, and then stand for 22h to obtain a biological carrier;

[0070] S4, adding the bio-carrier into the pre-treatment liquid to react for 20 hours, during which 40 mg / L of ozone is introduced for aeration, and filtering to obtain purified seawater; wherein the weight ratio of the bio-carrier to the pre-treatment liquid is 1:1200; and the amount of ozone added is 20% of the total volume of the pre-treatment liquid;

[0071] The composite bacterial solution is mainly composed of 3.2×10 3 CFU / mL of marine spiral bacteria solution, with a concentration of 1.1×10 5 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.0×10 8 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 0.5:0.7:5.

[0072] The porous active carrier is ceramsite.

[0073] Comparative Example 3

[0074] The difference between this comparative example and Example 3 is that the first biological carrier is used in both steps S3 and S4, and the other components and steps remain unchanged.

[0075] A method for treating marine aquaculture wastewater comprises the following steps:

[0076] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0077] S2. Load the first composite bacterial solution on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 650°C for 9h, cooling it to 70°C and then adding it to 7mol / L acetic acid for immersion for 8h, and rinsing it with deionized water until the filtrate is neutral after immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a mass ratio of 7:10, shaken for 20min, filtered after standing for 18h, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution at a mass ratio of 1:5, shake and adsorb for 12h, and then stand for 22h to obtain the first biological carrier;

[0078] S3, putting the first bio-carrier into the liquid to be treated to react for 25 hours, during which air is introduced for aeration for 5 hours, adjusting the pH of the liquid to be treated to 7.1, maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first bio-carrier to the liquid to be treated is 1:500;

[0079] S4, putting the first biological carrier into the pretreatment liquid to react for 20 hours, during which 40 mg / L of ozone is aerated, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pretreatment liquid is 1:1200; the amount of ozone added is 20% of the total volume of the pretreatment liquid;

[0080] The first composite bacterial solution is mainly composed of 1.8×10 7 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 5 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed at a mass ratio of 1:4;

[0081] The porous active carrier is ceramsite.

[0082] Comparative Example 4

[0083] The difference between this comparative example and Example 3 is that the second biological carrier is used in both steps S3 and S4, and the other components and steps remain unchanged.

[0084] A method for treating marine aquaculture wastewater comprises the following steps:

[0085] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0086] S2. Load the second composite bacterial solution on a trimethylchlorosilane-modified porous active carrier to obtain a second biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 650°C for 9h, cooling it to 70°C and then adding it to 7mol / L acetic acid for immersion for 8h, and rinsing it with deionized water until the filtrate is neutral after immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a mass ratio of 7:10, shaken for 20min, filtered after standing for 18h, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the second composite bacterial solution at a mass ratio of 1:5, shake and adsorb for 12h, and then stand for 22h to obtain a second biological carrier;

[0087] S3, putting the second biological carrier into the liquid to be treated to react for 25 hours, during which air is introduced for aeration for 5 hours, adjusting the pH of the liquid to be treated to 7.1, maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first biological carrier to the liquid to be treated is 1:500;

[0088] S4, adding the second biological carrier into the pretreatment liquid to react for 20 hours, during which 40 mg / L of ozone is introduced for aeration, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pretreatment liquid is 1:1200; and the amount of ozone added is 20% of the total volume of the pretreatment liquid;

[0089] The second composite bacterial solution is mainly composed of 3.2×10 3 CFU / mL of marine spiral bacteria solution, with a concentration of 1.1×10 5 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.0×10 8 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 0.5:0.7:5.

[0090] The porous active carrier is ceramsite.

[0091] Comparative Example 5

[0092] The difference between this comparative example and Example 3 is that air aeration is used in step S4 instead of ozone aeration, and the other components and steps remain unchanged.

[0093] A method for treating marine aquaculture wastewater comprises the following steps:

[0094] S1. Allow the marine aquaculture wastewater to stand for 48 hours and collect the supernatant to obtain a liquid to be treated;

[0095] S2. The first composite bacterial solution and the second composite bacterial solution are respectively loaded on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier and a second biological carrier; wherein the preparation method of the trimethylchlorosilane-modified porous active carrier comprises roasting the porous active carrier at 650°C for 9h, cooling it to 70°C and then adding it to 7mol / L acetic acid for immersion for 8h, and rinsing it with deionized water until the filtrate is neutral after the immersion to obtain a pretreated carrier; the pretreated carrier is placed in trimethylchlorosilane, mixed at a mass ratio of 7:10, shaken for 20min, filtered after standing for 18h, and the obtained carrier is dried to obtain a trimethylchlorosilane-modified porous active carrier. The loading is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution and the second composite bacterial solution at a mass ratio of 1:5, shake and adsorb for 12h, and then stand for 22h to obtain the first biological carrier and the second biological carrier;

