Shuangsheng black soil as well as preparation method and application thereof

Abstract

The invention provides gemini black soil as well as a preparation method and application thereof. Belongs to the technical field of black soil regeneration. According to the method, the independently researched and developed multifunctional matrix rod fermentation system is utilized, and the cross-species crossed double-biological combined food chain is combined to decompose the silkworm waste plants, so that the waste plants are converted into regenerated black soil within an extremely short time, and the target of converting the humus black soil characteristic soil of the waste plants for 14-17 days is achieved; the efficiency and benefits of industrial black soil production are greatly improved, the problem of waste plant and straw treatment becomes simple and easy, and the method is beneficial for rapidly repairing degenerated black soil, increasing the biological activity of the soil and increasing the soil fertility, fertility, organic matter content and organic carbon content.

Description

Technical Field

[0001] The invention relates to the technical field of regenerated black soil, and more specifically to twin black soil and a preparation method and application thereof. Background Art

[0002] China has 1.8 billion mu of cultivated land, of which 520 million mu is black soil, accounting for 28% of the total cultivated land. Black soil is a unique treasure given by nature to mankind. It is a clay soil with strong swelling, contraction and disturbance characteristics. It has good properties and high fertility, making it very suitable for plant growth. Natural black soil forms slowly. It takes 300-400 years for a 1 cm black soil layer to complete ecological cycle and natural accumulation. Even general abandoned plants need at least 1-2 years to naturally turn into humus black soil. With the rapid development of agriculture, people's over-exploitation of black soil has intensified the trend of continuous reduction of high-quality cultivated land and black soil. Excessive reclamation and cultivation, large-scale application of chemical fertilizers, abnormal climate and other causes of soil erosion, soil degradation, sharp reduction of organic matter, and thinning, thinning and hardening of black soil are becoming increasingly serious. Curbing the degradation of black soil and quickly repairing and conserving black soil have attracted great attention from the society.

[0003] Since black soil in nature is located in the frigid and temperate zones, it is difficult to artificially transform black soil and produce black soil using biological methods under low temperatures. At present, there is no public and mature industrial method for artificially regenerating humus black soil at home and abroad.

[0004] Therefore, how to develop a method for preparing regenerated humus black soil with lower raw material cost, greater safety, rapidity and high efficiency is a technical problem that technical personnel in this field urgently need to solve. Summary of the invention

[0005] In view of this, the present invention provides a dual-biological black soil and a preparation method and application thereof. With the help of dual biological action and advanced treatment technology, the waste plant material is converted into regenerated black soil in a very short time, achieving the goal of converting the waste plant humus into black soil characteristic soil within 14-17 days. It is conducive to the rapid repair of the degraded black soil.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A method for preparing twin black soil comprises the following steps:

[0008] (1) Low-temperature fermentation: Plant waste is crushed and added to a multifunctional matrix rod fermentation system, black soil inducing bacteria and low-temperature fermentation bacteria are injected, and the fermentation temperature is controlled within the range of 35-40°C through the heat recovery effect of the matrix rods. Low-temperature fermentation is performed for 72 hours to obtain low-temperature fermentation materials;

[0009] (2) High temperature fermentation: injecting high temperature fermentation bacteria and air into the low temperature fermentation material through the multifunctional matrix rod fermentation system, controlling the temperature at 70-75°C, and high temperature fermentation for 24 hours to obtain the high temperature fermentation material;

[0010] (3) Fermentation at low temperature again: adjust the temperature to 35-40° C. by heat recovery of the matrix rods, inject black soil inducing bacteria and low temperature fermentation bacteria into the obtained high temperature fermentation material, ferment at low temperature for 72 hours, and adjust the material temperature to 28° C. by heat recovery of the matrix rods again for standby use;

[0011] (4) Bi-biological culture: After injecting black soil inducing bacteria, low-temperature fermentation bacteria and air into the material obtained in step (3) through a multifunctional matrix rod fermentation system, the material is transferred to a culture bed, and low-temperature-resistant and saprophytic earthworms are added. Under the action of the bi-biological agents, the material is cultured at 15-28°C for 7-10 days, and the earthworms and soil are separated to obtain regenerated black soil.

[0012] Preferably, the composition of the black soil inducing bacteria in steps (1), (3) and (4) is: 35% Aspergillus niger and 65% Bacillus subtilis; the composition of the low-temperature fermentation bacteria is: 25% Rhizobium, 45% Acetobacter striatus, 20% lactic acid bacteria and 10% Methanosarcina.

