High-precision automatic liquid dilution workstation and use method thereof

Abstract

The invention relates to a high-precision automatic liquid dilution workstation and a use method thereof. The workstation comprises a rack, and the rack is provided with a manual pipette assembly, an automatic pipette assembly, a waste suction head collection bin, a suction head storage bin, a diluent storage bottle, a liquid dilution operation table and a holder camera; the manual pipette assembly and the automatic pipette assembly are used for respectively transferring a diluent, a stock solution and a mixed solution, accurate positioning operation is realized through a multi-axis linear motion mechanism, and the automatic pipette assembly is provided with a suction head removing structure; the liquid dilution operation table comprises a Y-axis linear motion mechanism, a supporting platform, a miniature magnetic stirrer and a penicillin bottle; the application method comprises the following steps of: sequentially distributing a diluent, transferring a stock solution and a mixed solution, and stirring and mixing the stock solution and the mixed solution. The device is simple and reliable in structure, high in high-precision part interchangeability, high in dilution precision, high in automation degree and few in personal error, has the advantage of multifunctional integration, and is wide in application prospect.

Description

Technical Field

[0001] The present application relates to the technical field of liquid dilution, and in particular to a high-precision automated liquid dilution workstation and a method for using the same. Background Art

[0002] In fields such as chemistry and biology, experimenters often need to dilute high-concentration solutions to obtain low-concentration target solutions for subsequent experiments. In order to prevent errors caused by a one-time large-scale dilution, continuous small-scale dilutions are generally used to obtain low-concentration target solutions. In addition, in application scenarios that require batch processing, such as medical testing, microbiological testing, and environmental inspections, manual and long-term repeated dilution operations will inevitably lead to various human errors.

[0003] At present, most experimenters still use pipettes (manual or electric) to manually complete highly repetitive liquid dilution work; only a few laboratories are equipped with automated dilution equipment. The reason is that the complex integrated structure design and the large number of high-precision parts with low interchangeability have led to high prices, which hinder the popularization of automated dilution equipment. Therefore, it is very necessary to design a dilution workstation with a simple and reliable structure and high interchangeability of high-precision parts. Summary of the invention

[0004] The present invention is mainly aimed at the above-mentioned problems existing in the prior art, and provides a high-precision automated liquid dilution workstation and a use method thereof.

[0005] The purpose of the present invention is mainly achieved through the following solutions: A high-precision automated liquid dilution workstation comprises a frame, on which a manual pipette gun assembly, an automatic pipette gun assembly, a waste tip collection bin, a tip storage bin, a diluent storage bottle, a liquid dilution operation table and a pan-tilt camera are arranged; the manual pipette gun assembly is used to complete the automated transfer of the diluent, comprises a first X-axis linear motion mechanism, a first Z-axis linear motion mechanism, a third Z-axis linear motion mechanism, a first column and a manual pipette gun, two vertically arranged first columns are symmetrically arranged on the left and right sides of the upper surface of the frame, the first X-axis linear motion mechanism is installed between the two first columns, the first Z-axis linear motion mechanism is connected to the first X-axis linear motion mechanism, and the first Z-axis linear motion mechanism can drive the first X-axis linear motion mechanism to move the first Z-axis linear motion mechanism to move ... The motion mechanism moves up and down along the first column, the third Z-axis linear motion mechanism is installed on the first X-axis linear motion mechanism, and the first X-axis linear motion mechanism can drive the third Z-axis linear motion mechanism to move left and right, the side wall of the manual pipette is connected to the third Z-axis linear motion mechanism, and the third Z-axis linear motion mechanism can press or release the upper button of the manual pipette to perform liquid suction and discharge; the automatic pipette assembly is used to complete the automated transfer of stock solution and mixed solutions of different concentrations, including a second X-axis linear motion mechanism, a second Z-axis linear motion mechanism, a second column, an automatic pipette, a stop clamp and a stop fork, two vertically arranged second columns are symmetrically arranged on the left and right sides of the upper surface of the frame, and the second X-axis linear motion mechanism is installed It is installed between the two second columns, the second Z-axis linear motion mechanism is connected to the second X-axis linear motion mechanism, and the second Z-axis linear motion mechanism can drive the second X-axis linear motion mechanism to move up and down along the second column, the automatic pipette gun is connected to the second X-axis linear motion mechanism, and the second X-axis linear motion mechanism can drive the automatic pipette gun to move left and right, a stop clamp is fixedly installed on the outer wall of the lower outer sleeve of the automatic pipette gun, and a stop fork used in conjunction with the stop clamp is fixedly installed on the side wall of one of the second columns, and the automatic pipette gun can remove the suction head through the cooperation of the stop clamp and the stop fork; the waste suction head collection bin is used to collect the waste suction heads of the automatic pipette gun, and the upper part is open and the inside is hollow, and the waste suction head collection bin is located directly above the stop fork. Below; the diluent storage bottle is used to store diluent, and is installed on the rack through a mounting bracket, and a liquid level sensor is also installed on the diluent storage bottle; the liquid dilution operating table includes a Y-axis linear motion mechanism, a support platform, a micro-magnetic stirrer and a plurality of syringe bottles, the support platform is installed on the Y-axis linear motion mechanism, and the Y-axis linear motion mechanism can drive the support platform to move forward and backward, the micro-magnetic stirrer is installed on the upper surface of the support platform, the syringe bottle is installed on the upper surface of the micro-magnetic stirrer, and a plurality of syringe bottles are arranged in sequence from left to right; the tip storage bin is installed on the upper surface of the support platform, and a plurality of tips matching the automatic pipette gun are installed on the tip storage bin; the pan-tilt camera is installed above the rack.

[0006] Preferably, the third Z-axis linear motion mechanism includes an internally hollow shell, a third Z-axis linear motion component and a pressing block. The outer wall of the shell is detachably fixed with a manual pipette via a pipette clamp, and the third Z-axis linear motion component can drive the pressing block to move up and down to press or release the upper button of the manual pipette.

[0007] Preferably, the first X-axis linear motion mechanism includes a first beam and a first X-axis linear motion assembly, both ends of the first beam are slidably connected to the first column via a first slide, the side wall of the shell is slidably connected to the first beam via a third slide, and the first X-axis linear motion assembly is connected to the third slide, and can cooperate with the third slide to drive the shell to move left and right along the first beam.

