Film-forming agent for immunohistochemical pen

Abstract

The invention discloses a film-forming agent for an immunohistochemical pen, which is prepared by taking modified acrylic resin, gamma-nmAl2O3, rosin, isopropanol, acetone, phenyl acetate, liquid paraffin, solvent oil and carbon tetrachloride as raw materials, during preparation, the rosin is firstly added into the solvent oil to be stirred for the first time, insoluble substances are filtered and precipitated after stirring and dissolving, and supernate is reserved; then sequentially adding the isopropanol, the acetone, the phenyl acetate, the liquid paraffin and the carbon tetrachloride into the supernate according to the proportion, and carrying out secondary stirring, so that all the liquid is mixed and dissolved to prepare a single and uniform mixture liquid; and finally, adding gamma-nmAl2O3 into the mixture liquid, continuously stirring in the adding process, and dissolving to obtain a stable suspension, namely the finished product film-forming agent. The film-forming agent disclosed by the invention has good film-forming performance and stability, is suitable for being used by an immunohistochemical pen, and can provide a uniform and transparent film layer with excellent adhesive force.

Description

Technical Field

[0001] The invention relates to immunohistochemical experimental equipment in laboratory equipment technology, and in particular to a film-forming agent used for an immunohistochemical pen. Background Art

[0002] Immunohistochemistry (IHC) is a method for analyzing the presence of specific antigens at the tissue or cell level. By utilizing the principle of highly specific antibodies binding to target antigens, the location of these antigens can be visualized and their qualitative and relative quantitative studies can be performed.

[0003] The immunohistochemistry pen is an important auxiliary tool in immunohistochemistry experiments. It is specifically used to accurately mark tissue sections. This tool has a wide range of mature applications in detecting tumor markers, pathogens, and abnormal proteins, thus facilitating accurate and individualized diagnosis.

[0004] The film-forming agents used in many immunohistochemistry pens in the current technical field lack excellent film-forming properties and hydrophobicity. During the application process, they often show problems such as slow film-forming speed, uneven film distribution, and susceptibility to interference from environmental factors. In addition, these film-forming agents may also affect the accuracy of labeling and the repeatability of experimental results, resulting in unsatisfactory use effects of the corresponding immunohistochemistry pen products. At the same time, these products are usually accompanied by high prices and short shelf lives, which easily lead to waste of immunohistochemistry reagents and cause unnecessary economic losses.

[0005] Based on the above reasons, developing a film-forming agent with better performance is of great significance to improve the accuracy and efficiency of immunohistochemistry experiments. Summary of the invention

[0006] The technical problem solved by the present invention is to provide a film-forming agent for an immunohistochemistry pen, which has a low fluorescence background, good film-forming properties, and good fluidity. It can still have good fluidity when not used for a long time, and can be used to solve the defects in the above technical background.

[0007] The technical problem solved by the present invention is achieved by adopting the following technical solutions:

[0008] A film-forming agent for an immunohistochemistry pen, comprising the following raw materials in parts by weight:

[0009] Modified acrylic resin: 0.5%~2%; γ-nmAl2O3: 0.2%~2%; Rosin: 5%~10%; Isopropyl alcohol: 20%~30%; Acetone: 15%~35%; Phenyl acetate: 8%~12%; Liquid paraffin: 2%~5%; Solvent oil: 5%~25%; Carbon tetrachloride: 5%~15%;

[0010] Prepare in the following manner:

[0011] First, add rosin into solvent oil and stir once. After stirring and dissolving, filter and precipitate insoluble matter and keep the supernatant.

[0012] Adding the modified acrylic resin, isopropyl alcohol, acetone, phenyl acetate, liquid paraffin and carbon tetrachloride in the above proportions to the supernatant in sequence and stirring for a second time, so that all the liquids are miscible to obtain a single uniform mixture liquid;

[0013] Add γ-nmAl2O3 to the above mixture liquid, stir continuously during the adding process, and obtain a stable suspension after dissolution, which is the finished film-forming agent.

