Beverage containing agilawood leaf Pu'er tea extract and preparation method thereof

Abstract

The invention discloses a beverage containing agilawood leaf Pu'er tea extract and a preparation method of the beverage, and particularly belongs to the field of functional foods and beverages. The beverage comprises a compound composition and a food or medicine acceptable solvent, wherein the compound composition comprises the following components: an agilawood leaf Pu'er tea extract, a red yeast rice extract and a brown rice extract. According to the beverage provided by the invention, through scientific proportioning, the synergistic effects of the agilawood leaf Pu'er tea extract, the red yeast rice extract and the brown rice extract in the aspects of improving metabolism, promoting lipolysis, protecting liver health and the like are exerted; the effects of reducing the blood fat level, promoting liver cell fat metabolism, reducing fat accumulation and improving the obese fatty liver are achieved.

Description

Technical Field

[0001] The invention belongs to the field of functional food and beverage, and particularly relates to a beverage containing agarwood leaf and Pu'er tea extract and a preparation method thereof. Background Art

[0002] With the acceleration of modern life and changes in dietary structure, obesity has become an increasingly serious health challenge worldwide. Obesity not only affects an individual's appearance and self-confidence, but more importantly, it is often accompanied by a variety of metabolic diseases, among which fatty liver disease is particularly prominent. Fatty liver disease is caused by excessive accumulation of fat in the body, especially excessive accumulation of triglycerides in the liver, which not only leads to impaired liver function, but may also further develop into cirrhosis and even liver cancer, seriously threatening human life and health.

[0003] In the process of searching for effective methods to prevent and treat fatty liver caused by obesity, researchers have found that natural plant extracts have attracted much attention due to their safety and health characteristics. Agarwood leaves, as the leaves of the Thymelaeaceae plant Aquilaria sinensis, have gradually become a research hotspot in recent years due to their rich bioactive ingredients and multiple pharmacological effects. Agarwood leaves are rich in calcium, zinc, iron, manganese, vitamins and other essential elements for the human body, as well as polysaccharides, amino acids, flavonoids, glycosides and phenols. These ingredients have multiple biological activities such as antioxidant, anti-tumor, anti-inflammatory and analgesic, and play an important role in improving metabolic diseases and protecting liver health.

[0004] Pu'er tea, as a traditional Chinese famous tea, also has multiple health functions. Studies have shown that tea polyphenols, catechins and other ingredients in Pu'er tea help lower blood lipids, regulate blood sugar, promote fat metabolism, and have a certain preventive and therapeutic effect on obesity and related diseases such as fatty liver.

[0005] However, natural extracts of a single ingredient often fail to achieve the desired preventive and therapeutic effects. Beverages have gradually become a new research direction due to their advantages of combining multiple ingredients. Through scientific and reasonable compounding, not only can the synergistic effect between the ingredients be enhanced to improve the preventive and therapeutic effects, but also the taste and flavor of the beverage can be improved, increasing consumer acceptance. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a beverage containing agarwood leaf and Pu'er tea extract and a preparation method thereof. The beverage is scientifically compounded with agarwood leaf and Pu'er tea extract, red yeast rice extract and brown rice extract, aiming to exert the synergistic effects of multiple ingredients in improving metabolism, promoting fat decomposition, protecting liver health, etc., thereby achieving the purpose of preventing and treating obese fatty liver.

[0007] To achieve the above object, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a composite composition containing an extract of agarwood leaves and Pu'er tea, wherein the composition comprises the following components in parts by mass:

[0009] 1-1.5 parts of agarwood leaf puer tea extract;

[0010] 0.7-1 part of red yeast rice extract;

[0011] Brown rice extract 0.9-1.3 parts.

[0012] Preferably, the agarwood leaf and Pu'er tea extract is obtained by mixing agarwood leaves and Pu'er tea in a mass ratio of (14-16):10 and extracting using an ethanol aqueous solution.

[0013] Further preferably, the method for preparing the agarwood leaf Pu'er tea extract comprises the following steps:

[0014] S1-1 weighed dry agarwood leaves and Pu'er tea according to mass ratio were crushed and sieved through a 60-mesh sieve to obtain a mixed material;

[0015] S1-2. Mix and soak the mixed material with a 75 v / v% ethanol aqueous solution which is 3 times the weight of the mixed material, and reflux extract at 60°C for 2 times, each time for 30 min. Combine the extracts, filter and collect the filtrate, and concentrate the filtrate under reduced pressure and evaporate to dryness to obtain the agarwood leaf Pu'er tea extract.

