New application of swine bacillus velezensis

Abstract

The invention provides a novel application of swine bacillus velezensis, the content of the swine bacillus velezensis in a freeze-drying preparation is 1 * 10 < 9 > CFU / g, the related swine bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is classified and named as Bacillus velezensis, and the preservation number is CGMCC No.32057. The swine bacillus velezensis freeze-drying preparation has the advantages that the swine bacillus velezensis freeze-drying preparation can be used for preparing the swine bacillus velezensis freeze-drying preparation; according to the invention, piglets and broiler chickens infected with vomitoxin are taken as experimental animals, the excavated swine bacillus is added into feed, and the effect of the swine bacillus on the diseased piglets and broiler chickens is observed, so that the bacterial strain is found to have the effect of remarkably reducing intestinal injuries of the piglets and broiler chickens caused by vomitoxin, and also can improve the antioxidant capacity of intestinal tracts of the diseased piglets and broiler chickens. The experimental results show that the strain has the capability of treating vomitoxin poisoning of livestock and poultry.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms and livestock and poultry breeding, and in particular to a new application of pig-derived Bacillus Velezii. Background Art

[0002] Deoxynivalenol (DON), also known as vomitoxin, is a trichothecenes toxin widely found in cereal crops such as wheat, barley, and corn. Livestock and poultry that eat feed made from contaminated grains will experience symptoms of gastroenteritis such as nausea, vomiting, diarrhea, anorexia, and gastrointestinal bleeding, leading to decreased feed intake and reduced daily weight gain. Among livestock and poultry, pigs are the most sensitive to DON. Vomitoxin mainly affects protein synthesis by affecting the synthesis of DNA and RNA and blocking the initiation of translation. Most of its toxic effects originate from this mechanism. The effects of vomitoxin on animals are mainly concentrated in the digestive tract and kidneys, which can cause diffuse necrosis of the digestive tract, hemorrhage, erythropenia, poor coagulation, severe dermatitis, decreased immunity, decreased egg production, slower hematopoietic regeneration in the bone marrow and spleen, lesions in the reproductive organs, and necrosis and hemorrhage of tissues such as the testicles and ovaries. Infected animals often experience symptoms such as weight loss, poor feed utilization, bloody diarrhea, and miscarriage, and severe cases can lead to death.

[0003] At present, in order to prevent the impact of vomitoxin on livestock and poultry in advance, some breeders use traditional physical and chemical methods to remove vomitoxin from feed. Although it has a certain effect, there are safety issues such as incomplete detoxification and introduction of new pollutants, and the cost of detoxification is high. Some breeders use biological detoxification to remove vomitoxin from feed. Biological detoxification refers to the method of using microorganisms or biological enzymes to convert toxins into non-toxic or low-toxic metabolites. It is currently considered to be the most ideal detoxification method, but this method can only solve problems such as high vomitoxin content in food, agricultural and sideline products, and feed, and mycotoxin contamination in agriculture and food. For livestock and poultry that have been infected with vomitoxin, most of them still use antibiotic therapy, which not only increases the risk of excessive antibiotics in livestock and poultry meat, but also the effect is often unsatisfactory.

[0004] There is currently no effective treatment for vomitoxin poisoning in livestock and poultry. Summary of the invention

[0005] The purpose of the present invention is to solve the problem that there is currently no effective method for treating vomitoxin poisoning in livestock and poultry, and further provide the use of porcine Bacillus Velezii in alleviating vomitoxin poisoning in piglets.

[0006] In order to achieve the above technical objectives, the technical solutions adopted in this application are as follows: In a first aspect, the present invention provides a porcine Bacillus Velezii freeze-dried preparation, wherein the content of porcine Bacillus Velezii in the freeze-dried preparation is 1×109 CFU / g, and the porcine Bacillus Velezii is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

[0007] In a second aspect, the present invention provides a method for preparing the lyophilized preparation according to the first aspect, comprising the following steps: Step 1, activating and culturing the porcine-derived Bacillus Velez subtilis in a culture medium for multiple generations, and then performing a first amplification culture; Step 2, performing a second amplification culture on the bacterial solution obtained by the first amplification culture in step 1; Step 3, collecting the bacterial cells in the bacterial solution cultured for the second time in step 2 and preparing a bacterial suspension; Step 4, adding a protective agent to the bacterial suspension obtained in step 3 and then freeze-drying the mixture to obtain the freeze-dried preparation.

