Soothing, repairing and anti-aging essence and preparation method thereof

Abstract

The invention discloses soothing, repairing and anti-aging essence and a preparation method thereof. The soothing, repairing and anti-aging essence is prepared from the following raw materials: hydrolyzed sclerotium gum, a plant fermentation composition, recombinant III-type humanized collagen and auxiliary materials, the plant fermentation composition comprises the following raw materials: apples, lily rhizomes, Chinese wolfberry fruits and zymophyte, wherein the zymophyte is at least one of lactobacillus plantarum, bifidobacterium, lactobacillus buchneri, yeast, aspergillus niger, aspergillus oryzae and lactobacillus rhamnosus. All the components in the raw materials have a synergistic effect, so that the essence has the effects of resisting oxidation, repairing skin barriers, preventing collagen loss, promoting elastin fiber generation and the like. More importantly, the raw materials have the effect of improving the microbial flora of the facial skin, so that the microbial flora of the facial skin is balanced, and the effects of relieving, repairing and resisting aging are achieved.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to a soothing, repairing and anti-aging essence and a preparation method thereof. Background Art

[0002] With people's growing pursuit of health and the rapid development of dermatology, medical skincare products with higher safety and effectiveness have become an unstoppable trend. As more women begin to prioritize the quality and safety of skincare products, professionals are deepening their understanding of the structure and physiological functions of the skin and its appendages, leading to more specialized research on cosmetics, resulting in more targeted, reliable, and competitive products.

[0003] Mainstream skincare products are primarily developed for moisturizing, soothing, repairing, whitening, and anti-aging. The soothing properties of skincare products can alleviate or eliminate discomfort, irritation, and sensitivity caused by various internal and external factors, restoring the skin to a relatively calm, comfortable, and stable state. The repair properties can repair damaged skin barriers, enhancing the skin's own barrier repair capabilities and protecting the skin. The anti-aging properties can achieve anti-aging effects by reducing collagen breakdown and promoting elastin fiber production.

[0004] Prior art CN115400065B is a kind of skin care product composition with moisturizing, anti-wrinkle, anti-aging, soothing, repairing multi-functional and its application, raw materials include bosaiyin, glycerol glucoside, soluble collagen, beta-alanyl hydroxyprolyl diaminobutyric acid benzylamine, peptides, inositol, ceramide, ergothioneine, sea fennel extract, tulip flower extract and mussel extract and other raw materials. With age, skin microecology is also changing, and the secondary metabolism of flora increases, thereby affecting the normal physiological function of the skin (moisture, firmness, elasticity), and there is damage to the epidermal barrier, and the phenomena such as dermal layer collapse. The above patent does not involve the relevant research on anti-aging from the perspective of facial microecology. Summary of the Invention

[0005] In view of this, the present invention proposes a method for preparing a soothing, repairing and anti-aging essence to solve the above problems.

[0006] A soothing, repairing and anti-aging essence, the raw materials of which include hydrolyzed sclerotium gum, a plant fermentation composition, recombinant type III humanized collagen and auxiliary materials; the raw materials of the plant fermentation composition include apples, lily rhizomes, wolfberries and fermentation bacteria, and the fermentation bacteria are at least one of Lactobacillus plantarum, Bifidobacterium, Lactobacillus buchneri, yeast, Aspergillus niger, Aspergillus oryzae and Lactobacillus rhamnosus.

[0007] Furthermore, the raw materials include, by weight percentage, 0.01-0.5% of hydrolyzed sclerotium gum, 1-10% of plant fermentation composition, 0.01-5% of recombinant type III humanized collagen, and the remainder of auxiliary materials.

[0008] Furthermore, the raw materials of the plant fermentation composition include, by weight, 20-40 parts of apples, 15-30 parts of lily rhizomes, 10-15 parts of wolfberries, 0.01-1 parts of fermentation bacteria, and 40-100 parts of water.

[0009] Furthermore, the fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:10-20.

[0010] Furthermore, the deposit number of Lactobacillus buchneri is GDMCC NO.1.1926; the deposit number of Lactobacillus rhamnosus is GDMCC NO.1.1798; and the above strains were purchased from Guangdong Provincial Microbiological Culture Collection Center.