[0013] Preferably, the plant waste in step (1) includes but is not limited to plant stalks, straw, discarded tree branches and leaves, wormwood, weeds and wood chips.

[0014] Preferably, the crushing particle size of the plant waste in step (1) is 100-200 mesh; the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 20% and 80% respectively; calculated based on the plant waste with a humidity of 40%, 0.5L of the mixed bacterial solution of black soil inducing bacteria and low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8cfu / ml.

[0015] Preferably, the high-temperature fermentation bacteria in step (2) include thermophiles and / or high-temperature actinomycetes, and the amount of high-temperature fermentation bacteria added is calculated based on the material with a humidity of 40%, and 0.5 L of high-temperature fermentation bacteria liquid is added per ton, and the concentration of the high-temperature fermentation bacteria liquid is 10^8 cfu / ml; the amount of air added is calculated based on the material with a humidity of 40%, and 20 cubic meters of fresh air is added per ton.

[0016] Preferably, in step (3), the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 30% and 70% respectively; calculated based on the material with a humidity of 40%, 0.5 L of the mixed bacterial solution of the black soil inducing bacteria and the low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8 cfu / ml.

[0017] Preferably, in step (4), the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 20% and 80% respectively. Calculated based on the material with a humidity of 40%, 0.5 L of the mixed bacterial solution of the black soil inducing bacteria and the low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8 cfu / ml.

[0018] Preferably, the amount of low-temperature-resistant saprophilous earthworms added in step (4) is not less than 10% of the total amount of the material; the amount of air added is calculated based on the material with 40% humidity, and 20 cubic meters of fresh air is added per ton.

[0019] Another object of the present invention is to provide: application of the above method in preparing twin black soil.

[0020] Another object of the present invention is to provide: the twin black soil prepared by the above method.

[0021] Another object of the present invention is to provide: application of the above-mentioned twin black soil in soil improvement and crop cultivation.

[0022] It can be seen from the above technical solution that compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The present invention utilizes a self-developed multifunctional matrix rod fermentation system, and combines it with a cross-species dual-organism combination food chain to decompose and erode waste plants, so that the waste plants are converted into regenerated black soil in a very short time, achieving the goal of converting 15-20d waste plant humus into black soil characteristic soil, greatly improving the efficiency and benefits of industrial black soil production, making the problem of waste plant and straw treatment simple and easy, and facilitating the rapid repair of degraded black soil, increasing soil biological activity, soil fertility, fertility, organic matter, and organic carbon content.

[0024] (2) It avoids a series of environmental problems such as carbon dioxide emissions and air pollution caused by the burning of abandoned plants and straw; and fills the gap in the black soil industry supply chain caused by the inability to trade black soil after the implementation of the Black Soil Protection Law.

[0025] (3) In the decomposition and transformation of waste plants into black soil by the microbial flora in the dual organisms, the black soil inducing bacteria play a role in establishing the organic carbon source structure of the black soil and producing black humic acid; the low-temperature fermentation bacteria decompose the waste plant materials to provide edible nutrients for earthworms, and are catalysts that assist in the digestion and decomposition of food in the food chain of scavenging earthworms. The low-temperature tolerant scavenging grass earthworms are a mixture of more than two species of earthworms. The mixed treatment of two kinds of earthworms to decompose the waste plant materials catalyzed by low-temperature bacteria can stimulate the territorial attributes of earthworms, accelerate their food grabbing, and improve the feeding efficiency of earthworms. The two kinds of earthworms engulf the materials catalyzed by the low-temperature bacteria and excrete a large amount of substances rich in humic acid, black humic acid, organic carbon, etc. After the earthworms engulf and digest them in the intestinal cavity, they excrete humus, mucus, mineral colloids, and organic colloids to form the granular structure of earthworm manure black soil. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0027] Figure 1 For: method flow chart;

[0028] Figure 2 For: the plan view of the heat preservation fermentation tank;

[0029] Figure 3 : A top view of the heat preservation fermentation tank;

[0030] Figure 4 For: a three-dimensional cross-sectional view of a heat preservation fermentation tank;

[0031] Figure 5 For: Matrix rod disassembly and assembly diagram;

[0032] Figure 6 This is a diagram of an integrated cultivation and harvesting device;

