Lutein ester-loaded Pickering emulsion as well as preparation method and application thereof

Abstract

The invention relates to the technical field of natural functional base material delivery systems, in particular to a lutein ester loaded Pickering emulsion as well as a preparation method and application thereof. The lutein ester-loaded Pickering emulsion provided by the invention has relatively high physical and chemical stability, is not easy to layer in a storage process, is relatively low in lutein ester content loss, and has relatively high bioavailability when being applied to an animal body; the emulsion effectively improves the problems of poor water dispersibility, poor storage stability, easy oxidative degradation and low bioavailability of lutein ester. In addition, the lutein ester-loaded Pickering emulsion also has the advantages of simple and efficient preparation method, easiness in industrial production, natural and safe raw materials and the like, and has a better application prospect in the fields of foods, feeds, medicines and the like.

Description

Technical Field

[0001] The invention relates to the technical field of natural functional base material delivery systems, and in particular to a Pickering emulsion loaded with lutein esters, and a preparation method and application thereof. Background Art

[0002] Lutein esters are carotenoids that appear as dark reddish-brown fine particles and are widely found in many plants, such as marigold flowers, pumpkin, cabbage, and spinach. Lutein esters have many beneficial effects on the human body, such as antioxidant effects, which help prevent aging; protect vision and relieve eye fatigue; enhance immunity and have a regulatory effect on cellular immunity. As an important bioactive substance, lutein esters have broad application prospects in functional foods and pharmaceutical products. However, lutein esters are easily affected by oxidation, resulting in reduced activity. In functional foods and pharmaceutical products, the oxidative stability of lutein esters is an important consideration affecting their activity. In addition, the bioavailability of lutein esters is low, mainly due to the hydrophobicity that makes them difficult to dissolve, transport, and absorb, which limits the effective utilization of lutein esters in the body. Moreover, the hydrophobicity of lutein esters makes them difficult to disperse and stabilize in the aqueous phase, which poses a challenge to the preparation of delivery systems for lutein esters.

[0003] Emulsion is an important functional base delivery system. Oil-in-water emulsion (O / W) refers to an emulsion in which oil droplets are suspended in the water phase. The diameter of the oil droplets is usually in the range of 1-100 microns, which can be regulated by the selection and use of emulsifiers. In the water-in-water emulsion, the water phase is the continuous phase and the oil phase is the discrete phase. The oil droplets have a high degree of dispersion and good stability. The oil droplets have a high degree of freedom in the water phase, which is convenient for the release and delivery of drugs. There are many methods for preparing water-in-oil emulsions. The commonly used method is to use mechanical emulsification to mix the oil phase and the water phase through high shear force or high-speed stirring to form an emulsion. Water-in-oil emulsions have broad application prospects in the fields of cosmetics and drug delivery. Water-in-oil emulsions can be used as the basic formula of cosmetics such as emulsions, creams, and emulsion foundations. Its advantages are light texture, easy absorption, and suitable for all skin types. Water-in-oil emulsions are used as drug carriers. By regulating the size and distribution of oil droplets, the stability and delivery efficiency of drugs can be regulated. In addition, oil-in-water emulsions can also prolong the residence time of drugs in the body and improve the bioavailability of drugs. However, the stability of traditional emulsions often requires a large amount of emulsifiers, some emulsifiers are subject to addition restrictions during use, and chemical synthetic emulsifiers may cause harm to the human body, so it is necessary to develop new emulsions.

[0004] The Pickering emulsion delivery system is an emulsion that uses solid particles as stabilizers. The solid particles can be proteins, polysaccharides, polyphenols, etc., or chemically bonded complexes thereof. The raw materials are natural and healthy, and the Pickering emulsion has higher thermodynamic stability. However, the existing Pickering emulsions loaded with lutein esters have problems such as easy stratification, easy loss of content, and the bioavailability still needs to be improved. Therefore, constructing a Pickering emulsion system loaded with lutein esters with excellent physical stability, chemical stability and high bioavailability is a technical bottleneck that needs to be overcome. Summary of the invention

[0005] The invention provides a Pickering emulsion loaded with lutein ester, and a preparation method and application thereof.

