Pretreatment diluent for detecting capsaicin in illegal cooking oil and application of pretreatment diluent

Abstract

The invention provides a pretreatment diluent for detecting capsaicin in illegal cooking oil and application of the pretreatment diluent. The pretreatment diluent comprises the following components in mass concentration: 0.1%-20% of a surfactant, 0.1%-1% of lauryl sodium sulfate and 1%-5% of Proclin300. The surface active agent is a combination of triton X-100 and Tween 20. The pretreatment diluent comprises a surfactant, a detergent and a preservative, proper compound types and concentrations are screened out, the illegal cooking oil pretreatment diluent suitable for capsaicin detection is prepared, the pretreatment diluent can be used for capsaicin rapid detection test paper, and on-site rapid detection of illegal cooking oil is achieved.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a pre-treatment diluent for detecting capsaicin in waste cooking oil and application thereof. Background Art

[0002] Gutter oil is a general term for various inferior oils in human life and production, mainly including waste oils from the catering industry and inferior oils processed from various animal meats and offal. Different processing processes will produce toxic and harmful components such as heavy metal impurities, oil oxides, and aflatoxin. In order to make huge profits, some lawless elements secretly use it directly as cooking oil or add it to other edible vegetable oils for sale and use, which brings huge safety hazards to the lives and health of the general public.

[0003] At present, many new detection methods have been introduced into the detection technology of waste oil. The detection basis is mainly based on the difference in physical properties or chemical properties. There are two main trends in waste oil detection. One is a simple and convenient detection method for simply processed waste oil; the other is a high-precision and high-sensitivity detection method for deeply processed waste oil. In order to meet the needs of rapid identification of edible oil, the common properties of waste oil are studied, and a pre-dilution solution for waste oil detection is established to improve the speed of rapid identification of waste oil, which has broad application prospects.

[0004] Because waste oil is through precipitation, decolorization, deodorization, the waste oil after refining adopts sensory recognition method and can no longer be judged, and pepper is added in the cooking process as common seasoning, and capsaicin can be not completely destroyed and dissolved in oil in the cooking process as the effective ingredient of pepper. Therefore, capsaicin is used as a kind of standard for distinguishing waste oil, and waste oil contains a large amount of grease and can not be directly used as sample for capsaicin rapid detection test paper, and waste oil is processed into can be directly used for waste oil rapid detection by pre-diluent, improves waste oil on-site detection speed. But there is no efficient and accurate diluent for waste oil pre-treatment at present, therefore, a kind of diluent for waste oil detection is provided and has important application value in the detection of waste oil. Summary of the invention

[0005] In view of the shortcomings of the prior art, the object of the present invention is to provide a pre-treatment diluent for detecting capsaicin in waste oil and its application. The pre-treatment diluent of the present invention comprises a surfactant, a detergent, and a preservative, and screens out suitable compound types and concentrations, and prepares a waste oil diluent suitable for detecting capsaicin, which can be used for a rapid detection test paper for capsaicin, and realizes on-site rapid detection of capsaicin in waste oil.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a pre-treatment dilution for detecting capsaicin in waste cooking oil, wherein the pre-treatment dilution comprises the following components in mass concentrations: 0.1%-20% surfactant, 0.1%-1% sodium dodecyl sulfate and 1%-5% Proclin300;

[0008] The surfactant is a combination of Triton X-100 and Tween 20.

[0009] Among them, 0.1%-20% surfactant can be, for example, 0.1%, 0.5%, 1%, 5%, 10%, 15% or 20%; 0.1%-1% sodium dodecyl sulfate can be, for example, 0.1%, 0.3%, 0.5%, 0.7%, 0.9% or 1%; 1%-5% Proclin300 can be, for example, 1%, 2%, 3%, 4% or 5%.

[0010] The pretreatment diluent for detecting capsaicin in waste cooking oil of the present invention comprises a surfactant, a detergent and a preservative; wherein the surfactant is a combination of Triton X-100 and Tween 20, the detergent is sodium dodecyl sulfate, and the preservative is Proclin 300.

