Phospholipid type NADH anti-aging capsule and preparation method thereof

Abstract

The invention discloses a phospholipid type NADH (nicotinamide adenine dinucleotide) anti-aging capsule and a preparation method thereof, and belongs to the technical field of health food. The anti-aging capsule is prepared by mixing an NADH micro-capsule preparation and an auxiliary agent and then adding the mixture into a capsule shell, the NADH microcapsule preparation is prepared by taking NADH as a capsule core, chlorophyll as a middle layer and a composite material compounded by phospholipid and beewax as a capsule wall to prepare a microcapsule and drying the microcapsule. The anti-aging capsule prepared by the invention is stable in character, not easy to oxidize, high in bioavailability (high conversion rate of NADH) and high in nutritional value.

Description

Technical Field

[0001] The present application belongs to the technical field of health food, and relates to a phospholipid-type NADH anti-aging capsule and a preparation method thereof. Background Art

[0002] NADH participates in thousands of physiological metabolic reactions in the human body and plays an important role in anti-cell aging, mitochondrial repair, and beauty. Sufficient NADH can better protect the body, prevent and control diseases, and in vitro supplementation of NADH is beneficial to enhance metabolism and promote the body's various physiological functions to maintain optimal conditions. However, NADH is a biological form of hydrogen and is extremely unstable. Light, acidic environment, or oxygen and moisture in the air will destroy the molecular structure of NADH. Currently, NADH products on the market are basically in the form of hard capsules filled with powder or tablets. During the preparation or storage of the product, NADH is easily oxidized and decomposed, and its absorption and utilization rate needs to be improved.

[0003] Among them, NADH capsule products are basically NADH dissolved in vegetable oil and then filled into capsules. Vegetable oil is easily oxidized in the air or at a high temperature, resulting in rancidity, which will reduce its quality and nutritional value. It is also difficult to ensure its activity during the preparation process; and the bioavailability of the product is not high. In addition, patent CN110339179 discloses a method for preparing NADH capsules: stirring and heating edible vegetable oil or edible alcohol to melt, cooling, and then stirring and mixing NADH or NADPH with the edible vegetable oil or edible alcohol to obtain a raw material mixture after grinding and sieving; stirring and mixing water and capsule shell molding raw materials to obtain a colorless glue; using a viscometer to detect that the viscosity of the glue reaches 10000mPa.s or more, the glue is cooled; the glue after cooling is sieved; the raw material mixture and the glue after sieving are used to prepare soft capsules with a capsule machine, and the mixture is shaped and dried. Its NADH absorption and utilization is low, and it is easy to oxidize and decompose during storage, making it difficult to ensure its activity; and the ingredients are single, and the nutritional value needs to be improved.

[0004] Therefore, there is a need to prepare a NADH soft capsule with stable properties, not easily oxidized, high bioavailability (high NADH conversion rate) and high nutritional value, which is also a difficulty in current research. Summary of the invention

[0005] In order to solve the above problems, a phospholipid-type NADH anti-aging capsule and a preparation method thereof are provided. The anti-aging capsule prepared in the present application has stable properties, is not easily oxidized, has high bioavailability (high NADH conversion rate), and has high nutritional value.

[0006] The specific technical solutions of this application are as follows:

[0007] The present application provides a phospholipid-type NADH anti-aging capsule, which is prepared by mixing a NADH microcapsule preparation and an auxiliary agent and then adding the mixture to a capsule shell;

[0008] The NADH microcapsule preparation is prepared by using NADH as the capsule core, chlorophyll as the middle layer, and a composite material of phospholipid and beeswax as the capsule wall to form a microcapsule, which is then dried.

[0009] Optionally, the microcapsule preparation comprises the following components by weight:

[0010] NADH 5-15 parts, chlorophyll 5-15 parts, phospholipids 10-50 parts, beeswax 5-50 parts; by mass, the amount of the additive added is 1.5-2.5 times the mass of the microcapsule preparation.

[0011] Optionally, the beeswax is obtained by compounding white beeswax and yellow beeswax; and the phospholipid is selected from soybean lecithin and sunflower lecithin.

[0012] Optionally, the beeswax is obtained by compounding white beeswax and yellow beeswax in a mass ratio of (1-3): (3-4); and the phospholipid is obtained by compounding soybean lecithin and sunflower lecithin in a mass ratio of (1-3): (4-6).