[0006] The present invention aims to construct a Pickering emulsion with high stability and bioavailability for lutein esters. During the research and development process, the present invention found that the introduction of glycerol into the aqueous phase system can significantly improve the physical and chemical stability of the Pickering emulsion loaded with lutein esters, and significantly improve the bioavailability of the emulsion. On this basis, the selection and compounding optimization of the oil phase components and solid particles are combined to further improve the above-mentioned effects.

[0007] Specifically, the present invention provides the technical solution described below.

[0008] The present invention provides a Pickering emulsion loaded with lutein ester, the emulsion comprising: an aqueous phase, solid particles and an oil phase; Wherein, the aqueous phase comprises glycerol and water; The oil phase comprises lutein ester, medium chain triglyceride and vegetable oil, and the vegetable oil comprises sunflower oil and palm oil; The solid particles include soy protein isolate and polysaccharide, and the polysaccharide includes sodium alginate and / or chitosan.

[0009] In the Pickering emulsion system of the present invention, composite nanoparticles are formed with soy protein isolate and polysaccharide, lutein ester is dissolved in a medium-chain triglyceride and a specific compound vegetable oil carrier containing a relatively high content of unsaturated fatty acids as the oil phase of the Pickering emulsion, the composite nanoparticles are used as a stabilizer for the Pickering emulsion, a dispersion obtained by mixing the composite nanoparticles with an aqueous phase is mixed with the oil phase, and the Pickering emulsion loaded with lutein ester is formed through emulsification. In the above emulsion system, glycerol can cooperate with the oil phase containing lutein ester, medium-chain triglyceride and the specific compound vegetable oil and the solid particles based on soy protein isolate and polysaccharide, thereby significantly improving the physical and chemical stability of the Pickering emulsion and improving the bioavailability of the emulsion.

[0010] Preferably, the mass percentage of glycerol in the Pickering emulsion is 60-80%. Controlling the amount of glycerol within the above range is more effective in improving the stability and bioavailability of the Pickering emulsion.

[0011] Preferably, in the aqueous phase, the mass ratio of glycerol to water is (2-7):1.

[0012] Preferably, in the oil phase, the mass ratio of medium-chain triglycerides, sunflower seed oil and palm oil is (1-5): (0.5-3): 1. Regarding the selection of vegetable oils, the present invention finds that, based on the introduction of glycerol into the aqueous phase, the compound of sunflower seed oil and palm oil has a better effect on improving the stability and bioavailability of the Pickering emulsion than other vegetable oils. At this time, controlling the mass ratio of medium-chain triglycerides, sunflower seed oil and palm oil within the above range has a better effect on improving the stability and bioavailability of the Pickering emulsion.

[0013] Further preferably, in the oil phase, the mass ratio of medium chain triglycerides, sunflower seed oil and palm oil is (1-4):(0.5-2):1.

[0014] The oil phase described above may also include an oil-soluble antioxidant. Adding an oil-soluble antioxidant can further improve the stability of the lutein ester.

[0015] Preferably, the oil-soluble antioxidant is selected from one or more of vitamin E or its esters, rosemary extract, ascorbyl palmitate, and butylated hydroxytoluene (BHT).

[0016] Preferably, in the solid particles, the mass ratio of soy protein isolate to polysaccharide is (2-12):1.

[0017] Further preferably, in the solid particles, the mass ratio of soy protein isolate to polysaccharide is (2-9):1.

[0018] In some embodiments of the present invention, the solid particles are isolated soy protein and chitosan.

[0019] In other embodiments of the present invention, the solid particles are isolated soy protein and sodium alginate.

[0020] For the above-mentioned aqueous phase into which glycerol is introduced, solid particles composed of soy protein isolate and chitosan or soy protein isolate and sodium alginate have a better effect on the emulsification and stabilization of the lutein ester oil phase.

[0021] Preferably, the mass percentage of lutein ester in the emulsion is 1-10%, more preferably 1-8%.

[0022] Preferably, in the emulsion, the mass ratio of the water phase, the solid particles and the oil phase is (140-200): (0.5-5): (10-40).

[0023] Further preferably, in the emulsion, the mass ratio of the water phase, the solid particles and the oil phase is (150-180): (0.5-3): (10-35).

[0024] In some embodiments of the present invention, the mass percentage of solid particles in the emulsion is 0.2-1.8%.

