Sample gene detection workstation
By designing an automatic sample transfer and ventilation system in the DNA detection workstation, the pollution problem during sample transfer is solved, the detection accuracy and efficiency are improved, the labor intensity is reduced, and more efficient sample processing is achieved.
Patent Information
- Application Number
- CN202310832071.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-07
- Publication Date
- 2025-01-07
AI Technical Summary
Existing DNA testing workstations are prone to sample contamination during sample delivery, affecting the accuracy of detection results, and are highly labor-intensive and inefficient.
A sample gene detection workstation was designed, including a rack, a detection operation bin and a sample transfer bin. The transfer mechanism and sample processing unit were used to automatically transmit samples between different operation bins, combined with a ventilation system to prevent external pollution, and through positive pressure and negative Pressure control environment to reduce labor intensity for personnel.
It improves the accuracy and efficiency of the test results, avoids sample contamination, reduces the labor intensity of the testers, and improves the safety of the work.
Smart Images

Figure CN119265004A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a sample gene detection workstation, and in particular to a sample gene detection workstation capable of improving the accuracy and efficiency of detection results. Background Art
[0002] During the DNA testing process, the DNA testing workstation usually needs to transfer samples between multiple testing rooms, and the corresponding testing rooms complete the corresponding testing steps, and finally complete all the testing operation steps. In the prior art, when transferring samples between multiple testing rooms, the testing operator usually directly opens the door of a testing room, manually takes the sample from the testing room, and then opens the door of another testing room, thereby manually placing the sample in the set position in the testing room. This method will cause the sample to be directly exposed to the outside world, causing contamination to the sample, thereby affecting the accuracy of the test results, and resulting in high labor intensity for the testing operators, and most importantly, it will lead to low work efficiency. Summary of the invention
[0003] In view of the above-mentioned shortcomings of the prior art, the technical problem to be solved by the present invention is to provide a sample gene detection workstation that can improve the detection efficiency, reduce labor intensity, and improve the accuracy of the detection results.
[0004] To achieve the above-mentioned objectives, the present invention provides a sample gene testing workstation, comprising a frame, a first testing operation chamber installed on the frame, a second testing operation chamber installed on the frame, and a sample transfer chamber, wherein the first testing operation chamber is provided with a first sample processing unit and a first transfer mechanism, and the second testing operation chamber is provided with a second sample processing unit and a second transfer mechanism, the sample transfer chamber comprises a transfer chamber body, and a transfer platform arranged in the transfer chamber body, a first window is provided at one end of the transfer chamber body, a first switch door is provided at the first window, a second window is provided at the other end of the transfer chamber body, a second switch door is provided at the second window, when the first switch door is opened, the inner cavity of the transfer chamber body is communicated with the inner cavity of the first testing operation chamber through the first window, and when the second switch door is opened, the inner cavity of the transfer chamber body is communicated with the inner cavity of the second testing operation chamber through the second window.
[0005] Furthermore, the sample gene testing workstation also includes a ventilation system, which includes a first ventilation unit connected to the first testing operation chamber, and a second ventilation unit connected to the second testing operation chamber, the first ventilation unit is used to form a positive pressure in the first testing operation chamber, and the second ventilation unit is used to form a negative pressure in the second testing operation chamber.
[0006] Further, the first ventilation unit includes a first air inlet duct connected to the first inspection operation chamber, and a first air outlet duct connected to the first inspection operation chamber, and the first air inlet duct includes an air supply fan; the second ventilation unit includes a second air inlet duct connected to the second inspection operation chamber, and a second air outlet duct connected to the second inspection operation chamber, and the second air outlet duct includes a suction fan.
[0007] Furthermore, a transfer drive motor, a transfer driving wheel installed on the output end of the transfer drive motor, and a transfer driven wheel connected to the transfer driving wheel through a transfer transmission belt are installed in the transfer bin body, and the transfer platform is connected to the transfer transmission belt.