[0011] Furthermore, the preparation method of the plant fermentation composition comprises the following steps:

[0012] (1) freeze-drying apples, lily rhizomes, and wolfberries and then crushing them to prepare a mixed powder;

[0013] (2) adding water to the mixed powder, heating to 30-45°C, adding fermentation bacteria, and fermenting for 2-20 hours to obtain a fermentation liquid;

[0014] (3) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain a plant fermentation composition.

[0015] Furthermore, the auxiliary material is at least one of a moisturizer, an emulsifier, a preservative, and water.

[0016] Furthermore, the moisturizing agent is at least one of glycerin, mannitol, sodium alginate, isopropyl myristate, allantoin, coconut oil, olive oil, and betaine.

[0017] Furthermore, the emulsifier is at least one of polydimethylsiloxane, PEG-100 stearate, hexyl laurate, cyclopentasiloxane, and lecithin.

[0018] Furthermore, the preservative is at least one of p-hydroxyacetophenone, hexylene glycol, pentylene glycol, potassium sorbate, and sodium dehydroacetate.

[0019] Furthermore, the raw materials include, by weight percentage, 0.01-0.5% of hydrolyzed sclerotium gum, 1-10% of plant fermentation composition, 0.01-5% of recombinant type III humanized collagen, 1-10% of moisturizer, 0.1-2% of emulsifier, 0.01-0.5% of preservative and the balance of water.

[0020] Furthermore, the moisturizing agent includes 20-40 parts of glycerin, 5-10 parts of isopropyl myristate, 3-10 parts of allantoin, and 1-5 parts of sodium alginate, calculated by weight.

[0021] Furthermore, the emulsifier includes 7-10 parts of PEG-100 stearate and 3-5 parts of lecithin by weight.

[0022] Furthermore, the preservative is p-hydroxyacetophenone.

[0023] Furthermore, the amount of the p-hydroxyacetophenone is 0.01-0.05% by mass of the raw materials.

[0024] Furthermore, the preparation method of the soothing, repairing and anti-aging essence comprises mixing the raw materials to obtain a finished product.

[0025] Furthermore, the preparation method of the soothing, repairing and anti-aging essence comprises dissolving the raw materials in water, adding an emulsifier for emulsification, and mixing to obtain the finished product, wherein the dissolution temperature and the emulsification temperature are both 70-90°C.

[0026] The beneficial effects of the present invention are:

[0027] The present invention discloses a soothing, repairing, and anti-aging essence, the raw materials of which include hydrolyzed sclerotium gum, a plant fermentation composition, recombinant humanized type III collagen, and auxiliary materials. The plant fermentation composition is made from apples, lily rhizomes, and wolfberries, and is fermented with Lactobacillus buchneri and Lactobacillus rhamnosus. The synergistic effect of the various components in the raw materials gives the essence the effects of anti-oxidation, repairing the skin barrier, preventing collagen loss, and promoting elastin fiber production. More importantly, the raw materials of the present invention have the effect of improving the facial skin flora, balancing the microbial flora, and achieving soothing, repairing, and anti-aging effects. DETAILED DESCRIPTION

[0028] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0029] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0030] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0031] Green apple extract was purchased from Peptide An Biotechnology Co., Ltd., lily extract was purchased from Guangzhou Meiyi Biotechnology Co., Ltd., and wolfberry extract was purchased from Guangzhou Mingqi Biotechnology Co., Ltd.

[0032] The apples used in the present invention are Australian green apples, which are produced in Shaanxi.

[0033] Example 1

[0034] Preparation method of plant fermentation composition

[0035] (1) Weigh 30 parts of apples, 22 parts of lily rhizomes, 13 parts of wolfberries, 0.5 parts of fermentation bacteria, and 60 parts of water. The fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:15.

[0036] (2) freeze-drying apples, lily rhizomes, and wolfberries and then crushing them to prepare a mixed powder;

[0037] (3) adding water to the mixed powder, heating to 37°C, adjusting the pH to 4.5 with hydrochloric acid, adding fermentation bacteria, and fermenting for 15 hours to obtain a fermentation liquid;

[0038] (4) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain the plant fermentation composition.

