Antioxidant whitening and repairing component based on fission yeast fermentation technology and preparation method of antioxidant whitening and repairing component

Abstract

The invention relates to the technical field of cosmetics and skin care products, and discloses an antioxidant whitening and repairing component based on a fission yeast fermentation technology, which comprises the following components by mass percent: 5-15% of fission yeast fermentation extract; 2%-6% of nicotinamide; 1%-4% of ethyl ascorbic acid; 0.5% to 2% of a ceramide compound; 0.5% to 1.5% of sodium hyaluronate; 0.1% to 0.5% of beta-glucan; 0.1% to 0.5% of a hexapeptide; 1%-5% of glycerin; 40%-80% of water; 0.5%-2% of a fermented white truffle extract; 0.5%-1.5% of crithmum maritimum stem cell extract; and 0.5%-1.5% of phospholipid. By adding the fission yeast fermentation extract and cooperating with nicotinamide and ethyl ascorbic acid, melanin generation can be effectively inhibited, uneven skin color is improved, and the moisturizing performance is enhanced by a ceramide compound and sodium hyaluronate in the water-phase component.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics and skin care products, and in particular to an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology and a preparation method thereof. Background Art

[0002] "Whitening" has always been a constant theme in the beauty market, and there has always been a huge market demand. Whitening, freckle removal, freckle removal, yellowing removal, and melanin removal have become the main demands for beauty whitening functions. The depth of skin color is regulated by many factors, mainly depending on the content and distribution of melanin. Melanin is an amino acid derivative, which is formed by a series of chemical reactions of tyrosine or 3,4-dihydroxyphenylalanine, and it often exists in a polymerized form.

[0003] Melanin is synthesized by melanocytes in the basal layer of the human epidermis. When ultraviolet rays are irradiated on the skin, the skin will be in a state of "self-protection", activating the activity of tyrosinase, catalyzing the oxidation of tyrosine or 3,4-dihydroxyphenylalanine to produce melanin to protect our skin cells. Melanin moves through layers of cell metabolism and reaches the epidermis of the skin to form freckles, sun spots, dark spots, etc.

[0004] In the prior art, traditional whitening products mainly rely on ingredients such as niacinamide and vitamin C derivatives. Although these ingredients have certain whitening and antioxidant effects, they are easily oxidized or inactivated during product storage and use, thus affecting the final effect. At the same time, traditional antioxidant ingredients have limited permeability, and they have more effects on the surface of the skin, but have weaker effects deep into the skin, making it difficult to achieve the purpose of deep repair. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology and a preparation method thereof, which solves the problems of unstable activity, insufficient permeability and poor moisturizing effect of whitening and antioxidant ingredients in existing skin care products.

[0006] To achieve the above objectives, the present invention is implemented by the following technical scheme: an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology, comprising the following mass percentages:

[0007] 5%-15% fission yeast fermentation extract;

[0008] 2%-6% niacinamide;

[0009] 1%-4% ethyl ascorbic acid;

[0010] 0.5%-2% ceramide complex;

[0011] 0.5%-1.5% sodium hyaluronate;

[0012] 0.1%-0.5% beta-glucan;

[0013] 0.1%-0.5% hexapeptide;

[0014] 1%-5% glycerol;

[0015] 40%-80% water;

[0016] 0.5%-2% fermented white truffle extract;

[0017] 0.5%-1.5% sea fennel stem cell extract;

[0018] 0.5%-1.5% phospholipids;

[0019] 0.5%-2.5% plant squalane;

[0020] 0.1%-0.5% chamomile extract;

[0021] 0.01%-0.1% lavender essential oil.