[0008] Preferably, the first Z-axis linear motion mechanism includes a first Z-axis linear motion component, a first screw rod, a second screw rod, a first driving pulley, a first driven pulley and a first synchronous belt, wherein the lower end of one of the first columns is connected to the first motor flange, the upper end of the first column is connected to the bearing seat, and the upper and lower ends of the other first column are connected to the bearing seat, the first Z-axis linear motion component is fixedly installed on the lower surface of the first motor flange, the output end of the first Z-axis linear motion component passes through the first motor flange and is fixedly connected to the lower end of the first screw rod through the first coupling, the upper end of the first screw rod is rotatably connected to the corresponding bearing seat, the upper and lower ends of the second screw rod are rotatably connected to the corresponding bearing seat, the first screw rod and the second screw rod respectively pass through the corresponding first slide table up and down, and are threadedly connected to the first slide table, the first driving pulley is fixedly sleeved on the lower end of the first screw rod, the first driven pulley is fixedly sleeved on the lower end of the second screw rod, and the first driving pulley and the first driven pulley are connected by the first synchronous belt.

[0009] Preferably, the second X-axis linear motion mechanism includes a second beam and a second X-axis linear motion assembly, both ends of the second beam are slidably connected to the second column via a second slide, the upper outer sleeve side wall of the automatic pipette gun is slidably connected to the second beam via a fourth slide, and the second X-axis linear motion assembly is connected to the fourth slide, and can cooperate with the fourth slide to drive the automatic pipette gun to move left and right along the second beam.

[0010] Preferably, the second Z-axis linear motion mechanism includes a second Z-axis linear motion assembly, a third screw rod, a fourth screw rod, a second driving pulley, a second driven pulley and a second synchronous belt, wherein the lower end of one of the second columns is connected to a second motor flange, the upper end of the second column is connected to a bearing seat, and the upper and lower ends of another second column are connected to bearing seats, the second Z-axis linear motion assembly is fixedly mounted on the lower surface of the second motor flange, the output end of the second Z-axis linear motion assembly passes through the second motor flange and is fixedly connected to the lower end of the third screw rod through a second coupling, the upper end of the third screw rod is rotatably connected to the corresponding bearing seat, the upper and lower ends of the fourth screw rod are rotatably connected to the corresponding bearing seat, the third screw rod and the fourth screw rod respectively pass through the corresponding second slide table up and down, and are threadedly connected to the second slide table, the second driving pulley is fixedly sleeved on the lower end of the third screw rod, the second driven pulley is fixedly sleeved on the lower end of the fourth screw rod, and the second driving pulley and the second driven pulley are connected through a second synchronous belt.

[0011] Preferably, the stop fork includes a fixed block and two laterally arranged baffle rods, the fixed block is fixedly mounted on the side wall of one of the first columns, and the baffle rod is mounted on the side of the fixed block facing the automatic pipette, and during the left and right movement of the automatic pipette, the stop fork will move between the two baffle rods, and during the up and down movement of the automatic pipette, the upper surface or lower surface of the stop clamp will abut against the outer wall of the baffle rod.

[0012] Preferably, the Y-axis linear motion mechanism includes a Y-axis linear motion component, a first longitudinal beam and a fifth slide, two first longitudinal beams are provided in parallel, and the fifth slide is slidably connected to the two first longitudinal beams, the upper surface of the fifth slide is connected to the lower surface of the support platform, and a connecting seat is installed on the lower surface of the support platform, the Y-axis linear motion component is connected to the connecting seat, and can cooperate with the fifth slide to drive the support platform to move forward and backward along the first longitudinal beam.

[0013] Preferably, the micro magnetic stirrer comprises a base with a hollow interior, an upper shell is provided on the upper surface of the base, a mounting seat corresponding to the vial is provided on the upper surface of the upper shell, a magnet is provided inside the base below each mounting seat, a stirring assembly is installed on the upper shell, and the magnet is driven to rotate by the stirring assembly, a magnet is provided in the vial, a variable magnetic field is formed by the rotation of the magnet, thereby driving the magnet inside the vial to rotate at high speed.

[0014] In a second aspect, the present application provides a method for using a high-precision automated liquid dilution workstation, wherein the vials are provided with four, and the method for using the vials comprises the following steps: Step S1, driving the manual pipette gun to move toward the diluent storage bottle through the first X-axis linear motion mechanism and the first Z-axis linear motion mechanism, and extending the suction head of the manual pipette gun to above the liquid surface in the diluent storage bottle, pressing the upper button of the manual pipette gun through the third Z-axis linear motion mechanism to exhaust the manual pipette gun, and then driving the manual pipette gun downward through the first Z-axis linear motion mechanism until the suction head of the manual pipette gun extends below the liquid surface of the diluent, and releasing the upper button of the manual pipette gun through the third Z-axis linear motion mechanism to absorb the diluent, and then under the linkage of the first X-axis linear motion mechanism, the first Z-axis linear motion mechanism and the Y-axis linear motion mechanism, the manual pipette gun is moved from the diluent storage bottle to the bottle mouths of the second, third and fourth syringe bottles in turn, and the upper button of the manual pipette gun is pressed and released multiple times through the third Z-axis linear motion mechanism to move the diluent into the corresponding syringe bottle; Step S2, starting a micro magnetic stirrer to drive the magnetic element inside the vial to rotate at high speed, stirring the liquid in the vial to make it evenly mixed; Step S3, driving the automatic liquid transfer gun to remove the stock solution in the first syringe bottle and inject it into the second syringe bottle through the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, completing a stock solution transfer, and then driving the automatic liquid transfer gun to automatically remove the waste tip and replace the new tip under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism; then driving the automatic liquid transfer gun to remove the mixed solution in the second syringe bottle and inject it into the third syringe bottle through the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, completing a mixed solution transfer, and then driving the automatic liquid transfer gun to automatically remove the waste tip and replace the new tip under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism; finally, driving the automatic liquid transfer gun to remove the mixed solution in the third syringe bottle and inject it into the fourth syringe bottle through the second X-axis linear motion mechanism and completing a mixed solution transfer, and then driving the automatic liquid transfer gun to automatically remove the waste tip and replace the new tip under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism.

