Tumor specimen sealing device and method

Abstract

The invention relates to the technical field of specimen storage equipment, in particular to a tumor specimen sealing device. Comprising a cold storage tank, a plurality of sealing chambers are embedded in a port of the cold storage tank, a plurality of clamping plates with a shockproof function are arranged in the sealing chambers in an annular array mode, first pumps in one-to-one correspondence with the sealing chambers are arranged on the inner bottom wall of the cold storage tank, the outlet ends of the first pumps communicate with an electromagnetic reversing valve, and outlets of the electromagnetic reversing valve communicate with a first pipe and a second pipe correspondingly; the upper end of the first pipe is inserted into the sealing chamber, the bottom end of the sealing chamber is communicated with a second pump, the upper end of the second pipe is communicated with diversion pipes corresponding to the clamping plates one to one, and the other ends of the diversion pipes surround the corresponding clamping plates and then penetrate and extend out of the bottom wall of the sealing chamber. According to the scheme, liquid nitrogen and the clamping plates are subjected to cold exchange, and the temperature is slowly reduced to the set temperature; and then the temperature is rapidly reduced to the liquid nitrogen temperature in a liquid nitrogen coating and immersing mode. The extracellular crystallization is realized, and the intracellular solution is vitrified, so that the damage of cooling to tumor tissues can be reduced, and the activity of tumor tissue cells is ensured.

Description

Technical Field

[0001] The present application relates to the technical field of specimen preservation equipment, and in particular to a tumor specimen sealing device. Background Art

[0002] Tumors are major diseases that seriously threaten human life and health. In order to reduce the morbidity and mortality of tumors, researchers from various countries are conducting unremitting research. Preserving surgically removed tumor tissues, establishing tumor tissue banks, and then studying and utilizing them will greatly promote the development of anti-tumor research. If tumor specimens are active under low-temperature preservation, and various biochemical and physical indicators are equivalent to fresh tissues, the reliability of the sample will be greatly improved. If active tumor tissues are cultured, more parallel samples can be obtained, which will greatly alleviate the problem of sample shortage; if active tumor tissue cells are implanted into experimental animals, they can be used for tumor immunotherapy. Therefore, it is of great significance to study the cryopreservation technology of tumor tissues.

[0003] Most metabolic activities in organisms are catalyzed by enzymes, and low temperatures can inhibit enzyme activity, thereby inhibiting the biochemical activities of organisms. Therefore, in theory, tumor tissue can be preserved for a long time at low temperatures. However, during the cooling process, tumor tissue is easily damaged by factors such as freezing and melting of the solution.

[0004] At present, the generally accepted reason for cell damage during cooling is the "two-factor hypothesis": one is that too fast cooling causes ice formation in cells, and ice crystals cause physical damage to cells; the other is solute damage caused by too slow cooling. Therefore, it can be known that both rapid cooling and continuous slow cooling will damage tumor tissue and affect activity, and then affect the reliability of its research.

[0005] To this end, the present application provides a tumor specimen sealing device, which can minimize the damage to the tumor tissue caused by cooling while preserving the tumor tissue specimen at low temperatures.

[0006] Application Contents The purpose of the present application is to solve the problems existing in the prior art and to propose a tumor specimen sealing device.

[0007] In order to achieve the above purpose, this application adopts the following technical solutions: A tumor specimen sealing device comprises a cold storage tank with a heat-insulating layer and a tank cover, wherein a heat-insulating sealing plate is provided at the port of the cold storage tank, a plurality of sealed chambers are embedded in the sealing plate, a plurality of splints with a shockproof function are provided in a circular array in the sealed chamber, a sealing cover is provided at the port of the sealed chamber, a pump 1 corresponding to the sealed chamber is provided on the inner bottom wall of the cold storage tank, an electromagnetic reversing valve is connected to the outlet end of the pump 1, a pipe 1 and a pipe 2 are respectively connected to the outlet end of the electromagnetic reversing valve, an upper end of the pipe 1 is inserted into the sealed chamber, a pump 2 is connected to the bottom end of the sealed chamber, a shunt pipe corresponding to the splint one to one is connected to the upper end of the pipe 2, and a shunt pipe corresponding to the splint one to one is connected to the other end of the shunt pipe after being wrapped around the corresponding splint and extending through the bottom wall of the sealed chamber.

[0008] Preferably, the cold storage tank is provided with an interlayer, a pump three communicating with the cold storage tank is provided at the lower end of the interlayer, an air pipe communicating with the sealed chamber is provided at the upper end of the interlayer, a pressure relief valve is provided in the air pipe, and the outlet of the electromagnetic reversing valve is also connected to a pipe three, which is inserted into the sealed chamber and an atomizing nozzle is installed at the port.

[0009] Preferably, a plurality of elastic support blocks are evenly distributed on the bottom wall of the sealed chamber.

[0010] Preferably, a plurality of notches are vertically spaced apart on one side of the clamping plate facing the center of the sealing chamber.

[0011] Preferably, the inner wall of the cold storage tank is embedded with a plurality of heat conducting plates inserted into the interlayer.

[0012] Preferably, a speaker cover is provided at the bottom end of the cover.

[0013] Preferably, a plurality of elastic blocks are evenly distributed between the clamping plate and the inner wall of the sealing chamber.

