Dry-type detection and analysis assembly for blood urea nitrogen and preparation method of dry-type detection and analysis assembly

Abstract

The invention discloses a blood urea nitrogen dry-type detection and analysis assembly and a preparation method thereof.The blood urea nitrogen dry-type detection and analysis assembly comprises an upper shell, a fixed substrate and a lower shell which are sequentially arranged from top to bottom, the upper shell is provided with a sample adding hole, and the lower shell is provided with a urea nitrogen detection hole; a urea nitrogen diffusion layer, a urea nitrogen reaction layer and a urea nitrogen color development layer are sequentially stacked on the fixed substrate from top to bottom, and the urea nitrogen reaction layer comprises urease, a reaction layer adhesive, a stabilizer, a surfactant, a filler, a film-forming agent, a color fixing agent, a defoaming agent and a buffer solution. The urea nitrogen developing layer comprises a developing layer adhesive, a first indicator, a second indicator, a surfactant, a color fixing agent, a film forming agent and a defoaming agent.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a blood urea nitrogen dry-type detection and analysis component and a preparation method thereof. Background Art

[0002] Urea nitrogen detection is widely used in medicine, biochemistry and other fields. In the medical field, urea nitrogen is one of the important indicators of renal function, and the determination of its content is of great significance for the diagnosis and treatment of kidney diseases. In the field of biochemistry, the determination of urea nitrogen can be used to evaluate the nitrogen metabolism in organisms. At present, the detection of urea nitrogen content in whole blood, serum / plasma in humans or animals is mainly carried out by liquid phase kit detection or electrochemical method detection. The above methods have the following problems: the process is cumbersome, the manufacturing cost is high, and the reagents need to be stored at a low temperature of 2-8°C, and the cold chain transportation cost is high. Summary of the invention

[0003] In order to solve the above problems, the present invention provides a blood urea nitrogen dry detection and analysis component and a preparation method thereof.

[0004] The present invention adopts the following technical solutions:

[0005] A blood urea nitrogen dry detection and analysis component comprises an upper shell, a fixed substrate and a lower shell which are arranged in sequence from top to bottom, the upper shell is provided with a sample addition hole, the lower shell is provided with a urea nitrogen detection hole, a urea nitrogen diffusion layer, a urea nitrogen reaction layer and a urea nitrogen color development layer are stacked in sequence from top to bottom on the fixed substrate, the urea nitrogen reaction layer comprises urease, a reaction layer adhesive, a stabilizer, a surfactant, a filler, a film former, a color fixer, a defoamer and a buffer, and the urea nitrogen color development layer comprises a color development layer adhesive, a first indicator, a second indicator, a surfactant, a color fixer, a film former and a defoamer.

[0006] Preferably, the concentrations of the components on the urea nitrogen reaction layer are 10-50 KU / L urease, 0.1-50 g / L reaction layer adhesive, 0.1-10 g / L stabilizer, 0.01-0.1 g / L surfactant, 0.1-1 g / mL filler, 0.001-1 g / mL film-forming agent, 0.0001-0.01 g / mL color fixing agent, 0.001-0.01 g / mL defoaming agent, and 0.01-0.05 M buffer.

[0007] Preferably, the concentrations of the components on the urea nitrogen color-developing layer are 0.1-0.5 g / mL color-developing layer adhesive, 0.005-0.05 g / mL first indicator, 0.0001-0.0005 g / mL second indicator, 0.01-0.1 g / mL surfactant, 0.001-0.01 g / mL color fixing agent, 0.1-1 g / mL film-forming agent, and 0.001-0.01 g / mL defoaming agent.

