Repair method for treating periodontitis by using dental pulp stem cells

Abstract

The invention relates to the technical field of dental oral treatment, and discloses a repair method for treating periodontitis by using dental pulp stem cells, which comprises the following steps: S1, extracting dental pulp tissues from the oral cavity of a patient; s2, obtaining dental pulp stem cells; s3, culturing the dental pulp stem cells under specific conditions; s4, inducing the dental pulp stem cells to differentiate in the direction of osteogenesis, chondrogenesis or periodontal ligament cell formation; s5, transplanting the differentiated dental pulp stem cells to a periodontitis injury area of the patient, and using a biocompatible material; s6, applying a proper local stimulation means around the cell transplantation area; s7, by applying anti-inflammatory drugs locally or systemically, local immune response and inflammatory response are retarded, and repair and regeneration of periodontium are promoted. The problems that an existing treatment method is not lasting in effect, long in recovery period and the like are solved, the damaged periodontium is repaired through the dental pulp stem cells, the treatment effect can be remarkably improved, and the dental pulp stem cells have high clinical application value.

Description

Technical Field

[0001] The present invention relates to the technical field of dental oral treatment, and in particular to a repair method for treating periodontitis by using dental pulp stem cells. Background Art

[0002] Periodontitis is a common oral disease characterized by inflammation of the gums and surrounding supporting tissues (such as the periodontal ligament, alveolar bone, etc.), which can lead to loose teeth or tooth loss in severe cases. The treatments for periodontitis currently mainly include mechanical removal of periodontal calculus, scaling, periodontal surgery, and the use of antibiotics. However, these methods often cannot completely cure periodontitis, and have certain side effects and limitations, such as long recovery time and short-lasting treatment effects.

[0003] In recent years, dental pulp stem cells have received extensive attention in tissue repair and regenerative medicine due to their good proliferation ability and multidirectional differentiation potential. Dental pulp stem cells can differentiate into osteoblasts, chondrocytes and other types of cells through certain methods, and then be used to repair damaged periodontal tissues. However, the technology of using dental pulp stem cells to treat periodontitis has not yet been widely used, and its clinical treatment methods and specific operation steps are still imperfect, lacking effective and safe repair technology.

[0004] Therefore, we propose a restorative approach to treat periodontitis using dental pulp stem cells. Summary of the invention

[0005] The present invention mainly solves the technical problems existing in the above-mentioned prior art and provides a repair method for treating periodontitis using dental pulp stem cells.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme, a repair method for treating periodontitis using dental pulp stem cells, comprising the following steps:

[0007] S1: extracting dental pulp tissue from the patient's oral cavity, wherein the dental pulp tissue can be extracted from wisdom teeth, deciduous teeth or tooth root canals;

[0008] S2: Disinfect, separate and culture the extracted dental pulp tissue to obtain dental pulp stem cells;

[0009] S3: culturing the dental pulp stem cells under specific conditions, wherein the conditions include using a cell culture medium containing fetal bovine serum or other serum substitutes, and regulating cell growth factors such as insulin, transforming growth factor β (TGF-β) or bone morphogenetic protein (BMP-2) during the culture process to promote cell proliferation and differentiation;

[0010] S4: By applying a combination of multiple growth factors and cytokines, dental pulp stem cells are induced to differentiate into osteoblasts, cartilage-forming cells or periodontal ligament-forming cells to enhance the repair effect;

[0011] S5: Transplant the differentiated dental pulp stem cells into the patient's periodontitis damaged area and fix them with biocompatible materials such as gelatin sponge, hydroxyapatite, polylactic acid-glycolic acid copolymer, etc. to ensure the colonization and growth of cells in the damaged area;

[0012] S6: Apply appropriate local stimulation methods around the cell transplantation area, including low-frequency current, laser irradiation, ultrasound or microwave to accelerate cell proliferation and tissue repair;

[0013] S7: Through local or systemic application of anti-inflammatory drugs, local immune and inflammatory responses can be slowed down to promote the repair and regeneration of periodontal tissues.

[0014] As a preferred technical solution of the present invention, the dental pulp stem cells in S2 are separated by combining mechanical method with enzymatic method to improve separation purity and cell survival rate.

