Preparation method of anti-aging compound nutrient containing coenzyme and bioactive hydrogen

Abstract

The invention discloses an anti-aging compound nutrient containing coenzyme and bioactive hydrogen. The anti-aging compound nutrient contains bioactive hydrogen, coenzyme I, coenzyme II and adenosine triphosphate, the biological hydrogen element concentration of the biological active hydrogen is 1000 ppb to 1600 ppb; the total content of the coenzyme I, the coenzyme II and the adenosine triphosphate is greater than 36 ppm; the pH value of the compound nutrient is 4-6. The preparation method comprises the following steps: in the presence of a dehydrogenation catalyst ACT, carrying out biological reactions of micro-ecology, active bacteria, quasi-anaerobic and micro-fermentation on a plant extracting solution, milk, egg liquid or a herbal traditional Chinese medicine plant extracting solution to obtain the anti-aging compound nutrient containing coenzyme and bioactive hydrogen.

Description

[0001] This application is a divisional application of a patent application entitled "An anti-aging complex nutrient containing coenzymes and biologically active hydrogen, and its preparation method and application". The filing date of the original application is September 27, 2020, and the application number is CN202011029420.4. Technical Field

[0002] The invention belongs to the field of food, relates to a composite nutrient, and specifically relates to an anti-aging composite nutrient containing coenzymes and biologically active hydrogen, and a preparation method and application thereof. Background Art

[0003] Coenzyme I, also known as mitochondrial coenzyme, common abbreviations: NAD+ (oxidized form), NADH (reduced form), chemical name: nicotinamide adenine dinucleotide or nicotinamide diphosphate, English name: Nicotinamide adenine dinucleotide hydrate, molecular formula: C 21H 27N 7O 14P 2, molecular weight: 663.4.

[0004] Coenzyme I is an important coenzyme in human redox reactions and plays an important role in material metabolism and energy metabolism. At the same time, it is the only substrate of NAD+-dependent ribosyltransferase and has a series of biological functions. In our body, NAD+ is an active substance involved in thousands of important physiological reactions such as cell metabolism, redox, protein transcription, etc. It is a vital substance. It is also often called the first coenzyme of the human body.

[0005] In recent years, the foreign health industry has developed rapidly, and a variety of new and effective coenzymes and active hydrogen products have been developed to delay aging and improve immunity. At present, only a few companies in China are engaged in the production of health products related to gene therapy. The research and development of coenzymes and active hydrogen is mostly still carried out in university laboratories. Even fewer units are developing new NADH and NMN products. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a method for preparing a composite nutrient containing coenzymes and biologically active hydrogen for improving immunity, anti-oxidation and anti-aging.

[0007] In order to solve the above technical problems, the technical solution provided by the present invention is: to provide a method for preparing an anti-aging composite nutrient containing coenzymes and biologically active hydrogen, the specific steps of which include:

[0008] a) Plant extract, milk, egg liquid or herbal Chinese medicine plant extract is used as the substrate, the initial temperature of the substrate is adjusted to 38°C to 43°C, and the temperature is kept, and then the pH of the substrate is adjusted to pH=6 to 6.8, and a dehydrogenation catalyst ACT is added, and stirred for 3 to 5 minutes;

[0009] b) Leave a certain amount of space above the liquid level and then seal the reactor;

[0010] c) Standing, undergoing biochemical reactions of microecology, active bacteria, quasi-anaerobic and micro-fermentation;

[0011] d) After reacting for 2.5 to 24 hours, the sensors of the detection and control unit outside the reactor begin to detect various parameters in the reactor: temperature, pressure, interface potential ORP potential or biohydrogen concentration, pH value;

[0012] When the biohydrogen concentration reaches or exceeds 1000ppb, the reaction is terminated;

[0013] e) Keeping the reactor in a sealed state, sterilize and disinfect the composite nutrients by pasteurization;

[0014] f) Keep the reactor sealed and cool the composite nutrients to 4°C.

[0015] g) Under conditions that meet the production environment requirements for medical and health products, the complex nutrients shall be sealed in qualified containers or soft packaging bags; the products shall be refrigerated in an environment of 4°C.

[0016] In step a), after adding the dehydrogenation catalyst ACT, 1% to 5% of the total mass of the substrate is added with glucose as a carbon source.

[0017] The dehydrogenation catalyst ACT is active probiotics.

[0018] The active probiotics are lactic acid and bifidobacterium.

[0019] The pasteurization method has a temperature of 68 to 80° C. and a time of 15 to 20 minutes.