[0011] In the present invention, Triton X-100 is a nonionic surfactant with a solubilizing effect, and Tween 20 is a nonionic surfactant with a solubilizing and stabilizing effect. In the present invention, the Triton X-100 and Tween 20 cooperate with each other and have a synergistic effect, can promote the dissolution of waste oil, promote the release of capsaicin, maintain the stability of the sample to be tested, and effectively increase the accuracy and sensitivity of the detection.

[0012] In the present invention, sodium dodecyl sulfate is specifically selected as the detergent. Sodium dodecyl sulfate has good water solubility, stability in aqueous solutions with a wide pH value, and is a surfactant with strong degreasing ability. The detergent and surfactant can also cooperate with each other to enhance synergy.

[0013] In the present invention, Proclin 300 is specifically selected as a preservative. It is a highly effective antibacterial agent with broad-spectrum antibacterial activity, excellent compatibility and stability, and low toxicity at the concentration used. It is harmless to health within the recommended concentration and has the characteristics of a wide applicable pH range and good water solubility.

[0014] Preferably, the mass concentration of Triton X-100 is 5%-20%.

[0015] In the present invention, when the mass concentration of Triton X-100 is within the range of 5%-20%, the solubility of capsaicin in the solution can be increased. When the mass concentration is too high or too low, the solubility of capsaicin in the solution is reduced or the stripping is affected.

[0016] In a preferred embodiment of the present invention, the mass concentration of Triton X-100 is 10%.

[0017] Preferably, the mass concentration of Tween 20 is 0.1%-1%.

[0018] In the present invention, when the mass concentration of Tween 20 is within the range of 0.1%-1%, the solubility of capsaicin in the solution can be increased; when the mass concentration is too high or too low, the solubility of capsaicin in the solution is reduced, affecting the detection effect.

[0019] In a preferred embodiment of the present invention, the mass concentration of Tween 20 is 0.5%.

[0020] Preferably, the pre-treatment diluent comprises the following components in mass concentrations: 5%-20% Triton X-100, 0.1%-1% sodium dodecyl sulfate, 0.1%-1% Tween 20 and 1%-5% Proclin 300, and the solvent is water.

[0021] In a second aspect, the present invention provides a method for detecting capsaicin in waste cooking oil, the method comprising: mixing the pre-treatment diluent for detecting capsaicin in waste cooking oil described in the first aspect with the oil to be tested to obtain a sample solution to be tested, and then using a test paper to detect the sample solution to be tested.

[0022] Preferably, the volume ratio of the pre-treatment diluent to the oil to be tested is (8-12):1, for example, it can be 8:1, 9:1, 10:1, 11:1 or 12:1.

[0023] In the present invention, the volume ratio of the pretreatment diluent to the oil to be tested affects the dissolution of capsaicin. Under the above ratio conditions, the detection sensitivity of capsaicin in waste oil can be improved. Exceeding the above ratio range affects the dissolution of capsaicin and reduces the detection sensitivity of capsaicin in waste oil.

[0024] Preferably, the mixing condition is 20-30°C, for example, 20°C, 25°C or 30°C.

[0025] In the present invention, the pre-treatment diluent and the oil to be tested can be mixed at room temperature, and the treatment conditions are mild, simple, safe and efficient.

[0026] In a third aspect, the present invention provides a kit for detecting capsaicin in waste cooking oil, the kit comprising the pre-treatment dilution solution for detecting capsaicin in waste cooking oil according to the first aspect.

[0027] Preferably, the kit further comprises a colloidal gold test strip; the colloidal gold test strip comprises a bottom plate, on which a sample pad, a gold label pad, a nitrocellulose membrane and absorbent paper are arranged; a C detection line and a T detection line are arranged on the nitrocellulose membrane; the gold label pad contains a capsaicin antibody-colloidal gold marker; the C detection line is coated with goat anti-rabbit IgG; and the T detection line is coated with a capsaicin-antigen hemoglobin conjugate.

