Automatic liquid sample microorganism rapid enrichment and concentration device

Abstract

The invention belongs to the field of biological detection, and relates to a biological enrichment and detection technology, in particular to a device for enriching and concentrating microorganisms in a liquid sample. The automatic microorganism enrichment and concentration device mainly comprises a power supply module, a suction filtration module, a flushing module, a gas-liquid control module and an input / output display module.

Description

Technical Field

[0001] The present invention relates to the field of biological detection, and to biological enrichment and detection technology, and in particular to a device for enriching and concentrating microorganisms in a liquid sample. Background Art

[0002] When detecting biological particles such as bacteria and viruses in liquid samples, whether the target can be detected depends on its concentration. When the concentration of the target substance in the liquid sample is too low, it usually leads to the problem that the target substance cannot be detected.

[0003] Bio-enrichment technology is to achieve the enrichment of target substances by different methods, so that the concentration of target substances is increased to a detectable level. Common enrichment methods include magnetic bead method, chromatography, filtration method, etc. Among them, the filtration method uses a filter membrane with micro-nano size to intercept the target substance, and passes the liquid sample through the filter membrane under external forces such as gravity, air pressure, and mechanical force, and the target substance is retained on one side of the filter membrane. Finally, the target substance is eluted with a small amount of eluent to achieve the purpose of concentration and enrichment.

[0004] In the prior art, during the elution process, the enrichment equipment usually compresses the eluent and carbon dioxide in a gas cylinder, and uses the carbon dioxide to squeeze the eluent into bubbles during a brief opening. Although foam can be generated, it is impossible to accurately control the amount of eluent and adjust the flushing intensity (air pressure). At the same time, the method of storing high-pressure carbon dioxide and eluent together poses a safety hazard in high temperature, low temperature environments and when impacted.

[0005] In addition, in the prior art, the flushing liquid / high-pressure carbon dioxide is pre-loaded with foam flushing liquid. This method cannot accurately control the amount of flushing liquid and the flushing intensity, and the target recyclables will be incompletely flushed or have reduced activity, and the flushing intensity will decrease as the gas pressure in the bottle decreases. The flushing module in the device of the present invention includes a flushing liquid pump, a flushing liquid arrival sensor, an air pump, a gas storage bottle, an air pressure sensor (integrated on the control panel), and a bubbler. The volume of the flushing liquid can be set and automatically controlled. After the flushing liquid flows into the flushing pipeline, the high-pressure gas is used to push the flushing liquid forward along the pipeline to flush and recover the target substance. The flushing liquid volume and flushing pressure can be accurately controlled to ensure the enrichment effect.

[0006] In the current sample recovery process, manual / mechanical repeated washing methods are used, and only filter membranes are used as interception methods. This method may cause the target recyclate to fail to be intercepted, be embedded in the filter pores, break in shape and lose activity, and the target recyclate recovery rate is low. In order to solve these problems, self-developed magnetic beads or enriched magnetic particles are added to the sample, so that the target recyclate is adsorbed on the magnetic beads and wrapped by them, increasing the volume to prevent interception failure, and avoid embedding in the filter pores and breaking in shape, which can improve the recovery rate and maintain activity. Summary of the invention

[0007] The purpose of the present invention is to provide an automated liquid sample microorganism rapid enrichment and concentration device.

[0008] The microorganism automatic enrichment and concentration device of the present invention is mainly composed of a power supply module, a filtration module, a flushing module, a gas-liquid control module, and an input-output display module.

[0009] The power supply module includes an AC power plug or a rechargeable battery.

[0010] The power supply module is connected to the gas-liquid control module through a circuit, and the gas-liquid control module is connected to the filtration module, the flushing module, and the input-output module through a circuit.

[0011] Among them, the filtration module includes two versions:

[0012] (1) The negative pressure bottle version filtration module comprises: a filtration pump (2), a negative pressure bottle (1), a first solenoid valve (11), and a second solenoid valve (12);

[0013] (2) The direct filtration version filtration module includes: a filtration pump (2).

[0014] The connections of the various components are as follows:

[0015] Negative pressure bottle version filtration module: the air inlet of the filtration pump (2) is connected to the air outlet of the negative pressure bottle (1) through an air pipe, the water inlet of the negative pressure bottle (1) is connected to the water outlet of the second solenoid valve (12), and the air outlet of the first solenoid valve (11), the water inlet of the second solenoid valve (12) and the filtration port of the ultrafiltration tube (3) are connected through a three-way joint.