[0015] In summary, compared with the prior art, the present invention has the following beneficial technical effects: (1) The present invention is provided with a manual pipette assembly, an automatic pipette assembly and a liquid dilution operating table. The manual pipette is matched with a linear motion mechanism to complete the automatic transfer of the dilution liquid, the electric pipette is matched with a linear motion mechanism to complete the automatic transfer of the stock liquid and mixed liquids of different concentrations, and the micro magnetic stirrer of the liquid dilution operating table completes the rapid mixing of the diluted stock liquid; the various structures are closely matched and the logic is clear, easy to maintain and operate, reducing the overall failure rate of the equipment and improving the reliability of the equipment; (2) The manual pipette and the automatic pipette in the present invention are both commercially available products, without the need for special customization. Their high interchangeability ensures that the workstation will not be shut down for a long time due to damage to a certain component, greatly reducing the maintenance cost and maintenance time of the equipment and improving the versatility and scalability of the equipment; (3) In the entire dilution process of the present invention, all operations, from the aspiration of the diluent, the transfer of the stock solution and the mixed solution to the disposal of the waste pipette tip, are completed by an automated mechanism, which reduces the manual operation links and avoids human errors caused by hand shaking and improper operation when manually operating the pipette gun, thereby improving the accuracy and repeatability of the experimental results; (4) The present invention integrates multiple functions into one. It can not only realize the dilution operation of liquid, but also is equipped with a pan-tilt camera, which can monitor and record the entire operation process, facilitating the subsequent tracing and analysis of the experimental process. At the same time, the design of the liquid dilution operation table can conveniently place multiple vials, which is suitable for batch processing of liquid dilution tasks, improves work efficiency, and meets the needs of batch liquid dilution processing in multiple fields such as medical testing, microbiological testing, and environmental protection inspection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the present invention; Figure 2 It is a front view of the present invention; Figure 3 is a top view of the present invention; Figure 4 It is a structural schematic diagram of the manual liquid transfer gun assembly of the present invention; Figure 5 It is a schematic diagram of the connection between the manual liquid transfer gun and the third Z-axis linear motion mechanism in the present invention; Figure 6 It is a schematic structural diagram of the third Z-axis linear motion mechanism in the present invention; Figure 7 It is a structural schematic diagram of the automatic liquid transfer gun assembly of the present invention; Figure 8 It is a structural schematic diagram of the driving mechanism in the present invention; Fig. 9 It is a schematic diagram of the bottom structure of the liquid dilution operating table in the present invention; Fig.10 It is a structural schematic diagram of the liquid dilution operating table in the present invention; Fig.11 It is a structural schematic diagram of the micro magnetic stirrer of the present invention; Fig.12 It is an operational process diagram of liquid dilution in the present invention.

[0017] Figure numerals: 1-frame, 2-manual pipette assembly, 3-automatic pipette assembly, 4-waste tip collection bin, 5-tip storage bin, 6-dilution storage bottle, 7-liquid dilution operation table, 8-pan-tilt camera, 9-first column, 10-manual pipette, 11-second column, 12-automatic pipette, 13-stop clamp, 14-stop fork, 15-mounting bracket, 16-liquid level sensor, 17-support platform, 18-micro magnetic stirrer, 19-syringe bottle, 20-shell, 21-third Z Axis linear motion assembly, 22-pressing block, 23-pipette fixture, 24-upper button, 25-first beam, 26-first X-axis linear motion assembly, 27-first slide, 28-third slide, 29-first Z-axis linear motion assembly, 30-first screw rod, 31-second screw rod, 32-first driving pulley, 33-first driven pulley, 34-first synchronous belt, 35-first motor flange, 36-bearing seat, 37-first coupling, 38-second beam, 39-second X-axis linear motion assembly, 40-second slide, 41-fourth slide, 42-second Z-axis linear motion assembly, 43-third screw, 44-fourth screw, 45-second driving pulley, 46-second driven pulley, 47-second synchronous belt, 48-second motor flange, 49-second coupling, 50-fixed block, 51-stop rod, 52-Y-axis linear motion assembly, 53-first longitudinal beam, 54-fifth slide, 55-connecting seat, 56-base, 57-upper shell, 58-mounting seat, 59-magnet, 60-stirring assembly, 61 -guide rod bracket, 62-motor flange, 63-screw motor, 64-moving plate, 65-slide groove, 66-guide rod, 67-guide sleeve, 68-limit switch, 69-first X-axis motor, 70-first driven roller, 71-third driving pulley, 72-third synchronous belt, 73-second X-axis motor, 74-first Y-axis motor, 75-second driven roller, 76-fourth driving pulley, 77-fourth synchronous belt, 78-stirring motor, 79-large gear, 80-small gear, 81-groove, 82-fixed rod. DETAILED DESCRIPTION

[0018] The following is a specific embodiment, and combined with the attached Figure 1-11 It should be understood that the implementation of the present invention is not limited to the following embodiments, and any form of modification and / or change made to the present invention will fall within the protection scope of the present invention.

[0019] Embodiment 1: like Figure 1 , 2As shown in Figure 3, the present invention discloses a technical solution, a high-precision automated liquid dilution workstation, comprising a frame 1, on which a manual pipette gun assembly 2, an automatic pipette gun assembly 3, a waste tip collection bin 4, a tip storage bin 5, a diluent storage bottle 6, a liquid dilution operating table 7 and a pan-tilt camera 8 are provided; wherein the manual pipette gun assembly 2 completes the automated pipetting of the diluent, the automatic pipette gun assembly 3 completes the automated pipetting of the stock solution and mixed solutions of different concentrations, the liquid dilution operating table 7 completes the rapid mixing of the diluted stock solution, the waste tip collection bin 4 is used to collect the waste tips of the automatic pipette gun assembly 3, the tip storage bin 5 is used to place new tips, the diluent storage bottle 6 is used to store the diluent, and the pan-tilt camera 8 can monitor and record the entire operation process.

