Composition containing 10-hydroxy-2-decenoic acid and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition containing 10-hydroxy-2-decenoic acid and application of the composition. The composition disclosed by the invention is prepared by compounding probiotic fermented brown algae, pterostilbene and 10-hydroxy-2-decenoic acid according to a certain proportion. Tests prove that all the components of the composition can generate a synergistic effect, and the composition has the effects of remarkably inhibiting generation of inflammatory factors, resisting inflammatory aging, inhibiting lipid peroxidation and the like. Meanwhile, the invention accidentally discovers that the illumination and thermal stability of the pterostilbene can be obviously improved by compounding the probiotic fermented brown algae, the 10-hydroxy-2-decenoic acid and the pterostilbene, so that the pterostilbene has good practical application value.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a composition containing 10-hydroxy-2-decenoic acid and application thereof. Background Art

[0002] The information disclosed in the background of the invention is only intended to enhance the understanding of the overall background of the invention and should not be necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Franceschi first proposed the concept of "inflammatory aging" in 2000. Inflammatory stimuli mainly come from endogenous, ectopic or denatured molecules produced by damaged and / or dead cells and organelles (cell fragments). These molecules are recognized by the receptors of the innate immune system and induce the body to produce an inflammatory response. The body produces these molecules under normal physiological conditions, which gradually increase with age, and the proteasome's autophagy and / or mitochondrial autophagy clearance gradually decreases. This "autoimmune response" process promotes the occurrence or development of chronic diseases, thereby accelerating and aggravating the local and systemic aging process. Immunogenicity refers to the decline of adaptive immune response with age, and the dominance of natural immune response. Its notable characteristics are the increase of proinflammatory mediators and the increase of inflammatory factors in serum, such as interleukin (IL) 6, IL-8, and tumor necrosis factor-α (TNF-α). A large number of studies have shown that immunoaging is a triggering factor of inflammatory aging and is believed to have a common inflammatory basis with most aging-related diseases.

[0004] Therefore, inflammation has also been identified as a major target for anti-aging strategies. As the largest organ and outermost protective barrier of the human body, the skin most directly shows the manifestations of aging, such as thinning, dryness, pigmentation and fine lines. The manifestation of the above aging manifestations is accompanied by specific epigenetic modifications, reduced cell proliferation / tissue renewal, accumulation of senescent cells, changes in the extracellular matrix, and a pro-inflammatory environment that is conducive to disease or aging.

[0005] Lipid peroxidation refers to the oxidation and deterioration of polyunsaturated fatty acids to generate lipid peroxidation products. Free radicals attack unsaturated fatty acids in the sebum membrane, destroy the components of the sebum membrane, damage the skin barrier function, and reduce the ability to lock in moisture and moisturize, thus leading to dry skin. Lipid peroxidation can destroy the integrity of the membrane and cause the membrane to lose its filtration and barrier functions, affecting the cell structure and function. In addition, the process of lipid peroxidation can trigger the occurrence of skin inflammation, seriously affecting the health and beauty of the skin. Its mechanism is that membrane lipids can be converted into autotriene (LT) and prostaglandin PG2 with strong pharmacological effects under the action of lipoxygenase and reactive oxygen, and MDA can be generated under endoperoxidation. Both metabolic pathways can produce oxygen free radicals, thus forming a circulation loop from reactive oxygen to fatty acid oxidation and then regeneration of reactive oxygen free radicals, which can amplify the inflammatory response step by step, thereby further accelerating skin aging.

[0006] Royal jelly acid, also known as 10-hydroxy-2-decenoic acid (10-HDA), is an unsaturated fatty acid in royal jelly. As a substance with multiple physiological activities, royal jelly acid has a very broad prospect for exploration and application in the direction of cosmetic development and medical application. Current research results show that royal jelly acid has anti-aging, anti-inflammatory, whitening, and antioxidant effects. However, royal jelly acid itself is relatively expensive, which greatly limits its use. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention provides a composition containing 10-hydroxy-2-decenoic acid and its application. Specifically, the present invention is a composition obtained by compounding probiotic fermented brown algae, pterostilbene and 10-hydroxy-2-decenoic acid in a certain proportion. Experiments have shown that the components of the above-mentioned composition can produce a synergistic effect, and have the effects of significantly inhibiting the production of inflammatory factors, resisting inflammatory aging, and inhibiting lipid peroxidation. At the same time, the present invention unexpectedly discovered that compounding probiotic fermented brown algae, 10-hydroxy-2-decenoic acid and pterostilbene can also

[0008] The light and thermal stability of pterostilbene can be significantly improved. Based on the above research results, the present invention is completed.

