Preparation method and application of cynanchum auriculatum composition with lactagogue effect

Abstract

The invention provides a preparation method and application of a cynanchum auriculatum composition with a lactagogue effect, raw material powder is obtained after cynanchum auriculatum, colla corii asini, longan, fructus lycii, pawpaw, radix puerariae, rhizoma dioscoreae and poria cocos are pretreated, then the raw materials are extracted by adopting an ultrasonic-assisted subcritical water technology, and the cynanchum auriculatum composition is obtained. The preparation method is easy to operate, low in energy consumption, safe, environmentally friendly, high in extraction efficiency of plant raw materials and very suitable for low-cost large-scale production, and an oral preparation prepared from the cynanchum auriculatum composition is good in lactagogue effect and high in stability.

Description

Technical Field

[0001] The invention relates to the technical field of composition preparation, and in particular to a preparation method and application of an auricularia auricula composition with a lactation promoting effect. Background Art

[0002] Traditional Chinese medicine believes that "milk is made of qi and blood". Insufficient milk after childbirth is often related to qi and blood deficiency. Taking qi and blood-replenishing foods or medicines can achieve the effect of replenishing qi and blood and improving milk secretion. Ear leaf cow skin Xiao, also known as white shouwu, contains a variety of active ingredients such as polysaccharides and steroid compounds. It has the effect of nourishing the liver and kidneys, and benefiting the essence and blood, and can be used for lactation. Patent CN104998120A discloses a Chinese medicine formula for promoting postpartum recovery and lactation in pregnant women, which is prepared with Chinese medicines such as red medicine, white grass root, yarrow, motherwort, frankincense, Guangdong Liujinu, salvia miltiorrhiza, dragon tooth, cubeb, fennel, orange seed, white shouwu, dendrobium officinale, and tongcao, and is decocted for oral administration; Patent CN105288370A also discloses a Chinese medicine formula for lactation in pregnant women, which is prepared with Chinese medicines such as polygala, codonopsis, eucommia, white shouwu, glossy privet, saxifrage, smilax, magnolia bark, corn silk, golden silk grass, money grass, Sichuan aconite, dandelion, tongcao, malt, and is also decocted for oral administration; although both formulas add ear leaf cow skin Xiao for lactation and lactation, the formula composition is complex, and it needs to be decocted before taking, which is not only time-consuming and energy-intensive, but also difficult to control the quality and ensure the efficacy. Summary of the invention

[0003] In order to solve the above problems, the present invention provides a preparation method and application of a cervical schizonepeta composition with a lactation-promoting effect, wherein the cervical schizonepeta composition is obtained by using cervical schizonepeta as the main raw material, supplemented with ingredients such as donkey-hide gelatin, Chinese yam, and Poria cocos, and ultrasonic-assisted subcritical water technology for extraction. The preparation method has simple process from raw materials to preparations, low energy consumption, safety and environmental protection, high extraction efficiency of active ingredients in plant raw materials, and is very suitable for low-cost large-scale production; and the oral preparation made from the cervical schizonepeta composition as a raw material has good lactation-promoting effect and strong stability.

[0004] The technical solution of the present invention is: A method for preparing a rutabaga composition having a lactation-promoting effect comprises the following steps: S1: According to weight components, 10-30 parts of erye niupixiao, 16-20 parts of donkey-hide gelatin, 4-8 parts of longan, 4-8 parts of wolfberry, 10-14 parts of papaya, 8-12 parts of kudzu root, 13-15 parts of yam and 13-15 parts of tuckahoe are pretreated to obtain raw material powder; S2: mixing the raw material powder with ultrapure water at a solid-liquid ratio of 1 g: 20-40 mL, and then performing ultrasonic extraction at an ultrasonic power of 400 W, an ultrasonic temperature of 50° C., and an ultrasonic extraction time of 30-60 min to obtain an ultrasonic extract; S3: The ultrasonic extract is then extracted with subcritical water at an extraction temperature of 130° C., an extraction pressure of 4 MPa, and an extraction time of 30-50 min to obtain a Niu Pi Xiao composition.