[0008] Preferably, the stabilizer is one or more of sucrose, EDTA·2Na·2H2O, BSA, reduced glutathione, PEG-2000, PEG-4000, PEG-6000, PEG-8000, trehalose, and mannitol; the filler is one or more of microcrystalline cellulose, bentonite, diatomaceous earth, limestone, and TiO2; the film-forming agent is one or more of hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, chitosan, cyclodextrin, and photographic gelatin; the defoamer is one or more of BYK-020, BYK-021, BYK-022, and BYK-024; the color fixative is betaine; the surfactant is Tween-20, Tween-80, TritonX-100, TritonX-200, TritonX-405, Emulgen B66, one or more of sodium succinate octyl sulfonate; the first indicator is bromothymol blue; the second indicator is phenol red; the buffer is any one of phosphate buffer, TRIS buffer, and MES buffer.

[0009] Preferably, the color development layer adhesive is one or more of D4 hydrogel and D6 hydrogel.

[0010] Preferably, the urea nitrogen diffusion layer is made of mesh.

[0011] Preferably, the fixing substrate is a PEG substrate or a PC substrate.

[0012] Preferably, the steps of preparing the reaction layer adhesive specifically include:

[0013] A1. Weigh acrylamide and N-vinyl pyrrolidone in a mass ratio of 7:3 respectively, place them in 100 g of solvent, stir and dissolve them until they are completely dissolved to form solution a;

[0014] A2. Weigh 1% N,N'-methylenebisacrylamide and 0.5% N,N,N',N'-tetramethylethylenediamine respectively, add them to solution a and stir them evenly to form solution b after emulsification.

[0015] A3. Add solution b into the polymerization reactor and introduce nitrogen for 10 minutes. After the nitrogen is introduced, raise the temperature to 40°C and keep the temperature constant for 30 minutes under the protection of nitrogen. Then add 0.2% potassium persulfate and sodium bisulfite, and continue to react for 10 minutes under constant temperature conditions. Then, react for 4 hours under sealed conditions and take out to obtain the reaction layer adhesive.

[0016] A method for preparing a blood urea nitrogen dry detection and analysis component comprises the following steps:

[0017] S1. Weigh the buffer solution and stabilizer respectively, mix them, adjust the pH value, stir for 0.5 h, add the surfactant, filler, film former, color fixative, defoamer, urease, and reaction layer adhesive, and continue stirring for 1.5 h to obtain a urea nitrogen reaction solution;

[0018] S2, weigh the display layer adhesive, the first indicator, the second indicator, and the surfactant respectively, mix and stir, stir for 0.5 h, add the color fixing agent, the film forming agent, and the defoaming agent, and continue stirring for 0.5-2 h to obtain a urea nitrogen color developing solution;

[0019] S3, fix the fixed substrate on the coating machine, set the coating thickness to 100-500 mm, the coating rate to 10-1000 cm / min, coat the urea nitrogen color developing solution on the fixed substrate, transfer to 40-80° C. environment for drying for 1-10 min, and form a urea nitrogen color developing layer after drying;

[0020] S4, the fixed substrate with the urea nitrogen color-developing layer after drying is continued to be fixed on the coating machine, and the coating thickness is set to 100-500mm, the coating rate is 10-1000cm / min, the urea nitrogen reaction solution is coated on the dried urea nitrogen color-developing layer, and after coating, it is transferred to a 40-80°C environment for drying for 5-30min, and a urea nitrogen reaction layer is formed after drying;

[0021] S5. The urea nitrogen diffusion layer, the urea nitrogen reaction layer, the urea nitrogen color development layer and the fixed substrate are stacked in the lower shell from top to bottom according to the stacking order, and then the upper shell cover is arranged on the upper part of the lower shell to form a blood urea nitrogen dry detection and analysis component.

