Application of trans-anisd in relieving toxicity of zearalenone in animals

Abstract

The invention discloses application of trans-anisd in relieving toxicity of zearalenone in animals, and belongs to the technical field of prevention and treatment of mycotoxin. The invention provides an application of trans-anisd in preparation of a medicine or feed for relieving zearalenone poisoning of animals. The trans-anisd is proved to be capable of effectively relieving piglet reproductive toxicity caused by zearalenone, improving uterine organ indexes and ovarian organ indexes and ensuring piglet health and reproductive system development, and a new method is provided for zearalenone detoxification.

Description

Technical Field

[0001] The invention relates to the technical field of mycotoxin prevention and control, and in particular to application of trans-anisole in alleviating the toxicity of zearalenone in animals. Background Art

[0002] Zearalenone poisoning in pigs is a type of poisoning caused by pigs eating grain feed contaminated by zearalenone. Sick pigs will develop reproductive system diseases, and the disease is common in piglets aged 3 to 5 months. Zearalenone (ZEN) is a toxic metabolite produced by Fusarium graminearum, Fusarium oxysporum, Fusarium equisetum, Fusarium nivalis and other species. It is mainly found in corn, wheat, sorghum, rice and other grains contaminated by fungi. Studies have shown that zearalenone has an estrogen-like effect on sows with ovariotomy, which can cause pigs to show hyperestrogenism and fungal inflammatory reactions in the vaginal mucosa. In the last month of pregnancy, pregnant sows injected with 5 mg of zearalenone every day can cause the birth of dead piglets. Zearalenone has caused great harm to pig breeding and the pig farming industry.

[0003] In principle, the removal methods of zearalenone can be divided into two types: detoxification and detoxification. According to the methods, they can be roughly divided into physical methods, chemical methods, and biological methods. Detoxification refers to the method of removing or neutralizing mycotoxins in contaminated feed, and detoxification refers to the method of removing the toxicity of mycotoxins. Physical removal methods include mechanical classification treatment, high-temperature inactivation, radiation treatment or extraction of contaminated and adsorbents, etc., but zearalenone is not easily soluble in water and is resistant to high temperatures, and repeated treatments are required to remove some toxins. The chemical removal method is to treat mycotoxins through acid-base solutions or other compounds, but excessive chemical reagents, such as ammonia residues in feed, may endanger animal health. Biological detoxification methods mainly use enzymes produced by microorganisms, plants and their metabolism to degrade toxins. For example, Clonostachys rosea IFO7063 can secrete specialized lactonase to effectively transform zearalenone; detoxifying Trichosporon (T.mycotoxinivorans, MTV) has a unique "swallowing" property and can remove the toxicity of zearalenone; Pseudomonas putida can completely degrade zearalenone; Bacillus subtilis (B.subtilis), Bacillus licheni-formis (B.licheni-formis) and some Rhizopus (Rhizopusstolonifer, Rhizopusoryzae, Rhizopusmicrosporus) can also degrade zearalenone. This type of detoxification method has mild conditions and less damage to feed nutrients. The disadvantage is that some toxins require some complete mold systems to be completely detoxified, and most molds are unstable. Therefore, how to effectively remove zearalenone, alleviate zearalenone poisoning in pigs, and improve the economic benefits of the pig farming industry has become an urgent problem to be solved in this field. Summary of the invention

[0004] The purpose of the present invention is to provide an application of trans-anisole in alleviating the toxicity of zearalenone in animals to solve the problems existing in the above-mentioned prior art. The present invention confirms that trans-anisole can effectively alleviate the reproductive toxicity of piglets caused by zearalenone, improve the uterine organ index and ovarian organ index, ensure the health of piglets and the development of the reproductive system, and provide a new method for the detoxification of zearalenone.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The invention provides application of trans-anisole in preparing a medicine for alleviating zearalenone poisoning in animals.

[0007] Preferably, the animal comprises a piglet.

[0008] The invention also provides application of trans-anisole in preparing feed for alleviating zearalenone poisoning in animals.

[0009] Preferably, the animal comprises a piglet.

[0010] The present invention also provides a medicine for alleviating zearalenone poisoning in animals, wherein the main component of the medicine is trans-anisole.

[0011] Preferably, the medicine further contains pharmaceutically acceptable excipients.

[0012] Preferably, the animal comprises a piglet.

