Pipetting device

Abstract

The invention relates to the technical field of life science instruments, and discloses a pipetting device. The pipetting device comprises a pipetting assembly, a bearing seat, a clamping assembly and a line supply assembly, the pipetting assembly comprises a pipetting body and a pipetting head arranged on the pipetting body, and the pipetting head is used for sucking or spitting liquid; the pipetting body is detachably connected with the bearing seat; the clamping assembly can take and place the pipetting body from the bearing seat and drive the pipetting body to move, so that the pipetting body moves to a preset position; the wire supply assembly comprises a cable, the length of the cable is adjustable, one end of the cable is arranged on the bearing seat, and the free end of the cable is connected with the liquid transfer assembly. Pipetting is achieved by moving the pipetting head, movement of the cell culture bottle is reduced, meanwhile, it can be guaranteed that the pipetting head is placed stably when not pipetting, and in addition, the problem that leads are wound mutually in the pipetting process is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of life science instruments, and in particular to a liquid transfer device. Background Art

[0002] When cells need to be passaged, the digested cell fluid in the cell culture bottle needs to be taken out and transferred to a centrifuge tube or other container. The commonly used technology at present is mainly to move the cell culture bottle under the liquid transfer device through a multi-degree-of-freedom mobile mechanism, then move the bottle mouth of the cell culture bottle upward or move the liquid adding gun head of the pipette into the bottle, and perform liquid aspiration. Another liquid transfer method is to pour the liquid in the bottle directly onto other containers. However, when the cell culture bottle is relatively large, such as using T175, multiple liquid transfers are required when performing cell passage. The specific operation process is: the mobile mechanism first moves the bottle to the lower part of the pipette, and then the pipette absorbs a certain amount of liquid, transfers the bottle away, and then transfers a new bottle, centrifuge tube or other container for holding liquid. Of course, the pipette can also be transferred to a new bottle for injection, and so on and so forth, until enough cell fluid is absorbed, the process of liquid transfer is terminated.

[0003] The above process requires multiple bottle placement and transfers. During the bottle transfer process, motion failures and bottle positioning problems are prone to occur. At the same time, the transfer process is long. If the pipette is moved to add liquid, the pipette leads often get tangled with each other and the fixed components wear out.

[0004] Therefore, a liquid transfer device is urgently needed to solve the above problems. Summary of the invention

[0005] Based on the above, the purpose of the present invention is to provide a pipetting device that achieves pipetting by moving the pipetting head, reduces the movement of the cell culture bottle, and at the same time ensures that the pipetting head is placed stably when not pipetting, and also reduces the problem of wire entanglement during the pipetting process.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] Liquid handling device, comprising:

[0008] A liquid transfer assembly, comprising a liquid transfer body and a liquid transfer head arranged on the liquid transfer body, wherein the liquid transfer head is used for aspirating or dispensing liquid;

[0009] A supporting seat, the pipetting body is detachably connected to the supporting seat;

[0010] A clamping assembly, capable of taking and placing the pipetting body from the supporting seat, and driving the pipetting body to move so as to move the pipetting body to a preset position;

[0011] The wire supply assembly comprises a cable, the length of the cable is adjustable, one end of the cable is arranged on the supporting seat, and the free end is connected to the pipetting assembly.

[0012] As a preferred solution of the pipetting device, one of the supporting seat and the pipetting body is provided with a male snap-fit ​​head, and the other is provided with a female snap-fit ​​head, and the male snap-fit ​​head can be snap-fitted with the female snap-fit ​​head.

[0013] As a preferred solution of the pipetting device, the supporting seat is provided with a limiting platform, and the pipetting body is provided with a limiting plate. When the male clamping head is clamped with the female clamping head, the limiting plate can be placed on the limiting platform.

[0014] As a preferred solution of the pipetting device, the male snap-in head is provided with a wedge-shaped protrusion, the female snap-in head is provided with a slot, and a bumper is provided in the slot. When the wedge-shaped protrusion is placed in the slot, the bumper can limit the male snap-in head in the female snap-in head.

[0015] As a preferred solution of the liquid transfer device, two of the male snap-fit ​​head and two of the female snap-fit ​​head are respectively provided and are arranged in one-to-one correspondence.

[0016] As a preferred solution of the pipetting device, the clamping assembly is selectively engaged with the pipetting assembly, and can drive the pipetting assembly to move back and forth between the supporting seat and the preset position when engaged with the pipetting assembly.

