Application of effective component formula of Guiaojiaogangrene in treatment of cardiovascular and cerebrovascular diseases caused by diabetes mellitus

Abstract

The invention relates to the technical field of medicines, in particular to application of an effective component formula of Guiaojiaogangrene in treatment of cardiovascular and cerebrovascular diseases caused by diabetes mellitus. The effective component formula of the Guiaosugangrene comprises the following components: n-butyl lactone, esculetin, pyroxylic acid, monolatreoside, lonicicin A, hypochaulinin, coptisaponin II, palmatine, taxifolin and angoroside B. The invention further discloses a preparation method of the Guiaosugangrene composition, and the preparation method of the Guiaosugangrene composition comprises the following steps: preparing the Guiaosugangrene composition. The medicine prepared according to the formula has an excellent treatment effect on cardiovascular and cerebrovascular diseases caused by diabetes.

Description

Technical Field

[0001] The present invention relates to the field of pharmaceutical technology, and more specifically to the application of a formula of effective ingredients of Gastrodia elata in treating cardiovascular and cerebrovascular diseases caused by diabetes. Background Art

[0002] Cardiovascular and cerebrovascular diseases are extremely common diseases in my country's medical treatment. They usually refer to common diseases related to the heart and brain blood vessels, mainly including coronary heart disease, cerebrovascular disease, hypertension, heart disease, etc. Cardiovascular and cerebrovascular diseases are characterized by high morbidity and extremely high mortality. Every year, more than 15 million people die from this disease worldwide, and the mortality rate is rising, seriously threatening people's health, especially the elderly. Its cause and mechanism are still unclear, and most researchers tend to believe that chronic damage to organs is caused by multiple other factors such as diabetes, hypertension, and obesity.

[0003] At present, most clinical treatments for cardiovascular and cerebrovascular diseases include antihypertensive drugs, hypoglycemic drugs and other indicators that can control symptoms, but there are risks of not being able to stop taking the drugs, as well as many adverse reactions. Traditional Chinese medicine has the advantages of multi-target, multi-component and holistic intervention for cardiovascular and cerebrovascular diseases. Zhang Xichun, a modern Chinese medicine expert, proposed that "the spirit of man is based on the heart and brain, and the function of the spirit is based on the mutual assistance of the heart and brain". According to the theory of traditional Chinese medicine, the etiology of cardiovascular and cerebrovascular diseases is "the root is deficiency and the symptoms are excess", the root is deficiency of heart and kidney yang, and the symptoms are phlegm turbidity and blood stasis. The main drug treatment is blood-activating and blood-stasis-removing drugs. Guizhi Tangju Granules are composed of nine Chinese medicines: Angelica sinensis, Anemarrhena asphodeloides, Honeysuckle, Coptis chinensis, Rehmannia glutinosa, Scrophularia ningpoensis, Licorice, and Polygonum multiflorum. Among them, Angelica sinensis and Polygonum multiflorum are mainly used to activate blood circulation and dissipate blood stasis, while Anemarrhena asphodeloides, Honeysuckle, Coptis chinensis, Rehmannia glutinosa, Scrophularia ningpoensis, Licorice and other medicines are mainly used to clear away heat and detoxify, soothe wind and dredge collaterals. In general, there is no relevant research on the application of the formula of effective ingredients of Guizhi Sugarcane in the preparation of treatment for cardiovascular and cerebrovascular diseases caused by diabetes. Summary of the invention

[0004] In order to make up for the deficiencies of the prior art, the present invention provides an application of a formula of effective ingredients of Radix Angelicae Sinensis in treating cardiovascular and cerebrovascular diseases caused by diabetes.

[0005] The present invention is achieved through the following technical solutions:

[0006] In the first aspect, the present invention provides an application of an effective ingredient formula of Guizhi sugar carbuncle in the treatment of cardiovascular and cerebrovascular diseases caused by diabetes, wherein the effective ingredient formula of Guizhi sugar carbuncle comprises the following ingredients: n-butyl lactone, esculin, lignoceric acid, monomelite glycoside, honeysuckle glycoside A, cypermethrin, berberine saponin II, palmatine, taxifolin, and angoroside B.

[0007] Preferably, the mass ratio of each component of the effective ingredient formula of the Guizhi carbuncle is: n-butyl lactone: esculent acid: lignoceric acid: monomelite glycoside: honeysuckle glycoside: cyperus rotundus: coptis saponin II: palmatine: taxifolin: angoside B: (3.8-4.6): (1.3-1.8): (0.9-1.3): (1.2-1.7): (1.0-1.6): (0.5-0.9): (2.5-3.4): (2.8-3.6): (2.1-2.8): (0.3-0.8).

[0008] Preferably, the preferred mass ratio of each component of the effective ingredient formula of Guizhi Sugar Carbuncle is: n-butyl lactone: esculent acid: lignoceric acid: monomelite glycoside: honeysuckle glycoside: cyperus rotundus: coptis saponin II: palmatine: taxifolin: angoside B is: 4.1:1.5:1.1:1.4:1.3:0.7:2.9:3.2:2.6:0.7.