[0014] Preferably, the can cover is provided with openings corresponding one to one with the sealing caps.

[0015] Preferably, a filling tube extending into the bottom end of the cold storage tank is embedded in the axis of the tank cover.

[0016] The sealing method of the tumor specimen sealing device comprises the following steps: S1: precooling; S2: The specimen is put into sealing; S3: Slowly cool down to a certain temperature; S4: Rapidly cool down to liquid nitrogen temperature.

[0017] Compared with the prior art, the present application provides a tumor specimen sealing device, which has the following beneficial effects: 1. When in use, pump one first draws liquid nitrogen into tube two, and the liquid nitrogen flows along tube two into the shunt tube, and then flows into the cold storage tank from the end of the shunt tube. In this process, the low temperature of the liquid nitrogen is transmitted to the splint, and the splint transmits the low temperature to the cold storage tube, thereby slowly cooling the internal environment of the cold storage tube. When the temperature in the sealed chamber drops to the set temperature, pump one injects liquid nitrogen into tube one and enters the sealed chamber. The liquid nitrogen accumulates in the sealed chamber and covers the cold storage tube. The cold storage tube is immersed in liquid nitrogen over a large area and cooled to a temperature close to or the same as that of the liquid nitrogen, thereby achieving the purpose of rapid cooling. This scheme first uses liquid nitrogen to exchange cold with the splint, and then conducts contact cooling with the cold storage tube. The tumor tissue cells are first slowly cooled to the set temperature in a solution containing a cryoprotectant; then the liquid nitrogen is used to cover and immerse them to quickly cool them to the liquid nitrogen temperature. This process ultimately achieves partial crystallization and vitrification, that is, there are crystals outside the cells while the intracellular solution reaches vitrification, which can greatly reduce the damage to tumor tissue caused by cooling and ensure the activity of tumor tissue cells during cold storage.

[0018] 2. After the cold storage tube is immersed in liquid nitrogen over a large area, pump 2 is started and maintains the same delivery efficiency as pump 1, so that the amount of liquid nitrogen injected into the sealed chamber by pump 1 and the amount of liquid nitrogen pumped out by pump 2 remain the same, so that the liquid nitrogen continues to circulate to ensure a good cooling effect.

[0019] 3. When the cold storage tube is taken out, the second pump continues to run to completely extract the liquid nitrogen in the sealed chamber. At this time, opening the cover will reduce the amount of liquid nitrogen that is vaporized and overflowed due to contact with the outside world, reducing waste.

[0020] 4. Each cold storage tube is stored individually to avoid cross infection between tissue samples; and the entire process is carried out under sterile conditions to avoid contamination by exogenous microorganisms.

[0021] 5. Liquid nitrogen is isolated from the external environment through the sealed chamber, and the liquid nitrogen flows and is discharged outside the sealed chamber through the cooperation of tubes 1, 2 and 3. When opened, direct contact between liquid nitrogen and the external environment is avoided, thereby reducing liquid nitrogen contamination and avoiding affecting the aseptic cold storage conditions of the cold storage tube.

[0022] Other advantages, objectives and features of the present application will be described in the following description to some extent; and will be apparent to those skilled in the art based on the following examination and study to some extent; or, may be taught from the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional schematic diagram of this application.

[0024] Figure 2 This is a top view schematic diagram of the present application.

[0025] Figure 3 For this application Figure 2 Schematic diagram of the cross section at AA.

[0026] Figure 4 It is a cross-sectional view, a top perspective view, and a bottom perspective view of the can cover of the present application.

[0027] Figure 5 For this application Figure 3 Schematic diagram of the cross-sectional view of the assembly inside the cold storage tank.

[0028] Figure 6 This is a schematic diagram of the splint structure of this application.

[0029] Figure 7 For this application Figure 5 Partial schematic diagram of point B.

[0030] Figure 8 This is another schematic diagram of the installation structure of the positions of pump one and pump two in the cold storage tank of the present application.

[0031] Fig. 9 This is a schematic diagram of the air pressure balance regulating device in the sealed chamber of the present application.

[0032] Fig.10 For this application Fig. 9 Partial schematic diagram at point C.

[0033] In the figure: 1. cold storage tank; 2. sealing plate; 3. sealing chamber; 4. tank cover; 5. sealing cover; 6. splint; 7. elastic block; 8. elastic support block; 9. interlayer; 10. pump one; 11. pipe one; 12. pipe two; 13. pipe three; 14. pump two; 15. pump three; 16. air pipe; 17. horn cover; 18. atomizing nozzle; 19. diverter pipe; 20. solenoid reversing valve; 21. heat transfer plate; 22. stuffing tube; 23. compartment; 24. pressure regulating pipe; 25. cylinder; 26. pressure spring; 27. pressure claw; 28. buckle claw; 29. ​​ejector rod; 30. pressure regulating channel; 31. sealing ball; 32. nitrogen spring; 33. vacuum pump; 34. ejector block. DETAILED DESCRIPTION

[0034] The following will be combined with the attached examples of the present application Figure 1-10 , the technical solutions in the embodiments of the present application are described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments.