[0025] The Pickering emulsion loaded with lutein ester provided by the present invention has the advantages of high bioavailability, green safety and high stability, greatly improves the water solubility and stability of lutein ester, and expands the application of lutein ester in the fields of food, animal health products, medicine, etc.

[0026] The present invention also provides a method for preparing the above-mentioned Pickering emulsion loaded with lutein ester, which comprises the step of mixing solid particles with water phase, and after the solid particles are mixed with the water phase, treating the water phase-solid particle mixture at 70-95° C. for 0.5-1.5 h.

[0027] Specifically, the preparation method comprises the following steps: (1) mixing soy protein isolate and polysaccharide with water respectively to obtain soy protein isolate aqueous solution and polysaccharide aqueous solution; (2) adding chitosan aqueous solution to the soy protein isolate aqueous solution, and then adding glycerol to obtain an aqueous phase-solid particle mixture; (3) treating the aqueous phase-solid particle mixture at 70-95°C for 0.5-1.5h, and then cooling to 45-55°C for insulation; (4) mixing the lutein ester with medium chain triglycerides and vegetable oil to obtain an oil phase; (5) After mixing the oil phase of step (4) with the water phase-solid particle mixture of step (3), shear emulsification and homogenization are performed.

[0028] Preferably, in the above step (1), the concentration of the soy protein isolate aqueous solution is (0.5-2.5):20, and the concentration of the polysaccharide aqueous solution is (0.05-0.5):20. The soy protein isolate aqueous solution and the polysaccharide aqueous solution are preferably left overnight.

[0029] In step (2), the chitosan aqueous solution is added while stirring the soy protein isolate aqueous solution, and then glycerol is added, and the mixture is stirred at 180-220 r / min for 7-15 minutes.

[0030] In step (4), the lutein ester crystals are added to the medium chain triglycerides and the vegetable oil, and stirred at 60-70° C. until completely dissolved. If an oil-soluble antioxidant is added, the oil-soluble antioxidant is added after the lutein ester crystals are added to the medium chain triglycerides and the vegetable oil.

[0031] In step (5), the oil phase is added to the aqueous phase-solid particle mixture of step (3). The shear emulsification is preferably performed at 7000-10000 r / min for 4-7 min. The homogenization is preferably performed at 30-100 MPa for 1-3 times. The shear emulsification can be performed using a high-speed shear emulsifier.

[0032] The present invention provides the use of the above-mentioned Pickering emulsion loaded with lutein ester in the preparation of food, feed, medicine or cosmetics.

[0033] The beneficial effects of the present invention include at least: the Pickering emulsion loaded with lutein ester provided by the present invention has high physical and chemical stability, is not easy to stratify during storage, has low loss of lutein ester content, and has high bioavailability when used in animals; the emulsion effectively improves the problems of poor water dispersibility, poor storage stability, easy oxidation degradation and low bioavailability of lutein ester. In addition, the Pickering emulsion loaded with lutein ester of the present invention also has the advantages of simple and efficient preparation method, easy industrial production, natural and safe raw materials, etc., and has good application prospects in the fields of food, feed, medicine, etc. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Example 1

[0036] This embodiment provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is as follows: (1) Dissolve 1.28 g of soy protein isolate in 20 g of water to obtain a soy protein isolate aqueous solution, and dissolve 0.22 g of chitosan in 20 g of water to obtain a chitosan aqueous solution, and leave the solution overnight.

[0037] (2) Add the chitosan aqueous solution while stirring the soy protein isolate aqueous solution, then add 135 g of glycerol, and stir at 200 r / min for 10 min to obtain a complex mixture of soy protein isolate and chitosan (i.e., a water phase-solid particle mixture).

[0038] (3) The mixed solution of the soy protein isolate and chitosan complex was treated at 80°C for 1 h, and then cooled to 50°C and kept warm.

[0039] (4) Add 3.5 g of lutein ester crystals to a composite vegetable oil consisting of 5.6 g of medium-chain triglycerides, 1.4 g of sunflower oil and 1.4 g of palm oil, add 1.6 g of vitamin E, and stir at 68° C. until completely dissolved to prepare an oil phase.

