Preparation method of emulsion for increasing survival rate of young hippocampus

Abstract

The invention discloses a preparation method of an emulsion for increasing the survival rate of young hippocampus, which comprises the following steps: S100: carrying out wall breaking treatment on a purple perilla seed raw material to obtain a purple perilla seed wall-broken material; s200, mixing the wall-broken purple perilla seed material with water, and then performing vacuum cooking treatment to obtain a purple perilla seed mixture; s300, performing supercritical extraction on the purple perilla seed mixture to obtain a purple perilla seed extract; and S400, mixing the purple perilla seed extraction material with a beta-cyclodextrin / Arabic gum mixed solution, and then carrying out emulsification and homogenization treatment, so as to obtain the purple perilla seed oil Pickering emulsion. The emulsion prepared by the method not only can strengthen the artemia at a lower cost, but also can remarkably improve the survival rate of artificially cultured Hippocampus kelloggi fries by the strengthened artemia.

Description

Technical Field

[0001] The invention relates to the technical field of seahorse seedling cultivation, and in particular to a method for preparing an emulsion for improving the survival rate of seahorse seedlings. Background Art

[0002] Seahorse is a traditional precious Chinese medicinal material. Due to the decline of natural seahorse populations in my country, breeding has become the only way to replace fishing. American line seahorse is a species of seahorse breeding that has emerged in recent years, accounting for about 10% of my country's seahorse breeding market and has huge economic value. However, the biggest problem currently encountered in the breeding of American line seahorse is the low survival rate of seedlings.

[0003] Highly unsaturated fatty acids are essential nutrients for the growth and development of seahorse seedlings, and play an irreplaceable role in enhancing the immunity and growth of seahorse seedlings. In order to meet the demand for highly unsaturated fatty acids during the growth of seahorse seedlings, Artemia is currently commonly used as a biological bait for seahorse seedlings, but the ω-3 polyunsaturated fatty acids contained in Artemia cannot meet the growth needs of seahorse seedlings.

[0004] In order to solve this problem, the commonly used method is to fortify Artemia to meet the production needs in marine aquaculture. At present, Artemia mainly adopts nutritional fortification methods such as microalgae, oil-type fortifiers, and microcapsule fortifiers. However, after conducting relevant research on many aquatic species, it was found that these fortification methods have strong species specificity and are not suitable for application in the cultivation of seahorse seedlings. Moreover, the cost of emulsified fish oil and 50DE-microcapsules, which have good fortification effects on seahorse larvae, is too high and is not suitable for large-scale promotion in production. Therefore, finding a low-cost Artemia fortification method for significantly improving the survival rate of artificially cultured American line seahorse seedlings is an urgent issue to be solved. Summary of the invention

[0005] The invention provides a method for preparing an emulsion for improving the survival rate of seahorse seedlings. The emulsion prepared by the method can not only strengthen Artemia at a lower cost, but also the strengthened Artemia can significantly improve the survival rate of artificially cultured American lineated seahorse seedlings.

[0006] The technical solution adopted by the present invention to solve the above technical problems is: a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0007] S100: breaking the wall of the perilla seed raw material to obtain a perilla seed broken wall material;

[0008] S200: mixing the perilla seed wall-broken material with water, and then performing vacuum cooking to obtain a perilla seed mixed material;

[0009] S300: performing supercritical extraction on the perilla seed mixture to obtain a perilla seed extract;

[0010] S400: mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution, and then performing emulsification and homogenization treatment to obtain a perilla seed oil Pickering emulsion.

[0011] Furthermore, S100 specifically includes the following steps:

[0012] Take a perilla seed raw material, first perform microwave drying on the perilla seed raw material, and then perform a wall breaking treatment to obtain a perilla seed wall-breaking material with a wall-breaking particle size of 40 meshes to 80 meshes.

[0013] Furthermore, S200 specifically includes the following steps:

[0014] The perilla seed wall-breaking material is mixed with water in a mass ratio of 1:(0.8-1.2), and then vacuum-cooked under sealed conditions, and the cooking temperature is controlled at 50°C-60°C, the cooking time is controlled at 15min-25min, and the vacuum degree is controlled at 0.03MPa-0.07MPa to obtain a perilla seed mixture.

[0015] Further, in S300, the extraction pressure of the supercritical extraction is controlled at 35MPa-40MPa, the extraction temperature is controlled at 35°C-45°C, the separation pressure is controlled at 8MPa-10MPa, the separation temperature is controlled at 45°C-55°C, and the extraction time is controlled at 5h-7h.

[0016] Furthermore, in S300, the yield of the perilla seed extract obtained after supercritical extraction is controlled to be 32%-36%.