[0016] Preferably, the preparation method of the red yeast rice extract comprises the following steps: adding deionized water to the red yeast rice at a solid-liquid ratio of 2:1 g / mL, and extracting at 95°C for 30 minutes, filtering and collecting the filtrate, and concentrating the filtrate under reduced pressure and evaporating to obtain the red yeast rice extract.

[0017] Preferably, the method for preparing the brown rice extract comprises the following steps:

[0018] S2-1. The brown rice was crushed through a 100-mesh sieve to obtain brown rice flour;

[0019] S2-2. Add deionized water to brown rice flour with a solid-liquid ratio of 1:2 g / mL and add enzyme activity of (2-3)×10 5 U / g of cellulase, the addition amount is 4-6wt% of the brown rice flour, stirring at 50r / min for 1h, high-speed centrifugation, filtering, and obtaining a filter residue and a supernatant A for standby use;

[0020] S2-3. Add 95 v / v% ethanol aqueous solution to the filter residue obtained in step S2-2, the solid-liquid ratio of the filter residue to the ethanol aqueous solution is 1:3 g / mL, reflux extraction is performed at 60°C for 3 times, each time for 50 min, the extracts are combined, the filtrate B is filtered and collected, the supernatant A and the filtrate B are combined, and homogenized and dispersed at 3000 r / min for 10 min to obtain a mixed solution AB, the mixed solution AB is concentrated under reduced pressure and evaporated to obtain a brown rice extract.

[0021] In a second aspect, the present invention provides a beverage containing an extract of agarwood leaves and Pu'er tea, wherein the beverage comprises the compound composition described in the first aspect and a solvent acceptable to food or medicine.

[0022] Preferably, the compound composition is added to the beverage in an amount of 2.6-3.8 wt %.

[0023] More preferably, the beverage also includes a thickener, an emulsifier, and a flavoring agent.

[0024] More preferably, the thickener is β-cyclodextrin;

[0025] The emulsifier is modified soybean lecithin;

[0026] The flavoring agent is at least one of fructooligosaccharide, galacto-oligosaccharide and erythritol.

[0027] In a third aspect, the present invention provides a method for preparing the beverage according to the second aspect, the preparation method comprising the following steps:

[0028] S3-1. Accurately weigh each raw material according to the dosage ratio and set aside;

[0029] S3-2. The agarwood leaf Pu'er tea extract, red yeast rice extract and brown rice extract were added to the solvent and stirred until dissolved, and then oligofructose, galacto-oligosaccharide, erythritol, modified soybean lecithin, β-cyclodextrin were added successively, and stirred at 1000r / min to obtain a mixed solution;

[0030] S3-3. The mixed solution obtained in step S3-2 is canned and sterilized for 30 minutes to obtain a beverage containing agarwood leaf Pu'er tea extract.

[0031] In the present invention:

[0032] Agarwood leaf extract contains a lot of active ingredients such as polysaccharides, amino acids, flavonoids, benzophenone glycosides, terpenoids, and a small amount of volatile oils, which have pharmacological activities such as anti-oxidation, anti-inflammatory, hypoglycemic and lipid-regulating. Pu'er tea contains a variety of beneficial ingredients, including organic acids, various enzymes, polysaccharides, amino acids, tea polyphenols, catechins, and rich vitamins and minerals. These substances help digestion and enhance appetite, promote the absorption of nutrients, regulate the immune system, enhance physical strength and improve cardiovascular function, have antioxidant, anti-inflammatory and antibacterial effects, and help prevent cardiovascular and cerebrovascular diseases and cancer.

[0033] Red yeast rice extract contains a variety of ingredients, mainly including monacolin, sterols, red yeast pigments and other active ingredients. It has biological activities such as lipid regulation, anti-tumor, antioxidant, and anti-diabetic. Compared with refined lovastatin, the monacolin in red yeast rice extract has higher bioavailability and more effective cholesterol-lowering effect. It also reduces the accumulation of fat in liver cells and has the effect of anti-obesity and treatment of fatty liver.

[0034] Brown rice extract contains rich functional ingredients, such as γ-aminobutyric acid (GABA), oryzanol, inositol, glutathione, octacosanol, rice bran polysaccharides, etc., which have multiple physiological effects such as lowering blood pressure, anti-oxidation, anti-aging, and lowering blood lipids.