[0016] Direct filtration version filtration module: the water inlet of the filtration pump (2) is connected to the filtration port of the ultrafiltration tube (3) through a water pipe; the water outlet of the filtration pump (2) is connected to the water pipe, and the water pipe is placed in the waste liquid bottle (1).

[0017] Among them, flushing module:

[0018] The negative pressure bottle version has the same flushing module as the direct filtration version, and includes three parts: the upper liquid part, the high-pressure gas part, and the mixing part.

[0019] The liquid supply part comprises: a flushing liquid bottle (4) and a liquid supply pump (5);

[0020] High-pressure gas part: an air pump (8), a second sensor (9), a gas storage bottle (10), and a third solenoid valve (13);

[0021] Mixing part: Bubbler (6).

[0022] The connections of the various components are as follows:

[0023] Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to a three-way joint through a hose.

[0024] High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline. The air outlet of the gas storage bottle (10) and the air inlet of the third electromagnetic valve (13) are connected through a pipeline.

[0025] The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected through a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler.

[0026] The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

[0027] Among them, gas-liquid control module: control circuit board.

[0028] The connections of the various components are as follows:

[0029] The gas-liquid control module is connected to the suction pump (2), the first solenoid valve (11), the second solenoid valve (12), the upper liquid pump (5), the inflation pump (8), the third solenoid valve (13), the first sensor (7), and the second sensor (9) through a circuit.

[0030] Among them, the input and output display module: a touch display screen (17).

[0031] The touch display screen (17) is connected to the gas-liquid control module via a circuit.

[0032] The automated liquid sample microorganism rapid enrichment and concentration device of the present invention is divided into: a negative pressure bottle version automated liquid sample microorganism enrichment and concentration device, and a direct filtration version automated liquid sample microorganism enrichment and concentration device.

[0033] The negative pressure bottle version of the automated liquid sample microbial enrichment and concentration device is composed of the following components:

[0034] The air inlet of the filtration pump (2) is connected to the air outlet of the negative pressure bottle (1) through an air pipe, the water inlet of the negative pressure bottle (1) is connected to the water outlet of the second solenoid valve (12), and the air outlet of the first solenoid valve (11), the water inlet of the second solenoid valve (12) and the filtration port of the ultrafiltration tube (3) are connected through a three-way joint.

[0035] Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to a three-way joint through a hose.

[0036] High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline.

[0037] The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected via a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler (6).

[0038] The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

[0039] The enrichment method of the negative pressure bottle-type automated enrichment and concentration device of the present invention comprises the following steps:

[0040] The instrument is started by turning on the power supply, and the suction pump (2) is started, so that the negative pressure bottle (1) is in a negative pressure state; under the action of negative pressure, the liquid sample enters the negative pressure bottle (1) from the liquid outlet through the pipeline via the ultrafiltration tube (3); after the filtration is completed, the suction pump (2) is stopped, the first electromagnetic valve (11) is opened to remove the negative pressure of the negative pressure bottle (1), and then the first electromagnetic valve (11) is closed; the upper liquid pump (5) is started, and the flushing liquid is pumped from the flushing liquid bottle (4) into the pipeline through the upper liquid pump (5); when the flushing liquid reaches the first sensor (7), the upper liquid pump (5) is stopped, the air pump (8) is started to inflate, and the gas storage bottle ( 10) The pressure rises, and the second sensor (9) senses that the pressure in the gas storage bottle (10) reaches the set value. The control program controls the air pump (8) to stop, closes the second solenoid valve (12) and opens the third solenoid valve (13). The high-pressure gas pushes the flushing liquid in the pipeline through the bubbler (6). The foam-like flushing liquid formed in the bubbler (6) enters the ultrafiltration tube (3) through the flushing port to flush the target substance retained by the ultrafiltration membrane. The flushing liquid is discharged from the ultrafiltration tube sampling port and recovered. After the gas pressure in the gas storage bottle (10) returns to normal pressure, the third solenoid valve (13) is closed.