[0022] The detection principle of the dry nitrogen detection and analysis component of the present invention is:

[0023]

[0024] NH3 + acid-base indicator → indicator changes color

[0025] After adopting the above technical solution, the present invention has the following advantages compared with the background technology:

[0026] The present invention provides a blood urea nitrogen dry detection and analysis component and a preparation method thereof. The dry detection and analysis component is made into a dry detection and analysis component through dry chemical coating and is used in conjunction with a dry biochemical analyzer to quickly and quantitatively detect the content of urea nitrogen in whole blood, serum / plasma in human or animal bodies. It is efficient and convenient and meets the needs of instant diagnosis and on-site diagnosis. In addition, the dry chemical coating process is simple, low in cost, easy to carry, can be stored at room temperature, has stable performance, and reduces storage and transportation costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A flow chart for preparing a blood urea nitrogen dry type detection and analysis assembly of the present invention;

[0028] Figure 2 This is a linear correlation diagram of the blood urea nitrogen dry type detection and analysis component and the test results of the biochemical analyzer method in Example 2 of the present invention;

[0029] Figure 3 This is a linear correlation diagram of the blood urea nitrogen dry type detection and analysis component and the test results of the biochemical analyzer method in Example 3 of the present invention;

[0030] Figure 4 This is a linear correlation diagram of the blood urea nitrogen dry detection and analysis component and the test results of the biochemical analyzer method in Example 4 of the present invention. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0032] Example 1

[0033] like Figure 1 As shown, the present invention discloses a blood urea nitrogen dry detection and analysis component, comprising an upper shell, a fixed substrate and a lower shell arranged in sequence from top to bottom, the upper shell is provided with a sample addition hole, the lower shell is provided with a urea nitrogen detection hole, a urea nitrogen diffusion layer, a urea nitrogen reaction layer and a urea nitrogen color development layer are stacked in sequence from top to bottom on the fixed substrate, the urea nitrogen reaction layer comprises urease, a reaction layer adhesive, a stabilizer, a surfactant, a filler, a film-forming agent, a color fixing agent, a defoaming agent and a buffer, and the urea nitrogen color development layer comprises a color development layer adhesive, a first indicator, a second indicator, a surfactant, a color fixing agent, a film-forming agent and a defoaming agent.

[0034] The concentrations of the components on the urea nitrogen reaction layer are 10-50 KU / L urease, 0.1-50 g / L reaction layer adhesive, 0.1-10 g / L stabilizer, 0.01-0.1 g / L surfactant, 0.1-1 g / mL filler, 0.001-1 g / mL film former, 0.0001-0.01 g / mL color fixative, 0.001-0.01 g / mL defoamer, and 0.01-0.05 M buffer.

[0035] The concentrations of the components on the urea nitrogen color development layer are 0.1-0.5 g / mL color development layer adhesive, 0.005-0.05 g / mL first indicator, 0.0001-0.0005 g / mL second indicator, 0.01-0.1 g / mL surfactant, 0.001-0.01 g / mL color fixing agent, 0.1-1 g / mL film former, and 0.001-0.01 g / mL defoaming agent.

[0036] The stabilizer is one or more of sucrose, EDTA·2Na·2H2O (disodium ethylenediaminetetraacetic acid dihydrate), BSA (bovine serum albumin), reduced glutathione, PEG-2000, PEG-4000, PEG-6000, PEG-8000, trehalose, and mannitol; the filler is one or more of microcrystalline cellulose, bentonite, diatomaceous earth, limestone, and TiO2; the film-forming agent is one or more of hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, chitosan, cyclodextrin, and photographic gelatin; the defoamer is one or more of BYK-020, BYK-021, BYK-022, and BYK-024; the color fixative is betaine; the surfactant is Tween-20, Tween-80, TritonX-100, TritonX-200, Triton X-405, Emulgen B66, one or more of sodium succinate octyl sulfonate; the first indicator is bromothymol blue; the second indicator is phenol red; the buffer is any one of phosphate buffer, TRIS buffer, and MES buffer.

[0037] The color developing layer adhesive is one or more of D4 hydrogel and D6 hydrogel.

[0038] The urea nitrogen diffusion layer is made of mesh cloth, and the fixed substrate is made of PEG substrate or PC substrate.

