Cell culture device

Abstract

The cell culture device comprises a shell, the top in the shell is divided into a plurality of fan-shaped cavities through a plurality of fixedly-connected partition plates, rectangular openings are formed in the bottoms of the partition plates, movable plates are slidably mounted in the rectangular openings, and the bottom ends of the movable plates slidably abut against placement parts; the placing part is rotationally installed in the shell, the center bottom end of the placing part is fixedly connected with the transmission assembly, the transmission assembly is rotationally installed in the cylindrical cavity, the cylindrical cavity is formed in the bottom in the shell in a hollow mode, the transmission assembly is fixedly connected with the servo motor, and the servo motor is fixedly installed in the cylindrical cavity; an output shaft of the servo motor is in transmission connection with a transmission shaft through a transmission belt, the transmission shaft is rotationally installed on one side in the shell, the side, away from the servo motor, of the transmission shaft is in transmission connection with a cleaning rotating shaft through a transmission belt, the cleaning rotating shaft is rotationally installed in the fan-shaped cavity, and one side of the cleaning rotating shaft is provided with a first electric telescopic rod fixedly connected with the inner wall of the fan-shaped cavity. The end of the first electric telescopic rod is fixedly connected with a mounting plate.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions.

[0003] Usually, cell culture is carried out in culture dishes, which are usually set up independently to avoid mixing of cells in the culture dishes. The culture medium used for cell culture in the culture dishes also needs to be replaced regularly. The traditional manual replacement not only requires taking out each culture dish one by one for individual replacement, but also each time the culture dish is opened to change the medium, the environment in the culture dish may be damaged, which has a negative impact on cell culture. In addition, manual operation is also cumbersome, which increases the labor burden and is also inefficient. For this reason, we propose a cell culture device to solve the above problems. Summary of the invention

[0004] The object of the present invention is to provide a cell culture device to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a cell culture device, comprising a shell, the top of the shell is divided into a plurality of fan-shaped cavities by a plurality of fixed partitions, the bottom of the partition is provided with a rectangular opening and a movable plate is slidably installed in the rectangular opening, the bottom end of the movable plate slides against a placement portion, the placement portion is rotatably installed in the shell and the central bottom end of the placement portion is fixedly connected to a transmission component, the transmission component is rotatably installed in a cylindrical cavity, the cylindrical cavity is hollow and arranged at the bottom of the shell, the transmission component is fixedly connected to a servo motor, the servo motor is fixedly installed in the cylindrical cavity, the output shaft of the servo motor is connected to a transmission shaft through a transmission belt, the transmission shaft is rotatably installed on one side of the shell, the side of the transmission shaft away from the servo motor is connected to a cleaning shaft through a transmission belt, the cleaning shaft is rotatably installed in the fan-shaped cavity, one side of the cleaning shaft is provided with an electric telescopic rod 1 fixedly connected to the inner wall of the fan-shaped cavity, the end of the electric telescopic rod 1 is fixedly connected to a mounting plate, and one side of the mounting plate is fixedly connected to a liquid extraction tube.

[0006] Preferably, at least five fan-shaped cavities are evenly distributed around the circumference, the top inner walls of two fan-shaped cavities are respectively fixedly connected to the second electric telescopic rod, and the driving ends of the second electric telescopic rod are respectively fixedly connected to suction cups.

[0007] Preferably, an electric telescopic rod 1 is respectively provided in two adjacent fan-shaped cavities, one end of the electric telescopic rod 1 is fixedly connected to the mounting plate and the liquid extraction tube, and the other end of the electric telescopic rod 1 is fixedly connected to the mounting plate and the liquid infusion tube. The top of the shell is fixedly connected to a liquid supply box, and the liquid supply box is connected to the liquid infusion tube through a pipeline.

[0008] Preferably, a transmission cavity is provided in the top hollow of the shell, and the transmission cavity is arranged inside the fan-shaped cavity.