[0041] The direct filtration version of the automated liquid sample enrichment and concentration device of the present invention is formed by connecting the following components:

[0042] The water inlet of the suction filter pump (2) is connected to the suction filter port of the ultrafiltration tube (3) through a water pipe, and the water outlet of the suction filter pump (2) is connected to the water pipe, which is placed in the waste liquid bottle (1).

[0043] Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to a three-way joint through a hose.

[0044] High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline.

[0045] The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected via a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler (6).

[0046] The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

[0047] The enrichment method of the direct filtration version of the automated enrichment and concentration device of the present invention comprises the following steps:

[0048] Turn on the power to start the instrument, the filtration pump (2) starts, the liquid sample is discharged from the liquid outlet through the ultrafiltration tube (3) into the filtration pump (2), and discharged into the waste liquid bottle (1) through the liquid outlet of the filtration pump (2); after the filtration is completed, stop the filtration pump (2); start the upper liquid pump (5), and the flushing liquid is pumped from the flushing liquid bottle (4) into the pipeline through the upper liquid pump (5); when the flushing liquid reaches the first sensor (7), the upper liquid pump (5) stops, the inflation pump (8) starts to inflate, the pressure of the gas storage bottle (10) increases, and the second sensor (9) After sensing that the pressure in the gas storage bottle (10) has reached the set value, the control program controls the air pump (8) to stop; the third solenoid valve (13) is opened, and the high-pressure gas pushes the flushing liquid in the pipeline through the bubbler (6). The foam-like flushing liquid formed in the bubbler (6) enters the ultrafiltration tube (3) through the flushing port to flush the target substance retained by the ultrafiltration membrane, and the flushing liquid is discharged from the ultrafiltration tube sampling port to be recovered; after the gas pressure in the gas storage bottle (10) returns to normal pressure, the third solenoid valve (13) is closed.

[0049] Compared with the prior art, the present invention has the following beneficial effects:

[0050] 1) The pressure of the high-pressure gas used to drive the flushing is adjustable to avoid damage to the structure of pathogenic microorganisms caused by large impacts and to increase the activity of the recovered target substances; to avoid the phenomenon of gas pressure decreasing during the flushing process when the high-pressure carbon dioxide pre-filled flushing liquid is used, and to ensure that the flushing intensity is consistent each time.

[0051] 2) The flushing liquid contains a surfactant and a defoaming agent. Under the impetus of high-pressure gas, it is mixed through a bubbler to produce foam. The flushing liquid flushes the inside of the filter membrane in the form of foam, increasing the contact area and contact time between the flushing liquid and the filter membrane.

[0052] 3) High-pressure gas is not stored together with the flushing liquid, which is safer; and the flushing liquid dosage and flushing pressure can be set separately according to the use requirements, which is suitable for different types of samples and can improve the enrichment recovery rate and the activity of the enriched samples.

[0053] 4) Independent bubbler, the air pressure inside and outside the foam is close, and the foam bursts gently, which reduces the energy release during bursting, reduces the activity of microorganisms, and reduces the generation of aerosols.

[0054] 5) Filters are available in a variety of different specifications, with different membrane pore sizes, filter diameters, membrane surface treatment forms, and membrane retention areas. The membranes can be hollow fiber membranes or flat membranes, and can be selected based on different sample types and sample volumes to ensure enrichment efficiency.

[0055] 6) Use a negative pressure bottle to hold the sample to filter out the waste liquid. The float of the negative pressure bottle rises as the liquid level rises. After a certain height, the liquid will not be able to enter the negative pressure bottle, preventing the liquid in the negative pressure bottle from being too full and overflowing onto the test bench.

[0056] 7) The device has self-developed control software and hardware, which is simple to operate, stable in operation, highly safe, and supports functional expansion. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 , Gas-liquid pipeline connection diagram of negative pressure bottle version automated liquid sample microbial rapid enrichment and concentration device

[0058] Figure 2 , Direct filtration version of the automated liquid sample microbial rapid enrichment and concentration device gas-liquid pipeline connection diagram

[0059] Figure 3 Schematic diagram of the automated liquid sample microbial rapid enrichment and concentration device

[0060] Figure 4 Schematic diagram of the automated liquid sample microbial rapid enrichment and concentration device

[0061] Figure 5 Schematic diagram of the automated liquid sample microbial rapid enrichment and concentration device

[0062] Figure 6 , negative pressure bottle version of the automated liquid sample microbial rapid enrichment and concentration device module relationship diagram

[0063] Figure 7 , Direct filtration version of the automated liquid sample microbial rapid enrichment and concentration device module relationship diagram

[0064] In the figure:

[0065] Negative pressure bottle 1, suction pump 2, ultrafiltration tube 3, flushing liquid bottle 4, upper liquid pump 5, bubbler 6, first sensor 7, inflation pump 8, second sensor 9, gas storage bottle 10, first solenoid valve 11, second solenoid valve 12, third solenoid valve 13, control circuit board 14, power supply module 15, switch 16, touch display screen 17. DETAILED DESCRIPTION

[0066] The present invention is further illustrated by the following examples.