[0022] Preferably, the preparation steps of the fission yeast fermentation extract are:

[0023] Inoculate the fission yeast strain into the culture medium;

[0024] Cultivate in a fermenter, adjust the initial pH value of the culture medium to -6.0-7.0, set the temperature to 35°C-40°C, introduce oxygen, the oxygen flow rate is 0.5vvm-1.5vvm, the fermentation time is 24H-72H, monitor the OD600 value of the fermentation liquid during this period, and keep the bacterial concentration at 1OD-5OD;

[0025] After the fermentation is completed, the fermentation liquid is centrifuged at a speed of 2000 rpm to 6000 rpm and at 0°C to 10°C for 10 min to 30 min to obtain a supernatant;

[0026] The supernatant after centrifugation is filtered through a 5kDa-15kDa ultrafiltration membrane to separate the macromolecular components, and the diafiltrate containing small molecular peptides, amino acids and antioxidant enzymes is collected;

[0027] The diafiltrate is subjected to liquid-liquid extraction, and ethyl acetate having the same volume as the fermentation broth is added, and the fat-soluble components are allowed to enter the organic phase under stirring conditions, and the organic phase containing polyphenols is separated;

[0028] The aqueous phase was concentrated by rotary evaporation to 1 / 5-1 / 20 of the original volume, the temperature was controlled at 30°C-40°C, and then vacuum freeze-dried at -10°C--50°C to obtain the fission yeast fermentation extract.

[0029] A method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology, comprising the following steps:

[0030] S1. Inoculating the fission yeast strain into a culture medium containing glucose, peptone and yeast extract, and obtaining a fission yeast fermentation extract after culturing, centrifugation, ultrafiltration and drying;

[0031] S2, inoculating white truffle strains into a culture medium containing glucose, malt extract and amino acids, and obtaining a fermented white truffle extract by fermentation, ultrafiltration and drying;

[0032] S3, preparing an aqueous phase: adding fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerol into water and dissolving them to obtain an aqueous phase;

[0033] S4, preparing an oil phase: mixing plant squalane, phospholipids, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil to obtain an oil phase;

[0034] S5. Slowly add the oil phase into the water phase and prepare a uniform emulsion after emulsification.

[0035] Preferably, the emulsification step in step S5 is:

[0036] S51, stirring at a speed of 3000 rpm-8000 rpm for 5 min-10 min;

[0037] S52, use 20kHz-40kHz ultrasonic treatment for 10min-20min.

[0038] Preferably, the culture medium in step S1 contains 5g / L-30g / L glucose, 5g / L-15g / L peptone and 3g / L-10g / L yeast extract, the pH value is adjusted within the range of 6.0-7.0, and the culture is carried out at 35°C-40°C for 24H-72H.

[0039] Preferably, the preparation of the fermented white truffle extract in step S2 comprises the following steps:

[0040] S21, inoculating the white truffle strain into a culture medium containing 5g / L-20g / L glucose, 3g / L-10g / L malt extract and 0.5g / L-5g / L amino acids, controlling the temperature at 25°C-30°C, and fermenting for 48H-96H;

[0041] S22, after the fermentation is completed, filtering through a 5kDa-15kDa ultrafiltration membrane to obtain a filtrate;

[0042] S23. Freeze-drying the filtrate to obtain a fermented white truffle extract.

[0043] Preferably, in step S3, the aqueous phase material is stirred at room temperature at a speed of 500 rpm-1500 rpm for 15 min-30 min.

[0044] Preferably, the oil phase materials in step S4 are mixed at a temperature of 40° C.-60° C.

[0045] Preferably, the mass ratio of the water phase to the oil phase is 1:1-1:4.

[0046] Preferably, the S23 freeze-drying temperature is -10°C to 50°C.

[0047] The present invention provides an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology and a preparation method thereof. It has the following beneficial effects:

[0048] 1. The present invention can effectively inhibit melanin production, improve uneven skin tone, and make the skin more translucent by adding fission yeast fermentation extract and cooperating with niacinamide and ethyl ascorbic acid. The ceramide complex and sodium hyaluronate in the water phase components enhance the moisturizing performance and improve the skin barrier function. The addition of glycerol further locks in moisture, thereby significantly improving the moisturizing effect of the product.