[0009] Preferably, the placement portion includes a placement plate, a connecting strip, an outer convex strip, an adjustable clamping assembly, an inner convex strip, a transmission connecting rod, a transmission ring, a connecting block, a transmission shaft 1, and an arc strip, the transmission shaft is coaxially rotatably installed in the transmission cavity, one end of the transmission connecting shaft 1 is fixedly connected to the transmission assembly, the outer wall of the transmission connecting shaft 1 is fixedly connected to one end of the connecting block, the other end of the connecting block is fixedly connected to the transmission ring, the transmission ring is rotatably connected to the shell, the outer side of the transmission ring is fixedly connected to one end of the transmission connecting rod, and the other end of the transmission connecting rod is fixedly connected to the placement plate, the placement plate and the transmission connecting rod are both arranged in the fan-shaped cavity, one side edge of the placement plate is fixedly connected to the outer convex strip, the other side edge of the placement plate is fixedly connected to the inner convex strip, the end of the inner convex strip opposite to the outer convex strip is fixedly connected to the arc strip, the side of the arc strip away from the inner convex strip is movably provided with an adjustable clamping assembly, and the two sides between two adjacent placement plates are respectively fixedly connected to the connecting strips.

[0010] Preferably, both ends of the outer convex strip and the inner convex strip are respectively provided with slope surfaces, the outer convex strip and the inner convex strip have the same bending direction, and the outer convex strip and the inner convex strip are concentrically arranged.

[0011] Preferably, the adjustable clamping assembly includes a movable arc strip, a threaded rod, an internally threaded barrel, an adjusting hand wheel, and a groove; the movable arc strip is slidably arranged on the top surface of the placement plate; the middle part of one end of the movable arc strip is fixedly connected to one end of the threaded rod; the other end of the threaded rod is sleeved with the internally threaded barrel through a threaded structure; the internally threaded barrel is rotatably connected to the external convex strip and the end of the internally threaded barrel is fixedly connected to the adjusting hand wheel; the adjusting hand wheel is rotatably arranged in the groove; and the groove is concavely arranged on the external convex strip.

[0012] Preferably, the transmission assembly includes an active shaft, an active disk, an active connecting rod, a toggle block, a transmission disk, and a second transmission shaft. The active shaft is rotatably installed in a cylindrical cavity, one end of the active shaft is fixedly connected to a servo motor, the active shaft is fixedly connected to one side of the active connecting rod, the other side of the active connecting rod is rotatably connected to the toggle block, one side of the active shaft is fixedly sleeved on the active disk, one side of the active disk slides against the transmission disk, the transmission disk is fixedly sleeved on the second transmission shaft, and one end of the second transmission shaft is fixedly connected to the end of the first transmission shaft.

[0013] Preferably, the active connecting rod is arranged between the active disk and the servo motor, and a plurality of limit openings are opened on the outer edge of the transmission disk, and the limit openings slide against the active disk, and a toggle opening is opened between two adjacent limit openings, and the toggle opening is slidably engaged with the toggle block, and a fan-shaped opening is opened on one side of the active disk, and the active connecting rod is arranged in the middle of the fan-shaped opening.

[0014] Preferably, a cleaning rod is fixedly connected to the end of the cleaning shaft, and a cleaning sponge is bonded to the cleaning rod.

[0015] Compared with the prior art, the present invention has the following beneficial effects: by arranging a plurality of independent fan-shaped cavities inside the shell, when the culture dishes are placed in the shell, the culture dishes are independent of each other, thereby avoiding cross contamination of the culture dishes on the placement portion; the servo motor drives the transmission component to work, and the transmission component drives the placement portion to rotate intermittently, thereby switching the culture dishes on the placement portion, thereby facilitating the operations of opening the lid of the culture dish, extracting liquid, infusing liquid, and closing the lid; at the same time, each process is separated from each other by a partition plate in cooperation with a movable plate, thereby avoiding cell contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the present invention;

[0017] Figure 2 It is a schematic diagram of the cross-sectional structure of the present invention;

[0018] Figure 3 It is a schematic diagram of the cross-sectional structure of the present invention from another viewing angle;

[0019] Figure 4 It is a schematic diagram of the cross-sectional structure of the present invention;

[0020] Figure 5 For the present invention Figure 3 A schematic diagram of the structure enlargement in the middle;

[0021] Figure 6 It is a schematic diagram of the structure of the placement part in the present invention;

[0022] Figure 7 It is a schematic diagram of the structure of the transmission component in the present invention.