[0035] Embodiment 1, in order to solve the problem of possible damage to tumor tissue during low-temperature storage and cooling in the prior art, this embodiment provides a tumor specimen sealing device, including a cold storage tank 1 with a heat insulation layer and a tank cover 4, wherein a heat insulation sealing plate 2 is provided at the port of the cold storage tank 1, a plurality of sealed chambers 3 are embedded on the sealing plate 2, a plurality of splints 6 with a shockproof function are provided in a circular array in the sealed chamber 3, a sealing cover 5 is provided at the port of the sealed chamber 3, a pump 10 corresponding to the sealed chamber 3 is provided on the bottom wall of the cold storage tank 1, an electromagnetic reversing valve 20 is connected to the outlet end of the pump 10, and a pipe 11 and a pipe 2 are connected to the outlet of the electromagnetic reversing valve 20 respectively, the upper end of the pipe 11 is inserted into the sealed chamber 3, the bottom end of the sealed chamber 3 is connected to the pump 2 14, the upper end of the pipe 2 12 is connected to a shunt pipe 19 corresponding to the splint 6 one by one, and the other end of the shunt pipe 19 is surrounded by the corresponding splint 6 and then extends out from the bottom wall of the sealed chamber 3.

[0036] Principle details of this embodiment: A tumor specimen sealing device comprises a cold storage tank 1 and a tank cover 4. The cold storage tank 1 and the tank cover 4 are flange structures and are fastened with self-locking bolts and nuts, so that the cold storage tank 1 and the tank cover 4 can be separated, which is convenient for internal maintenance and replacement. Sterile liquid nitrogen is injected into the cold storage tank 1.

[0037] The cold storage tank 1 is embedded with a sealing plate 2 at the port, and the sealing plate 2 has a plurality of through holes in a circular array on the side thereof. The cold storage tank 1 port is provided with threaded holes corresponding to the through holes one by one, and the screw is passed through the through hole and screwed into the threaded hole, so as to realize the fastening installation of the sealing plate 2 at the port of the cold storage tank 1. A sealing strip is provided at the contact part between the sealing plate 2 and the port of the cold storage tank 1 to increase the airtightness and prevent the leakage of cold air. There are a plurality of mounting holes in a circular array on the sealing plate 2, and a plurality of sealing chambers 3 are fastened and installed in the mounting holes. A sealing cover 5 is provided at the port of the sealing chamber 3, and the sealing cover 5 and the periphery of the sealing chamber 3 are also flange structures and are also fastened and connected by screws. A sealing gasket is provided at the contact part between the sealing cover 5 and the sealing chamber 3 to prevent air leakage. A plurality of splints 6 are provided in a circular array in the sealing chamber 3, and the splint 6 has a shockproof function, and is used to clamp storage containers such as sample tubes containing tumor tissue, and has a shockproof effect. The splint 6 has good heat and cold transfer properties, such as copper 401, aluminum 237, iron 80, and tin 67. A pump 10 corresponding to the sealed chamber 3 is provided on the inner bottom wall of the cold storage tank 1, and the pump 10 has an inlet end and an outlet end. The inlet end of the pump 10 is close to the bottom wall of the cold storage tank 1, and is used to suck liquid nitrogen. The outlet end of the pump 10 is connected to an electromagnetic reversing valve 20. In this embodiment, the electromagnetic reversing valve 20 is a two-way valve, having an inlet and two non-interconnected outlets. The two outlets of the electromagnetic reversing valve 20 are respectively connected to a pipe 11 and a pipe 2 12. The upper end of the pipe 11 is inserted into the sealed chamber 3, and the bottom end of the sealed chamber 3 is connected to a pump 2 14 located in the cold storage tank 1, and the outlet end of the pump 2 14 extends into the cold storage tank 1. The upper end of the pipe 2 12 is connected to a shunt pipe 19 corresponding to the splint 6. After the other end of the shunt pipe 19 is inserted from the bottom end of the corresponding splint 6, it is serpentine and twisted in the splint 6, and then extends from the other side of the bottom end of the splint 6 and extends through the bottom wall of the sealed chamber 3.

[0038] In this embodiment, low-temperature resistant temperature sensors, such as Cernox® temperature sensors, are provided in the cold storage tank 1 and the sealed chamber 3, which can accurately measure the ambient temperature in a low-temperature environment and monitor the storage environment temperature conditions of the specimens in real time.

[0039] In this embodiment, the cold storage tank 1, the tank cover 4, the sealing plate 2, the sealing chamber 3, and the sealing cover 5 are all provided with a heat-insulating layer to prevent cold air leakage and ensure the stability of the cold storage environment of the tumor tissue.

[0040] In this embodiment, in order to prevent the normal operation of the pump 10 and the pump 2 14 from being affected by the low temperature in the liquid nitrogen, two structural forms are provided in this embodiment to isolate the low temperature: The first type: the exterior of multiple pumps 10 and pumps 2 14 are all irradiated with a thermal insulation cover, and a heat insulation layer is arranged inside the thermal insulation cover. The inlet and outlet of pump 1 10 and pump 2 14 respectively pass through the thermal insulation cover, thereby playing a certain anti-low-temperature freezing function.

[0041] The second type: refer to the attached Figure 8As shown, a compartment 23 is provided at the bottom of the cold storage tank 1, and a heat insulation layer is provided in the compartment 23. Pump 1 10 and pump 2 14 are located in the compartment 23, thereby being isolated from liquid nitrogen, thereby playing a certain anti-low-temperature freezing function.

[0042] In this embodiment, the entire process and each instrument are sterilized; and when not in use, they are sterilized regularly.