[0015] As a preferred technical solution of the present invention, the cell culture medium in S3 further contains vitamin C, L-glutamine and antibiotics to support the proliferation and long-term survival of dental pulp stem cells.

[0016] As a preferred technical solution of the present invention, the growth factors inducing differentiation of dental pulp stem cells in S4 include bone morphogenetic protein (BMP-2), transforming growth factor β (TGF-β), osteoinductive protein (OP-1) and fibronectin, and the concentration of the factors can be appropriately adjusted according to the needs of different patients.

[0017] As a preferred technical solution of the present invention, after the dental pulp stem cells are transplanted in S5, a local drug delivery system is further used to deliver growth factors, anti-inflammatory drugs and repair factors to the transplanted area in the form of nanoparticles or microspheres to improve the targeting and sustained release effect of the drugs.

[0018] As a preferred technical solution of the present invention, the biocompatible material in S5 is a natural polysaccharide material or a synthetic polymer material, which has excellent biodegradability and can be gradually degraded and absorbed by the body during the cell colonization process, avoiding the potential risks caused by material residues.

[0019] As a preferred technical solution of the present invention, after the dental pulp stem cells are transplanted, cytokine co-culture technology is further used to enhance the interaction between dental pulp stem cells and periodontal tissues, and promote directional differentiation of cells and tissue repair.

[0020] As a preferred technical solution of the present invention, the dental pulp stem cells are subjected to auxiliary treatment by low-frequency current or laser irradiation after transplantation to stimulate cell proliferation and differentiation and promote blood circulation in the repair area, thereby accelerating the regeneration of periodontal tissue.

[0021] As a preferred technical solution of the present invention, after the dental pulp stem cell transplantation, dynamic monitoring is performed by regularly taking X-rays or using 3D CT imaging technology to ensure the repair process of periodontal tissues and adjust the treatment plan in real time to improve the personalization and accuracy of the treatment.

[0022] The present invention provides a repair method for treating periodontitis using dental pulp stem cells. It has the following beneficial effects:

[0023] 1. This restorative method for treating periodontitis using dental pulp stem cells can effectively repair periodontal damage, overcome the problems of existing treatment methods such as short-term effects and long recovery periods, and solve the defects of traditional treatment methods. By using dental pulp stem cells to repair damaged periodontal tissues, the treatment effect can be significantly improved, and it has a high clinical application value.

[0024] 2. This method of restoring periodontitis using dental pulp stem cells uses the patient's own dental pulp stem cells, thus avoiding rejection and infectious problems and having the advantage of high safety.

[0025] 3. This is a restorative method for treating periodontitis using dental pulp stem cells. By combining the use of multiple growth factors (such as BMP-2, TGF-β, etc.) and cytokine co-culture technology, the differentiation potential of dental pulp stem cells is significantly enhanced, enabling them to more effectively differentiate into target cell types (such as osteoblasts, chondrocytes, etc.), thereby achieving the repair of periodontitis and the periodontal tissue damage caused by it. With proper cell culture, differentiation and stimulation, dental pulp stem cells can effectively promote the regeneration of periodontal tissues, improve periodontitis symptoms, enhance tooth stability, and have a significant repair effect.

[0026] 4. This restorative method of treating periodontitis using dental pulp stem cells can be used to treat mild, moderate and severe periodontitis, is suitable for patients of different degrees, and is widely used.

[0027] 5. This is a repair method for periodontitis using dental pulp stem cells. Through local stimulation and drug delivery system, physical stimulation methods such as low-frequency current and laser irradiation are combined with nano-drug delivery technology to enhance the treatment effect and repair efficiency. Through these auxiliary measures, cell proliferation, differentiation and repair processes can be accelerated, and the targeting and durability of treatment can be improved.

[0028] 6. This restorative method for treating periodontitis using dental pulp stem cells uses natural polysaccharide materials and degradable polymer materials as cell fixation materials, which not only ensures the successful cell colonization, but also avoids the risks brought by long-term residual biomaterials, further improving the safety and effectiveness of the treatment.