[0008] Preferably, in step 1, the porcine Bacillus Velez subtilis is activated and cultured in a culture medium for 3 generations; and / or, the first amplification culture is carried out in LB broth medium with shaking culture at 37° C. for 10 to 14 hours; and / or, In step 2, the second amplification culture is to inoculate the bacterial solution of the first amplification culture in step 1 into LB broth medium at an inoculum of 1-3%, and ferment and culture at 35-39° C. for 20-30 hours; and / or, In step 3, the bacterial suspension is prepared using sterile physiological saline; and / or, In step 4, the protective agent is skim milk powder. Preferably, the amount of skim milk powder added is 8-12% of the mass of the bacterial suspension obtained in step 3.

[0009] In a third aspect, the present invention provides a use of a porcine Bacillus Velezii or the freeze-dried preparation described in the first aspect in the preparation of a product for preventing and treating vomitoxin poisoning in livestock and poultry. The porcine Bacillus Velezii is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

[0010] In a fourth aspect, the present invention provides a use of a porcine Bacillus Velezii or the lyophilized preparation described in the first aspect in the preparation of a product for repairing intestinal oxidative damage caused by vomitoxin poisoning in livestock and poultry. The porcine Bacillus Velezii is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

[0011] Preferably, the product is one of feed additives, feed, medicine, and health products.

[0012] In a fifth aspect, the present invention provides a product for preventing and treating vomitoxin poisoning in livestock and poultry, wherein the product contains porcine Bacillus Velezii, which is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

[0013] Preferably, the mass content of porcine-derived Bacillus Velez subtilis in the product is 1-2%.

[0014] In a sixth aspect, the present invention provides a product for repairing intestinal oxidative damage caused by vomitoxin poisoning in livestock and poultry, wherein the product contains porcine Bacillus Velezii, which is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

[0015] Preferably, the mass content of porcine-derived Bacillus Velez subtilis in the product is 1-2%.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention uses piglets and broilers infected with vomitoxin as experimental animals, adds the discovered porcine Bacillus to feed and observes its effect on sick piglets and broilers, and finds that the strain has the effect of significantly reducing the intestinal damage of piglets and broilers caused by vomitoxin, and can also improve the antioxidant capacity of the intestines of sick piglets and broilers. These experimental results show that the strain has the ability to treat vomitoxin poisoning in livestock and poultry. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the growth status of the swine-derived Bacillus YB-X-733 obtained in Example 1 of the present invention.

[0018] Figure 2 The staining results of the porcine Bacillus YB-X-733 in Example 2 of the present invention are shown in Figure A, wherein Figure A is the Gram staining result and Figure B is the spore staining result.

[0019] Figure 3 This is the genetic evolution tree of the swine-derived Bacillus YB-X-733 in Example 2 of the present invention.

[0020] Figure 4 This is the growth curve of the porcine Bacillus YB-X-733 in Example 2 of the present invention. DETAILED DESCRIPTION

[0021] The porcine Bacillus Velezii used in the present invention is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

[0022] In the description of the present invention, it should be noted that, if the specific conditions are not specified in the examples, the experiments were carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially.

[0023] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments, which are intended to explain the present invention rather than to limit it. Example 1

[0024] The screening and separation of swine-derived Bacillus is as follows: Take the fresh feces of healthy adult antibiotic-free Mianyang local pigs as the sample, aseptically weigh 1 g of feces sample, put it into 10 mL of sterile PBS buffer, shake it to mix it thoroughly, place it in a 90℃ water bath for 10 minutes to kill other non-spore bacteria, then dilute it with sterile water, take an appropriate amount of the dilution and spread it on a soybean casein agar (TSA) plate, place it in a 37℃ incubator, invert and culture it for 48 hours, observe the growth of the plate colony, pick a single colony and streak it on a new plate, add LB medium, and culture it aerobically at 37℃, pH=7.0±0.1 for 8 hours. Figure 1 As shown, a Bacillus strain was finally screened and isolated, numbered YB-X-733, and submitted to the General Microbiology Center of China Microbiological Culture Collection Administration for preservation.