[0067] The product and preparation method of the present invention are further described below in conjunction with specific examples, but the present invention is not limited to the following examples. The methods are conventional methods unless otherwise specified. The raw materials can be obtained from public commercial channels unless otherwise specified.

[0068] Example 1: Negative pressure bottle version of automated liquid sample microbial rapid enrichment and concentration device

[0069] like Figure 1 As shown, the negative pressure bottle version of the automated liquid sample microorganism rapid enrichment and concentration device is composed of the following components:

[0070] The air inlet of the filtration pump (2) is connected to the air outlet of the negative pressure bottle (1) through an air pipe, the water inlet of the negative pressure bottle (1) is connected to the water outlet of the second solenoid valve (12), and the air outlet of the first solenoid valve (11), the water inlet of the second solenoid valve (12) and the filtration port of the ultrafiltration tube (3) are connected through a three-way joint.

[0071] Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to a three-way joint through a hose.

[0072] High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline.

[0073] The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected via a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler (6).

[0074] The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

[0075] The enrichment method of the negative pressure bottle-type automated enrichment and concentration device of the present invention comprises the following steps:

[0076] The instrument is started by turning on the power supply, and the suction pump (2) is started, so that the negative pressure bottle (1) is in a negative pressure state; under the action of negative pressure, the liquid sample enters the negative pressure bottle (1) from the liquid outlet through the pipeline via the ultrafiltration tube (3); after the filtration is completed, the suction pump (2) is stopped, the first electromagnetic valve (11) is opened to remove the negative pressure of the negative pressure bottle (1), and then the first electromagnetic valve (11) is closed; the upper liquid pump (5) is started, and the flushing liquid is pumped from the flushing liquid bottle (4) into the pipeline through the upper liquid pump (5); when the flushing liquid reaches the first sensor (7), the upper liquid pump (5) is stopped, the air pump (8) is started to inflate, and the gas storage bottle ( 10) The pressure rises, and the second sensor (9) senses that the pressure in the gas storage bottle (10) reaches the set value. The control program controls the air pump (8) to stop, closes the second solenoid valve (12) and opens the third solenoid valve (13). The high-pressure gas pushes the flushing liquid in the pipeline through the bubbler (6). The foam-like flushing liquid formed in the bubbler (6) enters the ultrafiltration tube (3) through the flushing port to flush the target substance retained by the ultrafiltration membrane. The flushing liquid is discharged from the ultrafiltration tube sampling port and recovered. After the gas pressure in the gas storage bottle (10) returns to normal pressure, the third solenoid valve (13) is closed.

[0077] Example 2: Direct filtration version of automated solid sample enrichment and concentration device

[0078] like Figure 2 As shown in the figure, the direct filtration version of the automated liquid sample enrichment and concentration device is composed of the following components:

[0079] The water inlet of the suction filter pump (2) is connected to the suction filter port of the ultrafiltration tube (3) through a water pipe, and the water outlet of the suction filter pump (2) is connected to the water pipe, which is placed in the waste liquid bottle (1).

[0080] Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to a three-way joint through a hose.

[0081] High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline.

[0082] The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected via a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler (6).