[0009] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0010] The first aspect of the present invention provides a composition containing 10-hydroxy-2-decenoic acid, wherein the composition comprises the following components in parts by weight:

[0011] 0.1-50 parts of probiotic fermented brown algae, 0.001-0.5250 parts of pterostilbene, and 0.001-2 parts of 10-hydroxy-2-decenoic acid.

[0012] The second aspect of the present invention provides use of the above composition in preparing cosmetics.

[0013] The cosmetic has at least one or more of the following functions:

[0014] (a) Anti-wrinkle;

[0015] (b) firmness;

[0016] (c) Anti-aging.

[0017] The third aspect of the present invention provides a cosmetic having anti-wrinkle, firming and anti-aging effects, wherein the cosmetic comprises at least the above composition. More specifically, the cosmetic can be a skin care product.

[0018] A fourth aspect of the present invention provides a method for preparing the above-mentioned cosmetic, the method comprising mixing the composition with other raw material components.

[0019] Beneficial technical effects of one or more of the above technical solutions:

[0020] The above-mentioned composition containing 10-hydroxy-2-decenoic acid can significantly inhibit the generation of inflammatory factors, resist inflammatory aging, and there is a certain synergistic effect between the components, which can reduce the usage of 10-hydroxy-2-decenoic acid and reduce the cost of the formula. In addition, the above technical scheme unexpectedly found that 10-hydroxy-2-decenoic acid also has a certain effect of inhibiting lipid peroxidation, and the composition obtained by compounding it with its probiotic fermented brown algae and pterostilbene has a stronger effect of inhibiting lipid peroxidation, thereby effectively expanding the efficacy range of 10-hydroxy-2-decenoic acid. At the same time, the above technical scheme research found that compounding probiotic fermented brown algae, 10-hydroxy-2-decenoic acid and pterostilbene can effectively improve the illumination and thermal stability of pterostilbene, so it has good practical application value. DETAILED DESCRIPTION

[0021] It should be noted that the following detailed descriptions are all illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0022] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0023] The present invention is further described in conjunction with specific examples. The following examples are only for explaining the present invention and are not intended to limit the content thereof. If the specific experimental conditions are not specified in the examples, they are usually carried out according to conventional conditions or the conditions recommended by the reagent company; the reagents and consumables used in the following examples, unless otherwise specified, can all be obtained from commercial sources.

[0024] In a typical embodiment of the present invention, a composition containing 10-hydroxy-2-decenoic acid is provided, wherein the composition comprises the following components in parts by weight:

[0025] 0.1-50 parts of probiotic fermented brown algae, 0.001-0.5250 parts of pterostilbene, and 0.001-2 parts of 10-hydroxy-2-decenoic acid.

[0026] Among them, probiotic fermented brown algae is a composite component, containing more than four ingredients such as polysaccharides, oligosaccharides, amino acids, small peptides and glutathione, and has multiple skin care effects such as regulating skin microecology, repairing, anti-wrinkle, and anti-oxidation. It is obtained by multi-stage fermentation treatment using a variety of food-grade probiotics. In a specific embodiment of the present invention, the probiotic fermented brown algae is specifically a seaweed fermentation liquid obtained based on the fermentation method in CN 108653059 A. Further, the brown algae is kelp. At the same time, the probiotic fermented brown algae (i.e., seaweed fermentation liquid) does not contain live bacteria.

[0027] The pterostilbene, whose INCI name is Pterocarpus marsupium bark extract, is a methylated derivative of resveratrol, known as a new generation of resveratrol, and is widely distributed in plants such as red sandalwood, blueberry, strawberry, grape and aloe vera. According to domestic and foreign research reports, pterostilbene has multiple skin care effects such as whitening, anti-oxidation, anti-inflammatory, anti-glycation, protection against ultraviolet damage, and firming and anti-wrinkle, and is considered to be one of the most versatile whitening and anti-aging cosmetic ingredients. So far, no other natural plant ingredients have been found to surpass its efficacy, so pterostilbene is also known as the "king of plant whitening and antioxidant". However, the instability of pterostilbene itself greatly limits its application, because: pterostilbene belongs to polyphenols, has poor stability, is easily oxidized, and is easily degraded and isomerized when exposed to environmental conditions such as ultraviolet rays, oxygen, high temperature and pro-oxidants.

