Preparation method of barley grass nutrient solution

Abstract

The invention provides a barley grass nutrient solution preparation method which comprises the following steps: S101, constructing an experimental subject and a corresponding sample set, S102, detecting specific elements in the sample set, S103, calculating the apparent absorptivity of livestock to the specific elements and the total amount of the specific elements required for the growth of the livestock, and S104, performing experimental determination on the total amount of the specific elements required for the growth of the livestock under a clear water culture condition. The method comprises the following steps: S105, determining the content of specific elements of barley seeds after the barley seeds are converted into barley grass, S105, determining the enrichment capacity and the maximum critical concentration of the barley grass on the specific elements under the culture condition of a nutrient solution through experiments, S106, calculating the concentration range of the specific elements in the nutrient solution, and S107, calculating the optimal concentration of the specific elements in the nutrient solution. The method has the beneficial effects that the green barley grass feed is taken as a carrier, the trace elements in the animal product are rapidly supplemented by detecting the trace elements in the animal product, calculating the optimal concentration of the trace elements in the nutrient solution and timely adjusting the preparation of the nutrient solution, and finally, the quality and efficiency of the animal product are improved.

Description

Technical Field

[0001] The invention relates to the field of plant cultivation technology and animal nutrition, and in particular to a method for preparing a nutrient solution for barley grass. Background Art

[0002] In the pasture breeding process, livestock diet is a key factor in determining the output and quality of livestock products. However, in actual production, pastures usually formulate feeding strategies based on the experience of breeders and nutritionists. For example, through years of feeding experiments, pastures have combined the growth habits of livestock breeds to explore a set of feeding methods suitable for the pasture, including diet formula, feeding amount, feeding frequency, etc., which belong to the category of empirical knowledge.

[0003] The main raw materials of traditional livestock diets are significantly affected by the seasons, so livestock diets also need to be adjusted with the seasons, which to a certain extent restricts the further intensive development of animal husbandry. For example, in winter, due to the lack of green feed, the proportion of hay and silage fed increases, which may cause stress reactions, fluctuations in livestock nutrient intake and digestion levels, or even a significant decrease, resulting in a lack of specific nutrients and trace elements, and ultimately leading to a decrease in livestock product output and quality, resulting in economic losses.

[0004] The typical way to solve the problem of trace element deficiency and nutritional balance in livestock is to directly add trace element compounds to the feed. This method has a low absorption rate of trace elements by livestock, and the dosage and actual effect of trace element compounds are difficult to measure, making it difficult to achieve quantitative customization and high dynamic response of trace element supplementation programs.

[0005] In recent years, the cultivation of barley grass and the use of barley grass green feed in livestock diets have gradually become popular in the breeding industry. By applying nutrient solution containing trace elements during the cultivation of barley grass and systematically building a digital configuration plan and evaluation system for the composition of the nutrient solution, the quality and efficiency of livestock products can be improved, further promoting the high-quality development of animal husbandry. Summary of the invention

[0006] In view of the problems existing in the prior art, the present invention provides a method for preparing a barley grass nutrient solution, which is used to cultivate barley grass enriched with specific elements and feed it to livestock, thereby solving the problem of specific element deficiency in livestock products, improving the quality and efficiency of livestock products, and meeting the standards of high-quality livestock products.

[0007] In order to achieve the above object, the present invention provides a method for preparing a nutrient solution of barley grass, the steps of the method are as follows:

[0008] S101: Construct an experimental object set of the pasture to be tested, randomly sample the livestock products of the experimental objects, register the sources of the samples, and form a sample set to be tested.

[0009] S102: Detect the content of specific elements in the samples to be tested, and grade the quality of livestock products based on the element content indicators.

[0010] S103: Calculate the apparent absorption rate of the experimental object to the specific element and the total amount of the specific element required for its own growth.

[0011] S104: Experimental determination of the content of specific elements in barley seeds after conversion to barley grass under clear water culture conditions.

[0012] S105: Experimentally determine the enrichment capacity and maximum critical concentration of specific elements of barley grass under nutrient solution culture conditions.

[0013] S106: Calculate the concentration range of a specific element in the nutrient solution.

[0014] S107: Calculate the optimum concentration of specific elements in the nutrient solution.

[0015] The present invention provides a method for preparing a nutrient solution of barley grass, wherein the culturing process of the barley grass and the steps for determining the content of specific elements are as follows:

[0016] S201: Select plump and healthy barley seeds, soak them in clean water for 15 hours, keep the water temperature at 25-28℃ and aerate them to ensure that the seeds absorb enough water; after soaking, take out the seeds and control the water for 3 hours, and soak them again for 2-3 hours, keep the water temperature at 25-28℃ and aerate them; after soaking, take out the seeds and spread them flat on the culture rack to keep the surface of the seeds moist.