[0013] The invention also provides a feed for alleviating zearalenone poisoning in animals, wherein the feed contains trans-anisole.

[0014] Preferably, the added concentration of trans-anisole in the feed is 500 mg / kg.

[0015] Preferably, the animal comprises a piglet.

[0016] The present invention discloses the following technical effects:

[0017] Trans-anisole, CAS No.: 4180-23-8, alias: trans-4-propenylanisole, trans-anethole, extracted and separated from the plant star anise, has high biological safety. The present invention proves that adding trans-anisole to piglet feed contaminated with zearalenone can effectively alleviate the reproductive toxicity caused by zearalenone, improve the uterine organ index and ovarian organ index, and ensure the health of animals and the development of the reproductive system. The present invention provides a new resource for the biological detoxification of zearalenone, and lays a theoretical foundation for the development of drugs and feeds to alleviate animal zearalenone poisoning. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 The effect of zearalenone and trans-anisole on liver organ index of weaned piglets (g / kg, n=6); A is the liver phenotype of weaned piglets, and B is the statistical chart of liver organ index of weaned piglets;

[0020] Figure 2 The effect of zearalenone and trans-anisole on the uterine organ index of weaned piglets (g / kg, n=6); A is the uterine phenotype of weaned piglets, and B is the statistical chart of the uterine organ index of weaned piglets;

[0021] Figure 3 The figure shows the effects of zearalenone and trans-anisole on the ovarian organ index of weaned piglets (g / kg, n=6); wherein A is the ovarian phenotype of weaned piglets, and B is the statistical chart of the ovarian organ index of weaned piglets. DETAILED DESCRIPTION

[0022] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0023] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0024] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0025] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0026] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0027] The biological materials, reagents and feeds in the embodiments of the present invention can all be purchased through conventional channels.

[0028] Example 1

[0029] 1. Materials and reagents

[0030] Zearalenone (ZEA): chromatographically pure: purity ≥ 98%, purchased from Fermentek (Jerusalem, Israel).

[0031] Trans-anisole (TA): CAS number: 4180-23-8, purity ≥99%, slightly yellow liquid at room temperature, purchased from Guangdong Wengjiang Chemical Reagent Co., Ltd.

[0032] 2. Experimental methods

[0033] 2.1 Experimental Grouping

[0034] Twenty-four healthy 28-day-old three-way crossbred (Durham × Longhorn × Longhorn) weaned gilts were selected and transported to the experimental site at 35 days of age. They were fed with farm feed for the first 3 days and then gradually transitioned to the experimental basal feed. At 42 days of age, the piglets were randomly divided into 4 treatments, with 6 replicates in each treatment (one gilt in each replicate). After the grouping was completed, the pre-test began. Treatment 1 was fed with a basal diet, which was the control group (CON); Treatment 2 added 1.5 mg / kg of zearalenone to the basal diet, which was the zearalenone group (ZEA); Treatment 3 added 500 mg / kg of trans-anisole to the basal diet, which was the trans-anisole group (TA); Treatment 4 added 1.5 mg / kg of zearalenone and 500 mg / kg of trans-anisole to the basal feed, which was the trans-anisole group to alleviate the toxicity of zearalenone (ZEA×TA). The pre-test period was 3 days, and the formal test period was 28 days. The basic feed was formulated according to the standard formula for pigs of NRC (2012). The formula and main nutrients are shown in Table 1.

[0035] Table 1 Basic diet composition and nutrient content (air-dried basis) %

[0036]

[0037] Note: The premix provides the following per kilogram of feed: VA2330 IU; VD3250 IU; VC 150IU; VE 50IU; VK30.65mg; VB12.00mg; VB24.70 mg; pantothenic acid 14.00mg; niacin 22.00mg; pyridoxine 2.20mg; biotin 0.10mg; folic acid 0.50mg; VB120.02 mg; manganese (methionine manganese) 4.50mg; iron (ferrous fumarate) 99mg; zinc (zinc glycinate) 99mg; copper (copper glycinate) 6.50mg; iodine (calcium iodate) 0.15mg; selenium (yeast selenium) 0.33mg.