[0017] Further, in S400, the β-cyclodextrin / gum arabic mixed solution is prepared by the following method:

[0018] S411: mixing β-cyclodextrin and distilled water in a mass ratio of (1-3):100, and then homogenizing at a speed of 8000 r / min-12000 r / min for 45 s-75 s, and then stirring with a magnetic stirrer until the β-cyclodextrin is completely dissolved in the distilled water to obtain a β-cyclodextrin solution;

[0019] S412: mixing gum arabic and distilled water in a mass ratio of (1-3):100, and then homogenizing at a speed of 8000 r / min-12000 r / min for 45 s-75 s, and then stirring with a magnetic stirrer until the gum arabic is completely dissolved in the distilled water to obtain a gum arabic solution;

[0020] S413: Mixing a β-cyclodextrin solution and a gum arabic solution in a mass ratio of 1:(0.8-1.2), and then concentrating to obtain a β-cyclodextrin / gum arabic mixed solution.

[0021] Further, in S413, the volume ratio of the mixed β-cyclodextrin solution and the gum arabic solution to the volume of the β-cyclodextrin / gum arabic mixed solution is 20:(17-19).

[0022] Further, in S400, the perilla seed extract in a mass ratio of (1.9-2.1):(17-19) is mixed with a β-cyclodextrin / gum arabic mixed solution, and the rotation speed of the emulsification homogenization treatment is controlled at 12000r / min-18000r / min and the treatment time is controlled at 4min-6min, and finally a perilla seed oil Pickering emulsion is obtained.

[0023] Further, in S400, after the perilla seed extract is subjected to embedding treatment, it is mixed with the β-cyclodextrin / gum arabic mixed solution, wherein the specific steps of the embedding treatment are as follows:

[0024] S510: mixing maltodextrin, soy protein, seaweed polysaccharide, and pure water in a mass ratio of (2-3):(3-4):(0.9-0.11):(690-710) to obtain a first mixture;

[0025] S520: mixing the perilla seed extract with the first mixed material in a mass ratio of (3-4):5 to obtain a second mixed material;

[0026] S530: spray-drying the second mixed material to obtain an embedded perilla seed extract.

[0027] Further, in S510, maltodextrin, soy protein, seaweed polysaccharide, and pure water are homogenized at a speed of 13000 r / min-15000 r / min for 60 s-90 s to obtain a first mixture;

[0028] In S520, the perilla seed extract and the first mixed material are homogenized at a speed of 14000 r / min-16000 r / min for 2 min-3 min to obtain a second mixed material in an emulsion state.

[0029] Compared with the prior art, the advantages of the present invention are:

[0030] (1) The emulsion prepared by the present invention uses perilla seeds as the main raw material, and the content of α-linolenic acid in the extracted perilla seed oil is as high as 60%, which can be used as an important source of ω-3 polyunsaturated fatty acids and can effectively strengthen Artemia;

[0031] (2) The Pickering emulsion of perilla seed oil finally prepared by the present invention has good stability and dispersibility, and the preparation process is simple, the cost is low, and it can be used in a variety of environments;

[0032] (3) The perilla oil extraction process used in the present invention has better extraction efficiency than the traditional process and does not increase the cost of the extraction process;

[0033] (4) The present invention also performs an embedding treatment on the perilla seed oil. Such a treatment can further improve the stability of the perilla seed oil Pickering emulsion during transportation and storage, thereby ensuring the quality of the product. DETAILED DESCRIPTION

[0034] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, specific embodiments of the present invention are described in detail below.

[0035] The embodiment of the present invention provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0036] S100: breaking the wall of the perilla seed raw material to obtain a perilla seed broken wall material;

[0037] S200: mixing the perilla seed wall-broken material with water, and then performing vacuum cooking to obtain a perilla seed mixed material;

[0038] S300: performing supercritical extraction on the perilla seed mixture to obtain a perilla seed extract;

[0039] S400: mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution, and then performing emulsification and homogenization treatment to obtain a perilla seed oil Pickering emulsion.

[0040] Among them, in S100, the perilla seeds purchased from the market are firstly cleaned and screened, and the perilla seeds with better quality are used as the perilla seed raw materials. Then, the perilla seed raw materials are microwave-dried at a temperature of 85°C, and then the perilla seed raw materials are wall-broken by a wall-breaking machine, so as to finally obtain the perilla seed wall-broken material with a wall-broken particle size of 40 mesh to 80 mesh.

[0041] Microwave drying of the perilla seed raw material can, on the one hand, remove moisture from the perilla seed raw material, and on the other hand, make the surface of the perilla seed brittle, thereby reducing the difficulty of the wall breaking process.

[0042] Preferably, the perilla seed wall-breaking material has a wall-breaking particle size of 50 meshes. Under this particle size condition, not only can the loss of perilla seed oil during the wall-breaking process be reduced, but the perilla seeds can also be wall-broken as much as possible.