[0023] In the figure: housing 1, fan-shaped cavity 101, cylindrical cavity 102, transmission cavity 103, partition 2, movable plate 3, placement part 4, placement plate 41, connecting strip 42, outer convex strip 43, adjustable clamping assembly 44, movable arc strip 441, threaded rod 442, inner threaded cylinder 443, adjusting hand wheel 444, groove 445, inner convex strip 45, transmission connecting rod 46, transmission ring 47, connecting block 48, transmission shaft 1 49, arc strip 40 , culture dish 5, liquid supply box 6, servo motor 7, transmission shaft 8, cleaning shaft 9, cleaning rod 91, electric telescopic rod one 10, mounting plate 11, liquid extraction tube 12, transmission assembly 13, active shaft 131, active disk 132, fan-shaped mouth 1321, active connecting rod 133, toggle block 134, transmission disk 135, limit mouth 1351, toggle mouth 1352, transmission shaft two 136, electric telescopic rod two 14, suction cup 15. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] Reference Figure 1 , 2 , which is the first embodiment of the present invention, provides a cell culture device, including a shell 1, the top of the shell 1 is divided into a plurality of fan-shaped cavities 101 by a plurality of fixed partitions 2, the bottom of the partition 2 is provided with a rectangular opening and a movable plate 3 is slidably installed in the rectangular opening, the bottom end of the movable plate 3 slides against the placement portion 4, the placement portion 4 is rotatably installed in the shell 1 and the central bottom end of the placement portion 4 is fixedly connected to a transmission component 13, the transmission component 13 is rotatably installed in a columnar cavity 102, the columnar cavity 102 is hollow and arranged at the bottom of the shell 1, and the transmission component 13 The servo motor 7 is fixedly connected and fixedly installed in the cylindrical cavity 102. The output shaft of the servo motor 7 is connected to the transmission shaft 8 through a transmission belt. The transmission shaft 8 is rotatably installed on one side of the shell 1. The side of the transmission shaft 8 away from the servo motor 7 is connected to the cleaning shaft 9 through a transmission belt. The cleaning shaft 9 is rotatably installed in the fan-shaped cavity 101. One side of the cleaning shaft 9 is provided with an electric telescopic rod 10 fixedly connected to the inner wall of the fan-shaped cavity 101. The end of the electric telescopic rod 10 is fixedly connected to a mounting plate 11, and one side of the mounting plate 11 is fixedly connected to a liquid extraction tube 12.

[0027] The shell 1 is provided with a plurality of fan-shaped doors, which are convenient for placing culture dishes 5 and the like inside the shell 1. The culture dishes 5 are placed on the placement portion 4, which clamps and fixes the culture dishes 5. When the culture dishes 5 need to be replaced, the servo motor 7 is powered on, and the servo motor 7 drives the transmission assembly 13 to work. The transmission assembly 13 cooperates with the placement portion 4 to drive the culture dishes 5 to switch positions intermittently. At the same time, the movable plate 3 is lifted when switching positions to avoid blocking the placement portion 4, so that the placement plate 41 can be smoothly transferred to the adjacent fan-shaped cavity 101. When the placement plate 41 is completely transferred to the adjacent fan-shaped cavity 101, the movable plate 3 falls along the slope surface of the inner convex strip 45 and the outer convex strip 43, thereby sealing the fan-shaped cavity 101, so that the fan-shaped cavities 101 are independent of each other, and then the electric telescopic rod 14 works to drive the suction cup 15 to descend. After the suction cup 15 descends, it adsorbs the dish cover of the culture dish 5, and then the electric telescopic rod 14 is lifted to separate the adsorbed dish cover from the culture dish 5, and then After that, the servo motor 7 works again, and cooperates with the transmission component 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the uncovered culture dish 5 is transferred to the next fan-shaped cavity 101, the electric telescopic rod 10 descends, driving the liquid extraction tube 12 to descend, and the nozzle of the liquid extraction tube 12 extends into the culture fluid, and then the liquid extraction pump works to extract the culture fluid that needs to be replaced. After the extraction is completed, the servo motor 7 works again, and cooperates with the transmission component 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the culture dish 5 without culture fluid is transferred to the next fan-shaped cavity 101, the infusion tube extends above the culture dish 5, and then the culture fluid is injected into the culture dish 5. After the culture fluid is injected, the placement plate 41 rotates again to the next fan-shaped cavity 101. The uncovered culture dish 5 injected with culture fluid is covered with a dish cover again under the drive of the electric telescopic rod 3 to keep the culture dish 5 intact. At the same time, each process is independent of each other to prevent cell contamination.