[0028] The 10-hydroxy-2-decenoic acid is royal jelly acid, and its source is not specifically limited.

[0029] Wherein, the 10-hydroxy-2-decenoic acid may be a pure product, or may be an aqueous solution, alcohol solution, liposome, ethosome or cyclodextrin inclusion body containing 10-hydroxy-2-decenoic acid. In a specific embodiment of the present invention, the 10-hydroxy-2-decenoic acid is an aqueous solution containing 10-hydroxy-2-decenoic acid, and its mass fraction (w / w) is 1-10%, and further 5%.

[0030] In another specific embodiment of the present invention, the composition comprises the following components in parts by weight:

[0031] 5 parts of probiotic fermented brown algae, 0.1 parts of pterostilbene, and 0.1 parts of 10-hydroxy-2-decenoic acid.

[0032] In another specific embodiment of the present invention, there is provided use of the above composition in preparing cosmetics.

[0033] The cosmetic has at least one or more of the following functions:

[0034] (a) Anti-wrinkle;

[0035] (b) firmness;

[0036] (c) Anti-aging.

[0037] Among them, in the application (c), anti-aging is specifically manifested as: inhibiting lipid peroxidation of the skin, thereby achieving the anti-aging effect.

[0038] In another specific embodiment of the present invention, a cosmetic having anti-wrinkle, firming and anti-aging effects is provided, wherein the cosmetic comprises at least the above composition. More specifically, the cosmetic may be a skin care product.

[0039] According to the cosmetic of the present invention, the composition is added in an amount of 0.5-10%, preferably 1-5%, such as 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, based on the total mass of the cosmetic.

[0040] The cosmetics may also contain any other raw material ingredients permitted to be added in the cosmetics field, including but not limited to emulsifiers, emollients, moisturizers, thickeners, preservatives, and the like.

[0041] At the same time, the present invention can also prepare different cosmetic dosage forms, such as water-based, emulsion, ointment (cream), etc. by reasonably adding the above-mentioned raw material ingredients. At the same time, based on the above-mentioned basic cosmetic categories, other cosmetic categories such as cleansing cream (ointment), facial cleanser, facial cream, lotion, facial mask, etc. are further derived and prepared, which are not specifically limited here.

[0042] In another specific embodiment of the present invention, a method for preparing the above cosmetic is provided, the preparation method comprising mixing the composition with other raw material components.

[0043] The present invention is further explained by the following examples, but it does not constitute a limitation of the present invention. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. The test methods in the following examples without specifying specific conditions are usually carried out under conventional conditions. The probiotic fermented brown algae in the examples of the present invention and the comparative examples are seaweed fermentation liquids obtained by the preparation method of Example 1 of CN 108653059 A.

[0044] The specific compositions of Examples 1 to 6 are as follows:

[0045]

[0046]

[0047] The specific compositions of Comparative Examples 1 to 7 are shown in the following table:

[0048]

[0049] Application Example 1 Emulsion Preparation

[0050] Phase A (parts by weight): 1 part of A165 (emulsifier), 3 parts of GTCC, 3 parts of white oil, 0.5 parts of cetostearyl alcohol, 5 parts of silicone oil, 0.1 parts of pterostilbene (pure powder);

[0051] Phase B (parts by weight): 4 parts of glycerol, 4 parts of butylene glycol, 0.4 parts of EMT-10 (thickener), 0.1 parts of xanthan gum, 0.4 parts of cinnamone, 0.4 parts of hexylene glycol, 0.2 parts of soy lecithin, and 100 parts of deionized water;

[0052] Phase C (parts by weight): 2 parts of 10-hydroxy-2-decenoic acid aqueous solution (5% by mass), 5 parts of probiotic fermented brown algae.

[0053] The above emulsion preparation steps are as follows:

[0054] 1) Disperse EMT-10, xanthan gum and soybean lecithin into glycerol and butylene glycol, then add fenvalerate, hexylene glycol and deionized water, heat and stir in a water bath at 80°C to fully dissolve, and obtain phase B;

[0055] 2) A165, GTCC, white oil, cetostearyl alcohol, silicone oil, and 0.1 part of pterostilbene (pure powder) were heated and melted in a water bath at 83° C. to obtain phase A;

[0056] 3) Heat phase A and phase B in a water bath at 83°C at the same time. When the two phases are at the same temperature, slowly add phase A to phase B while stirring phase B at 500 r / min.