[0005] Donkey hide gelatin can nourish blood, nourish yin, and moisten dryness; longan can nourish qi and blood, calm the mind and stabilize the spirit; wolfberry is specialized in nourishing the kidney, promoting body fluid, and invigorating qi; papaya has the effects of clearing heat, dispelling wind, and activating collaterals; kudzu root can raise yang and stop diarrhea; yam has the effects of nourishing the spleen and lungs, nourishing the kidneys and consolidating essence; and poria has the effects of promoting water and eliminating dampness, strengthening the spleen and calming the mind. The above ingredients are reasonably matched with ear leaf cowhide xiao to form a composite, and the composite is extracted to make an oral preparation, which is not only convenient to take, but also has the effects of strengthening the spleen and eliminating dampness, nourishing the liver and kidneys, and promoting milk secretion.

[0006] As a further improvement of the above scheme, the weight proportions of the raw material components in step S1 are: 20 parts of erye cow peel Xiao, 18 parts of donkey-hide gelatin, 6 parts of longan, 6 parts of wolfberry, 12 parts of papaya, 10 parts of kudzu root, 15 parts of yam, and 13 parts of Poria.

[0007] As a further improvement of the above scheme, the solid-liquid ratio of the raw material powder to the ultrapure water in step S2 is 1 g:40 mL.

[0008] As a further improvement of the above scheme, the ultrasonic extraction time in step S2 is 45 minutes.

[0009] As a further improvement of the above scheme, the extraction time of subcritical water in step S3 is 40 minutes.

[0010] As a further improvement of the above scheme, the preparation method of the Auricularia auricula composition comprises the following steps: S1: According to the weight components, 20 parts of erye niupixiao, 18 parts of donkey-hide gelatin, 6 parts of longan, 6 parts of wolfberry, 12 parts of papaya, 10 parts of kudzu root, 15 parts of yam and 13 parts of tuckahoe were mixed and pre-treated to obtain a raw material powder; S2: The raw material powder is mixed with ultrapure water at a solid-liquid ratio of 1 g:40 mL, and then ultrasonic extraction is performed. The ultrasonic power is 400 W, the ultrasonic temperature is 50° C., and the ultrasonic extraction time is 45 min to obtain an ultrasonic extract; S3: The ultrasonic extract is extracted with subcritical water at an extraction temperature of 130° C., an extraction pressure of 4 MPa, and an extraction time of 40 min to obtain a myrica pubescens composition.

[0011] As a further improvement of the above scheme, the pretreatment in step S1 is pulverization followed by screening and drying.

[0012] An application of the Auricularia auricula composition obtained by any one of the preparation methods described above, comprising using the Auricularia auricula composition as a raw material to prepare an oral preparation for lactation.

[0013] As a further improvement of the above scheme, the oral preparation is an oral liquid, which is prepared by the following method: evaporating and concentrating the ear leaf cow hide shaving composition to 30%-60% of the original volume under the conditions of a temperature of 40-65°C and a pressure of minus 0.05MPa-minus 0.10MPa to obtain an ear leaf cow hide shaving concentrate; taking 75-90 parts of the ear leaf cow hide shaving concentrate and 2-12 parts of oligoxylose, 2-6 parts of a sweetener, 1-4 parts of a flavoring agent, 1-3 parts of a preservative, and 2-5 parts of a thickener by weight, and stirring for 10-30 minutes to obtain a lactation-promoting composition; the lactation-promoting composition is filled in a bottle sterilized at high temperature, and sterilized again after filling to obtain a lactation-promoting oral liquid; The sweetener is at least one of sucrose, steviol, xylitol, mannitol, maltitol and honey; the flavoring agent is at least one of citric acid, taurine, licorice extract and menthol; the preservative is at least one of benzoic acid, sodium benzoate, sorbic acid and potassium sorbate; and the thickener is at least one of sodium carboxymethyl cellulose and hydroxypropyl methylcellulose.

