Compound preparation for promoting tobacco root growth and application thereof

Abstract

The invention provides a compound preparation for promoting tobacco root growth and application thereof, and belongs to the technical field of tobacco planting.The compound preparation is prepared by mixing 1 * 10 < 6 >-1 * 10 < 11 > ensifer adhesion and 3-5 g of silafeng, the compound preparation is safe and free of pollution and can be applied from tobacco seedling raising to the resettling stage, and the application mode is root irrigation. The compound medicament obviously promotes the growth of tobacco roots, improves the root dry weight, the fresh weight, the longest root length, the root surface area, the root volume, the root activity and the like, and is beneficial to improving the tobacco quality.

Description

Technical Field

[0001] The invention belongs to the technical field of tobacco planting, and in particular relates to a compound preparation for promoting tobacco root growth and application thereof. Background Art

[0002] The tobacco root system is one of the important organs of tobacco. It determines the absorption of soil nutrients and water by tobacco and is the main source of water and mineral nutrition required by tobacco plants. The tobacco root system initially develops shallowly, then extends downward and can reach up to 2 meters underground. The tobacco root system is mainly concentrated in loam and clay layers because these soils can accommodate more water and nutrients. In addition, the tobacco root system is dispersed and has branch roots, which can reach deeper soil and absorb nutrients and water. The tobacco root system is also an important organ for tobacco to absorb water and nutrients from the soil and synthesize hormones and alkaloids. The size, distribution and vitality of the tobacco root system largely determine the amount of nutrients absorbed by the tobacco plant. The quality of the tobacco root system development will directly affect the appearance and growth of the tobacco plant, and then affect the yield, quality and intrinsic quality of the tobacco.

[0003] In the process of tobacco growth and quality formation, in order to promote root growth, traditional fertilization is to use a large amount of fertilizer to promote growth, which not only pollutes the soil environment, but also easily leads to an imbalance in the mineral nutrition balance of the tobacco field soil, resulting in a decline in the quality of tobacco leaves; currently, there are reports of using trace elements as fertilizers for tobacco, but the fertilizers are generally complex in composition, unclear in fertilizer efficiency, low in absorption rate, and high in cost, which is not conducive to promotion and application. Plant rhizosphere growth-promoting bacteria are a type of beneficial microorganisms that can promote plant growth and its absorption and utilization of mineral nutrients, and can inhibit harmful organisms. They live freely in the soil or epiphyte on the roots of plants. They can promote plant growth, reduce the use of chemical fertilizers, and can also reduce the application of pesticides to a certain extent, playing the role of "weight loss and drug reduction". Therefore, it is imperative to develop a compound preparation with simple ingredients, good fertilizer efficiency, good for tobacco absorption, and no environmental pollution. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a compound preparation for promoting the growth of tobacco root system and its application, which can significantly promote the growth of tobacco root system and improve the quality of tobacco.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a compound preparation for promoting the growth of tobacco root system, including sticky sword fungus and silicon ring;

[0007] The ratio of the sticky sword fungus to the silicon-rich ring is: every 1×10 6 ~1×10 11 Each sticky sword fungus: 3 to 5g of silicon ring.

[0008] Preferably, the deposit number of the sticky sword fungus is CGMCC No.28087.

[0009] Preferably, the sticky sword fungus is used in the form of a bacterial liquid, and the concentration of live bacteria in the sticky sword fungus liquid is 1×10 7 ~1×10 10 cfu / mL, and the mass ratio of the sticky sword fungus liquid to the silicon-rich ring is 2-5:3-5.

[0010] Preferably, the preparation method of the compound preparation is to mix the bacterial liquid of the sticky sword fungus with silicon ring.

[0011] The invention also provides application of the compound preparation in tobacco planting.

[0012] Preferably, the compound preparation is diluted 500 to 2000 times with water before use.

[0013] Preferably, the compound preparation is used from the tobacco seedling stage to the bush stage.

[0014] Preferably, the compound preparation is used by root irrigation.

[0015] Preferably, the usage of the compound preparation is 20 to 80 mL / mu.