[0013] Optionally, the auxiliary agent is selected from biological ribose, coenzyme Q10, soy lecithin, vitamins and minerals; the vitamins and minerals are selected from calcium, iron, zinc, selenium, copper, manganese, VC, VE, VB3, VB1, and VB2.

[0014] Optionally, the auxiliary agent is obtained by compounding biological ribose: coenzyme Q10: soybean lecithin: vitamins and minerals according to the mass ratio of (60-80): (45-50): (95-100): (30-33).

[0015] Optionally, the capsule shell is a hard capsule, including HPMC hydroxypropyl methylcellulose empty capsules and gelatin empty capsules.

[0016] The present application also provides a method for preparing the above-mentioned phospholipid-type NADH anti-aging capsule, comprising:

[0017] Step 1: Take chlorophyll and place it in an ethanol solution to prepare a chlorophyll solution; pour it into a batching tank, introduce nitrogen to exhaust the air in the tank, add NADH and mix thoroughly to obtain a mixed solution, perform water bath ultrasound to completely dissolve NADH, filter, wash, and dry to obtain chlorophyll-encapsulated NADH lipid microcapsules;

[0018] Step 2: heating beeswax, phospholipids and ethanol in a water bath and stirring until the beeswax and phospholipids are dissolved to obtain a beeswax-phospholipid complex; stirring and mixing the chlorophyll-coated NADH lipid microcapsules and the beeswax-phospholipid complex under vacuum stirring conditions; homogenizing and then spray drying to obtain a NADH microcapsule preparation;

[0019] Step 3: Evenly mix the above NADH microcapsule preparation and the auxiliary agent to obtain a NADH microcapsule mixture, and fill the mixture into hard capsules to obtain a NADH capsule product.

[0020] Optionally, the water bath ultrasonication conditions in step 1 are about 25-28° C., ultrasonic frequency: 30-40 kHz, and water bath ultrasonication time: 12-25 min;

[0021] The conditions for water bath heating and stirring in step 3 are: temperature 30-40° C., stirring speed 70-110 rpm.

[0022] The present application also provides the use of the above-mentioned phospholipid-type NADH anti-aging capsule in the preparation of anti-aging drugs.

[0023] The beneficial effects of this application include but are not limited to:

[0024] 1. The application adds chlorophyll to wrap the capsule core NADH, which can not only prevent NADH oxidation, but also increase the conversion rate of NADH in cells and promote its absorption by human cells; achieving effects that other antioxidants cannot achieve. Further, the use of a complex of beeswax and phospholipids as a wall material to wrap the chlorophyll with NADH as the capsule core can isolate the air to inhibit the degradation of chlorophyll; at the same time, the compounding of specific beeswax and specific phospholipids can further promote the conversion and absorption of NADH in cells.

[0025] 2. The application adds specific auxiliary agents to improve the nutritional value of the capsules. At the same time, the NADH microcapsule preparation obtained by compounding specific beeswax and specific phospholipids can be better mixed with the auxiliary agents.

[0026] 3. Furthermore, by optimizing the ratio between the auxiliary agents, the conversion and absorption efficiency of NADH in cells and the effect of the capsule in removing free radicals such as DPPH can be further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0028] Figure 1 This is a comparison chart of the therapeutic effect of the phospholipid NADH capsule of the present application before and after taking it;

[0029] Figure 2 This is a comparison chart of the effects of the phospholipid NADH capsule in this application on treating memory and attention before and after taking it. DETAILED DESCRIPTION

[0030] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0031] Unless otherwise specified, the raw materials in the examples of the present application are purchased through commercial channels; in addition, the weight parts of the components in the following embodiments can be replaced by weight units such as "gram", "kilogram" and "jin" in equal proportion.

[0032] Example 1

[0033] A phospholipid-type NADH anti-aging capsule, wherein the anti-aging capsule is prepared by mixing a NADH microcapsule preparation and an auxiliary agent and then adding the mixture into a capsule shell;

[0034] The NADH microcapsule preparation is prepared by using 15 parts of NADH as the capsule core material, 5 parts of chlorophyll (chlorophyll powder purchased from Guangzhou Weilun Food Co., Ltd.) as the middle layer, and a composite material of 10 parts of phospholipids and 50 parts of beeswax as the capsule wall material. The microcapsule preparation is prepared after drying. The obtained microcapsule preparation is in granular form with a particle size of 100 μm.