[0043] After the perilla seeds are subjected to the wall-breaking treatment, in order to improve the extraction efficiency of the perilla seed oil, the perilla seed wall-breaking material is subjected to low-temperature vacuum cooking before extraction. S200 specifically includes the following steps:

[0044] The perilla seed wall-breaking material is mixed with water in a mass ratio of 1:(0.8-1.2), and then vacuum-cooked under sealed conditions, and the cooking temperature is controlled at 50°C-60°C, the cooking time is controlled at 15min-25min, and the vacuum degree is controlled at 0.03MPa-0.07MPa to obtain a perilla seed mixture.

[0045] Preferably, the perilla seed wall-breaking material is mixed with water in a mass ratio of 1:1.

[0046] Preferably, the cooking temperature is controlled at 55° C., the cooking time is controlled at 20 min, and the vacuum degree is controlled at 0.05 MPa to obtain a perilla seed mixture.

[0047] After low-temperature vacuum cooking, the perilla seed mixture is subjected to supercritical extraction, and the perilla seed extract obtained by supercritical extraction has perilla seed oil as its main component, wherein the main steps S300 of supercritical extraction are as follows: controlling the extraction pressure of supercritical extraction at 35MPa-40MPa, the extraction temperature at 35℃-45℃, the separation pressure at 8MPa-10MPa, the separation temperature at 45℃-55℃, and the extraction time at 5h-7h.

[0048] Preferably, the extraction pressure of supercritical extraction is controlled at 38 MPa, the extraction temperature is controlled at 40° C., the separation pressure is controlled at 9 MPa, the separation temperature is controlled at 50° C., and the extraction time is controlled at 6 h.

[0049] After low-temperature vacuum cooking and supercritical extraction, the final yield of perilla seed extract is 32%-36%. Compared with direct supercritical extraction of perilla seed wall-broken material, the extraction yield is greatly improved.

[0050] After the perilla seed oil is extracted, the perilla seed oil is prepared into a perilla seed oil Pickering emulsion. Compared with directly using the perilla seed oil, the perilla seed oil Pickering emulsion can be well dispersed in water, avoiding the phenomenon of perilla seed oil agglomerating and floating in water.

[0051] The preparation of the perilla seed oil Pickering emulsion mainly comprises mixing the perilla seed extract (that is, the perilla seed oil) with a β-cyclodextrin / arabic gum mixed solution, and then performing an emulsification and homogenization treatment. In actual operation, the perilla seed extract with a mass ratio of (1.9-2.1): (17-19) is mixed with the β-cyclodextrin / arabic gum mixed solution, and the speed of the emulsification and homogenization treatment is controlled at 12000r / min-18000r / min and the treatment time is controlled at 4min-6min, and finally the perilla seed oil Pickering emulsion is obtained.

[0052] Preferably, the perilla seed extract is mixed with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and the speed of the emulsification homogenization treatment is controlled at 15000 r / min and the treatment time is 5 min, so as to finally obtain the perilla seed oil Pickering emulsion.

[0053] The β-cyclodextrin / gum arabic mixed solution used is prepared by the following method:

[0054] S411: mixing β-cyclodextrin and distilled water in a mass ratio of (1-3):100, and then homogenizing at a speed of 8000 r / min-12000 r / min for 45 s-75 s, and then stirring with a magnetic stirrer until the β-cyclodextrin is completely dissolved in the distilled water to obtain a β-cyclodextrin solution;

[0055] S412: mixing gum arabic and distilled water in a mass ratio of (1-3):100, and then homogenizing at a speed of 8000 r / min-12000 r / min for 45 s-75 s, and then stirring with a magnetic stirrer until the gum arabic is completely dissolved in the distilled water to obtain a gum arabic solution;

[0056] S413: Mixing a β-cyclodextrin solution and a gum arabic solution in a mass ratio of 1:(0.8-1.2), and then concentrating to obtain a β-cyclodextrin / gum arabic mixed solution.

[0057] Preferably, in S411, β-cyclodextrin and distilled water are mixed in a mass ratio of 2:100, and then homogenized at a speed of 10000 r / min for 60 s.

[0058] Preferably, in S412, gum arabic and distilled water are mixed in a mass ratio of 2:100, and then homogenized at a speed of 10000 r / min for 60 s.

[0059] Preferably, in S413, the β-cyclodextrin solution and the gum arabic solution are mixed in a mass ratio of 1:1.

[0060] Wherein, in S413, the volume ratio of the mixed volume of the β-cyclodextrin solution and the gum arabic solution to the volume of the β-cyclodextrin / gum arabic mixed solution is 20:(17-19).

[0061] Preferably, the volume ratio of the mixed volume of the β-cyclodextrin solution and the gum arabic solution to the volume of the β-cyclodextrin / gum arabic mixed solution is 20:18.