[0016] The invention also provides application of the compound preparation in promoting the growth of tobacco root system and improving tobacco quality.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The compound preparation prepared by the present invention is composed of sticky sword fungus and silicon-rich ring, and the raw materials are safe, non-toxic to humans and livestock, non-toxic to crops, non-polluting to the environment, and non-irritating to the skin and eyes, and resistant to rain erosion. The present invention is the first to discover the new use of sticky sword fungus in promoting the growth of tobacco roots, and at the same time uses silicon-rich ring to induce the division of crop seed cells, especially the mitosis of rooting cells and the enhancement of protein biosynthesis ability, thereby promoting the growth of tobacco roots. The continuous compounding can significantly promote the growth of tobacco roots, and all the root-related parameters are improved, which indirectly improves the disease resistance of tobacco roots and reduces the occurrence of diseases. DETAILED DESCRIPTION

[0019] The invention provides a compound preparation for promoting the growth of tobacco root system, including sticky sword fungus and silicon ring;

[0020] The ratio of the sticky sword fungus to the silicon-rich ring is: every 1×10 6 ~1×10 11 Each sticky sword fungus: 3 to 5g of silicon ring.

[0021] In the present invention, the ratio of the sticky sword fungus to the silicon-rich ring is 1×10 6 ~1×10 11 Adhesive sword fungus: 3-5g silicon ring, preferably 1×10 7 ~1×10 10 Adhesive sword fungus: 3.5-4.5 g of silicon-rich ring, more preferably 3×10 8 Each sticky sword fungus: 4g silicon ring.

[0022] In the present invention, the sticky sword fungus is preferably used in the form of a bacterial liquid. The preparation method of the sticky sword fungus bacterial liquid is as follows: preferably, the sticky sword fungus is streaked and activated on an LB solid culture medium plate, and after purification, a single colony is picked and transferred to an LB liquid culture medium for culture. The culture time is preferably 16 to 32 hours, more preferably 20 to 28 hours, and more preferably 24 hours; preferably, the bacteria are collected by centrifugation, and the centrifugal speed is preferably 8000 to 12000 rpm, more preferably 9000 to 11000 rpm, and more preferably 10000 rpm. The centrifugal time is preferably 1 to 3 minutes, and more preferably 2 minutes. The bacteria are added to sterile physiological saline and mixed evenly to obtain a bacterial suspension. The OD of the bacterial suspension is 600 is preferably 0.5-0.9, more preferably 0.6-0.8, and more preferably 0.7. The bacterial suspension is added to LB liquid culture medium for cultivation. The volume ratio of the bacterial suspension to the LB liquid culture medium is preferably 1-5 mL:100 mL, more preferably 2-4 mL:100 mL, and more preferably 3 mL:100 mL. The culture temperature is preferably 30-34 ° C, more preferably 31-33 ° C, and more preferably 32 ° C. The shaking speed is preferably 100-180 r / min, more preferably 120-160 r / min, and more preferably 140 r / min. The culture time is preferably 36-60 h, more preferably 40-54 h, and more preferably 48 h to obtain the sticky sword fungus liquid. The live bacteria concentration of the sticky sword fungus liquid is preferably 1×10 7 ~1×10 10 cfu / mL, more preferably 1×10 8 ~5×10 9 cfu / mL, more preferably 1×10 9 cfu / mL, the mass ratio of the sticky sword fungus liquid to the silicon-rich ring is preferably 2-5:3-5, more preferably 2.5-4:3.5-4.5, and further preferably 3:4; the preparation method of the composite preparation is preferably to mix the sticky sword fungus liquid with the silicon-rich ring.

[0023] The invention also provides application of the compound preparation in tobacco planting.

[0024] In the present invention, the compound preparation is diluted with water before use, and the volume of water used is preferably 500 to 2000 times the volume of the compound preparation, more preferably 800 to 1500 times, and further preferably 1000 times; the use time of the compound preparation is preferably from the tobacco seedling stage to the cluster stage; the use method of the compound preparation is preferably root irrigation, and the usage amount is preferably 20 to 80 mL / mu, more preferably 40 to 60 mL / mu, and further preferably 50 mL / mu.

[0025] The present invention also provides the use of the compound preparation in promoting the growth of tobacco root system and improving tobacco quality. The compound preparation can significantly promote the growth of tobacco root system.

[0026] Butyl emulsifiable concentrate was purchased from Dagong Agrochemical Co., Ltd., Zhangye City, Gansu Province;

[0027] The sticky sword fungus comes from patent CN202311369544.0 and was donated by the laboratory to which the patent belongs;

[0028] Silicon Fenghuan was purchased from Liaoning Shanshui Yinong Technology Co., Ltd.