[0083] The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

[0084] The enrichment method of the direct filtration version of the automated enrichment and concentration device of the present invention comprises the following steps:

[0085] Turn on the power to start the instrument, the filtration pump (2) starts, the liquid sample is discharged from the liquid outlet through the ultrafiltration tube (3) into the filtration pump (2), and discharged into the waste liquid bottle (1) through the liquid outlet of the filtration pump (2); after the filtration is completed, stop the filtration pump (2); start the upper liquid pump (5), and the flushing liquid is pumped from the flushing liquid bottle (4) into the pipeline through the upper liquid pump (5); when the flushing liquid reaches the first sensor (7), the upper liquid pump (5) stops, the inflation pump (8) starts to inflate, the pressure of the gas storage bottle (10) increases, and the second sensor (9) After sensing that the pressure in the gas storage bottle (10) has reached the set value, the control program controls the air pump (8) to stop; the third solenoid valve (13) is opened, and the high-pressure gas pushes the flushing liquid in the pipeline through the bubbler (6). The foam-like flushing liquid formed in the bubbler (6) enters the ultrafiltration tube (3) through the flushing port to flush the target substance retained by the ultrafiltration membrane, and the flushing liquid is discharged from the ultrafiltration tube sampling port to be recovered; after the gas pressure in the gas storage bottle (10) returns to normal pressure, the third solenoid valve (13) is closed.

[0086] Example 3: Circuit connection of negative pressure bottle version automated liquid sample microorganism rapid enrichment and concentration device Figure 6 As shown, the power supply module is connected to the switch and the gas-liquid control module through the circuit, and the gas-liquid control module is connected to the first sensor 7, the second sensor 9, the filtration pump 2, the inflation pump 8, the touch display screen 17, the upper liquid pump 5, the first solenoid valve 11, the second solenoid valve 12, and the third solenoid valve 13 through the circuit.

[0087] Example 4: Circuit connection of the direct filtration version of the automated solid sample enrichment and concentration device

[0088] like Figure 7 As shown, the power supply module is connected to the switch and the gas-liquid control module through the circuit, and the gas-liquid control module is connected to the first sensor 7, the second sensor 9, the upper liquid pump 5, the filtration pump 2, the inflation pump 8, the touch display screen 17, and the third solenoid valve 13 through the circuit.

Claims

1. An automated liquid sample microorganism rapid enrichment and concentration device, mainly composed of a power supply module, a filtration module, a flushing module, a gas-liquid control module, and an input-output display module.

2. The device according to claim 1, characterized in that in, Power supply module, including AC power plug or rechargeable battery; The power supply module is connected to the gas-liquid control module through electric wires, and the gas-liquid control module is connected to the filtration module, the flushing module, and the input-output module through electric wires.

3. The device according to claim 1, It is characterized in that Among them, the filtration module includes two versions: The negative pressure bottle version filtration module comprises: a filtration pump (2), a negative pressure bottle (1), a first solenoid valve (11), and a second solenoid valve (12); The direct filtration version filtration module comprises: a filtration pump (2); The connections of the various components are as follows: Negative pressure bottle version filtration module: the air inlet of the filtration pump (2) is connected to the air outlet of the negative pressure bottle (1) through an air pipe, the water inlet of the negative pressure bottle (1) is connected to the water outlet of the second solenoid valve (12), and the air outlet of the first solenoid valve (11), the water inlet of the second solenoid valve (12) and the filtration port of the ultrafiltration tube (3) are connected through a three-way joint; Direct filtration version filtration module: the water inlet of the filtration pump (2) is connected to the filtration port of the ultrafiltration tube (3) through a water pipe; the water outlet of the filtration pump (2) is connected to the water pipe, and the water pipe is placed in the waste liquid bottle (1).

4. The device according to claim 1, It is characterized in that Among them, flushing module: The negative pressure bottle version has the same flushing module as the direct filtration version, and consists of three parts: the upper liquid part, the high-pressure gas part, and the mixing part; The liquid supply part comprises: a flushing liquid bottle (4) and a liquid supply pump (5); High-pressure gas part: an air pump (8), a second sensor (9), a gas storage bottle (10), and a third solenoid valve (13); Mixing part: bubbler (6); The connections of the various components are as follows: Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to a three-way joint through a hose; High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline; The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected via a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module; the sensor 1 is located between the three-way joint and the bubbler.