[0057] 4) After phase B and phase A are mixed, the emulsion is cooled down under stirring conditions at 430 r / min;

[0058] 5) When the emulsion temperature drops to 40-45°C, add phase C into the system and stir at 60r / min to cool down;

[0059] 6) When the temperature dropped to 38°C, the mixture was cooled to obtain an emulsion containing the composition of Example 1.

[0060] Application Example 2 Preparation of facial cream

[0061] Phase A (parts by weight): 2.5 parts of A165 (emulsifier), 3 parts of GTCC, 3 parts of white oil, 1.5 parts of cetostearyl alcohol, 2 parts of shea butter, 5 parts of silicone oil, 0.1 parts of pterostilbene (pure powder);

[0062] Phase B (parts by weight): 4 parts of glycerol, 4 parts of butylene glycol, 0.4 parts of EMT-10 (thickener), 0.4 parts of sodium polyacrylate, 0.1 parts of xanthan gum, 0.4 parts of cinnamone, 0.4 parts of hexylene glycol, 0.2 parts of soybean lecithin, and 100 parts of deionized water;

[0063] Phase C (parts by weight): 2 parts of 10-hydroxy-2-decenoic acid aqueous solution (5% by mass), 5 parts of probiotic fermented brown algae.

[0064] The above emulsion preparation steps are as follows:

[0065] 1) Disperse EMT-10, sodium polyacrylate, xanthan gum and soybean lecithin into glycerol and butylene glycol, then add cinnamaldehyde, hexylene glycol and deionized water, heat and stir in a water bath at 80°C to fully dissolve, and obtain phase B;

[0066] 2) A165, GTCC, white oil, cetostearyl alcohol, silicone oil, shea butter, and 0.1 parts of pterostilbene (pure powder) were heated and melted in a water bath at 83° C. to obtain phase A;

[0067] 3) Heat phase A and phase B in a water bath at 83°C at the same time. When the two phases are at the same temperature, slowly add phase A to phase B while stirring phase B at 500 r / min.

[0068] 4) After phase B and phase A are mixed, the emulsion is cooled down under stirring conditions at 430 r / min;

[0069] 5) When the emulsion temperature drops to 40-45°C, add phase C into the system and stir at 60r / min to cool down;

[0070] 6) When the temperature drops to 38°C, cool the mixture to obtain a facial cream containing the composition of Example 1.

[0071] Experimental Example 1 Inflammatory Factors - TNF-α Inhibition Experiment

[0072] 1) Specific experimental methods for TNF-α detection are shown in Table 1 below:

[0073] Table 1

[0074]

[0075] Note: The sample solution is prepared by mixing the samples of the embodiment and the comparative embodiment with dimethyl sulfoxide (DMSO) to a total mass of 100 g.

[0076] 2) Preparation of working solution for positive control group (dexamethasone): 2 μL of 10% mother solution was dissolved in 2 mL of culture medium to prepare 0.01% dexamethasone.

[0077] 3) Cell seeding: 2.8×10 5 The cells were seeded into 6-well plates at a seeding density of 100 cells / well and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0078] 4) Drug administration: According to the experimental design in Table 1, when the cell plating rate in the 6-well plate reaches 40% to 60%, group drug administration is carried out, 2 mL of sample is added to each well, and 3 replicate wells are set for each group. After drug administration, the 6-well plate is placed in an incubator (37° C., 5% CO2) and cultured for 24 hours.

[0079] 5) UVB irradiation: After washing the cells with PBS, the UVB irradiated groups were irradiated with 300 mJ / cm 2 of UVB radiation.

[0080] 6) Post-incubation: After washing the cells with PBS, the 6-well plate was placed in an incubator (37° C., 5% CO 2 ) and incubated for 24 h.

[0081] 7) Collect cell supernatant: After 24 hours of incubation, collect the cell culture supernatant in an EP tube and place it in a -80°C refrigerator.

[0082] Keep frozen.

[0083] 8) ELISA test: Perform the test and analysis according to the operating instructions of the ELISA test kit.

[0084] 9) Calculation of TNF-α inhibition rate: The results are shown in Table 2:

[0085] Table 2 TNF-α inhibition rate in each group

[0086]

[0087]

[0088] Note: Significance is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0089] The test results show that the composition of the embodiment of the present invention can significantly inhibit the production of TNF-α, and the components have a certain synergistic effect, reducing the skin damage caused by inflammatory aging.

