Method for reducing ash content of pleurotus eryngii mushroom bran feed

Abstract

The invention belongs to the technical field of edible mushroom bran feed, and particularly relates to a method for reducing the ash content of pleurotus eryngii mushroom bran feed, which comprises the following steps: S1, preparing a mushroom bran fermentation base material; s2, activating the fermentation inoculant; s3, mixed fermentation: inoculating the activated fermentation inoculant into the mushroom bran fermentation base material, uniformly mixing, filling into a breathing fermentation bag, compacting, exhausting air in the bag, sealing, and fermenting at room temperature for 45 days to obtain the pleurotus eryngii mushroom bran fermentation feed. The pleurotus eryngii mushroom bran feed is fermented by adopting a mixed microbial agent of bacillus subtilis, candida utilis, lactobacillus plantarum and aspergillus niger, the change condition of the ash content ratio in the fermented pleurotus eryngii mushroom bran is studied, and it is found that the ash content in the pleurotus eryngii mushroom bran feed can be remarkably reduced; edible mushroom bran can be reasonably and fully utilized as an unconventional feed resource, and the method has important significance in solving the problem of feed shortage in the livestock and poultry breeding process, promoting healthy development of the breeding industry, saving resources and protecting the environment.

Description

Technical Field

[0001] The invention belongs to the technical field of edible mushroom bran feed, and particularly relates to a method for reducing ash content in pleurotus eryngii mushroom bran feed. Background Art

[0002] The edible fungi industry in my country has developed rapidly, and the output of edible fungi by-products is huge. According to statistics, every 1kg of edible fungi produced will produce 3.5-5.0kg of fungus bran. At present, the main ways to use edible fungus bran are to cultivate edible fungi, substrate organic fertilizer, feed and extract active substances. Pleurotus eryngii bran is the remaining part of the cultivation substrate after the fruiting body is harvested. It generally contains fibrous materials such as sawdust, crop straw, corn cobs, cottonseed hulls, and auxiliary materials such as rice bran, bran, and soybean meal. After harvesting, the bran still retains a large amount of mycelium and various metabolites, which is extremely nutritious. Reasonable and full use of edible fungus bran as an unconventional feed resource can solve the problem of feed shortage in livestock and poultry breeding, which has a huge driving effect on the development of the breeding industry, while also saving resources and protecting the environment.

[0003] For herbivorous animals such as cattle and sheep, the ash content in their food is naturally high, and the ash content in their feed may also be slightly higher, but too high an ash content will reduce the nutritional value of the feed and may have adverse effects on the health of the animals. Therefore, reasonable control of the ash content in the feed is one of the important measures to ensure the quality of the feed.

[0004] The inventors used a mixed bacterial agent of Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger to ferment the Pleurotus eryngii bran feed, and studied the changes in the ash content in the Pleurotus eryngii bran after fermentation. It was found that the ash content in the Pleurotus eryngii bran feed can be significantly reduced, which is conducive to the rational and full use of edible bran as an unconventional feed resource, and is of great significance for solving the problem of feed shortage in livestock and poultry breeding, promoting the healthy development of the breeding industry, saving resources and protecting the environment.

[0005] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0006] The object of the present invention is to provide a method for reducing the ash content of Pleurotus eryngii bran feed.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A method for reducing the ash content of Pleurotus eryngii bran feed comprises the following steps:

[0009] 1. A method for reducing the ash content of Pleurotus eryngii bran feed, characterized in that it comprises the following steps:

[0010] S1. Prepare the fermentation base of fungus husk

[0011] S2. Activated fermentation agent

[0012] The fermentation agent formed by mixing Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger is activated with molasses water;

[0013] S3. Mixed fermentation

[0014] The activated fermentation agent is inoculated into the fermentation base material of the mushroom residue, mixed evenly and put into the breathing fermentation bag, compacted to remove the air in the bag and sealed, and fermented at room temperature for 45 days to obtain the fermented feed of the mushroom residue.

[0015] Preferably, in S1, the fermented mushroom bran base is mixed with the following ingredients, measured in parts by weight: 80-100 parts of Pleurotus eryngii mushroom bran, 0-20 parts of sugarcane leaves and 0-10 parts of cassava residues; wherein the base of the Pleurotus eryngii mushroom bran is: sugarcane bagasse, corn cobs, corn flour, bran and light calcium.

[0016] Preferably, in S2, the mass ratio of Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger is 4:3:3:10.

[0017] Preferably, in S2, the Brix of the molasses water is 16° Bx.

[0018] Preferably, in S3, the amount of activated fermentation bacteria added is 5%.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The invention adopts a mixed bacterial agent of Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger to ferment Pleurotus eryngii bran feed, studies the change of ash proportion in Pleurotus eryngii bran after fermentation, and finds that the ash content in Pleurotus eryngii bran feed can be significantly reduced, which is beneficial to the rational and full use of edible bran as an unconventional feed resource, and is of great significance to solving the problem of feed shortage in livestock and poultry breeding, promoting the healthy development of breeding industry, saving resources and protecting the environment. DETAILED DESCRIPTION

[0021] The following is a clear and complete description of the technical solution of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Materials and Methods

[0023] 1. Main Materials and Reagents

[0024] Pleurotus eryngii bran: provided by the edible fungus factory of the Institute of Microbiology, Guangxi Academy of Agricultural Sciences. Its base materials are sugarcane bagasse, corn cobs, corn flour, bran and light calcium.