[0020] The method for preparing an anti-aging composite nutrient containing coenzymes and biologically active hydrogen also includes the following steps: concentrating the anti-aging composite nutrient solution containing coenzymes and biologically active hydrogen, and simultaneously online supplementing hydrogen active hydrogen to increase the content of reduced coenzyme I and active hydrogen, thereby obtaining an anti-aging composite nutrient concentrate containing coenzymes and biologically active hydrogen. Directly taking the anti-aging composite nutrient concentrate can ensure that the human body obtains sufficient coenzymes and is conducive to obtaining a higher absorption effect.

[0021] The method for preparing an anti-aging composite nutrient containing coenzymes and biologically active hydrogen also includes the following steps: according to the characteristics of coenzyme I, coenzyme II and ATP having different specific gravities, supercritical carbon dioxide extraction technology is used to extract the three components of coenzyme I, coenzyme II and ATP respectively, and solid powdered substances of coenzyme I, coenzyme II and ATP with a purity of 96-99wt.% are obtained respectively, which are used as raw materials for tablets, capsules or injection nutritional supplements.

[0022] The third technical problem to be solved by the present invention is to provide an application of an anti-aging composite nutrient containing coenzymes and biologically active hydrogen in food nutritional supplements, health products and cosmetics; the anti-aging composite nutrient concentrate containing coenzymes and biologically active hydrogen is used as a food nutritional supplement; the coenzyme I, coenzyme II and ATP solid powder substances with a purity of 96-99wt.% are further prepared into tablets, capsules or injections as health products and cosmetics for delaying aging and enhancing vitality.

[0023] In this application document, the substance containing biologically active hydrogen, coenzyme I, coenzyme II and adenosine triphosphate (ATP) is called an anti-aging complex nutrient, also known as "Turn Age Hydrogen Essence". "Turn Age Hydrogen Essence" is the Chinese transliteration of the abbreviation TAHE of the English word "Turn Age Hydrogen Essence".

[0024] The relationship and difference between NMN and NADH: 1) The full name of NMN is β-Nicotinamide Mononucleotide, and its Chinese name is β-nicotinamide mononucleotide. NMN enters the human body and, with the help of the enzyme NMNAT, consumes a certain amount of adenosine triphosphate (ATP) and is eventually converted into the anti-aging factor NAD+. NMN is only one of the four precursors of coenzyme I. 2) NADH is the reduced state of coenzyme I NAD+. NADH contains biologically active hydrogen. NADH enters the human body and is directly decomposed into NAD+ and hydrogen (H), while releasing a certain amount of adenosine triphosphate (ATP). Under the synergistic effect of the three, it not only has a comprehensive anti-aging effect, but also performs well in improving immunity. 3) The difference between NMN and NADH: First, NMN requires the help of specific enzymes and consumes energy to synthesize NAD+; while NADH does not require the help of specific enzymes when entering the body, and can directly decompose NAD+, while also generating energy. One is synthesis and the other is decomposition. One is conditionally restricted and the other is not so restricted. Therefore, relatively speaking, the efficiency of NADH conversion to NAD+ is much higher than that of NMN. Secondly, NMN needs to consume energy. We know that energy is extremely important for life activities, while NADH is just the opposite. It can generate energy, which can be supplied to immune cells. Therefore, NADH is also very effective in improving immunity, helping the body to clear bacteria and viruses faster and strengthen the body. 4) The NADH of "Tower Nuclide" will also decompose special biologically active hydrogen, which can enter the mitochondria inside the cell, fight free radicals and inflammation from the source, and has strong antioxidant properties. Ordinary hydrogen, if in a free state, cannot enter the cell and can only remove free radicals between cells. Antioxidant properties are subject to certain restrictions.

[0025] Beneficial effects: Directly taking NADH is more efficient and effective than taking NMN. In addition to NAD+ / NADH, "Tower Nucleus" also contains three other components generated by coenzyme I: NADP+ / NADPH, ATP and biologically active hydrogen. Therefore, "Tower Nucleus" is a complex nutrient with anti-aging, anti-oxidation and direct energy increase for cells or specific organs. At the same time, the "Tower Nucleus" (weakly acidic) produced by the "Tower Nucleus" process and the special packaging and preservation process (metal cans or aluminum film soft packaging, strict sealing, low temperature 4 degrees refrigeration), and the method of taking (taking after meals or taking alkaline hydrogen-rich water at the same time) can overcome the obstacles previously encountered in taking NADH directly: fear of high temperature decomposition, fear of gastric acid decomposition and instability. As for the molecular size problem, the relative molecular weight of NADH and NMN is only twice as different, and there may be no essential difference when entering the cell membrane. The number of nutrients contained in a unit of "Tower Nucleus" is much higher than that of NMN. "Tower Nucleus" meets the food safety requirements and also meets the safety requirements for use in beauty mask cosmetics.