[0043] According to the above technical solution: When in use, the collected specimens are first fixed in an appropriate fixative to fix the structure and morphology of tissue cells. The fixed tissue specimens are then buried in a wax block. The buried specimens are then cut into thin slices. The specimen holder is then placed in a cold storage tube containing a cryoprotectant and the lid is quickly screwed on (before using the cold storage tube, first unscrew the cold storage tube lid, clamp the upper and middle section of the cold storage tube with tweezers, and place it in a liquid nitrogen cup for precooling). The cold storage tube is then placed in a sealed chamber 3 and clamped by a clamp 6. A cover 5 is added.

[0044] The pump 10 corresponding to the sealed chamber 3 is started, and the pump 10 first draws liquid nitrogen into the tube 2 12. The liquid nitrogen enters the shunt tube 19 along the tube 2 12, and then flows into the cold storage tank 1 from the end of the shunt tube 19. In this process, the low temperature of the liquid nitrogen is transmitted to the splint 6, and the splint 6 with good cooling performance then transmits the low temperature to the cold storage tube, thereby slowly cooling the internal environment of the cold storage tube. When the temperature sensor in the sealed chamber 3 detects that the cooling tube has cooled to the set temperature, the electromagnetic reversing valve 20 is reversed. At this time, the pump 10 injects liquid nitrogen into the tube 11 and enters the sealed chamber 3. The liquid nitrogen accumulates in the sealed chamber 3 and covers the cold storage tube. The cold storage tube is immersed in a large area of ​​liquid nitrogen and the temperature is reduced to a temperature close to or the same as that of the liquid nitrogen, thereby achieving the purpose of rapid cooling. After the cold storage tube is immersed in liquid nitrogen over a large area, pump 2 14 is started and maintains the same delivery efficiency as pump 1 10, so that the amount of liquid nitrogen injected into the sealed chamber 3 by pump 10 and the amount of liquid nitrogen extracted by pump 2 14 remain the same, so that the liquid nitrogen continues to circulate to ensure a good cooling effect.

[0045] When the cold storage tube is taken out, the electromagnetic reversing valve 20 makes the pump 10 communicate with the pipe 2 12, circulates the liquid nitrogen in the shunt pipe 19, and the clamping plate 6 contacts the cold storage tube to continue refrigeration; while the pump 2 14 continues to operate to completely extract the liquid nitrogen in the sealed chamber 3. At this time, the cover 5 is opened again to take out the cold storage tube, which will reduce the amount of liquid nitrogen gasified and overflowed due to contact with the outside world, thereby reducing waste.

[0046] Each cryotube is stored individually to avoid cross infection between tissue samples; and the entire process is carried out under sterile conditions to avoid contamination by exogenous microorganisms.

[0047] The liquid nitrogen is isolated from the external environment by the sealed chamber 3, and the liquid nitrogen flows and is discharged outside through the cooperation of the tube 1 11, the tube 2 12, and the tube 3 13. When opened, the direct contact between the liquid nitrogen and the external environment is avoided, thereby reducing the liquid nitrogen contamination and avoiding the impact on the aseptic cold storage conditions of the cold storage tube.

[0048] In summary, this solution first uses liquid nitrogen to exchange cold with the splint 6, and then conducts contact cooling with the cold storage tube, first slowly cooling the tumor tissue cells in a solution containing a cryoprotectant to a set temperature; and then quickly cooling them to liquid nitrogen temperature by covering and immersing them in liquid nitrogen. This process ultimately achieves partial crystallization and vitrification, that is, there are crystals outside the cells and the intracellular solution reaches vitrification, which can reduce the damage to the tumor tissue by cooling to a greater extent, and ensure the activity of tumor tissue cells in cold storage.

[0049] Embodiment 2, in a further embodiment of this scheme, due to the different cell structures, sizes and properties, different cells require different optimal cooling rates. Although in the slow cooling stage, the liquid nitrogen delivery efficiency can be adjusted by adjusting the operating power of pump 10 and pump 2 14, and then the cooling efficiency can be adjusted, but this method of controlling the liquid nitrogen flow rate is too slow and inefficient. Therefore, in this embodiment: The cold storage tank 1 is provided with an interlayer 9, a pump three 15 communicating with the cold storage tank 1 is provided at the lower end of the interlayer 9, an air pipe 16 communicating with the sealed chamber 3 is provided at the upper end of the interlayer 9, a pressure relief valve is provided in the air pipe 16, the outlet of the electromagnetic reversing valve 20 is also connected with a pipe three 13, the pipe three 13 is inserted into the sealed chamber 3 and an atomizing nozzle 18 is installed at the port.

