Anti-wrinkle repairing composition based on elastin peptide as well as preparation method and application of anti-wrinkle repairing composition

Abstract

The invention relates to the technical field of anti-wrinkle repair of skin, in particular to an anti-wrinkle repair composition based on elastin peptide as well as a preparation method and application of the anti-wrinkle repair composition. The composition is prepared from the following components in parts by weight: 1-15 parts of an elastin peptide solution, 1-5 parts of a morchella esculenta fermentation liquor, 1-5 parts of olive leaf polyphenol, 1-5 parts of a flavonoid compound, 1-5 parts of coffee residue polyphenol and 1-10 parts of cyclodextrin. According to the anti-wrinkle repairing composition based on the elastin peptide and the preparation method and application of the anti-wrinkle repairing composition, by resisting inflammation and oxidation and inhibiting melanin formation under light aging, the degradation amount of elastin in skin is reduced, aging states such as wrinkles and sunburn of the skin are delayed, and the skin is repaired.

Description

Technical Field

[0001] The invention relates to the technical field of skin anti-wrinkle repair, and in particular to an anti-wrinkle repair composition based on elastin peptide, and a preparation method and application thereof. Background Art

[0002] The skin is one of the largest and most complex organs in the human body. It consists of the epidermis, dermis, and subcutaneous fat tissue. The epidermis is the outermost layer and is the physical barrier of the skin. The dermis is responsible for the mechanical properties of the skin. It contains abundant fibroblasts that can synthesize and secrete extracellular matrix (ECM) components such as collagen and elastin. The subcutaneous fat tissue layer is the deepest layer of the skin, protecting the body from heat and cold and mechanical shock, as well as nutrient exchange and signal conduction.

[0003] Skin aging is divided into endogenous aging and exogenous aging. Endogenous aging is the inevitable result of genetic and physiological changes and is difficult to intervene. Exogenous aging refers to aging caused by environmental factors, such as ultraviolet radiation, sugar, tobacco, alcohol, chemicals, etc., which will add burden to the skin. As we age, the damage caused by this burden to the skin will become more significant and irreversible. Ultraviolet rays are the most important exogenous factor of skin aging. Long-term exposure to ultraviolet rays will cause certain damage to the structure and composition of the skin, leading to symptoms of photoaging such as wrinkles, dryness, and pigmentation.

[0004] Elastin peptides are degradation products of elastin, which participate in processes such as cell adhesion, chemotaxis, proliferation, and apoptosis, and have biological activities such as anti-skin aging and antioxidant activity. The content of elastin decreases sharply with age. In addition, the influence of external factors and diseases can also accelerate the process of elastin degradation, causing the skin to lose elasticity and show manifestations such as wrinkles and sagging skin. Therefore, there is an urgent need for a composition to delay skin aging and repair photo-aged skin. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-wrinkle repair composition based on elastin peptides, and a preparation method and application thereof, which can reduce the degradation of elastin in the skin, delay the appearance of wrinkles, sun spots and other aging conditions on the skin, and repair the skin through anti-inflammatory and anti-oxidation effects and inhibition of melanin formation under photoaging.

[0006] To achieve the above-mentioned purpose, the present invention provides an anti-wrinkle repair composition based on elastin peptide, a preparation method and application thereof, wherein the components include, by mass, 1-15 parts of elastin peptide solution, 1-5 parts of morel fermentation broth, 1-5 parts of olive leaf polyphenols, 1-5 parts of flavonoids, 1-5 parts of coffee grounds polyphenols and 1-10 parts of cyclodextrin.

[0007] Preferably, the elastin peptide is derived from the glomerulus arteriosus of deep-sea fish, and the molecular weight of the elastin peptide is 800-3000 Daltons.

[0008] Preferably, the flavonoids include one or more of osmanthus flavonoids, frangipani flavonoids and jasmine flavonoids.

