Method for inducing generation of calculus bovis in ox gall by using special feed

Abstract

The invention discloses a method for inducing generation of calculus bovis in ox gall by using a special feed, and solves the problem of high calculus bovis cultivation cost in the prior art. The method comprises the following steps: S1, selecting healthy old cattle; the aged cattle are six-age cattle with more than four births; s2, 11%-15% of butter fat, 0.8%-1.2% of cholesterol, 0.2%-0.6% of cholic acid, 1.0%-1.8% of corn oil, 35%-45% of cane sugar, 17%-23.0% of casein and 20%-23% of forage are mixed together to be taken by the cattle; s3, when the cattle are fed, 80-150 g / kg of the cattle is fed to each cattle every day, after the cattle are continuously fed for 14-20 days, the cattle are normally fed for 6 months to 1 year, and the cattle are killed and calculus bovis is taken out. According to the method, the cattle are fed with the special feed, so that the cattle generate calculus bovis, the harm to the cattle caused by manual calculus bovis cultivation can be effectively reduced, the yield of the calculus bovis can be stable and can reach 80.7 g at most, the dilemma of shortage of calculus bovis raw materials in China can be effectively relieved, and the direct economic cost of natural calculus bovis is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of bezoar cultivation, and in particular relates to a method for inducing the production of bezoar in cattle gallbladder by utilizing special feed. Background Art

[0002] Bezoar[1] is a gallstone in the gallbladder of cattle, a mammal of the phylum Chordata. The gallstone produced in the gallbladder is called "bile yellow" or "egg yolk", the gallstone produced in the bile duct is called "tube yellow", and the gallstone produced in the hepatic duct is called "liver yellow". The complete bezoar is mostly oval in shape, light in texture, with a golden to yellow-brown surface, delicate and shiny. According to traditional Chinese medicine, bezoar has a light fragrance, a slightly bitter taste followed by sweetness, and is neutral in nature. It can be used to relieve fever, detoxify, and calm nerves. It can be taken internally to treat high fever, coma, insanity, infantile convulsions, convulsions, etc. It can be used externally to treat sore throat, mouth ulcers, carbuncle, and uremia. Natural bezoar is very precious, and its international price is higher than that of gold. Most of the bezoar used is artificial.

[0003] Due to the small amount of natural bezoar, the natural occurrence rate of natural bezoar is less than 0.1%, and the drug source is scarce. my country's annual demand for bezoar is about 5 tons, but the domestic production of natural bezoar is less than 2 tons. The traditional method of cultivating bezoar is generally to implant the drug into the body of the cattle through minimally invasive surgery. Although this method can cultivate bezoar in the body of the cattle, it damages the normal growth of the cattle. At the same time, this method has a high cost when cultivating bezoar. Summary of the invention

[0004] The present invention provides a method for inducing the production of bezoar in cattle gallbladder by using special feed, so as to at least solve part of the above technical problems.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] A method for inducing the production of bezoar in cattle gallbladder by using special feed comprises the following steps:

[0007] S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters;

[0008] S2, mixing butter fat 11%-15%, cholesterol 0.8%-1.2%, bile acid 0.2%-0.6%, corn oil 1.0%-1.8%, sucrose 35%-45%, casein 17%-23.0%, and forage 20%-23% together, and making the cattle take it;

[0009] S3. When feeding cattle, feed 100g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 6 months to 1 year, then kill the cattle and take out the bezoar.

[0010] Further, the following steps are included:

[0011] S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters;

[0012] S2, mix 13.0% butter fat, 1.0% cholesterol, 0.5% bile acid, 1.5% corn oil, 40% sucrose, 20.0% casein, and 23.0% grass, and make the cow take it;

[0013] S3. When feeding cattle, feed 100g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 6 months to 1 year, then kill the cattle and take out the bezoar.

[0014] Further, the following steps are included:

[0015] S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters;

[0016] S2, mix 13.0% butter fat, 1.0% cholesterol, 0.5% bile acid, 1.5% corn oil, 45% sucrose, 20.0% casein, and 17.0% grass, and make the cattle take it;

[0017] S3. When feeding cattle, feed 100g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 1 year, then kill the cattle and take out the bezoar.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The invention has a simple structure, a scientific and reasonable design, and is easy to use. The invention adopts special feed to feed cattle, so that the cattle produce bezoars, which can effectively reduce the damage caused to cattle by humans when cultivating bezoars, and can increase and stabilize the output of bezoars, which can reach up to 80.7 grams at most. It can effectively alleviate the shortage of bezoar raw materials in my country and reduce the direct economic cost of cultivating bezoars.

