Preparation method of scutellaria baicalensis stem and leaf extract preparation

Abstract

The invention belongs to the technical field of veterinary medicine preparation, and particularly relates to a preparation method of a scutellaria baicalensis stem and leaf extract preparation. The preparation method of the scutellaria baicalensis stem and leaf extract preparation comprises the following steps: performing alcohol extraction on scutellaria baicalensis stems and leaves to obtain an alcohol extract; and uniformly mixing the alcohol extract and the lauryl sodium sulfate / polyethylene glycol-6000 mixture to obtain the scutellaria baicalensis stem and leaf extract preparation. According to the preparation method, lauryl sodium sulfate and polyethylene glycol-6000 are jointly added into a scutellaria baicalensis stem and leaf extract obtained through an alcohol extraction method, and the scutellaria baicalensis stem and leaf extract preparation is prepared from the scutellaria baicalensis stem and leaf extract. Due to the solubilizing characteristic of the lauryl sodium sulfate and the polyethylene glycol-6000, the lauryl sodium sulfate and the polyethylene glycol-6000 are dissolved in water and then gather in water to form micelles, the scutellaria baicalensis stem and leaf extract which is insoluble in water can be wrapped in a micelle core through the method, and the solubility of the scutellaria baicalensis stem and leaf extract is further improved.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary medicine preparation, and particularly relates to a method for preparing a scutellaria baicalensis stem and leaf extract preparation. Background Art

[0002] Scutellaria baicalensis, also known as camellia root and earthy tea root, is a perennial herbaceous plant of the genus Scutellaria in the Lamiaceae family. It is a common Chinese medicinal material. The Chinese Veterinary Pharmacopoeia and the Chinese Pharmacopoeia stipulate that its medicinal part is the root of the Scutellaria baicalensis plant, and its growth cycle is 3-4 years. The stems and leaves of Scutellaria baicalensis are the above-ground parts of the Scutellaria baicalensis plant. Compared with the medicinal materials of Scutellaria baicalensis, the chemical composition of the two is quite different. The main active ingredients in the medicinal materials of Scutellaria baicalensis are baicalin, baicalein, wogonin, baicalein, etc., while the main ingredients in the stems and leaves of Scutellaria baicalensis are baicalin, taxifolin, isoschaftoside, etc. As a traditional Chinese medicine, the root of Scutellaria baicalensis has the effect of clearing away heat and detoxifying. Studies have found that the stems and leaves of Scutellaria baicalensis, as the non-medicinal parts of Scutellaria baicalensis, also have significant antibacterial, anti-inflammatory and antiviral effects. In terms of livestock and poultry disease prevention and control, they have shown better effects than Scutellaria baicalensis. At the same time, they are inexpensive and are particularly suitable for use in the breeding industry.

[0003] Since the main medicinal ingredients of Scutellaria baicalensis stems and leaves need to be extracted using ethanol, the Scutellaria baicalensis stem and leaf extract formed by the alcohol extraction process has poor water solubility, and the current main method of administration for large-scale farmed animals is drinking water, this has limited the application of Scutellaria baicalensis stem and leaf extract in large-scale farmed animals.

[0004] Therefore, there is an urgent need to provide a new method for preparing a scutellaria stem and leaf extract to solve the problem of poor water solubility of the scutellaria stem and leaf alcohol extract. Summary of the invention

[0005] The invention aims to provide a method for preparing a scutellaria stem and leaf extract preparation, which solves the problem that the scutellaria stem and leaf alcohol extract is difficult to dissolve in water.

[0006] The technical solution adopted by the present invention is: the present invention provides a method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0007] The stems and leaves of Scutellaria baicalensis are subjected to alcohol extraction to obtain an alcohol extract.

[0008] The alcohol extract and the sodium dodecyl sulfate / polyethylene glycol-6000 mixture are evenly mixed to obtain a Scutellaria baicalensis stem and leaf extract preparation, wherein the weight ratio of the alcohol extract to the sodium dodecyl sulfate / polyethylene glycol-6000 mixture is 50:2-3, and the sodium dodecyl sulfate / polyethylene glycol-6000 mixture is prepared by mixing sodium dodecyl sulfate and polyethylene glycol-6000 in a weight ratio of 1-3:1.

[0009] Preferably, the weight ratio of the alcohol extract to the sodium lauryl sulfate / polyethylene glycol-6000 mixture is 25:1.

[0010] Preferably, the weight ratio of sodium lauryl sulfate to polyethylene glycol-6000 is 3:1.

[0011] Preferably, the uniform mixing time is 30 min to 35 min.