[0035] The auxiliary agent is obtained by compounding biological ribose, coenzyme Q10, soybean lecithin, vitamins and minerals.

[0036] The NADH microcapsule preparation is specifically prepared by the following method:

[0037] Step 1: Take chlorophyll and place it in an ethanol solution (according to the volume ratio, the ethanol solution is 1% of the volume of chlorophyll) to prepare a chlorophyll solution; pour it into a batching tank, introduce nitrogen to exhaust the air in the tank, add NADH and mix well to obtain a mixed solution, and perform water bath ultrasound (water bath temperature: 25°C, ultrasound frequency: 40kHz) 。 Water bath ultrasonication time: 20 min) to completely dissolve NADH, filter with 1 μm microporous membrane, wash, and dry (35°C, as long as it is lower than 40°C) to obtain chlorophyll-encapsulated NADH lipid microcapsules;

[0038] Step 2, beeswax (white beeswax: yellow beeswax are compounded in a mass ratio of 1:1) and phospholipids (soybean lecithin: sunflower phospholipids are compounded in a mass ratio of 1:2) and a small amount of ethanol (the g / mL mass volume ratio to the total weight of beeswax and phospholipids is 1:5) are placed in a water bath and heated in a water bath at 40°C and 100 rpm until the beeswax and phospholipids are dissolved to obtain a beeswax-phospholipid complex, and the chlorophyll-coated NADH liposome microcapsules and the beeswax-phospholipid complex are stirred and mixed under vacuum stirring conditions, and the NADH microcapsule preparation is obtained by spray drying after homogenization for 15 minutes;

[0039] Step 3: Add biological ribose (D-ribose), coenzyme Q10, soy lecithin, vitamins and minerals (by mass ratio, iron: VC: VE: VB1: VB2: VB3 = 5: 25: 22: 3: 3.5: 13.5) to the NADH microcapsule preparation prepared above and mix evenly. By mass ratio, biological ribose (D-ribose): coenzyme Q10: soy lecithin: vitamins and minerals are 100: 75: 50: 100: 30, respectively; obtain the NADH microcapsule mixture, and fill it into a hard capsule shell (HPMC hydroxypropyl methylcellulose hollow capsule shell) to obtain a NADH capsule product.

[0040] Among them, in step three, soybean lecithin is added during capsule canning to promote the absorption of other auxiliary components in the human body and also have the effect of relieving fatigue.

[0041] Example 2 A phospholipid type NADH anti-aging capsule, beeswax is screened on the basis of Example 1, and the rest is the same as Example 1.

[0042] NADH is also sensitive to light, and light will accelerate its decomposition, affecting its activity and efficacy. Therefore, the effects of different beeswaxes on the quality of microgel preparations were tested under light conditions, and the results are shown in Table 1 below.

[0043] The capsule preparations prepared by adding different beeswax (i.e., when not added into the hard capsule shell at this time, 500 mg; the operation is performed within 24 hours after preparation, the same as in Example 3 below) are sequentially referred to as treatment groups 1-8, as shown in Table 1, and are subjected to light treatment (continuous light treatment for 15 days in a sealed glass dish, light intensity of 3500 lux, temperature of 15°C). The control group is the capsule preparation of treatment group 1 stored in a dark and sealed manner.

[0044] The WST-8 method was used to detect the conversion of NADH in the samples of different treatment groups and the control group in Table 1.

[0045] Experimental procedure: The capsule preparations (500 mg) in treatment groups 1-8 and the control group were placed in a GES1 (immortalized gastric mucosal epithelial cell line) culture dish with a cell number of 3 million. After the culture dish was incubated in a cell culture incubator for 24 hours, the NADH concentration and NAD+ conversion content in the cells were detected.

[0046] According to WST-8 method:

[0047] a. Determination of the total amount of NAD+ and NADH: Ethanol is oxidized to acetaldehyde by alcohol dehydrogenase (ADH), and NAD+ is reduced to NADH in this reaction process; the generated NADH reduces WST-8 to orange-yellow formazan by the action of the electron coupling reagent 1-mPMS (1-Methoxy-5-methylphenazinium MethylSulfate), with a maximum absorption peak at around 450nm. The formazan generated in the reaction system is proportional to the total amount of NAD+ and NADH in the sample.