[0035] The present invention study found that the agarwood leaf Pu'er tea extract, red yeast rice extract and brown rice extract have a certain improvement effect on obesity-induced liver damage when used in combination. The agarwood leaf Pu'er tea extract plays a role by regulating blood lipids and reducing fat accumulation, the red yeast rice extract plays a role by lowering cholesterol and reducing fat accumulation in liver cells, and the brown rice extract plays a role by increasing the level of superoxide dismutase and reducing the percentage of fatty liver cells. The combined effect of these extracts helps to improve the symptoms of obese fatty liver.

[0036] Beneficial effects of the present invention:

[0037] 1. The composition of the agarwood leaf Pu'er tea extract, red yeast rice extract and brown rice extract provided by the present invention is a key functional ingredient in the whole drink. The composition provided by the present invention can significantly reduce the levels of serum liver function indicators ALT, AST and blood lipid indicators TC, TG and LDL-C in obese model rats, and the composition has a certain improvement effect on obese fatty liver;

[0038] 2. The composition provided by the present invention significantly inhibited and slowed down the body weight of obese model rats, proving that the composition has the efficacy of promoting liver cell metabolism of fat, reducing fat accumulation, and preventing and treating obesity. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. However, the following embodiments are only preferred embodiments of the present invention, not all. For the sake of clarity, all features of the actual embodiments are not described.

[0040] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without making any creative work shall all fall within the protection scope of the present invention.

[0041] In the present invention:

[0042] Aquilaria leaf: Aquilaria sinensis (Lour.) Spreng. Leaf, Thymelaeaceae, purchased from Zhonghe Medicinal Materials Wholesale;

[0043] Pu'er tea: Camellia sinensis var. Assamica (JW Mast.) Kitam., belonging to the Theaceae family, purchased from Yunnan Tongjinchang Tea Co., Ltd.;

[0044] Red yeast rice: Made from indica rice, japonica rice, glutinous rice and other rice, fermented with Monascus purpurogenum, it is brown-red or purple-red rice grains and was purchased from Wuhan Jiacheng Biological Products Co., Ltd.

[0045] Brown rice: whole-grain rice obtained by husking rice without or with minimal processing, consisting of three major parts: rice bran, embryo, and endosperm, purchased from Yunnan Yishanxiang Trading Co., Ltd.;

[0046] Other raw materials are commercially available.

[0047] Preparation of Aquilaria Leaf Pu'er Tea Extract:

[0048] S1-1 according to the mass ratio in Table 1 weighed dried agarwood leaves and Pu'er tea, and placed in a universal high-speed grinder for crushing, through a 60 mesh sieve to obtain a mixed material;

[0049] S1-2. The mixture was mixed and soaked with a 75v / v% ethanol aqueous solution 3 times the weight of the mixture, and extracted twice at 60°C under reflux, each time for 30min, the extracts were combined, filtered and the filtrate was collected, the filtrate was concentrated under reduced pressure and evaporated to dryness to obtain agarwood leaf Pu'er tea extract ①-③;

[0050] Agarwood leaf extract ④: Agarwood leaf is used alone as a raw material for extraction, and the extraction method is consistent with the above method to obtain agarwood leaf extract ④;

[0051] Pu'er tea extract ⑤: Pu'er tea is used alone as a raw material for extraction, and the extraction method is consistent with the above method to obtain Pu'er tea extract ⑤.

[0052] Table 1 Quality ratio of agarwood Pu'er

[0053]

[0054] Preparation of Red Yeast Rice Extract:

[0055] Deionized water was added to red yeast rice at a solid-liquid ratio of 2:1 (g / mL), and the mixture was extracted at 95°C for 30 minutes. The filtrate was filtered and collected, and the filtrate was concentrated and evaporated under reduced pressure to obtain a red yeast rice extract.

[0056] Preparation of Brown Rice Extract:

[0057] S2-1. After the brown rice was crushed using a universal high-speed mill, it was sieved through a 100-mesh sieve to obtain brown rice flour;

[0058] S2-2. Add deionized water to brown rice flour with a solid-liquid ratio of 1:2 g / mL and add enzyme activity of 3×10 5 U / g of cellulase, added in an amount of 5wt% of the brown rice flour, stirred at 50r / min for 1h, centrifuged at high speed, filtered, and obtained a filter residue and supernatant A for standby use;

[0059] S2-3. Add 95 v / v% ethanol aqueous solution to the filter residue obtained in step S2-2, the solid-liquid ratio of the filter residue to the ethanol aqueous solution is 1:3 g / mL, reflux extraction is performed at 60°C for 3 times, each time for 50 min, the extracts are combined, the filtrate B is filtered and collected, the supernatant A and the filtrate B are combined, and homogenized and dispersed at 3000 r / min for 10 min to obtain a mixed solution AB, the mixed solution AB is concentrated under reduced pressure and evaporated to obtain a brown rice extract.