[0050] Principle details of this embodiment: In this embodiment, the electromagnetic reversing valve 20 is a three-way valve, that is, one inlet and three outlets. The inlet of the electromagnetic reversing valve 20 is connected to the outlet end of the pump 10, and the three outlets of the electromagnetic reversing valve 20 are respectively connected to the pipe 11, the pipe 2 12, and the pipe 3 13. The pipe 11 is directly inserted into the sealed chamber 3, the pipe 2 12 is connected to the shunt pipe 19, and the pipe 3 13 is also inserted into the sealed chamber 3 and the end of the pipe 3 13 is installed with an atomizing nozzle 18, and the atomizing nozzle 18 is a liquid nitrogen atomizer for atomizing liquid nitrogen. The side wall of the sealed chamber 3 is inserted with an air pipe 16, and the air pipe 16 is provided with a pressure relief valve. The side wall of the cold storage tank 1 is provided with an interlayer 9, and the interlayer 9 is also provided with a heat insulation layer to avoid cold leakage. The end of the air pipe 16 is inserted into the interlayer 9. The bottom end of the interlayer 9 is provided with a pump 3 15, and the pump 3 15 is also provided with a temperature insulation cover. The inlet port of pump three 15 is located at the bottom end of interlayer 9, and the outlet end is inserted into cold storage tank 1.

[0051] In this embodiment, the pressure relief valve is an electric valve, and an air pressure sensor is provided in the sealed chamber 3, and the air pressure sensor is electrically connected to the electric valve. When the air pressure in the sealed chamber 3 reaches a set value, the electric valve opens to release the pressure.

[0052] According to the above technical solution: When in use, the electromagnetic reversing valve 20 is the same as the pipe 2 12 and the pipe 3 13 at the same time, so that the liquid nitrogen circulates in the shunt pipe 19 on one side and exchanges heat with the cold storage pipe through the splint 6; the liquid nitrogen in the pipe 3 13 at the other end enters the sealed chamber 3 after being atomized by the atomizing nozzle 18, and the gaseous nitrogen contacts the cold storage pipe, expanding the contact range of the cold storage pipe with the low-temperature nitrogen, thereby improving the cooling efficiency. By adjusting the atomization efficiency, the cooling efficiency can be controlled, and the control range is wider. The gaseous nitrogen content in the sealed chamber 3 gradually increases. In order to avoid safety hazards caused by continuous pressurization, when the air pressure in the sealed chamber 3 reaches a certain level, the pressure relief valve automatically opens to introduce the gaseous nitrogen into the interlayer 9 to achieve the purpose of pressure relief. The gaseous nitrogen accumulates in the interlayer 9 and is cooled and liquefied. The re-liquefied liquid nitrogen will be pumped back to the cold storage tank 1 by the pump 3 15 for continued use, saving resources and avoiding waste.

[0053] Embodiment 3, in a further embodiment of the present invention, a plurality of elastic support blocks 8 are evenly distributed on the inner bottom wall of the sealed chamber 3. The elastic support blocks 8 support the bottom end of the cold storage tube, thereby preventing the cold storage tube from shaking and contacting the wall and being damaged, resulting in leakage and contamination of the tissue; and the bottom end of the cold storage tube can be fully contacted by the gas phase nitrogen to ensure uniform cooling efficiency.

[0054] Embodiment 4, in a further embodiment of the present invention, the clamping plate 6 is provided with a plurality of notches at intervals vertically on one side facing the center of the sealing chamber 3. Through the plurality of notches, the gaseous nitrogen can contact the cold storage tube when the clamping is unstable, thereby increasing the contact area between the gaseous nitrogen and the cold storage tube, thereby ensuring the uniform cooling of the cold storage tube to the greatest extent possible.

[0055] Embodiment 5, in a further embodiment of the present scheme, in order to avoid excessive gas pressure due to excessive gaseous nitrogen content injected into the interlayer 9, which may easily cause the tank wall of the cold storage tank 1 to crack. In this embodiment, the inner wall of the cold storage tank 1 is embedded with a plurality of heat conducting plates 21 inserted into the interlayer 9. The heat conducting plates 21 are made of cold conducting materials, such as copper 401, aluminum 237, iron 80, and tin 67. One end of the heat conducting plate 21 is inserted into the cold storage tank 1, and the other end extends into the interlayer 9. The liquid nitrogen in the cold storage tank 1 is contacted and heat-exchanged with the gaseous nitrogen in the interlayer 9 through the heat conducting plate 21, which accelerates the aggregation of the gaseous nitrogen and condenses it into liquid nitrogen.

[0056] Embodiment 6, in a further embodiment of the present invention, in order to prevent the mouth of the cold storage tube from being immersed in liquid nitrogen, resulting in a large amount of liquid nitrogen in the tube rapidly heating up and causing explosion during resuscitation, in this embodiment, a trumpet cover 17 is provided at the bottom of the cover 5. The diameter of the trumpet cover 17 is larger than the diameter of the space formed by the buckling of multiple clamps 6, so as to ensure that the cold storage tube has enough space for placement and avoid interference between the trumpet cover 17 and the upper end of the cold storage tube.

[0057] After the cover 5 is buckled in this way, the trumpet cover 17 is buckled on the upper part of the cold storage tube. Liquid nitrogen is injected into the sealed chamber 3 through the tube 11 until the surface of the liquid nitrogen submerges the bottom end of the trumpet cover 17. At this time, the gas is concentrated and controlled in the trumpet cover 17, and the top of the cold storage tube is in the trumpet cover 17. The gas in the sealed chamber 3 is squeezed and gathered at the top. Under the action of air pressure, the liquid nitrogen can never rise to the top of the sealed chamber 3, thereby exposing the upper end of the cold storage tube to the outside of the liquid nitrogen, thereby avoiding the tube mouth of the cold storage tube from being immersed in liquid nitrogen, resulting in a large amount of liquid nitrogen in the tube rapidly heating up and causing the problem of bursting during resuscitation. (When the air pipe 16 exists, the bottom end of the trumpet cover 17 is located below the air pipe 16 to reduce the amount of gas overflowing from the air pipe 16 when the air pressure in the sealed chamber 3 increases, so as to ensure that there is enough air pressure in the trumpet cover 17 to depress the liquid nitrogen water level and leave space for the cold storage tube port.