[0029] 7. This is a repair method for periodontitis using dental pulp stem cells. During the treatment process, modern imaging technologies such as X-rays and 3D CT imaging are used to achieve real-time dynamic monitoring of the periodontal tissue repair process. This personalized and precise treatment plan can be adjusted in a timely manner according to the patient's specific situation, improving the efficiency and success rate of treatment. DETAILED DESCRIPTION

[0030] Example 1

[0031] A restorative method for treating periodontitis using dental pulp stem cells comprises the following steps:

[0032] S1: Extract dental pulp tissue from the patient's mouth. The dental pulp tissue can be extracted from wisdom teeth, deciduous teeth or tooth root canals;

[0033] S2: Disinfect, separate and culture the extracted dental pulp tissue to obtain dental pulp stem cells;

[0034] S3: Dental pulp stem cells are cultured under specific conditions, including the use of a cell culture medium containing fetal bovine serum or other serum substitutes, and the regulation of cell growth factors such as insulin, transforming growth factor β (TGF-β) or bone morphogenetic protein (BMP-2) during the culture process to promote cell proliferation and differentiation;

[0035] S4: By applying a combination of multiple growth factors and cytokines, dental pulp stem cells are induced to differentiate into osteoblasts, cartilage-forming cells or periodontal ligament-forming cells to enhance the repair effect;

[0036] S5: Transplant the differentiated dental pulp stem cells into the patient's periodontitis damaged area and fix them with biocompatible materials such as gelatin sponge, hydroxyapatite, polylactic acid-glycolic acid copolymer, etc. to ensure the colonization and growth of cells in the damaged area;

[0037] S6: Apply appropriate local stimulation methods around the cell transplantation area, including low-frequency current, laser irradiation, ultrasound or microwave to accelerate cell proliferation and tissue repair;

[0038] S7: Through local or systemic application of anti-inflammatory drugs, local immune and inflammatory responses can be slowed down to promote the repair and regeneration of periodontal tissues.

[0039] Dental pulp stem cells were isolated by combining mechanical and enzymatic methods to improve separation purity and cell survival rate.

[0040] The cell culture medium further contains vitamin C, L-glutamine and antibiotics to support the proliferation and long-term survival of dental pulp stem cells.

[0041] Growth factors that induce differentiation of dental pulp stem cells include bone morphogenetic protein (BMP-2), transforming growth factor β (TGF-β), osteoinductive protein (OP-1) and fibronectin, and the concentration of the factors can be appropriately adjusted according to the needs of different patients.

[0042] After dental pulp stem cell transplantation, a local drug delivery system is further used to deliver growth factors, anti-inflammatory drugs and repair factors to the transplanted area in the form of nanoparticles or microspheres to improve the targeting and sustained release of drugs.

[0043] Biocompatible materials are natural polysaccharide materials or synthetic polymer materials, which have excellent biodegradability and can be gradually degraded and absorbed by the body during the cell colonization process, avoiding the potential risks caused by material residues.

[0044] After dental pulp stem cell transplantation, cytokine co-culture technology is further used to enhance the interaction between dental pulp stem cells and periodontal tissues, promote cell directional differentiation and tissue repair.

[0045] After transplantation, dental pulp stem cells are treated with low-frequency current or laser irradiation to stimulate cell proliferation and differentiation and promote blood circulation in the repair area, thereby accelerating the regeneration of periodontal tissue.

[0046] After dental pulp stem cell transplantation, dynamic monitoring is performed by regularly taking X-rays or using 3D CT imaging technology to ensure the repair process of periodontal tissues and adjust the treatment plan in real time to improve the personalization and accuracy of treatment.

[0047] Through the extraction and use of autologous dental pulp stem cells, the patient's own dental pulp stem cells are used as a repair carrier for the treatment of periodontitis. This not only avoids the problem of immune rejection of allogeneic cells, but also provides a more biocompatible treatment plan, significantly improving the safety and effectiveness of the treatment.

[0048] Based on cell differentiation and multi-factor induction, the differentiation potential of dental pulp stem cells is significantly improved by combining the use of multiple growth factors (such as BMP-2, TGF-β, etc.) and cytokine co-culture technology, enabling them to differentiate more effectively into target cell types (such as osteoblasts, chondrocytes, etc.), thereby achieving the repair of periodontitis and the periodontal tissue damage caused by it.