[0039] Preparation method of soothing, repairing and anti-aging essence

[0040] (1) According to weight percentage, weigh 0.3% hydrolyzed sclerotium gum, 5% plant fermentation composition, 2% recombinant type III humanized collagen, 10% moisturizer, 0.8% emulsifier, and 0.03% preservative. Among them, according to weight percentage, the moisturizer is 30 parts of glycerin, 8 parts of isopropyl myristate, 7 parts of allantoin, and 3 parts of sodium alginate; the emulsifier is 8.5 parts of PEG-100 stearate and 4 parts of lecithin; and the preservative is p-hydroxyacetophenone.

[0041] (2) Glycerin, isopropyl myristate and an emulsifier were mixed at 80° C. to prepare a mixture 1;

[0042] (3) adding the remaining raw materials into water to prepare mixture 2;

[0043] (4) The mixture 1 and 2 are heated to 80°C to obtain the finished product.

[0044] Example 2

[0045] Preparation method of plant fermentation composition

[0046] (1) Weigh 20 parts of apples, 15 parts of lily rhizomes, 10 parts of wolfberries, 0.01 parts of fermentation bacteria, and 40 parts of water. The fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:10.

[0047] (2) freeze-drying apples, lily rhizomes, and wolfberries and then crushing them to prepare a mixed powder;

[0048] (3) adding water to the mixed powder, heating to 37°C, adjusting the pH to 4.5 with hydrochloric acid, adding fermentation bacteria, and fermenting for 15 hours to obtain a fermentation liquid;

[0049] (4) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain the plant fermentation composition.

[0050] Preparation method of soothing, repairing and anti-aging essence

[0051] (1) According to weight percentage, weigh 0.01% hydrolyzed sclerotium gum, 1% plant fermentation composition, 0.01% recombinant type III humanized collagen, 1% moisturizer, 0.1% emulsifier, and 0.01% preservative. Among them, according to weight percentage, the moisturizer is 20 parts of glycerin, 5 parts of isopropyl myristate, 1 part of allantoin, and 1 part of sodium alginate; the emulsifier is 7 parts of PEG-100 stearate and 1 part of lecithin; and the preservative is p-hydroxyacetophenone.

[0052] (2) Glycerin, isopropyl myristate and an emulsifier were mixed at 80° C. to prepare a mixture 1;

[0053] (3) adding the remaining raw materials into water to prepare mixture 2;

[0054] (4) The mixture 1 and 2 are heated to 80°C to obtain the finished product.

[0055] Example 3

[0056] Preparation method of plant fermentation composition

[0057] (1) Weigh 40 parts of apples, 30 parts of lily rhizomes, 15 parts of wolfberries, 1 part of fermentation bacteria, and 100 parts of water. The fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:20.

[0058] (2) freeze-drying apples, lily rhizomes, and wolfberries and then crushing them to prepare a mixed powder;

[0059] (3) adding water to the mixed powder, heating to 37°C, adjusting the pH to 4.5 with hydrochloric acid, adding fermentation bacteria, and fermenting for 15 hours to obtain a fermentation liquid;

[0060] (4) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain the plant fermentation composition.

[0061] Preparation method of soothing, repairing and anti-aging essence

[0062] (1) According to weight percentage, weigh 0.5% hydrolyzed sclerotium gum, 10% plant fermentation composition, 5% recombinant type III humanized collagen, 10% moisturizer, 2% emulsifier, and 0.05% preservative. Among them, according to weight percentage, the moisturizer is 40 parts of glycerin, 10 parts of isopropyl myristate, 10 parts of allantoin, and 5 parts of sodium alginate; the emulsifier is 10 parts of PEG-100 stearate and 5 parts of lecithin; and the preservative is p-hydroxyacetophenone.

[0063] (2) Glycerin, isopropyl myristate and an emulsifier were mixed at 80° C. to prepare a mixture 1;

[0064] (3) adding the remaining raw materials into water to prepare mixture 2;

[0065] (4) The mixture 1 and 2 are heated to 80°C to obtain the finished product.

[0066] Comparative Example 1

[0067] On the basis of Example 1, the raw materials of the plant fermentation composition were replaced with green apple extract, lily extract and wolfberry extract.

[0068] Comparative Example 2

[0069] On the basis of Example 1, the ratio of fermentation bacteria was adjusted as follows:

[0070] Preparation method of plant fermentation composition

[0071] (1) Weigh 30 parts of apples, 22 parts of lily rhizomes, 13 parts of wolfberries, 0.5 parts of fermentation bacteria, and 60 parts of water. The fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:5.