[0017] S202: Use an automated control system to monitor parameters such as temperature, humidity, and light intensity. The temperature is controlled at 18-25°C and the humidity is 75%-95%. Cultivate in the dark for the first three days after sowing. On the fourth day, turn on the plant supplementary light for supplementary lighting. The light intensity is 2000-3000 lux to provide a good growth environment for barley grass until the seventh day.

[0018] S203: Whole-plant sampling of barley grass on the seventh day of growth, with a sample weight of no less than 300g, is conducted for specific element concentration testing using near-infrared testing.

[0019] The present invention provides a method for preparing a nutrient solution for barley grass. The calculation process of the apparent absorption rate of a specific element by an experimental object and the content of the element used for its own growth in step S103 is as follows:

[0020] S301: Calculate the intake of specific elements by the experimental subjects during the feeding period, the formula is as follows:

[0021] Where Ki is the specific element concentration in the diet of experimental subject i, N i is the total weight of food consumed by experimental subject i during the feeding period, and n is the number of experimental subjects.

[0022] S302: Calculate the content of specific elements that are not utilized by the experimental object during the feeding cycle, the formula is as follows:

[0023] in is the concentration of specific elements in the feces of experimental subject i, W i is the total mass of feces of experimental subject i during the feeding period; is the concentration of a specific element in the urine of subject i, V i is the total urine mass of experimental subject i during the feeding period; n is the number of experimental subjects.

[0024] S303: Calculate the apparent absorption rate of the experimental object to a specific element, the formula is as follows:

[0025] in is the calculation result of step S301, is the calculation result of step S302. Thus, the apparent absorption rate of livestock to the specific element is obtained.

[0026] S304: Calculate the content of specific elements in the livestock product samples corresponding to the experimental object, the formula is as follows:

[0027] Where C is the concentration of a specific element in the livestock product sample, and W is the mass of the livestock product sample.

[0028] S305: Calculate the amount of specific elements required for the growth of the experimental object. The formula is as follows:

[0029] The present invention provides a method for preparing a nutrient solution of barley grass. In the step S105, under the nutrient solution culture conditions, the calculation process of the enrichment capacity and the maximum critical concentration of barley grass for a specific element is as follows:

[0030] S401: Cultivating barley grass in nutrient solutions containing different concentrations of specific elements.

[0031] S402: Whole-plant sampling of barley grass on the seventh day after cultivation in nutrient solutions with different concentrations of specific elements is carried out, with the sample mass not less than 300g, for specific element concentration testing. Based on the growth trend and test results, the relationship between the specific element concentration in the nutrient solution and the specific element concentration in the barley grass (dry sample) is obtained.

[0032] S403: Obtaining the inflection point value of the relationship between the concentration of the specific element in the nutrient solution and the concentration of the specific element in the barley grass (dry sample), which is the maximum critical concentration of the specific element that the barley grass can withstand .

[0033] S404: Calculate the enrichment capacity of barley grass for specific elements. The formula is as follows:

[0034] Where △c is the concentration difference of specific elements in the cultivated barley grass, W D is the fresh grass yield, W W is the water content of barley grass, △C is the concentration difference of specific elements in the nutrient solution, and V is the mass of the applied nutrient solution.

[0035] The present invention provides a method for preparing a nutrient solution of barley grass. The step S106 calculates the concentration range of a specific element in the nutrient solution of barley grass, and the steps are as follows:

[0036] S501: According to the quality grading method of trace element content in livestock products, the lower limit concentration of trace element content that meets the quality livestock product standards is calculated as C D ， The upper limit concentration is C U 。

[0037] S502: For the experimental subjects that do not meet the quality livestock product standards, calculate the lower limit of the content of specific trace elements in the barley grass nutrient solution required by them, the formula is as follows:

[0038] S503: For the experimental subjects that do not meet the quality livestock product standards, calculate the upper limit of the content of specific trace elements in the barley grass nutrient solution required by them, the formula is as follows:

[0039] in W C The total amount of specific elements required for the growth of experimental subjects to produce high-quality livestock products; W c The total amount of specific elements required for the growth of experimental subjects that failed to produce high-quality livestock products; C D 、 C U is the result of step S501; C N concentrations of specific elements in products that do not meet quality livestock product standards; W For the production of high-quality livestock products, W Nis the product output that does not meet the quality livestock product standards; c1 is the specific element concentration after barley seeds are converted into barley grass under clear water culture conditions; a is the amount of barley grass fed in the diet; W W is the water content of barley grass; E 2 is the apparent absorption rate of specific elements by the experimental subjects that failed to produce high-quality livestock products; v is the amount of nutrient solution poured each time; p is the frequency of nutrient solution pouring; and P is the enrichment capacity of barley grass for specific elements.