[0009] Preferably, the formula of the effective ingredients of Glehnia littoralis can enhance the free radical scavenging activity, reduce the damage of free radicals to myocardial tissue, and have a better protective effect on myocardial tissue.

[0010] Preferably, the formula of the effective ingredients of Guizhi sugar cane can reduce the abnormal elevation stage of the ST segment, decrease the SPK level, protect the SOD activity of the myocardial tissue, and have a better protective effect on the myocardial tissue.

[0011] In a second aspect, the present invention provides a drug for treating cardiovascular and cerebrovascular diseases, the drug comprising a formula of effective ingredients of Gastrodia elata, including the following ingredients: n-butyl lactone, esculent acid, lignoceramide, monomelitrin, honeysuckle glycoside A, pyrrolidone, berberine saponin II, palmatine, taxifolin, and angoside B.

[0012] Compared with the prior art, the present invention is beneficial in that: the present invention provides a formula of effective ingredients for treating cardiovascular and cerebrovascular diseases caused by diabetes, and a drug prepared using the formula, wherein the drug has excellent therapeutic effects on cardiovascular and cerebrovascular diseases caused by diabetes. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present invention will be further described below in conjunction with the accompanying drawings.

[0014] Figure 1 Effects on the ST segment of the electrocardiogram in rats with acute myocardial ischemia;

[0015] Figure 2 Effects on serum MDA in rats with acute myocardial ischemia;

[0016] Figure 3 Effects on serum CPK in rats with acute myocardial ischemia;

[0017] Figure 4 Effects on serum SOD in rats with acute myocardial ischemia. DETAILED DESCRIPTION

[0018] The present invention is described in further detail below so that those skilled in the art can implement it according to the description.

[0019] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial sources unless otherwise specified. In addition, the pharmaceutical preparation used in the present invention is a granule.

[0020] Example 1: Formulation of the active ingredient of Gyuzhi saccharomyces

[0021] The mass ratios of the various components in the formula of the effective ingredient of Guizhi Cannabis Sativa are as follows: n-butyl lactone: esculent acid: lignoceramide: monomelitrin: honeysuckle glycoside: cyperus rotundus: coptis saponin II: palmatine: taxifolin: angoside B: (3.8-4.6): (1.3-1.8): (0.9-1.3): (1.2-1.7): (1.0-1.6): (0.5-0.9): (2.5-3.4): (2.8-3.6): (2.1-2.8): (0.3-0.8).

[0022] In this embodiment, the mass ratio of the effective ingredient formula of Guizhi Cannabis Sativa is 4.1:1.5:1.1:1.4:1.3:0.7:2.9:3.2:2.6:0.7.

[0023] Example 2: Experiment on the treatment of acute myocardial ischemia caused by diabetes mellitus in rats with Guizhi Tangwu granules

[0024] 1. Materials

[0025] 1.1 Experimental animals

[0026] 60 male SPF Wistar rats weighing 150-200 g were provided by Chengdu Dashuo Experimental Animal Co., Ltd. All experimental animals were kept in a controlled environment with a room temperature of 20-25°C and a humidity of 40%-50%. During the experiment, the animals had free access to food and water, and their circadian rhythms were normal.

[0027] 1.2 Main drugs and reagents

[0028] Guizhi Sugar Antler Granules (prepared into granules according to the mass ratio of Example 1); diltiazem hydrochloride: 0.3 mg / kg,

[0029] Purchased from Tianjin Tanabe Pharmaceutical Co., Ltd.

[0030] 2. Experimental process

[0031] 2.1 Modeling and grouping

[0032] After two weeks of adaptive feeding, 60 male Wistar rats were randomly selected from the normal group and the remaining 45 rats were used to model diabetic cardiovascular and cerebrovascular diseases. They were divided into a model group, a Chinese medicine group, and a Western medicine group, with 15 rats in each group. The model was established by subcutaneous injection of 30 mg / kg of isoproterenol to induce a rat myocardial ischemia model. Electrocardiograms were recorded in each group before administration. After 6 hours of administration of isoproterenol or saline, electrocardiograms were repeated, and then blood was collected from the femoral artery and the heart was removed to prepare homogenate.

[0033] 2.2 Administration

[0034] Drugs were administered 2 hours after modeling.

[0035] The model group and the normal group were intragastrically administered with normal saline (30 mg / kg).

[0036] The Chinese medicine group was given Guizhi Tangju Granules aqueous solution (concentration 30 mg / kg) by intragastric administration.

[0037] Western medicine group: Diltiazem hydrochloride was added to distilled water to prepare a 20ug / ml suspension for oral administration.

[0038] 2.3 Electrocardiogram measurement

[0039] ST segment deviation is an indicator of myocardial ischemia and drug efficacy. In this experiment, normal lead II electrocardiograms were recorded for each group of animals, and the total millivolt value of ST segment deviation after administration of isoproterenol was measured. The number of animals with abnormal ST segment deviation was recorded as positive and the deviation mean.