[0049] 2. The present invention adopts the fermentation and extraction process of fission yeast and white truffle, retaining active substances such as polypeptides, amino acids and antioxidant enzymes. These ingredients work together in the product to bring efficient repair effects and antioxidant properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] Figure 1 It is a step diagram of the preparation method of the present invention. DETAILED DESCRIPTION

[0051] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0052] Embodiment 1:

[0053] Please see attached Figure 1 The embodiment of the present invention provides an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology, including the following mass percentages:

[0054] 5%-15% fission yeast fermented extract; 2%-6% niacinamide; 1%-4% ethyl ascorbic acid; 0.5%-2% ceramide complex; 0.5%-1.5% sodium hyaluronate; 0.1%-0.5% beta-glucan; 0.1%-0.5% hexapeptide; 1%-5% glycerin; 40%-80% water; 0.5%-2% fermented white truffle extract; 0.5%-1.5% sea fennel stem cell extract; 0.5%-1.5% phospholipids; 0.5%-2.5% plant squalane; 0.1%-0.5% chamomile extract; 0.01%-0.1% lavender essential oil.

[0055] Specifically, the fission yeast fermentation extract is rich in peptides, amino acids, antioxidant enzymes and other ingredients, which can repair the skin barrier, retain moisture, provide antioxidant protection, and effectively resist free radical damage. Its fermentation active ingredients work synergistically with niacinamide and ethyl ascorbic acid to bring more significant whitening and antioxidant effects.

[0056] Niacinamide has anti-inflammatory and whitening effects. It can inhibit the transfer of melanin to the stratum corneum, improve uneven skin tone, and enhance the skin barrier function.

[0057] Ethyl ascorbic acid is a stable vitamin C derivative with a strong antioxidant effect. It can brighten the skin, reduce spots, and has excellent antioxidant properties, helping the skin fight oxidative stress.

[0058] Ceramide complex and sodium hyaluronate work together to form a matrix that moisturizes and repairs the skin barrier, replenishes skin lipids, and reduces water loss, thereby maintaining skin softness and elasticity.

[0059] Among β-glucan and hexapeptide, β-glucan has anti-inflammatory and immunomodulatory effects, helping to soothe the skin, while hexapeptide can promote collagen synthesis, improving skin firmness and elasticity. Fermented white truffle extract and sea fennel stem cell extract: White truffle is rich in a variety of antioxidant ingredients, and sea fennel stem cell extract can promote skin cell repair and regeneration. Plant squalane, phospholipids, chamomile extract and lavender essential oil: These oil phase ingredients provide soothing, nourishing and anti-inflammatory effects to the skin, while enhancing the stability of the emulsion.

[0060] The preparation steps of fission yeast fermentation extract are as follows:

[0061] Inoculate the fission yeast strain into the culture medium;

[0062] Cultivate in a fermenter, adjust the initial pH value of the culture medium to -6.0-7.0, set the temperature to 35°C-40°C, introduce oxygen, the oxygen flow rate is 0.5vvm-1.5vvm, the fermentation time is 24H-72H, monitor the OD600 value of the fermentation liquid during this period, and keep the bacterial concentration at 1OD-5OD;

[0063] After the fermentation is completed, the fermentation liquid is centrifuged at a speed of 2000 rpm to 6000 rpm and at 0°C to 10°C for 10 min to 30 min to obtain a supernatant;

[0064] The supernatant after centrifugation is filtered through a 5kDa-15kDa ultrafiltration membrane to separate the macromolecular components, and the diafiltrate containing small molecular peptides, amino acids and antioxidant enzymes is collected;

[0065] The diafiltrate is subjected to liquid-liquid extraction, and ethyl acetate having the same volume as the fermentation broth is added, and the fat-soluble components are allowed to enter the organic phase under stirring conditions, and the organic phase containing polyphenols is separated;

[0066] The aqueous phase was concentrated by rotary evaporation to 1 / 5-1 / 20 of the original volume, the temperature was controlled at 30°C-40°C, and then vacuum freeze-dried at -10°C--50°C to obtain the fission yeast fermentation extract.

[0067] Specifically, through proper oxygen supply and temperature control, fission yeast can grow under optimal conditions and produce more bioactive substances, such as peptides and antioxidant enzymes. Regulation of OD600 helps control the concentration of bacteria and ensure the stability of the fermentation process. During the process, low-temperature centrifugation can maintain the stability of active ingredients and remove large molecular impurities, while ultrafiltration membranes can effectively separate useful small molecule nutrients to ensure the purity and activity of the extract. In addition, fat-soluble components are extracted by ethyl acetate, and after rotary evaporation and freeze-drying, the activity of antioxidant components is retained to the maximum extent, making fission yeast fermentation extract more suitable for skin care applications, bringing excellent antioxidant and moisturizing effects.