[0048] b. Determine the amount of NADH alone: ​​After heating in a 60℃ water bath for 30 minutes, the NAD+ in the sample will decompose and only NADH will remain. NADH reduces WST-8 to formazan, and the amount of formazan generated by the reaction is determined by colorimetry, and ultimately the amount of NADH in the sample can be determined.

[0049] c. Determination of NAD+ and NAD+ / NADH ratio: Based on the total amount of NAD+ and NADH and the amount of NADH obtained in the first two steps, the amount of NAD+ in the sample and the ratio of NAD+ / NADH can be calculated.

[0050] Table 1 Effects of different beeswax on microcapsule preparations

[0051]

[0052]

[0053] According to the data in the above table, when the beeswax obtained by compounding white beeswax and yellow beeswax in a mass ratio of (1-3): (3-4) is mixed with other components, the texture is most suitable, the mixing effect is best, and the quality of the anti-aging capsules obtained is relatively the best.

[0054] When white beeswax and yellow beeswax are compounded in a mass ratio of 1:4, the beeswax combination has the best effect; the beeswax combination obtained at this time has the best texture and the best mixing effect with other components; it can further improve the NADH encapsulation rate in the microcapsule preparation and the conversion rate of NADHH into NAD+ after entering the cell, and can also inhibit the decomposition rate of NADH under light conditions.

[0055] The reason may be that polar groups such as hydroxyl groups in white beeswax and yellow beeswax form hydrogen bonds with certain polar sites in the NADH molecule. The formation of this hydrogen bond will affect the spatial conformation of NADH to a certain extent. When the hydrogen bond effect is strong (the chemical bond structure inside white beeswax is more simple and pure, and it shows higher stability and consistency in physical and chemical properties. Therefore, there is a certain relationship between the strength of hydrogen bonding and the addition ratio of white beeswax and yellow beeswax), the molecular structure of NADH will become more stable, reducing the exposure of its active sites, thereby inhibiting the ability of NADH molecules to produce active free radicals under light conditions and react with surrounding molecules to a certain extent.

[0056] It is not easy for a person skilled in the art to improve the NADH embedding rate, NADH conversion rate and light-resistant decomposition rate by selecting a specific beeswax combination. Based on this, the next step of the experiment was carried out.

[0057] Example 3 A phospholipid type NADH anti-aging capsule, based on the optimal basis of Example 2 (the beeswax is obtained by compounding white beeswax and yellow beeswax in a mass ratio of 1:4), the composite phospholipids are screened, and the rest is the same as Example 1.

[0058] The capsule preparations prepared by adding different phospholipids (500 mg, not added into the hard capsule shell at this time) were sequentially designated as treatment groups 1-7, as shown in Table 2, and were subjected to light treatment (continuous light exposure for 15 days in a sealed glass dish, light intensity of 3500 lux, temperature of 15°C). The control group was the capsule preparation of treatment group 1 sealed and stored in the dark.

[0059] The WST-8 method was used to detect the conversion of NADH in the samples of the different treatment groups in the table below. The specific steps are the same as above.

[0060] Table 2 Effects of different phospholipids on microgel preparations

[0061]

[0062]

[0063] According to the above table data, when the combined phospholipids prepared by the mass ratio of soybean lecithin: sunflower lecithin (1-3): (4-6) are applied to the present application, the technical effect is the best, among which the NADH embedding rate and NADH conversion effect in the cell after the compounding of soybean lecithin: sunflower lecithin in the mass ratio of 1:4 are the best; the reason may be that soybean lecithin and sunflower lecithin can play the best synergistic role at this time: the fatty acid chains in the phospholipid molecules have strong hydrophobicity, and the NADH inserted into the phospholipid bilayer membrane is protected by the membrane environment, reducing its risk of oxidation or degradation. Compared with soybean lecithin and sunflower lecithin, the fatty acid composition and polar head group structure in egg yolk lecithin are different, which affects the nature of the chemical bond (the sphingosine in the polar head group of egg yolk lecithin contains sphingomyelin, which affects the biological activity of egg yolk lecithin). The next step of the experiment is based on this.

[0064] Example 4: Phospholipid-type NADH anti-aging capsules prepared under the above-mentioned preferred conditions.