[0062] In order to prevent the agglomeration and uneven distribution of the perilla seed oil in the perilla seed oil Pickering emulsion during transportation and storage, the perilla seed oil may be embedded before the emulsification and homogenization of the perilla seed oil. The specific steps of the embedding treatment are as follows:

[0063] S510: maltodextrin, soy protein, seaweed polysaccharide, and pure water are mixed in a mass ratio of (2-3): (3-4): (0.9-0.11): (690-710), and homogenized at a speed of 13000 r / min-15000 r / min for 60 s-90 s to obtain a first mixture;

[0064] S520: mixing the perilla seed extract in a mass ratio of (3-4):5 with the first mixed material, and homogenizing at a speed of 14000 r / min-16000 r / min for 2 min-3 min to obtain a second mixed material;

[0065] S530: spray-drying the second mixed material to obtain an embedded perilla seed extract.

[0066] Preferably, in S510, maltodextrin, soy protein, seaweed polysaccharide and pure water are mixed in a mass ratio of 2.3:3.7:1:700, and homogenized at a speed of 14000 r / min for 75 seconds to obtain a first mixture.

[0067] Preferably, in S520: the perilla seed extract is mixed with the first mixed material in a mass ratio of 3.5:5, and homogenized at a speed of 15000 r / min for 2.5 min to obtain a second mixed material.

[0068] Wherein, in the second mixed material, the perilla extract is suspended in the first mixed material in the form of particles with a diameter of 0.5um-0.8um.

[0069] Before spray drying the second mixture, in order to ensure the stable formation of particles, it is necessary to add 15wt% hydrochloric acid solution while stirring at 50°C until the pH value of the second mixture reaches 4.5, and then perform the spray drying step.

[0070] The spray drying controlled feed rate was 300mL / h-450mL / h, the inlet temperature was 105℃-115℃, and the air volume was 60m 3 / h-70m 3 / h, the outlet temperature is 70℃-80℃.

[0071] Preferably, the spray drying is controlled to have a feed rate of 380 mL / h, an inlet temperature of 110°C, and an air volume of 65 m 3 / h, the outlet temperature is 75℃.

[0072] In order to ensure the ultimate use effect of the perilla seed oil Pickering emulsion, a mixture of perilla seed extract and encapsulated perilla seed extract is also used.

[0073] Preferably, the perilla seed extract, the embedded perilla seed extract and the β-cyclodextrin / gum arabic mixed solution are mixed in a mass ratio of 1.4:0.6:18, and then emulsified and homogenized to obtain the perilla seed oil Pickering emulsion.

[0074] Preferably, the perilla seed extract, the embedded perilla seed extract and the β-cyclodextrin / gum arabic mixed solution are mixed in a mass ratio of 0.6:1.4:18, and then emulsified and homogenized to obtain the perilla seed oil Pickering emulsion.

[0075] In order to ensure the final use effect of the perilla seed oil Pickering emulsion, an auxiliary agent can also be added to the perilla seed extract or the embedded perilla seed extract. The auxiliary agent is prepared by the following method:

[0076] The residue remaining after extraction in S300 is taken out, washed with pure water and air-dried at 85°C, and then ultrafinely crushed with a grinder to obtain a residue powder with a particle size of 150um-180um; then the residue powder and distilled water are mixed in a mass ratio of 10:200, and then homogenized at a speed of 13000r / min for 1min to obtain an auxiliary agent.

[0077] When in use, the perilla seed extract (or embedded perilla seed extract), the auxiliary agent and the β-cyclodextrin / arabic gum mixed solution are mixed in a mass ratio of 2:1:18.

[0078] Embodiment 1:

[0079] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0080] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0081] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0082] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0083] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0084] Embodiment 2:

[0085] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0086] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 60 meshes;

[0087] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0088] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0089] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0090] Embodiment three:

[0091] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0092] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 40 meshes;

[0093] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0094] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0095] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0096] The perilla seed extracts (i.e., perilla seed oil) prepared in Examples 1 to 3 were weighed respectively, and then the yields were calculated. The yield of the perilla seed extract in Example 1 was 35.8%, the yield of the perilla seed extract in Example 2 was 32.5%, and the yield of the perilla seed extract in Example 3 was 34.9%. By comparison, it can be seen that when the perilla seed wall-breaking material with a wall-breaking particle size of 50 mesh is selected, a higher yield is obtained, that is, the acquisition rate of perilla seed oil is higher.

[0097] Embodiment 4:

[0098] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0099] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0100] S200: directly subjecting the perilla seed wall-broken material to supercritical extraction, controlling the extraction pressure of the supercritical extraction to 38 MPa, the extraction temperature to 40° C., the separation pressure to 9 MPa, the separation temperature to 50° C., and the extraction time to 6 h, to obtain a perilla seed extract;

[0101] S300: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0102] The perilla seed extract obtained in Example 4 was weighed, and the yield of the perilla seed extract was calculated to be 29.2%. By comparing the yields of the perilla seed extract in Example 1 and Example 4, it can be seen that the step of low-temperature vacuum cooking of the perilla seed wall-breaking material in Example 1 can effectively improve the yield of the perilla seed extract, thereby effectively improving the utilization rate of the raw materials and reducing the overall process cost.