[0017] As a preferred solution of the pipetting device, the wire supply assembly includes a wire reel, one end of the cable is wound around the wire reel, the pipetting assembly is provided with an electric wire or a liquid tube, the electric wire or the liquid tube is fixedly connected to the cable in parallel, the other end of the cable is connected to the pipetting assembly, and the wire reel is used to reel in and out the cable and the electric wire or the liquid tube.

[0018] As a preferred solution of the pipetting device, the cable is configured as a spring wire, and the spring wire is an electric wire to power the pipetting head.

[0019] As a preferred solution of the pipetting device, the cable is configured as a spring wire, the spring wire is a liquid tube, and the liquid tube is connected to the pipetting head.

[0020] As a preferred solution of the liquid transfer device, a disposable nozzle is detachably provided on the liquid transfer head.

[0021] The beneficial effects of the present invention are:

[0022] The present invention is provided with a clamping assembly that can clamp and move the liquid transfer body, which is used to clamp and drive the liquid transfer assembly to a preset position for multiple suction or discharge, which is beneficial to improve the liquid transfer efficiency, and at the same time avoid the problems of liquid spilling and cell culture bottle falling caused by multiple transfers of cell culture bottles. By providing a bearing seat, the liquid transfer body can be detachably connected to the bearing seat, so that when no liquid transfer is required, the liquid transfer assembly can be placed on the bearing seat to ensure the stability of the liquid transfer head when it is not transferred. By providing a supply line assembly, and connecting one end of a length-adjustable cable to the liquid transfer assembly, when the liquid transfer assembly is separated from the bearing seat for liquid transfer, the cable can be adjusted in length according to the distance between the liquid transfer assembly and the bearing seat, effectively reducing the entanglement between various leads of the liquid transfer assembly and other wires; in general, the liquid transfer process of the liquid transfer device is simpler and more reliable. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0024] Figure 1 is a schematic diagram of a liquid transfer device provided in a specific embodiment of the present invention;

[0025] Figure 2 It is a perspective schematic diagram of a pipetting component of a pipetting device provided in a specific embodiment of the present invention;

[0026] Figure 3 It is a schematic diagram from another perspective of the pipetting component of the pipetting device provided in a specific embodiment of the present invention.

[0027] In the figure:

[0028] 100, pipetting assembly; 110, pipetting body; 111, female clamping head; 112, limiting plate; 120, pipetting head;

[0029] 200, bearing seat; 210, snap-on male connector;

[0030] 300, clamping assembly;

[0031] 400. Line supply assembly; 410. Line reel. DETAILED DESCRIPTION

[0032] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0033] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance. Among them, the terms "first position" and "second position" are two different positions.

[0034] Unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0036] The technical solution of the present invention is further described below with reference to the accompanying drawings and through specific implementation methods.

[0037] like Figure 1-Figure 3As shown, this embodiment provides a pipetting device, which includes a pipetting component 100, a supporting seat 200, a clamping component 300 and a supply line component 400. The pipetting component 100 includes a pipetting body 110 and a pipetting head 120 arranged on the pipetting body 110, and the pipetting head 120 is used for aspirating or spitting liquid; the pipetting body 110 is detachably connected to the supporting seat 200; the clamping component 300 can take and place the pipetting body 110 from the supporting seat 200, and drive the pipetting body 110 to move, so that the pipetting body 110 moves to a preset position; the supply line component 400 includes a cable, the cable length is adjustable, and one end of the cable is arranged on the supporting seat 200, and the free end is connected to the pipetting component 100.

[0038] By setting a clamping assembly 300 that can clamp and move the pipetting body 110, it is used to clamp and drive the pipetting assembly 100 to move to a preset position for multiple suction or discharge, which is conducive to improving the efficiency of pipetting, while avoiding the problems of liquid spilling and cell culture bottle falling caused by multiple transfers of cell culture bottles. By setting a supporting seat 200, the pipetting body 110 can be detachably connected with the supporting seat 200, so that when no pipetting is required, the pipetting assembly 100 can be placed on the supporting seat 200, ensuring the stability of the placement of the pipetting head 120 when not pipetting. By setting a supply line assembly 400, and connecting one end of a length-adjustable cable to the pipetting assembly 100, when the pipetting assembly 100 is separated from the supporting seat 200 for pipetting, the cable can be adjusted in length according to the distance between the pipetting assembly 100 and the supporting seat 200, effectively reducing the entanglement of various leads of the pipetting assembly 100 with other wires; in general, the pipetting process of the pipetting device is simpler and more reliable.