[0009] The method for preparing the above-mentioned anti-wrinkle repair composition based on elastin peptide comprises the following steps: S1. preparing a cyclodextrin inclusion compound: dissolving cyclodextrin in water to obtain a first solution, dissolving olive leaf polyphenols, flavonoids and coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion compound; S2, preparing elastin peptide: taking the arterial bulb of deep-sea fish, washing it and beating it into slurry, adding elastin hydrolase to the slurry, enzymolysis to obtain enzymolysis solution, heating to terminate the enzymolysis, centrifuging and filtering, and dialyzing to obtain elastin peptide solution; S3, mixing the elastin peptide solution obtained in S2 with the Morchella fermentation liquid, and spray-drying to obtain a mixed powder; S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

[0010] Preferably, in S1, the magnetic stirring is stirring at 50-70°C for 20-30 h, and the temperature of the rotary evaporation is 30-50°C; Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

[0011] Preferably, in S1, the mass ratio of olive leaf polyphenols, flavonoids and coffee grounds polyphenols to cyclodextrin is 1:1-2.

[0012] Preferably, in S2, the amount of elastin hydrolase added is 0.5-2% of the mass of the slurry, and the elastin hydrolase includes one or more of papain, pancreatic elastase, elastase and metalloproteinase.

[0013] Preferably, in S2, a buffer solution is added during enzymolysis, the enzymolysis pH is 7.0-8.5, the enzymolysis temperature is 35-39° C., and the enzymolysis time is 1-24 h.

[0014] Preferably, in S3, spray drying is performed in a nitrogen atmosphere with a spray flow rate of 1-10 mL / min and a pressure of 1-10 bar.

[0015] The above-mentioned anti-wrinkle repairing composition based on elastin peptide is used in preparing facial cream, lotion and eye cream.

[0016] Therefore, the present invention adopts the above-mentioned components to form an anti-wrinkle repair composition based on elastin peptide, and its preparation method and application, and its beneficial effects are: 1. The present invention improves the solubility of olive leaf polyphenols, flavonoids and coffee grounds polyphenols in water when used by preparing cyclodextrin inclusion compounds, and increases the stability, while preventing olive leaf polyphenols, flavonoids and coffee grounds polyphenols from reacting with elastin peptides during the preparation of the anti-wrinkle repair composition; 2. Olive leaf polyphenols and coffee grounds polyphenols act as natural antioxidants, neutralizing free radicals to reduce skin damage and delay skin aging. They also have anti-inflammatory effects, reduce inflammation, and promote skin repair. 3. Osmanthus flavonoids, frangipani flavonoids and jasmine flavonoids have antioxidant and anti-inflammatory effects. At the same time, frangipani flavonoids have a moisturizing effect, osmanthus flavonoids stimulate the synthesis of collagen to enhance the elasticity and firmness of the skin, and the soothing effect of jasmine flavonoids can relieve skin sensitivity and redness. 4. The fermented liquid of Morchella has the effects of inhibiting the formation of melanin, soothing and repairing cells. Elastin peptide is a low-molecular active peptide extracted from elastin, which has the characteristics of anti-oxidation, moisture absorption and moisturizing, inhibiting tyrosinase activity, reducing the secretion of melanin and reducing melanin deposition; 5. The anti-wrinkle repair composition provided by the present invention reduces the degradation of elastin in the skin through anti-inflammatory and anti-oxidative effects and inhibits the formation of melanin under photoaging, delays the appearance of aging conditions such as wrinkles and sun spots on the skin, and repairs the skin.

[0017] The technical solution of the present invention is further described in detail below through embodiments. DETAILED DESCRIPTION

[0018] The present invention is further described below in conjunction with embodiments. Unless otherwise defined, the technical terms or scientific terms used in the present invention should be the common meanings understood by people with ordinary skills in the field to which the present invention belongs. The above-mentioned features or features mentioned in the specific examples mentioned in the present invention can be combined arbitrarily, and these specific embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0019] Example 1 A method for preparing an anti-wrinkle repair composition based on elastin peptide comprises the following steps: S1. Preparation of cyclodextrin inclusion complex: dissolving 8 parts of cyclodextrin in water to obtain a first solution, dissolving 2 parts of olive leaf polyphenols, 2 parts of flavonoids and 2 parts of coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion complex.