[0090] Experimental Example 2 Lipid Peroxidation-Malondialdehyde (MDA) Inhibition Detection

[0091] 1) Cell inoculation: After the cells are revived, when the plating rate reaches about 60%, the cells are inoculated into a 6-well plate and incubated overnight in a CO2 incubator (37°C, 5% CO2).

[0092] 2) Liquid preparation: Prepare the working solution of the test substance according to the test grouping, as shown in Table 3 below.

[0093] Table 3 Specific experimental design for lipid peroxidation detection

[0094]

[0095]

[0096] 3) Administration: According to the test grouping, when the cell plating rate in the 6-well plate reaches 30% to 50%, group administration is carried out, and 3 replicate wells are set up for each group. 2 mL of culture medium is added to each well of the blank control group and the negative control group, 2 mL of culture medium containing VE is added to each well of the positive control group, and 2 mL of culture medium containing the sample to be tested at the corresponding concentration is added to each well of the sample group. After the administration is completed, the 6-well plate is placed in a CO2 incubator (37°C, 5% CO2) and incubated for 24 hours.

[0097] 4) Irradiation: According to the test groups, except for the blank control group, the other groups were irradiated with UVB, and the irradiation dose was 300mJ / cm 2 After irradiation, the cells were placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24 hours.

[0098] 5) MDA detection: After the incubation, the cells were collected and lysed by adding 200 μL of NaOH to each tube, and then cooled in a 60°C water bath. After cooling, 62.5 μL of 35% perchloric acid was added to acidify the cells. The supernatant was collected by centrifugation and mixed thoroughly with 3 μL of 5 mM DNPH solution. The cells were placed at room temperature for 30 min in the dark and then detected by HPLC.

[0099] 6) Calculation of MDA down-regulation rate The results are shown in Table 4 below.

[0100] Table 4

[0101]

[0102]

[0103] Note: Significance is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0104] The test results show that the composition of the embodiment of the present invention can significantly reduce the content of MDA, and the components have a certain synergistic effect, which means that the composition can effectively inhibit lipid peroxidation of the skin, thereby achieving an anti-aging effect.

[0105] Experimental Example 3 Pterostilbene Stability Test

[0106] The solutions of Example 1 and Comparative Examples 1, 2, 4, 7, and 8 were respectively subjected to simultaneous illumination (illumination intensity 4500 Lx ± 500 Lx, 24 hours of uninterrupted illumination) and stability study at 45° C. for 3 weeks, and the residual rate of pterostilbene in the solution after 3 weeks was determined. The residual rate of pterostilbene was calculated according to the following formula:

[0107] Pterostilbene residual rate = (actual pterostilbene residual amount / initial pterostilbene content) × 100%

[0108] Among them, the method for determining the content of pterostilbene is as follows:

[0109] a. Drawing of pterostilbene standard curve

[0110] Accurately weigh 25.00 mg of pterostilbene standard and place it in a 10 mL volumetric flask. Add water and sonicate for 5 min to dissolve. Dose to volume and shake well to obtain a linear stock solution (mass concentration 2.00 mg mL -1 The linear stock solution was diluted with water to form a series of standard solutions with mass concentration gradients (0.05, 0.1, 0.2, 0.5, 1.0 and 2.0 mg·mL-1), filtered with a 0.22 μm filter membrane, and then tested by HPLC to record the peak area. The peak area (Y, mV·s) and pterostilbene concentration (ρ, mg·mL -1 ) standard curve, calculated the regression coefficient, and obtained the linear equation: Y=1655217.09ρ+7438.46, r=1, which has good linearity.

[0111] Among them, the HPLC detection conditions are: using Phenomenex C18 reverse phase bonded silica gel as the stationary phase, the size is 250mm×4.6mm, 5μm. The mobile phase is composed of acetonitrile and 1% glacial acetic acid, the ratio is 41:59. The flow rate is set to 1.5mL / min, the detection wavelength is set to 310nm, and the column temperature is maintained at 40℃.

[0112] b. Specificity investigation: The solution of Comparative Example 3 and Comparative Example 4 were tested in the above steps. The results showed that the peak positions of the two samples did not overlap, which indicated that the components in Comparative Example 3 had no interference with the determination of pterostilbene and the determination method had good specificity.