[0058] Preferably, the upper end of the horn cover 17 is closed and presents a cylindrical hole shape, and this closed part is buckled on the upper end of the cold storage tube, thereby isolating the upper end of the cold storage tube from the inside of the sealing chamber 3, thereby increasing the tube end isolation property of the cold storage tube.

[0059] Embodiment 7, in a further embodiment of the present solution, a specific structure is provided that enables the clamping plate 6 to realize the shockproof function: a plurality of elastic blocks 7 are evenly distributed between the clamping plate 6 and the inner wall of the sealing chamber 3. The elastic blocks 7 are made of low-temperature resistant materials, such as ultra-low temperature (77K) elastic organic crystal materials. The elastic blocks 7 are used to abut against the clamping plate 6, so that the clamping plate 6 can not only clamp the cold storage tube, but also maintain a certain elastic range to achieve the purpose of shockproof.

[0060] Embodiment 8, in the further embodiment of this scheme, refer to the attached Figure 4 As shown, under the premise of setting sufficient heat preservation and heat insulation conditions, the tank cover 4 is provided with a notch corresponding to the sealing cover 5. In this way, when the sealed chamber 3 needs to be opened, there is no need to open the tank cover 4, only the sealing cover 5 needs to be opened, making the whole process more convenient and efficient.

[0061] Embodiment 9, in a further embodiment of the present invention, the tank cover 4 is axially embedded with a stuffing tube 22 extending into the bottom end of the cold storage tank 1. The end of the stuffing tube 22 is provided with a heat-insulating sealing plug. The sealing plug completely covers the upper end of the stuffing tube 22 to prevent the cold from being conducted and affecting the temperature balance in the cold storage tank 1.

[0062] When in use, liquid nitrogen can be filled or pumped out through the filling tube 22 for replenishment of liquid nitrogen and external disinfection and sterilization of liquid nitrogen, making the use of the device more convenient and practical.

[0063] The entire operating principle of the equipment in Examples 1-9: After placing the cold storage tube in the sealed chamber 3, start the pump 10 corresponding to the sealed chamber 3, and the pump 10 first draws liquid nitrogen into the tube 2 12 and the tube 3 13: The liquid nitrogen in the second pipe 12 flows into the shunt pipe 19, and then flows into the cold storage tank 1 from the end of the shunt pipe 19. In this process, the low temperature of the liquid nitrogen is transferred to the clamping plate 6, and the clamping plate 6 with good cooling performance transfers the low temperature to the cold storage pipe, thereby achieving the purpose of slow cooling.

[0064] The liquid nitrogen in the tube 3 13 is atomized by the atomizing nozzle 18 and enters the sealed chamber 3. The gaseous nitrogen contacts the cold storage tube, expanding the contact range of the cold storage tube with the low-temperature nitrogen, thereby improving the cooling efficiency. By adjusting the atomization efficiency, the slow cooling efficiency can be controlled, which is suitable for tumor tissue cells of different structures, sizes and properties.

[0065] The gaseous nitrogen content in the sealed chamber 3 gradually increases. In order to avoid potential safety hazards caused by continuous pressurization, when the air pressure in the sealed chamber 3 reaches a certain level, the pressure relief valve automatically opens to introduce the gaseous nitrogen into the interlayer 9 to achieve the purpose of pressure relief. The gaseous nitrogen accumulates in the interlayer 9 and is cooled and liquefied. The re-liquefied liquid nitrogen will be pumped back to the cold storage tank 1 by the pump 3 15 for continued use.

[0066] When the temperature sensor in the sealed chamber 3 detects that the cooling pipe has cooled to the set temperature, the electromagnetic reversing valve 20 is reversed, and at this time, the pump 10 injects liquid nitrogen into the tube 11 and enters the sealed chamber 3. The liquid nitrogen accumulates in the sealed chamber 3 and covers the cold storage tube. Under the action of the horn cover 17, the gas in the sealed chamber 3 is squeezed and gathered at the top. Under the action of the air pressure, the liquid nitrogen can never rise to the top of the sealed chamber 3, thereby exposing the upper end of the cold storage tube to the liquid nitrogen, thereby avoiding the cold storage tube mouth being immersed in liquid nitrogen, resulting in a large amount of liquid nitrogen in the tube rapidly heating up and causing the problem of bursting during resuscitation. The cold storage tube is immersed in a large area of ​​liquid nitrogen, and the temperature is quickly reduced to a temperature close to or the same as that of the liquid nitrogen, thereby achieving the purpose of rapid cooling.

[0067] After the cold storage tube is immersed in liquid nitrogen over a large area, pump 2 14 is started and maintains the same delivery efficiency as pump 1 10, so that the amount of liquid nitrogen injected into the sealed chamber 3 by pump 10 and the amount of liquid nitrogen extracted by pump 2 14 remain the same, so that the liquid nitrogen continues to circulate to ensure a good cooling effect.