[0008] Further, the first sample processing unit includes a purification mechanism, the purification mechanism includes a magnetic sleeve plate grabbing module, the magnetic sleeve plate grabbing module includes a grabbing main board frame, a rotating shaft installed on the side of the grabbing main board frame, a swing claw arranged on the rotating shaft, and a rotating shaft driving assembly connected to the rotating shaft, and the rotating shaft driving assembly can drive the rotating shaft to rotate.
[0009] Furthermore, the shaft driving assembly includes a transmission swing arm, a transmission joint and a gripper lifting frame that can be raised and lowered relative to the grab main frame, the end of the shaft is inserted into the shaft hole of the grab main frame, one end of the transmission swing arm is connected to the end of the shaft, the other end of the transmission swing arm is connected to one end of the transmission joint, and the other end of the transmission joint is embedded in the transmission groove of the gripper lifting frame.
[0010] Furthermore, a grabbing drive motor is provided on the gripper lifting frame, and the grabbing drive motor is sleeved on the purification screw rod, and the purification screw rod is also sleeved with a magnetic rod lifting drive motor, and the magnetic rod lifting drive motor is connected to the magnetic rod lifting frame, and a magnetic rod is installed on the magnetic rod lifting frame.
[0011] Furthermore, the first sample processing unit also includes a first pipetting mechanism, a film sticking mechanism, a heating device, a centrifuge, a first film pressing mechanism, and a first manipulator, and the first film pressing mechanism and the purification mechanism are both installed on a first longitudinal motion frame of the longitudinal translation drive module, the first pipetting mechanism is installed on the transverse translation drive module, and the transverse translation drive module is installed on the second longitudinal motion frame of the longitudinal translation drive module.
[0012] Furthermore, the second sample processing unit includes a second pipetting mechanism, a second press-fit film mechanism, an amplification instrument and a second manipulator, the second pipetting mechanism and the second press-fit film mechanism are both installed on the first linear module, and the first linear module is installed on the second linear module, the second linear module can drive the first linear module to move in the front-to-back direction, and the first linear module can drive the second pipetting mechanism and the second press-fit film mechanism to move in the left-to-right direction.
[0013] Furthermore, the side wall of the first detection operation chamber includes a first observation chamber wall, and the side wall of the second detection operation chamber includes a second observation chamber wall, and the first observation chamber wall and the second observation chamber wall are both made of transparent material.
[0014] As described above, the sample gene detection workstation according to the present invention has the following beneficial effects:
[0015] The working principle of the sample gene detection workstation is as follows: in the first detection operation chamber, the first transfer mechanism is used to transfer the sample and the like accordingly, and the first sample processing unit is used to perform corresponding processing procedures on the sample, such as pipetting, heating, centrifugation, purification, pressing film, etc. on the sample, and then the first switch door of the transfer chamber is opened, and the first transfer mechanism is used to transfer the processed sample to the transfer table in the transfer chamber through the first window, the first switch door is closed, and the transfer table drives the sample to move from the first window to the second window, the second switch door is opened, and the second transfer mechanism takes out the sample on the transfer table through the second window and transfers it to the set position in the second detection operation chamber, and the second switch door is opened. Close, and then combine with the second sample processing unit to continue to process the sample accordingly, such as amplifying, pipetting, pressing the film, etc. This operation method can set the processing procedures with different requirements for the operating environment in different operation chambers to complete, meet more sample processing needs, and improve the final detection accuracy; and based on the above-mentioned sample transfer chamber, while realizing the transfer of samples from the first detection operation chamber to the second detection operation chamber, it can avoid direct exposure of the sample to the outside world, thereby avoiding contamination of the sample by the external environment, thereby improving the detection accuracy, and this method of transferring samples is more efficient than the method of directly taking and transferring samples manually, and reduces the labor intensity of detection personnel. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 This is a first-perspective stereoscopic image of a sample gene detection workstation in an embodiment of the present invention.
[0017] Figure 2 This is a second perspective stereoscopic image of the sample gene detection workstation in an embodiment of the present invention.
[0018] Figure 3 Schematic diagram of the internal structure of the sample gene detection workstation in an embodiment of the present invention.
[0019] Figure 4 Schematic diagram of the structure of the purification mechanism in the embodiment of the present invention.
