Preparation method of hemostatic healing-promoting electrostatic spinning wound dressing with Janus structure

Abstract

The invention discloses a hemostatic healing-promoting electrostatic spinning wound dressing with a Janus structure and a preparation method, and belongs to the field of dressing application materials. According to the preparation method, an electrostatic spinning technology is adopted, calcium modified oxidized plant fiber cloth is taken as a receiving substrate, polyvinyl butyral is taken as a spinning forming material, the calcium modified oxidized plant fiber cloth and glycine modified polyethylene glycol are blended, and then electrostatic spinning is performed to prepare the fiber membrane. Amino groups of glycine are condensed with oxidized polyethylene glycol aldehyde groups, and the material is composed of nanofibers, so that the material has a relatively large specific surface area. The carrier material also has good mechanical properties and meets daily sports. The prepared hemostatic dressing can concentrate blood components, the nanofibers effectively gather blood cells, the blood coagulation time is shortened, and meanwhile, the hemostatic dressing can accelerate wound healing.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical dressings, and in particular relates to a Janus structure hemostatic and healing-promoting electrospinning wound dressing and a preparation method thereof. Background Art

[0002] Skin covers the entire human body and is a regulator of temperature and sensory functions. When damaged in emergency situations, such as combat, clinical and car accidents, excessive bleeding caused by ruptured blood vessels is very dangerous, which can lead to hemorrhagic shock and even one of the causes of death. Therefore, it is important to stop bleeding quickly and promote wound healing when an incident occurs. Therefore, hemostatic and healing-promoting wound dressings came into being. The ideal hemostatic and healing-promoting material should have excellent coagulation and absorption capabilities, can quickly aggregate blood cells and platelets, accelerate blood coagulation, absorb wound exudate, and promote cell proliferation, angiogenesis and tissue regeneration.

[0003] Chinese patent CN1109756A discloses a hemostatic agent of calcium-modified oxidized cellulose, characterized in that the oxidized cellulose is modified into a powder with a faster hemostatic effect. Compared with the dosage form, the powder does not provide a wound healing environment compared with a patch combining spinning technology with nanofiber Tencel cloth.

[0004] Chinese patent CN115198511B discloses an oxidized Tencel / carboxyethyl chitosan composite hemostatic Tencel cloth and its preparation method, which is characterized in that a single-layer wound dressing is prepared by cross-linking after blending, and the single-layer fiber membrane cannot discharge wound exudate after absorbing it, and cannot be effectively isolated from the wound, and there is no one-way permeation effect.

[0005] Compared with the above patents, the dosage form is poor; the exudate cannot be effectively isolated from the wound, resulting in slow wound healing. The electrospinning technology is used to prepare a hydrophobic fiber membrane combined with a hydrophilic modified plant fiber cloth to obtain a hemostatic and healing dressing with multiple functions. It has a simple structure, is easy to peel off, has a unidirectional permeation effect, effectively isolates the contact between the wound and the exudate, and has certain mechanical properties. . Summary of the invention

[0006] In order to solve the problems of complex wound dressing manufacturing process, poor dosage form and inability to effectively isolate the wound from contact with exudate, the present invention provides a Janus structured hemostatic and healing-promoting electrospinning wound dressing and a preparation method thereof.

[0007] The inventive point of the present invention is that the patch is made by combining a hydrophobic fiber membrane prepared by electrospinning technology with a hydrophilic calcium-modified plant fiber cloth. The patch is simple to operate, easy to peel off, and has a certain mechanical strength. The modified plant fiber cloth has improved liquid absorption performance and has a unidirectional liquid guiding effect, which isolates the contact between the wound and the exudate and provides a good healing environment.

[0008] The present invention provides a method for preparing a Janus structured hemostatic and healing-promoting electrospun wound dressing, comprising the following steps: (1) Modification of the primary fabric The primary base fabric is subjected to alkalization, etherification, acidification treatment in sequence, and then washed and dried to obtain a modified primary base fabric; (2) Preparation of glycine polyethylene glycol Dissolve 10-20% polyethylene glycol in a pH 9-10 alkaline solution, add 10-20% glycine, react fully, and freeze-dry to obtain glycine polyethylene glycol; (3) Preparation of spinning solution Glycine polyethylene glycol is made into an aqueous solution with a mass concentration of 10-50%, and ultrasonic dispersion and mixing are performed to obtain a glycine polyethylene glycol aqueous solution. Ethanol is added to an ethanol content of 60-95% (v / v), and 2%-10% polyvinyl butyral is added and mixed to obtain a spinning solution; (4) Preparation of electrospinning wound dressings The modified base fabric in step (1) is used as a receiving base, the spinning solution is extracted, and electrospinning is performed at a voltage difference of 10-25 kV, a collection distance of 9-20 cm, and an injection speed of 0.2-1 ml / h to obtain the Janus structured hemostatic and healing electrospun wound dressing.