[0068] A method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology, comprising the following steps:

[0069] S1. Inoculating the fission yeast strain into a culture medium containing glucose, peptone and yeast extract, and obtaining a fission yeast fermentation extract after culturing, centrifugation, ultrafiltration and drying;

[0070] The culture medium in step S1 contains 5g / L-30g / L glucose, 5g / L-15g / L peptone and 3g / L-10g / L yeast extract, the pH value is adjusted within the range of 6.0-7.0, and the culture is carried out at 35°C-40°C for 24H-72H.

[0071] S2, inoculating white truffle strains into a culture medium containing glucose, malt extract and amino acids, and obtaining a fermented white truffle extract by fermentation, ultrafiltration and drying;

[0072] The preparation comprises the following steps:

[0073] S21, inoculating the white truffle strain into a culture medium containing 5g / L-20g / L glucose, 3g / L-10g / L malt extract and 0.5g / L-5g / L amino acids, controlling the temperature at 25°C-30°C, and fermenting for 48H-96H;

[0074] S22, after the fermentation is completed, filtering through a 5kDa-15kDa ultrafiltration membrane to obtain a filtrate;

[0075] S23. Freeze-dry the filtrate at a temperature of -10°C to 50°C to obtain a fermented white truffle extract.

[0076] Specifically, when fermented under suitable low-temperature conditions, white truffles produce rich polyphenols, amino acids and antioxidants, and have strong antioxidant activity.

[0077] S3, preparing an aqueous phase: adding fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerol into water and dissolving them to obtain an aqueous phase; in step S3, the aqueous phase material is stirred at room temperature at a speed of 500 rpm-1500 rpm for 15 min-30 min.

[0078] S4. Prepare the oil phase: mix plant squalane, phospholipids, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil to obtain an oil phase; maintain 40° C.-60° C. during mixing.

[0079] S5. Slowly add the oil phase into the water phase and prepare a uniform emulsion after emulsification. The emulsification steps are as follows:

[0080] S51, stirring at a speed of 3000 rpm-8000 rpm for 5 min-10 min;

[0081] S52, use 20kHz-40kHz ultrasonic treatment for 10min-20min.

[0082] The mass ratio of the water phase to the oil phase is 1:1-1:4.

[0083] Example 2: Moderate doses of fission yeast fermented extract and fermented white truffle extract

[0084] Based on Example 1, the present invention provides an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology and a preparation method thereof, including the following mass percentages:

[0085] Aqueous phase:

[0086] Fission yeast fermentation extract: 10%

[0087] Niacinamide: 4%

[0088] Ethyl ascorbic acid: 3%

[0089] Ceramide Complex: 1%

[0090] Sodium Hyaluronate: 1%

[0091] Beta-glucan: 0.3%

[0092] Hexapeptide: 0.3%

[0093] Glycerin: 3%

[0094] Water: 66.4%

[0095] Oil phase:

[0096] Fermented white truffle extract: 1%

[0097] Sea fennel stem cell extract: 1%

[0098] Phospholipids: 1%

[0099] Plant Squalane: 1.5%

[0100] Chamomile extract: 0.3%

[0101] Lavender essential oil: 0.05%

[0102] Preparation steps:

[0103] Preparation of aqueous phase: Add fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerol into water and stir at 1000 rpm for 20 minutes until dissolved.

[0104] Oil phase preparation: Mix plant squalane, phospholipids, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil at 50°C until uniform.

[0105] Emulsification: The oil phase was slowly added to the water phase, stirred at 5000 rpm for 8 minutes, and then treated with 30 kHz ultrasound for 15 minutes to obtain a uniform emulsion.

[0106] Summary: This formula uses a moderate dose of fission yeast fermented extract and fermented white truffle extract. This dose can provide good antioxidant and whitening effects. The proportion of fission yeast fermented extract in the formula can significantly improve the moisturizing effect without causing stickiness, while maintaining the stability of the lotion.