[0020] Figure 5 It is a schematic diagram of the structure when the purification mechanism grabs the magnetic sleeve plate in an embodiment of the present invention.
[0021] Figure 6Schematic diagram of the relative positions of the sample transfer chamber, the first detection operation chamber and the second detection operation chamber in an embodiment of the present invention.
[0022] Figure 7 Schematic diagram of the structure of the sample transfer chamber in an embodiment of the present invention.
[0023] Figure 8 Schematic diagram of the internal structure of the sample transfer chamber in an embodiment of the present invention.
[0024] Component number description
[0025] 1 Frame 25 First observation chamber wall
[0026] 2 The first inspection warehouse 3 The second inspection warehouse
[0027] 21 Purification Agency 31 Amplification Instrument
[0028] 211 Grab the main frame 32 Second manipulator
[0029] 212 Rotating shaft 33 First linear module
[0030] 213 swing claw 34 second linear module
[0031] 214 Transmission swing arm 35 Second observation chamber wall
[0032] 215 Transmission joint 4 Sample transfer chamber
[0033] 216 gripper lifting frame 41 transfer bin
[0034] 217 Grab drive motor 42 Transfer table
[0035] 218 Magnetic bar lifting drive motor 43 First window
[0036] 219 Magnetic bar lifting frame 44 First opening and closing door
[0037] 22 First pipetting mechanism 45 Second window
[0038] 23 Heating device 46 Second switch door
[0039] 24 First Robot DETAILED DESCRIPTION
[0040] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.
[0041] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change in the proportion relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the effects and purposes that can be achieved by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. The change or adjustment of their relative relationship should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.
[0042] like Figures 1 to 8As shown, this embodiment provides a sample gene detection workstation, including a rack 1, a first detection operation chamber 2 installed on the rack 1, a second detection operation chamber 3 installed on the rack 1, and a sample transfer chamber 4, the first detection operation chamber 2 is provided with a first sample processing unit and a first transfer mechanism, the second detection operation chamber 3 is provided with a second sample processing unit and a second transfer mechanism, the sample transfer chamber 4 includes a transfer chamber body 41, and a transfer platform 42 arranged in the transfer chamber body 41, one end of the transfer chamber body 41 is provided with a first window 43, the first window 43 is provided with a first switch door 44, the other end of the transfer chamber body 41 is provided with a second window 45, the second window 45 is provided with a second switch door 46, when the first switch door 44 is opened, the inner cavity of the transfer chamber body 41 is communicated with the inner cavity of the first detection operation chamber 2 through the first window 43, and when the second switch door 46 is opened, the inner cavity of the transfer chamber body 41 is communicated with the inner cavity of the second detection operation chamber 3 through the second window 45. The working principle of the sample gene detection workstation is as follows: in the first detection operation chamber 2, the first transfer mechanism is used to transfer the sample and the like accordingly, and the first sample processing unit is combined to perform corresponding processing procedures on the sample, such as pipetting, heating, centrifugation, purification, pressing film, etc., and then the first switch door 44 of the transfer chamber body 41 is opened, and the first transfer mechanism is used to transfer the processed sample to the transfer table 42 in the transfer chamber body 41 through the first window 43, and the first switch door 44 is closed, and the transfer table 42 drives the sample to move from the first window 43 to the second window 45, and the second switch door 46 is opened, and the second transfer mechanism takes out the sample on the transfer table 42 through the second window 45 and transfers it to the second detection operation chamber 3 In the set position, the second switch door 46 is closed, and then the second sample processing unit is combined to continue to process the sample accordingly, such as amplifying, pipetting, pressing the film, etc. In this operation mode, the processing procedures with different requirements for the operating environment can be set in different operation chambers to complete, so as to meet more sample processing needs and improve the final detection accuracy; and based on the above-mentioned sample transfer chamber 4, while realizing the transfer of the sample from the first detection operation chamber 2 to the second detection operation chamber 3, it can avoid the sample from being directly exposed to the outside world, thereby avoiding the contamination of the sample by the external environment, and improving the detection accuracy. Moreover, compared with the method of directly taking and transferring the sample manually, this method of transferring the sample is more efficient and reduces the labor intensity of the detection personnel.