[0038] 2.2 Feeding and management

[0039] Gilts are raised in single metabolic cages, which are equipped with fixed feed troughs, duckbill drinkers and slatted floors. The feed troughs and drinkers are placed at a certain distance to ensure that the gilts can eat and drink freely while ensuring that the feed is dry. Before the piglets enter the experimental station, a comprehensive inspection of the pig house facilities and equipment is carried out; the inside and outside of the pig house are thoroughly cleaned and disinfected. After the gilts enter the experimental station, the outside of the house is disinfected every day and the inside of the house is disinfected every week to ensure daily air circulation in the pig house, and the excrement of the pig house is cleaned every morning and evening. The temperature in the house is about 30°C in the first week of the experiment. From the second week, the temperature is maintained at 25-28°C, and the relative humidity is maintained at about 65%. Other feeding and management procedures are carried out according to routine procedures until the end of the experiment.

[0040] 2.3 Experimental diet preparation

[0041] ZEA test diet: After fully dissolving ZEA with a certain amount of ethyl acetate, spray it evenly on a certain amount of talcum powder and mix well to obtain a ZEA premix (1000 mg / kg); place it in a fume hood at room temperature for 24 hours; after the ethyl acetate is completely volatilized, mix the premix with an appropriate amount of basal diet step by step to obtain a 1.5 mg / kg ZEA test diet.

[0042] TA test diet: Take an appropriate amount of trans-anisole and mix it evenly with the basal diet step by step to obtain 500 mg / kg TA test diet.

[0043] ZEA×TA test diet: trans-anisole was measured and mixed evenly with the ZEA-treated diet step by step to obtain 1.5 mg / kg ZEA×500 mg / kg TA test diet.

[0044] All test diets were sealed and stored in a cool, dry and ventilated place. According to the "Feed Sampling Method" (GB / T14699.1-2005), the test diets were sampled before and after the experiment to test their conventional nutrients and toxin levels.

[0045] 2.4 Determination of conventional nutrients and toxin levels in feed

[0046] The determination of conventional nutrients such as dry matter (DM, 100±3℃ drying method), crude ash (CA, 550℃ ashing method), organic matter (OM, OM=DM-CA), crude protein (CP, Kjeldahl method) and crude fat (EE, Soxhlet extraction method) in the feed was carried out according to AOAC (2012), and amino acids were determined using an automatic amino acid analyzer.

[0047] The quantitative analysis of ZEA, fumonisin (FUM), vomitoxin (DON) and aflatoxin B1 (AFB1) in feed was carried out by immunoaffinity column purification-high performance liquid chromatography, and their minimum detection limits were 0.01 mg / kg, 0.25 mg / kg, 0.1 mg / kg and 1.0 μg / kg, respectively. The test results showed that the ZEA content of the experimental diet in the control group, TA group, ZEA group and ZEA×TA group was 0, 1.50±mg / kg and 1.50±mg / kg, respectively. FUM, DON and AFB1 were not detected or were below the detection limit.

[0048] 2.5 Growth performance determination

[0049] Weigh and record each gilt before and after the formal experiment, and accurately record the gilt's feed intake and residual feed every day. Calculate the average daily feed intake (ADFI), average daily weight gain (ADG) and feed-to-weight ratio (F / G) of piglets using the following formula:

[0050] ADFI (kg / d) = feed amount - residual material amount

[0051] ADG (kg / d) = (final weight - initial weight) / number of test days

[0052] F / G=ADFI / ADG

[0053] 2.6 Nutrient apparent digestibility determination

[0054] The apparent digestibility of nutrients was determined by the full feces collection method on the 26th to 28th day of the formal trial period. All fresh feces of gilts were collected every day, 1 / 10 of the feces sample was taken after evenly mixing, and two tubes of 2 mL of fresh feces were stored in -80℃ liquid nitrogen for microbial testing, and the rest were stored at 4℃. After the feces sample collection was completed, the feces samples of each gilt for 3 days were mixed, and 100g of feces samples were added with 10mL of 10% sulfuric acid to fix nitrogen for crude protein (CP, Kjeldahl method). The remaining samples were dried to constant weight in a constant temperature drying oven at 60±3℃, and the samples were crushed for determination of dry matter (DM, 100±3℃ drying method), crude ash (CA, 550℃ ashing method), organic matter (OM, OM=DM-CA) and crude fat (EE, Soxhlet extraction method). The conventional component determination was referred to AOAC (2012).