[0103] Embodiment five:

[0104] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0105] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0106] S200: mixing the perilla seed wall-broken material with water in a mass ratio of 1:0.9, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to 55° C., the cooking time to 20 min, and the vacuum degree to 0.05 MPa to obtain a perilla seed mixture;

[0107] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0108] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0109] Embodiment five:

[0110] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0111] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0112] S200: mixing the perilla seed wall-broken material with water in a mass ratio of 1:1.1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0113] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0114] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0115] The perilla seed extracts prepared in Example 4 and Example 5 were weighed respectively, and then the yields were calculated. The yield of the perilla seed extract in Example 4 was 35.5%, and the yield of the perilla seed extract in Example 5 was 35.6%. By comparing Example 1, Example 4, and Example 5, it can be seen that in S200, when the perilla seed wall-breaking material and water are mixed in a ratio of 1:1, a better yield of the perilla seed extract is achieved.

[0116] Embodiment six:

[0117] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0118] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0119] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 15 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0120] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0121] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0122] Embodiment seven:

[0123] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0124] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0125] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature at 55° C., the cooking time at 25 min, and the vacuum degree at 0.05 MPa to obtain a perilla seed mixture;

[0126] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0127] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:18, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0128] The perilla seed extracts prepared in Example 6 and Example 7 were weighed respectively, and then the yields were calculated. The yield of the perilla seed extract in Example 6 was 34.3%, and the yield of the perilla seed extract in Example 7 was 35.6%. By comparing Example 1, Example 6, and Example 7, it can be seen that in S200, the time of low-temperature vacuum cooking has a certain effect on the yield of the perilla seed extract. The longer the cooking time, the higher the yield. However, there is no difference in the yield between cooking for 20 minutes and cooking for 25 minutes. It can be seen that cooking for 20 minutes can better save energy consumption.

[0129] Embodiment eight:

[0130] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0131] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0132] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0133] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0134] S400: Mixing the perilla seed extract with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 1:10, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0135] Embodiment nine:

[0136] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0137] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0138] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0139] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0140] S400: Mixing the perilla seed extract with a mass ratio of 2.1:17 with the β-cyclodextrin / gum arabic mixed solution, and controlling the speed of the emulsification homogenization treatment to 15000 r / min and the treatment time to 5 min, finally obtaining a perilla seed oil Pickering emulsion.

[0141] The Perilla seed oil Pickering emulsions prepared in Example 1, Example 8 and Example 9 were left to stand and observed in a sealed environment. It was found that after 45 days, the Perilla seed oil Pickering emulsion of Example 1 was very stable without agglomeration and stratification, the Perilla seed oil Pickering emulsion of Example 8 had a slight stratification at the bottom, and the Perilla seed oil Pickering emulsion of Example 8 had a slight agglomeration on the upper layer.

[0142] Embodiment ten:

[0143] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0144] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0145] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0146] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0147] S400: Mix maltodextrin, soy protein, seaweed polysaccharide and pure water in a mass ratio of 2.3:3.7:1:700, and homogenize at a speed of 14000r / min for 75s to obtain a first mixture; then mix the perilla seed extract in a mass ratio of 3.5:5 with the first mixture, and homogenize at a speed of 15000r / min for 2.5min to obtain a second mixture; then spray-dry the second mixture to obtain an embedded perilla seed extract; mix the embedded perilla seed extract in a mass ratio of 2:18 with a β-cyclodextrin / gum arabic mixed solution, and control the speed of the emulsification homogenization treatment at 15000r / min and the treatment time at 5min to finally obtain a perilla seed oil Pickering emulsion.

[0148] Embodiment eleven:

[0149] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0150] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0151] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0152] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0153] S400: maltodextrin, soy protein, seaweed polysaccharide and pure water in a mass ratio of 2.3:3.7:1:700 are mixed, and homogenized at a speed of 14000r / min for 75s to obtain a first mixture; then a perilla seed extract in a mass ratio of 3.5:5 is mixed with the first mixture, and homogenized at a speed of 15000r / min for 2.5min to obtain a second mixture; the second mixture is stirred while adding a 15wt% hydrochloric acid solution dropwise to the mixture at a temperature of 50°C until the pH value of the second mixture reaches 4.5, and then spray-dried to obtain an embedded perilla seed extract; the embedded perilla seed extract in a mass ratio of 2:18 is mixed with a β-cyclodextrin / gum arabic mixed solution, and the speed of the emulsification homogenization treatment is controlled at 15000r / min and the treatment time is controlled at 5min to finally obtain a perilla seed oil Pickering emulsion.

[0154] The Perilla seed oil Pickering emulsions prepared in Example 1, Example 10 and Example 11 were left to stand and observed in a sealed environment. It was found that after 45 days, the Perilla seed oil Pickering emulsions of Example 1, Example 10 and Example 11 were very stable without any agglomeration or stratification. After 90 days, the Perilla seed oil Pickering emulsion of Example 1 had a bottom seal layer and agglomeration of the upper Perilla seed oil, the Perilla seed oil Pickering emulsion of Example 10 had slight agglomeration of the Perilla seed oil, and the Perilla seed oil Pickering emulsion of Example 11 was very stable without any stratification or agglomeration.