[0039] The preparation steps of the reaction layer adhesive specifically include:

[0040] A1. Weigh acrylamide and N-vinyl pyrrolidone in a mass ratio of 7:3 respectively, place them in 100 g of solvent, stir and dissolve them until they are completely dissolved to form solution a;

[0041] A2. Weigh 1% N,N'-methylenebisacrylamide and 0.5% N,N,N',N'-tetramethylethylenediamine respectively, add them to solution a and stir them evenly to form solution b after emulsification.

[0042] A3. Add solution b into a polymerization reactor and introduce nitrogen for 10 minutes. After the nitrogen is introduced, raise the temperature to 40°C and keep the temperature constant for 30 minutes under the protection of nitrogen. Then add 0.2% potassium persulfate and sodium bisulfite, and continue to react for 10 minutes under constant temperature conditions. Then, react for 4 hours under sealed conditions and take out to obtain a reaction layer adhesive (poly(acrylamide-N-vinyl pyrrolidone)).

[0043] The detection principle of the blood urea nitrogen dry detection and analysis component of the present invention is:

[0044]

[0045] NH3 + acid-base indicator → indicator changes color

[0046] Example 2

[0047] like Figure 1 As shown, the present invention discloses a method for preparing a blood urea nitrogen dry detection and analysis component, comprising the following steps:

[0048] S1. Weigh the buffer solution and stabilizer respectively, mix them, and adjust the pH value to 6.0. Use a spiral stirrer at a stirring speed of 700 r / min. After stirring for 0.5 h, add a surfactant, a filler, a film-forming agent, a color fixing agent, a defoaming agent, urease, and a reaction layer adhesive. Continue stirring for 1.5 h at a stirring speed of 700 r / min to obtain a urea nitrogen reaction solution.

[0049] S2, respectively weigh the display layer adhesive, the first indicator, the second indicator, and the surfactant, mix and stir at a stirring speed of 400 r / min, stir for 0.5 h, add the color fixing agent, the film-forming agent, and the defoaming agent, continue stirring for 0.5-2 h, and stir at a stirring speed of 400 r / min to obtain a urea nitrogen color developing solution;

[0050] The concentrations of the components of the urea nitrogen reaction solution are 10-50 KU / L urease, 0.1-50 g / L reaction layer adhesive, 0.1-10 g / L stabilizer, 0.01-0.1 g / L surfactant, 0.1-1 g / mL filler, 0.001-1 g / mL film former, 0.0001-0.01 g / mL color fixative, 0.001-0.01 g / mL defoamer, and 0.01-0.05 M buffer.

[0051] The concentrations of the components of the urea nitrogen color development solution are 0.1-0.5 g / mL color development layer binder, 0.005-0.05 g / mL first indicator, 0.0001-0.0005 g / mL second indicator, 0.01-0.1 g / mL surfactant, 0.001-0.01 g / mL color fixative, 0.1-1 g / mL film former, 0.001-0.01 g / mL defoamer.

[0052] S3, fix the fixed substrate on the coating machine, set the coating thickness to 100-500 mm, the coating rate to 60 cm / min, coat the urea nitrogen color developing solution on the fixed substrate, transfer to 70°C environment for drying for 1-10 min after coating, and after drying, the coating reaches 50 μm to form a urea nitrogen color developing layer;

[0053] S4, the fixed substrate with the urea nitrogen color-developing layer after drying is continued to be fixed on the coating machine, and the coating thickness is set to 100-500mm, the coating rate is 60cm / min, the urea nitrogen reaction solution is coated on the dried urea nitrogen color-developing layer, and after coating, it is transferred to a 70°C environment for drying for 5-30min, and a urea nitrogen reaction layer is formed after drying, and the total coating thickness reaches 200μm;

[0054] S5. Cut the fixed substrate with urea nitrogen reaction layer, urea nitrogen color development layer and urea nitrogen diffusion layer into 100mm 2 The reagent card is stacked in the lower shell from top to bottom according to the stacking order of the urea nitrogen diffusion layer, the urea nitrogen reaction layer, the urea nitrogen color development layer and the fixed substrate, and then the upper shell cover is arranged on the upper part of the lower shell to assemble and form a blood urea nitrogen dry detection and analysis component.