[0040] S504: The concentration range of specific elements in the barley grass nutrient solution is calculated as a set With Collection The intersection of , where c h It is the maximum critical concentration of a specific element that barley grass can withstand.

[0041] The present invention provides a method for preparing a nutrient solution for barley grass. The step S107 obtains the optimum concentration of the nutrient solution for a specific element based on the cost and benefit conditions. The steps are as follows:

[0042] S601: Calculate the cost of the nutrient solution using the following formula:

[0043] in c is the concentration of a specific element in the nutrient solution, v is the volume of the nutrient solution, u The unit price of a specific element raw material.

[0044] S602: Calculate the economic benefits of not adding nutrient solution, the formula is as follows:

[0045] Among them, q0 is the output of livestock products when no nutrient solution is added, and u0 is the unit price of livestock products when no nutrient solution is added.

[0046] S603: Calculate the economic benefits of adding nutrient solution. The formula is as follows:

[0047] Among them, q1 is the output of livestock products when nutrient solution is added, and u1 is the unit price of livestock products when nutrient solution is added.

[0048] S604 calculates the incremental benefit of adding nutrient solution, and the formula is as follows:

[0049] in S 0 For the economic benefits of not adding nutrient solution,S 2 To increase the economic benefits of adding nutrient solution, S 1 The cost of the nutrient solution.

[0050] S605: Through market research, the relationship between the concentration of specific elements c and the unit price of livestock products u1 is obtained, which is expressed as follows: .

[0051] S606: Substitute the formula of S605 into the formula of S604 to obtain the relationship between the benefit and the element concentration in the nutrient solution:

[0052] in .

[0053] S607: Calculate S in the interval The maximum value of c is the concentration of specific elements in the nutrient solution corresponding to the highest increase in livestock product benefits from adding nutrient solution, that is, the optimal concentration.

[0054] Furthermore, the present invention provides a method for preparing a nutrient solution for barley grass. The nutrient solution in step S401 for culturing barley grass can be applied by adding the nutrient solution to a water storage device for hydroponics, mist culture, soil culture, or substrate culture, or by adding effective ingredients of the nutrient solution to a pulp raw material to make culture paper, and laying the paper containing the nutrient solution ingredients on a culture vessel before culture, or by making the effective ingredients of the nutrient solution into a seed coating.

[0055] Furthermore, the present invention provides a method for preparing a nutrient solution for barley grass, wherein step S107 can not only analyze a single specific element to obtain the optimum concentration for preparing the nutrient solution, but also analyze multiple specific elements. One method for analyzing multiple specific elements is to perform step S107 calculation on each of the multiple elements, and then prepare each element into a mixed nutrient solution according to its optimum concentration; another method is to expand the formula in step S601 to , expand the formula in step S605 to ,in is the vector of the concentration of each element in the nutrient solution, is the unit price vector of each element raw material, and then the calculation of steps S606 and S607 is performed to obtain The optimal value of The optimal configuration is a mixed nutrient solution.

[0056] Furthermore, the present invention provides a method for preparing a barley grass nutrient solution, wherein the specific element may be one of trace elements required for livestock growth, such as calcium, iron, zinc, and selenium, and the livestock product may be one of meat, eggs, and milk.

[0057] Beneficial effects of the present invention:

[0058] 1: The method for preparing the nutrient solution for barley grass provided by the present invention has multiple advantages. It can realize the rapid cultivation of barley grass, improve the quality, yield and trace element content of barley grass, on the one hand meet the demand of pastures for green feed, and on the other hand provide a method for pastures to efficiently and customizedly supplement trace elements for livestock, promote balanced nutrition and healthy growth of livestock, improve the yield and quality of livestock products, further meet market demand, and have good economic and social benefits.

[0059] 2: The present invention provides a digitized animal nutrition program, which is based on experimental statistical results such as the apparent absorption rate of nutrients by livestock and the ability of barley grass to enrich nutrients, takes improving the production efficiency of livestock products as the optimization goal, calculates the optimal concentration of the nutrient solution used for barley grass cultivation, and achieves the purpose of reducing costs and increasing efficiency.