[0065] A phospholipid-type NADH anti-aging capsule, wherein the anti-aging capsule is prepared by mixing a NADH microcapsule preparation and an auxiliary agent and then adding the mixture into a capsule shell;

[0066] In parts by mass, the NADH microcapsule preparation is prepared by using 5 parts of NADH as the capsule core material, 15 parts of chlorophyll (chlorophyll powder purchased from Guangzhou Weilun Food Co., Ltd.) as the middle layer, and a composite material of 50 parts of phospholipids and 5 parts of beeswax as the capsule wall material, and then drying it to obtain the microcapsule.

[0067] The amount of the auxiliary agent added is 1.5 times the mass of the microcapsule preparation.

[0068] The auxiliary agent is prepared from biological ribose, coenzyme Q10, soybean lecithin, vitamins and minerals.

[0069] Specifically prepared by the following method:

[0070] Step 1: Take chlorophyll and place it in an ethanol solution (according to the volume ratio, the ethanol solution is 1% of the volume of chlorophyll) to prepare a chlorophyll solution; pour it into a batching tank, introduce nitrogen to exhaust the air in the tank, add NADH and mix well to obtain a mixed solution, and perform water bath ultrasound (water bath temperature: 28°C, ultrasound frequency: 30kHz) 。 Ultrasonication time: 12 min) to completely dissolve NADH, filter with 1 μm microporous membrane, wash, and dry (37°C) to obtain chlorophyll-encapsulated NADH lipid microcapsules;

[0071] Step 2, beeswax (white beeswax: yellow beeswax are compounded in a mass ratio of 1:4) and phospholipids (soybean lecithin: sunflower phospholipids are compounded in a mass ratio of 1:4) and a small amount of ethanol (the amount added is the same as in Example 1) are placed in a water bath and heated in a water bath at 40°C and 100 rpm until the beeswax and phospholipids are dissolved to obtain a beeswax-phospholipid complex, and the chlorophyll-coated NADH liposome microcapsules and the beeswax-phospholipid complex are stirred and mixed under vacuum stirring conditions, homogenized for 15 minutes, and then spray-dried to obtain a NADH microcapsule preparation;

[0072] Step 3: Add biological ribose (D-ribose), coenzyme Q10, soy lecithin, vitamins and minerals (by mass ratio, iron: VC: VE: VB1: VB2: VB3 = 5: 25: 22: 3: 3.5: 13.5) to the NADH microcapsule preparation prepared above, and the mass ratio is 100: 75: 50: 100: 30, respectively; mix evenly to obtain a NADH microcapsule mixture, and pour it into hard capsules (HPMC hydroxypropyl methylcellulose hollow capsules) to obtain a NADH capsule product.

[0073] The difference between Example 5 and Example 4 is that:

[0074] The water bath ultrasonic conditions in step 1 are: water bath temperature: 25° C., ultrasonic frequency: 40 kHz, water bath ultrasonic time: 25 min, and drying temperature: 32° C. The rest is the same as in Example 4.

[0075] Example 6

[0076] The difference from Example 4 is that the microcapsule preparation is prepared from the following components by weight: 15 parts of NADH, 8 parts of chlorophyll, 15 parts of phospholipids, and 20 parts of beeswax. The rest is the same as Example 1.

[0077] Example 7

[0078] The difference from Example 4 is that the microcapsule preparation is prepared from the following components by weight: 20 parts of NADH, 5 parts of chlorophyll, 8 parts of phospholipids, and 55 parts of beeswax. The rest is the same as Example 1.

[0079] Example 8

[0080] The difference from Example 4 is that the added auxiliary agent is different: lipoic acid: coenzyme Q10: vitamins and minerals are prepared according to a mass ratio of 60:50:35. The vitamins and minerals and other conditions are the same as in Example 1.

[0081] Example 9

[0082] The difference from Example 4 is that the addition ratio of the auxiliary agent is different: the auxiliary agent is obtained by compounding biological ribose: coenzyme Q10: soybean lecithin: vitamins and minerals in a mass ratio of 85:50:70:30.

[0083] Comparative Example 1

[0084] The difference from Example 4 is that chlorophyll is replaced by anthocyanin. The rest is the same as Example 1.

[0085] Comparative Example 2

[0086] The difference from Example 4 is that beeswax is replaced with artificial fruit wax (shellac wax: palm wax is compounded in a mass ratio of 1:1). The rest is the same as Example 1.

[0087] Comparative Example 3

[0088] The difference from Example 4 is that no chlorophyll is added, and the rest is the same as Example 1.

[0089] Comparative Example 4

[0090] The difference from Example 4 is that beeswax is not added, and the rest is the same as Example 1.