[0060] Preparation of Example:

[0061] S3-1. Accurately weigh each raw material according to the dosage ratio in Table 2 and set aside;

[0062] S3-2. Add drinking water to the constant volume tank, the drinking water complies with the "GB 5749-2022 Drinking Water Hygiene Standard", the agarwood leaf Pu'er tea extract, red yeast rice extract and brown rice extract weighed in step S3-1 are added to the constant volume tank, stirred until dissolved, and then oligofructose, oligogalactose, erythritol, modified soybean lecithin, β-cyclodextrin are added in sequence, and stirred and mixed at 1000r / min to obtain a mixed solution;

[0063] S3-3. The mixed solution obtained in step S3-2 is canned and sterilized for 30 minutes to obtain a beverage containing agarwood leaf Pu'er tea extract.

[0064] Table 2 Mass percentage of raw materials prepared in Example

[0065]

[0066] Preparation of Comparative Example:

[0067] Except for the different raw material addition, the preparation steps are consistent with those in the embodiment. The specific raw material addition amounts are shown in Table 3;

[0068] Table 3 Mass percentage of raw materials prepared in comparative example

[0069]

[0070] Note: “-” in the table means not added.

[0071] Performance Testing:

[0072] Animal testing

[0073] 1. Experimental modeling and methods

[0074] 1.1 Test materials

[0075] 96 clean-grade healthy male SD rats, weighing (200±10) g, were purchased from Liaoning Changsheng Biotechnology Co., Ltd. All rats were raised in a clean and sterile environment. During the adaptive feeding period, the rats were housed in separate cages with a 12h:2h day and night cycle and free access to food.

[0076] Ordinary feed and D12451 high-fat feed were purchased from Beijing Ke'ao Xieli Feed Co., Ltd.

[0077] Silybin capsules (each capsule contains 35 mg of silybin, produced by Tianjin Tasly Saint Pharmaceutical Co., Ltd.); total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) kits were purchased from Coab Biotech.

[0078] Take the beverages of Example 3 and Comparative Examples 1-9 respectively, 250 mL of beverage per bottle, and set aside.

[0079] 1.2 Model establishment

[0080] 88 rats were randomly selected from 96 SD rats to establish obesity model, and the remaining 8 rats were set up as control group. Obesity rat model was established by high-fat diet induction method, 8 control group rats were fed with ordinary feed, and 88 obese model rats were fed with D12451 high-fat feed for 10 consecutive weeks and observed. The model was successful when the body weight exceeded the body weight of the control group rats by more than 20% during the same period, and the serum triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels increased, and the high-density lipoprotein cholesterol (HDL-C) level decreased.

[0081] 1.3 Trial Grouping and Dosing Method

[0082] 88 rats with successful obesity modeling were selected and randomly divided into comparative example group 9, silybin group, example group 3, and comparative example groups 1-8, with 8 rats in each group.

[0083] Comparative Example 9 group: The beverage corresponding to Comparative Example 9 was gavaged once a day at a rate of 0.0083 mL / (g·bw), which is equivalent to a 60 kg adult's daily intake of 500 mL of the beverage.

[0084] Silybin group: Silybin capsule powder was added to the drink prepared in Comparative Example 9 to dissolve into a gavage preparation, and 1.66 mL was gavaged once a day at a dose of 0.007 mg / (g·bw) of silybin, equivalent to a daily intake of 420 mg of silybin for an adult with a body weight of 60 kg.

[0085] Group 3 of Example 3: The beverage corresponding to Example 3 was gavaged once a day at a rate of 0.0083 mL / (g·bw), which is equivalent to a 60 kg adult's daily intake of 500 mL of the beverage.

[0086] Comparative Example 1-8 Group: The drinks corresponding to Comparative Examples 1-8 were gavaged once a day at 0.0083 mL / (g·bw), which is equivalent to a 60 kg adult's daily intake of 500 mL of the drink.

[0087] Control group: The beverage corresponding to Comparative Example 9 was gavaged once a day at 0.0083 mL / (g·bw), which is equivalent to a 60 kg adult's daily intake of 500 mL of the beverage.