[0113] c. Determination of pterostilbene content in samples

[0114] 0.5 mL of the sample solution after 3 weeks of observation was accurately pipetted, filtered with a 0.22 μm filter membrane, and then subjected to HPLC detection. The peak area was recorded, and the actual pterostilbene residue was calculated according to the standard curve. The pterostilbene residue rate was also calculated. The results are shown in Table 5 below.

[0115] Table 5 Pterostilbene residual rate after samples were placed for 3 weeks (%)

[0116] Serial number name Pterostilbene residual rate under light irradiation / % Pterostilbene residual rate at 45℃ / % 1 Example 1 93.74 98.01 2 Comparative Example 1 63.68 70.29 3 Comparative Example 2 69.58 75.87 4 Comparative Example 4 60.28 68.87 5 Comparative Example 7 71.19 80.38 6 Comparative Example 8 94.32 98.32

[0117] As can be seen from the above table, under the conditions of light and high temperature of 45°C, the residual rate of pterostilbene decreases with the increase of time, which indicates that its stability is poor. However, after pterostilbene is compounded with the probiotic fermented brown algae and 10-hydroxy-2-decenoic acid provided by the present invention, the stability of pterostilbene under the conditions of light and high temperature of 45°C can be significantly improved. And compared with conventional antioxidants (vitamin E and pentaerythritol tetra (di-tert-butyl hydroxyhydrocinnamate), the stability improvement ability of the composition of the present invention on pterostilbene is better than vitamin E, and is equivalent to di-tert-butyl hydroxyhydrocinnamate.

[0118] Experimental Example 4 Evaluation of Anti-wrinkle Effect

[0119] Test sample: Skin care lotion of Application Example 1.

[0120] Subjects: Thirty healthy Chinese female subjects aged 30 to 40 years old were selected.

[0121] Test method: Use the sample twice a day, morning and evening, for 4 to 8 weeks.

[0122] The test results were collected at the following three time periods: before using the sample (D0), 4 weeks after using the sample, and 8 weeks after using the sample.

[0123] Test items: Cutometer (MPA580, Courage and Khazaka, Germany) was used to measure R2, R5 and R7 as parameters for evaluating skin elasticity. The results are shown in Table 6 below.

[0124] Table 6

[0125] Before use After 4 weeks of use After 8 weeks of use R2(mean) 0.512 0.698 0.585 R5(mean) 0.611 0.698 0.653 R7(mean) 0.323 0.468 0.403

[0126] Note: The larger the skin elasticity R2, R5, and R7 values ​​are, the better the skin elasticity is.

[0127] The results show that after using the emulsion containing the composition of the present invention, the skin elasticity is significantly improved, indicating that the composition of the present invention can be used to prepare cosmetics with anti-wrinkle, firming and anti-aging effects.

[0128] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition containing 10-hydroxy-2-decenoic acid, characterized in that, The composition comprises the following components in parts by weight: 0.1-50 parts of probiotic fermented brown algae, 0.001-0.5250 parts of pterostilbene, and 0.001-2 parts of 10-hydroxy-2-decenoic acid.

2. The composition according to claim 1, characterized in that The probiotic fermented brown algae is a seaweed fermentation liquid obtained based on the fermentation method in CN108653059A.

3. The composition according to claim 1, characterized in that The brown algae is kelp.

4. The composition according to claim 1, characterized in that The 10-hydroxy-2-decenoic acid is a pure product, or is any one or more of an aqueous solution, an alcohol solution, a liposome, an ethosome and a cyclodextrin inclusion body containing 10-hydroxy-2-decenoic acid.

5. The composition according to claim 4, characterized in that The 10-hydroxy-2-decenoic acid is an aqueous solution containing 10-hydroxy-2-decenoic acid, and the mass fraction (w / w) thereof is 1-10%, and further 5%.

6. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 5 parts of probiotic fermented brown algae, 0.1 parts of pterostilbene, and 0.1 parts of 10-hydroxy-2-decenoic acid.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of cosmetics, wherein the cosmetics have at least one or more of the following effects: (a) Anti-wrinkle; (b) firmness; (c) Anti-aging.

8. The use according to claim 7, characterized in that In the application (c), the anti-aging effect is specifically manifested as: inhibiting lipid peroxidation of the skin.

9. A cosmetic with anti-wrinkle, firming and anti-aging effects, characterized in that: The cosmetic comprises at least the composition according to any one of claims 1 to 6; further, the cosmetic is a skin care product.

10. The method for preparing the cosmetic according to claim 9, characterized in that: The preparation method comprises mixing the composition with other raw material components.

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