[0040] (5) The oil phase of step (4) was added to the complex mixture of soy protein isolate and chitosan of step (3), mixed and sheared at 8000 r / min for 5 min, and then homogenized twice at 60 MPa to obtain a Pickering emulsion loaded with lutein ester.

[0041] Example 2

[0042] This embodiment provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is as follows: (1) Dissolve 1.28 g of soy protein isolate in 11.5 g of water to obtain a soy protein isolate aqueous solution, and dissolve 0.22 g of sodium alginate in 11.5 g of water to obtain a sodium alginate aqueous solution, and leave the solution overnight.

[0043] (2) Add the sodium alginate aqueous solution while stirring the soy protein isolate aqueous solution, then add 152 g of glycerol, and stir at 200 r / min for 10 min to obtain a complex mixture of soy protein isolate and sodium alginate (i.e., a water phase-solid particle mixture).

[0044] (3) The complex mixture of soy protein isolate and sodium alginate was treated in a water bath at 80°C for 1 hour, and then cooled to 50°C and kept warm.

[0045] (4) Add 3.5 g of lutein ester crystals to a composite vegetable oil consisting of 4.2 g of medium-chain triglycerides, 2.8 g of sunflower oil and 1.4 g of palm oil, add 1.6 g of vitamin E, and stir at 60°C until completely dissolved to prepare an oil phase.

[0046] (5) The oil phase of step (4) was added to the complex mixture of soy protein isolate and sodium alginate of step (3), mixed and sheared at 8000 r / min for 5 min, and then homogenized at 30 MPa twice to obtain a Pickering emulsion loaded with lutein ester.

[0047] Example 3

[0048] This embodiment provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is as follows: (1) Dissolve 1.35 g of soy protein isolate in 20 g of water to obtain a soy protein isolate aqueous solution, and dissolve 0.15 g of sodium alginate in 20 g of water to obtain a sodium alginate aqueous solution, and leave the solution overnight.

[0049] (2) Add the sodium alginate aqueous solution while stirring the soy protein isolate aqueous solution, then add 135 g of glycerol, and stir at 200 r / min for 10 min to obtain a complex mixture of soy protein isolate and sodium alginate (i.e., a water phase-solid particle mixture).

[0050] (3) The complex mixture of soy protein isolate and sodium alginate was treated at 80°C for 1 h, and then cooled to 55°C and kept warm.

[0051] (4) Add 3.5 g of lutein ester crystals to a composite vegetable oil consisting of 2.8 g of medium-chain triglycerides, 2.8 g of sunflower oil, and 2.8 g of palm oil, add 1.6 g of vitamin E, and stir at 68° C. until completely dissolved to prepare an oil phase.

[0052] (5) The oil phase of step (4) was added to the complex mixture of soy protein isolate and sodium alginate of step (3), mixed and sheared at 8000 r / min for 5 min, and then homogenized twice at 100 MPa to obtain a Pickering emulsion loaded with lutein ester.

[0053] Example 4

[0054] This embodiment provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is as follows: (1) Dissolve 1.0 g of soy protein isolate in 20 g of water to obtain a soy protein isolate aqueous solution, and dissolve 0.5 g of chitosan in 20 g of water to obtain a chitosan aqueous solution, and leave the solution overnight.

[0055] (2) Add the chitosan aqueous solution while stirring the soy protein isolate aqueous solution, then add 135 g of glycerol, and stir at 200 r / min for 10 min to obtain a complex mixture of soy protein isolate and chitosan (i.e., a water phase-solid particle mixture).

[0056] (3) The mixed solution of the soy protein isolate and chitosan complex was treated at 80°C for 1 h, and then cooled to 50°C and kept warm.

[0057] (4) Add 3.5 g of lutein ester crystals to a composite vegetable oil consisting of 4.2 g of medium-chain triglycerides, 1.4 g of sunflower oil, and 2.8 g of palm oil, add 1.6 g of vitamin E, and stir at 65° C. until completely dissolved to prepare an oil phase.

[0058] (5) The oil phase of step (4) was added to the complex mixture of soy protein isolate and chitosan of step (3), mixed and sheared at 8000 r / min for 5 min, and then homogenized twice at 60 MPa to obtain a Pickering emulsion loaded with lutein ester.

