Application of UCST type temperature-sensitive hydrogel loaded with bioactive substances in warming appliance

Abstract

The invention provides a UCST type temperature-sensitive hydrogel warming appliance for loading a bioactive substance. The UCST type temperature-sensitive hydrogel is prepared on the basis of 'UCST temperature-sensitive hydrogel material 2023109006712 based on gelatin and polyhydric alcohol'. According to the UCST type temperature-sensitive hydrogel loaded with the bioactive substances, hyaluronic acid mainly exists in a corium layer of skin in a colloid form at the temperature of 60 DEG C, and the hyaluronic acid mainly has a water storage function, so that the skin looks elastic. However, the hyaluronic acid gradually disappears along with the growth of age, so that the skin loses strong water storage capacity, the aging of cells is accelerated, and the problems that the skin gradually becomes dark, wrinkles and spots grow and the like are caused, so that the hyaluronic acid is crucial to the water retention and the aging resistance of the eye skin. The UCST type temperature-sensitive hydrogel warming device loaded with the bioactive substances releases water vapor containing hyaluronic acid, not only can fatigue a human body and promote blood circulation, but also has good effects on instant moisturizing and long-term moisturizing of eye skin.

Description

Technical Field

[0001] The present invention relates to the technical field of heating devices containing bioactive substances, and in particular to a process for loading bioactive substances onto heating devices. Background Art

[0002] Various heating devices are known, which contain heating materials in a breathable flat bag and use the heat generated by the heating materials to warm the body. There are many common heating devices for the human body, and common heating devices are all used on the human body.

[0003] For example, shoulder patches, by applying a heat-generating body to the human shoulder, attach the warming device to the user's joints and other movable parts, and attach it to the user's shoulder, thereby raising the temperature deep within the human skin.

[0004] In daily life, we need to use electronic products for a long time, which makes our eyes feel tired, sore and itchy. At this time, we need to take good care of them. With the progress of society, close-range eye use is inevitable. Because the ciliary muscle is in a state of contraction for a long time, the eyeball is congested, resulting in a high intraocular pressure. In addition, the extraocular muscles compress the eyeball for a long time, which hinders the normal circulation of blood in the eye. Over time, the eye axis lengthens and the refractive medium thickens, resulting in the inability of light to reach the photoreceptor cells, thus forming myopia.

[0005] The steam hot compress eye mask adopts advanced self-heating temperature control technology, which allows oxygen in the air to enter the eye mask body and then react chemically with iron powder inside the eye mask to form a heating element, releasing water vapor and heat. When using it, the human eye seems to have done a SP A, thereby relieving eye fatigue, improving eye blood circulation, relieving dry eyes caused by long-term work, study and surfing the Internet, and removing dark circles. At the same time, using it before going to bed can help users relax their minds and fall asleep as soon as possible. It is a generic product developed in response to the needs of the eye care market. It is named because it can spontaneously generate heat and steam, and is widely used.

[0006] Existing hot compress warming devices have technical and sensory defects when adding bioactive substances. For example, conventional adding methods are very likely to produce microbial risks. Even if microbial protection is done well, the added substances cannot be intuitively identified. Even if they can be identified, the transfer of active substances back and forth cannot be immediately perceived and seen with the naked eye after the product is used. Therefore, a UCST-type temperature-sensitive hydrogel warming device loaded with bioactive substances is proposed.

[0007] Hyaluronic acid is an active substance that exists naturally in organisms. It is an important component of the human cell matrix and various tissues. Studies have found that it has multiple physiological functions. It can regulate cell proliferation, migration and differentiation, has natural moisturizing function, and has the function of lubricating joints and protecting cartilage. Hyaluronic acid mainly exists in the dermis of the skin in the form of colloid. Its main function is to store water and make the skin look elastic. However, as we age, hyaluronic acid will gradually disappear, causing the skin to lose its strong water storage capacity, causing cells to age faster, resulting in the skin gradually darkening, wrinkles, spots and other problems. Therefore, hyaluronic acid is essential for the water retention and anti-aging of the eye skin.