[0020] The above-mentioned manual pipette assembly 2 includes a first X-axis linear motion mechanism, a first Z-axis linear motion mechanism, a third Z-axis linear motion mechanism, a first column 9 and a manual pipette 10. Two vertically arranged first columns 9 are symmetrically arranged on the left and right sides of the upper surface of the frame 1. The bottom of the first column 9 is bolted or welded to the frame 1. The first X-axis linear motion mechanism is installed between the two first columns 9. The first Z-axis linear motion mechanism is connected to the first X-axis linear motion mechanism, and the first Z-axis linear motion mechanism can drive the first X-axis linear motion mechanism to move up and down along the first column 9. The third Z-axis linear motion mechanism is installed on the first X-axis linear motion mechanism, and the first X-axis linear motion mechanism can drive the third Z-axis linear motion mechanism to move left and right. The side wall of the manual pipette 10 is connected to the third Z-axis linear motion mechanism, and the third Z-axis linear motion mechanism can press or release the upper button 24 of the manual pipette 10 to perform liquid suction and discharge. The manual pipette 10 is a commercially available product.

[0021] The automatic liquid transfer gun assembly 3 includes a second X-axis linear motion mechanism, a second Z-axis linear motion mechanism, a second column 11, an automatic liquid transfer gun 12, a stop clamp 13 and a stop fork 14. Two vertically arranged second columns 11 are symmetrically arranged on the left and right sides of the upper surface of the frame 1. The bottom of the second column 11 is bolted or welded to the frame 1. The second X-axis linear motion mechanism is installed between the two second columns 11. The second Z-axis linear motion mechanism is connected to the second X-axis linear motion mechanism, and the second Z-axis linear motion mechanism can drive The second X-axis linear motion mechanism moves up and down along the second column 11, the automatic pipette 12 is connected to the second X-axis linear motion mechanism, and the second X-axis linear motion mechanism can drive the automatic pipette 12 to move left and right, a stop clamp 13 is fixedly installed on the outer wall of the lower outer sleeve of the automatic pipette 12, and a stop fork 14 used in conjunction with the stop clamp 13 is fixedly installed on the side wall of one of the second columns 11, the automatic pipette 12 is removed from the suction head through the cooperation of the stop clamp 13 and the stop fork 14, and the automatic pipette 12 is a commercially available product.

[0022] The waste tip collection bin 4 is used to collect waste tips of the automatic pipette gun 12 , has an opening at the top and is hollow inside, and is located directly below the stop fork 14 .

[0023] The above-mentioned diluent storage bottle 6 is used to store diluent and is installed on the frame 1 through an installation bracket 15. The installation bracket 15 is welded or bolted to the frame 1, and a liquid level sensor 16 is also installed on the diluent storage bottle 6. The liquid level sensor 16 is a prior art and can be purchased on the market. When the liquid in the diluent storage bottle 6 drops to a level that the manual pipette 10 cannot be used to take the liquid, the experimenter will be reminded to add liquid.

[0024] The liquid dilution operating table 7 includes a Y-axis linear motion mechanism, a support platform 17, a micro-magnetic stirrer 18 and a plurality of syringe bottles 19. The support platform 17 is mounted on the Y-axis linear motion mechanism, and the Y-axis linear motion mechanism can drive the support platform 17 to move forward and backward. The micro-magnetic stirrer 18 is mounted on the upper surface of the support platform 17. The syringe bottles 19 are mounted on the upper surface of the micro-magnetic stirrer 18, and the plurality of syringe bottles 19 are arranged in sequence from left to right. A magnet is placed in the syringe bottle 19, and the magnet in the syringe bottle 19 is driven to rotate by the action of the micro-magnetic stirrer 18.

[0025] The tip storage bin 5 is positioned and installed on the upper surface of the support platform 17 by means of pins, and a plurality of tips matching the automatic pipette gun 12 are installed on the tip storage bin 5 .

[0026] The above-mentioned pan-tilt camera 8 is fixedly installed on the top of the frame 1 by bolts. The pan-tilt camera 8 is a prior art and can be purchased from the market.

[0027] Embodiment 2: like Figure 4 , 5 As shown, the present invention discloses another technical solution, a high-precision automated liquid dilution workstation, which is different from Example 1 in that the above-mentioned third Z-axis linear motion mechanism is composed of an internally hollow shell 20, a third Z-axis linear motion component 21 and a pressing block 22, and the outer wall of the shell 20 is detachably fixed with a manual pipette 10 through a pipette clamp 23, and the third Z-axis linear motion component 21 can drive the pressing block 22 to move up and down to press or release the upper button 24 of the manual pipette 10, thereby completing the suction and discharge of liquid.

[0028] like Figure 6As shown, the third Z-axis linear motion assembly 21 includes a guide rod bracket 61 and a motor flange 62 installed in the shell 20, the guide rod bracket 61 and the motor flange 62 are fixedly connected to the inner wall of the shell 20 by bolts or screws, and the guide rod bracket 61 is located above the motor flange 62, and a vertically arranged lead screw motor 63 is fixedly installed on the lower surface of the motor flange 62 by bolts, the lead screw portion of the lead screw motor 63 passes through the motor flange 62 and is threadedly connected to a moving plate 64, a threaded hole or nut matching the lead screw portion of the lead screw motor 63 is provided in the middle of the moving plate 64, and one side of the moving plate 64 is extended out of the shell 20 and is welded or bolted to the pressing block 22; a threaded hole or nut matching the lead screw portion of the lead screw motor 63 is provided on the outer wall of the shell 20, and ... The slide groove 65 that moves downward should be arranged vertically; at least one guide rod 66 is fixedly connected between the guide rod bracket 61 and the motor flange 62 by bolts. In this embodiment, two guide rods 66 are provided, and are respectively located at the diagonals between the guide rod bracket 61 and the motor flange 62. The guide rod 66 passes through the movable plate 64, and a guide sleeve 67 used in conjunction with the guide rod 66 is provided on the movable plate 64. The guide rod 66 is slidably connected with the guide sleeve 67. By starting the screw motor 63, the movable plate 64 and the pressing block 22 can be driven to move up and down along the length direction of the guide rod 66; a limit switch 68 is installed in the housing 20, and the limit switch 68 is located between the guide rod bracket 61 and the movable plate 64, and should be close to the position of the guide rod bracket 61.