[0009] Preferably, the base fabric in step (1) is made of: chemical fiber fabric, plant fiber fabric, protein fiber fabric.

[0010] Preferably, the base fabric includes fabrics obtained by oxidation modification or ion modification of chemical fiber fabrics, plant fiber fabrics, or protein fiber fabrics.

[0011] Preferably, in step (1), the base used in the alkalization process is one or more of sodium hydroxide, potassium hydroxide, and calcium hydroxide; the acid used in the acidification process is one or more of acetic acid, sulfuric acid, and phosphoric acid.

[0012] Preferably, a metal salt solution is added during the acidification process.

[0013] Preferably, the molecular weight of the polyethylene glycol in step (2) is between 800 and 10,000.

[0014] Preferably, the needle size used in step (4) is 21-27G.

[0015] The beneficial effects of the present invention are as follows: the preparation method can obtain a loose porous structure; it has the ability to stop bleeding; it has the ability to conduct fluid unidirectionally and has a certain electrical conductivity; and the dressing can serve as a temporary barrier to isolate the environment around the wound.

[0016] The present invention is further described in detail below in conjunction with the examples, but the implementation method of the present invention is not limited thereto.

[0017] Example 1 (1) Preparation of oxidized Tencel cloth 10 grams of primary base fabric was alkalized with 15% sodium hydroxide, etherified with 3.75% chloroacetic acid, and then placed in a tank with 370ml isopropyl alcohol (IPA) and 370ml distilled water, and 0.006mol 10ml acetic acid: 10ml IPA was added. The reaction was stopped after 2 hours. Replace with fresh isopropyl alcohol and stir for another 30 minutes, rinse and dry. Tencel cloth is a kind of plant fiber cloth; (2) Preparation of glycine polyethylene glycol Dissolve 10% polyethylene glycol in a pH 9-10 triethylamine solution, add 10% glycine, fully react for 24 hours, and freeze-dry to obtain glycine polyethylene glycol; (3) Preparation of spinning solution Glycine polyethylene glycol is prepared into a glycine polyethylene glycol aqueous solution with a mass concentration of 40%, and ultrasonic dispersion is performed for 1 hour to mix evenly to obtain a glycine polyethylene glycol aqueous solution. Ethanol is added until the ethanol content is 80% (v / v), and 4% polyvinyl butyral is added, and the mixture is mixed evenly to obtain a spinning solution; (4) Preparation of electrospinning wound dressings The modified Tencel cloth in (1) was used as a receiving substrate, the spinning solution was extracted with a syringe, and a needle was connected. Under the voltage of 15 kV and the collection distance of 12 cm, the injection speed was 0.4 ml / h; electrospinning was performed to obtain a polyvinyl pyrrolidone / glycine modified polyethylene glycol composite modified cloth wound dressing that stops bleeding and promotes healing electrospun fiber wound dressing.

[0018] Example 2 (1) Preparation of calcium-modified oxidized Tencel cloth 10 grams of primary fabric was alkalized with 15% potassium hydroxide, etherified with 3.75% chloroacetic acid, and then placed in 370 ml of isopropyl alcohol (IPA) and 370 ml of distilled water in a tank. 0.38 grams of calcium acetate monohydrate (0.006 mole) was dissolved in 8 ml of distilled water: 8 ml of IPA. The reaction was stopped after 2 hours. Replace with fresh isopropyl alcohol and stir for another 30 minutes. Rinse and dry. The calcium in the fabric sample was analyzed by atomic absorption spectroscopy, and it was found that it had 0.80% (by weight) calcium. Tencel cloth is a kind of plant fiber cloth; (2) Preparation of glycine polyethylene glycol Dissolve 10% polyethylene glycol in a monohydrated ammonia solution at pH 9-10, add 10% glycine, fully react for 24 hours, and freeze-dry to obtain glycine polyethylene glycol; (3) Preparation of spinning solution Glycine polyethylene glycol is prepared into a glycine polyethylene glycol aqueous solution with a mass concentration of 40%, and ultrasonic dispersion is performed for 1 hour to mix evenly to obtain a glycine polyethylene glycol aqueous solution. Ethanol is added until the ethanol content is 80% (v / v), and 4% polyvinyl butyral is added, and the mixture is mixed evenly to obtain a spinning solution; (4) Preparation of electrospinning wound dressings The modified Tencel cloth in (1) was used as a receiving substrate, the spinning solution was extracted with a syringe, and a needle was connected. Under the voltage of 15 kV and the collection distance of 12 cm, the injection speed was 0.4 ml / h; electrospinning was performed to obtain a polyvinyl pyrrolidone / glycine modified polyethylene glycol composite calcium modified Tencel cloth wound dressing that stops bleeding and promotes healing electrospun fiber wound dressing.