[0028] In a fourth aspect, the present invention provides the use of any one or a combination of at least two of the pretreatment diluent for detecting capsaicin in waste oil described in the first aspect, the method for detecting capsaicin in waste oil described in the second aspect, or the kit for detecting capsaicin in waste oil described in the third aspect in detecting the content of capsaicin in oils and fats.

[0029] The numerical range described in the present invention not only includes the point values ​​listed above, but also includes any point values ​​between the above numerical ranges that are not listed. Due to space limitations and for the sake of simplicity, the present invention no longer exhaustively lists the specific point values ​​included in the range.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) In the present invention, after the waste cooking oil is treated with a pre-treatment diluent, a sample solution to be tested that can be directly used for rapid detection is obtained, thereby improving the on-site detection speed of capsaicin in waste cooking oil.

[0032] (2) The pretreatment diluent of the present invention comprises a surfactant, a detergent and a preservative, and the above components cooperate with each other to have a synergistic effect; the present invention can improve the solubility of waste cooking oil, increase the stability of capsaicin detection reagent, reduce the difference in reagent results, and at the same time improve the accuracy and sensitivity of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a reference color chart. DETAILED DESCRIPTION

[0034] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0035] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0036] Example 1

[0037] This embodiment provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 0.5% Tween 20, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0038] Example 2

[0039] This embodiment provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 1% Tween 20, 1% sodium dodecyl sulfate, 5% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0040] Example 3

[0041] This embodiment provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 0.1% Tween 20, 0.1% sodium dodecyl sulfate, 1% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0042] Example 4

[0043] This embodiment provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 5% Triton X-100, 0.5% Tween 20, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0044] Example 5

[0045] This embodiment provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 20% Triton X-100, 0.5% Tween 20, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0046] Comparative Example 1

[0047] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 30% of Triton X-100, 0.5% of Tween 20, 0.5% of sodium dodecyl sulfate, and 2% of Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried, and stored.

[0048] Comparative Example 2

[0049] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 1% Triton X-100, 0.5% Tween 20, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0050] Comparative Example 3

[0051] This comparative example provides a pre-treatment dilution for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment dilution is as follows: 10% Triton X-100, 1% Tween 20, 2% Proclin 300, and the solvent is water. The dilution is fully dissolved and stirred, then canned, sealed, dried and stored.

[0052] Comparative Example 4

[0053] This comparative example provides a pre-treatment dilution for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment dilution is as follows: 10% of Triton X-100, 1% of sodium lauryl sulfate, and 2% of Proclin 300, and the solvent is water. The dilution is fully dissolved and stirred, then canned, sealed, dried, and stored.

[0054] Comparative Example 5

[0055] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 3% Tween 20, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0056] Comparative Example 6

[0057] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 0.5% Tween 20, 3% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0058] Comparative Example 7

[0059] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% surfactant S9, 0.5% Tween 20, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0060] Comparative Example 8

[0061] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste cooking oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 0.5% EDTA, 0.5% sodium dodecyl sulfate, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0062] Comparative Example 9

[0063] This comparative example provides a pre-treatment diluent for detecting capsaicin in waste oil, wherein the formula of the pre-treatment diluent is as follows: 10% Triton X-100, 0.5% Tween 20, 0.5% PVP, 2% Proclin 300, and the solvent is water. The diluent is fully dissolved and stirred, then canned, sealed, dried and stored.

[0064] Test Example 1

[0065] This test example tests the effect of the pre-treatment dilution solution used for the detection of capsaicin in waste cooking oil.

[0066] 1. Preparation of colloidal gold detection kit for detecting capsaicin in waste oil

[0067] (1) Preparation of capsaicin colloidal gold label

[0068] Preparation of colloidal gold solution:

[0069] Colloidal gold particles were prepared by the trisodium citrate reduction method. A clean magnetic rotor was placed in a suitable clean conical flask. A certain amount of deionized water was accurately weighed into the conical flask. The conical flask was placed on a heated stirrer and boiled. Stirring was accelerated, and then an appropriate amount of chloroauric acid solution and an appropriate amount of trisodium citrate solution were immediately added. When the color of the solution changed from clear and colorless to translucent wine red, the accelerated stirring was continued for 5 minutes and then the heating was stopped. The residual heat of the instrument was used to continue stirring for a few minutes and then cooled to room temperature. The solution was then stored in a refrigerator at 4°C for later use.