[0028] Example 2

[0029] Reference Figure 1-7 , which is the second embodiment of the present invention. This embodiment is based on the previous embodiment. Specifically, at least five fan-shaped cavities 101 are evenly distributed around the circumference. The top inner walls of the two fan-shaped cavities 101 are respectively fixedly connected to the electric telescopic rods 14. The driving ends of the electric telescopic rods 14 are respectively fixedly connected to the suction cups 15. The arrangement of multiple fan-shaped cavities 101 facilitates the separate implementation of different processes. The suction cups 15 adsorb the dish cover of the culture dish 5.

[0030] Specifically, two adjacent fan-shaped cavities 101 are respectively provided with electric telescopic rods 10, the end of one electric telescopic rod 10 is fixedly connected to the mounting plate 11 and the liquid extraction tube 12, and the end of the other electric telescopic rod 10 is fixedly connected to the mounting plate 11 and the liquid infusion tube. The top of the shell 1 is fixedly connected to the liquid supply box 6, and the liquid supply box 6 is connected to the liquid infusion tube through a pipeline.

[0031] The electric telescopic rod 14 works, driving the suction cup 15 to descend. After the suction cup 15 descends, it adsorbs the dish cover of the culture dish 5. Then the electric telescopic rod 14 is lifted to separate the adsorbed dish cover from the culture dish 5. Then the servo motor 7 works again, cooperates with the transmission component 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the uncovered culture dish 5 is transferred to the next fan-shaped cavity 101, the electric telescopic rod 10 descends, driving the liquid extraction tube 12 to descend. The tube mouth of the liquid extraction tube 12 extends into the culture liquid, and then the liquid extraction pump works to extract the culture liquid that needs to be replaced. After the extraction is completed, After completion, the servo motor 7 works again, and cooperates with the transmission component 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the culture dish 5 without culture fluid is transferred to the next fan-shaped cavity 101, the infusion tube is extended to the top of the culture dish 5, and then the culture fluid is injected into the culture dish 5. After the injection of the culture fluid is completed, the placement plate 41 is rotated to the next fan-shaped cavity 101 again. The uncovered culture dish 5 injected with culture fluid is driven by the electric telescopic rod 3, and the culture dish 5 is covered with a dish cover again to keep the integrity of the culture dish 5. At the same time, each process is independent of each other to prevent cell contamination.

[0032] Specifically, a transmission cavity 103 is provided in the hollow top of the shell 1 , and the transmission cavity 103 is provided inside the fan-shaped cavity 101 . The transmission cavity 103 is coaxially arranged with the shell 1 , and a transmission shaft 49 is rotatably installed in the transmission cavity 103 .

[0033] Specifically, the placement portion 4 includes a placement plate 41, a connecting strip 42, an outer convex strip 43, an adjustable clamping assembly 44, an inner convex strip 45, a transmission connecting rod 46, a transmission ring 47, a connecting block 48, a transmission shaft 49, and an arc strip 40. The transmission shaft 49 is coaxially rotatably installed in the transmission cavity 103, one end of the transmission shaft 49 is fixedly connected to the transmission assembly 13, the outer wall of the transmission shaft 49 is fixedly connected to one end of the connecting block 48, and the other end of the connecting block 48 is fixedly connected to the transmission ring 47. The transmission ring 47 is rotatably connected to the housing 1, and the transmission ring 47 is One end of the transmission link 46 is fixedly connected to the outside, and the other end of the transmission link 46 is fixedly connected to the placement plate 41. The placement plate 41 and the transmission link 46 are both arranged in the fan-shaped cavity 101. One side edge of the placement plate 41 is fixedly connected to the outer convex strip 43, and the other side edge of the placement plate 41 is fixedly connected to the inner convex strip 45. The end of the inner convex strip 45 opposite to the outer convex strip 43 is fixedly connected to the arc strip 40. An adjustable clamping component 44 is movably provided on the side of the arc strip 40 away from the inner convex strip 45. The two sides between the two adjacent placement plates 41 are respectively fixedly connected to the connecting strips 42.