[0040] 2.4 Preparation of myocardial homogenate and determination of serum creatine phosphokinase (CPK), superoxide dismutase (SOD), and lipid peroxide malondialdehyde (MDA)

[0041] After the heart was removed, residual blood was removed, the heart was weighed, placed in physiological saline below 0°C, and ground at 4°C to make a 20% homogenate. CPK was determined using an automatic biochemical analyzer; myocardial homogenate SOD was determined using a modified pyrogallol auto-oxidation method. MDA was determined using a spectrophotometer.

[0042] 3. Results

[0043] 3.1 Effects on the ST segment of the electrocardiogram in rats with acute myocardial ischemia

[0044] The results of the effects of the drug of the present invention on the ST segment of the electrocardiogram of rats with acute myocardial ischemia are shown in Table 1 below. Compared with the normal group, the ST segment deviation of the model group was significantly increased. Compared with the model group, the ST segment deviation of the Chinese medicine group and the Western medicine group was significantly reduced.

[0045] Table 1 Effects on ST segment of electrocardiogram in rats with acute myocardial ischemia (n=15)

[0046]

[0047] Note: *Compared with the normal group (p < 0.05); #Compared with the model group (p < 0.05)

[0048] 3.2 Effects on serum MDA, CPK and SOD in rats with acute myocardial ischemia

[0049] Table 2 Effects on MDA, CPK and SOD in rats with acute myocardial ischemia (n=15)

[0050]

[0051] Note: *Compared with the normal group (p < 0.05); #Compared with the model group (p < 0.05)

[0052] The effects of the drug of the present invention on serum MDA, CPK and SOD in rats with acute myocardial ischemia are shown in Table 2 above. Compared with the normal group, the model group had significantly increased myocardial tissue c(MDA) / nmol*L-1 concentration and CPK concentration, and significantly decreased SOD concentration. Compared with the model group, the myocardial tissue c(MDA) / nmol*L-1 concentration and CPK concentration in the Chinese medicine group and the Western medicine group had significantly decreased SOD concentration.

[0053] The present invention uses isoproterenol to induce acute myocardial ischemia in rats, and the abnormal ST segment of the electrocardiogram increases, the serum CPK activity increases, the MDA concentration increases, and the free radical scavenger SOD activity decreases. The Guizhi sugar ulcer granules of the present invention (prepared according to the mass ratio of embodiment 1) can reduce the abnormal ST segment elevation stage, reduce the SPK level, and have a good protective effect on myocardial tissue. In addition, this medicine can protect the SOD activity of myocardial tissue, enhance the free radical scavenging activity, and can reduce the damage of free radicals to myocardial tissue.

Claims

1. An application of a formula of effective ingredients of Gyuzhi saccharum officinale in treating cardiovascular and cerebrovascular diseases caused by diabetes, characterized in that: The effective ingredient formula of the Guizhi saccharomyces cerevisiae comprises the following components: n-Butyl lactone, esculent acid, lignoceramide, monomelite glycoside, honeysuckle glycoside A, cypermethrin, berberine saponin II, palmatine, taxifolin, and angoside B.

2. The use according to claim 1, characterized in that: The mass ratios of the various components of the effective ingredient formula of the Guizhi carbuncle are: n-butyl lactone: esculent acid: lignoceric acid: monomelite glycoside: honeysuckle glycoside: cyperus rotundus: coptis saponin II: palmatine: taxifolin: angoside B: (3.8-4.6): (1.3-1.8): (0.9-1.3): (1.2-1.7): (1.0-1.6): (0.5-0.9): (2.5-3.4): (2.8-3.6): (2.1-2.8): (0.3-0.8).

3. The use according to claim 2, characterized in that: The preferred mass ratio of each component of the effective ingredient formula of Guizhi Sugar Carbuncle is: n-butyl lactone: esculent acid: lignoceric acid: monomelite glycoside: honeysuckle glycoside: cyperus rotundus: coptis saponin II: palmatine: taxifolin: angoside B is: 4.1:1.5:1.1:1.4:1.3:0.7:2.9:3.2:2.6:0.7。 4. The use according to claim 1, characterized in that: The formula of the effective ingredients of Glehnia littoralis can enhance the free radical scavenging activity, reduce the damage of free radicals to myocardial tissue, and have a good protective effect on myocardial tissue.

5. The use according to claim 1, characterized in that: The formula of Guizhi sugar cane effective ingredients can reduce the abnormal elevation stage of ST segment, decrease SPK level, protect the SOD activity of myocardial tissue, and have a good protective effect on myocardial tissue.

6. A drug for treating cardiovascular and cerebrovascular diseases, characterized in that: The medicine comprises a formula of effective ingredients of saccharomyces cerevisiae, which comprises the following ingredients: n-butyl lactone, esculent acid, lignoceramide, monomelite glycoside, honeysuckle glycoside A, cypermethrin, coptis saponin II, palmatine, taxifolin, and angoside B.

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