[0055] The composition ratios of the urea nitrogen reaction solution and the urea nitrogen color developing solution in this embodiment are shown in Table 1 and Table 2 respectively:

[0056] Table 1: Component ratio of urea nitrogen reaction solution:

[0057] Reagents <![CDATA[Coating amount / m 2 > PBS PH6.0 PEG-2000 2g Trehalose 5g Carboxymethyl cellulose 10g Tween-20 5g High carbon alcohol defoamer 5mL BSA 200mg Gypsum powder 20g Urease 20KU Reaction layer adhesive 10g

[0058] Table 2: Composition ratio of urea nitrogen colorimetric solution:

[0059] Reagents <![CDATA[Coating amount / m 2 > D4 Hydrogel 20g Bromothymol blue 1.5g Cellulose powder 25g High carbon alcohol defoamer 1mL Betaine 2g

[0060] This implementation uses 20 serum samples for verification, and the correlation of multiple test samples is analyzed by the blood urea nitrogen dry type detection and analysis component of this embodiment and the biochemical analyzer test method. Figure 2 As shown, the test results of the blood urea nitrogen dry type detection and analysis component of this embodiment and the test results of the biochemical analyzer method show a good linear correlation.

[0061] Example 3

[0062] like Figure 1 As shown, the present invention discloses a method for preparing a blood urea nitrogen dry detection and analysis component, comprising the following steps:

[0063] S1. Weigh the buffer solution and stabilizer respectively, mix them, and adjust the pH value to 6.2. Use a spiral stirrer at a stirring speed of 800 r / min. After stirring for 0.5 h, add a surfactant, a filler, a film-forming agent, a color fixing agent, a defoaming agent, urease, and a reaction layer adhesive. Continue stirring for 1.5 h at a stirring speed of 800 r / min to obtain a urea nitrogen reaction solution.

[0064] S2, respectively weigh the display layer adhesive, the first indicator, the second indicator, and the surfactant, mix and stir at a stirring speed of 500 r / min, stir for 0.5 h, add the color fixing agent, the film-forming agent, and the defoaming agent, continue stirring for 0.5-2 h, and stir at a stirring speed of 500 r / min to obtain a urea nitrogen color developing solution;

[0065] The concentrations of the components of the urea nitrogen reaction solution are 10-50 KU / L urease, 0.1-50 g / L reaction layer adhesive, 0.1-10 g / L stabilizer, 0.01-0.1 g / L surfactant, 0.1-1 g / mL filler, 0.001-1 g / mL film former, 0.0001-0.01 g / mL color fixative, 0.001-0.01 g / mL defoamer, and 0.01-0.05 M buffer.

[0066] The concentrations of the components of the urea nitrogen color development solution are 0.1-0.5 g / mL color development layer binder, 0.005-0.05 g / mL first indicator, 0.0001-0.0005 g / mL second indicator, 0.01-0.1 g / mL surfactant, 0.001-0.01 g / mL color fixative, 0.1-1 g / mL film former, 0.001-0.01 g / mL defoamer.

[0067] S3, fix the fixed substrate on the coating machine, set the coating thickness to 100-500mm, the coating rate to 70cm / min, coat the urea nitrogen color developing solution on the fixed substrate, transfer to 70°C environment for drying for 1-10min after coating, and after drying, the coating reaches 60μm to form a urea nitrogen color developing layer;

[0068] S4, the fixed substrate with the urea nitrogen color-developing layer after drying is continued to be fixed on the coating machine, and the coating thickness is set to 100-500mm, the coating rate is 70cm / min, the urea nitrogen reaction solution is coated on the dried urea nitrogen color-developing layer, and after coating, it is transferred to a 70°C environment for drying for 5-30min, and a urea nitrogen reaction layer is formed after drying, and the total coating thickness reaches 220μm;

[0069] S5. Cut the fixed substrate with urea nitrogen reaction layer, urea nitrogen color development layer and urea nitrogen diffusion layer into 100mm 2 The reagent card is stacked in the lower shell from top to bottom according to the stacking order of the urea nitrogen diffusion layer, the urea nitrogen reaction layer, the urea nitrogen color development layer and the fixed substrate, and then the upper shell cover is arranged on the upper part of the lower shell to assemble and form a blood urea nitrogen dry detection and analysis component.