[0020] The invention greatly shortens the cultivation time of bezoar to 6 months to 1 year, and can effectively reduce the cultivation time of bezoar. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical scheme and advantages of the present invention more clear, the following is further described in detail in conjunction with the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0022] Implementation column 1.

[0023] The present invention provides a method for inducing the production of bezoar in cattle gallbladder by using special feed, comprising the following steps:

[0024] S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters;

[0025] S2, mixing 13.0% butter fat, 1.0% cholesterol, 0.5% bile acid, 1.5% corn oil, 40% sucrose, 20.0% casein, and 23.0% grass together to form a special feed, and making the cattle take it;

[0026] S3. When feeding cattle, feed 100g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 9 months, then kill the cattle and take out the bezoar.

[0027] When selecting healthy aged cattle, the older the better. When feeding, 13.0% butter fat, 1.0% cholesterol, 0.5% bile acid, 1.5% corn oil, 40% sucrose, 20.0% casein and 23.0% grass are mixed together to form special feed. The cattle are weighed and fed according to their weight, that is, once a day, and the weight of each feeding is 80-150g / kg. After continuous feeding for 14 days and normal feeding for 6 hours, the cattle are killed and the bezoars are taken out.

[0028] Implementation column 2.

[0029] A method for inducing the production of bezoar in cattle gallbladder by using special feed comprises the following steps:

[0030] S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters;

[0031] S2, mix 11% butter fat, 0.8% cholesterol, 0.2% bile acid, 1.0% corn oil, 45% sucrose, 22% casein, and 20% grass together, and make the cow take it;

[0032] S3. When feeding cattle, feed 150g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 8 months, then kill the cattle and take out the bezoar.

[0033] Example 3.

[0034] A method for inducing the production of bezoar in cattle gallbladder by using special feed comprises the following steps:

[0035] S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters;

[0036] S2, mix 15% butter fat, 1.2% cholesterol, 0.6% bile acid, 1.2% corn oil, 42% sucrose, 20% casein, and 20% grass, and make the cow take it;

[0037] S3. When feeding cattle, feed 130g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 1 year, then kill the cattle and take out the bezoar.

[0038] Comparative example 1.

[0039] The present invention is the same as Example 1 except that butter fat is 10%, cholesterol is 0.7%, bile acid is 0.1%, corn oil is 0.9%, sucrose is 53.3%, casein is 16%, and forage is 19%.

[0040] Comparative example 2.

[0041] The present invention is the same as Example 1 except that butter fat 16%, cholesterol 1.5%, bile acid 8%, corn oil 2.5%, sucrose 23%, casein 24%, and forage 25%;

[0042] Comparative example 3.

[0043] The present invention is the same as Example 1 except that young cattle are selected.

[0044] Experiment 1.

[0045] The bezoar obtained in Example 1 of the present invention, natural bezoar, and bile acid reference substance were subjected to bile acid thin layer chromatography identification experiments, and it was found that the bezoar obtained in Example 1 and the natural bezoar showed fluorescent spots of the same color at the positions corresponding to the bile acid of the reference substance.

[0046] Experiment 2.

[0047] The bezoar obtained in Example 1 of the present invention, the natural bezoar and the bilirubin reference substance were subjected to a bilirubin thin layer chromatography identification experiment, and it was found that the bezoar obtained in Example 1 of the present invention and the natural bezoar showed fluorescent spots of the same color as the corresponding positions of the reference substance bilirubin.

[0048] Experiment 3.

[0049] One portion of each of the bezoars obtained in Examples 1-3 and three portions of natural bezoars were taken as control samples, and the bilirubin and bile acid contents were measured, as shown in Table 1 below.

[0050] Table 1 Bilirubin and bile acid content

[0051] sample Bilirubin (%) cholic acid(%) Example 1 66.53 14.50 Example 2 66.45 14.58 Example 3 66.64 14.55 Natural bezoar 1 46.71 12.79 Natural bezoar 2 42.38 13.20 Natural Bezoar 3 45.67 12.66

[0052] It can be seen from Table 1 that the bilirubin content of the bezoar obtained by the present invention is above 65%, and the bile acid content is above 14%. The bilirubin and bile acid content of the bezoar cultivated by the present invention and the natural bezoar are measured, and the results are in line with the provisions of the 2020 edition of the Pharmacopoeia. The bilirubin and bile acid contents of the cultivated bezoar of the present invention are higher than those of natural bezoar, and the content is more uniform. Since natural bezoar is obtained by chance, the time difference between the existence of bezoar in the cattle body is relatively large, so the quality is also slightly different.