[0014] As a further limitation, the particle size of the γ-nmAl2O3 used in the raw material is less than 20 nm.

[0015] As a further limitation, the solvent oil is solvent gasoline, preferably No. 70 solvent oil.

[0016] As a further limitation, when preparing the film-forming agent, the agitator speed during the first stirring is 1500 r / min-2000 r / min, and the agitator speed during the second stirring is 2000 r / min-3000 r / min.

[0017] As a further limitation, when preparing the film-forming agent, γ-nmAl2O3 is added to the mixture liquid and dissolved, and then ultrasonic-assisted treatment is used to avoid precipitation.

[0018] Beneficial effects: The film-forming agent for the immunohistochemistry pen of the present invention has good film-forming property and can quickly form a uniform film layer during the application process, effectively avoiding the problems of slow film-forming speed and uneven film layer distribution of traditional film-forming agents. At the same time, due to the addition of γ~nmAl2O3, the film-forming agent also has good hydrophobicity and can effectively resist the interference of environmental factors, which helps to improve the signal-to-noise ratio of the experiment and ensure the stability and repeatability of the experimental results.

[0019] In addition, the film-forming agent of the present invention has relatively low cost while ensuring performance, and has a long shelf life. Even if it is not used for a long time, it can still maintain good fluidity, which is conducive to the long-term storage and use of immunohistochemistry pens, reducing reagent waste and reducing experimental costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a diagram showing the hydrophobic effect of the film-forming agent after aging in a preferred embodiment of the present invention.

[0021] Figure 2This is a diagram showing the film-forming effect of the film-forming agent of a preferred embodiment of the present invention after aging. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific illustrations and embodiments.

[0023] In the above embodiments, those skilled in the art will understand that, unless otherwise defined, all terms (including technical terms and scientific terms) used herein have the same meaning as the general understanding of those skilled in the art to which this application belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have the same meaning as the meaning in the context of the prior art, and will not be interpreted with an idealized or overly formal meaning unless defined as herein.

[0024] The film-forming agent of the immunohistochemistry pen described in this embodiment is prepared by using the following raw materials:

[0025] Modified acrylic resin, γ-nmAl2O3, rosin, isopropyl alcohol, acetone, phenyl acetate, liquid paraffin, No. 70 solvent oil, carbon tetrachloride, wherein the particle size of γ-nmAl2O3 is screened to be less than 20nm. For the convenience of comparison and description, this embodiment is provided with a plurality of scheme groups with different proportions of components, and the proportions of raw material components of different scheme groups are shown in the following table:

[0026]

[0027]

[0028] The preparation is prepared in the following manner:

[0029] The rosin is dissolved in No. 70 solvent oil. During the mixing process, the stirring speed can be appropriately adjusted to obtain the best mixing effect. In this embodiment, sufficient stirring is ensured at a speed of 1500 to 2000 revolutions per minute until the rosin is completely dissolved.

[0030] Considering that rosin may contain impurities that are insoluble in gasoline, these impurities may affect the fluidity of the final mixture. Therefore, it is necessary to filter out these precipitated insolubles and retain the clear upper liquid.

[0031] Next, the modified acrylic resin, isopropyl alcohol, acetone, phenyl acetate, liquid paraffin and carbon tetrachloride are added to the upper layer of liquid in sequence, and the mixture is stirred at a speed of 2000 to 3000 rpm until all the ingredients are completely mixed to form a uniform solution.

[0032] When adding nano-alumina powder to the above solution, it must be added slowly while stirring continuously to ensure that it is evenly dispersed. After dissolution, use ultrasonic treatment to break up any agglomerates that may be formed. The ultrasonic treatment time should be controlled within 10 to 30 minutes to ensure that the γ-nmAl2O3 particles can be evenly dispersed and avoid precipitation of the film-forming agent during use, thereby preparing a stable suspension.