[0059] Example 5

[0060] This embodiment provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is as follows: (1) Dissolve 0.5 g of soy protein isolate in 20 g of water to obtain a soy protein isolate aqueous solution, and dissolve 0.07 g of chitosan in 20 g of water to obtain a chitosan aqueous solution, and leave the solution overnight.

[0061] (2) Add the chitosan aqueous solution while stirring the soy protein isolate aqueous solution, then add 137.63 g of glycerol, and stir at 200 r / min for 10 min to obtain a complex mixture of soy protein isolate and chitosan (i.e., a water phase-solid particle mixture).

[0062] (3) The complex mixture of soy protein isolate and chitosan was treated at 80°C for 1 hour, and then cooled to 55°C and kept warm.

[0063] (4) Add 3 g of lutein ester crystals to a composite vegetable oil consisting of 3.6 g of medium-chain triglycerides, 1.8 g of sunflower oil, and 1.8 g of palm oil, add 1.6 g of vitamin E, and stir at 65°C until completely dissolved to prepare an oil phase.

[0064] (5) The oil phase of step (4) was added to the complex mixture of soy protein isolate and chitosan of step (3), mixed and sheared at 8000 r / min for 5 min, and then homogenized twice at 45 MPa to obtain a Pickering emulsion loaded with lutein ester.

[0065] Example 6

[0066] This embodiment provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is as follows: (1) Dissolve 2.5 g of soy protein isolate in 20 g of water to obtain a soy protein isolate aqueous solution, and dissolve 0.35 g of chitosan in 20 g of water to obtain a chitosan aqueous solution, and leave the solution overnight.

[0067] (2) Add the chitosan aqueous solution while stirring the soy protein isolate aqueous solution, then add 114 g of glycerol, and stir at 200 r / min for 10 min to obtain a soy protein isolate and chitosan white complex mixture (i.e., a water phase-solid particle mixture).

[0068] (3) The complex mixture of soy protein isolate and chitosan was treated at 80°C for 1 hour, and then cooled to 50°C and kept warm.

[0069] (4) Add 14 g of lutein ester crystals to a composite vegetable oil consisting of 8.8 g of medium-chain triglycerides, 4.4 g of sunflower oil, and 4.4 g of palm oil, add 1.6 g of vitamin E, and stir at 68° C. until completely dissolved to prepare an oil phase.

[0070] (5) The oil phase of step (4) was added to the complex mixture of soy protein isolate and chitosan of step (3), mixed and sheared at 8000 r / min for 5 min, and then homogenized twice at 80 MPa to obtain a Pickering emulsion loaded with lutein ester.

[0071] Comparative Example 1

[0072] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that peanut protein is used instead of soy protein isolate.

[0073] Comparative Example 2

[0074] This comparative example provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is different from that of Example 1 only in that cellulose polysaccharide is used instead of chitosan.

[0075] Comparative Example 3

[0076] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that the total mass of the aqueous phase remains unchanged, but glycerol is not added and is replaced by water.

[0077] Comparative Example 4

[0078] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that: the total mass of the aqueous phase remains unchanged, the amount of glycerol added is adjusted to 104.5 g, and the missing amount is made up with water; the mass percentage of glycerol in the emulsion is 55%.

[0079] Comparative Example 5

[0080] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that: the total mass of the aqueous phase remains unchanged, the amount of glycerol added is adjusted to 161.5 g, and the excess amount is subtracted from the amount of water; the mass percentage of glycerol in the emulsion is 85%.

[0081] Comparative Example 6

[0082] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that sunflower seed oil is used instead of palm oil.

[0083] Comparative Example 7

[0084] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that palm oil is used instead of sunflower oil.

[0085] Comparative Example 8

[0086] This comparative example provides a Pickering emulsion loaded with lutein esters, and the preparation method thereof is different from that of Example 1 only in that sunflower seed oil is used instead of medium-chain triglycerides.

[0087] Comparative Example 9

[0088] This comparative example provides a Pickering emulsion loaded with lutein ester, and the preparation method thereof is different from that of Example 1 only in that the mass ratio of medium chain triglyceride, sunflower seed oil and palm oil is 1:1.5:1.5.