[0014] As a further improvement of the above scheme, the oral preparation is a freeze-dried powder, which is prepared by the following method: the lactation-promoting oral liquid is vacuum freeze-dried at a temperature of minus 80°C to minus 40°C and a pressure of 20-80Pa to remove residual moisture and obtain a dry lactation-promoting freeze-dried powder.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: using Auricularia auricula as the main raw material, supplemented with donkey-hide gelatin, Chinese yam, Poria cocos and other ingredients, a plant raw material combination with the effects of replenishing qi and blood, regulating the spleen and stomach, and benefiting the liver and kidney is formed, and the animal experiment results show that the oral preparation prepared by the formula can improve the milk secretion ability of lactation dysfunction mother mice and has a lactation effect. In addition, the present invention adopts ultrasound-assisted subcritical water technology when extracting plant raw materials. The technology combines the cavitation effect of ultrasound and the excellent solubility of subcritical water, which can greatly improve the extraction efficiency of plant raw materials, shorten the extraction time, and avoid the use of organic solvents. Compared with traditional extraction methods, it can save costs, is safe and environmentally friendly, and has better economic benefits in large-scale production applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the following briefly introduces the drawings required for describing the embodiment. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 This is the lactation situation of each group of female mice in Test Example 1. DETAILED DESCRIPTION

[0018] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0019] The weight of the relevant components mentioned in the embodiments of the present invention description can not only refer to the specific content of each component, but also represent the proportional relationship of the weights of the components. Therefore, as long as the content of the relevant components in the embodiments of the present invention description is proportionally enlarged or reduced, it is within the scope disclosed in the embodiments of the present invention description. Specifically, the mass described in the embodiments of the present invention description can be a mass unit known in the chemical industry such as μg, mg, g, kg, etc.

[0020] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0021] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0022] Example 1 S1: According to the weight components, 20 parts of ear leaf cow hide xiao, 18 parts of donkey-hide gelatin, 6 parts of longan, 6 parts of wolfberry, 12 parts of papaya, 10 parts of kudzu root, 15 parts of yam, and 13 parts of Poria cocos plant raw materials are mixed, put into a grinder, and after passing through an 80-mesh sieve, the mixture is placed in a 50° C. oven for 12 hours to obtain a dry mixed raw material powder; S2: The mixed raw material powder obtained in step S1 is mixed with ultrapure water at a solid-liquid ratio of 1 g:30 mL, and then ultrasonic extraction is performed, the ultrasonic power is set to 400 W, the ultrasonic temperature is set to 50° C., the ultrasonic extraction time is 30 min, and then cooled to room temperature to obtain an ultrasonic extract; S3: Pour the ultrasonic extract obtained in step S2 into a subcritical reactor extraction tank, set the subcritical water extraction temperature to 130°C and the extraction pressure to 4MPa, and after extraction for 30 minutes, centrifuge the extract for filtration at a speed of 8000r / min and a centrifugation time of 10 minutes. Take the supernatant and filter it with a ceramic membrane with a pore size of 100nm, and then separate and purify it with a 600Da nanofiltration membrane to obtain the ear leaf cow hide smile composition L1.

[0023] Example 2 The components in step S1 of Example 1 were replaced with 10 parts of Auricularia auricula, 20 parts of donkey-hide gelatin, 8 parts of longan, 8 parts of wolfberry, 14 parts of papaya, 12 parts of kudzu root, 15 parts of yam, and 13 parts of Poria, and the remaining step parameters were the same as Example 1 to obtain Auricularia auricula composition L2.

[0024] Example 3 The components in step S1 of Example 1 were replaced with 30 parts of Auricularia auricula, 16 parts of donkey-hide gelatin, 4 parts of longan, 4 parts of wolfberry, 10 parts of papaya, 8 parts of kudzu root, 15 parts of yam, and 13 parts of Poria, and the remaining step parameters were the same as Example 1 to obtain Auricularia auricula composition L3.