[0025] The pig-derived Bacillus obtained by the above screening was studied and identified for its relevant characteristics, as follows: (1) Antagonistic properties of candidate swine-derived Bacillus against pathogens Experimental method: After the LB broth solid medium is sterilized and cooled to about 50 ℃, it is poured into a sterile culture dish. After the plate is naturally dried, 0.1 mL of indicator bacteria solution (10 8 CFU / mL) was evenly spread on the plate, and after natural drying, a hole was punched on the plate, and 0.1 mL of the test bacterial solution YB-X-733 was added to the hole. After the bacterial solution penetrated into the plate, it was cultured at 37 °C for 24 h, and the size of the inhibition zone was measured. The results showed that YB-X-733 had a good inhibitory effect on Escherichia coli, Staphylococcus aureus and Salmonella. The antibacterial results are shown in Table 1.

[0026] Table 1 Results of antibacterial test of candidate bacteria

[0027] (2) Screening of acid and bile salt resistance of porcine Bacillus (2-1) Bile salt tolerance test The bacteria to be tested were inoculated into LB liquid culture medium. After culturing for 12-24 h, 5% of the bacterial solution was inoculated into LB liquid culture medium with different bile salt levels (0.15% and 0.3%) and placed at 37°C for 2 h or 4 h. 100 μL of the bacterial solution was diluted 10-fold at 0, 2, and 4 h, and 100 μL of the bacterial solution was diluted 10-fold at 0, 2, and 4 h. -5 The gradient dilutions were spread on LB plates, cultured at 37°C for 24 h, and colonies were counted. Each sample was replicated 3 times. The survival rate was calculated as follows: Survival rate (%) = number of colonies at “2 h or 4 h” / number of colonies at “0 h” × 100%.

[0028] (2-2) Acid resistance test The bacteria to be tested were inoculated into LB liquid culture medium. After culturing for 12-24 h, 5% of the bacterial solution was inoculated into LB liquid culture medium with a pH of 2 or 3 and placed at 37 °C for 2 h or 4 h. 100 μL of the bacterial solution was diluted 10-fold at 0 and 2 h, and 10 -5 The gradient dilutions were spread on LB plates, cultured at 37°C for 24 h, and colonies were counted. Each sample was replicated 3 times. The survival rate was calculated as follows: Survival rate (%) = number of colonies at “2 h or 4 h” / number of colonies at “0 h” × 100%.

[0029] The test results are shown in Table 2. The results show that strain YB-X-733 has strong acid and bile salt resistance.

[0030] Table 2 Acid and bile salt tolerance of candidate strains

[0031] (3) Morphological identification of swine-derived Bacillus YB-X-733 The colony morphology of the strain is shown in Table 3. Gram staining observation showed that the strain was a Gram-positive bacillus, and both Gram staining and spore staining were positive ( Figure 2 The morphological characteristics of YB-X-733 colonies are shown in Table 3.

[0032] Table 3 Morphological characteristics of YB-X-733 colonies

[0033] (4) Identification of physiological and biochemical characteristics of strain YB-X-721 The physiological and biochemical characteristics of strain YB-X-721 are shown in Table 4. The comparative analysis was carried out with reference to the Manual of Identification of Common Bacterial Systems and the Bergey's Manual of Identification of Bacteria, which was consistent with the physiological and biochemical characteristics of Bacillus.

[0034] Table 4 Physiological and biochemical identification results of YB-X-721

[0035] Note: + indicates positive; - indicates negative.

[0036] (5) Molecular biological identification of strain YB-X-721 The strain was sequenced for 16S gene, and the sequencing result is shown in SEQ ID NO.1. BLAST comparison was performed in GenBank to draw a genetic evolution tree. The results showed that YB-X-733 was similar to the reference strain. Bacillus velezensis On the same branch, the affinity is very high ( Figure 3 ), so it can be determined that YB-X-733 is Bacillus subtilis and named Bacillus velezensis YB-X-733.

[0037] SEQ ID NO.1:

[0038] (6) Analysis of biological characteristics of YB-X-733 strain After the strain was activated for one generation, it was inoculated into 100 mL LB liquid culture medium at a 2% (V / V) inoculation rate and cultured at 37°C with shaking. 5 mL of the bacterial solution was taken for testing every 2 hours, and the concentration (OD value) of the bacterial solution was measured at a wavelength of 600 nm using an ultraviolet spectrophotometer for a total of 14 hours.