[0019] Example 3 (1) Preparation of calcium-modified nonwoven fabrics 10 grams of primary base fabric was alkalized with 15% calcium hydroxide, etherified with 3.75% chloroacetic acid, and then placed in 370 ml isopropyl alcohol (IPA) and 370 ml distilled water in a tank, 0.58 grams of calcium carbonate (0.006 mole) was dissolved in 10 ml distilled water: 10 ml IPA. The reaction was stopped after 2 hours. Fresh isopropyl alcohol was replaced and stirred for another 30 minutes. Rinse and dry. The calcium of the fabric sample was analyzed by atomic absorption spectroscopy, and it was found that it had 1.15% (by weight) calcium. Tencel cloth is a kind of plant fiber cloth; (2) Preparation of glycine polyethylene glycol Dissolve 30% polyethylene glycol in a sodium hydroxide solution at pH 9-10, add 20% glycine, fully react for 24 hours, and freeze-dry to obtain glycine polyethylene glycol; (3) Preparation of spinning solution Glycine polyethylene glycol is prepared into a glycine polyethylene glycol aqueous solution with a mass concentration of 10%, and ultrasonic dispersion is performed for 1 hour to mix evenly to obtain a glycine polyethylene glycol aqueous solution. Ethanol is added until the ethanol content is 95% (v / v), and 8% polyvinyl butyral is added, and the mixture is mixed evenly to obtain a spinning solution; (4) Preparation of electrospinning wound dressings The modified nonwoven fabric in (1) is used as a receiving substrate, the spinning solution is extracted with a syringe, and a needle is connected. Electrospinning is performed at a voltage of 20 kV, a collection distance of 17 cm, and an injection speed of 1 ml / h to obtain a polyvinyl pyrrolidone / glycine modified polyethylene glycol composite calcium modified Tencel cloth wound dressing for hemostasis and healing.

Claims

1. A method for preparing a Janus structure hemostatic and healing-promoting electrospinning wound dressing, characterized in that: The following steps are involved: (1) Modification of the primary fabric The primary base fabric is subjected to alkalization, etherification, acidification treatment in sequence, and then washed and dried to obtain a modified primary base fabric; (2) Preparation of glycine polyethylene glycol Dissolve 10-20% polyethylene glycol in a pH 9-10 alkaline solution, add 10-20% glycine, react fully, and freeze-dry to obtain glycine polyethylene glycol; (3) Preparation of spinning solution Glycine polyethylene glycol is made into an aqueous solution with a mass concentration of 10-50%, and ultrasonic dispersion and mixing are performed to obtain a glycine polyethylene glycol aqueous solution. Ethanol is added to an ethanol content of 60-95% (v / v), and 2%-10% polyvinyl butyral is added and mixed to obtain a spinning solution; (4) Preparation of electrospinning wound dressings The modified base fabric in step (1) is used as a receiving base, the spinning solution is extracted, and electrospinning is performed at a voltage difference of 10-25 kV, a collection distance of 9-20 cm, and an injection speed of 0.2-1 ml / h to obtain the Janus structured hemostatic and healing electrospun wound dressing.

2. The method for preparing a Janus structure hemostatic and healing-promoting electrospinning wound dressing according to claim 1, characterized in that: The base fabric described in (1) is one of chemical fiber fabric, plant fiber fabric and protein fiber fabric.

3. The method for preparing a Janus structure hemostatic and healing-promoting electrospinning wound dressing according to claim 2, characterized in that: The base fabric includes fabrics obtained by oxidation modification or ion modification of chemical fiber fabrics, plant fiber fabrics and protein fiber fabrics.

4. The method for preparing a Janus structure hemostatic and healing-promoting electrospinning wound dressing according to claim 1, characterized in that: In the step (1), the alkali used in the alkalization process is one or more of sodium hydroxide, potassium hydroxide, and calcium hydroxide; the acid used in the acidification process is one or more of acetic acid, sulfuric acid, and phosphoric acid.

5. The method for preparing a Janus structure hemostatic and healing-promoting electrospinning wound dressing according to claim 4, characterized in that: A metal salt solution is added during the acidification process.

6. The method for preparing a Janus structure hemostatic and healing-promoting electrospun wound dressing according to claim 1, characterized in that: The molecular weight of the polyethylene glycol in (2) is between 800 and 10,000.

7. The method for preparing a Janus structure hemostatic and healing-promoting electrospinning wound dressing according to claim 1, characterized in that: The needle size used in (4) is 21~27G.

8. Application of a Janus structured hemostatic and healing-promoting electrospun wound dressing in the preparation of wound dressings.