[0034] Furthermore, both ends of the outer convex strip 43 and the inner convex strip 45 are respectively provided with slope surfaces, the outer convex strip 43 and the inner convex strip 45 have the same bending direction, and the outer convex strip 43 and the inner convex strip 45 are concentrically arranged.

[0035] Furthermore, the adjustable clamping assembly 44 includes a movable arc bar 441, a threaded rod 442, an internal threaded barrel 443, an adjusting hand wheel 444, and a groove 445. The movable arc bar 441 is slidably arranged on the top surface of the placement plate 41. The middle part of one end of the movable arc bar 441 is fixedly connected to one end of the threaded rod 442. The other end of the threaded rod 442 is sleeved with the internal threaded barrel 443 through a threaded structure. The internal threaded barrel 443 is rotatably connected to the outer convex strip 43 and the end of the internal threaded barrel 443 is fixedly connected with an adjusting hand wheel 444. The adjusting hand wheel 444 is rotatably arranged in the groove 445, and the groove 445 is concavely arranged on the outer convex strip 43.

[0036] The culture dish 5 is placed on the placement plate 41 of the placement part 4, and the adjustable clamping assembly 44 is operated to rotate the adjusting hand wheel 444 to drive the fixed internal threaded cylinder 443 to rotate. The internal threaded cylinder 443 drives the threaded rod 442 to move through the threaded structure. The threaded rod 442 drives the movable arc bar 441 fixed at the end to move toward the arc bar 40. The movable arc bar 441 cooperates with the arc bar 40 to clamp and fix the culture dish 5. When the culture dish 5 needs to be replaced, the servo motor 7 is energized. The servo motor 7 drives the transmission assembly 13 to work, the active shaft 131 of the transmission assembly 13 rotates, the active shaft 131 drives the fixed active connecting rod 133 to rotate in a circle, the active connecting rod 133 drives the toggle block 134 to rotate in a circle, the toggle block 134 rotates to the position of the transmission disk 135 and enters the toggle opening 1352, the toggle block 134 then drives the transmission disk 135 to rotate, the transmission disk 135 drives the fixed transmission shaft 2 136 to rotate, the transmission shaft 2 136 drives The placement portion 4 rotates, the transmission shaft 49 of the placement portion 4 rotates, the transmission shaft 49 drives the fixed connection block 48 to rotate, the connection block 48 drives the fixed transmission ring 47 to rotate, the transmission ring 47 drives the fixed transmission connecting rod 46 to rotate, the transmission connecting rod 46 drives the fixed placement plate 41 to rotate, the placement plate 41 then drives the fixed connection strip 42, the inner convex strip 45, and the outer convex strip 43 to rotate synchronously, when the inner convex strip 45 and the outer convex strip 43 rotate toward the movable plate 3, the inner convex strip 45 and the outer convex strip 43 rotate toward the movable plate 3. The reverse force of the outer convex strip 43 drives the movable plate 3 in sliding resistance to lift up, and the bottom end of the movable plate 3 is fixedly connected to the sealing rubber block to seal the two adjacent fan-shaped cavities 101. After the movable plate 3 is lifted up, the placement plate 41 can be smoothly transferred to the adjacent fan-shaped cavity 101. When the placement plate 41 is completely transferred to the adjacent fan-shaped cavity 101, the movable plate 3 falls along the slope surface of the inner convex strip 45 and the outer convex strip 43, thereby sealing the fan-shaped cavity 101 and making the fan-shaped cavities 101 independent of each other.

[0037] Specifically, the transmission assembly 13 includes an active shaft 131, an active disk 132, an active connecting rod 133, a toggle block 134, a transmission disk 135, and a second transmission shaft 136. The active shaft 131 is rotatably installed in the cylindrical cavity 102, one end of the active shaft 131 is fixedly connected to the servo motor 7, the active shaft 131 is fixedly connected to one side of the active connecting rod 133, and the other side of the active connecting rod 133 is rotatably connected to the toggle block 134. One side of the active shaft 131 is fixedly sleeved on the active disk 132, one side of the active disk 132 slides against the transmission disk 135, the transmission disk 135 is fixedly sleeved on the second transmission shaft 136, and one end of the second transmission shaft 136 is fixedly connected to the end of the first transmission shaft 49.