[0026] In addition, in the preparation process of "tower nucleus", referred to as the "tower core" process, the substrates used are mainly natural green plants or Chinese herbal medicines, animal milk, poultry eggs, etc., without chemical additives. Sometimes, scraps and discarded "waste materials" in tea, agricultural and sideline processing, and fruit processing can be used as substrates to process various types of advanced nutrient solutions, which can be used by humans, or livestock and poultry farming to improve immunity, anti-oxidation and anti-aging. It is particularly important to point out that if the substrate in the "tower core" process is selected as traditional Chinese medicine (special medicine formula, ancestral formula, secret recipe, etc.), the efficacy of the obtained Chinese medicine may be greatly improved. For example, in Example 4, the anti-new coronavirus lung clearing soup publicly recommended by my country to the world is used as a substrate, and the "tower core" process is used to obtain the "tower core" anti-new coronavirus lung clearing soup. It is shown by testing that the "tower core" anti-new coronavirus lung clearing soup has richer coenzymes and energy factors. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is the preparation flow chart of “Tower Nuclide”.

[0028] Figure 2 It is the relationship between the total content of mitochondrial proteins in "Tower Nucleus" and the substrate.

[0029] in, Figure 2 The data on the middle curve is the result of concentrating the measured data 5 times. DETAILED DESCRIPTION

[0030] Example 1

[0031] Making Herbal Hydrogen "Tower Core" Tea:

[0032] a) Prepare 7-10 grams of green tea. In order to prevent the possible presence of pesticide residues in the tea leaves, the first step is to remove the pesticide residues: Pour the prepared room temperature electrolyzed water (with a pH value of 11±0.5) into the container containing the tea leaves, submerge the tea leaves, and soak for 10-15 minutes. Take out the washed tea leaves, put them into a casserole, add 780 ml of cold water, boil it over high heat, immediately turn to low heat, keep boiling for 20 minutes, pour out the tea juice and store it in the container. Pour 785 ml of cold water into the casserole again, use the same method, keep boiling for 30 minutes, pour out the tea juice and store it in the above container with about 750 ml, a total of about 1500 ml of tea water, as the substrate for making "tower nuclide".

[0033] b) Place the substrate in the reactor. First, measure and adjust the initial temperature of the tea water to 38°C to 43°C and keep it warm. Then adjust the pH of the substrate to pH 6 to 6.8. Add 5% to 10% of the total mass of the substrate as an ACT-dehydrogenation catalyst (which can be active probiotics such as lactic acid and bifidobacteria). If necessary, add 1% to 5% of the total mass of the substrate as a carbon source as glucose. Stir for 3 to 5 minutes.

[0034] c) Make sure to leave a certain amount of space above the liquid level, and then seal the reactor tightly.

[0035] d) Let stand to start the reaction.

[0036] e) After 3 to 6 hours, the sensors of the detection and control unit outside the reactor begin to detect various parameters in the reactor: temperature, pressure, interface potential ORP potential or dissolved hydrogen concentration, pH value, etc.

[0037] When the main parameters reach the predetermined values, for example, when the hydrogen concentration reaches or exceeds 1100ppb, the reaction is terminated. The product in the reactor is the "tower core" tea. The hydrogen concentration of the "tower core" tea can reach or exceed 1600ppb.

[0038] f) Keep the reactor sealed and sterilize the "Tahe" tea using pasteurization (68-80°C, 15-20 min).

[0039] g) Keep the reactor sealed and allow the "tower core" tea to cool down to 4°C.

[0040] h) Open the reactor and seal the "Tahe" tea in a container or soft packaging bag that meets the requirements of the production environment of medical and health products (GMP workshop). Then refrigerate the product in an environment of 4℃ and deliver it to the market or consumers using cold chain logistics.

[0041] Example 2

[0042] Make lactic acid "tart nucleus" milk.

[0043] The steps are similar to those in Example 1. The main difference is that fresh milk (goat milk, camel milk) or liquid milk prepared with milk powder or milk plus soybean powder is used.