[0025] Molasses: purchased from Mingyang Sugar Factory of Guangxi Nanning Sugar Company, with an initial Brix of 78°Bx, and diluted with water to a suitable Brix.

[0026] Bacillus subtilis (CICC10732), Candida utilis (CICC32722), Lactobacillus plantarum (CICC22703) and Aspergillus niger (CICC40273) were purchased from China Industrial Culture Collection Center (CICC).

[0027] Fermentation agent: Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger were prepared into A1-A4 fermentation agents in different proportions (see Table 1). Then, appropriate amount of molasses water (brix 16°Bx) was taken according to the weight of the raw materials, and the fermentation agents were added respectively and stirred evenly, and then placed for activation for 30 minutes.

[0028] Table 1 Fermentation agent combination

[0029]

[0030] Mushroom residue fermentation base: Pleurotus eryngii mushroom residue, sugarcane leaves and cassava residue were respectively free of impurities and crushed through a 40-mesh sieve, and then formed into B1-B6 raw material combinations (Table 2) in different proportions. Before fermentation, random samples were taken to determine the ash content. Three parallel samples were used and the average value of the parallel samples was taken. The results are shown in Table 3.

[0031] Table 2 Bacteria chaff fermentation base

[0032]

[0033] Table 3 Ash content of fungus bran fermentation base material before fermentation

[0034]

[0035] 2. Methods

[0036] The activated fermentation agent was inoculated into the fermentation base material with an inoculation rate of 5% to form the T1-T24 treatment groups, which were mixed evenly and put into the breathing fermentation bag, compacted to remove the air in the bag and sealed, and fermented at room temperature for 45 days to obtain the fermented feed of Pleurotus eryngii. After the fermentation was completed, the bag was opened and randomly sampled to determine the ash content after fermentation. Three parallel samples were used and the average value of the parallel samples was taken. The results are shown in Table 4.

[0037] Table 4 Treatment combinations of Pleurotus eryngii bran feed and ash content after fermentation

[0038]

[0039]

[0040] It can be seen from Table 4 that when different raw material combinations of Pleurotus eryngii bran are fermented with different strain ratios, when the raw material combination is B1 and B2, fermentation with different bacterial agents will increase the Ash content of the product; when the raw material combination is B3, B4, and B5, fermentation with A4 bacterial agent can reduce the Ash content, while fermentation with other bacterial agents will significantly increase it.

[0041] After 45 days of fermentation with A4, the ash content of B3 combination decreased from 9.48% to 8.81%, a decrease of 7.1%; the ash content of B4 combination decreased from 11.13% to 8.14%, a decrease of 26.9%; the ash content of B5 combination decreased from 10.35% to 8.55%, a decrease of 17.4%. Among the various combinations of strains, the content of Aspergillus niger in A4 was higher, and Aspergillus niger can decompose large molecular organic matter and insoluble inorganic matter, which can promote the absorption and utilization of nutrients.

[0042] In summary, the present invention uses the A4 bacterial agent with a relatively high proportion of Aspergillus niger to ferment the feed raw material mainly composed of Pleurotus eryngii bran, which has a significant effect on reducing the ash content of Pleurotus eryngii bran feed. The ash content of B3, B4, and B5 combinations was significantly reduced after fermentation, among which the ash content of the B4 combination was reduced from 11.13% to 8.14%, a decrease of 26.9%. It can be seen that the present invention uses a mixed bacterial agent of Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger to ferment Pleurotus eryngii bran feed, and studies the change in the proportion of ash in Pleurotus eryngii bran after fermentation. It is found that it can significantly reduce the ash content in Pleurotus eryngii bran feed, which is conducive to the rational and full use of edible bran as an unconventional feed resource, and is of great significance to solving the problem of feed shortage in livestock and poultry breeding, promoting the healthy development of the breeding industry, saving resources, and protecting the environment.

[0043] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A method for reducing the ash content of Pleurotus eryngii bran feed, characterized in that: The following steps are involved: S1. Prepare the fermentation base of fungus husk S2. Activated fermentation agent The fermentation agent formed by mixing Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger is activated with molasses water; S3. Mixed fermentation The activated fermentation agent is inoculated into the fermentation base material of the mushroom residue, mixed evenly and put into the breathing fermentation bag, compacted to remove the air in the bag and sealed, and fermented at room temperature for 45 days to obtain the fermented feed of the mushroom residue.

2. The method for reducing ash content of Pleurotus eryngii bran feed according to claim 1, characterized in that: In S1, the fermented mushroom bran base material is mixed with the following ingredients, measured in parts by weight: 80-100 parts of Pleurotus eryngii mushroom bran, 0-20 parts of sugarcane leaves and 0-10 parts of cassava residues; wherein the base material of the Pleurotus eryngii mushroom bran is: sugarcane residue, corn cobs, corn flour, bran and light calcium.

3. The method for reducing ash content of Pleurotus eryngii bran feed according to claim 1, characterized in that: In S2, the mass ratio of Bacillus subtilis, Candida utilis, Lactobacillus plantarum and Aspergillus niger is 4:3:3:

10.

4. The method for reducing ash content of Pleurotus eryngii bran feed according to claim 1, characterized in that: In S2, the Brix of molasses water is 16°Bx.

5. The method for reducing ash content of Pleurotus eryngii bran feed according to claim 1, characterized in that: In S3, the amount of activated fermentation bacteria added is 5%.

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