[0012] The present invention also provides a method for preparing an alcohol extract, comprising the following steps:

[0013] The dried and crushed scutellaria stem and leaf samples are weighed, 50% to 70% ethanol by volume is added for reflux alcohol extraction, the extract is collected, the extract is centrifuged, the medicinal liquid is collected, the medicinal liquid is concentrated and dried, and cooled to room temperature to obtain an alcohol extract; the solid-liquid ratio of the scutellaria stem and leaf samples to ethanol is 1:20 to 30.

[0014] Preferably, the solid-liquid ratio of the Scutellaria baicalensis stem and leaf sample to ethanol is 1:20.

[0015] Preferably, the reflux alcohol extraction is performed once to twice.

[0016] Preferably, the reflux alcohol extraction time is 30 min to 70 min.

[0017] Preferably, the temperature of the concentrated drying is 100°C to 105°C.

[0018] Compared with the prior art, the present invention has the following beneficial effects: in order to solve the problem of poor water solubility of the alcohol extract of scutellaria stem and leaf, the present invention provides a method for preparing a scutellaria stem and leaf extract preparation, comprising the following steps: performing alcohol extraction on scutellaria stem and leaf to obtain an alcohol extract; and uniformly mixing the alcohol extract with a mixture of sodium dodecyl sulfate / polyethylene glycol-6000 to prepare a scutellaria stem and leaf extract preparation. The weight ratio of the alcohol extract to the mixture of sodium dodecyl sulfate / polyethylene glycol-6000 is 50:2-3, the mixture of sodium dodecyl sulfate / polyethylene glycol-6000 is a mixture of sodium dodecyl sulfate and polyethylene glycol-6000, and the weight ratio of sodium dodecyl sulfate to polyethylene glycol-6000 is 1-3:1.

[0019] Sodium dodecyl sulfate is a type of anionic surfactant with the characteristics of dispersion and solubilization; polyethylene glycol 6000, as a water-soluble high molecular polymer solid excipient, has significant dispersibility for poorly soluble drugs in water. The present invention prepares the scutellaria stem and leaf extract into a scutellaria stem and leaf extract preparation by adding the two together to the scutellaria stem and leaf extract obtained by alcohol extraction. Since sodium dodecyl sulfate aggregates in water to form micelles, the poorly soluble scutellaria stem and leaf extract can be encapsulated in the micelle core to further increase the solubility of the scutellaria stem and leaf extract.

[0020] The present invention adopts the combination of polyethylene glycol-6000 and sodium dodecyl sulfate to solve the problem of poor water solubility of the alcohol extract of Scutellaria baicalensis stems and leaves, which also lays a foundation for drinking water medication for livestock and poultry, reduces the waste of manpower and material resources in the medication process, and improves breeding efficiency. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments.

[0022] In the description of the present invention, unless otherwise specified, all reagents used are commercially available and all methods used are conventional techniques in the art.

[0023] The inventive concept of the present invention is as follows:

[0024] The main active ingredients of Scutellaria baicalensis stems and leaves need to be extracted using ethanol, but the Scutellaria baicalensis stem and leaf extract formed by the alcohol extraction process has poor water solubility. Currently, the main method of administering drugs to animals after large-scale breeding is drinking water, which strictly limits the application of Scutellaria baicalensis stem and leaf extract.

[0025] The pharmaceutical excipient polyethylene glycol 6000 is a mixture formed by the condensation of ethylene oxide and water. It is a water-soluble high molecular polymer solid excipient. It has significant dispersibility for poorly soluble drugs in water. Because it is non-toxic and non-irritating, it is usually used as a matrix material for pharmaceutical preparations such as pills, suppositories, ointments, tablets and capsules, etc., which can significantly improve the dissolution rate and bioavailability of drugs.

[0026] Sodium dodecyl sulfate is a type of anionic surfactant that is easily soluble in water and has the functions of dispersing, solubilizing, emulsifying, defoaming, lubricating, washing, preserving and sterilizing. Sodium dodecyl sulfate aggregates in water to form micelles. In pharmaceutical preparations, it often encapsulates some poorly soluble drugs in the micelle core to increase the solubility of the drug, prolong the drug action time, and improve the bioavailability of the drug.

[0027] The present invention combines polyethylene glycol 6000 for dispersing poorly soluble drugs with sodium dodecyl sulfate for encapsulating poorly soluble drugs in water to solve the technical problem that the scutellaria stem and leaf extract is poorly soluble in water. Based on this, the present invention provides a method for preparing a scutellaria stem and leaf extract preparation, and the method is as follows:

[0028] The stems and leaves of Scutellaria baicalensis are subjected to alcohol extraction to obtain an alcohol extract; the alcohol extract and a mixture of sodium dodecyl sulfate / polyethylene glycol-6000 are evenly mixed to prepare a Scutellaria baicalensis stem and leaf extract preparation.