[0070] The composition ratios of the urea nitrogen reaction solution and the urea nitrogen color developing solution in this embodiment are shown in Table 3 and Table 4 respectively:

[0071] Table 13: Component ratio of urea nitrogen reaction solution:

[0072] Reagents <![CDATA[Coating amount / m 2 > PBS PH6.2 PEG-4000 3g sucrose 4g Benzyl cellulose 5g TritonX-405 3g High carbon alcohol defoamer 5mL BSA 500mg <![CDATA[TiO2]]> 10g Urease 20KU Reaction layer adhesive 15g

[0073] Table 4: Composition ratio of urea nitrogen colorimetric solution:

[0074] Reagents <![CDATA[Coating amount / m 2 > D4 Hydrogel 20g Bromothymol blue 1.0g limestone 20g High carbon alcohol defoamer 2mL Betaine 1g

[0075] This implementation uses 20 serum samples for verification, and the correlation of multiple test samples is analyzed by the blood urea nitrogen dry type detection and analysis component of this embodiment and the biochemical analyzer test method. Figure 3 As shown, the test results of the blood urea nitrogen dry type detection and analysis component of this embodiment and the test results of the biochemical analyzer method show a good linear correlation.

[0076] Example 4

[0077] like Figure 1 As shown, the present invention discloses a method for preparing a blood urea nitrogen dry detection and analysis component, comprising the following steps:

[0078] S1. Weigh the buffer solution and stabilizer respectively, mix them, and adjust the pH value to 6.3. Use a spiral stirrer at a stirring speed of 500 r / min. After stirring for 0.5 h, add a surfactant, a filler, a film-forming agent, a color fixing agent, a defoaming agent, urease, and a reaction layer adhesive. Continue stirring for 1.5 h at a stirring speed of 500 r / min to obtain a urea nitrogen reaction solution.

[0079] S2, respectively weigh the display layer adhesive, the first indicator, the second indicator, and the surfactant, mix and stir at a stirring speed of 500 r / min, stir for 0.5 h, add the color fixing agent, the film-forming agent, and the defoaming agent, continue stirring for 0.5-2 h, and stir at a stirring speed of 500 r / min to obtain a urea nitrogen color developing solution;

[0080] The concentrations of the components of the urea nitrogen reaction solution are 10-50 KU / L urease, 0.1-50 g / L reaction layer adhesive, 0.1-10 g / L stabilizer, 0.01-0.1 g / L surfactant, 0.1-1 g / mL filler, 0.001-1 g / mL film former, 0.0001-0.01 g / mL color fixative, 0.001-0.01 g / mL defoamer, and 0.01-0.05 M buffer.

[0081] The concentrations of the components of the urea nitrogen color development solution are 0.1-0.5 g / mL color development layer binder, 0.005-0.05 g / mL first indicator, 0.0001-0.0005 g / mL second indicator, 0.01-0.1 g / mL surfactant, 0.001-0.01 g / mL color fixative, 0.1-1 g / mL film former, 0.001-0.01 g / mL defoamer.

