Immunopotentiator for improving anti-stress capability of aquatic animals and preparation method of immunopotentiator

Abstract

The invention discloses an immunopotentiator for improving the anti-stress capability of aquatic animals and a preparation method of the immunopotentiator. The immunopotentiator for improving the anti-stress capability of the aquatic animals is prepared from the following raw materials: spirulina powder; whey protein; a beta-glucan; glucosamine sulfate; potassium citrate; fish oil powder; algal polysaccharides; a nucleotide mixture; coating particles with vitamin C; a trace element chelate; according to the immunopotentiator capable of improving the anti-stress capacity of the aquatic animals and the preparation method of the immunopotentiator, the spirulina powder, the beta-glucan, the glucosamine sulfate and the nucleotide mixture are compounded, the anti-stress capacity of the aquatic animals is remarkably improved through the synergistic effect, and the survival rate of the aquatic animals is increased to 91% under the stress condition; the vitamin C coated particles, the fish oil powder and the algal polysaccharides are added, so that the antioxidant enzyme activity is effectively improved, the oxidation resistance of an immune system and the resistance to pathogenic bacteria are enhanced, and a remarkable immune enhancement effect is shown.

Description

Technical Field

[0001] The invention relates to an immunopotentiator for improving the anti-stress ability of aquatic animals and a preparation method thereof, belonging to the technical field of feed additives. Background Art

[0002] At present, the aquaculture industry is facing stress problems caused by environmental pollution, temperature fluctuations, transportation and other factors. These factors not only affect the health of aquatic animals, but may also lead to decreased immune function and increased disease incidence. In order to cope with these problems, various aquatic animal immune enhancers have appeared on the market. These enhancers usually enhance the anti-stress ability of aquatic animals by supplementing nutrients or adjusting the immune function in the body of aquatic animals.

[0003] Existing immune enhancers for aquatic animals have a single formula and lack complex ingredients, resulting in limited effects in improving stress resistance and immune function. They can easily burden the digestive system and metabolic process of aquatic animals, and long-term use may affect growth performance. In addition, the antioxidant capacity of the enhancers is insufficient and cannot effectively alleviate the oxidative stress problems of aquatic animals under environmental changes (such as temperature, transportation, hypoxia, etc.). Summary of the invention

[0004] The purpose of the present invention is to provide an immunopotentiator for improving the anti-stress ability of aquatic animals and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an immunopotentiator for improving the anti-stress ability of aquatic animals and a preparation method thereof, wherein the immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 8%-12%; whey protein: 10%-15%; β-glucan: 5%-10%; glucosamine sulfate: 3%-6%; potassium citrate: 2%-5%; fish oil powder: 8%-12%; seaweed polysaccharide: 6%-10%; nucleotide mixture: 3%-5%; vitamin C coated particles: 10%-15%; trace element chelates (zinc, selenium, copper): 2%-5%; cellulose carrier 18%-33%.

[0006] As a preferred technical solution of the present invention, the immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 10%; whey protein: 12%; β-glucan: 7%; glucosamine sulfate: 4%; potassium citrate: 3%; fish oil powder: 10%; seaweed polysaccharide: 8%; nucleotide mixture: 4%; vitamin C coated particles: 13%; trace element chelates (zinc, selenium, copper): 3%; cellulose carrier 26%.

[0007] As a preferred technical solution of the present invention, the immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 12%; whey protein: 15%; β-glucan: 9%; glucosamine sulfate: 5%; potassium citrate: 4%; fish oil powder: 12%; seaweed polysaccharide: 9%; nucleotide mixture: 3%; vitamin C coated particles: 10%; trace element chelates (zinc, selenium, copper): 3%; cellulose carrier 18%.

[0008] As a preferred technical solution of the present invention, the immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 8%; whey protein: 10%; β-glucan: 5%; glucosamine sulfate: 3%; potassium citrate: 2%; fish oil powder: 8%; seaweed polysaccharide: 6%; nucleotide mixture: 5%; vitamin C coated particles: 15%; trace element chelates (zinc, selenium, copper): 5%; cellulose carrier 33%.

[0009] As a preferred technical solution of the present invention, the particle size of the spirulina powder is 50 microns to 80 microns.

[0010] As a preferred technical solution of the present invention, the source of the β-glucan is yeast cell wall with a purity of ≥85%.

[0011] As a preferred technical solution of the present invention, the molar ratio of zinc, selenium and copper in the trace element chelate is 2:1:1.