[0107] The standard ultrasonic emulsification process ensures uniform distribution of the oil phase and the water phase, and good stability. Tests show that this embodiment has outstanding performance in moisturizing and anti-oxidation, and has a significant whitening effect.

[0108] Example 3: Low-dose fission yeast fermentation extract and fermented white truffle extract

[0109] Based on Example 1, the present invention provides an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology and a preparation method thereof, including the following mass percentages:

[0110] Aqueous phase:

[0111] Fission yeast ferment extract: 4%

[0112] Niacinamide: 1.5%

[0113] Ethyl ascorbic acid: 0.8%

[0114] Ceramide Complex: 0.2%

[0115] Sodium hyaluronate: 0.3%

[0116] Beta-glucan: 0.05%

[0117] Hexapeptide: 0.05%

[0118] Glycerin: 1%

[0119] Water: 91.1%

[0120] Oil phase:

[0121] Fermented white truffle extract: 0.4%

[0122] Sea fennel stem cell extract: 0.3%

[0123] Phospholipids: 0.3%

[0124] Plant Squalane: 0.8%

[0125] Chamomile extract: 0.05%

[0126] Lavender essential oil: 0.01%

[0127] Preparation steps:

[0128] Preparation of aqueous phase: Add fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerol into water and stir at 600 rpm for 15 minutes at room temperature until dissolved.

[0129] Oil phase preparation: Mix plant squalane, phospholipids, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil at 40°C until uniform.

[0130] Emulsification: The oil phase was slowly added to the water phase, stirred at 3000 rpm for 5 min, and then treated with 20 kHz ultrasound for 10 min to obtain a uniform emulsion.

[0131] Summary: Low doses of fission yeast fermented extract and fermented white truffle extract are used in this formula, which reduces the overall cost of the formula. The emulsification stirring rate and ultrasonic frequency are also adjusted down accordingly, so the moisturizing, whitening and anti-oxidation effects are not as good as those in Example 1, but it is still better than some skin care products without active ingredients on the market. Lower doses of antioxidant ingredients can meet daily skin care needs and are suitable for users who have cost requirements.

[0132] Example 4: High-dose fission yeast fermented extract and fermented white truffle extract

[0133] Based on Example 1, the present invention provides an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology and a preparation method thereof, including the following mass percentages:

[0134] Aqueous phase:

[0135] Fission yeast ferment extract: 15%

[0136] Niacinamide: 6%

[0137] Ethyl ascorbic acid: 4%

[0138] Ceramide Complex: 2%

[0139] Sodium hyaluronate: 1.5%

[0140] Beta-glucan: 0.5%

[0141] Hexapeptide: 0.5%

[0142] Glycerin: 5%

[0143] Water: 55%

[0144] Oil phase:

[0145] Fermented white truffle extract: 2%

[0146] Sea fennel stem cell extract: 1.5%

[0147] Phospholipids: 1.5%

[0148] Plant Squalane: 2.5%

[0149] Chamomile extract: 0.5%

[0150] Lavender essential oil: 0.1%

[0151] Preparation steps:

[0152] Preparation of aqueous phase: Add fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerol into water and stir at 1200 rpm for 30 minutes until completely dissolved.

[0153] Oil phase preparation: Mix plant squalane, phospholipids, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil at 60°C until uniform.

[0154] Emulsification: The oil phase was slowly added to the water phase, stirred at 8000 rpm for 10 min, and then treated with 40 kHz ultrasound for 20 min to obtain a uniform emulsion.

[0155] Summary: This formula uses high-dose fission yeast fermented extract and fermented white truffle extract, and adopts an efficient emulsification process, which makes the product have significant effects in anti-oxidation, whitening, moisturizing, etc. The high concentration of fission yeast fermented extract and fermented white truffle extract further enhances the skin repair and antioxidant effects, and the inhibitory effect on melanin formation is also very obvious. This high-dose product is suitable for users who pursue significant skin care effects.

[0156] Summary

[0157] In Examples 2 to 4, the high flexibility and formulation optimization capability of the present invention are demonstrated through ingredient formulations with different dosages and different process parameter tests.

[0158] Example 2 shows a balance between high cost performance and effect, and is suitable for daily skin care;

[0159] Embodiment 3 focuses on meeting the economic requirements and still has excellent effects under cost control;

[0160] Example 4 provides significant whitening and antioxidant effects and is suitable for the high-end skin care market.