[0043] In this embodiment, the sample gene detection workstation also includes a ventilation system, which includes a first ventilation unit connected to the first detection operation warehouse 2 and a second ventilation unit connected to the second detection operation warehouse 3. When the sample gene detection workstation is running, the air in the first detection operation warehouse 2 is ventilated based on the first ventilation unit, and the air in the second detection operation warehouse 3 is ventilated based on the second ventilation unit, so as to ensure that the air index in the first detection operation warehouse 2 and the air index in the second detection operation warehouse 3 meet the set requirements respectively. The most important thing is that based on the ventilation effect of the first ventilation unit, a positive pressure will be formed in the first detection operation warehouse 2, and the positive pressure can effectively prevent the outside air from entering the first detection operation warehouse 2 through the gap or when the warehouse door is opened to load materials, thereby affecting the air index in the first detection operation warehouse 2, and then affecting the corresponding processing procedures in the first detection operation warehouse 2. Due to the characteristics of the corresponding processing procedures in the second detection operation warehouse 3, the air in the second detection operation warehouse 3 is often toxic. If the air in the second detection operation warehouse 3 flows out to the on-site environment outside the warehouse when the warehouse door is opened, it will cause harm to the personal health of the detection personnel. To this end, this embodiment forms a negative pressure in the second inspection operation chamber 3 based on the ventilation effect of the second ventilation unit. The negative pressure prevents the air in the second inspection operation chamber 3 from flowing out to the outside through the gap or when the chamber door is opened for loading, thereby improving the safety of the operation.
[0044] At the same time, in this embodiment, the first ventilation unit includes a first air inlet duct connected to the first detection operation warehouse 2, and a first air outlet duct connected to the first detection operation warehouse 2, and the first air inlet duct includes a blower; the second ventilation unit includes a second air inlet duct connected to the second detection operation warehouse 3, and a second air outlet duct connected to the second detection operation warehouse 3, and the second air outlet duct includes a suction fan. The air in the external set area enters the first detection operation warehouse 2 through the first air inlet duct, and the air in the first detection operation warehouse 2 is discharged to the set area through the first air outlet duct. The outside air enters the second detection operation room through the second air inlet duct, and the air in the second detection operation room is discharged to the set area through the second air outlet duct. The first air inlet duct can actively supply air to the first detection operation warehouse 2 under the action of the blower, ensuring that the air pressure in the first detection operation warehouse 2 is greater than the external air pressure, so that the air pressure in the first detection operation warehouse 2 is positive pressure compared with the external air pressure. Under the action of the exhaust fan, the second air outlet can actively extract the air in the second detection operation chamber 3 to the set area, so that the air pressure in the second detection operation chamber 3 is lower than the external air pressure. In this way, the air pressure in the second detection operation chamber 3 is negative pressure compared with the external air pressure.
[0045] like Figure 7 and Figure 8As shown, in this embodiment, a transfer drive motor, a transfer driving wheel installed on the output end of the transfer drive motor, and a transfer driven wheel connected to the transfer driving wheel through a transfer transmission belt are installed in the transfer warehouse body 41, and the transfer platform 42 is connected to the transfer transmission belt. In this way, when the transfer drive motor rotates forward, it drives the transfer driving wheel and the transfer transmission belt to rotate forward, and the transfer transmission belt drives the transfer platform 42 to move from the first window 43 to the second window 45. When the transfer drive motor is reversed, it drives the transfer driving wheel and the transfer transmission belt to reverse, and the transfer transmission belt drives the transfer platform 42 to move from the second window 45 to the first window 43. At the same time, in this embodiment, a transfer guide rail is provided in the transfer warehouse body 41, and the bottom of the transfer platform 42 is slidably matched with the transfer guide rail, and the transfer guide rail supports and guides the transfer platform 42. In addition, in the present embodiment, two window switch drive motors and two sets of screw transmission mechanisms are also installed on the inner wall of the transfer bin body 41. The two window switch drive motors are respectively connected to the two sets of screw transmission mechanisms, and the two sets of screw transmission mechanisms are respectively connected to the first switch door 44 and the second switch door 46. The two window switch drive motors drive the first switch door 44 and the second switch door 46 to open or close through the two sets of screw transmission mechanisms.