[0033] Figure 7 This is: the device diagram of light-sensing earthworm-repelling equipment;

[0034] Figure 2-Figure 5 Among them, 1-insulated fermentation tank, 2-opening for material inlet and outlet, 3-pit seat, 4-matrix rod lifting landing gear, 5-lifting zipper, 6-matrix rod, 7-water inlet pipe, 8-water outlet pipe, 9-liquid supply, gas pipe, 61-shell, 62-internal plug-in end cover, 63-bottom cover, 64-heat absorbing and heat transfer copper pipe, 65-heat absorbing and heat transfer copper pipe water inlet, 66-heat absorbing and heat transfer copper pipe water outlet, 67-interface for installation of heat absorbing and heat transfer copper pipe connecting pipe, 68-heat absorbing and heat transfer copper pipe connecting pipe, 69-liquid supply, gas pipe connection hole, 70-gas outlet, liquid outlet hole, 71-suspension ring, 72-heat absorbing and heat transfer copper pipe fixing hoop and 73-sensing control system;

[0035] Figure 6-Figure 7 Among them, 1-harvesting equipment, 2-light-sensing earthworm-driving equipment, 3-connecting rod, 4-culture bed, 11-harvesting box, 12-harvesting impeller, 13-roller conveyor belt, 14-transmission auger, 15-lifting device, 21-light source, 22-roller cage, 23-axle head seat, 24-bearing, 25-optical fiber column, 41-bed frame, 42-trough body, 43-track, 44-track wheel. DETAILED DESCRIPTION

[0036] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0037] The two devices, the multifunctional matrix rod fermentation system and the integrated culture and collection device used in the embodiments of the present invention, are devices independently developed by the inventor, and invention patents were applied for on the same day as the present application. The corresponding patent names are: a multifunctional matrix rod fermentation system and application; a light-sensing earthworm machine interactive separation and harvesting equipment, system and application.

[0038] The composition of the black soil inducing bacteria used in Examples 1-3 is: 35% Aspergillus niger and 65% Bacillus subtilis; the composition of the low-temperature fermentation bacteria is: 25% Rhizobium, 45% Acetobacter striatus, 20% Lactobacillus and 10% Methanosarcina; the high-temperature fermentation bacteria include thermophiles and / or thermophilic actinomycetes.

[0039] Example 1

[0040] A method for preparing twin black soil comprises the following steps:

[0041] (1) Low-temperature fermentation: 1 ton of plant waste was crushed to 200 mesh, the material humidity was adjusted to 40%, and added to the multifunctional matrix rod fermentation system. 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80% was injected, and the bacterial solution concentration was 10^8 cfu / ml. The temperature was controlled at 40°C through the heat recovery effect of the matrix rod, and the low-temperature fermentation was performed for 72 hours to obtain a low-temperature fermentation material;

[0042] (2) High-temperature fermentation: 0.5 L of high-temperature actinomycetes is injected into the low-temperature fermentation material through a multifunctional matrix rod fermentation system, the bacterial liquid concentration is 10^8 cfu / ml, and 20 cubic meters of fresh air is injected at the same time. The temperature is controlled at 75°C and the high-temperature fermentation is performed for 24 hours to obtain the high-temperature fermentation material;

[0043] (3) Low-temperature fermentation again: The temperature was controlled at 40°C by heat recovery of the matrix rods, and 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 30% and 70% was injected into the high-temperature fermentation material. The bacterial solution concentration was 10^8 cfu / ml, and the low-temperature fermentation was performed for 72 h. The material temperature was adjusted to 28°C by heat recovery of the matrix rods again and set aside;

[0044] (4) Double biological culture: 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80% was injected into the material obtained in step (3) through a multifunctional matrix rod fermentation system, and the bacterial solution concentration was 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air was injected, and the material was transferred to a culture bed, and 10% of the total weight of the material was added. Under the action of the double biological, the material was placed at 15°C for 10 days, and the earthworms were separated from the earthworms by a light-sensing earthworm machine interactive separation and harvesting device to obtain regenerated black soil ( Figure 1 ).