[0155] Embodiment 12:

[0156] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0157] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0158] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0159] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0160] S400: maltodextrin, soy protein, seaweed polysaccharide and pure water in a mass ratio of 2.3:3.7:1:700 are mixed, and homogenized at a speed of 14000r / min for 75s to obtain a first mixture; then a perilla seed extract in a mass ratio of 3.5:5 is mixed with the first mixture, and homogenized at a speed of 15000r / min for 2.5min to obtain a second mixture; the second mixture is stirred while adding a 15wt% hydrochloric acid solution dropwise to the mixture at a temperature of 50°C until the pH value of the second mixture reaches 4.5, and then spray-dried to obtain an embedded perilla seed extract; perilla seed extract, embedded perilla seed extract and β-cyclodextrin / gum arabic mixed solution in a mass ratio of 1.4:0.6:18 are mixed, and the speed of the emulsification homogenization treatment is controlled at 15000r / min and the treatment time is controlled at 5min to finally obtain a perilla seed oil Pickering emulsion.

[0161] Embodiment 13:

[0162] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0163] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0164] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0165] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0166] S400: maltodextrin, soy protein, seaweed polysaccharide and pure water in a mass ratio of 2.3:3.7:1:700 are mixed, and homogenized at a speed of 14000r / min for 75s to obtain a first mixture; then a perilla seed extract in a mass ratio of 3.5:5 is mixed with the first mixture, and homogenized at a speed of 15000r / min for 2.5min to obtain a second mixture; the second mixture is stirred while adding a 15wt% hydrochloric acid solution dropwise to the mixture at a temperature of 50°C until the pH value of the second mixture reaches 4.5, and then spray-dried to obtain an embedded perilla seed extract; perilla seed extract, embedded perilla seed extract and β-cyclodextrin / gum arabic mixed solution in a mass ratio of 0.6:1.4:18 are mixed, and the speed of the emulsification homogenization treatment is controlled at 15000r / min and the treatment time is controlled at 5min to finally obtain a perilla seed oil Pickering emulsion.

[0167] The Perilla seed oil Pickering emulsions prepared in Example 11, Example 12 and Example 13 were left to stand and observed in a sealed environment. It was found that after 45 days, the Perilla seed oil Pickering emulsions of Example 11, Example 12 and Example 13 were all very stable, without agglomeration or stratification. After 90 days, the Perilla seed oil Pickering emulsion of Example 12 had a slight bottom seal and agglomeration of the upper layer of Perilla seed oil, but the phenomenon was not obvious. The Perilla seed oil Pickering emulsions of Example 11 and Example 13 were all very stable, without agglomeration or stratification.

[0168] Embodiment 14:

[0169] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0170] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0171] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0172] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0173] S400: The residue remaining after extraction in S300 is taken out, washed with pure water and air-dried at 85°C, and then ultrafinely crushed with a grinder to obtain a residue powder with a particle size of 150um-180um; then the residue powder and distilled water are mixed in a mass ratio of 10:200, and then homogenized at a speed of 13000r / min for 1min to obtain an auxiliary agent; the perilla seed extract, the auxiliary agent and the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:1:18 are mixed, and the speed of the emulsification homogenization treatment is controlled at 15000r / min and the treatment time is controlled at 5min to finally obtain a perilla seed oil Pickering emulsion.

[0174] Embodiment 15:

[0175] This embodiment provides a method for preparing an emulsion for improving the survival rate of hippocampus seedlings, comprising:

[0176] S100: washing and screening the perilla seeds, then subjecting the raw material of the perilla seeds to microwave drying at a temperature of 85° C., and then subjecting the raw material of the perilla seeds to wall breaking treatment using a wall breaking machine, and finally obtaining a perilla seed wall breaking material with a wall breaking particle size of 50 meshes;

[0177] S200: mixing the perilla seed wall-broken material and water in a mass ratio of 1:1, and then performing vacuum cooking treatment under sealed conditions, and controlling the cooking temperature to be 55° C., the cooking time to be 20 min, and the vacuum degree to be 0.05 MPa, to obtain a perilla seed mixture;

[0178] S300: performing supercritical extraction on the perilla seed mixture, controlling the extraction pressure of the supercritical extraction at 38 MPa, the extraction temperature at 40° C., the separation pressure at 9 MPa, the separation temperature at 50° C., and the extraction time at 6 h to obtain a perilla seed extract;