[0082] S3, fix the fixed substrate on the coating machine, set the coating thickness to 100-500 mm, the coating rate to 80 cm / min, coat the urea nitrogen color developing solution on the fixed substrate, transfer to 60°C environment for drying for 1-10 min after the coating is completed, and after the drying is completed, the coating reaches 80 μm to form a urea nitrogen color developing layer;

[0083] S4, the fixed substrate with the urea nitrogen color-developing layer after drying is continued to be fixed on the coating machine, and the coating thickness is set to 100-500 mm, the coating rate is 80 cm / min, the urea nitrogen reaction solution is coated on the dried urea nitrogen color-developing layer, and after coating, it is transferred to a 60° C. environment for drying for 5-30 minutes, and a urea nitrogen reaction layer is formed after drying, and the total coating thickness reaches 250 μm;

[0084] S5. Cut the fixed substrate with urea nitrogen reaction layer, urea nitrogen color development layer and urea nitrogen diffusion layer into 100mm 2 The reagent card is stacked in the lower shell from top to bottom according to the stacking order of the urea nitrogen diffusion layer, the urea nitrogen reaction layer, the urea nitrogen color development layer and the fixed substrate, and then the upper shell cover is arranged on the upper part of the lower shell to assemble and form a blood urea nitrogen dry detection and analysis component.

[0085] The composition ratios of the urea nitrogen reaction solution and the urea nitrogen color developing solution in this embodiment are shown in Table 5 and Table 6 respectively:

[0086] Table 5: Component ratio of urea nitrogen reaction solution:

[0087] Reagents <![CDATA[Coating amount / m 2 > PBS PH6.3 PEG-2000 5g sucrose 4g Hydroxypropyl Cellulose 10g TritonX-200 6g High carbon alcohol defoamer 2mL Trehalose 5g Bentonite 26g Urease 22KU Reaction layer adhesive 15g

[0088] Table 6: Composition ratio of urea nitrogen color developing solution:

[0089] Reagents <![CDATA[Coating amount / m 2 > D6 Hydrogel 30g Bromothymol blue 2.0g Bentonite 20g High carbon alcohol defoamer 3mL Betaine 1.5g

[0090] This implementation uses 20 serum samples for verification, and the correlation of multiple test samples is analyzed by the blood urea nitrogen dry type detection and analysis component of this embodiment and the biochemical analyzer test method. Figure 4 As shown, the test results of the blood urea nitrogen dry type detection and analysis component of this embodiment and the test results of the biochemical analyzer method show a good linear correlation.

[0091] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A blood urea nitrogen dry type detection and analysis component, characterized in that: The invention comprises an upper shell, a fixed substrate and a lower shell which are arranged in sequence from top to bottom, the upper shell is provided with a sample addition hole, the lower shell is provided with a urea nitrogen detection hole, a urea nitrogen diffusion layer, a urea nitrogen reaction layer and a urea nitrogen color development layer are stacked in sequence from top to bottom on the fixed substrate, the urea nitrogen reaction layer comprises urease, a reaction layer adhesive, a stabilizer, a surfactant, a filler, a film-forming agent, a color fixing agent, a defoaming agent and a buffer, and the urea nitrogen color development layer comprises a color development layer adhesive, a first indicator, a second indicator, a surfactant, a color fixing agent, a film-forming agent and a defoaming agent.

2. A blood urea nitrogen dry type detection and analysis component as claimed in claim 1, characterized in that: The concentrations of the components on the urea nitrogen reaction layer are respectively 10-50 KU / L urease, 0.1-50 g / L reaction layer adhesive, 0.1-10 g / L stabilizer, 0.01-0.1 g / L surfactant, 0.1-1 g / mL filler, 0.001-1 g / mL film former, 0.0001-0.01 g / mL color fixative, 0.001-0.01 g / mL defoamer, and 0.01-0.05 M buffer.

3. A blood urea nitrogen dry type detection and analysis component as claimed in claim 1, characterized in that: The concentrations of the components on the urea nitrogen color-developing layer are respectively 0.1-0.5 g / mL of a color-developing layer adhesive, 0.005-0.05 g / mL of a first indicator, 0.0001-0.0005 g / mL of a second indicator, 0.01-0.1 g / mL of a surfactant, 0.001-0.01 g / mL of a color-fixing agent, 0.1-1 g / mL of a film-forming agent, and 0.001-0.01 g / mL of a defoaming agent.