[0068] Example 10, a sealing method using a tumor specimen sealing device, comprising the following steps: S1: precooling; S2: The specimen is put into sealing; S3: Slowly cool down to a certain temperature; S4: Rapidly cool down to liquid nitrogen temperature.

[0069] Principle details of this embodiment: S1: The cold storage tube is pre-cooled to achieve the cooling speed required for vitrification; the scaffold carrying the tumor tissue system is immersed in liquid nitrogen for more than five minutes to allow the tissue to be fully and evenly cooled in liquid nitrogen.

[0070] S2: Specimen processing (fixation, embedding, slicing), and then putting the specimen into a pre-cooled cold storage tube, and then placing the cold storage tube into the sealed chamber 3 and tightening the cover 5 of the sealed chamber 3.

[0071] S3: Pump 1 10 is started to pump liquid nitrogen into pipe 2 12, and the liquid nitrogen in pipe 2 12 flows along the shunt pipe 19, and contacts the cold storage pipe through the clamp plate 6 to cool down; or liquid nitrogen enters pipe 2 12 and pipe 3 13 at the same time, and the liquid nitrogen in pipe 2 12 flows along the shunt pipe 19, and contacts the cold storage pipe through the clamp plate 6 to cool down, and the liquid nitrogen in pipe 3 13 is atomized through the atomizing nozzle 18, so that the cold storage pipe cools down more evenly. In the above manner, the cold storage pipe is cooled slowly until a certain temperature.

[0072] S4: Pump 10 is used to inject liquid nitrogen into tube 11 through electromagnetic reversing valve 20. Liquid nitrogen flows into sealed chamber 3 along tube 11 and soaks the cold storage tube to achieve rapid cooling to liquid nitrogen temperature.

[0073] In this solution, when placing the cooling tube, it is necessary to open the cover 5 of the sealed chamber 3, and air is then poured into the sealed chamber 3. After the cold storage tube is placed in the sealed chamber 3, the air pressure in the sealed chamber 3 is atmospheric pressure. In the slow cooling stage, the temperature in the sealed chamber 3 is still higher than the temperature of liquid nitrogen. At this time, the liquid nitrogen has the problem of gasification and diffusion in the sealed chamber 3. The liquid nitrogen gasifies and mixes with the air, which not only causes liquid nitrogen pollution, but also the gasified liquid nitrogen will adhere to the vicinity of the tube mouth of the cold storage tube, and adhere to the tube mouth of the cold storage tube with the subsequent rapid cooling, which is easy to cause a large amount of liquid nitrogen in the tube to quickly heat up and cause bursting in the recovery stage.

[0074] Therefore, in this solution, a pressure balance regulating device is also provided inside the sealed chamber 3, including: A pressure regulating channel 30 is arranged at the axis of the cover 5. A conical closing is arranged in the pressure regulating channel 30, and a sealing ball 31 is abutted at the conical closing. A nitrogen spring 32 is arranged below the sealing ball 31 in the pressure regulating channel 30, and the extended end of the nitrogen spring 32 abuts against the bottom end of the sealing ball 31.

[0075] A mounting plate is provided at the notch of the tank cover 4, and a cylinder 25 is fixed on the mounting plate. The cylinder 25 stands upside down on the mounting plate, and the extended end of the cylinder 25 penetrates the mounting plate. A collar is provided at the extended end of the cylinder 25, and a pressure regulating pipe 24 is fixed inside the collar. The pressure regulating pipe 24 is connected to a pump 4, and the port of the pump 4 is connected to a gas tank through a hose. The gas tank stores sterile gas, such as nitrogen, argon, and other gases suitable for storing articles. A push rod 29 is provided at the lower end of the pressure regulating pipe 24.

[0076] A vacuum pump 33 is arranged on the sealing cover 5 , and an air suction port of the vacuum pump 33 passes through the sealing cover 5 , and an air outlet is communicated with the outside.

[0077] After the cover 5 is tightly closed, the vacuum pump 33 is started first to discharge the air in the sealed chamber 3. Then the cylinder 25 is started, and the extended end of the cylinder 25 drives the pressure regulating tube 24 to descend and insert into the pressure regulating channel 30. A sealing ring is provided between the contact part between the pressure regulating tube 24 and the inner wall of the pressure regulating channel 30 to prevent leakage of the pressure regulating channel 30. After the pressure regulating tube 24 is inserted into the pressure regulating channel, the sealing ball 31 under the push rod 29 descends, so that the lower port of the pressure regulating channel 30 is connected with the pressure regulating tube 24. Then the pump 4 is started to inject the gas in the gas tank into the sealed chamber 3, thereby adjusting the air pressure in the sealed chamber 3 and exceeding the atmospheric pressure. In this way, the contaminated gas in the sealed chamber 3 is discharged and sterile gas is injected. That is, it avoids the mixing of liquid nitrogen and air and affects the aseptic storage conditions; it can also increase the pressure in the sealed chamber 3. Under normal pressure, liquid nitrogen will gasify when the temperature is lower than that, and when the pressure increases, the temperature requirement for its gasification will also increase, thereby ensuring that the liquid nitrogen entering the sealed chamber 3 remains in a liquid flow state and will not be easily gasified. After the pressure regulation is completed, the cylinder 25 contracts and drives the pressure regulating tube 24 to rise, and the sealing ball 31 rises under the action of the nitrogen spring 32 and rests on the closing part, and the pressure regulating channel 3 is closed.