[0038] Furthermore, the active connecting rod 133 is arranged between the active disk 132 and the servo motor 7, and a plurality of limit openings 1351 are opened on the outer edge of the transmission disk 135, and the limit openings 1351 slide against the active disk 132. A toggle opening 1352 is opened between two adjacent limit openings 1351, and the toggle opening 1352 is slidably sleeved with the toggle block 134. A fan-shaped opening 1321 is opened on one side of the active disk 132, and the active connecting rod 133 is arranged in the middle of the fan-shaped opening 1321.

[0039] The electric telescopic rod 14 works, driving the suction cup 15 to descend. After the suction cup 15 descends, it adsorbs the dish cover of the culture dish 5. Then the electric telescopic rod 14 is lifted to separate the adsorbed dish cover from the culture dish 5. Then the servo motor 7 works again, cooperates with the transmission component 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the uncovered culture dish 5 is transferred to the next fan-shaped cavity 101, the electric telescopic rod 10 descends, driving the liquid extraction tube 12 to descend. The tube mouth of the liquid extraction tube 12 extends into the culture liquid, and then the liquid extraction pump works to extract the culture liquid that needs to be replaced. After the extraction is completed, After completion, the servo motor 7 works again, and cooperates with the transmission component 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the culture dish 5 without culture fluid is transferred to the next fan-shaped cavity 101, the infusion tube is extended to the top of the culture dish 5, and then the culture fluid is injected into the culture dish 5. After the injection of the culture fluid is completed, the placement plate 41 is rotated to the next fan-shaped cavity 101 again. The uncovered culture dish 5 injected with culture fluid is driven by the electric telescopic rod 3, and the culture dish 5 is covered with a dish cover again to keep the integrity of the culture dish 5. At the same time, each process is independent of each other to prevent cell contamination.

[0040] Specifically, a cleaning rod 91 is fixedly connected to the end of the cleaning shaft 9, and a cleaning sponge is bonded to the cleaning rod 91. When the transmission shaft 8 rotates, the transmission drives the cleaning shaft 9 to rotate, and the cleaning shaft 9 drives the cleaning rod 91 to rotate in a circle. When the cleaning rod 91 rotates, the bonded cleaning sponge slides against the output port of the suction tube 12 and the infusion tube, thereby cleaning and disinfecting the suction tube 12 and the infusion tube. At the same time, the cleaning sponge is replaced regularly to avoid cross contamination.