[0046] like Figure 1 , Figure 4 and Figure 5 As shown, the first sample processing unit includes a purification mechanism 21, the purification mechanism 21 includes a magnetic sleeve grab module, the magnetic sleeve grab module includes a grab main frame 211, a rotating shaft 212 installed on the side of the grab main frame 211, a swing claw 213 set on the rotating shaft 212, and a rotating shaft driving component connected to the rotating shaft 212, and the rotating shaft driving component can drive the rotating shaft 212 to rotate. The first sample processing unit performs corresponding purification operations based on the purification mechanism 21. In the present embodiment, when the purification mechanism 21 is in the process of grabbing the magnetic sleeve, when the magnetic sleeve grabbing module corresponds to the position of the magnetic sleeve, the shaft driving assembly is used to drive the shaft 212 to rotate. When the shaft 212 rotates, it will drive the swing claw 213 to swing until the swing claw 213 is tightly against the lower surface of the magnetic sleeve, and the magnetic sleeve is pressed against the lower surface of the grabbing main frame 211. Based on the abutment relationship between the grabbing main frame 211 and the swing claw 213 and the upper and lower surfaces of the magnetic sleeve, the magnetic sleeve is grabbed. In this way, the magnetic sleeve can be grabbed more smoothly and firmly, and damage to the magnetic sleeve can be avoided. At the same time, if Figure 5As shown, the shaft driving assembly in this embodiment includes a transmission swing arm 214, a transmission joint 215, and a gripper lifting frame 216 that can be lifted and lowered relative to the grab main frame 211. The end of the shaft 212 is inserted into the shaft hole of the grab main frame 211. One end of the transmission swing arm 214 is connected to the end of the shaft 212, and the other end of the transmission swing arm 214 is connected to one end of the transmission joint 215. The other end of the transmission joint 215 is embedded in the transmission groove of the gripper lifting frame 216. When the grabbing action needs to be performed, the gripper lifting frame 216 descends, and through the matching relationship between the transmission groove and the transmission joint 215, the other end of the transmission swing arm 214 is driven to swing downward. When the transmission swing arm 214 swings, it drives the shaft 212 to rotate, thereby realizing the swing claw 213 swinging and grasping the magnetic sleeve plate. In this embodiment, both sides of the grab main frame 211 are equipped with rotating shafts 212, and each rotating shaft 212 is provided with two swing claws 213. Both sides of the grab lifting frame 216 are provided with transmission grooves, and the ends of the two rotating shafts 212 are respectively connected to two transmission swing arms 214, and the two transmission swing arms 214 are respectively connected to one end of two transmission joints 215, and the other ends of the two transmission joints 215 are respectively embedded in the two transmission grooves of the grab lifting frame 216. In this way, when the grab lifting frame 216 is lifted or lowered, it will drive the two rotating shafts 212 to rotate respectively, and then drive the swing claws 213 on the two rotating shafts 212 to swing, so as to grasp or release the magnetic sleeve. Two positioning ear grooves are provided on both sides of the magnetic sleeve. When one rotating shaft 212 rotates, it drives the two swing claws 213 thereon to be respectively embedded in the two positioning ear grooves located on the same side. When the other rotating shaft 212 rotates, it drives the two swing claws 213 thereon to be respectively embedded in the other two positioning ear grooves located on the same side, so as to capture the magnetic sleeve and ensure that the relative position of the magnetic sleeve and the capture main frame 211 in the front-back, left-right and up-down directions accurately meets the set requirements.