[0055] 2.7 Blood sample collection and determination

[0056] Blood was collected from the anterior vena cava before feeding on the 28th day of the formal test period. 5 mL of blood was collected using an anticoagulant tube, and the blood was sent for testing if there was no coagulation phenomenon after standing for 15 minutes, and the whole blood index was measured; 5 mL of blood was collected using a procoagulant tube, and the blood was centrifuged at 3,500 r / min for 15 minutes at 4°C after standing at room temperature for 15 minutes, and the serum was separated and stored at -20°C for testing of serum enzymes, metabolites, hormones and antioxidant indicators.

[0057] 2.7.1 Hematological indicators

[0058] Hematological indices were measured using a blood cell analyzer (KX-21, SYSEMX, Japan), including total white blood cell count (WBC), total red blood cell count (RBC), total platelet count (PLT), lymphocytes (LYM), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), hemoglobin content (MCH), and hemoglobin concentration (MCHC).

[0059] 2.7.2 Serum metabolites

[0060] Serum biochemical indicators were analyzed using a fully automatic serum biochemical analyzer (COBUS MIRAPlus, Roche, USA), including total protein (TP), albumin (ALB), urea nitrogen (UREA), glucose (GLU), triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL).

[0061] 2.7.3 Serum enzymes

[0062] Serum enzymes were analyzed using an automatic serum biochemical analyzer (COBUS MIRAPlus, Roche, USA), including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH).

[0063] 2.7.4 Serum hormones

[0064] Serum hormones were measured using a radioimmunoassay instrument, including follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and progesterone (P).

[0065] 2.7.5 Serum antioxidant enzymes

[0066] The determination of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) was carried out according to the operating procedures of the kit. For the determination of SOD, the sample was added to the centrifuge tube according to the steps of the kit, mixed evenly, placed in a 37°C water bath for heating for 40 minutes, and the color developer was added to determine the absorbance value; for the determination of GSH-PX, the sample was added to the centrifuge tube according to the steps of the kit, 1 mL of the supernatant was taken for color reaction after centrifugation, and the color developer was added and allowed to stand at room temperature for 15 minutes, and the absorbance value was determined; for the determination of MDA, the sample was added according to the steps of the kit, mixed evenly with a vortex mixer, placed in a 95°C water bath for 40 minutes, taken out and cooled with running water, and the supernatant was taken after centrifugation for absorbance determination. The absorbance (OD) value was determined using an ultraviolet spectrophotometer. A 1-cm light path cuvette was used for colorimetry. The cuvettes were paired before use. The instrument was turned on and preheated for 20 minutes. The wavelengths (550 nm, 412 nm, and 532 nm) were adjusted, and the index values ​​of the samples were calculated according to the formula.

[0067] 2.8 Collection and determination of slaughter samples

[0068] After the formal test period, the gilts in each group were stunned and bled before being slaughtered. The abdominal cavity was opened, and the liver, uterus, ovaries and other organs were first observed and recorded for obvious pathological changes. The spleen, heart, kidneys, liver, uterus and ovaries were quickly taken, weighed and recorded to calculate the organ index. The calculation formula is as follows:

[0069] Organ index (g / kg) = organ weight (g) / live body weight (kg)

[0070] The uterus was rinsed with saline and then cut and sampled in a 5 mL cryotube at -80°C for transcriptomics detection. Another portion was fixed in 4% paraformaldehyde solution for hematoxylin-eosin (HE) staining and immunohistochemistry. One ovary was stored in a 5 mL cryotube at -80°C, and the other in fixative. When sampling, ensure that each repeated uterus sampling site is the same and the ovary is on the same side.

[0071] 3. Experimental results

[0072] 3.1 Growth performance

[0073] The effects of zearalenone and trans-anisole on the growth performance of weaned piglets are shown in Table 2. The results showed that 1.5 mg / kg zearalenone and 500 mg / kg trans-anisole had no significant effect on the growth performance of weaned piglets (P>0.05). Zearalenone and trans-anisole had a significant interactive effect on the average daily weight gain of weaned piglets (P<0.05).

[0074] Table 2 Effects of zearalenone and trans-anisole on the production performance of weaned piglets (n=6)

[0075]

[0076] Note: CON: control; ZEA: zearalenone; TA: trans-anisole.