[0041] Example 3

[0042] Reference Figure 1-7, which is the third embodiment of the present invention. This embodiment is based on the above two embodiments. When in use, a plurality of fan-shaped doors are respectively installed on the shell 1, which are convenient for placing a culture dish 5 inside the shell 1. The culture dish 5 is placed on the placement plate 41 of the placement portion 4. The adjustable clamping assembly 44 is operated, and the adjusting hand wheel 444 is rotated to drive the fixed internal threaded cylinder 443 to rotate. The internal threaded cylinder 443 drives the threaded rod 442 to move through the threaded structure. The threaded rod 442 drives the movable arc bar 441 fixed at the end to move toward the arc bar 40. The movable arc bar 441 cooperates with the arc bar 40 to clamp and fix the culture dish 5. When the culture dish 5 needs to be replaced, the servo motor 7 is powered on, and the servo motor 7 drives the transmission assembly 13 to work. The transmission assembly 13 The active rotating shaft 131 rotates, and the active rotating shaft 131 drives the fixed active connecting rod 133 to rotate in a circle. The active connecting rod 133 drives the toggle block 134 to rotate in a circle. After the toggle block 134 rotates to the position of the transmission disk 135, it enters the toggle opening 1352. The toggle block 134 then drives the transmission disk 135 to rotate. The transmission disk 135 drives the fixed transmission rotating shaft 2 136 to rotate. The transmission rotating shaft 2 136 drives the placement part 4 to rotate. The transmission rotating shaft 1 49 of the placement part 4 rotates. The transmission rotating shaft 1 49 drives the fixed connecting block 48 to rotate. The connecting block 48 drives the fixed transmission ring 47 to rotate. The transmission ring 47 drives the fixed transmission connecting rod 46 to rotate. The transmission connecting rod 46 drives the fixed placement plate 41 to rotate. The placement plate 41 then drives the fixed connecting strip 42, the inner convex strip 45 and the outer convex strip 43 rotate synchronously. When the inner convex strip 45 and the outer convex strip 43 rotate toward the movable plate 3, the reverse force of the inner convex strip 45 and the outer convex strip 43 drives the movable plate 3 that is in sliding contact to lift up. The bottom end of the movable plate 3 is fixedly connected to the sealing rubber block to seal the two adjacent fan-shaped cavities 101. After the movable plate 3 is lifted, the placement plate 41 can be smoothly transferred to the adjacent fan-shaped cavity 101. When the placement plate 41 is completely transferred to the adjacent fan-shaped cavity 101, the movable plate 3 falls along the slope surface of the inner convex strip 45 and the outer convex strip 43, thereby sealing the fan-shaped cavity 101 and making the fan-shaped cavities 101 independent of each other. Then the electric telescopic rod 14 works to drive the suction cup 15 to descend. After the suction cup 15 descends, the dish cover of the culture dish 5 is closed. After the culture dish 5 without the culture fluid is transferred to the next fan-shaped cavity 101, the electric telescopic rod 10 descends, driving the liquid extraction tube 12 to descend, and the tube mouth of the liquid extraction tube 12 extends into the culture fluid, and then the liquid extraction pump works to extract the culture fluid that needs to be replaced. After the extraction is completed, the servo motor 7 works again, and cooperates with the transmission assembly 13 to drive the placement plate 41 to rotate again to the next fan-shaped cavity 101. After the culture dish 5 without the culture fluid is transferred to the next fan-shaped cavity 101, the infusion tube extends to the top of the culture dish 5, and then the culture fluid is injected into the culture dish 5.After the culture fluid is injected, the placement plate 41 rotates again to the next fan-shaped cavity 101, and the uncovered culture dish 5 injected with the culture fluid is covered with a dish cover again by the electric telescopic rod 3 to keep the culture dish 5 intact. At the same time, each process is independent of each other to prevent cell contamination.

[0043] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture device, comprising a housing (1), characterized in that: The top of the shell (1) is divided into a plurality of fan-shaped cavities (101) by a plurality of fixed partitions (2); a rectangular opening is provided at the bottom of the partition (2) and a movable plate (3) is slidably installed in the rectangular opening; the bottom end of the movable plate (3) slidably contacts the placement portion (4); the placement portion (4) is rotatably installed in the shell (1) and the central bottom end of the placement portion (4) is fixedly connected to a transmission component (13); the transmission component (13) is rotatably installed in a columnar cavity (102); the columnar cavity (102) is hollow and arranged at the bottom of the shell (1); the transmission component (13) is fixedly connected to a servo motor (7); the servo motor (7) The cleaning shaft (9) is rotatably mounted in the fan-shaped cavity (101). One side of the cleaning shaft (9) is provided with an electric telescopic rod (10) fixedly connected to the inner wall of the fan-shaped cavity (101). The end of the electric telescopic rod (10) is fixedly connected to a mounting plate (11). One side of the mounting plate (11) is fixedly connected to a liquid extraction tube (12).

2. A cell culture device according to claim 1, characterized in that: At least five fan-shaped cavities (101) are evenly distributed around the circumference, and the top inner walls of two fan-shaped cavities (101) are respectively fixedly connected to the second electric telescopic rod (14), and the driving ends of the second electric telescopic rod (14) are respectively fixedly connected to a suction cup (15).

3. A cell culture device according to claim 1, characterized in that: An electric telescopic rod (10) is respectively provided in two adjacent fan-shaped cavities (101); the end of one electric telescopic rod (10) is fixedly connected to a mounting plate (11) and a liquid extraction tube (12); the end of the other electric telescopic rod (10) is fixedly connected to a mounting plate (11) and a liquid infusion tube; the top end of the housing (1) is fixedly connected to a liquid supply box (6); the liquid supply box (6) is connected to the liquid infusion tube via a pipeline.

4. A cell culture device according to claim 1, characterized in that: A transmission cavity (103) is provided in the hollow top of the housing (1), and the transmission cavity (103) is arranged inside the fan-shaped cavity (101).