[0088] The mice were gavaged continuously for 8 weeks. During the administration period, the modeling group continued to be fed with high-fat diet, and the control group was fed with ordinary diet. The mice were measured with a weighing machine (accurate to 0.01g) every week.

[0089] 2. Detection indicators

[0090] After 8 weeks of administration, 12 groups of rats were anesthetized intraperitoneally with 5 mL of 10% chloral hydrate, and blood samples were collected from the rats. The samples were centrifuged at 4500 r / min for 4 min, and the supernatant was extracted. The levels of blood lipids and liver function indicators TG, TC, LDL-C, HDL-C, ALT, and AST were detected using an automatic biochemical analyzer.

[0091] 3. Statistical methods

[0092] SPSS17.0 software was used for statistical analysis. Variance analysis was used for comparison of means between groups, and LSD method was used for multiple comparisons between groups; P < 0.05 was considered statistically significant.

[0093] 4. Test results

[0094] 4.1 Comparison of body weight among groups during medication

[0095] The changes in body weight of each group after 8 weeks of administration: the body weight of the modeling group was significantly higher than that of the control group (P < 0.01); at week 8, the body weight of Example 3 group was significantly lower than that of Comparative Example 9 group (P < 0.01), and the body weight of Example 3 group was significantly lower than that of Comparative Example 1-8 groups (P < 0.05). There was no significant difference between the silybin group and Comparative Example 9, as shown in Table 4.

[0096] Table 4 Comparison of body weight of rats in each group ( g)

[0097]

[0098]

[0099] Note: Time in the table indicates the number of weeks of administration; * indicates that the modeling group is compared with the control group, P < 0.05, ** indicates that the difference is extremely significant; a indicates that the Example 3 group is compared with the Comparative Example 9 group, P < 0.05, aa indicates that the difference is extremely significant; b indicates that the Comparative Examples 1-8 groups are compared with the Example 3 group, P < 0.05, bb indicates that the difference is extremely significant; c indicates that the Example 3 group is compared with the Silybin group, P < 0.05, cc indicates that the difference is extremely significant.

[0100] 4.2 Comparison of blood lipid indexes in each group during medication

[0101] Comparison of blood lipid function indicators in each group: TC, TG, and LDL-C levels in the modeling group were significantly higher than those in the control group, and the HDL-C level was significantly lower than that in the control group (P < 0.05); TC, TG, and LDL-C levels in the silybin group and Example 3 group were significantly lower than those in the comparative example 9 group, and the HDL-C level was significantly higher than that in the comparative example 9 group (P < 0.01); TC, TG, and LDL-C levels in the comparative example 1-8 groups were significantly higher than those in the exemplary embodiment 3 group, and the HDL-C level was significantly lower than that in the exemplary embodiment 3 group (P < 0.05), as shown in Table 5.

[0102] Table 5 Comparison of blood lipid function indicators in each group

[0103]

[0104] Note: In the table, a means that compared with the comparative example 9 group, P < 0.05, aa means that the difference is extremely significant; b means that the comparative examples 1-8 groups are compared with the example 3 group, P < 0.05, bb means that the difference is extremely significant; c means that the example 3 group is compared with the silybin group, P < 0.05, cc means that the difference is extremely significant.

[0105] 4.3 Comparison of liver function indicators in each group during drug administration

[0106] Comparison of liver function indicators in each group: ALT and AST levels in the modeling group were significantly higher than those in the control group (P < 0.05); ALT and AST levels in the silybin group and Example 3 group were significantly lower than those in the comparative example 9 group (P < 0.01); ALT and AST levels in the comparative example 1-8 groups were significantly higher than those in the exemplary example 3 group (P < 0.01), see Table 6.

[0107] Table 6 Comparison of liver function indicators in each group

[0108]

[0109] Note: In the table, a means that compared with the comparative example 9 group, P < 0.05, aa means that the difference is extremely significant; b means that the comparative examples 1-8 groups are compared with the example 3 group, P < 0.05, bb means that the difference is extremely significant; c means that the example 3 group is compared with the silybin group, P < 0.05, cc means that the difference is extremely significant.