[0060] 3: The present invention provides a dynamically responsive green feed trace element content formulation scheme, which uses barley grass as a carrier, detects trace elements in livestock products, and timely adjusts the proportion of trace elements in the nutrient solution to achieve rapid supplementation of trace elements in livestock products. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following is a brief introduction to the drawings required for use in the embodiments or the prior art description. The drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0062] Figure 1 A flow chart of a method for preparing a nutrient solution for barley grass;

[0063] Figure 2 Flow chart for calculating apparent absorbance and total amount of elements required for growth;

[0064] Figure 3 Flow chart for determining element enrichment capacity and maximum critical concentration;

[0065] Figure 4 Flow chart for calculating the range of element concentrations in the nutrient solution;

[0066] Figure 5 Flow chart for calculating the optimal concentration of elements in the nutrient solution. DETAILED DESCRIPTION

[0067] The calcium content in dairy cows is one of the key factors in maintaining their health and production performance. Calcium is the main component of the cow's bone and tooth structure, and is essential for maintaining nerve and muscle function, blood coagulation, cell signaling, and many other physiological processes. At the same time, the calcium content in dairy products is also an important factor in measuring the quality of dairy products.

[0068] The present invention provides a method for preparing a nutrient solution of barley grass, wherein the barley variety is selected as Ganbi No. 8, the concentration of the nutrient solution of barley grass is calculated, the barley grass enriched with calcium is cultivated, and the barley grass is fed to dairy cows to improve the quality and efficiency of dairy products, and the specific steps are as follows:

[0069] S101: Construct the experimental object set of the tested pasture, randomly select 5 groups of dairy cows in the middle of lactation, with similar average parity distribution, and 20 cows in each group as experimental objects. From the first day to the seventh day, record the single yield and collect 3 milk samples every day, each of which is 50 ml. The sampling is required to be collected once in the morning, noon and evening, and mixed in a ratio of 4:3:3. Each milk sample is added with 6 parts per million benzoic acid as a preservative and stored at 4°C to form the sample set to be tested.

[0070] S102: The calcium content of the milk samples to be tested was tested, and the milk samples were graded based on the calcium concentration range of 120-130 mg / 100 ml as the standard for high-quality high-calcium milk. The experimental subjects in group C met the standard for high-quality high-calcium milk, and the four groups A, B, D, and E needed to supplement calcium. The details are shown in Table 1 below.

[0071] Table 1 Quality of milk in five groups

[0072] Milk yield / (L) Calcium concentration / (mg / 100ml) Milk grade Milk purchase price (yuan / L) A 45 109 qualified 2.6 B 44 118 medium 2.8 C 45 125 excellent 4.0 D 38 105 qualified 2.6 E 40 118 medium 2.8

[0073] S103: Calculate the apparent absorption rate of calcium by the experimental object and the total amount of calcium used for its own growth. The specific steps are as follows:

[0074] S301: Calculate the total calcium intake of the experimental subject during the feeding period W A From the first day to the seventh day, the total calcium content in the diet of the experimental subjects was measured every day, including roughage, concentrated feed, additives, water, etc., and the average value was taken. The formula is as follows:

[0075]

[0076] Where K i is the calcium concentration in the diet of experimental subject i, N iis the total weight of food consumed by experimental subject i during the feeding period, and n is the number of experimental subjects. The details are shown in Table 2 below.

[0077] Table 2 Daily calcium intake of experimental dairy cows

[0078] type Forage grass type Calcium content / (mg / kg) Feeding raw material mass / (kg) Total / (g) Summary / (g) Roughage Corn Silage 2500 15 37.5 122.61 straw 900 5 4.5 Concentrated feed corn 3000 4 12 Soybean meal 1540 3 4.62 barley 400 2 0.8 Brewer's grains 4000 2 8 Bran 16000 1 16 Premixes (with vitamins and minerals) ---- 0.5 0 additive Calcium Hydrogen Phosphate 331900 0.1 33.19 baking soda ---- 0.1 0 salt ---- 0.05 0 water 60 100 6

[0079] S302: Calculate the unused calcium content of the experimental subjects during the feeding cycle. From the first day to the seventh day, collect 2 kg of fresh manure from the experimental subjects' cowshed or sports field every day, mix and dry them for later use, store them in sampling bags at 4°C, and statistically evaluate the daily feces volume of the experimental subjects; similarly, collect urine every day, and statistically evaluate the daily urine volume of the experimental subjects. Detect the concentration of calcium in fecal and urine samples. The formula for calculating the unused calcium content of the experimental subjects during the feeding cycle is as follows:

[0080] in is the concentration of calcium in the feces of experimental subject i, W i is the total mass of feces of experimental subject i during the feeding period; is the concentration of calcium in the urine of experimental subject i, V i is the total mass of urine of experimental subject i during the feeding period; n is the number of experimental subjects; as shown in Table 3 below, it is found that the unutilized calcium element during the feeding period is 61.57 g.