[0044] As a substrate for making "Tower Core" milk. Steps b) to h) are basically the same. However, the time to start testing parameters in step e) is relatively short. After 2.5 to 3.5 hours, you must start paying attention, and don't be too late. Otherwise, it will over-ferment and it will be difficult to produce "Tower Core" milk. We noticed that the total amount of mitochondria contained in "Tower Core" milk is as high as 372 mg / L. It is more than twice that of the grass hydrogen "Tower Core". It shows that "Tower Core" milk has very rich anti-aging and immunity-enhancing functions. The hydrogen concentration of "Tower Core" milk can reach or exceed 1400ppb.

[0045] Example 3

[0046] Make 4 kinds of Herba Immortalis "Tower Core" health essence.

[0047] The steps are similar to those in Example 1. The main difference is that 2 grams of each of the nine immortal herbs in Chinese herbal medicine, including dendrobium, licorice, American ginseng and its balancer - astragalus, for a total of 8 grams, are used. The method of boiling Chinese herbal medicine is used to add cold water and decocted twice to obtain 1.5 liters of immortal herbal active liquid, which is used as the substrate for making the immortal herbal "Tower Core" health essence. The remaining steps b) to h) are almost the same. We noticed that the total amount of mitochondria contained in the "Tower Core" made with 3 to 4 immortal herbs is as high as 410.3 mg / L. It is more than twice that of "Tower Core" tea and higher than "Tower Core" milk. It is suitable for middle-aged and elderly people to supplement the need for a large amount of coenzymes in the body due to aging. The hydrogen concentration of the immortal herbal "Tower Core" health essence can reach or exceed 1300ppb.

[0048] Example 4

[0049] Make the "Tahe" lung-clearing soup to fight the new coronavirus.

[0050] The steps are similar to those in Example 3. The main difference is that the active liquid decocted from the 20 herbs in the Qingfei secret recipe is used as the substrate for making the "Tahe" Qingfei soup. The components of these 20 Chinese herbal medicines are: honeysuckle, platycodon, agarwood, patchouli, tangerine peel, shishi, yam, winter flower, aster, ginger, astragalus, bupleurum, tuckahoe, atractylodes, umbellate, oriental water plantain, cassia twig, gypsum, liquorice, and ephedra. The weight of each component is determined by the optimized formula of the secret recipe.

[0051] The production steps b) to h) are almost identical. We noticed that the total amount of mitochondria contained in the "Tower Nucleus" made from 20 Chinese herbal medicines is as high as 857.9 mg / L. This is the "Tower Nucleus" with the highest total amount of mitochondria obtained in the experiment of the present invention. The hydrogen concentration of the "Tower Nucleus" Qingfei Decoction can reach or exceed 1100 ppb.

[0052] Example 5

[0053] Make plant "Ta Nucleus" beauty essence.

[0054] The steps are similar to those in Example 1. The main difference is that the active liquid of several plant components is used: fungus (10-15 grams), white fungus (10-15 grams), fresh taro (5-10 grams), yam (5-10 grams), skimmed milk powder (3-5 grams), egg white powder (3-5 grams), tower core tea (1500-2000 milliliters), and drinking water (2500-3000 milliliters).

[0055] The production steps b) to h) are almost identical. The measured results of the "Tower Nucleus" beauty essence are as follows: active hydrogen concentration C = 1157ppb; pH = 6.1; NAD+ = 1.2117mg / L; NADH = 4.4902mg / L; NADP+ = 0.5549mg / L; NADPH = 3.6942mg / L; ATP = 124.4157mg / L; converted to the total effective mitochondrial content of 135.47ppm. This product is composed of plant food, without chemical additives, rich in anti-aging complex nutrients and active hydrogen, and has the functions of anti-oxidation, skin cell nutrition, and anti-wrinkle. It can be used to make facial masks or applied to the skin and face for skin care.

[0056] Attached Figure 1 Analysis Notes:

[0057] 1) Figure 1 The present invention is a preliminary result of 52 test samples prepared by the preparation method disclosed in the present application, and the coenzyme content (including coenzyme I, coenzyme II, ATP) of the samples was tested and the data were analyzed and collated;

[0058] 2) Since coenzyme II and ATP are products generated with coenzyme I as substrate, in order to measure and compare the quality of "mitochondria" produced by different substrates, we converted the corresponding relationship between the molar amounts of coenzyme II and ATP through biochemical reactions into equivalent (or equivalent) coenzyme I (i.e., mitochondria), and then added them to the measured mitochondria content to obtain the total mitochondria content as a basis for comparison.