[0025] Embodiment 4-6 The solid-liquid ratio in step S2 of Example 1 was adjusted to 1g:10mL, 1g:20mL, and 1g:40mL, respectively, and the remaining step parameters were the same as in Example 1 to obtain the Auricularia auricula composition L4-6.

[0026] Embodiment 7-9 The ultrasonic extraction time in step S2 of Example 1 was adjusted to 15 min, 45 min, and 60 min, respectively, and the remaining step parameters were the same as in Example 1 to obtain the Auricularia auricula composition L7-9.

[0027] Examples 10-12 The subcritical water extraction time in step S3 of Example 1 was adjusted to 20 min, 40 min, and 50 min, respectively, and the remaining step parameters were the same as in Example 1 to obtain the tricholoma aurantifolia composition L10-12.

[0028] Embodiment 13 Take the ear leaf cow hide xiao composition L1 obtained in Example 1 and evaporate and concentrate it to 50% of the original volume under the conditions of temperature of 55°C and pressure of -0.09 MPa to obtain ear leaf cow hide xiao concentrate; according to weight parts, 82 parts of ear leaf cow hide xiao concentrate are mixed with 6 parts of oligoxylose, 2 parts of stevioside, 2 parts of mannitol, 2 parts of citric acid, 1 part of menthol, 2 parts of potassium sorbate and 3 parts of hydroxypropyl methylcellulose, and stirred for 20 minutes to mix evenly, and filled into bottles sterilized by high temperature. After filling, sterilize again to obtain lactation oral liquid L1.

[0029] Embodiment 14 The Erye Niupixiao composition L1 was replaced with the Erye Niupixiao composition L2 obtained in Example 2, and the remaining step parameters were the same as Example 13 to obtain the lactation-promoting oral liquid L2.

[0030] Embodiment 15 The Erye Niupixiao composition L1 was replaced with the Erye Niupixiao composition L3 obtained in Example 3, and the remaining step parameters were the same as in Example 13 to obtain the lactation-promoting oral liquid L3.

[0031] Example 16 The lactation-promoting oral liquid L1 was subjected to vacuum freeze-drying treatment at a temperature of -60°C and a pressure of 30 Pa for 16 hours to remove residual moisture and obtain dry lactation-promoting freeze-dried powder L1.

[0032] Comparative Example 1 The mixed raw material powder obtained in step S1 of Example 1 was mixed with 80% ethanol at a solid-liquid ratio of 1 g:30 mL, and then refluxed for extraction. The extraction times were 3 times, and the reflux extraction time for each time was 1 hour. The filtrates were combined after filtration to obtain the Auricularia auricula composition D1.

[0033] Comparative Example 2 The mixed raw material powder obtained in step S1 of Example 1 was mixed with 80% ethanol at a solid-liquid ratio of 1 g:30 mL, and then ultrasonically extracted with an ultrasonic power of 400 W, an ultrasonic temperature of 50° C., and an ultrasonic extraction time of 60 min. After filtration, the ear leaf cowpea extract composition D2 was obtained.

[0034] Comparative Example 3 The ethanol in Comparative Example 2 was replaced by methanol, and the remaining step parameters were the same as Comparative Example 2 to prepare the Auricularia auricula composition D3.

[0035] Comparative Example 4 The mixed raw material powder obtained in step S1 of Example 1 was mixed with ultrapure water at a solid-liquid ratio of 1 g:30 mL and poured into a subcritical reactor extraction tank. The subcritical water extraction temperature was set to 130° C. and the extraction pressure was set to 4 MPa. The extraction was performed for 60 min, and then centrifuged to obtain the ear leaf cowpea extract composition D4.

[0036] Comparative Example 5 The Auricularia aurantifolia composition L1 was replaced with the Auricularia aurantifolia composition D1 obtained in Comparative Example 1, and the remaining step parameters were the same as those in Example 13 to obtain the lactation-promoting oral liquid D1.

[0037] Test Example 1: Using the content of flavonoids in the extract as an indicator to evaluate the extraction efficiency of different processes The Erye Niupixiao compositions prepared in Examples 1-12 and Comparative Examples 1-4 were tested and the total flavonoid yield was calculated according to the following method.