5. The device according to claim 1, characterized in that The negative pressure bottle version of the automated liquid sample microorganism rapid enrichment and concentration device is composed of the following components: The air inlet of the suction filter pump (2) is connected to the air outlet of the negative pressure bottle (1) through an air pipe, the water inlet of the negative pressure bottle (1) is connected to the water outlet of the second electromagnetic valve (12), the air outlet of the first electromagnetic valve (11), the water inlet of the second electromagnetic valve (12) and the suction filter port of the ultrafiltration tube (3) are connected through a three-way joint. Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to the three-way joint through a hose. High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline. The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected by a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler (6). The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

6. The device according to claim 1, characterized in that The direct filtration version of the automated liquid sample microbial rapid enrichment and concentration device is composed of the following components: The water inlet of the suction filter pump (2) is connected to the suction filter port of the ultrafiltration tube (3) through a water pipe, and the water outlet of the suction filter pump (2) is connected to the water pipe, which is placed in the waste liquid bottle (1). Liquid supply part: the flushing liquid bottle (4) is connected to the liquid inlet of the liquid supply pump (5) through a hose, and the liquid outlet of the liquid supply pump (5) is connected to the three-way joint through a hose. High-pressure gas part: the air pump (8), the second sensor (9), and the air inlet of the gas storage bottle (10) are connected to a three-way joint through a pipeline, and the air outlet of the gas storage bottle (10) and the air inlet of the third solenoid valve (13) are connected through a pipeline. The liquid outlet of the upper liquid pump (5), the gas outlet of the third solenoid valve (13), and the inlet end of the bubbler (6) are connected by a three-way joint, thereby connecting the upper liquid part, the high-pressure gas part, and the mixing part of the flushing module. The first sensor (7) is located between the three-way joint and the bubbler (6). The discharge end of the bubbler (6) is connected to the flushing port of the ultrafiltration tube (3) through a pipeline.

7. An enrichment method using the negative pressure bottle-type automated enrichment and concentration device as claimed in claim 5, It is characterized in that The instrument is started by turning on the power supply, and the suction pump (2) is started, so that the negative pressure bottle (1) is in a negative pressure state; under the action of negative pressure, the liquid sample enters the negative pressure bottle (1) from the liquid outlet through the pipeline via the ultrafiltration tube (3); after the filtration is completed, the suction pump (2) is stopped, the first electromagnetic valve (11) is opened to remove the negative pressure of the negative pressure bottle (1), and then the first electromagnetic valve (11) is closed; the upper liquid pump (5) is started, and the flushing liquid is pumped from the flushing liquid bottle (4) into the pipeline through the upper liquid pump (5); when the flushing liquid reaches the first sensor (7), the upper liquid pump (5) is stopped, the air pump (8) is started to inflate, and the gas storage bottle ( 10) The pressure rises, and the second sensor (9) senses that the pressure in the gas storage bottle (10) reaches the set value. The control program controls the air pump (8) to stop, closes the second solenoid valve (12) and opens the third solenoid valve (13). The high-pressure gas pushes the flushing liquid in the pipeline through the bubbler (6). The foam-like flushing liquid formed in the bubbler (6) enters the ultrafiltration tube (3) through the flushing port to flush the target substance retained by the ultrafiltration membrane. The flushing liquid is discharged from the ultrafiltration tube sampling port and recovered. After the gas pressure in the gas storage bottle (10) returns to normal pressure, the third solenoid valve (13) is closed.

8. An enrichment method using the direct filtration automated enrichment and concentration device as claimed in claim 6, characterized in that: Turn on the power to start the instrument, the filtration pump (2) starts, the liquid sample is discharged from the liquid outlet through the ultrafiltration tube (3) into the filtration pump (2), and discharged into the waste liquid bottle (1) through the liquid outlet of the filtration pump (2); after the filtration is completed, stop the filtration pump (2); start the upper liquid pump (5), and the flushing liquid is pumped from the flushing liquid bottle (4) into the pipeline through the upper liquid pump (5); when the flushing liquid reaches the first sensor (7), the upper liquid pump (5) stops, the inflation pump (8) starts to inflate, the pressure of the gas storage bottle (10) increases, and the second sensor (9) After sensing that the pressure in the gas storage bottle (10) has reached the set value, the control program controls the air pump (8) to stop; the third solenoid valve (13) is opened, and the high-pressure gas pushes the flushing liquid in the pipeline through the bubbler (6). The foam-like flushing liquid formed in the bubbler (6) enters the ultrafiltration tube (3) through the flushing port to flush the target substance retained by the ultrafiltration membrane, and the flushing liquid is discharged from the ultrafiltration tube sampling port to be recovered; after the gas pressure in the gas storage bottle (10) returns to normal pressure, the third solenoid valve (13) is closed.

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