[0096] S3, putting the first bio-carrier into the liquid to be treated to react for 25 hours, during which air is introduced for aeration for 5 hours, adjusting the pH of the liquid to be treated to 7.1, maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L, and filtering to obtain a pre-treated liquid; wherein the weight ratio of the first bio-carrier to the liquid to be treated is 1:500;

[0097] S4, putting the second biological carrier into the pre-treated liquid to react for 20 hours, during which air is introduced for aeration for 5 hours, and filtering to obtain purified seawater; wherein the weight ratio of the second biological carrier to the pre-treated liquid is 1:1200; adjusting the pH of the liquid to be treated to 7.1, and maintaining the dissolved oxygen of the liquid to be treated at 3 mg / L;

[0098] The first composite bacterial solution is mainly composed of 1.8×10 7 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 5 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed at a mass ratio of 1:4;

[0099] The second composite bacterial solution is mainly composed of 3.2×10 3 CFU / mL of marine spiral bacteria solution, with a concentration of 1.1×10 5 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.0×10 8 The CFU / mL Oceanomonas bacterial solution was mixed in a mass ratio of 0.5:0.7:5.

[0100] The porous active carrier is ceramsite.

[0101] Comparative Example 6

[0102] The difference between this comparative example and Example 3 is that the bacterial liquid ratio in the first composite bacterial liquid is 1:1 by mass, and the other components and steps remain unchanged.

[0103] The first composite bacterial solution is mainly composed of 1.8×10 7 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 5 CFU / mL of saccharohalorhizobacter bacterial liquid was mixed in a mass ratio of 1:1;

[0104] Comparative Example 7

[0105] The difference between this comparative example and Example 3 is that the bacterial liquid ratio in the second composite bacterial liquid is 1:1:1 by mass, and the other components and steps remain unchanged.

[0106] The second composite bacterial solution is mainly composed of 3.2×10 3 CFU / mL of marine spiral bacteria solution, with a concentration of 1.1×10 5 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 2.0×10 8 The bacterial liquids of Oceanomonas with CFU / mL were mixed in a mass ratio of 1:1:1.

[0107] Comparative Example 8

[0108] The difference between this comparative example and Example 3 is that the bacterial species in the second composite bacterial liquid are changed to Pseudomonas aeruginosa and Marinobacterium dehydrogenase, and the other components and steps remain unchanged.

[0109] The second composite bacterial solution is mainly composed of 2.0×10 9 CFU / mL of marine Pseudomonas bacterial solution and a concentration of 2.0×10 9 The hydrocarbon-removing Marinobacterium liquid with a CFU / mL was mixed in a mass ratio of 1:1.

[0110] Experimental Example 1

[0111] The wastewater from shrimp aquaculture was tested and discharged according to the method and first-level standard of SC / T9103-2007 "Discharge Requirements for Marine Aquaculture Water". The suspended matter in the wastewater before treatment was 312mg / L, pH 9.5, and chemical oxygen demand COD Mn 400mg / L, biochemical oxygen demand BOD 5 120 mg / L, zinc 12.6 mg / L, copper 15.4 mg / L, inorganic nitrogen 22.6 mg / L, active phosphate 31.2 mg / L, sulfide 12.3 mg / L and total residual chlorine 18.7 mg / L, and then treated using the methods of Examples 1 to 3 of the present invention and Comparative Examples 1 to 8, respectively, and the contents of various indicators in the treated wastewater are detected, and the removal rate = (before detection - after detection) / before detection

[0112] The test results are shown in Table 1:

[0113] Table 1 Comparison of different treatment methods for aquaculture wastewater

[0114]

[0115]