[0033] In this embodiment, the modified acrylic resin used in the film-forming agent has excellent hydrophobicity and low specific surface energy, and can achieve hydrophobic and water-blocking effects. By adding γ-nmAl2O3, a dispersed powder with a large specific surface area, the hydrophobicity of the film-forming agent can be further enhanced.

[0034] Isopropyl alcohol and acetone are used as base solvents in the film-forming agent. Isopropyl alcohol has stronger non-polarity than many traditional ethanol formulas and has better compatibility with acetone. Gasoline (No. 70 solvent oil) and carbon tetrachloride as co-solvents can effectively help liquid paraffin, rosin and γ-nmAl2O3 to be better dispersed in the system; in addition, adding liquid paraffin can better make the modified acrylic resin form a film, and at the same time improve the stability of the γ-nmAl2O3 system after configuration.

[0035] For the above reasons, the film-forming agent of this embodiment is subjected to quality inspection after preparation to ensure that its key indicators such as film-forming performance, hydrophobicity and fluorescent background meet the standards. In the quality inspection process, the hydrophobicity is evaluated by dropping the film-forming agent onto the glass film and then observing the contact angle from the side; and the film-forming performance is determined by coating the film-forming agent on the glass sheet, then drying the film at room temperature, and then observing the uniformity and transparency of the film layer. Through the technical solution of this embodiment, the following can be obtained: Figure 1 and Figure 2 Typical characteristics shown. In the volatility test, aluminum containers were used for testing, and high-temperature aging was planned in the temperature range of 25-40°C to accelerate the aging process. The test cycle was set to 7 to 15 days.

[0036] And by Figure 1 , Figure 2 It can be seen that the film-forming agent of this embodiment can still maintain good hydrophobicity and film-forming effect after aging. In the result analysis, Scheme 7 performed particularly well, showing excellent water resistance and fluidity, and is therefore very suitable for industrial production.

[0037] In addition, the film-forming agent of this embodiment can still maintain good fluidity after long-term storage, thereby effectively improving the accuracy and efficiency of immunohistochemical experiments.

[0038] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A film-forming agent for an immunohistochemistry pen, characterized in that: The invention comprises the following raw materials in parts by weight: Modified acrylic resin: 0.5% to 2%; γ-nmAl2O3: 0.2 to 2g; rosin: 5% to 10%; isopropyl alcohol: 20% to 30%; acetone: 15% to 35%; phenyl acetate: 8% to 12%; liquid paraffin: 2% to 5%; solvent oil: 5% to 25%; carbon tetrachloride: 5% to 15%; Prepare in the following manner: First, add rosin into solvent oil and stir once. After stirring and dissolving, filter and precipitate insoluble matter and keep the supernatant. Adding isopropanol, acetone, phenyl acetate, liquid paraffin and carbon tetrachloride in the above proportions to the supernatant in sequence and stirring for a second time to make all the liquids miscible to obtain a single uniform mixture liquid; Add γ-nmAl2O3 to the above mixture liquid, stir continuously during the adding process, and obtain a stable suspension after dissolution, which is the finished film-forming agent.

2. The film-forming agent for immunohistochemistry pen according to claim 1, characterized in that: The particle size of γ~nmAl2O3 used in the raw material is less than 20nm.

3. The film-forming agent for immunohistochemistry pen according to claim 1, characterized in that: The solvent oil is No. 70 solvent oil.

4. The film-forming agent for immunohistochemistry pen according to claim 1, characterized in that: When preparing the film-forming agent, the agitator speed during the first stirring is 1500r / min-2000r / min, and the agitator speed during the second stirring is 2000r / min-3000r / min.

5. The film-forming agent for immunohistochemistry pen according to claim 1, characterized in that: When preparing the film-forming agent, γ-nmAl2O3 is added to the mixture liquid and dissolved, and then ultrasonic-assisted treatment is used to avoid precipitation.

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