[0039] By measuring the growth curve of the porcine Bacillus Velezii YB-X-733, the growth curve is as follows Figure 4 As shown, it can be found that YB-X-721 entered the logarithmic growth phase after 2 h and entered the stable phase after 8 h. Example 2

[0040] The application of swine-derived Bacillus YB-X-733 in alleviating vomitoxin poisoning in piglets is as follows: 1) Test method: The weaned Rongchang piglets were randomly divided into 4 groups according to their weight, with 3 replicates in each group and 4 weaned Rongchang piglets in each replicate. The 4 groups were: control group (fed with a basic diet for 21 days); DON poisoning group (fed with a basic diet of 1.5 mg / kg DON for 14 days, and all piglets showed symptoms of vomitoxin poisoning, and then continued to be fed with a basic diet for 7 days); Bacillus YB-X-733 treatment group (fed with a basic diet of 1.5 mg / kg DON for 14 days, and all piglets showed symptoms of vomitoxin poisoning, and then continued to be fed with a diet supplemented with 1×10 7 CFU / g Velez Bacillus YB-X-733 in the basal diet for 7 days, with the addition of Velez Bacillus YB-X-733 being 1% of the basal diet); the commercial Bacillus treatment group (fed with a basal diet of 1.5 mg / kg DON for 14 days, after all piglets showed symptoms of vomitoxin poisoning, they continued to be fed with 1×10 7 CFU / g of commercial Bacillus (cultivated Suda Bacillus subtilis, Huisheng Biological) in the basic diet for 7 days, and the amount of commercial Bacillus added was 1% of the basic diet). The feeding cycle was 21 days.

[0041] 2) Effect of YB-X-733 on the growth performance of piglets poisoned by vomitoxin Table 5 shows the growth performance of weaned piglets in each group. As can be seen from Table 5, there was no difference in initial weight among the groups, and the weights of the piglets in each group were relatively close. After 21 days of feeding, the weight, average daily weight gain and feed-to-weight ratio of the DON group were significantly lower than those of the control group ( P<0.05), while the addition of Bacillus YB-X-733 significantly alleviated the decrease in these indicators and achieved the effect of commercial probiotic Bacillus. The results showed that the addition of Bacillus can significantly alleviate the inhibitory effect of vomitoxin on the growth performance of piglets, and the effect is comparable to that of veterinary Bacillus on the market.

[0042] Table 5 Effect of YB-X-733 on growth performance of weaned piglets

[0043] Note: Different letters in the same row represent significant differences (P<0.05). The same below.

[0044] 3) Effect of dietary supplementation with Bacillus sp. YB-X-733 on intestinal development in piglets poisoned by vomitoxin The main function of the villi of the small intestine is to absorb nutrients. The increase in the height of the intestinal villi can increase the area of ​​the small intestine to absorb nutrients. When the villi become shorter, the number of intestinal epithelial cells decreases, and the ability to digest and absorb nutrients is also reduced. The depth of the crypt mainly reflects the generation rate of epithelial cells. Epithelial cells continuously migrate and differentiate from the base of the crypt to the end of the villi to form villus cells with absorption capacity to supplement the normally shed villus cells. If this process slows down, the cell generation rate at the base of the crypt decreases, making the crypt shallower. Therefore, the shallowing of the crypt indicates that the maturation rate of intestinal epithelial cells has increased and the intestinal secretory function has been strengthened.

[0045] As shown in Table 6, the small intestine of piglets was significantly damaged after vomitoxin poisoning, as shown by a significant decrease in the height of the small intestinal villi in the DON group (P<0.05) and a significant deepening of the crypt depth (P<0.05). The addition of Bacillus YB-X-733 can significantly alleviate the changes in intestinal morphological damage caused by vomitoxin poisoning, and the height of the intestinal villi in the duodenum, jejunum, and ileum is significantly increased compared with the poisoning group (P<0.05). At the same time, compared with the addition of commercial Bacillus on the market, the recovery of the intestinal villus height by the strain YB-X-733 is more significant. This shows that Bacillus YB-X-733 can significantly reduce the intestinal damage of vomitoxin on weaned piglets to maintain intestinal health, and its effect of alleviating damage is slightly better than that of commercial probiotic Bacillus.

[0046] Table 6 Effects of YB-X-733 on intestinal development of weaned piglets

[0047] Note: Data in the same industry that do not contain the same lowercase letters indicate significant differences ( P <0.05, the same letters or no letters indicate no significant difference ( P >0.05); the same as in the table below.

[0048] 4) Effect of dietary supplementation with Bacillus YB-X-733 on intestinal antioxidant capacity of weaned Rongchang piglets Biochemical indices related to oxidative stress in the small intestinal mucosa were detected, among which superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were indicators of antioxidant capacity, and malondialdehyde (MDA) was an indicator of oxidative damage.