[0038] Weigh 21.0 mg of rutin standard accurately, dissolve it in 50% ethanol, and dilute it to a 100 mL volumetric flask to obtain a rutin standard solution with a concentration of 0.210 mg / mL. Pipette 2.0 mL, 2.5 mL, 3.0 mL, 3.5 mL, and 4.5 mL of rutin standard solution into 5 25 mL colorimetric tubes, then add 1.5 mL of 5% NaNO2 solution in turn, shake well, let stand for 5 min, add 1.5 mL of 10% Al(OH)3 solution, shake well and let stand for 5 min, add 15 mL of 10% NaOH solution, let stand for 10 min, and finally add 50% ethanol solution to dilute to 25 mL, and measure the absorbance at 510 nm. Draw a standard curve with absorbance as the ordinate and rutin mass concentration as the abscissa.

[0039] 1.0 mL was taken from the ear leaf cow hide shavings composition as the solution to be tested, diluted with 50% ethanol, fixed to a 10 mL volumetric flask, 1.0 mL of the diluted solution to be tested was drawn therefrom and transferred to a 25 mL volumetric flask, 1.5 mL of 5% NaNO2 solution was added successively, shaken well, and allowed to stand for 5 min; 1.5 mL of 10% Al(OH)3 solution was added, allowed to stand for 5 min; 15 mL of 10% NaOH solution was added, allowed to stand for 10 min, and absorbance was measured at 510 nm. 50% ethanol solution was used as a blank control solution, and the total flavonoid yield was calculated according to formula I. The specific test results are shown in Table 1 below.

[0040] Formula I: In the formula, F represents the total flavonoid yield, in mg / g; C represents the total flavonoid concentration, in mg / mL; V represents the sample solution volume, in mL; x represents the dilution multiple; and M represents the mass of the mixed raw material powder, in g.

[0041]

[0042] From the test results of total flavonoid yield in the Auricularia australis compositions obtained in Examples 1-3, it can be seen that the contents of flavonoid compounds in the extracts obtained from different plant raw material combinations are different. In the present invention, the Auricularia australis composition obtained using the raw material ratio of Example 1 has the highest content of flavonoid compounds.

[0043] From the test results of the total flavonoid yield in the Auricularia auricula composition obtained in Example 1 and Examples 4-12, it can be seen that if the ultrasonic extraction time and the subcritical water treatment time are too short, the extraction will be incomplete and the content of flavonoids in the composition will be low. If the extraction time is too long, not only will the energy consumption be large, but also the flavonoids may be decomposed, thereby deteriorating the extraction effect.

[0044] From the test results of the total flavonoids yield in the Auricularia auricula composition obtained in Example 1 and Comparative Examples 1-4, it can be seen that, compared with ethanol reflux extraction, ultrasonic ethanol extraction, ultrasonic methanol extraction and subcritical water extraction alone, the ultrasonic-assisted subcritical water technology is the most efficient way to extract plant raw materials, and it takes less time, does not require the use of organic solvents, and is safe and environmentally friendly.

[0045] Test Example 2: Effect of lactation-promoting oral solution on postpartum milk deficiency model mice Modeling and grouping of postpartum insufficiency model mice: Healthy SPF-grade healthy KM mice in lactation period with a delivery time difference of no more than 24 hours were selected and raised in a suitable environment, with free access to food and water, and a body weight range of 32±5.15g. The mice were randomly divided into 6 groups, namely blank group, model group, experimental group I, experimental group II, experimental group III and experimental group IV. The mice were given drugs starting on the first day after delivery. The drug administration method, drug administration cycle and time of each group are shown in Table 2.

[0046]

[0047] The bromocriptine solution used for administration was 0.32 mg / ml, which was obtained by diluting 2.5 mg / tablet of bromocriptine mesylate tablets with normal saline. The lactation oral solution used for administration was 15 mg / ml of lactation solution, which was obtained by diluting oral lactation solutions L1, L2, L3 and D1 with normal saline. Both the bromocriptine solution and the lactation solution were stored in brown reagent bottles at 4°C away from light.