[0091] Test Example 1: The performance of the anti-aging capsules prepared in Examples 4-9 and Comparative Examples above was tested. The results are shown in the following table:

[0092] The detection method of NADH is the same as above;

[0093] Ability to scavenge DPPH free radicals:

[0094] Prepare dilution solutions of microcapsule preparation samples of different concentrations (diluted 100, 50, 25, 10, and 1 times to prepare gradient dilution solutions with concentrations of 10 μg / mL, 20 μg / mL, 40 μg / mL, 100 μg / mL, and 500 μg / mL); take 150 μL of the above-mentioned sample and 150 μL of 60 mg / L DPPH solution (diluted with anhydrous ethanol) in a 96-well plate, mix well, store at 30°C in the dark, and measure the absorbance at 519 nm using an enzyme reader after 30 minutes.

[0095] DPPH free radical scavenging rate = [(A blank - A sample) / A blank] × 100%.

[0096] A blank is the absorbance of 150 μL deionized water and 150 μL DPPH solution, and A sample is the absorbance of 150 μL sample and 150 μL DPPH solution.

[0097] Ability to scavenge ABTS free radicals:

[0098] (1) Prepare dilution solutions of microcapsule preparation samples of different concentrations (dilute 100, 50, 25, 10, 1 times to prepare gradient dilution solutions with concentrations of 10 ug / mL, 20 ug / mL, 40 ug / mL, 100 ug / mL, and 500 ug / mL).

[0099] (2) Mix 7 mmol / L ABTS solution and 140 mmol / L potassium persulfate solution in a ratio of 62.5:1 and let stand overnight at room temperature in the dark to form an ABTS stock solution. Dilute it with deionized water to a 5 mmol / L ABTS working solution before use.

[0100] (3) Take 0.15 mL of the above dilution sample and mix it with 2.85 mL of ABTS working solution, let it stand at 30°C for 8 min, and measure the absorbance at 734 nm using deionized water as blank.

[0101] ABTS free radical scavenging rate = [(A blank - A sample) / A blank] × 100%.

[0102] Where A blank is the absorbance of 0.15 mL deionized water and 2.85 mL ABTS working solution, and A sample is the absorbance of 0.15 mL sample and 2.85 mL ABTS working solution.

[0103] Table 3 Performance test of anti-aging capsules stored at room temperature (25°C) for 3 months

[0104]

[0105]

[0106] in conclusion:

[0107] According to the data in the above table, compared with Comparative Example 3 in which chlorophyll is not added, Comparative Example 1 in which chlorophyll is replaced with anthocyanin, Comparative Example 4 in which beeswax is not added, and Comparative Example 2 in which beeswax is replaced with artificial fruit wax, the microcapsule preparation obtained in the embodiment has the best antioxidant effect and the conversion effect of NADH into NAD+ in cells.

[0108] The reason may be that the addition of chlorophyll affects the enzymatic reaction in the cell, speeding up the transfer of electrons from NADH to the electron transport chain, so that it is converted into NAD+ in the cell. Regarding beeswax, the presence of ether bonds in beeswax may increase the flexibility of beeswax molecules, allowing them to have a higher degree of bonding with other components; the chemical bonds formed by the dehydration reaction of the carboxyl group (-COOH) of the carboxylic acid and the hydroxyl group (-OH) of the alcohol in shellac wax are prone to hydrolysis, which destroys the molecular structure of shellac wax, thereby affecting the effects and the effects of interaction with other components, and thus affecting the quality of microcapsule preparations.

[0109] In addition, it was found during the experiment that the ultrasonic conditions in the preparation process also affect the quality of the microcapsule preparation. When the water bath ultrasonic conditions in step 1 are in the range of 25-28°C, the ultrasonic frequency is 30-40kHz, and the ultrasonic time is 12-25min; and the water bath heating and stirring conditions in step 3 are: temperature 30-40°C, stirring speed 70-110rmp / min, the surface of the prepared microcapsules is more shiny and uniform, without depressions, and has better quality.

[0110] Test Example 2:

[0111] The present application also studies the effect of taking the anti-aging capsule prepared in Example 4 on mood:

[0112] Subjects: 28 subjects (24 females and 4 males); subjects were told not to consume other foods containing NADH from 72 hours before the start of the study until the end of the study.