[0077] 3.2 Nutrient Apparent Digestibility

[0078] The effects of zearalenone and trans-anisole on the apparent digestibility of nutrients in weaned piglets are shown in Table 3. The results showed that 1.5 mg / kg zearalenone significantly reduced the digestibility of protein in weaned piglets (P<0.05), and 500 mg / kg trans-anisole had no significant effect on the apparent digestibility of nutrients in weaned piglets (P>0.05). There was no significant interaction between zearalenone and trans-anisole on the apparent digestibility of nutrients in weaned piglets (P>0.05).

[0079] Table 3 Effects of zearalenone and trans-anisole on apparent nutrient digestibility in weaned piglets (%, n = 6)

[0080]

[0081] Note: CON: control; ZEA: zearalenone; TA: trans-anisole.

[0082] 3.3 Hematological indicators

[0083] The effects of zearalenone and trans-anisole on the hematological parameters of weaned piglets are shown in Table 4. The results showed that 1.5 mg / kg zearalenone and 500 mg / kg trans-anisole had no significant effect on the measured hematological parameters (P>0.05). There was no significant interaction between zearalenone and trans-anisole on the hematological parameters of piglets (P>0.05).

[0084] Table 4 Effects of zearalenone and trans-anisole on hematological parameters of weaned piglets (n=6)

[0085]

[0086]

[0087] Note: CON: control; ZEA: zearalenone; TA: trans-anisole.

[0088] 3.4 Serum metabolites

[0089] The effects of zearalenone and trans-anisole on serum biochemical parameters of weaned piglets are shown in Table 5. The results showed that 1.5 mg / / kg zearalenone significantly reduced serum total protein (TP), albumin (ALB) and high-density lipoprotein (HDL) (P<0.05), urea nitrogen (UREA) and low-density lipoprotein (LDL) (P<0.05), and total cholesterol (TC) tended to increase (P=0.05). 500 mg / kg trans-anisole significantly reduced serum TC (P<0.05), and significantly increased HDL (P<0.05). Zearalenone and trans-anisole had an interactive trend on TC (P=0.060).

[0090] Table 5 Effects of zearalenone and trans-anisole on serum metabolites in weaned piglets (U / mL, n=6)

[0091]

[0092] Note: CON control; ZEA: zearalenone; TA: trans-anisole.

[0093] 3.5 Serum enzymes

[0094] The effects of zearalenone and trans-anisole on serum enzymes of weaned piglets are shown in Table 6. 1.5 mg / kg zearalenone significantly increased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in the serum of piglets (P<0.05). 500 mg / kg trans-anisole significantly reduced the levels of ALT, AST and LDH (P<0.05). Zearalenone and trans-anisole had a significant interaction on ALT and alkaline phosphatase (ALP) (P<0.05).

[0095] Table 6 Effects of zearalenone and trans-anisole on serum enzyme activities in weaned piglets (U / ml, n=6)

[0096]

[0097]

[0098] Note: CON: control; ZEA: zearalenone; TA: trans-anisole.

[0099] 3.6 Serum hormones

[0100] The effects of zearalenone and trans-anisole on serum hormones in weaned piglets are shown in Table 7. 1.5 mg / kg zearalenone significantly reduced the levels of follicle-stimulating hormone (FSH) and estradiol (E2) (P<0.05), and there was a trend of decreasing luteinizing hormone (LH) (P=0.060) and progesterone (PG) (P=0.079). 500 mg / kg trans-anisole had no significant effect on the serum hormone levels of weaned piglets (P>0.05). There was no significant interaction between zearalenone and trans-anisole on the serum hormone levels of weaned piglets (P>0.05).

[0101] Table 7 Effects of zearalenone and trans-anisole on serum enzyme activities in weaned piglets (n=6)

[0102]

[0103] 3.7 Serum antioxidant markers

[0104] The effects of zearalenone and trans-anisole on serum antioxidant markers in weaned piglets are shown in Table 8. The results showed that 1.5 mg / kg zearalenone significantly increased the content of malondialdehyde (MDA) (P<0.05), and significantly reduced the content of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) (P<0.05). 500 mg / kg trans-anisole had a tendency to reduce MDA in weaned piglets (P=0.099) and promote the production of SOD (P=0.087). Zearalenone and trans-anisole had a significant interaction on the peroxidation marker MDA in weaned piglets (P<0.05), and had an interactive trend on GSH-Px (P=0.085) and SOD (P=0.074).