[0020] In S1, the magnetic stirring was at 60°C for 25 h, and the temperature of the rotary evaporation was 45°C.

[0021] Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

[0022] The flavonoid compounds are composed of osmanthus flavonoids and frangipani flavonoids in a mass ratio of 1:1.

[0023] S2. Preparation of elastin peptide: deep-sea fish bulbus arteriosus was washed and blended into slurry, elastin hydrolase was added to the slurry, enzymatic hydrolysis was performed to obtain enzymatic solution, the enzymatic hydrolysis was terminated by heating, the solution was filtered after centrifugation, and the elastin peptide solution was obtained after dialysis.

[0024] In S2, the amount of elastin hydrolase added was 1% of the mass of the slurry, and the elastin hydrolase consisted of papain and pancreatic elastase in a mass ratio of 0.5:1.

[0025] In S2, a buffer solution was added during enzymatic hydrolysis, the enzymatic hydrolysis pH was 7.5, the enzymatic hydrolysis temperature was 37°C, and the enzymatic hydrolysis time was 15 h.

[0026] S3. Mix 8 parts of the elastin peptide solution obtained in S2 with 5 parts of Morchella fermentation liquid, and spray-dry to obtain a mixed powder.

[0027] In S3, spray drying was performed in a nitrogen atmosphere with a spray flow rate of 8 mL / min and a pressure of 10 bar.

[0028] S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

[0029] Example 2 A method for preparing an anti-wrinkle repair composition based on elastin peptide comprises the following steps: S1. Preparation of cyclodextrin inclusion complex: dissolving 10 parts of cyclodextrin in water to obtain a first solution, dissolving 3 parts of olive leaf polyphenols, 3 parts of flavonoids and 3 parts of coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion complex.

[0030] In S1, the magnetic stirring was at 60°C for 25 h, and the temperature of the rotary evaporation was 45°C.

[0031] Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

[0032] The flavonoid compounds include osmanthus flavonoids and jasmine flavonoids in a mass ratio of 1:1.2.

[0033] S2. Preparation of elastin peptide: deep-sea fish bulbus arteriosus was washed and blended into slurry, elastin hydrolase was added to the slurry, enzymatic hydrolysis was performed to obtain enzymatic solution, the enzymatic hydrolysis was terminated by heating, the solution was filtered after centrifugation, and the elastin peptide solution was obtained after dialysis.

[0034] In S2, the amount of elastin hydrolase added was 1% of the mass of the slurry, and the elastin hydrolase consisted of papain and pancreatic elastase in a mass ratio of 1:1.

[0035] In S2, a buffer solution was added during enzymatic hydrolysis, the enzymatic hydrolysis pH was 7.5, the enzymatic hydrolysis temperature was 37°C, and the enzymatic hydrolysis time was 15 h.

[0036] S3. Mix 12 parts of the elastin peptide solution obtained in S2 with 3 parts of Morchella fermentation liquid, and spray-dry to obtain a mixed powder.

[0037] In S3, spray drying was performed in a nitrogen atmosphere with a spray flow rate of 8 mL / min and a pressure of 10 bar.

[0038] S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

[0039] Example 3 A method for preparing an anti-wrinkle repair composition based on elastin peptide comprises the following steps: S1. Preparation of cyclodextrin inclusion complex: dissolving 9 parts of cyclodextrin in water to obtain a first solution, dissolving 2 parts of olive leaf polyphenols, 3 parts of flavonoids and 3 parts of coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion complex.

[0040] In S1, the magnetic stirring was at 60°C for 25 h, and the temperature of the rotary evaporation was 45°C.