[0049] Through local stimulation and drug delivery systems, physical stimulation methods such as low-frequency current and laser irradiation are combined with nano-drug delivery technology to enhance the therapeutic effect and repair efficiency. Through these auxiliary measures, cell proliferation, differentiation and repair processes can be accelerated, and the targeting and durability of treatment can be improved.

[0050] The use of natural polysaccharide materials and degradable polymer materials as cell fixation materials not only ensures the successful cell colonization, but also avoids the risks brought by long-term biomaterial residues, further improving the safety and effectiveness of treatment.

[0051] During the treatment process, modern imaging technologies such as X-rays and 3D CT imaging are used to achieve real-time dynamic monitoring of the periodontal tissue repair process. This personalized and precise treatment plan can be adjusted in a timely manner according to the patient's specific situation, improving the efficiency and success rate of treatment.

[0052] Example 2

[0053] On the basis of Example 1, the patient of this example is a 40-year-old male with mild periodontitis. First, dental pulp tissue was extracted from his wisdom tooth. After the extracted dental pulp tissue was processed, it was cultured with DMEM / F12 culture medium containing 10% fetal bovine serum to obtain dental pulp stem cells. Then, BMP-2 growth factor was added during the culture process to promote osteogenic differentiation of the cells. The differentiated cells were transplanted into the periodontitis lesion area, and gelatin sponge was used as a biocompatible material to fix the cells. After the operation, the patient took oral anti-inflammatory drugs and used low-frequency current stimulation to promote cell proliferation. Three months later, X-ray examination found that the periodontal tissue had recovered, the patient's teeth were stable, and the inflammation was significantly relieved.

[0054] Example 3

[0055] On the basis of Example 1, the patient of this example is a 60-year-old female with moderate periodontitis and loose teeth. During the treatment, dental pulp tissue is extracted from deciduous teeth, dental pulp stem cells are isolated and obtained, and their differentiation into chondrocytes is promoted by adding TGF-β growth factor. The differentiated cells are transplanted into the patient's periodontal lesion area by local injection and fixed with natural polysaccharide-based materials. After the operation, the patient is stimulated to proliferate cells by local laser irradiation. After two months of treatment, the patient's tooth mobility was reduced, periodontal inflammation was significantly reduced, and the patient reported that the pain was significantly reduced.

[0056] Example 4

[0057] On the basis of Example 1, the patient of this example is a 35-year-old male who has suffered from severe periodontitis for a long time and has severe alveolar bone absorption. In this case, dental pulp tissue is extracted from wisdom teeth, and dental pulp stem cells are separated and cultured. In order to enhance the effect of cell differentiation, BMP-2 growth factor is used. After the transplanted dental pulp stem cells are successfully implanted in the periodontal damage area, the patient is treated with local anti-inflammatory drugs and undergoes regular X-ray examinations to confirm that alveolar bone absorption is effectively controlled, gingival tissue is repaired, and the patient's symptoms are improved.

[0058] Example 5

[0059] On the basis of Example 1, the patient of this example is a 50-year-old woman who suffers from periodontitis with alveolar bone loss. The doctor extracted pulp tissue from her deciduous teeth, separated dental pulp stem cells and cultured them in vitro. In order to promote the osteogenic differentiation of dental pulp stem cells, BMP-2 growth factor was added, and biodegradable materials were used to fix the cells. During the treatment, the patient underwent local low-frequency current stimulation. Six months after the operation, through imaging examination and clinical examination, the repair effect of periodontal tissue was significant, and the alveolar bone showed obvious signs of regeneration.

[0060] Working principle of the present invention:

[0061] The repair method of the present invention repairs the damaged site of periodontitis by utilizing the patient's own dental pulp stem cells. Dental pulp stem cells have a strong differentiation potential. Under appropriate conditions, they can differentiate into osteoblasts, chondrocytes, etc. to repair damaged periodontal tissues. By adding appropriate growth factors (such as BMP-2, TGF-β, etc.) during in vitro culture, the differentiation of dental pulp stem cells into target cell types can be promoted to enhance the repair effect. After cell transplantation, biocompatible materials are used to fix the cells to ensure their colonization and growth at the site of periodontal damage, and local stimulation (such as laser irradiation, electric current stimulation, etc.) is used to further promote cell proliferation and differentiation. Ultimately, the periodontal tissue is effectively repaired and tooth stability is improved.