[0008] To this end, the present invention uses hyaluronic acid as a representative of bioactive substances, conducts experimental research, adds it to UCST thermosensitive hydrogel (2023109006712 UCST thermosensitive hydrogel material based on gelatin and polyols), prepares UCST thermosensitive hydrogel loaded with hyaluronic acid, and then sprays it on the inside of the eye mask to obtain a new multifunctional high-value-added warming device. It can not only relieve eye fatigue, dryness and itching, but also has a good effect on immediate and long-term hydration of eye skin.

[0009] Therefore, there is an urgent need for a warming device loaded with bioactive substances to solve existing problems such as human fatigue and dehydration. Summary of the invention

[0010] The purpose of the present invention is to design a method for a UCST type thermosensitive hydrogel heating device for loading biologically active substances, and to achieve a phase transition of the UCST type thermosensitive hydrogel by controlling the temperature of the heating device, thereby gradually releasing the loaded biologically active substances.

[0011] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a warming device is arranged outside the heating body, ear hooks are arranged on both sides of the warming device, a load layer is arranged on the surface of the warming device, and the load layer is a UCST type thermosensitive hydrogel loaded with bioactive substances.

[0012] Furthermore, the components of the UCST-type thermosensitive hydrogel are: bioactive substances and UCST-type thermosensitive hydrogel materials.

[0013] Furthermore, the bioactive substance in the UCST-type thermosensitive hydrogel loaded with bioactive substances may be hyaluronic acid, sodium hyaluronate, polypeptide, squalane, retinol, glucosamine chondroitin, or essential oil.

[0014] Furthermore, the mass fraction of the loaded bioactive substance is 0.01%-1%.

[0015] Furthermore, the UCST type thermosensitive hydrogel material is prepared based on the patent "2023109006712 UCST thermosensitive hydrogel material based on gelatin and polyols".

[0016] Furthermore, a UCST-type thermosensitive hydrogel warming device loaded with bioactive substances comprises the following experimental steps: adding gelatin, water, polyol and bioactive substances into a clean and dry glass bottle, stirring and dissolving at 50-70° C., and cooling to obtain a UCST-type thermosensitive hydrogel loaded with bioactive substances.

[0017] Furthermore, the solvent is deionized water or ultrapure water, preferably deionized water.

[0018] Furthermore, the gelatin is acid-processed gelatin, alkaline-processed gelatin, enzyme-processed gelatin, etc.

[0019] Furthermore, the polyol is a polyol containing 2-7 hydroxyl groups in the molecular chain.

[0020] Furthermore, the mass fraction of the gelatin is 10%-50%, preferably 16.7%.

[0021] Furthermore, the dissolution temperature is 50-70°C, preferably 60°C.

[0022] Furthermore, the cooling time is 1h-12h, preferably 6h.

[0023] Furthermore, the cooling temperature is 10°C-30°C, preferably 25°C.

[0024] Furthermore, the mass fraction of the biologically active substance is 0.01%-1%.

[0025] Due to the adoption of the above technical solution, the present invention has the following advantages and beneficial effects:

[0026] The preparation process provided by the present invention is extremely easy to operate, the system is simple, the system is evenly mixed, the cost is very low, and the controlled release of bioactive substances can be achieved; compared with the industrialized padding method for adsorbing bioactive substances, the UCST-type thermosensitive hydrogel is more reliable and stable for loading bioactive substances, while avoiding the explosive release of bioactive substances, and can achieve controlled release of bioactive substances by controlling the temperature, and finally achieve better release of steam and active substances generated by the heating device during the heating process, thereby reducing human fatigue and promoting blood circulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Cross-sectional view of a heating device

[0028] Figure 2Comparison chart of phase transition temperature tested by bottle inversion method

[0029] In the figure: 1. heating device; 2. heating element; 3. ear hook; 4. load-bearing layer. DETAILED DESCRIPTION

[0030] In order to explain the present invention more clearly, the present invention is further described below in conjunction with preferred embodiments. It should be understood by those skilled in the art that the content described below is illustrative rather than restrictive, and should not be used to limit the scope of protection of the present invention.

[0031] The heating device 1 is a heating device in which a heating part containing an easily oxidizable metal, a reaction accelerator, an electrolyte and water is contained in a containing body, and the heating device is a heating device of a UCST type thermosensitive hydrogel loaded with a bioactive substance.