[0072] (2) freeze-drying apples, lily rhizomes, and wolfberries and then crushing them to prepare a mixed powder;

[0073] (3) adding water to the mixed powder, heating to 37°C, adjusting the pH to 4.5 with hydrochloric acid, adding fermentation bacteria, and fermenting for 15 hours to obtain a fermentation liquid;

[0074] (4) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain the plant fermentation composition.

[0075] Comparative Example 3

[0076] The preparation method of the plant fermentation composition was adjusted based on Example 1; specifically,

[0077] Preparation method of plant fermentation composition

[0078] (1) Weigh 30 parts of apples, 22 parts of lily rhizomes, 13 parts of wolfberries, 0.5 parts of fermentation bacteria, and 60 parts of water. The fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:15.

[0079] (2) Wash and crush apples, lily rhizomes, and wolfberries to prepare a mixture;

[0080] (3) adding water to the mixture, heating it to 37°C, adjusting the pH to 4.5 with hydrochloric acid, adding fermentation bacteria, and fermenting for 15 hours to obtain a fermentation broth;

[0081] (4) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain the plant fermentation composition.

[0082] Test Example 1 Antibacterial Experiment

[0083] The bacterial strains used for the test were Staphylococcus epidermidis, Propionibacterium acnes, and Staphylococcus aureus, which were purchased from Guangdong Provincial Microbial Culture Collection Center.

[0084] Preparation of bacterial solution: PBS buffer was used to prepare the bacterial strains to a concentration of 5×10 6 CFU / mL of bacterial solution.

[0085] The Oxford cup method was used. The experimental procedure involved applying 100 μL of bacterial solution to the surface of the culture medium. The Oxford cup was then placed upside down on the surface of the culture medium. 1% of the sample solution (the sample solutions were the plant fermentation compositions prepared in Example 1 and Comparative Examples 1-3) was added and incubated in a 37°C incubator for 18 hours. The diameter of the inhibition zone was measured using the crosshatch method.

[0086]

[0087] Experimental results show that the plant fermentation composition prepared using apple, lily rhizome, wolfberry, and fermented bacteria has a significant inhibitory effect on Propionibacterium acnes and Staphylococcus aureus, but no inhibitory effect on Staphylococcus epidermidis. Propionibacterium acnes is commonly found in sebaceous glands and commonly causes skin problems such as acne, papules, and pustules. Staphylococcus aureus is a common bacterium that can cause facial redness, pain, itching, and other problems. Staphylococcus epidermidis is a key bacterial species in maintaining skin health. The plant fermentation composition prepared by the present invention inhibits the growth of harmful bacteria such as Propionibacterium acnes and Staphylococcus aureus, thereby balancing the skin microbiome. Comparative Example 1, tested using commercially available raw materials, yielded a finished product with poor inhibitory effect on Propionibacterium acnes and Staphylococcus aureus. Adjusting the dosage of fermented bacteria in Comparative Example 2 resulted in a decrease in the antibacterial effect of the finished product. In Comparative Example 3, the raw materials were not freeze-dried but directly washed and pulverized, resulting in a decrease in the antibacterial effect of the finished product.

[0088] The present invention utilizes apples, lily rhizomes, wolfberries, and fermented bacteria to prepare a plant fermentation composition. First, the freeze-drying followed by pulverization process prevents oxidation of the active ingredients during the pulverization process, as well as moisture loss and other raw material losses. Through the rational ratio of the raw materials, the active ingredients are fully retained during the fermentation process, generating secondary metabolites that help regulate the balance of the skin's surface flora. This results in an essence that achieves repairing, soothing, and anti-aging benefits.

[0089] Test Example 2

[0090] The hyaluronidase inhibition test was conducted according to the test method of QTTZ-XZ-35 "Testing Rules for Verification of Soothing Efficacy of Cosmetics".

[0091] Test samples: The finished products obtained in Example 1 and Comparative Examples 1-3 were diluted with deionized water to prepare samples with a concentration of 5%.