[0045] In this embodiment, steps (1) to (3) are performed using a multifunctional matrix rod fermentation system ( Figure 2-Figure 5 ), the plant waste material is subjected to low temperature-high temperature-low temperature fermentation treatment, the system comprises: a heat preservation fermentation tank and matrix rods, the heat preservation fermentation tank is provided with a plurality of pit seats flush with the ground, and the matrix rods can be vertically installed in the pit seats;

[0046] The multifunctional matrix rod fermentation system is equipped with a sensor control system. During the fermentation process, the flow of water medium in the heat-absorbing and heat-transferring copper tube of the matrix rod is controlled to export or pour in bio-heat energy, thereby realizing the recovery of bio-heat energy during the fermentation process and temperature control of the fermentation materials. The fermentation time can be shortened by 24 hours, which saves energy and improves work efficiency.

[0047] At the same time, the matrix rod is also provided with connection holes for liquid supply and gas supply pipes and outlets of multiple liquid supply and gas supply holes at different heights on the surface of the matrix rod.

[0048] When the multifunctional matrix rod fermentation system is running, the plant waste to be fermented is placed in the heat preservation fermentation tank. Under the instruction of the temperature sensor sensing signal, the heat-absorbing heat transfer copper tube exports the microbial heat energy through the water medium, or imports the reserved heat energy to make the plant waste ferment quickly at the optimal temperature. At this time, the matrix rod plays the role of heat recovery of microbial energy and temperature control and maintenance of fermentation materials. At the same time, through the matrix rod liquid supply and gas supply passage, the waste plant materials in the fermentation tanks of different depths are aerated, water is injected to adjust humidity, the bacterial solution is replaced, and the temperature is adjusted. All fermentation processes are completed in one station, without the need for complex processes such as flipping, overturning, repeated throwing of bacterial solution, and multiple stirring of traditional fermentation processes, reducing the investment in production equipment such as flipping machines, mixers, ventilation and cooling systems, saving equipment investment funds, greatly shortening the fermentation time and improving work efficiency.

[0049] The dual biological culture and separation and collection in step (4) of this embodiment is carried out using an integrated culture and collection device ( Figure 6-Figure 7 ):

[0050] The culture bed used includes: a bed frame, a trough body and a track, wherein the trough body is fixed on the bed frame; the track is two tubular tracks, which are arranged above the bed frame and the trough body and are formed integrally with the bed frame; the light-sensing earthworm machine interactive separation and harvesting equipment moves along the track above the trough body to separate and harvest the twin black soil;

[0051] The light-sensing earthworm machine interactive separation and harvesting equipment used includes a harvesting device and a light-sensing earthworm driving device. The harvesting device is located behind the light-sensing earthworm driving device and is connected to the light-sensing earthworm driving device through a connecting rod.

[0052] The light-sensitive earthworm-repelling device is provided with a rolling cage, an axle head seat, a bearing and a light source; the axle head seat and the bearing are provided at the center of the circular cross section of the rolling cage, so that the rolling cage passes through the bearing and rolls around the axle head seat as the center;

[0053] The light source is arranged inside the rolling cage; a light column light source is arranged on the outer wall of the rolling cage;

[0054] The light source is 100-200W 760nm light lamp;

[0055] The harvesting equipment includes a harvesting box, a harvesting impeller, a roller conveyor belt and a conveying auger; the harvesting impeller, the roller conveyor belt and the conveying auger are connected to each other, the harvesting impeller transfers the harvested materials to the roller conveyor belt, and the conveying auger transfers the harvested materials to the harvesting box.

[0056] When the light-sensing earthworm-driving interactive separation and harvesting equipment is running, the harvesting equipment runs on the track at a uniform speed. The light-sensing earthworm-driving equipment located in front of the machine body and running synchronously is placed on the black soil of the trough body and rolls forward. During the movement, the nano-light lamp in the rolling cage is lit. The light emitted by the 760-nanometer spectrum has extremely strong penetration, which is also the most sensitive spectrum range for earthworms. The light is introduced into the soil layer through the optical fiber column. The soil layer is 3-5cm deep and has light perception. After the earthworms sense it, they will actively drill down to avoid it due to stress response. When the soil-digging impeller moves to the light-sensing earthworm-driving rolling cage passing area, the earthworms have drilled under the 3mm soil layer. During the movement of the harvesting impeller, 3cm thick soil is removed and transmitted to the synchronously running harvesting box. The earthworm-driving interactive separation and harvesting equipment will move 3cm down to the soil layer each time it reciprocates. When it reaches the earthworm avoidance limit, the earthworm-driving interactive separation and harvesting equipment will stop working and continue to add new materials to the culture bed to carry out the next cycle of dual biological culture. In this way, the natural separation of earthworms and soil can be achieved without harming the earthworms, and the earthworms can be recycled many times.