[0047] like Figure 4As shown, in this embodiment, a grabbing drive motor 217 is provided on the gripper lifting frame 216, and the grabbing drive motor 217 is sleeved on the purification screw rod, and the purification screw rod is also sleeved with a magnetic rod lifting drive motor 218, and the magnetic rod lifting drive motor 218 is connected to the magnetic rod lifting frame 219, and a magnetic rod is installed on the magnetic rod lifting frame 219. When the grabbing drive motor 217 is running, it will drive the gripper lifting frame 216 to rise and fall along the purification screw rod to grasp the magnetic sleeve plate. When the magnetic rod lifting drive motor 218 is running, it will drive the magnetic rod lifting frame 219 and the magnetic rod to rise and fall, so that the magnetic rod is inserted downward into the magnetic sleeve of the magnetic sleeve plate, thereby completing the insertion of the magnetic rod into the grasped magnetic sleeve plate. Moreover, the magnetic rod and the grabbing and lifting frame are both lifted and lowered along the purification screw rod, ensuring that the relative position of the magnetic rod and the grabbing and lifting frame in the horizontal direction is accurately within the set requirements. At the same time, the grabbing and lifting frame is connected to the rotating shaft 212 and the grabbing main frame 211 based on the above structure, thereby ensuring that the relative position of the magnetic rod and the grasped magnetic sleeve plate in the horizontal direction maintains the set requirements during the lifting process, and then the magnetic rod can be accurately inserted into the magnetic sleeve when it moves downward to the set position.
[0048] like Figure 1 and Figure 3As shown, the first sample processing unit in this embodiment also includes a first pipetting mechanism 22, a film-sticking mechanism, a heating device 23, a centrifuge, a first film-pressing mechanism, and a first manipulator 24. The first pipetting mechanism 22 is used to perform corresponding pipetting actions on corresponding reagents and samples in the first detection operation chamber 2, such as sucking a sample from one consumable and then injecting the sample into another consumable; the film-sticking mechanism can stick a film on a deep-well plate containing corresponding samples; the heating device 23 can heat the deep-well plate after sticking the film; the centrifuge can centrifuge the heated deep-well plate and the matched plate obtained by pipetting; the first film-pressing mechanism can press the rubber on the well plate containing the corresponding sample, which well plate can be a PCR plate; the first manipulator 24 is used to transfer various consumables in the first detection operation chamber 2 accordingly, and cooperate with the above-mentioned actuators to complete detection and processing procedures such as pipetting, film-sticking, heating, centrifugation and PCR plate construction. The first press-fit film mechanism and the purification mechanism 21 are both installed on the first longitudinal motion frame of the longitudinal translation drive module, the first liquid transfer mechanism 22 is installed on the transverse translation drive module, and the transverse translation drive module is installed on the second longitudinal motion frame of the longitudinal translation drive module. In the process of press-fitting the rubber on the orifice plate, the first longitudinal motion frame can be used to drive the first press-fit film mechanism to move longitudinally to meet the corresponding movement requirements of the first press-fit film mechanism; when performing the purification operation, the first longitudinal motion frame can also be used to drive the purification mechanism 21 to move longitudinally to complete the corresponding action. When performing liquid transfer, the second longitudinal motion frame of the longitudinal translation drive module can be used to drive the transverse translation drive module and the first liquid transfer mechanism 22 to move longitudinally to meet the longitudinal movement requirements of the first liquid transfer mechanism 22, and the transverse translation drive module can be used to drive the first liquid transfer mechanism 22 to move transversely to meet the transverse movement requirements of the first liquid transfer mechanism 22. In this embodiment, the first sample processing unit adopts the above-mentioned structural design, which simplifies its overall structure while meeting the requirements of performing multiple treatments on the sample, so that it occupies less space, is convenient for layout, and improves the operation efficiency.
[0049] like Figure 1 and Figure 3As shown, in this embodiment, the second sample processing unit includes a second pipetting mechanism, a second press-fitting film mechanism, an amplifier 31 and a second manipulator 32. The amplifier 31 can be used to amplify the PCR plate transferred from the first detection operation chamber 2 to the second detection operation chamber 3. The second pipetting mechanism can transfer the amplified product to the new PCR plate and the corresponding reagent to the new PCR plate; the second press-fitting film mechanism can press-fit the electrophoresis rubber onto the new PCR plate. The second pipetting mechanism and the second press-fitting film mechanism are both installed on the first linear module 33, and the first linear module 33 is installed on the second linear module 34. The second linear module 34 can drive the first linear module 33 to move in the front-to-back direction, and the first linear module 33 can drive the second pipetting mechanism and the second press-fitting film mechanism to move in the left-to-right direction. In this way, the first linear module 33 and the second linear module 34 are used to realize the translation function of the second pipetting mechanism and the second press-fitting film mechanism in the front-to-back and left-to-right directions, and the second sample processing unit structure design is more compact and smaller in size.