[0110] 4.4 Conclusion Analysis

[0111] According to the results, the composition composed of the agarwood leaf Pu'er tea extract, red yeast rice extract and brown rice extract provided by the present invention is a key functional ingredient in the whole drink. According to the results in Tables 5 and 6, during the administration period, the serum liver function indexes ALT, AST and blood lipid indexes TC, TG and LDL-C levels of the rats in Example 3 group were significantly lower than those in the comparative example 9 group without key ingredients, which proves that the composition has a certain improvement effect on obese fatty liver; at the same time, according to the results in Table 4, the body weight gain of the rats in Example 3 in the 8th week was significantly lower than that in the comparative example 9 group, the silybin group and the comparative example 1-8 group, which proves that the composition has a certain inhibitory and slowing effect on the body weight growth of the obese rat model during the administration period, indicating that the composition has the effect of promoting liver cell metabolism of fat and reducing fat accumulation.

[0112] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composite composition containing an extract of agarwood leaves and Pu'er tea, characterized in that: The composition comprises the following components by weight: 1-1.5 parts of agarwood leaf puer tea extract; 0.7-1 part of red yeast rice extract; Brown rice extract 0.9-1.3 parts.

2. The composite composition according to claim 1, characterized in that The agarwood leaf and Pu'er tea extract is obtained by mixing agarwood leaves and Pu'er tea in a mass ratio of (14-16):10 and extracting with an ethanol aqueous solution.

3. The composite composition according to claim 2, characterized in that The preparation method of the agarwood leaf Pu'er tea extract comprises the following steps: S1-1 weighed dry agarwood leaves and Pu'er tea according to mass ratio were crushed and sieved through a 60-mesh sieve to obtain a mixed material; S1-2. Mix and soak the mixed material with a 75 v / v% ethanol aqueous solution which is 3 times the weight of the mixed material, and reflux extract at 60°C for 2 times, each time for 30 min. Combine the extracts, filter and collect the filtrate, and concentrate the filtrate under reduced pressure and evaporate to dryness to obtain the agarwood leaf Pu'er tea extract.

4. The composite composition according to claim 1, characterized in that The preparation method of the red yeast rice extract comprises the following steps: adding deionized water to the red yeast rice at a solid-liquid ratio of 2:1 g / mL, extracting at 95° C. for 30 minutes, filtering and collecting the filtrate, and concentrating and evaporating the filtrate under reduced pressure to obtain the red yeast rice extract.

5. The composite composition according to claim 1, characterized in that The preparation method of the brown rice extract comprises the following steps: S2-1. The brown rice was crushed through a 100-mesh sieve to obtain brown rice flour; S2-2. Add deionized water to brown rice flour with a solid-liquid ratio of 1:2 g / mL and add enzyme activity of (2-3)×10 5 U / g of cellulase, the addition amount is 4-6wt% of the brown rice flour, stirring at 50r / min for 1h, high-speed centrifugation, filtering, and obtaining a filter residue and a supernatant A for standby use; S2-3. Add 95 v / v% ethanol aqueous solution to the filter residue obtained in step S2-2, the solid-liquid ratio of the filter residue to the ethanol aqueous solution is 1:3 g / mL, reflux extraction is performed at 60°C for 3 times, each time for 50 min, the extracts are combined, the filtrate B is filtered and collected, the supernatant A and the filtrate B are combined, and homogenized and dispersed at 3000 r / min for 10 min to obtain a mixed solution AB, the mixed solution AB is concentrated under reduced pressure and evaporated to obtain a brown rice extract.

6. A beverage containing agarwood leaf Pu'er tea extract, characterized in that: The drink comprises the compound composition according to any one of claims 1 to 5, and a solvent acceptable to food or medicine.

7. The beverage according to claim 6, characterized in that The compound composition is added to the beverage in an amount of 2.6-3.8 wt %.

8. The beverage according to claim 7, characterized in that The beverage also includes a thickener, an emulsifier and a flavoring agent.

9. The beverage according to claim 8, characterized in that The thickener is β-cyclodextrin; The emulsifier is modified soybean lecithin; The flavoring agent is at least one of fructooligosaccharide, galacto-oligosaccharide and erythritol.

10. The method for preparing a beverage according to claim 9, characterized in that: The preparation method comprises the following steps: S3-1. Accurately weigh each raw material according to the dosage ratio and set aside; S3-2. The agarwood leaf Pu'er tea extract, red yeast rice extract and brown rice extract were added to the solvent and stirred until dissolved, and then oligofructose, galacto-oligosaccharide, erythritol, modified soybean lecithin, β-cyclodextrin were added successively, and stirred at 1000r / min to obtain a mixed solution; S3-3. The mixed solution obtained in step S3-2 is canned and sterilized for 30 minutes to obtain a beverage containing agarwood leaf Pu'er tea extract.

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