5. A cell culture device according to claim 4, characterized in that: The placement portion (4) comprises a placement plate (41), a connecting strip (42), an outer convex strip (43), an adjustable clamping assembly (44), an inner convex strip (45), a transmission connecting rod (46), a transmission ring (47), a connecting block (48), a transmission shaft 1 (49), and an arc strip (40). The transmission shaft 1 (49) is coaxially rotatably installed in the transmission cavity (103). One end of the transmission shaft 1 (49) is fixedly connected to the transmission assembly (13). The outer wall of the transmission shaft 1 (49) is fixedly connected to one end of the connecting block (48). The other end of the connecting block (48) is fixedly connected to the transmission ring (47). The transmission ring (47) is rotatably connected to the housing (1). The transmission ring (47) ) is fixedly connected to the outer side of one end of a transmission connecting rod (46), and the other end of the transmission connecting rod (46) is fixedly connected to a placement plate (41). The placement plate (41) and the transmission connecting rod (46) are both arranged in a fan-shaped cavity (101). One side edge of the placement plate (41) is fixedly connected to an outer convex strip (43), and the other side edge of the placement plate (41) is fixedly connected to an inner convex strip (45). The end of the inner convex strip (45) opposite to the outer convex strip (43) is fixedly connected to an arc strip (40). An adjustable clamping component (44) is movably provided on one side of the arc strip (40) away from the inner convex strip (45). The two sides between two adjacent placement plates (41) are respectively fixedly connected to connecting strips (42).

6. A cell culture device according to claim 5, characterized in that: Both ends of the outer convex strip (43) and the inner convex strip (45) are respectively provided with slope surfaces, the outer convex strip (43) and the inner convex strip (45) have the same bending direction, and the outer convex strip (43) and the inner convex strip (45) are concentrically arranged.

7. A cell culture device according to claim 6, characterized in that: The adjustable clamping assembly (44) comprises a movable arc strip (441), a threaded rod (442), an internally threaded barrel (443), an adjusting hand wheel (444), and a groove (445); the movable arc strip (441) is slidably arranged on the top surface of the placement plate (41); the middle part of one end of the movable arc strip (441) is fixedly connected to one end of the threaded rod (442); the other end of the threaded rod (442) is sleeved with the internally threaded barrel (443) via a threaded structure; the internally threaded barrel (443) is rotatably connected to the outer convex strip (43); and the end of the internally threaded barrel (443) is fixedly connected to the adjusting hand wheel (444); the adjusting hand wheel (444) is rotatably arranged in the groove (445); and the groove (445) is concavely arranged on the outer convex strip (43).

8. A cell culture device according to claim 7, characterized in that: The transmission assembly (13) comprises an active rotating shaft (131), an active disk (132), an active connecting rod (133), a toggle block (134), a transmission disk (135), and a second transmission rotating shaft (136). The active rotating shaft (131) is rotatably mounted in the cylindrical cavity (102). One end of the active rotating shaft (131) is fixedly connected to a servo motor (7). The active rotating shaft (131) is fixedly connected to one side of the active connecting rod (133). The other side of the active connecting rod (133) is rotatably connected to the toggle block (134). One side of the active rotating shaft (131) is fixedly sleeved on the active disk (132). One side of the active disk (132) slides against the transmission disk (135). The transmission disk (135) is fixedly sleeved on the second transmission rotating shaft (136). One end of the second transmission rotating shaft (136) is fixedly connected to the end of the first transmission rotating shaft (49).

9. A cell culture device according to claim 8, characterized in that: The active connecting rod (133) is arranged between the active disk (132) and the servo motor (7); a plurality of limit openings (1351) are provided on the outer edge of the transmission disk (135); the limit openings (1351) slide against the active disk (132); a toggle opening (1352) is provided between two adjacent limit openings (1351); the toggle opening (1352) is slidably sleeved with the toggle block (134); a fan-shaped opening (1321) is provided on one side of the active disk (132); and the active connecting rod (133) is arranged in the middle of the fan-shaped opening (1321).

10. The cell culture device according to claim 1, characterized in that: The end of the cleaning rotating shaft (9) is fixedly connected with a cleaning rod (91), and a cleaning sponge is bonded to the cleaning rod (91).

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