[0029] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0030] Example 1 The promoting effect of compound agent on tobacco root system during seedling raising period

[0031] 1.1 Preparation of compound preparations

[0032] The sticky sword fungus was streaked on an LB solid medium plate for activation culture. After purification, a single colony was picked and transferred to LB liquid medium for culture for 24 hours. The bacteria were centrifuged to obtain the bacteria. The bacteria were added to sterile physiological saline and mixed evenly to obtain a bacterial suspension with an OD 600 of 0.7. The bacterial suspension was added to LB liquid medium at a volume ratio of 3mL:100mL and cultured at 32°C and 140r / min for 48 hours to ensure that the number of viable bacteria in the sticky sword fungus culture liquid was not less than 1×10 8 cfu / mL, and obtain the bacterial solution of Sword fungus. Mix the bacterial solution of Sword fungus and silicon ring in a mass ratio of 3:4 to obtain the composite preparation.

[0033] 1.2 Experimental Grouping

[0034] According to different experimental materials, they are divided into different experimental groups, including:

[0035] Compound preparation group: dilute the compound preparation prepared in (1.1) with water. Dilute 2 ml of compound preparation with 1 L of water and spray evenly on the surface of the seedling medium. Spray 50 ml per tray.

[0036] Silicon-free ring group: dilute 2 ml of 50% Silicon-free ring wet seed dressing with 1L of water and spray evenly on the surface of the seedling substrate. Spray 50ml per tray. Sow the seeds in the conventional production method. Spray water evenly to saturate the substrate and maintain a thickness of about 3cm. Cover with soil after sowing. The thickness of the soil is about 0.1cm. Cover with film to keep warm and moisturize.

[0037] Adhesive Sword fungus liquid group: dilute the Adhesive Sword fungus liquid prepared in (1.1) with water, dilute every 2 ml of Adhesive Sword fungus liquid with 1 L of water and spray evenly on the surface of the seedling medium, spray 50 ml per tray, sow seeds in accordance with conventional production methods, spray water evenly to saturate the water content of the medium and maintain a thickness of about 3 cm, cover with soil after sowing, the thickness of the soil is about 0.1 cm, and cover with a film to keep warm and moisturize after covering with moist soil;

[0038] Butralin EC group: 48% Butralin EC was diluted with water, and 5 ml of 48% Butralin EC was diluted with 1 L of water and evenly sprayed on the surface of the seedling medium. 50 ml was sprayed on each plate. Seedlings were raised and sown according to the conventional production method. Water was evenly sprayed to make the water content of the medium reach a saturated state and maintain a thickness of about 3 cm. After sowing, the soil was covered with a thickness of about 0.1 cm, and the film was covered with moisture to keep warm and moisturize.

[0039] Clear water was used as the control group (CK): sterile water was sprayed evenly on the surface of the seedling matrix, 50 ml was sprayed on each tray, and seedlings were sown according to conventional production methods. Water was sprayed evenly to saturate the matrix with water and maintain a thickness of about 3 cm. After sowing, the soil was covered with a thickness of about 0.1 cm. After the soil was moistened, a film was covered to keep warm and moisturize.

[0040] The seeds used for seedling raising and sowing are: tobacco: K326.

[0041] 1.3 Agronomic traits survey

[0042] Samples were taken 50 days after emergence to determine root fresh weight, root dry weight, root-shoot ratio, longest root length, root surface area, root volume and root activity.

[0043] The fresh weight and dry weight of the whole plant and root system were measured by weighing method, and the root-to-shoot ratio was calculated; the root surface area and root volume were measured by methylene blue method; and the root activity was measured by triphenyltetrazolium chloride reduction method (TTC method). The results are shown in Table 1.

[0044] Table 1 Survey results

[0045]

[0046] As can be seen from Table 1, the seedlings treated with the compound agent have significantly improved root weight, length, surface area, volume and root activity compared with the clear water control. At the same time, compared with the use of silicon ring and sticky sword fungus solution alone, the effect of the compound agent is better, and the growth-promoting effect on the roots of tobacco seedlings is higher than that of the positive control, butarin.

[0047] Example 2 Effect of compound preparation on the growth of tobacco root system during transplanting period

[0048] The test site was in Luzhou City, Sichuan Province. The test plots were randomly arranged and repeated 5 times. Each plot had 45 tobacco plants and an area of ​​20m2. 2 . Irrigate the roots when transplanting, and the spraying amount is 50mL / mu.