[0078] Preferably, the lower end of the collar is abutted with a downward pressure spring 26, and a pressure claw 27 is installed at the bottom end of the downward pressure spring 26. The pressure claw 27 is in a four-claw inverted shape. A buckle claw 28 is provided at the upper end of the cover 5 around the pressure regulating channel 30, and the buckle claw 28 is provided with a plurality of legs against the surface of the cover 5. When the extended end of the cylinder 25 descends, the pressure claw 27 first contacts the buckle claw 28, and when the pressure regulating tube 24 is inserted into the pressure regulating channel 30, the pressure claw 27 is against the buckle claw 28 and imprisoned on the cover 5, and the downward pressure spring 26 is compressed, thereby sealing and tightening the cover 5. After the pressure regulation is completed, the extended end of the cylinder 25 is retracted to the push rod 29 and the sealing ball 31 are separated. At this time, the downward pressure spring 26 is still in a compressed state, and the cover 5 is still sealed by the pressure claw 27 and the buckle claw 28. The cylinder 25 is electrically connected to the air pressure sensor in the sealing chamber 3 and the main controller. In this way, the cover 5 is blocked by the cylinder 25 to ensure that when the cold storage tube is to be taken out, it will continue to rise only after the liquid nitrogen in the sealing chamber 3 is completely extracted, so that the pressing claw 27 and the buckle claw 28 are separated. Only then can the fastener be unlocked and the cover 5 be opened, thereby avoiding opening the cover 5 before the liquid nitrogen is completely extracted, resulting in the waste of liquid nitrogen gasification and spillage.

[0079] Preferably, the pressure regulating channel 30, the sealing ball 31 and the pressure regulating tube 24 are all covered with a thermal insulation layer to prevent cold air from leaking.

[0080] In this solution, solenoid valves are provided between all pumps and corresponding pipelines to ensure that the pipelines are in a closed state when the pumps are turned off.

[0081] The above is only a preferred specific implementation manner of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can make equivalent substitutions or changes within the technical scope disclosed in the present application according to the technical solution and application concept of the present application, which should be covered by the protection scope of the present application.

[0082] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0083] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A tumor specimen sealing device, characterized in that: The invention comprises a cold storage tank (1) having a heat insulation layer and a tank cover (4). The cold storage tank (1) is provided with a heat insulation sealing plate (2) at the port thereof. A plurality of sealed chambers (3) are embedded on the sealing plate (2). A plurality of clamping plates (6) having a shockproof function are arranged in an annular array in the sealed chambers (3). A sealing cover (5) is provided at the port of the sealed chambers (3). A pump (10) corresponding to the sealed chambers (3) is arranged on the bottom wall of the cold storage tank (1). The outlet end of the pump (10) is connected to an electromagnetic reversing valve (20). The outlet of the electromagnetic reversing valve (20) is respectively connected to a pipe (11) and a pipe (12). The upper end of the pipe (11) is inserted into the sealed chamber (3). The bottom end of the sealed chamber (3) is connected to a pump (14). The upper end of the pipe (12) is connected to a shunt pipe (19) corresponding to the clamping plates (6). The other end of the shunt pipe (19) is extended from the corresponding clamping plate (6) and then extends through the bottom wall of the sealed chamber (3).

2. A tumor specimen sealing device according to claim 1, characterized in that: The cold storage tank (1) is provided with an interlayer (9), a pump three (15) communicating with the cold storage tank (1) is provided at the lower end of the interlayer (9), an air pipe (16) communicating with the sealed chamber (3) is provided at the upper end of the interlayer (9), a pressure relief valve is provided in the air pipe (16), the outlet of the electromagnetic reversing valve (20) is also connected with a pipe three (13), the pipe three (13) is inserted into the sealed chamber (3) and an atomizing nozzle (18) is installed at the end thereof.

3. A tumor specimen sealing device according to claim 2, characterized in that: A plurality of elastic support blocks (8) are evenly distributed on the inner bottom wall of the sealed chamber (3).

4. A tumor specimen sealing device according to claim 2, characterized in that: A plurality of notches are vertically spaced apart on one side of the clamping plate (6) facing the center of the sealing chamber (3).

5. The tumor specimen sealing device according to claim 2, characterized in that: The inner wall of the cold storage tank (1) is embedded with a plurality of heat conducting plates (21) inserted into the interlayer (9).

6. The tumor specimen sealing device according to claim 1, characterized in that: A speaker cover (17) is provided at the bottom end of the sealing cover (5).

7. The tumor specimen sealing device according to claim 1, characterized in that: A plurality of elastic blocks (7) are evenly distributed between the clamping plate (6) and the inner wall of the sealing chamber (3).

8. The tumor specimen sealing device according to claim 1, characterized in that: The can cover (4) is provided with openings corresponding one to one with the sealing covers (5).

9. The tumor specimen sealing device according to claim 1, characterized in that: The tank cover (4) is axially embedded with a stuffing tube (22) extending into the bottom end of the cold storage tank (1).

10. A sealing method using the tumor specimen sealing device according to any one of claims 1 to 9, comprising the following steps: S1: precooling; S2: The specimen is put into sealing; S3: Slowly cool down to a certain temperature; S4: Rapidly cool down to liquid nitrogen temperature.

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