[0092] name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Inhibition rate (%) 77.4 52.9 63.9 71.2

[0093] The experimental results show that the essence prepared by the present invention has a good inhibitory effect on hyaluronidase, indicating that the finished product has a good soothing effect.

[0094] Test Example 3DPPH clearance test

[0095] The finished products and DPPH solutions obtained in Example 1 and Comparative Examples 1-3 were diluted with anhydrous ethanol to prepare solutions with a concentration of 0.2 mg / mL.

[0096] Sample group: Mix the diluted finished product and DPPH solution in a volume ratio of 1:1.

[0097] Blank solvent group: the diluted DPPH solution and anhydrous ethanol were mixed at a volume ratio of 1:1.

[0098] Blank sample group: The diluted finished product and anhydrous ethanol were mixed in a volume ratio of 1:1.

[0099] The mixture was placed in a dark place for 30 min, and the absorbance of each group was detected at 517 nm by spectrophotometry to calculate the DPPH clearance rate.

[0100] Clearance % = [1-(absorbance of sample group - absorbance of blank sample group) / absorbance of blank solvent group] × 100%

[0101] name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Clearance rate (%) 93.5 71.3 85.9 83.7

[0102] Experimental results show that the finished product prepared by the present invention has good antioxidant properties and better anti-aging effect.

[0103] Combined with the analysis of the experimental results of Test Examples 1-3, the rational ratio of the raw materials of the essence of the present invention can improve the soothing, anti-aging, and repairing effects of the finished product.

[0104] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A soothing, repairing and anti-aging essence, characterized in that: The raw materials include hydrolyzed sclerotium gum, a plant fermentation composition, recombinant type III humanized collagen and auxiliary materials; the raw materials of the plant fermentation composition include apples, lily rhizomes, wolfberries and fermentation bacteria, and the fermentation bacteria are at least one of Lactobacillus plantarum, Bifidobacterium, Lactobacillus buchneri, yeast, Aspergillus niger, Aspergillus oryzae and Lactobacillus rhamnosus.

2. The soothing, repairing and anti-aging essence according to claim 1, characterized in that: In terms of weight percentage, the raw materials include 0.01-0.5% of hydrolyzed sclerotium gum, 1-10% of plant fermentation composition, 0.01-5% of recombinant type III humanized collagen, and the remainder of auxiliary materials.

3. The soothing, repairing and anti-aging essence according to claim 1, characterized in that: By weight, the raw materials of the plant fermentation composition include 20-40 parts of apples, 15-30 parts of lily rhizomes, 10-15 parts of wolfberries, 0.01-1 parts of fermentation bacteria and 40-100 parts of water.

4. The soothing, repairing and anti-aging essence according to claim 1, characterized in that: The fermentation bacteria are Lactobacillus buchneri and Lactobacillus rhamnosus in a mass ratio of 1:10-20.

5. The soothing, repairing and anti-aging essence according to claim 1, characterized in that: The preparation method of the plant fermentation composition comprises the following steps: (1) freeze-drying apples, lily rhizomes, and wolfberries, and then crushing them to prepare a mixed powder; (2) Add water to the mixed powder, raise the temperature to 30-45°C, add fermentation bacteria, and ferment for 2-20 hours to obtain a fermentation liquid; (3) Filtering and centrifuging the fermentation liquid, and collecting the supernatant to obtain the plant fermentation composition.

6. The soothing, repairing and anti-aging essence according to claim 1, characterized in that: The auxiliary material is at least one of a moisturizer, an emulsifier, a preservative and water.

7. The soothing, repairing and anti-aging essence according to claim 6, characterized in that: The moisturizing agent is at least one of glycerin, mannitol, sodium alginate, isopropyl myristate, allantoin, coconut oil, olive oil and betaine.

8. The soothing, repairing and anti-aging essence according to claim 6, characterized in that: The emulsifier is at least one of dimethicone, PEG-100 stearate, hexyl laurate, cyclopentasiloxane and lecithin.

9. The soothing, repairing and anti-aging essence according to claim 6, characterized in that: The preservative is at least one of p-hydroxyacetophenone, hexylene glycol, pentylene glycol, potassium sorbate and sodium dehydroacetate.

10. The method for preparing the soothing, repairing and anti-aging essence according to any one of claims 1 to 9, characterized in that: The raw materials are mixed to obtain the finished product.

Images