[0041] Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

[0042] The flavonoid compounds are composed of frangipani flavonoids and jasmine flavonoids in a mass ratio of 2:1.

[0043] S2. Preparation of elastin peptide: deep-sea fish bulbus arteriosus was washed and blended into slurry, elastin hydrolase was added to the slurry, enzymatic hydrolysis was performed to obtain enzymatic solution, the enzymatic hydrolysis was terminated by heating, the solution was filtered after centrifugation, and the elastin peptide solution was obtained after dialysis.

[0044] In S2, the amount of elastin hydrolase added was 1.2% of the mass of the slurry, and the elastin hydrolase consisted of papain and pancreatic elastase in a mass ratio of 0.8:1.

[0045] In S2, a buffer solution was added during enzymatic hydrolysis, the enzymatic hydrolysis pH was 7.5, the enzymatic hydrolysis temperature was 37°C, and the enzymatic hydrolysis time was 15 h.

[0046] S3. Mix 12 parts of the elastin peptide solution obtained in S2 with 3 parts of Morchella fermentation liquid, and spray-dry to obtain a mixed powder.

[0047] In S3, spray drying was performed in a nitrogen atmosphere with a spray flow rate of 8 mL / min and a pressure of 10 bar.

[0048] S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

[0049] Example 4 A method for preparing an anti-wrinkle repair composition based on elastin peptide comprises the following steps: S1. Preparation of cyclodextrin inclusion complex: dissolving 7 parts of cyclodextrin in water to obtain a first solution, dissolving 1.5 parts of olive leaf polyphenols, 3 parts of flavonoids and 2 parts of coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion complex.

[0050] In S1, the magnetic stirring was at 60°C for 25 h, and the temperature of the rotary evaporation was 45°C.

[0051] Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

[0052] The flavonoid compound is composed of osmanthus flavonoids and jasmine flavonoids in a mass ratio of 1:1.2.

[0053] S2. Preparation of elastin peptide: deep-sea fish bulbus arteriosus was washed and blended into slurry, elastin hydrolase was added to the slurry, enzymatic hydrolysis was performed to obtain enzymatic solution, the enzymatic hydrolysis was terminated by heating, the solution was filtered after centrifugation, and the elastin peptide solution was obtained after dialysis.

[0054] In S2, the amount of elastin hydrolase added was 0.9% of the mass of the slurry, and the elastin hydrolase consisted of papain and pancreatic elastase in a mass ratio of 1:1.

[0055] In S2, a buffer solution was added during enzymatic hydrolysis, the enzymatic hydrolysis pH was 7.5, the enzymatic hydrolysis temperature was 37°C, and the enzymatic hydrolysis time was 15 h.

[0056] S3. Mix 10 parts of the elastin peptide solution obtained in S2 with 2 parts of Morchella fermentation liquid, and spray-dry to obtain a mixed powder.

[0057] In S3, spray drying was performed in a nitrogen atmosphere with a spray flow rate of 8 mL / min and a pressure of 10 bar.

[0058] S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

[0059] Example 5 A method for preparing an anti-wrinkle repair composition based on elastin peptide comprises the following steps: S1. Preparation of cyclodextrin inclusion complex: dissolving 9 parts of cyclodextrin in water to obtain a first solution, dissolving 3 parts of olive leaf polyphenols, 3 parts of flavonoids and 3 parts of coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion complex.

[0060] In S1, the magnetic stirring was at 60°C for 25 h, and the temperature of the rotary evaporation was 45°C.

[0061] Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

[0062] The flavonoid compounds are composed of osmanthus flavonoids, frangipani flavonoids and jasmine flavonoids in a mass ratio of 1:1:1.

[0063] S2. Preparation of elastin peptide: deep-sea fish bulbus arteriosus was washed and blended into slurry, elastin hydrolase was added to the slurry, enzymatic hydrolysis was performed to obtain enzymatic solution, the enzymatic hydrolysis was terminated by heating, the solution was filtered after centrifugation, and the elastin peptide solution was obtained after dialysis.