[0050] like Figures 1 to 3 As shown, the side wall of the first detection operation chamber 2 includes a first observation chamber wall 25, and the side wall of the second detection operation chamber 3 includes a second observation chamber wall 35. The first observation chamber wall 25 and the second observation chamber wall 35 are both made of transparent materials, and specifically, the first observation chamber wall 25 and the second observation chamber wall 35 can be made of glass. When the sample gene detection workstation performs the corresponding detection process, the staff can observe the conditions in the first detection operation chamber 2 and the second detection operation chamber 3 through the first observation chamber wall 25 and the second observation chamber wall 35, respectively, so that the staff can intuitively understand the operation of the sample gene detection workstation.
[0051] In this embodiment, the sample transfer bin 4 also includes an air suction component connected to one end of the transfer bin body 41, and a fresh air supply component connected to the other end of the transfer bin body 41. During the sample transfer process, the air suction component is used to suck away the gas in the transfer bin body 41, and the fresh air supply component is used to supply new air to the transfer bin body 41, so as to avoid the gas flowing into the transfer bin body 41 from one detection operation bin when the window is open, and flowing into another detection operation bin when the other window is open, thereby polluting the environment of the other detection operation bin, thereby improving the detection accuracy. The air suction component includes an air suction filter, and the fresh air supply component includes a fresh air filter.
[0052] In this embodiment, the sample gene detection workstation is specifically a DNA detection workstation. In this embodiment, the second sample processing unit also includes a gene electrophoresis instrument, which can be used to perform gene sequencing detection on the corresponding sample to complete the corresponding DNA detection. In this embodiment, the first sample processing unit is specifically used to realize the construction of the PCR plate. After obtaining the PCR plate, the first manipulator 24 is used to transfer the obtained PCR plate to the transfer platform 42 of the sample transfer warehouse 4 through the first window 43, and the transfer platform 42 transfers the PCR plate to the second window 45. The second switch door 46 is opened, and the second manipulator 32 transfers the PCR plate to the set position in the second detection operation warehouse 3 through the second window 45, and then the second sample processing unit is used to perform corresponding amplification, pipetting, press-fitting electrophoresis rubber, constructing electrophoresis kit, and gene sequencing and other processing procedures, and finally complete the DNA detection.
[0053] In summary, the present invention effectively overcomes various shortcomings of the prior art and has high industrial utilization value.
[0054] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.
Claims
1. A sample gene detection workstation, characterized in that: The invention comprises a frame (1), a first detection operation chamber (2) mounted on the frame (1), a second detection operation chamber (3) mounted on the frame (1), and a sample transfer chamber (4), wherein the first detection operation chamber (2) is provided with a first sample processing unit and a first transfer mechanism, the second detection operation chamber (3) is provided with a second sample processing unit and a second transfer mechanism, the sample transfer chamber (4) comprises a transfer chamber body (41), and a transfer platform (42) arranged in the transfer chamber body (41), and one end of the transfer chamber body (41) is provided with a first window (43), a first opening and closing door (44) is provided at the first window (43), a second window (45) is provided at the other end of the transfer bin body (41), and a second opening and closing door (46) is provided at the second window (45). When the first opening and closing door (44) is opened, the inner cavity of the transfer bin body (41) communicates with the inner cavity of the first detection operation bin (2) through the first window (43); when the second opening and closing door (46) is opened, the inner cavity of the transfer bin body (41) communicates with the inner cavity of the second detection operation bin (3) through the second window (45).