[0081] Table 3 Data on apparent absorption rate of calcium in experimental dairy cows

[0082] Cow No. Total calcium content in cow dung (g / kg) Total amount of dry cow dung (kg) Calcium concentration in cow urine (g / L) Total amount of cow urine (L) Apparent absorption rate of calcium / (%) 1 9.00 6.20 0.24 27.66 49.08 2 8.81 6.11 0.25 25.18 50.96 3 8.70 6.34 0.27 25.02 49.50 4 9.22 6.00 0.23 26.31 49.95 5 9.28 5.89 0.25 25.18 50.29 6 9.19 5.93 0.26 25.18 50.21 7 8.93 6.31 0.23 26.05 49.16 8 8.84 6.05 0.29 24.11 50.68 9 8.97 5.96 0.27 24.98 50.90 10 8.88 6.30 0.24 25.57 49.37 11 8.77 6.07 0.27 24.98 51.08 12 8.73 6.46 0.25 23.52 49.21 13 8.81 6.10 0.25 25.41 50.99 14 8.81 6.36 0.24 25.49 49.31 16 8.89 6.34 0.27 24.78 48.57 17 9.11 6.27 0.23 26.83 48.38 18 9.19 6.07 0.26 25.18 49.16 19 9.19 6.07 0.27 24.76 49.05 20 9.19 6.07 0.27 24.55 49.10 average value 8.98 6.14 0.25 25.28 49.78

[0083] S303: Calculate the apparent absorption rate of calcium by the experimental subject, the formula is as follows:

[0084] in is the calculation result of step S301, The calculation result of step S302 is obtained by calculating the apparent absorption rate of calcium by the experimental subjects according to the experimental data in Table 1, Table 2 and Table 3. E 1 It is 49.78%.

[0085] S304: Calculate the calcium content in the milk sample produced by the experimental subject, the formula is as follows:

[0086] in C is the concentration of calcium in livestock product samples, Wis the mass of the livestock product sample. According to Table 1, it is calculated that W P It is 56.25 g.

[0087] S305: Calculate the amount of calcium required for the growth of the experimental object, the formula is as follows:

[0088] in E 1 is the calculation result of step S303, is the calculation result of step S301, C is the concentration of calcium in livestock product samples, W is the mass of the livestock product sample. W C =122.61*49.78%-56.25=4.78 g.

[0089] S104: Experimentally determine the calcium content of barley seeds after they are transformed into barley grass under clear water culture conditions. The specific steps are as follows:

[0090] S201: Select plump and healthy barley seeds, soak them in clean water for 15 hours, keep the water temperature at 25-28℃ and ventilate them to ensure that the seeds absorb enough water. After soaking, remove the seeds and control the water for 3 hours, then soak them again for 2-3 hours, keep the water temperature at 25-28℃ and ventilate them. After soaking, remove the seeds and lay them flat on the culture rack to keep the surface of the seeds moist.

[0091] S202: Use an automated control system to monitor parameters such as temperature, humidity, and light intensity. The temperature is controlled at 18-25°C and the humidity is 75%-95%. Cultivate in the dark for the first three days after sowing. On the fourth day, turn on the plant supplementary light for supplementary lighting. The light intensity is 2000-3000 lux to provide a good growth environment for barley grass until the seventh day.

[0092] S203: The whole plant of the barley grass grown to the seventh day is sampled, the sample weight is not less than 300g, and the calcium concentration is tested by near infrared detection. The details are shown in Table 4 below, and c1=0.83%.

[0093] S105: Experimentally determine the calcium enrichment capacity and maximum critical concentration of barley grass under nutrient solution culture conditions. The specific steps are as follows:

[0094] S401: Cultivating barley grass in a nutrient solution containing different concentrations of calcium, the cultivation method is the same as steps S201 and S202.

[0095] S402: Whole-plant sampling of barley grass grown to the seventh day after culture in nutrient solutions with different calcium concentrations was performed, with the sample weight not less than 300g, and calcium concentration testing was performed. Based on the growth trend and test results, the relationship between the calcium concentration in the nutrient solution and the calcium concentration in the barley grass (dry sample) was obtained. The linear regression was used to obtain , where x is the calcium concentration in the nutrient solution and y is the calcium concentration in barley grass (dry sample).

[0096] S403: Obtaining the inflection point value of the relationship between the calcium concentration in the nutrient solution and the calcium concentration in the barley grass (dry sample), which is the maximum critical concentration c of calcium that the barley grass can withstand. h As shown in Table 4 below, c h 350 mg / L, Growth inhibition occurred when the concentration exceeded 350 mg / L.