[0029] The present invention is described in detail below with reference to specific embodiments.

[0030] The reagents and drugs used in the present invention: polyethylene glycol-6000 (PEG-6000) and sodium dodecyl sulfate (SDS) are provided by Chengdu Kelong Chemical Reagent Factory.

[0031] Example 1

[0032] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0033] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0034] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0035] 2. Weigh 500 g of the alcohol extract, add 20 g of the SDS / PEG-6000 mixture, mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation, wherein the weight ratio of SDS to PEG-6000 in the SDS / PEG-6000 mixture is 3:1.

[0036] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 1.

[0037] Example 2

[0038] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0039] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0040] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 250 mL of 50% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 50 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal liquid and the medicinal residue. The medicinal liquid was collected, poured into an evaporating dish, concentrated and dried at 100 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0041] 2. Weigh 500 g of the alcohol extract, add 20 g of the SDS / PEG-6000 mixture, mix evenly in a small V-type mixer for 35 min to obtain a Scutellaria baicalensis stem and leaf extract preparation, wherein the weight ratio of SDS to PEG-6000 in the SDS / PEG-6000 mixture is 2:1.

[0042] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 1.

[0043] Example 3

[0044] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0045] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0046] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 300 mL of 60% ethanol by volume was added for reflux alcohol extraction, reflux alcohol extraction once, and the reflux alcohol extraction time was 70 min. The extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal liquid and the medicinal residue. The medicinal liquid was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0047] 2. Weigh 500 g of the alcohol extract, add 20 g of the SDS / PEG-6000 mixture, mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation, wherein the weight ratio of SDS to PEG-6000 in the SDS / PEG-6000 mixture is 1:1.

[0048] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 1.

[0049] At the same time, a weight ratio control 1 and a weight ratio control 2 were set, that is, except for the weight ratio of SDS and PEG-6000 in the mixture, the other conditions were exactly the same as those in Example 1. The weight ratio of SDS and PEG-6000 and the dissolution of the Scutellaria baicalensis stem and leaf extract preparation are shown in Table 1. Comparative Example 1

[0050] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0051] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0052] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0053] 2. Weigh 500 g of the alcohol extract, add 20 g of SDS, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0054] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0055] Comparative Example 2

[0056] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0057] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0058] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0059] 2. Weigh 500 g of the alcohol extract, add 25 g of SDS, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0060] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0061] Comparative Example 3

[0062] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0063] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0064] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0065] 2. Weigh 500 g of the alcohol extract, add 30 g of SDS, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0066] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0067] Comparative Example 4

[0068] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0069] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0070] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0071] 2. Weigh 500 g of the alcohol extract, add 10 g of SDS, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0072] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0073] Comparative Example 5

[0074] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0075] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0076] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0077] 2. Weigh 500 g of the alcohol extract, add 15 g of SDS, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0078] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0079] Comparative Example 6

[0080] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0081] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0082] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0083] 2. Weigh 500 g of the alcohol extract, add 20 g of PEG-6000, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0084] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0085] Comparative Example 7

[0086] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0087] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0088] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0089] 2. Weigh 500 g of the alcohol extract, add 25 g of PEG-6000, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0090] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0091] Comparative Example 8

[0092] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0093] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0094] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0095] 2. Weigh 500 g of the alcohol extract, add 30 g of PEG-6000, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0096] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0097] Comparative Example 9

[0098] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0099] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0100] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0101] 2. Weigh 500 g of the alcohol extract, add 10 g of PEG-6000, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0102] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0103] Comparative Example 10

[0104] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0105] 1. Extract the stems and leaves of Scutellaria baicalensis radix with alcohol to obtain an alcohol extract, the method is as follows:

[0106] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and an alcohol extract was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0107] 2. Weigh 500 g of the alcohol extract, add 15 g of PEG-6000, and mix evenly in a small V-type mixer for 30 min to obtain a Scutellaria baicalensis stem and leaf extract preparation.

[0108] After taking out, 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and stirred until the scutellaria stem and leaf extract preparation was dissolved. At the same time, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0109] Comparative Example 11

[0110] A method for preparing a scutellaria baicalensis stem and leaf extract preparation, comprising the following steps:

[0111] The dried, crushed, and 80-mesh sieve-passed Scutellaria stem and leaf sample powder was accurately weighed to 10 g, and 200 mL of 70% ethanol by volume was added for reflux alcohol extraction. The reflux alcohol extraction was repeated twice, and each reflux alcohol extraction time was 30 min. The extracts from the two extractions were combined. The combined extract was transferred to a centrifuge tube and centrifuged at 5000 rpm / min for 10 min to separate the medicinal solution and the medicinal residue. The medicinal solution was collected, poured into an evaporating dish, concentrated and dried at 105 ° C, and the Scutellaria stem and leaf extract preparation was obtained and stored. If the demand for alcohol extract is large in the subsequent steps, the amount of Scutellaria stem and leaf sample powder and ethanol can be magnified by the same multiple.