[0049] The activities of SOD and GSH-PX in the small intestinal mucosa of piglets in the DON group were significantly lower than those in the control group (P<0.05), while the content of MDA in the small intestinal mucosa was significantly higher than that in the control group (P<0.05). After adding Bacillus YB-X-733, the effects of vomitoxin on these indicators were significantly alleviated (P<0.05), and the alleviation effect was slightly better than that of adding commercial probiotic Bacillus. This shows that Bacillus YB-X-733 improves the antioxidant capacity of the intestine of weaned piglets and reduces the oxidative damage of vomitoxin to the intestine of piglets. The data are shown in Table 7.

[0050] Table 7 Effects of YB-X-733 on antioxidant enzyme activities and MDA content in small intestinal mucosa of weaned piglets

[0051] Example 3 Application of swine-derived Bacillus YB-X-733 in alleviating vomitoxin poisoning in broiler chickens At present, the contamination of vomitoxin in feed has a great impact on the intestinal health and immune performance of poultry, and even affects the feed intake and production performance of poultry. This example carried out the application research of swine-derived Bacillus YB-X-733 in broiler vomitoxin poisoning.

[0052] A total of 120 healthy 1-day-old Avian (AA) broiler chickens of similar weight were selected and randomly divided into 4 groups, with 6 replicates in each group and 5 chickens in each replicate. The control group (fed with a basic diet for 21 days, the basic diet was a powdered feed prepared according to the nutritional requirements of NRC (1994)); the DON poisoning group (fed with a basic diet containing 5 mg / kg DON for 14 days, after all broilers showed symptoms of DON poisoning, they were fed a basic diet without DON for the last 7 days); the Bacillus YB-X-733 treatment group (fed with a basic diet containing 5 mg / kg DON for 14 days, after all broilers showed symptoms of DON poisoning, they were fed a diet supplemented with 1×10 7 CFU / g Bacillus YB-X-733 for 7 days); Commercial Bacillus treatment group (fed with a basic diet containing 5 mg / kg DON for 14 days, after all broilers showed symptoms of DON poisoning, they were fed a diet supplemented with 1×10 7CFU / g commercial Bacillus (same as Example 2) for 7 days. The experimental period was 21 days. The broilers were fed ad libitum and had plenty of drinking water. They were immunized according to the normal immunization procedure.

[0053] 1. Effect of YB-X-733 on body weight changes of broilers poisoned by vomitoxin

[0054] Table 4 shows the weight changes of broiler chickens in each group. As shown in Table 8, the weight of the DON poisoning group was significantly lower than that of the control group ( P <0.05), while the addition of Bacillus YB-X-733 significantly alleviated the decrease in these indicators and achieved the effect of commercial probiotic Bacillus. The results showed that the addition of Bacillus can significantly alleviate the inhibitory effect of vomitoxin on the growth performance of broiler chickens, and its effect is comparable to that of veterinary Bacillus available on the market.

[0055] Table 8 Body weight changes of broilers

[0056] Note: Different letters in the same row represent significant differences (P<0.05). The same below.

[0057] 2. Effect of dietary supplementation with Bacillus sp. YB-X-733 on intestinal development in broiler chickens poisoned by vomitoxin

[0058] As shown in Table 9, the small intestine of broilers poisoned by DON showed obvious damage, as shown by the significant decrease in the height of the small intestinal villi in the DON poisoning group (P<0.05) and the significant deepening of the crypt depth (P<0.05). The addition of Bacillus YB-X-733 can significantly alleviate the changes in intestinal morphological damage caused by DON poisoning, and the height of the intestinal villi in the duodenum, jejunum, and ileum is significantly increased compared with the poisoning group (P<0.05). This shows that Bacillus YB-X-733 can significantly reduce the intestinal damage of DON on broilers to maintain intestinal health, and its effect in alleviating damage is slightly better than that of commercial probiotic Bacillus.

[0059] Table 9 Effects of YB-X-733 on intestinal development of broiler chickens

[0060] Note: Data in the same industry that do not contain the same lowercase letters indicate significant differences ( P <0.05, the same letters or no letters indicate no significant difference ( P >0.05); the same as in the table below.