[0089] Experimental Example 1 Storage stability test of emulsion

[0090] The Pickering emulsions loaded with lutein ester prepared by the methods of each embodiment and comparative example were respectively taken, and their states under a microscope were observed, the particle sizes of the emulsions were measured, and the changes in the content of lutein ester in the emulsions were detected after being placed in the dark at 25°C for 4 weeks. The results are shown in Table 1. Among them, the product quality evaluation is the product state after being placed for 4 weeks.

[0091] Table 1 Quality evaluation of lutein ester Pickering emulsion

[0092] Experimental Example 2: Emulsion feeding experiment on laying hens

[0093] The feed containing Pickering emulsion loaded with lutein ester, lutein ester oil solution and lutein ester crystal prepared by the methods of the embodiments and comparative examples was respectively fed to laying hens for 10 days, and the feed intake of laying hens was controlled to be 110 g / day, and the amount of lutein ester added was 40 ppm / day, as the experimental group. The groups without the addition of the above lutein ester products were used as the control group. Three parallel experiments were conducted in each group, and each parallel experiment involved 7 laying hens. The measurement results of the laying hens in each group were averaged.

[0094] The yolk color of the eggs laid by each group of laying hens on the 7th day was measured using the Roche colorimetric fan, and the lutein content in the yolk of the eggs laid by each group of laying hens was detected. The results are shown in Table 2.

[0095] Table 2 Results of feeding experiment on laying hens

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Pickering emulsion loaded with lutein ester, characterized in that: The emulsion comprises: an aqueous phase, solid particles and an oil phase; Wherein, the aqueous phase comprises glycerol and water; The oil phase comprises lutein ester, medium chain triglyceride and vegetable oil, and the vegetable oil comprises sunflower oil and palm oil; The solid particles include soy protein isolate and polysaccharide, and the polysaccharide includes sodium alginate and / or chitosan.

2. The Pickering emulsion loaded with lutein ester according to claim 1, characterized in that: In the Pickering emulsion, the mass percentage of glycerol is 60-80%.

3. The Pickering emulsion loaded with lutein ester according to claim 2, characterized in that: In the aqueous phase, the mass ratio of glycerol to water is (2-7):

1.

4. The Pickering emulsion loaded with lutein ester according to any one of claims 1 to 3, characterized in that: In the oil phase, the mass ratio of medium chain triglycerides, sunflower seed oil and palm oil is (1-5):(0.5-3):

1.

5. The Pickering emulsion loaded with lutein ester according to any one of claims 1 to 4, characterized in that: The oil phase also includes an oil-soluble antioxidant; Preferably, the oil-soluble antioxidant is selected from one or more of vitamin E or its esters, rosemary extract, ascorbyl palmitate, and butylated hydroxytoluene.

6. The Pickering emulsion loaded with lutein ester according to any one of claims 1 to 5, characterized in that: In the solid particles, the mass ratio of soy protein isolate to polysaccharide is (2-12):

1.

7. The Pickering emulsion loaded with lutein ester according to any one of claims 1 to 6, characterized in that: In the emulsion, the mass percentage of lutein ester is 1-10%.

8. The Pickering emulsion loaded with lutein ester according to any one of claims 1 to 7, characterized in that: In the emulsion, the mass ratio of the water phase, the solid particles and the oil phase is (140-200): (0.5-5): (10-40).

9. The method for preparing the Pickering emulsion loaded with lutein ester according to any one of claims 1 to 8, characterized in that: The method comprises: (1) mixing soy protein isolate and polysaccharide with water respectively to obtain soy protein isolate aqueous solution and polysaccharide aqueous solution; (2) adding chitosan aqueous solution to the soy protein isolate aqueous solution, and then adding glycerol to obtain an aqueous phase-solid particle mixed solution; (3) treating the aqueous phase-solid particle mixture at 70-95°C for 0.5-1.5h, and then cooling to 45-55°C for insulation; (4) mixing the lutein ester with medium chain triglycerides and vegetable oil to obtain an oil phase; (5) After mixing the oil phase of step (4) with the water phase-solid particle mixture of step (3), shear emulsification and homogenization are performed.

10. Use of the Pickering emulsion loaded with lutein ester according to any one of claims 1 to 8 in the preparation of food, feed, medicine or cosmetics.

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