[0032] The warming device 1 can be a shoulder patch, a neck patch, a nipple patch, a foot patch, a knee patch, a waist patch, or a steam eye mask.

[0033] Among them, a heating device containing a UCST type thermosensitive hydrogel loaded with bioactive substances is added to a mixture containing an easily oxidizable metal, a reaction accelerator, an electrolyte and water, thereby obtaining a heating device.

[0034] Regarding the easily oxidizable metal, as long as it is a metal that generates heat through oxidation, there is no particular limitation. For example, powders or fibers such as iron, aluminum, zinc, manganese, magnesium, and calcium can be cited. Among these substances, iron powder is preferably used from the viewpoints of operability, safety, and manufacturing cost. In the case where the easily oxidizable metal is a powder, its particle size is preferably 10 to 300um. It is particularly preferred that the content of easily oxidizable metals with a particle size of 10 to 150um in all easily oxidizable metals is 50% by mass. Relative to the total mass of the heating device, the content of easily oxidizable metals is preferably 20 to 80% by mass or more. Relative to the total mass of the heating device, the content of easily oxidizable metals is preferably 20 to 80% by mass, more preferably 30 to 70% by mass, and particularly preferably 45 to 65% by mass. In these cases, sufficient calorific value can be ensured and therefore preferred.

[0035] Regarding the reaction accelerator, the so-called "reaction accelerator" refers to a substance having the function of an agent for retaining oxygen / supplying oxygen to the easily oxidized metal. As the reaction accelerator, a substance that functions as a moisture retaining agent is particularly preferred. Examples thereof include activated carbon (coconut shell charcoal, charcoal powder, asphalt charcoal, peat, lignite), carbon black, acetylene black, graphite, zeolite, perlite, vermiculite, silica, etc. Among these substances, activated carbon is preferred from the viewpoint of having moisture retaining performance, oxygen supply performance, and catalyst performance.

[0036] Water is preferably used in an amount of 10 to 50% by mass, preferably 15 to 40% by mass, more preferably 20 to 35% by mass, and particularly preferably 3 to 10% by mass, relative to the total mass of the heating device, because its reaction-promoting effect can be fully exerted in these cases.

[0037] The water is contained in a ratio of 10 to 50 mass %, preferably 15 to 40 mass %, and more preferably 20 to 35 mass % relative to the total mass of the heating device. The reason is that when the amount is within this range, the oxidation reaction of the oxidizable metal can be well promoted and sufficient water vapor can be generated.

[0038] Regarding electrolytes, the so-called "electrolyte" refers to a substance that dissolves in water to form an electrolytic solution. Particularly preferred are substances that further promote the oxidation reaction of easily oxidized metals. As electrolytes, for example, sodium chloride, potassium chloride, calcium chloride, magnesium chloride, etc. can be cited. Among them, from the viewpoints of operability, safety, manufacturing cost, etc., sodium chloride is particularly preferred. Relative to the total mass of the warming device, the content of the electrolyte is preferably 0.1 to 15 mass%, more preferably 0.5 to 14 mass%, and particularly preferably 1 to 12 mass%. If the electrolyte is included in these proportions, the heating temperature can be well maintained.

[0039] UCST type thermosensitive hydrogel heating device loaded with bioactive substances, heating device 1 is a steam eye mask,

[0040] The formula of the UCST type thermosensitive hydrogel steam eye mask loaded with bioactive substances includes 3.7g of iron powder, 0.15g of salt, 0.98g of water, 0.001g of resin, 0.002g of superoxide dismutase, 0.015g of hyaluronic acid, 0.003g of natural plant extract essence, 2.1g of activated carbon, 0.004g of transparent film and 0.003g of sleep-aiding sachet.

[0041] The natural plant extract essence consists of lavender, chamomile and rose essences, and the ratio between the lavender, chamomile and rose is 3:2:5.

[0042] The sleeping-aiding sachet is composed of basil, ginger flower and geranium essential oils, and the ratio of the basil, ginger flower and geranium is 3:5:2.

[0043] The bioactive substance in the UCST-type thermosensitive hydrogel loaded with hyaluronic acid can be high molecular weight hyaluronic acid, medium molecular weight hyaluronic acid, low molecular weight hyaluronic acid, or oligomeric hyaluronic acid.