[0179] S400: maltodextrin, soy protein, seaweed polysaccharide and pure water in a mass ratio of 2.3:3.7:1:700 are mixed, and homogenized at a speed of 14000r / min for 75s to obtain a first mixture; then a perilla seed extract in a mass ratio of 3.5:5 is mixed with the first mixture, and homogenized at a speed of 15000r / min for 2.5min to obtain a second mixture; the second mixture is stirred while dripping 15wt% hydrochloric acid solution in water conditions of 50°C until the pH value of the second mixture reaches 4.5, and then spray-dried to obtain an embedded perilla seed extract; the extracted material in S300 is mixed with the first mixture, and the mixture is homogenized at a speed of 15000r / min for 2.5min to obtain a second mixture; The remaining residue is taken out, washed with pure water and air-dried at 85°C, and then ultrafinely crushed with a grinder to obtain a residue powder with a particle size of 150um-180um; the residue powder is then mixed with distilled water in a mass ratio of 10:200, and then homogenized at a speed of 13000r / min for 1min to obtain an auxiliary agent; the embedded perilla seed extract, the auxiliary agent and the β-cyclodextrin / gum arabic mixed solution in a mass ratio of 2:1:18 are mixed, and the speed of the emulsification homogenization treatment is controlled at 15000r / min and the treatment time is 5min, and finally a perilla seed oil Pickering emulsion is obtained.

[0180] Survival test of seahorse seedlings

[0181] (1) Breeding of seahorse fry: Mature, artificially bred second-generation American lineated seahorses with a body length of approximately 18-21 cm were selected and paired in a 1:1 ratio of male to female and then released into a 45 cm × 70 cm × 60 cm aquarium, with one pair placed in each aquarium; during the breeding period, the salinity, water temperature, light intensity and photoperiod were 30‰, 27±0.5℃, 1000lux and 14L:10D, respectively; when the American lineated seahorses hatched, the seahorse fry were immediately transferred to an aquarium with the same physical and chemical conditions.

[0182] (2) Breeding of Artemia nauplii: Artemia eggs soaked in fresh water for 60 min were placed in seawater with a salinity of 25‰ at a concentration of 3 g / L. The incubation temperature was controlled at 27±0.5℃, and the air was aerated. The illumination was 2000 lux. After 24 hours, Artemia nauplii were collected using phototaxis.

[0183] (3) Strengthening of Artemia nauplii: 30,000 cfu / L Artemia nauplii were stocked in 20 L water buckets, 0 ml / L of Perilla seed oil Pickering emulsion was added to the buckets of Group A, 0.15 ml / L of Perilla seed oil Pickering emulsion prepared in Example 1 was added to the buckets of Group B, 0.30 ml / L of Perilla seed oil Pickering emulsion prepared in Example 1 was added to the buckets of Group C, 0.15 ml / L of Perilla seed oil Pickering emulsion prepared in Example 11 was added to the buckets of Group D, and 0.30 ml / L of Perilla seed oil Pickering emulsion prepared in Example 11 was added to the buckets of Group E. To the barrels in group F, 0.15 ml / L of the Perilla seed oil Pickering emulsion prepared in Example 14 was added; to the barrels in group G, 0.30 ml / L of the Perilla seed oil Pickering emulsion prepared in Example 14 was added; to the barrels in group H, 0.15 ml / L of the Perilla seed oil Pickering emulsion prepared in Example 15 was added; to the barrels in group I, 0.30 ml / L of the Perilla seed oil Pickering emulsion prepared in Example 15 was added; each barrel was aerated for 12 hours; finally, each group of Artemia was taken out, filtered and rinsed using a 100-mesh silk screen.

[0184] (4) The 10-day-old American lineated seahorse seedlings used in the experiment were selected from two pairs of parent larvae, 20 of which were placed in each aquarium, and three replicates were set for each concentration. The fish were fed twice a day at 8:00 and 16:00, respectively, and the residual bait and feces were sucked out before each feeding. The experiment lasted for 30 days.

[0185] Experimental results: The survival rate of the hippocampus seedlings in group A was 11.2±1.74%, the survival rate of the hippocampus seedlings in group B was 32.4±3.7%, and the survival rate of the hippocampus seedlings in group C was 74.7±4.9%, and the survival rate of group C was significantly higher than that of group A and group B; the survival rate of the hippocampus seedlings in group D was 31.6±2.9%, and the survival rate of the hippocampus seedlings in group E was 74.3±4.6%, and the survival rate of group E was significantly higher than that of group A and group D. At the same time, by comparing the survival rates of group B and group D and group C and group E, it can be seen that the effects of the perilla seed oil Pickering emulsion prepared in Example 11 and Example 1 on the survival rate of the hippocampus seedlings are similar, that is, the embedding treatment of perilla seed oil in Example 11 does not have much effect on the use effect of the perilla seed oil Pickering emulsion. The survival rate of seahorse seedlings in group F was 33.5±3.9%, and the survival rate of seahorse seedlings in group G was 75.6±5.1%. The comparison between group B and group F, and between group C and group G showed that the addition of adjuvants could improve the survival rate of seahorse seedlings to a certain extent. The survival rate of seahorse seedlings in group H was 32.3±3.4%, and the survival rate of seahorse seedlings in group I was 74.8±4.7%. The comparison between group D and group H, and between group E and group I showed that the addition of adjuvants could improve the survival rate of seahorse seedlings to a certain extent.