[0161] In general, the present invention can optimize the effect by adjusting the formula and process parameters, and is suitable for users with different levels of needs.

[0162] Test experiment

[0163] The skin moisturizing, whitening, antioxidant activity and 30-day stability tests were conducted. The results are as follows:

[0164]

[0165] Comparative experiment

[0166] Comparative experiment 1: lack of fission yeast fermentation extract and fermented white truffle extract; recipe:

[0167] Niacinamide: 4%;

[0168] Ethyl ascorbic acid: 3%;

[0169] Ceramide complex: 1%;

[0170] Sodium hyaluronate: 1%;

[0171] Glycerol: 3%;

[0172] Plant squalane: 1.5%;

[0173] Water: 86.5%;

[0174] Comparative experiment 2: low-dose innovative ingredients but no phacoemulsification;

[0175] Formula and process:

[0176] Fission yeast fermentation extract: 4%;

[0177] Fermented white truffle extract: 0.4%;

[0178] The other ingredients are the same as in Example 2;

[0179] Emulsification: mechanical stirring at 3000 rpm for 10 minutes without ultrasonic treatment;

[0180] Comparative experiment 3: mechanical emulsification only;

[0181] The formula is the same as in Example 2, but the process is different:

[0182] Emulsification: mechanical stirring at 5000 rpm for 10 minutes without ultrasonic treatment;

[0183] The experimental data table is as follows:

[0184]

[0185] Comparative experimental results analysis

[0186] The comparative experiment 1 lacking fission yeast fermented extract and fermented white truffle extract showed that the moisturizing and antioxidant properties were significantly reduced, and the stability was poor, indicating that the innovative ingredients play a key role in moisturizing, antioxidant and stability.

[0187] A comparative experiment with low doses of innovative ingredients and no phacoemulsification2 showed that phacoemulsification helped to improve the uniform distribution of ingredients, enhance antioxidant properties, and significantly enhance stability and avoid stratification.

[0188] Comparative Experiment 3 with only mechanical emulsification was still inferior to Example 2 in terms of antioxidant activity and stability, indicating that ultrasonic emulsification improved the uniformity of the emulsion and the permeability of the ingredients, thereby enhancing the overall efficacy.

[0189] Test experiment

[0190] Test Experiment A: Conventional skin care products without active extracts (basic formula)

[0191] formula:

[0192] Niacinamide: 4%;

[0193] Ethyl ascorbic acid: 3%;

[0194] Ceramide complex: 1%;

[0195] Sodium hyaluronate: 1%;

[0196] Glycerol: 3%;

[0197] Plant squalane: 1.5%;

[0198] Chamomile extract: 0.3%;

[0199] Lavender essential oil: 0.05%;

[0200] Water: 86.15%;

[0201] Preparation process:

[0202] Preparation of aqueous phase: Add niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate and glycerin into water and stir until dissolved to obtain an aqueous phase.

[0203] Preparation of oil phase: Mix plant squalane, chamomile extract and lavender essential oil, stir evenly to obtain an oil phase.

[0204] Emulsification: The oil phase was slowly added to the water phase and stirred at 5000 rpm for 10 min without ultrasonic treatment to obtain a basic emulsion.

[0205] Test Experiment B: Skin Care Products Without Fission Yeast Fermentation Extract

[0206] formula:

[0207] Niacinamide: 4%;

[0208] Ethyl ascorbic acid: 3%;

[0209] Ceramide complex: 1%;

[0210] Sodium hyaluronate: 1%;

[0211] β-Glucan: 0.3%;

[0212] Hexapeptide: 0.3%;

[0213] Glycerol: 3%;

[0214] Fermented white truffle extract: 1%;

[0215] Sea fennel stem cell extract: 1%;

[0216] Plant squalane: 1.5%;

[0217] Chamomile extract: 0.3%;

[0218] Lavender essential oil: 0.05%;

[0219] Water: 83.55%;

[0220] Preparation process:

[0221] Preparation of aqueous phase: Add niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerin into water and stir to dissolve.