[0070] Preparation of capsaicin antibody-colloidal gold label:

[0071] Take 30mL of the colloidal gold solution prepared above and put it into a 100mL beaker with a clean rotor, put the beaker on the stirrer and stir for 10min, then accurately draw 300μL of buffer solution to adjust the pH and stir for 20-30min. Take 1mL of colloidal gold solution and place it in a 2mL centrifuge tube, add 10μL of antibody (mouse anti-capsaicin monoclonal antibody, Balb / c mouse, item number: LD-Ab0068), observe the color of the marked solution at room temperature, if the color is normal, then add a certain amount of antibody, stir and react for 30min, then add 300μL of BSA blocking agent solution, after 30min of reaction, put the solution into the centrifuge tube in equal amounts, pay attention to the alignment when placing the centrifuge tube, 11500r / min, 15min, pour out the supernatant as soon as the centrifuge stops, and finally collect the remaining solution into a centrifuge tube with a pipette. Finally, dilute the collected solution with purified water in equal proportions, measure OD with a UV detector, and record it.

[0072] (2) Sample pad processing

[0073] Dissolve the prepared sample pad diluent fully, stir evenly and spray it on the glass fiber, place it in an oven at 37°C to dry for 24 hours, take it out, seal it and store it dry.

[0074] (3) Preparation of gold label pad

[0075] The colloidal gold-labeled monoclonal antibody and labeled rabbit IgG (Qingdao Henderson Biotechnology Co., Ltd., catalog number: HDS-21045) were diluted with PG2 solution to a concentration of OD values ​​of 30 and 20, and appropriate amounts of trehalose and sucrose were added. The mixture was oscillated and then sprayed on the treated capsaicin hair gold-labeled fiberglass pad using a gold sprayer. The mixture was then placed in a 37°C oven to dry for 20 hours and then sealed and stored away from light.

[0076] (4) Coating membrane preparation

[0077] The capsaicin-antigen hemoglobin conjugate and goat anti-rabbit IgG were diluted to 0.5 mg / mL and 1.0 mg / mL using PBS diluent using coating buffer, and the concentration was prepared to 1.0 mg / mL. After the preparation, the above goat anti-rabbit IgG diluent and capsaicin-antigen hemoglobin conjugate were coated on the quality control line and test line on the NC membrane at a coating amount of 1.0 μL / cm, respectively, and then placed in a 45°C oven to dry for 24 hours and then sealed and stored at room temperature.

[0078] (5) Assembly

[0079] On the PVC base plate, the sample pad, gold label pad, NC membrane, absorbent paper and other accessories are attached in order as required. The absorbent pad is attached to the upper part of the nitrocellulose membrane, resulting in a partial overlap of 1-2 mm. The lower end of the nitrocellulose membrane is placed under the fluorescent pad, with a partial overlap of 1-2 mm between the two. The other end of the fluorescent pad overlaps the sample pad by 1-2 mm, and the other end of the sample pad is aligned with the PVC liner. After assembly, cut into reagent strips of about 4 mm, put into the reagent card and press tightly for use.

[0080] 2. Testing

[0081] The diluent and the oil to be tested in the above embodiment and comparative example are mixed in a ratio of 10:1. The oil to be tested includes qualified soybean oil, soybean oil treated with the diluent, waste cooking oil, and waste cooking oil treated with the diluent. Each is repeated 5 times. The sample solution to be tested is drawn with a dropper and 3 drops are added to the sample addition hole of the reagent plate. The color development of the C and T lines of the test strip is observed for 3-5 minutes. Refer to the color development table as shown in Figure 1 The recorded color intensity is shown in Table 1.

[0082] Table 1

[0083]

[0084]

[0085]

[0086] The above reagent strip uses a strip-shaped fiber layer material with fixed detection lines and quality control lines as the stationary phase, a test liquid as the mobile phase, colloidal gold-labeled antibodies or antigens fixed on the connection pad, and the analyte moves on the chromatography strip through capillary action.