[0097] Table 4 Calcium content and seedling height of barley grass after culturing in nutrient solution with different calcium concentrations

[0098] deal with Calcium concentration in nutrient solution / (mg / L) Barley grass (dry sample) calcium concentration (%) Barley seedling height (cm) Shimizu 0 0.83 15 A 50 0.85 17 B 100 0.89 17.5 C 150 0.93 18 D 200 0.97 18.3 E 250 1 18.4 F 300 1.03 18.3 G 350 1.04 17.8 H 400 1.04 16.5 I 450 1.02 15 J 500 1.0 14 K 550 1.0 12 L 600 0.9 10 M 800 0.80 10

[0099] S404: Calculate the calcium enrichment capacity of barley grass, the formula is as follows:

[0100] Among them c is the cultured The calcium concentration of barley grass is poor. W D For fresh grass yield, W W is the water content of barley grass, △ C is the concentration difference of calcium in the nutrient solution, and V is the mass of the applied nutrient solution. It is calculated that when the calcium concentration in the nutrient solution is 350 mg / L, the average calcium enrichment capacity of barley grass is 58.33%.

[0101] S106: Determine the concentration range of calcium element according to the quality requirements of high-quality agricultural and livestock products. The specific steps are as follows:

[0102] S501: Using the milk product calcium content quality grading method, obtain the lower limit concentration of milk calcium content that meets the quality livestock product standards C D 120 mg / 100 ml ， Upper limit concentration C U 130 mg / 100 ml 。

[0103] S502: For the experimental subjects that do not meet the quality livestock product standards, calculate the lower limit of the calcium content in the barley grass nutrient solution required by them, the formula is as follows:

[0104] S503: For the experimental subjects that do not meet the quality livestock product standards, calculate the upper limit of the calcium content in the barley grass nutrient solution required by them, the formula is as follows:

[0105] in W C The total amount of calcium required for the growth of the test subjects to produce high-quality livestock products; W c The total amount of calcium required for the growth of experimental subjects that failed to produce high-quality livestock products; C D 、 C U is the result of step S501; C N Calcium concentration in products that do not meet quality livestock product standards; W For the production of high-quality livestock products, W N is the product output that does not meet the quality livestock product standard; c1 is the calcium concentration in barley grass under clear water culture conditions; a is the amount of barley grass fed in the diet; W W is the water content of barley grass; E 2 is the apparent absorption rate of calcium by the experimental subjects who failed to produce high-quality livestock products; v is the amount of nutrient solution poured each time; p is the frequency of nutrient solution pouring; and P is the enrichment capacity of barley grass for specific elements. a 106.77 mg / L, c b It is 764.66 mg / L.

[0106] S504: The concentration range of calcium in the barley grass nutrient solution is calculated to be the set With Collection The intersection of , where c h is the maximum critical concentration of calcium that barley grass can withstand. Since the critical concentration of calcium obtained in step S403 is 350 mg / L, the concentration range of calcium in the nutrient solution is .

[0107] S107 calculates the optimum concentration of the calcium element in the nutrient solution. The specific steps are as follows:

[0108] S601: Calculate the cost of the nutrient solution using the following formula:

[0109] in c is the concentration of calcium in the nutrient solution, v is the volume of the nutrient solution, u is the unit price of calcium raw material.

[0110] S602: Calculate the economic benefits of not adding nutrient solution, the formula is as follows:

[0111] in q 0 is the output of milk products when no nutrient solution is added, and u0 is the unit price of milk products when no nutrient solution is added. The calculated S0 is 56.8 yuan.

[0112] S603: Calculate the economic benefits of adding nutrient solution. The formula is as follows:

[0113] in q 1 The yield of milk products when adding nutrient solution, u 1 is the unit price of milk products when nutrient solution is added. S 2 It is 123.2 yuan.

[0114] S604: Calculate the incremental benefit of adding nutrient solution, the formula is as follows:

[0115] in S 0 For the economic benefits of not adding nutrient solution, S 2 To increase the economic benefits of adding nutrient solution, S 1 The cost of the nutrient solution.

[0116] S605: Through market research, the relationship between calcium concentration c and livestock product unit price u1 is obtained, which is expressed as follows: .

[0117] S606: Substitute the formula of S605 into the formula of S604 to obtain the relationship between the benefit and the concentration of calcium in the nutrient solution:

[0118] in , according to the result of step S504, .

[0119] S607: Calculate S in the interval The maximum value of S is the corresponding c, which is the concentration of calcium in the nutrient solution when the increase in milk product benefits after adding nutrient solution is the highest, that is, the optimal concentration. The maximum value is , , The maximum value of the three, where c z is the value of c when the derivative of S is 0. Further calculations yield is 52.026 yuan, is 47.49 yuan, It is 45.89 yuan, so the optimum concentration of calcium in the nutrient solution is 106.77 mg / L.