[0113] Average age: 78 years old

[0114] Capsules for consumption: NADH anti-aging capsules prepared in Example 4;

[0115] Dosage: Once a day, two capsules at a time, in the morning (500 mg / capsule);

[0116] Testing period: 3 months.

[0117] After the test, each person's score was measured and the improvement rate was calculated. The average improvement rate is shown in Figure 1 (Improvement in mood), Figure 2 (Improvements in memory and concentration). Figure 1 The evaluation criteria for emotions were based on the Hamilton Depression Rating Scale (HAMD assessment method); Figure 2 The evaluation criteria for the improvement of memory and attention are: SKT (Syndrom-Kurztest) short cognitive performance test.

[0118] Placebo: control group Verum: experimental group;

[0119] Before intake: before taking. After intake: after taking.

[0120] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A phospholipid-type NADH anti-aging capsule, characterized in that: The anti-aging capsule is prepared by mixing a NADH microcapsule preparation and an auxiliary agent and then adding the mixture into a capsule shell; The NADH microcapsule preparation is prepared by using NADH as the capsule core, chlorophyll as the middle layer, and a composite material of phospholipid and beeswax as the capsule wall to form a microcapsule, which is then dried.

2. The phospholipid type NADH anti-aging capsule according to claim 1, characterized in that: The microcapsule preparation comprises the following components by weight: NADH 5-15 parts, chlorophyll 5-15 parts, phospholipids 10-50 parts, beeswax 5-50 parts; The amount of the auxiliary agent added is 1.5-2.5 times the mass of the microcapsule preparation.

3. The phospholipid-type NADH anti-aging capsule according to claim 1, wherein the beeswax is obtained by compounding white beeswax and yellow beeswax; and the phospholipid is selected from soybean lecithin and sunflower lecithin.

4. The phospholipid type NADH anti-aging capsule according to claim 1, characterized in that: The beeswax is obtained by compounding white beeswax and yellow beeswax in a mass ratio of (1-3): (3-4); the phospholipid is obtained by compounding soybean lecithin and sunflower phospholipid in a mass ratio of (1-3): (4-6).

5. The phospholipid type NADH anti-aging capsule according to claim 1, characterized in that: The auxiliary agent is selected from biological ribose, coenzyme Q10, soybean lecithin, vitamins and minerals; the vitamins and minerals are selected from calcium, iron, zinc, selenium, copper, manganese, VC, VE, VB3, VB1, and VB2.

6. The phospholipid type NADH anti-aging capsule according to claim 5, characterized in that: The auxiliary agent is obtained by compounding biological ribose: coenzyme Q10: soybean lecithin: vitamins and minerals according to the mass ratio of (60-80): (45-50): (95-100): (30-33).

7. The phospholipid type NADH anti-aging capsule according to claim 1, characterized in that: The capsule shell is a hard capsule, including HPMC hydroxypropyl methylcellulose empty capsules and gelatin empty capsules.

8. The method for preparing the phospholipid type NADH anti-aging capsule according to any one of claims 1 to 7, characterized in that: include: Step 1: Take chlorophyll and place it in an ethanol solution to prepare a chlorophyll solution; pour it into a batching tank, introduce nitrogen to exhaust the air in the tank, add NADH and mix thoroughly to obtain a mixed solution, perform water bath ultrasound to completely dissolve NADH, filter, wash, and dry to obtain chlorophyll-encapsulated NADH lipid microcapsules; Step 2: heating beeswax, phospholipids and ethanol in a water bath and stirring until the beeswax and phospholipids are dissolved to obtain a beeswax-phospholipid complex; stirring and mixing the chlorophyll-coated NADH lipid microcapsules and the beeswax-phospholipid complex under vacuum stirring conditions; homogenizing and then spray drying to obtain a NADH microcapsule preparation; Step 3: Evenly mix the above NADH microcapsule preparation and the auxiliary agent to obtain a NADH microcapsule mixture, and fill the mixture into hard capsules to obtain a NADH capsule product.

9. The preparation method according to claim 8, characterized in that: In step 1, the conditions for water bath ultrasound are 25-28° C., ultrasound frequency: 30-40 kHz, and water bath ultrasound time: 12-25 min; The conditions for water bath heating and stirring in step 3 are: temperature 30-40° C., stirring speed 70-110 rpm.

10. Use of the phospholipid type NADH anti-aging capsule according to claim 1 in the preparation of anti-aging drugs.

Images