[0012] As a preferred technical solution of the present invention, the preparation method of the immunopotentiator for improving the anti-stress ability of aquatic animals is as follows: Step 1: Spirulina powder treatment: After passing the Spirulina powder through a 100-mesh sieve, dry it at 60°C for 2 hours and set aside; Step 2: Mixing step: weigh whey protein, β-glucan, glucosamine sulfate, potassium citrate, fish oil powder, seaweed polysaccharide, nucleotide mixture, vitamin C coated particles and trace element chelate according to proportion; use a horizontal mixer to stir at 300 rpm for 15 minutes to prepare a uniform mixture; Step 3: Wet granulation: add distilled water to the mixture and adjust the moisture content to 20%; use a wet granulator to prepare granules with a particle size of 0.5-1.0 mm; Step 4: Drying step: Place the wet granules in a dryer and dry at 50°C for 6h-7h, controlling the moisture content of the finished product to ≤10%; Step 5: Packaging: Cool the dried particles to room temperature, place them in a moisture-proof vacuum bag and seal them to obtain the enhancer.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention significantly improves the anti-stress ability of aquatic animals through the synergistic effect of compounding spirulina powder, β-glucan, glucosamine sulfate and a nucleotide mixture. Under stress conditions, the survival rate of aquatic animals is increased to 91%. In addition, vitamin C-coated particles, fish oil powder and seaweed polysaccharides are added to effectively improve the activity of antioxidant enzymes, enhance the antioxidant capacity of the immune system and the resistance to pathogens, and show a significant immune enhancement effect. In addition, the use of bio-friendly ingredients such as whey protein and potassium citrate effectively avoids irritation, reduces the burden on the digestive system of aquatic animals, and promotes protein metabolism and energy conversion. DETAILED DESCRIPTION

[0014] The technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] The present invention provides an immunopotentiator for improving the anti-stress ability of aquatic animals and a preparation method thereof. Embodiment 1: The immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 10%; whey protein: 12%; β-glucan: 7%; glucosamine sulfate: 4%; potassium citrate: 3%; fish oil powder: 10%; seaweed polysaccharide: 8%; nucleotide mixture: 4%; vitamin C coated particles: 13%; trace element chelate (zinc, selenium, copper): 3%; cellulose carrier 26%.

[0016] Example 2: The immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 12%; whey protein: 15%; β-glucan: 9%; glucosamine sulfate: 5%; potassium citrate: 4%; fish oil powder: 12%; seaweed polysaccharide: 9%; nucleotide mixture: 3%; vitamin C coated particles: 10%; trace element chelates (zinc, selenium, copper): 3%; cellulose carrier 18%.

[0017] Example 3: The immunopotentiator for improving the anti-stress ability of aquatic animals is formulated according to the following raw materials: spirulina powder: 8%; whey protein: 10%; β-glucan: 5%; glucosamine sulfate: 3%; potassium citrate: 2%; fish oil powder: 8%; seaweed polysaccharide: 6%; nucleotide mixture: 5%; vitamin C coated particles: 15%; trace element chelates (zinc, selenium, copper): 5%; cellulose carrier 33%.

[0018] Wherein, the particle size of the spirulina powder is 50 microns to 80 microns.

[0019] Preferably, the source of the β-glucan is yeast cell wall, and the purity is ≥85%.

[0020] Furthermore, the molar ratio of zinc, selenium and copper in the trace element chelate is 2:1:1.

[0021] The preparation method of the immunopotentiator for improving the anti-stress ability of aquatic animals is as follows: Step 1: Spirulina powder treatment: After passing the Spirulina powder through a 100-mesh sieve, dry it at 60°C for 2 hours and set aside; Step 2: Mixing step: weigh whey protein, β-glucan, glucosamine sulfate, potassium citrate, fish oil powder, seaweed polysaccharide, nucleotide mixture, vitamin C coated particles and trace element chelate according to proportion; use a horizontal mixer to stir at 300 rpm for 15 minutes to prepare a uniform mixture; Step 3: Wet granulation: add distilled water to the mixture to adjust the moisture content to 20%; use a wet granulator to prepare granules with a particle size of 0.5-1.0 mm; Step 4: Drying step: Place the wet granules in a dryer and dry at 50°C for 6h-7h, controlling the moisture content of the finished product to ≤10%; Step 5: Packaging: Cool the dried particles to room temperature, place them in a moisture-proof vacuum bag and seal them to obtain the enhancer.

[0022] Experimental purpose: To verify whether the aquatic animal anti-stress immune enhancer of the present invention is superior to the immune enhancers in the prior art in improving the anti-stress ability and survival rate: Experimental design: 1. Experimental subjects Experimental animals: Healthy tilapia (average weight 50±2g) were selected and randomly divided into 3 groups, with 60 fish in each group.

[0023] Experimental groups: Control group: basic feed without any immune enhancer.

[0024] Prior art group: feed with commercially available β-glucan + fish oil powder (ratio of 5% + 10%) enhancers added.

[0025] The present invention group: feed to which the immunopotentiator of the present invention is added.