[0105] Table 8 Effects of zearalenone and trans-anisole on serum enzyme activities in weaned piglets (n=6)

[0106]

[0107] 3.8 Organ Index

[0108] 3.8.1 Liver organ index

[0109] Effects of zearalenone and trans-anisole on liver indexes in weaned piglets Figure 1 As shown. 1.5 mg / kg of zearalenone and 500 mg / kg of trans-anisole had no significant effect on the liver index of piglets (P<0.05). There was no significant interaction between zearalenone and trans-anisole on the liver index of piglets (P>0.05).

[0110] 3.8.2 Uterine organ index

[0111] Effects of zearalenone and trans-anisole on uterine organ indexes in weaned piglets Figure 2 As shown. 1.5 mg / kg zearalenone significantly increased the uterine organ index of piglets (P<0.05). Compared with the CON group, the uterus of the ZEA group showed obvious edema and inflammatory response; 500 mg / kg trans-anisole had no significant effect on the uterine organ index of piglets (P>0.05). The uterine volume of the TA group was larger than that of the CON group, but there was no obvious edema and inflammatory response, which was normal development, indicating that trans-anisole has a lower estrogen effect, can competitively inhibit the toxicity of ZEA, and has the effect of promoting uterine development. Zearalenone and trans-anisole had a significant interaction on the uterine organ index of piglets (P<0.05). Compared with the ZEA group, the degree of uterine edema and inflammatory response in the ZEA×TA group were reduced. It shows that trans-anisole alleviates the toxic effect of zearalenone on the uterus.

[0112] 3.8.3 Ovarian organ index

[0113] Effects of zearalenone and trans-anisole on ovarian organ indexes in weaned piglets Figure 3 As shown. 1.5mg / kg zearalenone significantly reduced the ovarian organ index of weaned piglets (P<0.05). Compared with the CON group, the ovarian volume of the ZEA group was significantly reduced, the number of surface follicles was significantly reduced, and the ovaries were incompletely developed; 500mg / kg trans-anisole had a trend of increasing the ovarian organ index of piglets (P=0.051). The ovarian surface follicles of the TA group were dense and mature, indicating that trans-anisole has the effect of promoting ovarian development. Compared with the ZEA group, the ovarian surface follicles of the ZEA×TA group were more, larger, and more mature, indicating that trans-anisole alleviated the toxic effect of zearalenone on the ovaries.

[0114] 3.8.4 Other organ indices

[0115] The effects of zearalenone and trans-anisole on other organ indexes of weaned piglets are shown in Table 9. 1.5 mg / kg zearalenone and 500 mg / kg trans-anisole had no significant effect on other organ indexes of weaned piglets (P>0.05). There was no significant interaction between zearalenone and trans-anisole on other organ indexes of piglets (P>0.05).

[0116] Table 9 Effects of zearalenone and trans-anisole on other organ indexes of weaned piglets (g / kg, n=6)

[0117]

[0118] Note: CON: control; ZEA: zearalenone; TA: trans-anisole.

[0119] 4. Summary

[0120] Zearalenone is a mycotoxin that has the effect of estrogen and can increase the estrogen level of piglets. The experiment of the present invention confirms that feeding feed contaminated with zearalenone to piglets can cause the uterine organ index to increase and the ovarian organ index to decrease, which has obvious damage to the reproductive organs of piglets. When trans-anisole is applied to the feed of piglets contaminated with zearalenone for detoxification, it can be observed that the uterine organ index decreases significantly and the ovarian organ index increases significantly. It shows that trans-anisole has the effect of alleviating the reproductive toxicity of zearalenone to piglets.

[0121] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. The application of trans-anisole in the preparation of drugs for alleviating zearalenone poisoning in animals.

2. The use according to claim 1, characterized in that: The animals include piglets.

3. Application of trans-anisole in the preparation of feed for alleviating zearalenone poisoning in animals.

4. The use according to claim 3, characterized in that: The animals include piglets.

5. A drug for alleviating zearalenone poisoning in animals, characterized in that: The main component of the medicine is trans-anisole.

6. The drug according to claim 5, characterized in that The medicine also contains pharmaceutically acceptable auxiliary materials.

7. The drug according to claim 5, characterized in that The animals include piglets.

8. A feed for alleviating animal zearalenone poisoning, characterized in that: The feed contains trans-anisole.

9. The feed according to claim 8, characterized in that The added concentration of trans-anisole in the feed is 500 mg / kg.

10. The feed according to claim 8, characterized in that The animals include piglets.

Images