[0039] Specifically, the pipette head 120 can be a component for sucking and discharging liquid. In one embodiment, the pipette head 120 can be set as an independent pipette pump. In this case, the pipette pump needs wires for power supply and signal transmission; in other embodiments, the pipette head 120 can also be a stainless steel pipette tip. In this case, the stainless steel pipette tip needs to be connected to a liquid tube for sucking and discharging liquid. Preferably, the pipette head 120 is set as an ADP pipette.

[0040] Preferably, the pipetting head 120 is detachably provided with a disposable nozzle, which is replaced after one pipetting to ensure the sterility of each pipetting and avoid contamination caused by multiple pipetting.

[0041] Furthermore, the cable can also retract or stretch when driven by other elastic components. Exemplarily, the cable supply assembly 400 includes a cable reel 410, one end of the cable is wound around the cable reel 410, and the other end is fixedly connected to the pipetting assembly 100. The pipetting assembly 100 is provided with an electric wire or a liquid tube, and the electric wire or the liquid tube is fixedly connected to the cable, and the other end of the cable is connected to the pipetting assembly 100. The cable reel 410 can automatically reel in and unreel the cable and the electric wire or the liquid tube when the pipetting assembly 100 moves, thereby reducing the entanglement between the leads. Optionally, the cable is set to SUS304 stainless steel wire.

[0042] In other embodiments, the cable can also be a spiral wire or a spring wire that can be retracted under its own elastic force and can be stretched under the action of an external force. Exemplarily, the cable is set as a spring wire, and the spring wire is an electric wire to power the pipette head 120; or the spring wire is a liquid tube connected to the pipette head 120.

[0043] It is understandable that the longest retractable length of the cable is not less than the maximum distance between the pipetting assembly 100 and the supporting seat 200 .

[0044] As an optional solution for the pipetting device, one of the supporting seat 200 and the pipetting body 110 is provided with a snap-on male head 210, and the other is provided with a snap-on female head 111, and the snap-on male head 210 can be snap-on with the snap-on female head 111 to achieve the connection between the supporting seat 200 and the pipetting body 110. Preferably, there are two snap-on male heads 210 and two snap-on female heads 111, respectively, and they are arranged one by one, which is conducive to improving the reliability of the snap-on. Exemplarily, two groups of snap-on male heads 210 and snap-on female heads 111 are arranged at intervals along the vertical direction.

[0045] Specifically, the male clamping head 210 is provided with a wedge-shaped protrusion, and the female clamping head 111 is correspondingly provided with a slot, and a bumping ball is provided inside the slot. When the wedge-shaped protrusion is placed in the slot, the bumping ball can limit the male clamping head 210 in the female clamping head 111. It can be understood that the pre-tightening force of the bumping ball is not less than the gravity of the pipetting component 100 to ensure that the pipetting component 100 can be stably placed in the supporting seat 200. In other embodiments, the connection structure can also be set as a dovetail protrusion and dovetail groove that can be mutually engaged, or other elastic structures that can be engaged.

[0046] Preferably, the support seat 200 is provided with a limiting platform, and the pipette body 110 is provided with a limiting plate 112. When the male clamping head 210 is clamped with the female clamping head 111, the limiting plate 112 can be placed on the limiting platform. By providing the limiting platform and the limiting plate 112 that cooperate with each other, it is used to assist in carrying the pipette component 100, reduce the force between the male clamping head 210 and the female clamping head 111, and improve the reliability of the placement of the pipette component 100 on the support seat 200.

[0047] In this embodiment, the clamping assembly 300 is selectively engaged with the pipetting body 110 of the clamping pipetting assembly 100, and can drive it to and fro between the support seat 200 and the preset position when engaged therewith. It is understandable that the preset position can be a liquid aspiration position, a liquid spitting position, or a position where any pipetting head 120 needs to stay. The clamping connection is more reliable. In different embodiments, the selective connection between the clamping assembly 300 and the clamping pipetting assembly 100 can also be achieved through a magnetic connection. Optionally, the clamping assembly 300 can be moved by setting a mechanical arm with six degrees of freedom, and the action is more reliable.