[0112] 10 g of the scutellaria stem and leaf extract preparation was weighed into a beaker, and 2000 mL of drinking water was added, and the mixture was stirred until the scutellaria stem and leaf extract preparation was dissolved. Meanwhile, the dissolution of the scutellaria stem and leaf extract preparation was observed and recorded, and the dissolution of the scutellaria stem and leaf extract preparation is shown in Table 2.

[0113] Table 1 Effect of SDS and PEG-6000 mixture on the solubility of Scutellaria baicalensis stem and leaf extract

[0114]

[0115] In Table 1, a greater number of "+"s indicates a greater amount of sticky precipitate.

[0116] As can be seen from Table 1, when SDS and PEG-6000 are added in a mass ratio of 1 to 3:1, the Scutellaria stem and leaf extract can be completely dissolved. When the mass ratio of SDS and PEG-6000 is 3:1, the solubilizing effect on the Scutellaria stem and leaf extract is the best. At this time, the liquid is clear, without obvious undissolved impurities, and the stirring time is the shortest.

[0117] Table 2 Effects of different solvent additions on the solubility of Scutellaria baicalensis stem and leaf extract

[0118]

[0119]

[0120] In Table 2, “ / ” indicates that the item does not exist, and a greater number of “+” indicates a greater amount of sticky precipitate.

[0121] It can be seen from Example 1, Comparative Example 1 and Comparative Example 6 that when the mass of the added solubilizing agent is the same, the solubilizing effect of a single SDS or PEG-6000 is not as strong as that of a mixed solubilizing agent; it can be seen from Comparative Examples 1 to 5 or Comparative Examples 6 to 10 that as the amount of the added solubilizing agent increases, the solubilizing effect becomes stronger. Only when the amount of SDS added reaches more than 25g, or the amount of PEG-6000 added reaches more than 30g, can the Scutellaria baicalensis stem and leaf extract be completely dissolved.

[0122] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0123] The above-mentioned embodiments only express several implementation methods of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A method for preparing a preparation of a scutellaria baicalensis stem and leaf extract, characterized in that: The following steps are involved: Extracting the stems and leaves of Scutellaria baicalensis radix by alcohol to obtain an alcohol extract; The alcohol extract and the sodium dodecyl sulfate / polyethylene glycol-6000 mixture are evenly mixed to obtain a Scutellaria baicalensis stem and leaf extract preparation, wherein the weight ratio of the alcohol extract to the sodium dodecyl sulfate / polyethylene glycol-6000 mixture is 50:2-3, and the sodium dodecyl sulfate / polyethylene glycol-6000 mixture is prepared by mixing sodium dodecyl sulfate and polyethylene glycol-6000 in a weight ratio of 1-3:

1.

2. The preparation method according to claim 1, characterized in that The weight ratio of the alcohol extract to the sodium lauryl sulfate / polyethylene glycol-6000 mixture is 25:

1.

3. The preparation method according to claim 1, characterized in that: The weight ratio of the sodium lauryl sulfate to polyethylene glycol-6000 is 3:

1.

4. The preparation method according to claim 1, characterized in that: The mixing time is 30 to 35 minutes.

5. The preparation method according to claim 1, characterized in that: The preparation of the alcohol extract comprises the following steps: Weigh the dried and crushed scutellaria stem and leaf samples, add 50% to 70% ethanol by volume for reflux extraction, collect the extract, centrifuge the extract, collect the medicinal solution, concentrate and dry the medicinal solution, and cool it to room temperature to obtain an alcohol extract; The solid-liquid ratio of the scutellaria baicalensis stem and leaf sample to ethanol is 1:20-30.

6. The preparation method according to claim 5, characterized in that: The solid-liquid ratio of the scutellaria baicalensis stem and leaf sample to ethanol is 1:

20.

7. The preparation method according to claim 5, characterized in that: The number of times of reflux alcohol extraction is 1 to 2 times.

8. The preparation method according to claim 5, characterized in that: The reflux alcohol extraction time is 30 min to 70 min.

9. The preparation method according to claim 5, characterized in that: The temperature of the concentrated drying is 100°C to 105°C.

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