[0120] The calcium-containing nutrient solution prepared according to the above method is used to cultivate barley grass, and further fed to dairy cows, which can effectively increase the calcium content in dairy products and achieve quality improvement and efficiency enhancement of dairy products.

[0121] It should be noted that the above embodiments are only used to illustrate the technical method of the present invention and are not intended to limit it. Although it can be used as a reference, technicians can modify or replace the technical method of the invention with equivalents without departing from the scope of the technical method of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A method for preparing a nutrient solution of barley grass, the steps of the method are as follows: S101: construct an experimental object set of the pasture to be tested, randomly sample the livestock products of the experimental objects, register the sources of the samples, and form a sample set to be tested; S102: Detect the content of specific elements in the samples to be tested, and grade the quality of livestock products according to the element content indicators; S103: Calculate the apparent absorption rate of the experimental object to the specific element and the total amount of the specific element required for its own growth; S104: Experimental determination of the content of specific elements in barley seeds after conversion to barley grass under clear water culture conditions; S105: Experimental determination of the enrichment capacity and maximum critical concentration of specific elements of barley grass under nutrient solution culture conditions; S106: Calculate the concentration range of a specific element in the nutrient solution; S107: Calculate the optimum concentration of a specific element in the nutrient solution; Furthermore, the cultivation process of the barley grass and the determination of the content of specific elements are as follows: S201: Select plump and healthy barley seeds, soak them in clean water for 15 hours, keep the water temperature at 25-28℃ and ventilate them to ensure that the seeds fully absorb water. After soaking, remove the seeds and control the water for 3 hours, then soak them again for 2-3 hours, keep the water temperature at 25-28℃ and ventilate them. After soaking, remove the seeds and lay them flat on the culture rack to keep the surface of the seeds moist; S202: Use an automated control system to monitor parameters such as temperature, humidity, and light intensity. The temperature is controlled at 18-25°C and the humidity is 75%-95%. Cultivate in the dark for the first three days after sowing. On the fourth day, turn on the plant supplementary light for supplementary lighting. The light intensity is 2000-3000 lux to provide a good growth environment for barley grass until the seventh day. S203: Whole-plant sampling of barley grass on the seventh day of growth, with a sample weight of no less than 300g, is conducted for specific element concentration testing using near-infrared testing.

2. The method for preparing a nutrient solution of barley grass according to claim 1, characterized in that: The calculation process of the apparent absorption rate of the experimental object to a specific element and the element content used for its own growth in step S103 is as follows: S301: Calculate the intake of specific elements by the experimental subjects during the feeding period, the formula is as follows: Where K i is the specific element concentration in the diet of experimental subject i, N i is the total mass of food consumed by experimental subject i during the feeding period, and n is the number of experimental subjects; S302: Calculate the content of specific elements that are not utilized by the experimental object during the feeding cycle, the formula is as follows: in is the concentration of specific elements in the feces of experimental subject i, W i is the total mass of feces of experimental subject i during the feeding period; is the concentration of a specific element in the urine of subject i, V i is the total urine mass of experimental subject i during the feeding period; n is the number of experimental subjects; S303: Calculate the apparent absorption rate of the experimental object to a specific element, the formula is as follows: in is the calculation result of step S301, is the calculation result of step S302. So far, the apparent absorption rate of livestock to specific elements has been obtained; S304: Calculate the content of specific elements in the livestock product samples corresponding to the experimental object, the formula is as follows: Where C is the concentration of a specific element in the livestock product sample, and W is the mass of the livestock product sample; S305: Calculate the amount of specific elements required for the growth of the experimental object. The formula is as follows: 。 3. The method for preparing a nutrient solution of barley grass according to claim 1, characterized in that: The calculation process of the enrichment capacity and the maximum critical concentration of barley grass for specific elements under the nutrient solution culture conditions in step S105 is as follows: S401: Cultivating barley grass in nutrient solutions containing different concentrations of specific elements; S402: Whole-plant sampling of barley grass grown to the seventh day after culture in nutrient solutions with different concentrations of specific elements is performed, with the sample mass not less than 300g, and specific element concentration detection is performed. Based on the growth trend and the detection results, the relationship between the specific element concentration in the nutrient solution and the specific element concentration in the barley grass (dry sample) is obtained; S403: Obtaining the inflection point value of the relationship between the concentration of the specific element in the nutrient solution and the concentration of the specific element in the barley grass (dry sample), which is the maximum critical concentration c of the specific element that the barley grass can withstand h ; S404: Calculate the enrichment capacity of barley grass for specific elements. The formula is as follows: Where △c is the concentration difference of specific elements in the cultivated barley grass, W D is the fresh grass yield, W W is the water content of barley grass, △C is the concentration difference of specific elements in the nutrient solution, and V is the mass of the applied nutrient solution.