[0064] In S2, the amount of elastin hydrolase added is 1% of the mass of the slurry, and the elastin hydrolase includes one or more of papain, pancreatic elastase, elastase and metalloproteinase.

[0065] In S2, a buffer solution was added during enzymatic hydrolysis, the enzymatic hydrolysis pH was 7.5, the enzymatic hydrolysis temperature was 37°C, and the enzymatic hydrolysis time was 15 h.

[0066] S3. Mix 15 parts of the elastin peptide solution obtained in S2 with 2 parts of Morchella fermentation liquid, and spray-dry to obtain a mixed powder.

[0067] In S3, spray drying was performed in a nitrogen atmosphere with a spray flow rate of 8 mL / min and a pressure of 10 bar.

[0068] S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

[0069] Application Example 1 The anti-wrinkle repairing composition prepared in Example 1 is applied to a facial cream. The preparation method of the facial cream comprises the following steps: T1. Prepare the aqueous phase: dissolve 10 parts of moisturizer, 3 parts of xanthan gum and 0.1 parts of preservative in water in sequence, heat in a water bath at 75° C., and stir to obtain the aqueous phase.

[0070] Among them, the moisturizers are glycerin, hyaluronic acid and hyaluronic acid, and the preservative is methylparaben.

[0071] T2. preparing an oil phase: the plant essential oils are rosemary essential oil, tea tree essential oil and lavender essential oil mixed in a mass ratio of 0.5:1:1, and then mixed with shea butter at 75° C. to obtain an oil phase.

[0072] T3. Dissolve 3 parts of surfactant in the water phase in S1, then slowly add into the oil phase in S2, continuously stir at 1500 r / min for 15 min, and then stir at a low speed of 500 r / min until cooled to room temperature to obtain a facial cream.

[0073] The surfactant is obtained by mixing lecithin and behenyl alcohol in a volume ratio of 1:1.

[0074] Application Example 2 The difference between this application example and application example 1 is that the anti-wrinkle repair composition prepared in Example 2 is used, and the rest is the same as in application example 1.

[0075] Application Example 3 The difference between this application example and application example 1 is that the anti-wrinkle repair composition prepared in Example 3 is used, and the rest is the same as in application example 1.

[0076] Application Example 4 The difference between this application example and application example 1 is that the anti-wrinkle repair composition prepared in Example 4 is used, and the rest is the same as in application example 1.

[0077] Application Example 5 The difference between this application example and application example 1 is that the anti-wrinkle repair composition prepared in Example 5 is used, and the rest is the same as in application example 1.

[0078] Comparative Example 1 The difference between this comparative example and Application Example 1 is that no anti-wrinkle repairing composition is added, and the rest is the same as in Application Example 1.

[0079] Test Example 1 a. Skin condition test a1. Trial population 60 women aged between 30 and 50 years old with healthy skin and no history of allergies.

[0080] a2. Test methods The test population was evenly divided into six groups, namely experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5 and control group, each group had 10 people. Each experimental group applied the same amount of the cream in Application Example 1, Application Example 2, Application Example 3, Application Example 4 and Application Example 5 after cleaning the skin every morning and evening, and the control group applied the cream in Comparative Example 1 after cleaning the skin, and conducted a 5-week elasticity test.

[0081] The elasticity test was conducted using the skin tester MPA580 to test the skin elasticity and pigmentation status of the test population. The test areas were the forehead, cheeks, around the eyes and around the lips. The average values ​​of the test results are shown in Tables 1 and 2.