[0049] Agronomic traits investigation: Root fresh weight, root dry weight, root-shoot ratio, longest root length, root surface area, root volume and root activity were measured after 28 days of cultivation.

[0050] The fresh weight and dry weight of the whole plant and root system were measured by weighing method, and the root-to-shoot ratio was calculated; the root surface area and root volume were measured by methylene blue method; and the root activity was measured by triphenyltetrazolium chloride reduction method (TTC method). The results are shown in Table 2.

[0051] Table 2 Survey results

[0052]

[0053] The root parameters after the transplanting period are obtained from Table 2. It can be seen that the use of the compound agent provided by the present invention can greatly promote the root growth of tobacco plants, and each value is higher than that of the tobacco in the group treated with chlorfenapyr. The above parameters are important agronomic traits for the growth of tobacco roots and are one of the important ways to preliminarily judge the quality of tobacco plants. It can be seen that the compound agent prepared by the present invention can effectively promote the root growth of tobacco plants and improve the quality of tobacco leaves. And because the product obtained by the present invention is a compound preparation, it will not pollute the environment when used, and has the characteristics of safety, no toxic side effects on humans and livestock, and no phytotoxic effects on crops. Therefore, it can be used as a tobacco root growth regulator for in-depth research.

[0054] Example 3 Synergistic Effect of Silicon-rich Ring and Adhesive Sword Fungus Liquid

[0055] The concentrations of silanol and sticky sword fungus liquid that produce the same effect as the compound drug when applied alone were measured, and according to the drug efficacy synergy evaluation formula, it was obtained that the two components in the compound drug have a synergistic relationship. The evaluation is: CI = Ac / Ae+Bc / Be, where Ac and Bc represent the concentrations of drug A and drug B used in combination, respectively; Ae and Be represent the concentrations of drug A and drug B that produce the same effect when administered alone. If CI < 1, the drugs are considered to have a synergistic effect.

[0056] The experimental results in Example 2 of this patent were used for evaluation. The compound concentration is: every 2 ml of the compound preparation is diluted with 1 L of water, the mass ratio of the sticky sword fungus liquid to the silicon-rich ring is 3:4, then every 1 L of water contains about 0.86 mL of sticky sword fungus and 1.14 mL of silicon-rich ring, and the root activity is 164.56 μg / (g·h); when the root activity is the same, the concentration of silicon-rich ring applied alone is 2.89 ml / 1 L of water; the concentration of sticky sword fungus liquid applied alone is 3.12 ml / 1 L of water, and the following table is obtained using the evaluation formula:

[0057] Table 3 Synergistic effect of silicon-rich ring and sticky sword fungus liquid

[0058]

[0059] As shown in Table 3, CI = 0.64 < 1, indicating that the silica-rich ring and the sticky sword fungus liquid have a synergistic effect, and it is not a simple functional superposition.

[0060] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A compound preparation for promoting tobacco root growth, characterized in that: Includes sticky sword fungi and silicon-rich rings; The ratio of the sticky sword fungus to the silicon-rich ring is: every 1×10 6 ~1×10 11 Each sticky sword fungus: 3 to 5g of silicon ring.

2. The compound preparation according to claim 1, characterized in that The deposit number of the sticky sword fungus is CGMCC No.28087.

3. The compound preparation according to claim 1, characterized in that The sticky sword fungus is used in the form of a bacterial liquid, and the live bacteria concentration of the sticky sword fungus liquid is 1×10 7 ~1×10 10 cfu / mL, and the mass ratio of the sticky sword fungus liquid to the silicon-rich ring is 2-5:3-5.

4. The compound preparation according to claim 3, characterized in that: The preparation method of the compound preparation is to mix the bacterial liquid of the sticky sword fungus with silicon ring.

5. Use of the compound preparation according to any one of claims 1 to 4 in tobacco planting.

6. The use according to claim 5, characterized in that: The compound preparation is diluted 500 to 2000 times with water before use.

7. The use according to claim 5, characterized in that: The compound preparation can be used from the tobacco seedling stage to the tobacco bush stage.

8. The use according to claim 5, characterized in that: The use of the compound preparation includes root irrigation.

9. The use according to claim 5, characterized in that: The usage amount of the compound preparation is 20 to 80 mL / mu.

10. Use of the compound preparation according to any one of claims 1 to 4 in promoting tobacco root growth and improving tobacco quality.

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