[0059] 3) From Figure 2The following preliminary conclusions can be drawn: (a) The mitochondrial cytokines content obtained with oxalocyanate as a substrate is not as high as that obtained with lactocyanate and ovale as substrates; (b) The mitochondrial cytokines content obtained with green tea as a substrate is higher than that obtained with snow pear and pitaya as substrates; (c) The mitochondrial cytokines content obtained with ovale (protein albumen) as a substrate is higher than that of oxalocyanate and lactocyanate. (d) The mitochondrial cytokines content produced by using multiple (more than 3) oxalocyanate-containing plants (oxalocyanate) as substrates is much higher than that of single oxalocyanate. This may be due to the synergistic effect of different functional herbs, which greatly improves the efficiency of producing mitochondrial cytokines content. The Anti-COVID-19 Qingfei Decoction (containing 20 Chinese herbs) obtained amazing results, with a content of up to 857.9 mg / liter. Close to one gram of mitochondrial cytokines per liter.

[0060] Using the preparation method disclosed in this application, 52 "tower nuclei" (liquid samples) were prepared and analyzed. After testing, it was indicated that a certain concentration of NAD+ / NADH, NADP+ / NADPH and ATP components existed in each sample. (The content of biohydrogen, including grass hydrogen, lactohydrogen and egg hydrogen, reached an effective concentration of 1000ppb to 1600ppb). According to our definition of the total content of nutrients, the test results are: milk "tower nuclei" (C) = 134.06ppm; duck egg "tower nuclei" (C) = 209.31ppm; snow pear-dragon fruit "tower nuclei" (C) = 47.00ppm; edamame "tower nuclei" (C) = 44.90ppm; soybean "tower nuclei" (C) = 36.56ppm; herbal beauty "tower nuclei" (C) = 135.47ppm; new coronavirus lung clearing soup "tower nuclei" (C) = 272.21ppm. It turns out that the concentration of the original solution of "Tower Nuclide" is quite high and has complete practical value.

Claims

1. A method for preparing an anti-aging composite nutrient containing coenzymes and biologically active hydrogen, characterized in that: The following steps are involved: a) Plant extract, milk, egg liquid or herbal Chinese medicine plant extract is used as the substrate, the initial temperature of the substrate is adjusted to 38°C to 43°C, and the temperature is kept, and then the pH of the substrate is adjusted to pH=6 to 6.8, and a dehydrogenation catalyst ACT is added, and stirred for 3 to 5 minutes; b) Leave a certain amount of space above the liquid level and then seal the reactor; c) Standing, undergoing biochemical reactions of microecology, active bacteria, quasi-anaerobic and micro-fermentation; d) After reacting for 2.5 to 24 hours, the sensors of the detection and control unit outside the reactor begin to detect various parameters in the reactor: temperature, pressure, interface potential ORP potential or biohydrogen concentration, pH value; When the biohydrogen concentration reaches or exceeds 1000ppb, the reaction is terminated; e) keeping the reactor in a sealed state, sterilizing the composite nutrients by pasteurization; the pasteurization method has a temperature of 68 to 80° C. and a time of 15 to 20 minutes; f) Keep the reactor sealed and cool the composite nutrients to 4°C. g) Under conditions that meet the production environment requirements for medical and health products, the complex nutrients shall be sealed in qualified containers or soft packaging bags; the products shall be refrigerated in an environment of 4°C.

2. The preparation method according to claim 1, characterized in that: In step a), after adding the dehydrogenation catalyst ACT, 1% to 5% of the total mass of the substrate is added with glucose as a carbon source.

3. The preparation method according to claim 1, characterized in that: The dehydrogenation catalyst ACT is active probiotics.

4. The preparation method according to claim 3, characterized in that: The active probiotics are lactic acid and bifidobacterium.

5. The preparation method according to claim 1, characterized in that: The preparation steps also include: concentrating the anti-aging composite nutrient solution containing coenzymes and biologically active hydrogen, and simultaneously online supplementing hydrogen active hydrogen to increase the content of reduced coenzyme I and active hydrogen to obtain an anti-aging composite nutrient concentrate containing coenzymes and biologically active hydrogen.

6. The preparation method according to claim 1, characterized in that: The preparation steps also include: using supercritical carbon dioxide extraction technology to extract three components of coenzyme I, coenzyme II and ATP respectively, and obtaining coenzyme I, coenzyme II and ATP solid powder substances with a purity of 96-99wt.% respectively, which are used as raw materials for tablets, capsules or injection nutritional supplements.

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