[0057] Example 2

[0058] A method for preparing twin black soil comprises the following steps:

[0059] (1) Low-temperature fermentation: 1 ton of corn stalks were crushed into 100 mesh, the material humidity was adjusted to 40%, and added to the multifunctional matrix rod fermentation system. 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80% was injected, and the bacterial solution concentration was 10^8 cfu / ml. The temperature was controlled at 35°C through the heat recovery effect of the matrix rods, and the low-temperature fermentation was performed for 72 hours to obtain a low-temperature fermentation material;

[0060] (2) High temperature fermentation: 0.5 L of thermophilic bacteria was injected into the low temperature fermentation material through the multifunctional matrix rod fermentation system, and the bacterial liquid concentration was 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air was injected, and the temperature was controlled at 70°C. The high temperature fermentation was performed for 24 hours to obtain the high temperature fermentation material;

[0061] (3) Low-temperature fermentation again: The temperature was controlled at 35°C by heat recovery of the matrix rods, and 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 30% and 70% was injected into the high-temperature fermentation material. The bacterial solution concentration was 10^8 cfu / ml, and the low-temperature fermentation was performed for 72 h. The material temperature was adjusted to 28°C by heat recovery of the matrix rods again and set aside;

[0062] (4) Double biological culture: 0.5L of mixed bacterial solution consisting of black soil inducing bacteria and low temperature fermentation bacteria in a ratio of 20% and 80% is injected into the material obtained in step (3) through a multifunctional matrix rod fermentation system, and the bacterial solution concentration is 10^8cfu / ml. At the same time, 20 cubic meters of fresh air is injected, and the material is transferred to a culture bed, and 10% of the total weight of the material is added. Eisenia fetida and E. heterolipis are placed at 15-28°C for 7-10 days under the action of the double biologicals, and the earthworms are separated from the earthworms by a light-sensing earthworm machine interactive separation and harvesting device to obtain regenerated black soil. The equipment used in this embodiment is the same as that in Example 1.

[0063] The effective components of the twin black soil prepared in Example 2 were determined, and the experimental results are as follows: the organic matter content was 119 g / kg, the total nitrogen content was 0.513%, the total phosphorus content was 1.92 g / kg, and the total potassium content was 2.13%.

[0064] Example 3

[0065] A method for preparing twin black soil comprises the following steps:

[0066] (1) Low-temperature fermentation: 1 ton of soybean straw was crushed into 150 meshes, the material humidity was adjusted to 40%, and added to the multifunctional matrix rod fermentation system. 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80% was injected, and the bacterial solution concentration was 10^8 cfu / ml. The temperature was controlled at 37°C through the heat recovery effect of the matrix rod, and the low-temperature fermentation was performed for 72 hours to obtain a low-temperature fermentation material;

[0067] (2) High temperature fermentation: 0.5 L of thermophilic bacteria was injected into the low temperature fermentation material through the multifunctional matrix rod fermentation system, and the bacterial liquid concentration was 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air was injected, and the temperature was controlled at 73°C. The high temperature fermentation was performed for 24 hours to obtain the high temperature fermentation material;

[0068] (3) Low-temperature fermentation again: The temperature was controlled at 38°C by heat recovery of the matrix rods, and 0.5 L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 30% and 70% was injected into the high-temperature fermentation material. The bacterial solution concentration was 10^8 cfu / ml, and the low-temperature fermentation was performed for 72 h. The material temperature was adjusted to 28°C by heat recovery of the matrix rods again and set aside;

[0069] (4) Double biological culture: 0.5L of mixed bacterial solution consisting of black soil inducing bacteria and low temperature fermentation bacteria in a ratio of 20% and 80% is injected into the material obtained in step (3) through a multifunctional matrix rod fermentation system, and the bacterial solution concentration is 10^8cfu / ml. At the same time, 20 cubic meters of fresh air is injected, and the material is transferred to a culture bed, and 10% of the total weight of the material is added. Eisenia fetida and E. heterolipis are placed at 15-28°C for 7-10 days under the action of the double biologicals, and the earthworms are separated from the earthworms by a light-sensing earthworm machine interactive separation and harvesting device to obtain regenerated black soil. The equipment used in this embodiment is the same as that in Example 1.