2. The sample gene detection workstation according to claim 1, characterized in that: The invention also comprises a ventilation system, wherein the ventilation system comprises a first ventilation unit connected to the first detection operation chamber (2) and a second ventilation unit connected to the second detection operation chamber (3), wherein the first ventilation unit is used to form a positive pressure in the first detection operation chamber (2), and the second ventilation unit is used to form a negative pressure in the second detection operation chamber (3).
3. The sample gene detection workstation according to claim 2, characterized in that: The first ventilation unit comprises a first air inlet duct connected to the first inspection operation chamber (2) and a first air outlet duct connected to the first inspection operation chamber (2), the first air inlet duct comprising an air supply fan; the second ventilation unit comprises a second air inlet duct connected to the second inspection operation chamber (3) and a second air outlet duct connected to the second inspection operation chamber (3), the second air outlet duct comprising a suction fan.
4. The sample gene detection workstation according to claim 1, characterized in that: The transfer bin body (41) is equipped with a transfer drive motor, a transfer driving wheel installed on the output end of the transfer drive motor, and a transfer driven wheel connected to the transfer driving wheel via a transfer transmission belt, and the transfer platform (42) is connected to the transfer transmission belt.
5. The sample gene detection workstation according to claim 1, characterized in that: The first sample processing unit comprises a purification mechanism (21), the purification mechanism (21) comprises a magnetic sleeve grabbing module, the magnetic sleeve grabbing module comprises a grabbing main frame (211), a rotating shaft (212) installed on the side of the grabbing main frame (211), a swing claw (213) arranged on the rotating shaft (212), and a rotating shaft driving component connected to the rotating shaft (212), wherein the rotating shaft driving component can drive the rotating shaft (212) to rotate.
6. The sample gene detection workstation according to claim 5, characterized in that: The rotating shaft driving assembly comprises a transmission swing arm (214), a transmission joint (215) and a gripper lifting frame (216) capable of being lifted and lowered relative to a grabbing main frame (211); an end of the rotating shaft (212) is inserted into a rotating shaft hole of the grabbing main frame (211); one end of the transmission swing arm (214) is connected to the end of the rotating shaft (212); the other end of the transmission swing arm (214) is connected to one end of the transmission joint (215); and the other end of the transmission joint (215) is embedded in a transmission groove of the gripper lifting frame (216).
7. The sample gene detection workstation according to claim 6, characterized in that: The gripper lifting frame (216) is provided with a grabbing drive motor (217), the grabbing drive motor (217) is sleeved on the purification screw rod, and the purification screw rod is also sleeved with a magnetic bar lifting drive motor (218), and the magnetic bar lifting drive motor (218) is connected to the magnetic bar lifting frame (219), and the magnetic bar lifting frame (219) is equipped with a magnetic bar.
8. The sample gene detection workstation according to claim 5, characterized in that: The first sample processing unit also includes a first pipetting mechanism (22), a film sticking mechanism, a heating device (23), a centrifuge, a first film pressing mechanism, and a first manipulator (24), and the first film pressing mechanism and the purification mechanism (21) are both installed on a first longitudinal motion frame of the longitudinal translation drive module, the first pipetting mechanism (22) is installed on a transverse translation drive module, and the transverse translation drive module is installed on a second longitudinal motion frame of the longitudinal translation drive module.
9. The sample gene detection workstation according to claim 1, characterized in that: The second sample processing unit comprises a second pipetting mechanism, a second press-fitting film mechanism, an amplification instrument (31) and a second manipulator (32); the second pipetting mechanism and the second press-fitting film mechanism are both mounted on a first linear module (33), and the first linear module (33) is mounted on a second linear module (34); the second linear module (34) can drive the first linear module (33) to move in a front-to-back direction, and the first linear module (33) can drive the second pipetting mechanism and the second press-fitting film mechanism to move in a left-to-right direction.
10. The sample gene detection workstation according to claim 1, characterized in that: The side wall of the first detection operation chamber (2) comprises a first observation chamber wall (25), and the side wall of the second detection operation chamber (3) comprises a second observation chamber wall (35), and both the first observation chamber wall (25) and the second observation chamber wall (35) are made of transparent material.