[0222] Oil phase preparation: Mix plant squalane, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil at 50°C.

[0223] Emulsification: The oil phase was slowly added to the water phase, stirred at 5000 rpm for 8 min, and then treated with 30 kHz ultrasound for 15 min to obtain a uniform emulsion.

[0224] Test Experiment C: Skin Care Products Without Fermented White Truffle Extract

[0225] formula:

[0226] Fission yeast fermentation extract: 10%;

[0227] Niacinamide: 4%;

[0228] Ethyl ascorbic acid: 3%;

[0229] Ceramide complex: 1%;

[0230] Sodium hyaluronate: 1%;

[0231] β-Glucan: 0.3%;

[0232] Hexapeptide: 0.3%;

[0233] Glycerol: 3%;

[0234] Sea fennel stem cell extract: 1%;

[0235] Plant squalane: 1.5%;

[0236] Chamomile extract: 0.3%;

[0237] Lavender essential oil: 0.05%;

[0238] Water: 74.55%;

[0239] Preparation process:

[0240] Preparation of aqueous phase: Add fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerin into water and stir to dissolve.

[0241] Oil phase preparation: Mix plant squalane, sea fennel stem cell extract, chamomile extract and lavender essential oil at 50°C.

[0242] Emulsification: The oil phase was slowly added to the water phase, stirred at 5000 rpm for 8 min, and then treated with 30 kHz ultrasound for 15 min to obtain a uniform emulsion.

[0243] Test data of embodiments and test experiments

[0244] Each example (Examples 2-4) and test experiment (AC) will be tested for moisturizing, whitening, antioxidant activity and stability.

[0245]

[0246] Experimental results analysis

[0247] Comparison with traditional skin care products without extracts (Test A):

[0248] Test experiment A lacks fission yeast fermentation extract and fermented white truffle extract, and only contains basic moisturizing ingredients such as niacinamide, ethyl ascorbic acid, ceramide complex and sodium hyaluronate. The moisturizing, whitening and antioxidant effects are significantly lower than the embodiments of the present invention. The moisturizing degree is only 55%, the whitening and enhancement effect (L* value) is only 3.5, and the antioxidant activity is only 35%. The stratification phenomenon is obvious and the stability is poor. The results show that products lacking fission yeast fermentation extract and fermented white truffle extract are difficult to achieve the skin care effect of the present invention and are prone to stratification.

[0249] Comparison with the formulation lacking fission yeast fermentation extract (Test Experiment B):

[0250] Test experiment B did not contain fission yeast fermentation extract, but contained fermented white truffle extract. Compared with test experiment A, the moisture retention and antioxidant activity were slightly improved, but still not as good as the effect of Example 2. The moisture retention was improved to 70%, the whitening effect (L* value) was improved to 5.0, and the antioxidant activity was 50%. The stratification phenomenon was slight. This result shows that the fission yeast fermentation extract plays a significant role in moisture retention and antioxidant, and the lack of this ingredient will lead to a decrease in the overall effect.

[0251] Comparison with a formulation lacking fermented white truffle extract (Test Experiment C):

[0252] Test experiment C did not contain fermented white truffle extract, but contained fission yeast fermented extract. The moisturizing, whitening and anti-oxidation effects were better than those of test experiments A and B, but lower than those of Example 2. The moisturizing degree was 72%, the whitening effect (L* value) was 5.5, and the antioxidant activity was 55%. The stratification phenomenon was slight.

[0253] This result shows that fermented white truffle extract makes important contributions in anti-oxidation and whitening, and its synergistic effect with fission yeast fermentation extract can significantly enhance the skin care effect.

[0254] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An antioxidant whitening and repairing ingredient based on fission yeast fermentation technology, characterized in that: Includes the following mass percentages: 5%-15% fission yeast fermentation extract; 2%-6% niacinamide; 1%-4% ethyl ascorbic acid; 0.5%-2% ceramide complex; 0.5%-1.5% sodium hyaluronate; 0.1%-0.5% beta-glucan; 0.1%-0.5% hexapeptide; 1%-5% glycerol; 40%-80% water; 0.5%-2% fermented white truffle extract; 0.5%-1.5% sea fennel stem cell extract; 0.5%-1.5% phospholipids; 0.5%-2.5% plant squalane; 0.1%-0.5% chamomile extract; 0.01%-0.1% lavender essential oil.