[0026] Experimental conditions Breeding environment: temperature 26-28℃, dissolved oxygen ≥5mg / L, pH 7.5±0.2, and breeding density 10 per cubic meter.

[0027] Feeding method: Feed twice a day (08:00 and 16:00), the feeding amount is 3% of the total weight of the fish, and the experimental period is 60 days.

[0028] Test indicators Stress resistance: The survival rate of fish is tested through a simulated transportation stress experiment (24-hour low oxygen environment).

[0029] Growth performance: Determine the weight gain rate and feed conversion rate after the experiment; 1. Stress resistance (survival rate) 2. Growth performance Comparative Analysis 1. Anti-stress ability The survival rate of the group of the present invention (91%) was significantly higher than that of the prior art group (81%) and the control group (68%), indicating that the enhancer of the present invention can more effectively alleviate the loss of aquatic animals in transportation stress. The synergistic effect of β-glucan, nucleotide mixture and glucosamine sulfate in the formula can significantly enhance the anti-stress ability. 2. Growth performance The group of the present invention performed best in weight gain rate and feed conversion rate. Its feed conversion rate was 1.1, much lower than 1.3 of the prior art group, indicating that feed utilization efficiency was higher. The scientific ratio of whey protein, fish oil powder and seaweed polysaccharide helped to enhance the growth and metabolic function of fish.

[0030] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An immunopotentiator for improving the anti-stress ability of aquatic animals and a preparation method thereof, characterized in that: The immunopotentiator for improving the anti-stress ability of aquatic animals is prepared according to the following raw materials: spirulina powder: 8%-12%; whey protein: 10%-15%; beta-glucan: 5%-10%; glucosamine sulfate: 3%-6%; potassium citrate: 2%-5%; fish oil powder: 8%-12%; seaweed polysaccharide: 6%-10%; nucleotide mixture: 3%-5%; vitamin C coated particles: 10%-15%; trace element chelates (zinc, selenium, copper): 2%-5%; and cellulose carrier 18%-33%.

2. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The immunopotentiator for improving the anti-stress ability of aquatic animals is prepared according to the following raw materials: spirulina powder: 10%; whey protein: 12%; beta-glucan: 7%; glucosamine sulfate: 4%; potassium citrate: 3%; fish oil powder: 10%; seaweed polysaccharide: 8%; nucleotide mixture: 4%; vitamin C coated particles: 13%; trace element chelate (zinc, selenium, copper): 3%; and cellulose carrier 26%.

3. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The immunopotentiator for improving the anti-stress ability of aquatic animals is prepared according to the following raw materials: spirulina powder: 12%; whey protein: 15%; beta-glucan: 9%; glucosamine sulfate: 5%; potassium citrate: 4%; fish oil powder: 12%; seaweed polysaccharide: 9%; nucleotide mixture: 3%; vitamin C coated particles: 10%; trace element chelate (zinc, selenium, copper): 3%; and cellulose carrier 18%.

4. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The immunopotentiator for improving the anti-stress ability of aquatic animals is prepared according to the following raw materials: spirulina powder: 8%; whey protein: 10%; beta-glucan: 5%; glucosamine sulfate: 3%; potassium citrate: 2%; fish oil powder: 8%; seaweed polysaccharide: 6%; nucleotide mixture: 5%; vitamin C coated particles: 15%; trace element chelate (zinc, selenium, copper): 5%; and cellulose carrier 33%.

5. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The particle size of the spirulina powder is 50 microns to 80 microns.

6. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The beta-glucan is derived from yeast cell walls, and has a purity of ≥85%.

7. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The molar ratio of zinc, selenium and copper in the trace element chelate is 2:1:

1.

8. The immunopotentiator for improving the anti-stress ability of aquatic animals and its preparation method according to claim 1, characterized in that: The preparation method of the immunopotentiator for improving the anti-stress ability of aquatic animals is as follows: Step 1: Spirulina powder treatment: After passing the Spirulina powder through a 100-mesh sieve, dry it at 60°C for 2 hours and set aside; Step 2: Mixing step: weigh whey protein, β-glucan, glucosamine sulfate, potassium citrate, fish oil powder, seaweed polysaccharide, nucleotide mixture, vitamin C coated particles and trace element chelate according to proportion; use a horizontal mixer to stir at 300 rpm for 15 minutes to prepare a uniform mixture; Step 3: Wet granulation: add distilled water to the mixture and adjust the moisture content to 20%; use a wet granulator to prepare granules with a particle size of 0.5-1.0 mm; Step 4: Drying step: Place the wet granules in a dryer and dry at 50°C for 6h-7h, controlling the moisture content of the finished product to ≤10%; Step 5: Packaging: Cool the dried particles to room temperature, place them in a moisture-proof vacuum bag and seal them to obtain the enhancer.

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