[0029] Specifically, the first X-axis linear motion mechanism is composed of a first beam 25 and a first X-axis linear motion assembly 26. The two ends of the first beam 25 are slidably connected to the first column 9 through a first slide 27 connected by bolts. The side wall of the shell 20 is slidably connected to the first beam 25 through a third slide 28 connected by bolts. The first X-axis linear motion assembly 26 is connected to the third slide 28 and can cooperate with the third slide 28 to drive the shell 20 to move left and right along the first beam 25.

[0030] like Figure 8 As shown, the first X-axis linear motion assembly 26 is composed of a driving mechanism and a first X-axis motor 69. The first X-axis motor 69 is fixedly mounted on the rear right end of the first beam 25 by bolts. Figure 8As shown, the driving mechanism consists of four first driven rollers 70, a third driving pulley 71 and a third synchronous belt 72, wherein two first driven rollers 70 are vertically rotatably connected to the left and right ends of the first beam 25, and are connected through the third synchronous belt 72, and the third synchronous belt 72 is tensioned by the first driven rollers 70 at both ends of the first beam 25, and the rear side of the first beam 25 is located on both sides of the output end of the first X-axis motor 69, and a first driven roller 70 is arranged in parallel on both sides, which is used for guiding and auxiliary clamping of the third synchronous belt 72, and the output end of the first X-axis motor 69 is installed with a third driving pulley 71, and the third synchronous belt 72 is connected to the left and right ends of the first beam 25. The three active pulleys 71 are connected to the four first driven rollers 70 through the third synchronous belt 72. By starting the first X-axis motor 69, the four first driven rollers 70 are driven to rotate under the action of the third active pulley 71 and the third synchronous belt 72, thereby realizing the movement of the third synchronous belt 72 under the tension state, and the third slide 28 and the third synchronous belt 72 are fixedly connected by bolts, thereby realizing the left and right movement of the third slide 28 and the shell 20, but the driving mechanism is not limited to the above structure, as long as the left and right movement of the third slide 28, the shell 20 and the manual pipette gun 10 can be realized.

[0031] The above-mentioned first Z-axis linear motion mechanism is composed of a first Z-axis linear motion component 29, a first screw rod 30, a second screw rod 31, a first driving pulley 32, a first driven pulley 33 and a first synchronous belt 34, wherein the lower end of one of the first columns 9 is connected to a first motor flange 35, and the first motor flange 35 is fixedly installed on the frame 1, and the upper end of the first column 9 is connected to a bearing seat 36, and the upper and lower ends of another first column 9 are connected to bearing seats 36, and the first Z-axis linear motion component 29 adopts a first Z-axis motor, and the first Z-axis motor is fixedly installed on the lower surface of the first motor flange 35 by bolts, and the first Z-axis linear motion component 29 The output end passes through the first motor flange 35 and is fixedly connected to the lower end of the first screw rod 30 through the first coupling 37. The upper end of the first screw rod 30 is rotatably connected to the corresponding bearing seat 36. The upper and lower ends of the second screw rod 31 are rotatably connected to the corresponding bearing seats 36. The first screw rod 30 and the second screw rod 31 respectively pass through the corresponding first slide 27 up and down, and are threadedly connected to the first slide 27. The first driving pulley 32 is fixedly sleeved on the lower end of the first screw rod 30, and the first driven pulley 33 is fixedly sleeved on the lower end of the second screw rod 31, and the first driving pulley 32 and the first driven pulley 33 are connected by a first synchronous belt 34.

[0032] Embodiment 3: like Figure 1 , 7As shown, the present invention discloses another technical solution, a high-precision automated liquid dilution workstation, which is different from Example 1 in that the above-mentioned second X-axis linear motion mechanism includes a second beam 38 and a second X-axis linear motion assembly 39, and both ends of the second beam 38 are slidably connected to the second column 11 through a second slide 40, and the upper outer sleeve side wall of the automatic pipette gun 12 is slidably connected to the second beam 38 through a fourth slide 41, and the second X-axis linear motion assembly 39 is connected to the fourth slide 41, and can cooperate with the fourth slide 41 to drive the automatic pipette gun 12 to move left and right along the second beam 38.

[0033] Specifically, the second X-axis linear motion assembly 39 is composed of a driving mechanism and a second X-axis motor 73. The second X-axis motor 73 is fixed to the rear right end of the second beam 38 by bolts. The driving mechanism has the same structure and principle as the driving mechanism in the first X-axis linear motion assembly 26, which will not be repeated here.

[0034] Specifically, the second Z-axis linear motion mechanism includes a second Z-axis linear motion assembly 42, a third screw rod 43, a fourth screw rod 44, a second driving pulley 45, a second driven pulley 46, and a second synchronous belt 47, wherein the lower end of one of the second columns 11 is connected to a second motor flange 48, and the second motor flange 48 is welded or bolted to the frame 1, and the upper end of the second column 11 is connected to a bearing seat 36, and the upper and lower ends of another second column 11 are both connected to the bearing seat 36, and the second Z-axis linear motion assembly 42 adopts a second Z-axis motor, which is fixedly installed on the lower surface of the second motor flange 48 by bolts, and the second Z-axis linear motion assembly 42 The output end passes through the second motor flange 48 and is fixedly connected to the lower end of the third screw rod 43 through the second coupling 49. The upper end of the third screw rod 43 is rotatably connected to the corresponding bearing seat 36. The upper and lower ends of the fourth screw rod 44 are rotatably connected to the corresponding bearing seat 36. The third screw rod 43 and the fourth screw rod 44 respectively pass through the corresponding second slide 40 up and down, and are threadedly connected to the second slide 40. The second driving pulley 45 is fixedly sleeved on the lower end of the third screw rod 43. The second driven pulley 46 is fixedly sleeved on the lower end of the fourth screw rod 44, and the second driving pulley 45 and the second driven pulley 46 are connected by a second synchronous belt 47.