[0048] During the experiment, the changes in litter weight of pups at different stages of the experiment were measured every day to reflect the changes in the mother's milk production. That is, the mother's hourly milk production was calculated using the weight difference of all the pups in her litter before and after one hour of breastfeeding, plus the basal metabolic rate of all the pups in the litter within one hour.

[0049] At 8 a.m. on the first day, the mother mouse was taken out for gavage, and each litter of pups was weighed for the first time, and the total weight was recorded as W1, that is, the litter weight before the pups were isolated. The pups were isolated from the mother mouse, and the pups were placed in an incubator. The mother mouse was free to eat and drink water as usual. After the pups and mother mice were isolated for 4 hours, at 12 noon, the litter weight of each litter of pups was weighed for the second time, recorded as W2, that is, the litter weight before the pups were breastfed. After weighing, each litter of pups was returned to the corresponding mother mouse for free breastfeeding for 1 hour. At 1 p.m., the mother mouse and the corresponding pups were separated again, and the total weight of each litter of pups was weighed for the last time, recorded as W3, that is, the litter weight after the pups were breastfed. After that, the mother mouse was returned to the corresponding litter of pups for free breastfeeding, and the milk volume weighing was completed. The amount of milk secreted by the mother mouse per hour = W3-W2+(W1-W2) / 4, where W1 is the total weight of each litter of pups before isolation, W2 is the weight of the litter before lactation, and W3 is the weight of the litter after lactation. Weigh the litters for 8 consecutive days, repeating the weighing process of the first day every day. The experiment ends after the weight of the litter of pups after lactation on the eighth day is weighed, and the relevant data are processed.

[0050] The results of the hourly milk production of each group of mothers are as follows: Figure 1 The results showed that the lactation of the four experimental groups was higher than that of the model group, and as the experimental days prolonged, the lactation gradually approached the lactation level of normal lactating rats, indicating that the lactation oral liquid prepared by the present invention can improve the lactation function of lactating rats, and the improvement effect of experimental group I is better than that of other experimental groups.

[0051] On the 8th day of the experiment, the weight of the mother mice, the weight of the litter of pups and the mammary gland organs of the mother mice were weighed, and the difference between the weight of the mother mice and the weight of the litter of pups on the 1st and 8th days of the experiment and the mammary gland organ index of the mother mice were calculated. The results are shown in Table 3, where the organ index = organ weight / maternal weight × 100%. The results showed that the weight of the mother mice in the model group and the experimental group was reduced to a certain extent compared with the blank group, the growth of the litter of pups was also lower, and the relative mass of the mammary gland was also lower than that of the blank group. Comparing the data of the experimental group and the model group, it can be seen that the treatment of lactation oral liquid can significantly improve the development of the maternal gland of the mother mice, reduce the weight loss caused by the mother mice during lactation, and promote the growth and development of the pups. Compared with experimental groups II-IV, the improvement effect of experimental group I is better.

[0052]

[0053] In order to further clarify the effect of prolactin oral liquid on lactation of postpartum anemic rats, Elisa kit was used to detect the content of prolactin (PRL) in the mammary gland tissue and serum of the rats in each group, and the test results are shown in Table 4. The results showed that compared with the blank group, the PRL concentration in the mammary gland and serum of the rats in the model group was significantly reduced, and the PRL level of the postpartum anemic rats was significantly increased after intervention with prolactin oral liquid, among which the improvement effect of experimental group I was the best.

[0054]

[0055] Test Example 3: Effect of natural light at room temperature on the content of flavonoids in lactation products After the lactation-promoting oral liquid L1 obtained in Example 13 and the lactation-promoting freeze-dried powder L1 obtained in Example 16 were placed at room temperature under natural light for a certain period of time, the content of flavonoid compounds in the samples was detected. The test results are shown in Table 5.