[0061] 3. Effect of dietary supplementation with Bacillus subtilis YB-X-733 on intestinal antioxidant capacity of broiler chickens

[0062] The activities of SOD and GSH-PX in the small intestinal mucosa of broiler chickens in the DON poisoning group were significantly lower than those in the control group (P<0.05), while the MDA content in the small intestinal mucosa was significantly higher than that in the control group (P<0.05). After adding Bacillus YB-X-733, the effects of vomitoxin on these indicators were significantly alleviated (P<0.05), and the alleviation effect reached the level of adding commercial probiotic Bacillus. This shows that Bacillus YB-X-733 improves the antioxidant capacity of the small intestine of broiler chickens and reduces the oxidative damage of vomitoxin to the small intestine of broiler chickens. The data are shown in Table 10.

[0063] Table 10 Effects of YB-X-733 on antioxidant enzyme activities and MDA content in small intestinal mucosa of weaned piglets

[0064] In summary, the above data on the effect of this porcine Bacillus on piglets and broilers infected with vomitoxin prove that this strain has the effect of significantly reducing intestinal damage caused by vomitoxin and can also improve the antioxidant capacity of the intestine. This strain is expected to be further developed into a product for the treatment of vomitoxin poisoning in livestock and poultry, with huge commercial value and market potential.

[0065] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A freeze-dried preparation of porcine Bacillus Velezii, characterized in that: The content of porcine Bacillus Velezii in the freeze-dried preparation is 1×10 9 CFU / g, the pig-derived Bacillus Velez is classified and named as Bacillus Velez ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the collection date is September 24, 2024, and the collection number is CGMCC No.32057.

2. The method for preparing the freeze-dried preparation according to claim 1, characterized in that: The steps include: Step 1, activating and culturing the porcine-derived Bacillus Velez subtilis in a culture medium for multiple generations, and then performing a first amplification culture; Step 2, performing a second amplification culture on the bacterial solution obtained by the first amplification culture in step 1; Step 3, collecting the bacterial cells in the bacterial solution cultured for the second time in step 2 and preparing a bacterial suspension; Step 4, adding a protective agent to the bacterial suspension obtained in step 3 and then freeze-drying the mixture to obtain the freeze-dried preparation.

3. The preparation method according to claim 2, characterized in that: In the step 1, the porcine Bacillus Velez subtilis is activated and cultured in a culture medium for 3 generations; and / or, the first amplification culture is carried out in an LB broth medium with shaking culture at 37° C. for 10 to 14 hours; and / or, In step 2, the second amplification culture is to inoculate the bacterial solution of the first amplification culture in step 1 into LB broth medium at an inoculum of 1-3%, and ferment and culture at 35-39° C. for 20-30 hours; and / or, In step 3, the bacterial suspension is prepared using sterile physiological saline; and / or, In step 4, the protective agent is skim milk powder. Preferably, the amount of skim milk powder added is 8-12% of the mass of the bacterial suspension obtained in step 3.

4. Use of the porcine Bacillus Velezii or the freeze-dried preparation according to claim 1 in the preparation of a product for preventing and treating vomitoxin poisoning in livestock and poultry, wherein the porcine Bacillus Velezii is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

5. Use of the porcine Bacillus Velez or the freeze-dried preparation according to claim 1 in the preparation of a product for repairing intestinal oxidative damage caused by vomitoxin poisoning in livestock and poultry, wherein the porcine Bacillus Velez is classified and named as Bacillus Velez ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

6. The use according to claim 3 or 4, characterized in that: The product is one of feed additives, feed, medicine and health products.

7. A product for preventing and treating vomitoxin poisoning in livestock and poultry, characterized in that: The product contains porcine Bacillus Velezii, which is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 24, 2024, and the preservation number is CGMCC No.32057.

8. The product for preventing and treating vomitoxin poisoning in livestock and poultry according to claim 6, characterized in that: The mass content of porcine Bacillus Velezii in the product is 1~2%.

9. A product for repairing intestinal oxidative damage caused by vomitoxin poisoning in livestock and poultry, characterized in that: The product contains porcine Bacillus Velezii, which is classified and named as Bacillus Velezii ( Bacillus velezensis ), deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, the address of the collection center is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the collection date is September 24, 2024, and the collection number is CGMCC No.32057.

10. The product for repairing intestinal oxidative damage caused by vomitoxin poisoning in livestock and poultry according to claim 6, characterized in that: The mass content of porcine Bacillus Velezii in the product is 1~2%.

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