[0186] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined by the claims.

Claims

1. A method for preparing an emulsion for improving the survival rate of hippocampus seedlings, characterized in that: include: S100: performing a wall-breaking treatment on a raw material of perilla seeds to obtain a wall-broken perilla seed material; S200: mixing the perilla seed wall-broken material with water, and then performing a vacuum cooking process to obtain a perilla seed mixed material; S300: performing supercritical extraction on the perilla seed mixture to obtain a perilla seed extract; S400: mixing the perilla seed extract with a β-cyclodextrin / gum arabic mixed solution, and then performing an emulsification and homogenization treatment to obtain a perilla seed oil Pickering emulsion.

2. The preparation method according to claim 1, characterized in that The S100 specifically includes the following steps: A perilla seed raw material is taken, the perilla seed raw material is first subjected to microwave drying, and then subjected to the wall breaking treatment to obtain the perilla seed wall-broken material with a wall-broken particle size of 40 meshes to 80 meshes.

3. The preparation method according to claim 1, characterized in that: The S200 specifically includes the following steps: The perilla seed wall-breaking material is mixed with water in a mass ratio of 1:(0.8-1.2), and then the vacuum cooking treatment is carried out under sealed conditions, and the cooking temperature is controlled at 50°C-60°C, the cooking time is controlled at 15min-25min, and the vacuum degree is controlled at 0.03MPa-0.07MPa to obtain the perilla seed mixture.

4. The preparation method according to claim 1, characterized in that: In the S300, the extraction pressure of the supercritical extraction is controlled at 35MPa-40MPa, the extraction temperature is controlled at 35°C-45°C, the separation pressure is controlled at 8MPa-10MPa, the separation temperature is controlled at 45°C-55°C, and the extraction time is controlled at 5h-7h.

5. The preparation method according to claim 4, characterized in that: In the S300, the yield of the perilla seed extract obtained after the supercritical extraction is controlled to be 32%-36%.

6. The preparation method according to claim 1, characterized in that: In the S400, the β-cyclodextrin / gum arabic mixed solution is prepared by the following method: S411: mixing β-cyclodextrin and distilled water in a mass ratio of (1-3):100, and then homogenizing at a speed of 8000 r / min-12000 r / min for 45 s-75 s, and then stirring with a magnetic stirrer until the β-cyclodextrin is completely dissolved in the distilled water to obtain a β-cyclodextrin solution; S412: mixing gum arabic and distilled water in a mass ratio of (1-3):100, and then homogenizing at a speed of 8000 r / min-12000 r / min for 45 s-75 s, and then stirring with a magnetic stirrer until the gum arabic is completely dissolved in the distilled water to obtain a gum arabic solution; S413: Mixing the β-cyclodextrin solution and the gum arabic solution in a mass ratio of 1:(0.8-1.2), and then concentrating to obtain the β-cyclodextrin / gum arabic mixed solution.

7. The preparation method according to claim 6, characterized in that: In the S413, the volume ratio of the mixed volume of the β-cyclodextrin solution and the gum arabic solution to the volume of the β-cyclodextrin / gum arabic mixed solution is 20:(17-19).

8. The preparation method according to claim 1, characterized in that: In the S400, the perilla seed extract is mixed with the β-cyclodextrin / gum arabic mixed solution in a mass ratio of (1.9-2.1):(17-19), and the rotation speed of the emulsification homogenization treatment is controlled at 12000r / min-18000r / min and the treatment time is controlled at 4min-6min, finally obtaining the perilla seed oil Pickering emulsion.

9. The preparation method according to any one of claims 1 to 8, characterized in that: In S400, after the perilla seed extract is subjected to embedding treatment, it is mixed with the β-cyclodextrin / gum arabic mixed solution, wherein the specific steps of the embedding treatment are as follows: S510: mixing maltodextrin, soy protein, seaweed polysaccharide, and pure water in a mass ratio of (2-3):(3-4):(0.9-0.11):(690-710) to obtain a first mixture; S520: mixing the perilla seed extract with the first mixed material in a mass ratio of (3-4):5 to obtain a second mixed material; S530: spray-drying the second mixed material to obtain the embedded perilla seed extract.

10. The preparation method according to claim 9, characterized in that: In the S510, maltodextrin, soy protein, seaweed polysaccharide and pure water are homogenized at a speed of 13000 r / min-15000 r / min for 60 s-90 s to obtain the first mixture; In the S520, the perilla seed extract and the first mixed material are homogenized at a speed of 14000 r / min-16000 r / min for 2 min-3 min to obtain the second mixed material in an emulsion state.