[0035] Specifically, the stop fork 14 is composed of a fixed block 50 and two laterally arranged baffle rods 51. The fixed block 50 is fixedly installed on the side wall of the second left column 11, and the baffle rod 51 is installed on the side of the fixed block 50 facing the automatic pipette 12. When the automatic pipette 12 moves left and right, it will move between the two baffle rods 51, and when the automatic pipette 12 moves up and down, the upper surface or lower surface of the stop clamp 13 will abut against the outer wall of the baffle rod 51. It should be noted here that when removing the tip, the automatic pipette 12 should first be adjusted up and down so that the stop clamp 13 is located below the stop fork 14, and then the automatic pipette 12 is moved to the left.

[0036] Embodiment 4: like Fig. 9 , 10 As shown, the present invention discloses another technical solution, a high-precision automated liquid dilution workstation, which is different from Example 1 in that the Y-axis linear motion mechanism is composed of a Y-axis linear motion component 52, a first longitudinal beam 53 and a fifth slide 54. The first longitudinal beam 53 is fixedly mounted on the frame 1, and two first longitudinal beams 53 are longitudinally arranged. The fifth slide 54 is slidably connected to the two first longitudinal beams 53. The upper surface of the fifth slide 54 is connected to the lower surface of the support platform 17 by bolts, and the lower surface of the support platform 17 is fixedly mounted with a connecting seat 55 by bolts. The Y-axis linear motion component 52 is connected to the connecting seat 55, and It can cooperate with the fifth slide 54 to drive the support platform 17 to move forward and backward along the first longitudinal beam 53; specifically, the Y-axis linear motion assembly 52 is composed of a first Y-axis motor 74, a second driven roller 75, a fourth driving pulley 76 and a fourth synchronous belt 77. The first Y-axis motor 74 is fixedly installed on the frame 1, and the fourth driving pulley 76 is fixedly sleeved on the output end of the first Y-axis motor 74. The second driven roller 75 is rotatably connected to the frame 1, and the second driven roller 75 and the fourth driving pulley 76 are connected by the fourth synchronous belt 77, and the connecting seat 55 is connected to the fourth synchronous belt 77 by bolts, thereby realizing the forward and backward movement of the support platform 17.

[0037] Specifically, Fig.11As shown, the micro magnetic stirrer 18 includes a base 56 with a hollow interior, an upper shell 57 is fixedly mounted on the upper surface of the base 56, and a plurality of mounting seats 58 corresponding to the vials 19 are mounted on the upper surface of the upper shell 57, and the bottom of the vial 19 is placed in the mounting seat 58. A magnet 59 is arranged below each mounting seat 58 in the base 56, and a stirring assembly 60 is mounted on the upper shell 57, and the magnet 59 is driven to rotate by the stirring assembly 60. A magnet is arranged in the vial 19, and a variable magnetic field is formed by the rotation of the magnet 59, thereby driving the magnet inside the vial 19 to rotate at a high speed; specifically, the stirring assembly is composed of a stirring motor 78, a large gear 79 and The base 56 is composed of a small gear 80, a stirring motor 78 is fixedly mounted on the upper shell 57, and the output end of the stirring motor 78 passes through the upper shell 57 and is fixedly connected to the large gear 79 in the inner cavity of the base 56, a fixing rod 82 is provided at the bottom of the base 56, the small gear 80 is rotatably connected to the fixing rod 82, and the upper surface of the small gear 80 is provided with two grooves 81 along the uniform circumference of its axis, the magnet 59 is embedded in the groove 81, and the small gear 80 is meshed with the large gear 79; in this embodiment, four mounting seats 58 are provided, each stirring assembly corresponds to two mounting seats 58, and each large gear 79 corresponds to two meshed small gears 80, and each small gear 80 corresponds to one mounting seat 58.

[0038] The present invention also provides a method for using a high-precision automated liquid dilution workstation, such as Fig.12 As shown, there are four syringe bottles 19, and the method of using the syringe bottle comprises the following steps: Step S1, driving the manual pipette gun 10 toward the diluent storage bottle 6 through the first X-axis linear motion mechanism and the first Z-axis linear motion mechanism, and extending the suction head of the manual pipette gun 10 above the liquid surface in the diluent storage bottle 6, pressing the upper button 24 of the manual pipette gun 10 through the third Z-axis linear motion mechanism to exhaust the manual pipette gun, and then driving the manual pipette gun 10 downward through the first Z-axis linear motion mechanism until the suction head of the manual pipette gun 10 extends below the liquid surface of the diluent, and releasing the upper button 24 of the manual pipette gun 10 through the third Z-axis linear motion mechanism to absorb the diluent, and then under the linkage of the first X-axis linear motion mechanism, the first Z-axis linear motion mechanism and the Y-axis linear motion mechanism, the manual pipette gun 10 is moved from the diluent storage bottle 6 to the bottle mouths of the second, third and fourth syringe bottles 19 in turn, and the upper button 24 of the manual pipette gun 10 is pressed and released multiple times through the third Z-axis linear motion mechanism to move the diluent into the corresponding syringe bottle 19; Step S2, by starting the micro magnetic stirrer 18, the stirring motor 78 rotates to drive the large gear 79 to rotate, the large gear 79 drives the small gear 80 to rotate, and the small gear 80 drives the embedded magnet 59 to rotate, forming a variable magnetic field, thereby driving the internal magnet of the vial 19 to rotate at a high speed, stirring the liquid in the vial 19 to make it evenly mixed; Step S3, driving the automatic liquid transfer gun 12 to transfer the stock solution in the first vial 19 and inject it into the second vial 19 through the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, completing one transfer of the stock solution, and then driving the automatic liquid transfer gun 12 to automatically remove the waste tip and replace the new tip under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism; then driving the automatic liquid transfer gun 12 to transfer the mixed solution in the second vial 19 and inject it into the third vial 19 through the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, completing one transfer of the stock solution. The mixed liquid is transferred once, and then the automatic pipette gun 12 is driven by the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism to automatically remove the waste tip and replace the new tip; finally, the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism drive the automatic pipette gun 12 to transfer the mixed liquid in the third syringe bottle 19 and inject it into the fourth syringe bottle 19, completing the transfer of the mixed liquid, and then the automatic pipette gun 12 is driven by the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism to automatically remove the waste tip and replace the new tip.