[0056] The results showed that the content of total flavonoids in the sample decreased with the extension of storage time, but it could still maintain more than 90% after 8 months, indicating that the flavonoids in the lactation oral liquid and freeze-dried powder prepared by the present invention have good stability. Moreover, compared with the oral liquid, the content of flavonoids in the freeze-dried powder only changed slightly, indicating that the freeze-dried powder dosage form of the ear leaf cow skin Xiao composition is more suitable for long-term storage.

[0057]

[0058] Test Example 4: Effect of natural light at room temperature on the content of microorganisms in lactation oral solution According to the "General Principles of Microbiological Examination of Foods in National Food Safety Standards" (GB 4789.1-2016) and the "Microbiological Examination Methods" in the "General Principles of Microbiological Examination of Food Hygiene", the total bacteria count, total mold / yeast count, Staphylococcus aureus, Salmonella, and heat-resistant coliform group content of the lactation oral solution L1 obtained in Example 13 after being placed for a certain period of time were tested, and the test results are shown in Table 6. The results show that after being placed for 1-4 months, the content of each microorganism in the lactation oral solution L1 meets the food safety technical specifications, and there is no excess phenomenon, indicating that the product is safe to eat.

[0059]

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of this application should be included in the protection scope of this application.

Claims

1. A method for preparing a composition of Auricularia auricula having a lactogenic effect, characterized in that: The following steps are involved: S1: According to weight components, 10-30 parts of erye niupixiao, 16-20 parts of donkey-hide gelatin, 4-8 parts of longan, 4-8 parts of wolfberry, 10-14 parts of papaya, 8-12 parts of kudzu root, 13-15 parts of yam and 13-15 parts of tuckahoe are pretreated to obtain raw material powder; S2: mixing the raw material powder with ultrapure water at a solid-liquid ratio of 1 g: 20-40 mL, and then performing ultrasonic extraction at an ultrasonic power of 400 W, an ultrasonic temperature of 50° C., and an ultrasonic extraction time of 30-60 min to obtain an ultrasonic extract; S3: The ultrasonic extract is then extracted with subcritical water at an extraction temperature of 130° C., an extraction pressure of 4 MPa, and an extraction time of 30-50 min to obtain a Niu Pi Xiao composition.

2. The preparation method according to claim 1, characterized in that The weight proportions of the raw material components in step S1 are: 20 parts of erye cow peel xiao, 18 parts of donkey-hide gelatin, 6 parts of longan, 6 parts of wolfberry, 12 parts of papaya, 10 parts of kudzu root, 15 parts of yam, and 13 parts of poria.

3. The preparation method according to claim 1, characterized in that: The solid-to-liquid ratio of the raw material powder to ultrapure water in step S2 is 1 g:40 mL.

4. The preparation method according to claim 1, characterized in that: The ultrasonic extraction time in step S2 is 45 minutes.

5. The preparation method according to claim 1, characterized in that: The extraction time of subcritical water in step S3 is 40 min.

6. The preparation method according to claim 1, characterized in that: The following steps are involved: S1: According to the weight components, 20 parts of erye niupixiao, 18 parts of donkey-hide gelatin, 6 parts of longan, 6 parts of wolfberry, 12 parts of papaya, 10 parts of kudzu root, 15 parts of yam and 13 parts of tuckahoe were mixed and pre-treated to obtain a raw material powder; S2: The raw material powder is mixed with ultrapure water at a solid-liquid ratio of 1 g:40 mL, and then ultrasonic extraction is performed. The ultrasonic power is 400 W, the ultrasonic temperature is 50° C., and the ultrasonic extraction time is 45 min to obtain an ultrasonic extract; S3: The ultrasonic extract is then extracted with subcritical water at an extraction temperature of 130° C., an extraction pressure of 4 MPa, and an extraction time of 40 min to obtain a Niu Pi Xiao composition.

7. An application of the Auricularia auricula composition obtained by the preparation method according to any one of claims 1 to 6, characterized in that: The invention comprises taking the ear leaf cow peel xiao composition as a raw material to prepare an oral preparation for lactation.

8. The use according to claim 7, characterized in that The oral preparation is an oral liquid.

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