[0087] The oil sample treated with the pre-treatment diluent is dripped into the sample well, and the sample is then chromatographed upward under the capillary effect.

[0088] If the result is negative, the colloidal gold antibody cannot be completely bound to capsaicin. The colloidal gold antibody will be bound to the capsaicin conjugate fixed on the membrane during the chromatography process, and a purple-red strip will appear in the test area (T).

[0089] If it is positive, the colloidal gold antibody will be completely combined with capsaicin, so that no purple-red strip will appear in the test area (T) due to the competitive reaction. Negative samples will have purple-red strips in the test area (T) due to the lack of antibody-antigen competitive reaction during the test.

[0090] Regardless of whether capsaicin is present in the sample, a purple-red band will appear in the quality control area (C). The purple-red band in the quality control area (C) is the standard for judging whether there is enough sample and whether the chromatography process is normal, and it also serves as the internal control standard of the reagent.

[0091] In Examples 1-5, qualified soybean oil and waste oil were added dropwise on the capsaicin detection reagent strip for 3-5 minutes without any color shift. The qualified soybean oil treated with the diluent developed a color of G10 on the capsaicin detection reagent strip for 3-5 minutes, with a relatively strong color development. The waste oil treated with the diluent developed a color of G4 on the capsaicin detection reagent strip for 3-5 minutes. The qualified soybean oil treated with the diluent and the waste oil treated with the diluent had an obvious color gradient, indicating that the waste oil diluent of the present invention can clearly identify the waste oil component in the prepared capsaicin detection reagent.

[0092] In Comparative Examples 1-2, qualified soybean oil and waste cooking oil were added dropwise on the capsaicin detection reagent strip for 3-5 minutes without any strip run; qualified soybean oil treated with the diluent was added dropwise on the capsaicin detection reagent strip for 3-5 minutes, and comparative example 1 developed color G8-9, and comparative example 2 developed color G8; waste cooking oil treated with the diluent was added dropwise on the capsaicin detection reagent strip for 3-5 minutes, and comparative example 1 developed color G7, and comparative example 2 developed color G7; in Comparative Examples 1 and 2, the content of Triton X-100 was too high or too low, and the treatment effect of the prepared diluent was poor, indicating that when the content of Triton X-100 was too high or too low, the solubility of capsaicin in the diluent was affected.

[0093] In Comparative Examples 3-4, qualified soybean oil and waste cooking oil were dripped on the capsaicin detection reagent strip for 3-5 minutes without running; qualified soybean oil treated with the diluent was dripped on the capsaicin detection reagent strip for 3-5 minutes, and Comparative Example 3 developed color G9, and Comparative Example 4 developed color G9; waste cooking oil treated with the diluent was dripped on the capsaicin detection reagent strip for 3-5 minutes, and Comparative Example 3 developed color G7, and Comparative Example 4 developed color G7-8; Comparative Example 3 did not contain sodium dodecyl sulfate, and Comparative Example 4 did not contain Tween 20, and the treatment effect of the prepared diluent was poor, indicating that sodium dodecyl sulfate and Tween 20 would reduce the solubility of capsaicin.

[0094] In Comparative Examples 5 and 6, qualified soybean oil and waste cooking oil were dripped on the capsaicin detection reagent strip for 3-5 minutes without running; qualified soybean oil treated with the diluent was dripped on the capsaicin detection reagent strip for 3-5 minutes, and Comparative Example 5 developed color G9, and Comparative Example 6 developed color G9; waste cooking oil treated with the diluent was dripped on the capsaicin detection reagent strip for 3-5 minutes, and Comparative Example 5 developed color G7-8, and Comparative Example 6 developed color G7-8; the content of Tween 20 in Comparative Example 5 was too high, and the content of sodium dodecyl sulfate in Comparative Example 6 was too high, and the treatment effect of the prepared diluent was poor, indicating that the content of sodium dodecyl sulfate and Tween 20 was too high, which would reduce the solubility of capsaicin.