[0039] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A high-precision automated liquid dilution workstation, comprising a frame (1), characterized in that: The frame (1) is provided with a manual liquid transfer gun assembly (2), an automatic liquid transfer gun assembly (3), a waste pipette tip collection bin (4), a pipette tip storage bin (5), a diluent storage bottle (6), a liquid dilution operating table (7) and a pan / tilt camera (8); The manual liquid transfer gun assembly (2) is used to complete the automatic transfer of the diluent, and comprises a first X-axis linear motion mechanism, a first Z-axis linear motion mechanism, a third Z-axis linear motion mechanism, a first column (9) and a manual liquid transfer gun (10). Two vertically arranged first columns (9) are symmetrically arranged on the left and right sides of the upper surface of the frame (1). The first X-axis linear motion mechanism is installed between the two first columns (9). The first Z-axis linear motion mechanism is connected to the first X-axis linear motion mechanism, and the first Z-axis linear motion mechanism can drive the first X-axis linear motion mechanism to move up and down along the first column (9). The third Z-axis linear motion mechanism is installed on the first X-axis linear motion mechanism, and the first X-axis linear motion mechanism can drive the third Z-axis linear motion mechanism to move left and right along the first X-axis linear motion mechanism. The side wall of the manual liquid transfer gun (10) is connected to the third Z-axis linear motion mechanism, and the third Z-axis linear motion mechanism can press or release the upper button (24) of the manual liquid transfer gun (10) to perform liquid suction and discharge. The automatic liquid transfer gun assembly (3) is used to automatically transfer stock solutions and mixed solutions of different concentrations, and comprises a second X-axis linear motion mechanism, a second Z-axis linear motion mechanism, a second column (11), an automatic liquid transfer gun (12), a stop clamp (13) and a stop fork (14). Two second columns (11) are symmetrically arranged vertically on the left and right sides of the upper surface of the frame (1). The second X-axis linear motion mechanism is installed between the two second columns (11). The second Z-axis linear motion mechanism is connected to the second X-axis linear motion mechanism, and the second Z-axis linear motion mechanism can The automatic pipette gun (12) is connected to the second X-axis linear motion mechanism, and the second X-axis linear motion mechanism can drive the automatic pipette gun (12) to move left and right. A stop clamp (13) is fixedly installed on the outer wall of the lower outer sleeve of the automatic pipette gun (12), and a stop fork (14) used in conjunction with the stop clamp (13) is fixedly installed on the side wall of one of the second columns (11). The stop clamp (13) and the stop fork (14) are used to remove the suction head of the automatic pipette gun (12) through the cooperation of the stop clamp (13) and the stop fork (14); The waste tip collection bin (4) is used to collect waste tips from the automatic pipette gun (12), and has an opening at the top and a hollow interior, and the waste tip collection bin (4) is located directly below the stop fork (14); The diluent storage bottle (6) is used to store the diluent and is mounted on the frame (1) via a mounting bracket (15). A liquid level sensor (16) is also mounted on the diluent storage bottle (6); The liquid dilution operation table (7) comprises a Y-axis linear motion mechanism, a support platform (17), a micro-magnetic stirrer (18) and a plurality of vials (19), wherein the support platform (17) is mounted on the Y-axis linear motion mechanism, and the Y-axis linear motion mechanism can drive the support platform (17) to move forward and backward, the micro-magnetic stirrer (18) is mounted on the upper surface of the support platform (17), the vials (19) are mounted on the upper surface of the micro-magnetic stirrer (18), and the plurality of vials (19) are arranged in sequence from left to right; The pipette tip storage bin (5) is mounted on the upper surface of the support platform (17), and a plurality of pipette tips matching the automatic pipette gun (12) are mounted on the pipette tip storage bin (5); The pan / tilt camera (8) is installed above the frame (1).

2. A high-precision automated liquid dilution workstation according to claim 1, characterized in that: The third Z-axis linear motion mechanism comprises an internally hollow shell (20), a third Z-axis linear motion assembly (21) and a pressing block (22); the outer wall of the shell (20) is detachably fixed with a manual pipette (10) via a pipette clamp (23); the third Z-axis linear motion assembly (21) can drive the pressing block (22) to move up and down to press or release an upper button (24) of the manual pipette (10).

3. A high-precision automated liquid dilution workstation according to claim 2, characterized in that: The first X-axis linear motion mechanism comprises a first crossbeam (25) and a first X-axis linear motion assembly (26); both ends of the first crossbeam (25) are slidably connected to the first column (9) via a first slide (27); the side wall of the housing (20) is slidably connected to the first crossbeam (25) via a third slide (28); the first X-axis linear motion assembly (26) is connected to the third slide (28) and can cooperate with the third slide (28) to drive the housing (20) to move left and right along the first crossbeam (25).

4. A high-precision automated liquid dilution workstation according to claim 3, characterized in that: The first Z-axis linear motion mechanism comprises a first Z-axis linear motion component (29), a first screw rod (30), a second screw rod (31), a first driving pulley (32), a first driven pulley (33) and a first synchronous belt (34), wherein the lower end of one of the first columns (9) is connected to a first motor flange (35), the upper end of the first column (9) is connected to a bearing seat (36), and the upper and lower ends of another first column (9) are both connected to a bearing seat (36), the first Z-axis linear motion component (29) is fixedly mounted on the lower surface of the first motor flange (35), and the output end of the first Z-axis linear motion component (29) passes through the first motor flange (35) and is connected to the first screw rod (31). The lower end of the rod (30) is fixedly connected via a first coupling (37), the upper end of the first screw rod (30) is rotationally connected to the corresponding bearing seat (36), the upper and lower ends of the second screw rod (31) are rotationally connected to the corresponding bearing seat (36), the first screw rod (30) and the second screw rod (31) respectively pass through the corresponding first slide table (27) from top to bottom, and are threadedly connected to the first slide table (27), the first driving pulley (32) is fixedly sleeved on the lower end of the first screw rod (30), the first driven pulley (33) is fixedly sleeved on the lower end of the second screw rod (31), and the first driving pulley (32) and the first driven pulley (33) are connected via a first synchronous belt (34).