2. The antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 1, characterized in that: The preparation steps of the fission yeast fermentation extract are as follows: Inoculate the fission yeast strain into the culture medium; Cultivate in a fermenter, adjust the initial pH value of the culture medium to -6.0-7.0, set the temperature to 35°C-40°C, introduce oxygen, the oxygen flow rate is 0.5vvm-1.5vvm, the fermentation time is 24H-72H, monitor the OD600 value of the fermentation liquid during this period, and keep the bacterial concentration at 1OD-5OD; After the fermentation is completed, the fermentation liquid is centrifuged at a speed of 2000 rpm to 6000 rpm and at 0°C to 10°C for 10 min to 30 min to obtain a supernatant; The supernatant after centrifugation is filtered through a 5kDa-15kDa ultrafiltration membrane to separate the macromolecular components, and the diafiltrate containing small molecular peptides, amino acids and antioxidant enzymes is collected; The diafiltrate is subjected to liquid-liquid extraction, and ethyl acetate having the same volume as the fermentation broth is added, and the fat-soluble components are allowed to enter the organic phase under stirring conditions, and the organic phase containing polyphenols is separated; The aqueous phase was concentrated by rotary evaporation to 1 / 5-1 / 20 of the original volume, the temperature was controlled at 30°C-40°C, and then vacuum freeze-dried at -10°C--50°C to obtain the fission yeast fermentation extract.

3. A method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology, preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to any one of claims 1-2, characterized in that: The following steps are involved: S1. Inoculating the fission yeast strain into a culture medium containing glucose, peptone and yeast extract, and obtaining a fission yeast fermentation extract after culturing, centrifugation, ultrafiltration and drying; S2, inoculating white truffle strains into a culture medium containing glucose, malt extract and amino acids, and obtaining a fermented white truffle extract by fermentation, ultrafiltration and drying; S3, preparing an aqueous phase: adding fission yeast fermentation extract, niacinamide, ethyl ascorbic acid, ceramide complex, sodium hyaluronate, β-glucan, hexapeptide and glycerol into water and dissolving them to obtain an aqueous phase; S4, preparing an oil phase: mixing plant squalane, phospholipids, fermented white truffle extract, sea fennel stem cell extract, chamomile extract and lavender essential oil to obtain an oil phase; S5. Slowly add the oil phase into the water phase and prepare a uniform emulsion after emulsification.

4. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 3, characterized in that: The emulsification step in step S5 is: S51, stirring at a speed of 3000 rpm-8000 rpm for 5 min-10 min; S52, use 20kHz-40kHz ultrasonic treatment for 10min-20min.

5. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 3, characterized in that: The culture medium in step S1 contains 5g / L-30g / L glucose, 5g / L-15g / L peptone and 3g / L-10g / L yeast extract, the pH value is adjusted within the range of 6.0-7.0, and the culture is carried out at 35°C-40°C for 24H-72H.

6. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 3, characterized in that: The preparation of the fermented white truffle extract in step S2 comprises the following steps: S21, inoculating the white truffle strain into a culture medium containing 5g / L-20g / L glucose, 3g / L-10g / L malt extract and 0.5g / L-5g / L amino acids, controlling the temperature at 25°C-30°C, and fermenting for 48H-96H; S22, after the fermentation is completed, filtering through a 5kDa-15kDa ultrafiltration membrane to obtain a filtrate; S23. Freeze-drying the filtrate to obtain a fermented white truffle extract.

7. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 3, characterized in that: In step S3, the aqueous phase material is stirred at room temperature at a speed of 500 rpm-1500 rpm for 15 min-30 min.

8. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 3, characterized in that: In the step S4, the temperature of the oil phase materials during mixing is maintained at 40° C.-60° C.

9. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 3, characterized in that: The mass ratio of the water phase to the oil phase is 1:1-1:

4.

10. The method for preparing an antioxidant whitening and repairing ingredient based on fission yeast fermentation technology according to claim 6, characterized in that: The S23 freeze-drying temperature is -10°C to 50°C.

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