[0048] It is worth noting that the pipetting device is also provided with a control system, and the driving parts are all connected to the control system in communication. The working process of the above-mentioned pipetting device is as follows: when working, when the pipetting device receives an instruction that a cell culture bottle needs to be subcultured, the cell culture bottle that has completed digestion has been placed in the transfer position and is tilted at a certain angle to adapt to the pipetting head 120; at the same time, the centrifuge tube that has been opened is placed in a relatively corresponding position. At this time, the moving arm drives the clamping assembly 300 to approach the pipetting assembly 100, and clamps the pipetting body 110, and moves horizontally or vertically to remove the pipetting assembly 100 from the supporting seat 200. Then replace the disposable suction nozzle of the pipetting head 120, and then the moving arm moves the pipetting assembly 100 to the position of the cell culture bottle and absorbs the liquid. After absorbing a certain amount of liquid, the moving arm moves the pipetting assembly 100 to the nearby centrifuge tube again and spits out the liquid. When there is a large amount of liquid in the cell culture bottle or there are multiple liquids of the same type of cell culture bottles that need to be absorbed, the absorption can be completed by repeating the above actions. After the suction action is completed, the moving arm moves the pipette component 100 to the solid waste area, removes the disposable nozzle, and finally the moving arm puts the pipette component 100 back to the supporting base 200. After the pipette component 100 is stably placed on the supporting base 200, the clamping component 300 can be disconnected from the pipette component 100.

[0049] The above contents are only preferred embodiments of the present invention. For ordinary technicians in this field, according to the concept of the present invention, there will be changes in the specific implementation methods and application scopes. The content of this specification should not be understood as limiting the present invention.

Claims

1. A liquid transfer device, characterized in that include: A liquid transfer assembly (100) comprises a liquid transfer body (110) and a liquid transfer head (120) arranged on the liquid transfer body (110), wherein the liquid transfer head (120) is used for aspirating or dispensing liquid; A supporting seat (200), the pipetting body (110) being detachably connected to the supporting seat (200); A clamping assembly (300) is capable of taking and placing the liquid transfer body (110) from the supporting seat (200) and driving the liquid transfer body (110) to move so as to move the liquid transfer body (110) to a preset position; A wire supply assembly (400) comprises a cable, the length of the cable is adjustable, one end of the cable is arranged on the supporting seat (200), and the free end is connected to the liquid transfer assembly (100).

2. The liquid transfer device according to claim 1, characterized in that: One of the supporting seat (200) and the pipetting body (110) is provided with a snap-fit ​​male head (210), and the other is provided with a snap-fit ​​female head (111), and the snap-fit ​​male head (210) can be snap-fitted with the snap-fit ​​female head (111).

3. The liquid transfer device according to claim 2, characterized in that: The supporting seat (200) is provided with a limiting platform, and the pipetting body (110) is provided with a limiting plate (112). When the male clamping head (210) is clamped with the female clamping head (111), the limiting plate (112) can be placed on the limiting platform.

4. The liquid transfer device according to claim 2, characterized in that: The male clamping head (210) is provided with a wedge-shaped protrusion, and the female clamping head (111) is provided with a clamping slot. A bumping ball is provided in the clamping slot. When the wedge-shaped protrusion is placed in the clamping slot, the bumping ball can limit the male clamping head (210) in the female clamping head (111).

5. The liquid transfer device according to claim 2, characterized in that: The male clamping head (210) and the female clamping head (111) are respectively provided with two, and are arranged in one-to-one correspondence.

6. The liquid transfer device according to claim 1, characterized in that: The clamping assembly (300) is selectively engaged with the liquid transfer assembly (100), and can drive the liquid transfer assembly (100) to move back and forth between the supporting seat (200) and the preset position when engaged with the liquid transfer assembly (100).

7. The liquid transfer device according to claim 1, characterized in that: The wire supply assembly (400) comprises a wire reel (410), one end of the cable is wound around the wire reel (410), the liquid transfer assembly (100) is provided with an electric wire or a liquid tube, the electric wire or the liquid tube is fixedly connected to the cable in parallel, the other end of the cable is connected to the liquid transfer assembly (100), and the wire reel (410) is used to reel in and unreel the cable and the electric wire or the liquid tube.

8. The liquid transfer device according to claim 1, characterized in that: The cable is configured as a spring wire, and the spring wire is an electrical wire to supply power to the pipetting head (120).

9. The liquid transfer device according to claim 1, characterized in that: The cable is configured as a spring wire, the spring wire is a liquid tube, and the liquid tube is connected to the pipetting head (120).

10. The liquid transfer device according to any one of claims 1 to 9, characterized in that: The pipetting head (120) is detachably provided with a disposable suction nozzle.

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