[0070] The effective components of the twin black soil prepared in Example 3 were determined, and the experimental results are as follows: the organic matter content was 380 g / kg, the total nitrogen content was 1.426%, the total phosphorus content was 2.71 g / kg, and the total potassium content was 1.52%.

[0071] In the second national soil survey soil nutrient classification standard, soil with organic matter>4% meets the first-level soil standard. From Examples 2 and 3, it can be seen that the organic matter content of the regenerated black soil prepared by the dual-biomass method of the present invention is 2.9-9.5 times higher than the minimum standard of the first-level soil.

[0072] The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0073] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing twin black soil, characterized in that: The steps include: (1) Low-temperature fermentation: Plant waste is crushed and added to a multifunctional matrix rod fermentation system, black soil inducing bacteria and low-temperature fermentation bacteria are injected, and the fermentation temperature is controlled within the range of 35-40°C through the heat recovery effect of the matrix rods. Low-temperature fermentation is performed for 72 hours to obtain low-temperature fermentation materials; (2) High temperature fermentation: injecting high temperature fermentation bacteria and air into the low temperature fermentation material through the multifunctional matrix rod fermentation system, controlling the temperature at 70-75°C, and high temperature fermentation for 24 hours to obtain the high temperature fermentation material; (3) Fermentation at low temperature again: adjust the temperature to 35-40° C. by heat recovery of the matrix rods, inject black soil inducing bacteria and low temperature fermentation bacteria into the obtained high temperature fermentation material, ferment at low temperature for 72 hours, and adjust the material temperature to 28° C. by heat recovery of the matrix rods again for standby use; (4) Bi-biological culture: After injecting black soil inducing bacteria, low-temperature fermentation bacteria and air into the material obtained in step (3) through a multifunctional matrix rod fermentation system, the material is transferred to a culture bed, and low-temperature-resistant and saprophytic earthworms are added. Under the action of the bi-biological agents, the material is cultured at 15-28°C for 7-10 days, and the earthworms and soil are separated to obtain regenerated black soil.

2. The method for preparing the twin black soil according to claim 1, characterized in that: The composition of the black soil inducing bacteria in steps (1), (3) and (4) is: 35% Aspergillus niger and 65% Bacillus subtilis; the composition of the low-temperature fermentation bacteria is: 25% Rhizobium, 45% Acetobacter striatus, 20% Lactobacillus and 10% Methanosarcina.

3. The method for preparing the twin black soil according to claim 1, characterized in that: The crushed particle size of the plant waste in step (1) is 100-200 mesh; the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 20% and 80% respectively; calculated based on the plant waste with a humidity of 40%, 0.5L of a mixed bacterial solution of black soil inducing bacteria and low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8cfu / ml.

4. The method for preparing the twin black soil according to claim 1, characterized in that: The high-temperature fermentation bacteria in step (2) include thermophilic bacteria and / or high-temperature actinomycetes. The amount of high-temperature fermentation bacteria added is calculated based on the material with a humidity of 40%, and 0.5L of high-temperature fermentation bacteria liquid is added per ton. The concentration of the high-temperature fermentation bacteria liquid is 10^8cfu / ml. The amount of air added is calculated based on the material with a humidity of 40%, and 20 cubic meters of fresh air is added per ton.

5. The method for preparing the twin black soil according to claim 1, characterized in that: In step (3), the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 30% and 70% respectively. Calculated based on the material with a humidity of 40%, 0.5L of the mixed bacterial solution of the black soil inducing bacteria and the low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8cfu / ml.

6. The method for preparing the twin black soil according to claim 1, characterized in that: In step (4), the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 20% and 80% respectively. Calculated based on the material with a humidity of 40%, 0.5L of the mixed bacterial solution of the black soil inducing bacteria and the low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8cfu / ml.

7. The method for preparing the twin black soil according to claim 1, characterized in that: The amount of low-temperature-resistant saprophilous earthworms added in step (4) is not less than 10% of the total weight of the material; the amount of air added is calculated based on the material with 40% humidity, and 20 cubic meters of fresh air is added per ton.

8. Use of the method according to any one of claims 1 to 7 in preparing twin black soil.

9. Gemini black soil prepared by the method according to any one of claims 1 to 7.

10. Use of the twin black soil according to claim 9 in soil improvement and crop cultivation.

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