[0062] The above method can effectively repair periodontal damage, overcome the problems of short-lasting effects and long recovery periods of existing treatment methods, and solve the defects of traditional treatment methods. By using dental pulp stem cells to repair damaged periodontal tissues, the treatment effect can be significantly improved, which has a high clinical application value.

[0063] High safety: The present invention uses the patient's own dental pulp stem cells, thus avoiding rejection and infectious problems.

[0064] Significant repair effect: Through proper cell culture, differentiation and stimulation, dental pulp stem cells can effectively promote the regeneration of periodontal tissue, improve periodontitis symptoms and enhance tooth stability.

[0065] Wide application: This method can be used to treat mild, moderate and severe periodontitis and is suitable for patients of different degrees.

[0066] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A repair method for treating periodontitis using dental pulp stem cells, characterized in that: The following steps are involved: S1: extracting dental pulp tissue from the patient's oral cavity, wherein the dental pulp tissue can be extracted from wisdom teeth, deciduous teeth or tooth root canals; S2: Disinfect, separate and culture the extracted dental pulp tissue to obtain dental pulp stem cells; S3: culturing the dental pulp stem cells under specific conditions, wherein the conditions include using a cell culture medium containing fetal bovine serum or other serum substitutes, and regulating cell growth factors such as insulin, transforming growth factor β (TGF-β) or bone morphogenetic protein (BMP-2) during the culture process to promote cell proliferation and differentiation; S4: By applying a combination of multiple growth factors and cytokines, dental pulp stem cells are induced to differentiate into osteoblasts, cartilage-forming cells or periodontal ligament-forming cells to enhance the repair effect; S5: Transplant the differentiated dental pulp stem cells into the patient's periodontitis damaged area and fix them with biocompatible materials such as gelatin sponge, hydroxyapatite, polylactic acid-glycolic acid copolymer, etc. to ensure the colonization and growth of cells in the damaged area; S6: Apply appropriate local stimulation methods around the cell transplantation area, including low-frequency current, laser irradiation, ultrasound or microwave to accelerate cell proliferation and tissue repair; S7: Through local or systemic application of anti-inflammatory drugs, local immune and inflammatory responses can be slowed down to promote the repair and regeneration of periodontal tissues.

2. A repair method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: The dental pulp stem cells in S2 are separated by combining mechanical method with enzymatic method to improve separation purity and cell survival rate.

3. The repair method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: The cell culture medium in S3 further comprises vitamin C, L-glutamine and antibiotics to support the proliferation and long-term survival of dental pulp stem cells.

4. The method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: The growth factors inducing differentiation of dental pulp stem cells in S4 include bone morphogenetic protein (BMP-2), transforming growth factor β (TGF-β), osteoinductive protein (OP-1) and fibronectin, and the concentration of the factors can be appropriately adjusted according to the needs of different patients.

5. The method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: After the dental pulp stem cells are transplanted in S5, a local drug delivery system is further used to deliver growth factors, anti-inflammatory drugs and repair factors to the transplanted area in the form of nanoparticles or microspheres to improve the targeting and sustained release effect of the drugs.

6. The method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: The biocompatible material in S5 is a natural polysaccharide material or a synthetic polymer material, which has excellent biodegradability and can be gradually degraded and absorbed by the body during the cell colonization process, thus avoiding the potential risks caused by material residues.

7. The repair method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: After the dental pulp stem cells are transplanted, cytokine co-culture technology is further used to enhance the interaction between the dental pulp stem cells and the periodontal tissue, and promote the directional differentiation of cells and tissue repair.

8. The method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: After transplantation, the dental pulp stem cells are subjected to auxiliary treatment by low-frequency current or laser irradiation to stimulate cell proliferation and differentiation and promote blood circulation in the repair area, thereby accelerating the regeneration of periodontal tissue.

9. The repair method for treating periodontitis using dental pulp stem cells according to claim 1, characterized in that: After the dental pulp stem cell transplantation, dynamic monitoring is performed by regularly taking X-rays or using 3D CT imaging technology to ensure the repair process of the periodontal tissue and adjust the treatment plan in real time to improve the personalization and accuracy of the treatment.