4. A blood urea nitrogen dry type detection and analysis component as claimed in claim 1, characterized in that: The stabilizer is one or more of sucrose, EDTA·2Na·2H2O, BSA, reduced glutathione, PEG-2000, PEG-4000, PEG-6000, PEG-8000, trehalose, and mannitol; the filler is one or more of microcrystalline cellulose, bentonite, diatomaceous earth, limestone, and TiO2; the film-forming agent is one or more of hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, chitosan, cyclodextrin, and photographic gelatin; the defoamer is one or more of BYK-020, BYK-021, BYK-022, and BYK-024; the color fixative is betaine; the surfactant is Tween-20, Tween-80, TritonX-100, TritonX-200, TritonX-405, Emulgen B66, one or more of sodium succinate octyl sulfonate; the first indicator is bromothymol blue; the second indicator is phenol red; the buffer is any one of phosphate buffer, TRIS buffer, and MES buffer.

5. The blood urea nitrogen dry type detection and analysis component according to claim 1, characterized in that: The color development layer adhesive is one or more of D4 hydrogel and D6 hydrogel.

6. A blood urea nitrogen dry type detection and analysis component as claimed in claim 1, characterized in that: The urea nitrogen diffusion layer is made of mesh cloth.

7. A blood urea nitrogen dry type detection and analysis component as claimed in claim 1, characterized in that: The fixed substrate is a PEG substrate or a PC substrate.

8. The blood urea nitrogen dry type detection and analysis component according to claim 1, characterized in that: The steps for preparing the reaction layer adhesive specifically include: A1. Weigh acrylamide and N-vinyl pyrrolidone in a mass ratio of 7:3 respectively, place them in 100 g of solvent, stir and dissolve them until they are completely dissolved to form solution a; A2. Weigh 1% N,N'-methylenebisacrylamide and 0.5% N,N,N',N'-tetramethylethylenediamine respectively, add them to solution a and stir them evenly to form solution b after emulsification. A3. Add solution b into the polymerization reactor and introduce nitrogen for 10 minutes. After the nitrogen is introduced, raise the temperature to 40°C and keep the temperature constant for 30 minutes under the protection of nitrogen. Then add 0.2% potassium persulfate and sodium bisulfite, and continue to react for 10 minutes under constant temperature conditions. Then, react for 4 hours under sealed conditions and take out to obtain the reaction layer adhesive.

9. A method for preparing the blood urea nitrogen dry detection and analysis component according to claims 1-8, characterized in that: The following steps are involved: S1. Weigh the buffer solution and stabilizer respectively, mix them, adjust the pH value, stir for 0.5 h, add the surfactant, filler, film former, color fixative, defoamer, urease, and reaction layer adhesive, and continue stirring for 1.5 h to obtain a urea nitrogen reaction solution; S2, weigh the display layer adhesive, the first indicator, the second indicator, and the surfactant respectively, mix and stir, stir for 0.5 h, add the color fixing agent, the film forming agent, and the defoaming agent, and continue stirring for 0.5-2 h to obtain a urea nitrogen color developing solution; S3, fix the fixed substrate on the coating machine, set the coating thickness to 100-500 mm, the coating rate to 10-1000 cm / min, coat the urea nitrogen color developing solution on the fixed substrate, transfer to 40-80° C. environment for drying for 1-10 min, and form a urea nitrogen color developing layer after drying; S4, the fixed substrate with the urea nitrogen color-developing layer after drying is continued to be fixed on the coating machine, and the coating thickness is set to 100-500mm, the coating rate is 10-1000cm / min, the urea nitrogen reaction solution is coated on the dried urea nitrogen color-developing layer, and after coating, it is transferred to a 40-80°C environment for drying for 5-30min, and a urea nitrogen reaction layer is formed after drying; S5. The urea nitrogen diffusion layer, the urea nitrogen reaction layer, the urea nitrogen color development layer and the fixed substrate are stacked in the lower shell from top to bottom according to the stacking order, and then the upper shell cover is arranged on the upper part of the lower shell to form a blood urea nitrogen dry detection and analysis component.

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