[0082] Table 1. Data table of skin elasticity status of each group

[0083] Table 2. Data table of skin pigmentation status of each group

[0084] It can be seen from the data in Table 1 and Table 2 that the facial cream prepared using the anti-wrinkle repair composition is significantly better than the facial cream in Comparative Example 1 in terms of elasticity and pigmentation. It can be seen that the anti-wrinkle repair composition provided by the present invention reduces the degradation of elastin in the skin, delays the appearance of wrinkles, sun spots and other aging conditions on the skin, and repairs the skin through anti-inflammatory and antioxidant effects and inhibition of melanin formation under photoaging.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. An anti-wrinkle repair composition based on elastin peptide, characterized in that: The components include, by weight, 1-15 parts of elastin peptide solution, 1-5 parts of morel fermentation liquid, 1-5 parts of olive leaf polyphenols, 1-5 parts of flavonoids, 1-5 parts of coffee grounds polyphenols and 1-10 parts of cyclodextrin.

2. The anti-wrinkle repair composition based on elastin peptide according to claim 1, characterized in that: Elastin peptide is derived from the glomerulus arteriosus of deep-sea fish, and the molecular weight of elastin peptide is 800-3000 Daltons.

3. The anti-wrinkle repair composition based on elastin peptide according to claim 1, characterized in that: The flavonoids include one or more of osmanthus flavonoids, plumeria flavonoids and jasmine flavonoids.

4. A method for preparing an anti-wrinkle repair composition based on elastin peptide according to any one of claims 1 to 3, characterized in that: The following steps are included: S1. preparing a cyclodextrin inclusion compound: dissolving cyclodextrin in water to obtain a first solution, dissolving olive leaf polyphenols, flavonoids and coffee grounds polyphenols in ethanol to obtain a second solution, mixing the first solution and the second solution, stirring magnetically, rotary evaporating and filtering, concentrating the obtained filtrate and freeze-drying it to obtain a powdered cyclodextrin inclusion compound; S2, preparing elastin peptide: taking the arterial bulb of deep-sea fish, washing it and beating it into slurry, adding elastin hydrolase to the slurry, enzymolysis to obtain enzymolysis solution, heating to terminate the enzymolysis, centrifuging and filtering, and dialyzing to obtain elastin peptide solution; S3, mixing the elastin peptide solution obtained in S2 with the Morchella fermentation liquid, and spray-drying to obtain a mixed powder; S4, uniformly mixing the mixed powder with the cyclodextrin inclusion compound obtained in S1 to obtain an anti-wrinkle repairing composition.

5. The method for preparing an anti-wrinkle repair composition based on elastin peptide according to claim 4, characterized in that: In S1, the magnetic stirring was performed at 50-70 °C for 20-30 h, and the rotary evaporation temperature was 30-50 °C; Freeze drying is to pre-freeze at -40 to -50℃ for 2-4 h, heat to -30 to -10℃ and dry for 12-48 h, and finally heat to 0-30℃ and dry for 8-20 h.

6. The method for preparing an anti-wrinkle repair composition based on elastin peptide according to claim 4, characterized in that: In S1, the mass ratio of olive leaf polyphenols, flavonoids and coffee grounds polyphenols to cyclodextrin is 1:1-2.

7. The method for preparing an anti-wrinkle repair composition based on elastin peptide according to claim 4, characterized in that: In S2, the amount of elastin hydrolase added is 0.5-2% of the mass of the slurry, and the elastin hydrolase includes one or more of papain, pancreatic elastase, elastase and metalloproteinase.

8. The method for preparing an anti-wrinkle repair composition based on elastin peptide according to claim 4, characterized in that: In S2, a buffer solution is added during enzymolysis, the enzymolysis pH is 7.0-8.5, the enzymolysis temperature is 35-39°C, and the enzymolysis time is 1-24h.

9. The method for preparing an anti-wrinkle repair composition based on elastin peptide according to claim 4, characterized in that: In S3, spray drying is performed in a nitrogen atmosphere with a spray flow rate of 1-10 mL / min and a pressure of 1-10 bar.

10. The use of an anti-wrinkle repair composition based on elastin peptide according to any one of claims 1 to 3, characterized in that: Used in the preparation of facial creams, lotions and eye creams.

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