[0053] Experiment 4.

[0054] This experiment is divided into

[0055] Group 1: 10 aged cows were selected and fed in the manner of Example 1;

[0056] The second group: 10 aged cows were selected and fed in the manner of Example 2;

[0057] The third group: 10 aged cows were selected and fed in the manner of Example 3;

[0058] Group 4: 10 aged cows were selected and fed in the manner of Comparative Example 1;

[0059] Group 5: 10 aged cows were selected and fed in the manner of Comparative Example 2;

[0060] Group 6: 10 aged cows were selected and fed in the manner of Comparative Example 3;

[0061] Group 7: Another 10 cows were selected as the control group for the experiment. After feeding for 3 months, one cow was randomly selected from each group for routine blood test, and the bezoar yield, bilirubin content and bile acid content of the bezoar were measured when the bezoar was taken. The results are shown in Table 2-Table 3, which are as follows:

[0062] Table 2 Blood routine of cattle fed with special diet for 2 months

[0063]

[0064] It can be seen from Table 2 that in the second month before the yellowing period, there was no obvious change in the total number of red blood cells, white blood cells and white blood cell classification in the blood of the cattle in the control group, the embodiment and the comparative example. Without changing the feeding and management conditions, there was no obvious change in the blood routine conditions compared with the control group, which proves that the drug formula composition and process treatment adopted by the present invention will not affect the body function of the cattle.

[0065] Table 3 Bezoar production table

[0066]

[0067] It can be seen from Table 3 that the cattle in the embodiments and comparative examples all have different amounts of bezoar formed, wherein the highest amount of bezoar produced in embodiment 1 can reach 80.7 g, while the amounts produced in comparative examples 1-3 are 60.4, 67.2 and 68.9 g, respectively, and the amount of bezoar produced is significantly reduced; this indicates that the special feed and the selection of cattle of the present invention are the key to the present invention, and when people are cultivating bezoar, under the same ecological environment and technical conditions, the technical scheme of the present invention is the best.

[0068] Finally, it should be noted that the above embodiments are only preferred embodiments of the present invention to illustrate the technical solutions of the present invention, rather than limiting them, and certainly not limiting the patent scope of the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention. In other words, any changes or modifications made to the main design concept and spirit of the present invention that have no substantive significance, and the technical problems they solve are still consistent with the present invention, should be included in the protection scope of the present invention. In addition, the direct or indirect application of the technical solutions of the present invention in other related technical fields is also included in the patent protection scope of the present invention.

Claims

1. A method for inducing the production of bezoar in cattle gallbladder using special feed, characterized in that: The following steps are involved: S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters; S2, mixing butter fat 11%-15%, cholesterol 0.8%-1.2%, bile acid 0.2%-0.6%, corn oil 1.0%-1.8%, sucrose 35%-45%, casein 17%-23.0%, and forage 20%-23% together, and making the cattle take it; S3. When feeding cattle, feed 80-150g / kg per cow once a day. After continuous feeding for 14-20 days, feed normally for 6 months to 1 year, then kill the cattle and take out the bezoar.

2. A method for inducing the production of bezoar in cattle gallbladder using special feed according to claim 1, characterized in that: The following steps are involved: S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters; S2, mix 13.0% butter fat, 1.0% cholesterol, 0.5% bile acid, 1.5% corn oil, 40% sucrose, 20.0% casein, and 23.0% grass, and make the cow take it; S3. When feeding cattle, feed 100g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 6 months to 1 year, then kill the cattle and take out the bezoar.

3. The method of inducing the production of bezoar in cattle gallbladder by using special feed according to claim 1, characterized in that: The following steps are involved: S1. Select healthy aged cattle; the aged cattle are six-year-old cattle that have given birth to four or more litters; S2, mix 13.0% butter fat, 1.0% cholesterol, 0.5% bile acid, 1.5% corn oil, 45% sucrose, 20.0% casein, and 17.0% grass, and make the cow take it; S3. When feeding cattle, feed 120g / kg per cow once a day. After feeding continuously for 14-20 days, feed normally for 1 year, then kill the cattle and take out the bezoar.

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