4. The method for preparing a nutrient solution of barley grass according to claim 1, characterized in that: The calculation process of the concentration range of the specific element in the nutrient solution in step S106 is as follows: S501: According to the quality grading method of trace element content in livestock products, the lower limit concentration of trace element content that meets the quality livestock product standards is calculated as C D , the upper limit concentration is C U ; S502: For the experimental subjects that do not meet the quality livestock product standards, calculate the lower limit of the content of specific trace elements in the barley grass nutrient solution required by them, the formula is as follows: S503: For the experimental subjects that do not meet the quality livestock product standards, calculate the upper limit of the content of specific trace elements in the barley grass nutrient solution required by them, the formula is as follows: Where W C The total amount of specific elements required for the growth of experimental subjects to produce high-quality livestock products; W c The total amount of specific elements required for the growth of experimental subjects that failed to produce high-quality livestock products; C D , C U is the result of step S501; C N The concentration of specific elements in products that do not meet the quality livestock product standards; W is the output of high-quality livestock products, W N is the product output that does not meet the quality livestock product standards; c1 is the specific element concentration after barley seeds are converted into barley grass under clear water culture conditions; a is the amount of barley grass fed in the diet; W W is the water content of barley grass; E2 is the apparent absorption rate of specific elements by the experimental subjects that failed to produce high-quality livestock products; v is the amount of nutrient solution poured each time; p is the frequency of nutrient solution pouring; P is the enrichment capacity of barley grass for specific elements; S504: The concentration range of specific elements in the barley grass nutrient solution is calculated as a set c a c b With Collection 0 c h The intersection of c a c b ∩ 0 c h , where c h It is the maximum critical concentration of a specific element that barley grass can withstand.

5. The method for preparing a nutrient solution of barley grass according to claim 1, characterized in that: The calculation process of the optimum concentration of a specific element in the nutrient solution in step S107 is as follows: S601: Calculate the cost of the nutrient solution using the following formula: Where c is the concentration of a specific element in the nutrient solution, v is the volume of the nutrient solution, and u is the unit price of the specific element raw material; S602: Calculate the economic benefits of not adding nutrient solution, the formula is as follows: Where q0 is the output of livestock products when no nutrient solution is added, The unit price of livestock products when no nutrient solution is added; S603: Calculate the economic benefits of adding nutrient solution. The formula is as follows: Among them, q1 is the output of livestock products when the nutrient solution is added, and u1 is the unit price of livestock products when the nutrient solution is added; S604 calculates the incremental benefit of adding nutrient solution, and the formula is as follows: Where S0 is the economic benefit without adding nutrient solution, S2 is the economic benefit with adding nutrient solution, and S1 is the cost of nutrient solution; S605: Through market research, the relationship between the concentration of specific elements c and the unit price of livestock products u1 is obtained, which is expressed as follows: ; S606: Substitute the formula of S605 into the formula of S604 to obtain the relationship between the benefit and the element concentration in the nutrient solution: in c ∈ c a c b ∩ 0 c h ; S607: Calculate S in the interval c a c b ∩ 0 c h The maximum value of c is the concentration of specific elements in the nutrient solution corresponding to the highest increase in livestock product benefits from adding nutrient solution, that is, the optimal concentration.

6. The method for preparing a nutrient solution of barley grass according to claim 3, characterized in that: In the step S401, the nutrient solution is used to culture barley grass. The nutrient solution can be applied by adding the nutrient solution to a water storage device for hydroponics, mist culture, soil culture, or substrate culture. The nutrient solution can also be added to a pulp raw material to make culture paper, and the paper containing the nutrient solution is laid on a culture vessel before culture. The nutrient solution can also be made into a seed coating.

7. The method for preparing a nutrient solution of barley grass according to claim 5, characterized in that: The specific element may be one of the trace elements required for livestock growth, such as calcium, iron, zinc, and selenium.

8. The method for preparing a nutrient solution of barley grass according to claim 5, characterized in that: The livestock product may be any one of meat, eggs and milk.

9. The method for preparing a nutrient solution of barley grass according to claim 5, characterized in that: The step S107 can not only analyze a single specific element to obtain the optimal concentration for configuring the nutrient solution, but also analyze multiple specific elements. Further, for the analysis of multiple specific elements, one method is to perform the calculation of step S107 on each of the multiple elements, and then configure each element into a mixed nutrient solution according to its own optimal concentration; another method is to expand the formula in step S601 to , expand the formula in step S605 to ,in is the vector of the concentration of each element in the nutrient solution, is the unit price vector of each element raw material, and then the calculation of steps S606 and S607 is performed to obtain The optimal value of The optimal configuration is a mixed nutrient solution.

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