[0044] A heating device 1 is arranged outside the heating body 2, and ear hooks 3 are arranged on both sides of the heating device 1. A load layer 4 is arranged on the surface of the heating device 1, and the load layer 4 is a UCST type thermosensitive hydrogel loaded with bioactive substances.

[0045] The components of the UCST-type thermosensitive hydrogel are: bioactive substances and UCST-type thermosensitive hydrogel materials.

[0046] The bioactive substance in the UCST-type thermosensitive hydrogel loaded with bioactive substances may be hyaluronic acid, sodium hyaluronate, polypeptide, squalane, retinol, glucosamine chondroitin, or essential oil.

[0047] The mass fraction of the loaded biologically active substance is 0.01%-1%.

[0048] The UCST type thermosensitive hydrogel material is prepared based on the patent "2023109006712 UCST thermosensitive hydrogel material based on gelatin and polyols".

[0049] In this embodiment, the phase transition temperature (°C) of the UCST-type thermosensitive hydrogel loaded with hyaluronic acid bioactive substances in this embodiment is measured by the vial inversion method, and the mass fraction (%) of the acid-processed gelatin and the mass fraction (%) of the hyaluronic acid bioactive substances are calculated by the following formula:

[0050]

[0051]

[0052] C1 represents the mass of acid-processed gelatin in the hydrogel, C2 represents the mass of deionized water in the hydrogel, C3 represents the mass of polyol in the hydrogel, and C4 represents the mass of hyaluronic acid bioactive substances in the hydrogel.

[0053] Example 1

[0054] First, adjust the oil bath to 60°C. Add a certain mass fraction of acid-based gelatin, sorbitol, hyaluronic acid bioactive substances and a certain volume of deionized water into a clean and dry glass bottle, then put the glass bottle into the preheated oil bath and dissolve under magnetic stirring. Pour the well-dissolved solution into the mold and cool it at 25°C for 6 hours to obtain the UCST-type thermosensitive hydrogel loaded with hyaluronic acid bioactive substances. Take part of the hydrogel and measure the phase transition temperature of the UCST-type hydrogel using the vial inversion method.

[0055] Table 1

[0056]

[0057] As can be seen from Table 1, the phase transition temperature of the UCST-type thermosensitive hydrogel loaded with hyaluronic acid bioactive substances was measured by the vial inversion method, and it was found that the addition of hyaluronic acid bioactive substances to the hydrogel did not have a significant effect on the phase transition temperature, indicating the feasibility of the hydrogel for loading hyaluronic acid bioactive substances.

[0058] Ultimately, loading bioactive substances through UCST-type thermosensitive hydrogels is more reliable and stable, while avoiding the explosive release of bioactive substances. The controlled release of bioactive substances can be achieved by controlling the temperature, and ultimately the steam and active substances generated by the heating device during the heating process can be better released, thereby relieving human fatigue and promoting blood circulation.

[0059] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field within the technical scope disclosed by the present invention, according to the technical scheme and inventive concept of the present invention, makes equivalent substitutions or changes, which should be covered within the protection scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0060] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

Claims

1. A UCST-type thermosensitive hydrogel heating device for loading biologically active substances, characterized in that: It comprises a heating body, a heating device is arranged outside the heating body, ear hooks are arranged on both sides of the heating device, a load layer is arranged on the surface of the heating device, and the load layer is a UCST type thermosensitive hydrogel loaded with bioactive substances.

2. The UCST-type thermosensitive hydrogel loaded with biologically active substances according to claim 1 contains: the components of the UCST-type thermosensitive hydrogel are: biologically active substances and UCST-type thermosensitive hydrogel materials.

3. The method according to claim 1, characterized in that The bioactive substance in the UCST-type thermosensitive hydrogel loaded with bioactive substances may be hyaluronic acid, sodium hyaluronate, polypeptide, squalane, retinol, glucosamine chondroitin, or essential oil.

4. The UCST-type thermosensitive hydrogel loaded with biologically active substances according to claim 1, characterized in that: The mass fraction of the loaded biologically active substance is 0.01%-1%.

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