5. A high-precision automated liquid dilution workstation according to claim 1, characterized in that: The second X-axis linear motion mechanism comprises a second crossbeam (38) and a second X-axis linear motion assembly (39); both ends of the second crossbeam (38) are slidably connected to the second column (11) via a second slide (40); the side wall of the upper outer sleeve of the automatic liquid transfer gun (12) is slidably connected to the second crossbeam (38) via a fourth slide (41); the second X-axis linear motion assembly (39) is connected to the fourth slide (41) and can cooperate with the fourth slide (41) to drive the automatic liquid transfer gun (12) to move left and right along the second crossbeam (38).

6. A high-precision automated liquid dilution workstation according to claim 5, characterized in that: The second Z-axis linear motion mechanism comprises a second Z-axis linear motion component (42), a third screw rod (43), a fourth screw rod (44), a second driving pulley (45), a second driven pulley (46) and a second synchronous belt (47), wherein the lower end of one of the second columns (11) is connected to a second motor flange (48), the upper end of the second column (11) is connected to a bearing seat (36), and the upper and lower ends of another second column (11) are both connected to a bearing seat (36), the second Z-axis linear motion component (42) is fixedly mounted on the lower surface of the second motor flange (48), and the output end of the second Z-axis linear motion component (42) passes through the second motor flange (48) and is connected to the third The lower end of the screw rod (43) is fixedly connected via a second coupling (49), the upper end of the third screw rod (43) is rotationally connected to the corresponding bearing seat (36), the upper and lower ends of the fourth screw rod (44) are rotationally connected to the corresponding bearing seat (36), the third screw rod (43) and the fourth screw rod (44) respectively pass through the corresponding second slide table (40) from top to bottom, and are threadedly connected to the second slide table (40), the second driving pulley (45) is fixedly sleeved on the lower end of the third screw rod (43), the second driven pulley (46) is fixedly sleeved on the lower end of the fourth screw rod (44), and the second driving pulley (45) and the second driven pulley (46) are connected via a second synchronous belt (47).

7. A high-precision automated liquid dilution workstation according to claim 6, characterized in that: The stop fork (14) comprises a fixed block (50) and two transversely arranged stop rods (51), wherein the fixed block (50) is fixedly mounted on the side wall of one of the first columns (9), and the stop rod (51) is mounted on the side of the fixed block (50) facing the automatic liquid transfer gun (12). When the automatic liquid transfer gun (12) moves left and right, the stop rod (51) moves between the two stop rods (51), and when the automatic liquid transfer gun (12) moves up and down, the upper surface or the lower surface of the stop clamp (13) abuts against the outer wall of the stop rod (51).

8. A high-precision automated liquid dilution workstation according to claim 1, characterized in that: The Y-axis linear motion mechanism comprises a Y-axis linear motion component (52), a first longitudinal beam (53) and a fifth slide (54); two first longitudinal beams (53) are provided in parallel, and the fifth slide (54) is slidably connected to each of the two first longitudinal beams (53); the upper surface of the fifth slide (54) is connected to the lower surface of the support platform (17), and a connecting seat (55) is installed on the lower surface of the support platform (17); the Y-axis linear motion component (52) is connected to the connecting seat (55) and can cooperate with the fifth slide (54) to drive the support platform (17) to move forward and backward along the first longitudinal beam (53).

9. A high-precision automated liquid dilution workstation according to claim 8, characterized in that: The micro magnetic stirrer (18) comprises a base (56) with a hollow interior, an upper shell (57) being provided on the upper surface of the base (56), mounting seats (58) corresponding to the vials (19) being provided on the upper surface of the upper shell (57), magnets (59) being provided below each mounting seat (58) inside the base (56), a stirring assembly (60) being installed on the upper shell (57), and the magnets (59) being driven to rotate by the stirring assembly (60), and a magnet being provided inside the vial (19), and a variable magnetic field being formed by the rotation of the magnets (59), thereby driving the magnets inside the vial (19) to rotate at high speed.

10. A method for using the high-precision automated liquid dilution workstation according to any one of claims 1 to 9, characterized in that: The syringe bottle (19) is provided with four, and the method for using the syringe bottle (19) comprises the following steps: Step S1, driving the manual pipette gun (10) to move toward the diluent storage bottle (6) through the first X-axis linear motion mechanism and the first Z-axis linear motion mechanism, and inserting the tip of the manual pipette gun (10) above the liquid surface in the diluent storage bottle (6), pressing the upper button (24) of the manual pipette gun (10) through the third Z-axis linear motion mechanism to exhaust the manual pipette gun, and then driving the manual pipette gun (10) to move downward through the first Z-axis linear motion mechanism until the tip of the manual pipette gun (10) is inserted into the liquid surface of the diluent. The manual pipette (10) is moved from the diluent storage bottle (6) to the second, third, and fourth vials (19) in sequence under the linkage of the first X-axis linear motion mechanism, the first Z-axis linear motion mechanism, and the Y-axis linear motion mechanism, and the upper button (24) of the manual pipette (10) is pressed and released multiple times through the third Z-axis linear motion mechanism to move the diluent into the corresponding vials (19); Step S2, starting the micro magnetic stirrer (18) to drive the internal magnet of the vial (19) to rotate at high speed, stirring the liquid in the vial to make it evenly mixed; Step S3, driving the automatic liquid transfer gun (12) by the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism to transfer the stock solution in the first vial (19) and inject it into the second vial (19), completing one transfer of the stock solution, and then, under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, the automatic liquid transfer gun (12) automatically removes the waste tip and replaces the new tip; then, driving the automatic liquid transfer gun (12) by the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism to transfer the mixed solution in the second vial (19) and inject it into the third vial (19). , completing a mixed liquid transfer, and then, under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, the automatic liquid transfer gun (12) automatically removes the waste tip and replaces the new tip; finally, the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism drive the automatic liquid transfer gun (12) to transfer the mixed liquid in the third syringe bottle (19) and inject it into the fourth syringe bottle (19), completing a mixed liquid transfer, and then, under the drive of the second X-axis linear motion mechanism and the second Z-axis linear motion mechanism, the automatic liquid transfer gun (12) automatically removes the waste tip and replaces the new tip.

Images