[0116] As can be seen from Table 1, the methods of Examples 1 to 3 of the present invention have a good treatment effect on aquaculture wastewater, and the index content of the aquaculture wastewater obtained can meet the first-level standard of "Discharge Requirements for Marine Aquaculture Water", and can be discharged into the first and second class sea areas specified in GB3097. The suspended matter and COD of Example 1 can be obtained by calculation. Mn 、BOD 5 The removal rates of inorganic nitrogen and active phosphate were 89.74%, 98.45%, 97.83%, 98.45% and 99.96% respectively; the removal rates of suspended matter, COD Mn 、BOD 5 The removal rates of inorganic nitrogen and active phosphate were 90.38%, 98.5%, 98.0%, 98.62% and 99.90% respectively; the removal rates of suspended matter, COD Mn 、BOD 5 , inorganic nitrogen and active phosphate removal rates are 91.98%, 98.65%, 97.91%, 99.02% and 99.97% respectively, and the treatment effect on wastewater is obvious. Comparison between Example 3 and Comparative Example 1 shows that the porous active carrier modified by trimethylchlorosilane can not only adsorb more microorganisms, but also increase the high efficiency selectivity of the carrier; Comparison between Example 3 and Comparative Example 2 shows that the first biological carrier has a greater impact on wastewater treatment, and can pre-adsorb wastewater to reduce the pressure of subsequent treatment; Comparison between Example 3 and Comparative Examples 3 and 4 shows that the technical solution is selective in the order of use of the first biological carrier and the second biological carrier; Comparison between Example 3 and Comparative Example 5 shows that the second biological carrier and ozone treatment have a synergistic effect; Comparison between Example 3 and Comparative Examples 6 and 7 shows that the technical solution has a certain selectivity for the proportion of bacterial agent addition, and only by selecting a suitable proportion can the expected treatment effect be achieved; Comparison between Example 3 and Comparative Example 8 shows that the effect of the technical solution can be achieved by not selecting any bacterial species.

[0117] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for treating marine aquaculture wastewater, characterized in that: The following steps are involved: S1, allowing the marine aquaculture wastewater to stand for treatment, collecting the supernatant to obtain a liquid to be treated; S2, respectively loading the first composite bacterial solution and the second composite bacterial solution on a trimethylchlorosilane-modified porous active carrier to obtain a first biological carrier and a second biological carrier; S3, putting the first biological carrier into the liquid to be treated to react for 20 to 30 hours, during which time air is introduced for aeration, and filtering to obtain a pre-treated liquid; S4, placing the second biological carrier into the pre-treatment liquid for reaction for 12 to 24 hours, during which ozone aeration is introduced, and filtering to obtain purified seawater; The first composite bacterial solution is mainly composed of 1.5×10 5 ~2.0×10 8 CFU / mL of chain-forming Halobacterium solani and a concentration of 1.0×10 4 ~1.8×10 6 The bacterial liquid of R. saccharophagous salts with CFU / mL was mixed in a mass ratio of (0.5-2):(3-5); The second composite bacterial solution is mainly composed of 3.0×10 2 ~3.5×10 4 CFU / mL of marine spiral bacteria solution, with a concentration of 1.0×10 3 ~1.2×10 6 CFU / mL of halophilic saltwater spherical bacteria solution and a concentration of 1.0×10 6 ~2.5×10 9 The CFU / mL Oceanomonas bacterial solution is mixed in a mass ratio of (0.3-1):(0.3-0.8):(4-6).

2. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: In step S2, the porous active carrier is one of mesoporous silicon, mesoporous carbon or ceramsite.

3. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: The preparation method of the trimethylchlorosilane-modified porous active carrier in step S2 includes calcining the porous active carrier at 600-700°C for 6-12h, cooling to 60-80°C and then adding it to 5-8mol / L acetic acid for soaking for 5-10h, and rinsing it with deionized water until the filtrate is neutral to obtain a pretreated carrier; placing the pretreated carrier in trimethylchlorosilane, shaking for 10-30min, standing for 12-20h and then filtering, and drying the obtained carrier to obtain a trimethylchlorosilane-modified porous active carrier.

4. The method for treating marine aquaculture wastewater according to claim 3, characterized in that: The mass ratio of the pretreated carrier to trimethylchlorosilane is (5-8):(9-13).

5. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: The loading in step S2 is to soak the trimethylchlorosilane-modified porous active carrier in the first composite bacterial solution and the second composite bacterial solution respectively, adsorb by shaking for 10 to 15 hours, and then stand for 20 to 25 hours to obtain the first biological carrier and the second biological carrier.

6. The method for treating marine aquaculture wastewater according to claim 5, characterized in that: The trimethylchlorosilane-modified porous active carrier is infiltrated with the first composite bacterial solution and the second composite bacterial solution at a mass ratio of 1: (3-6).

7. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: In step S3, the weight ratio of the first biological carrier to the liquid to be treated is 1:(200-500).

8. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: In step S3, the air aeration time is 3 to 8 hours, the pH of the liquid to be treated is adjusted to 7.0 to 7.5, and the dissolved oxygen of the liquid to be treated is maintained at 1 to 6 mg / L.

9. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: In step S4, the weight ratio of the second biological carrier to the pretreatment liquid is 1:(1000-1500).

10. The method for treating marine aquaculture wastewater according to claim 1, characterized in that: In step S4, the amount of ozone added is 15-25% of the total volume of the pre-treated liquid, and the ozone concentration is 25-50 mg / L.

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