[0095] In comparative examples 7-9, qualified soybean oil and waste oil were dripped on the capsaicin detection reagent strip for 3-5 minutes without running the strip; qualified soybean oil treated with the diluent was dripped on the capsaicin detection reagent strip for 3-5 minutes, and comparative example 7 developed color G5, comparative example 8 developed color G5, and comparative example 9 developed color G5; waste oil treated with the diluent was dripped on the capsaicin detection reagent strip for 3-5 minutes, comparative example 7 developed color G4, comparative example 8 developed color G7-8, and comparative example 9 developed color G7; in comparative example 7, surfactant 9 was used to replace Triton X-100, in comparative example 8, EDTA was used to replace Tween 20, and in comparative example 9, PVP was used to replace sodium dodecyl sulfate, and the treatment effect of the prepared diluent was poor, indicating that Triton X-100, SDS, and Tween 20 have a synergistic effect in promoting the color intensity and specific binding efficacy of capsaicin in waste oil, and indicating that the diluent designed by the present invention can promote the specific binding of antigen and antibody and reduce other non-specific interference.

[0096] In summary, the use of the waste oil pretreatment diluent in the present invention can improve the solubility of the waste oil, increase the stability of the capsaicin detection reagent, reduce the difference in reagent results, speed up the reaction time, and improve the detection effect.

[0097] The applicant declares that the above is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention shall fall within the protection scope and disclosure scope of the present invention.

Claims

1. A pre-treatment diluent for detecting capsaicin in waste oil, characterized in that: The pre-treatment diluent comprises the following components in mass concentrations: 0.1%-20% surfactant, 0.1%-1% sodium dodecyl sulfate and 1%-5% Proclin300; The surfactant is a combination of Triton X-100 and Tween 20.

2. The pre-treatment diluent for detecting capsaicin in waste oil according to claim 1, characterized in that: The mass concentration of the Triton X-100 is 5%-20%.

3. The pre-treatment diluent for detecting capsaicin in waste oil according to claim 2, characterized in that: The mass concentration of Tween 20 is 0.1%-1%.

4. The pre-treatment diluent for detecting capsaicin in waste oil according to any one of claims 1 to 3, characterized in that: The pre-treatment diluent comprises the following components in mass concentrations: 5%-20% Triton X-100, 0.1%-1% sodium dodecyl sulfate, 0.1%-1% Tween 20 and 1%-5% Proclin 300, and the solvent is water.

5. A method for detecting capsaicin in waste oil, characterized in that: The method comprises: mixing the pre-treatment dilution for detecting capsaicin in waste oil according to any one of claims 1 to 4 with the oil to be tested to obtain a sample solution to be tested, and then using a test paper to detect the sample solution to be tested.

6. The method for detecting capsaicin in waste oil according to claim 5, characterized in that: The volume ratio of the pre-treatment diluent to the oil to be tested is (8-12):

1.

7. The method for detecting capsaicin in waste oil according to claim 5 or 6, characterized in that: The mixing condition is 20-30°C.

8. A kit for detecting capsaicin in waste oil, characterized in that: The kit comprises the pre-treatment diluent for waste cooking oil detection according to any one of claims 1 to 4.

9. The kit for detecting capsaicin in waste oil according to claim 8, characterized in that: The test kit also includes a colloidal gold test strip; the colloidal gold test strip includes a bottom plate, on which a sample pad, a gold label pad, a nitrocellulose membrane and absorbent paper are arranged; a C detection line and a T detection line are arranged on the nitrocellulose membrane; the gold label pad contains a capsaicin antibody-colloidal gold marker; the C detection line is coated with goat anti-rabbit IgG; and the T detection line is coated with a capsaicin-antigen hemoglobin conjugate.

10. Use of any one or a combination of at least two of the pre-treatment diluent for detecting capsaicin in waste oil according to any one of claims 1-4, the method for detecting capsaicin in waste oil according to any one of claims 5-7, or the kit for detecting capsaicin in waste oil according to claim 8 or 9 in detecting the content of capsaicin in oil.

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