Nucleic acid extraction device capable of preventing cross contamination

Abstract

The invention belongs to the technical field of nucleic acid detection equipment, and particularly relates to a nucleic acid extraction device capable of preventing cross contamination. A plurality of first bases are distributed on the front side of the transmission belt in the left-right direction, a plurality of second bases are distributed in the middle of the transmission belt in the left-right direction, and a plurality of third bases are distributed on the rear side of the transmission belt in the left-right direction; the first base, the second base and the third base are all used for mounting extraction pipes; the blocking mechanism is arranged at the upper end in the extraction cavity and used for separating every two adjacent extraction pipes in the left-right direction. The first motor is used for driving the synchronous wheel to rotate, the synchronous belt can be driven for transmission, so that the extraction pipe is driven to change the position in the extraction cavity, and the synchronous belt is used for transmission, so that the extraction pipe can be finely adjusted, and the extraction pipe can correspond to the stirring rod conveniently. By arranging the blocking mechanism, the two adjacent extraction pipes can be positioned, so that the extraction pipes further accurately correspond to the stirring rods.

Description

Technical Field

[0001] The invention belongs to the technical field of nucleic acid detection equipment, and in particular relates to a nucleic acid extraction device that prevents cross contamination. Background Art

[0002] With the continuous development of medical technology, there have been great breakthroughs in nucleic acid extraction technology. Nucleic acid extractors are instruments that use matching nucleic acid extraction reagents to automatically complete sample nucleic acid extraction. They are widely used in disease control centers, clinical disease diagnosis, environmental microbial testing, food safety testing, animal husbandry, molecular biology research and other fields.

[0003] However, when performing nucleic acid extraction, the stirring rod needs to be inserted into different extraction tubes, and the extraction tubes are often installed on an extraction carrier, and the extraction carrier is driven by a driving device such as a hydraulic cylinder to move back and forth in the extraction chamber so that the extraction tube corresponds to the stirring rod. Due to the error of the movement, it is sometimes difficult to drive the extraction tube to accurately correspond to the stirring rod. Therefore, it is necessary to design a nucleic acid extraction device that prevents cross contamination to solve the above problem. Summary of the invention

[0004] In view of the above problems, the present invention provides a cross-contamination-proof nucleic acid extraction device to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: a nucleic acid extraction device for preventing cross contamination, comprising an extraction chamber, a synchronous wheel, a transmission belt, a first motor and a blocking mechanism;

[0006] The plurality of synchronous wheels are rotatably connected to the lower end of the extraction chamber, the transmission belt is sleeved on the plurality of synchronous wheels, a plurality of first bases are distributed along the left-right direction on the front side of the transmission belt, a plurality of second bases are distributed along the left-right direction on the middle part of the transmission belt, and a plurality of third bases are distributed along the left-right direction on the rear side of the transmission belt, and the first base, the second base and the third base are all used to install the extraction tube;

[0007] The blocking mechanism is arranged at the upper end of the extraction chamber and is used to separate two adjacent extraction tubes in the left and right directions.

[0008] Furthermore, a synchronous wheel is distributed at each of the four corners of the lower end of the extraction chamber, a synchronous wheel is arranged in the middle of the right side of the lower end of the extraction chamber, and the transmission belt is sleeved on the five synchronous wheels, and the transmission belt has a double S-shaped structure.

[0009] Furthermore, the cross-contamination-proof nucleic acid extraction device also includes a bottom plate and a support plate, both of which are located at the lower end of the extraction chamber, the support plate is located below the bottom plate, the synchronous wheel is rotatably connected to the bottom plate, and the first motor is installed on the support plate.

[0010] Furthermore, the cross-contamination-proof nucleic acid extraction device also includes a fan, a connecting pipe and a filter. Vents are provided on the first base, the second base and the third base. The connecting pipe is located below the first base, the second base and the third base. The fan is connected to the inside of the extraction chamber, and the vent is connected to the filter through the connecting pipe.

[0011] Furthermore, the cross-contamination-proof nucleic acid extraction device also includes a second motor, a first rack and a first gear, the first rack is vertically arranged and connected to the connecting pipe, the second motor is connected to the extraction chamber and is transmission-connected to the first gear, and the first gear is meshed with the first rack.

[0012] Furthermore, the barrier mechanism includes a driving mechanism, a top plate and a barrier member, the driving mechanism is located above the first base, the second base and the third base, the lower end of the driving mechanism is transmission-connected to the top plate, a plurality of barrier members are distributed along the left-right direction at the lower end of the top plate, a row of the first base, the second base and the third base in the front-to-back direction are located in a barrier member, and the driving mechanism is used to drive the barrier member to move in the vertical direction.

[0013] Furthermore, the barrier member includes two partitions, and a row of the first base, the second base, and the third base in the front-to-back direction is located between the two partitions.

[0014] Furthermore, the driving mechanism also includes a connecting bar, a third motor, a second rack and a second gear. The four second racks are located at the four corners of the upper end of the top plate and are vertically arranged. The two connecting bars are respectively located at the front and rear ends of the extraction chamber. The left and right ends of the connecting bar are connected to the third motor. The four third motors are respectively connected to the four second gears, and the four second gears are respectively meshed with the four second racks.

[0015] Technical effects and advantages of the present invention:

[0016] 1. The first motor is used to drive the synchronous wheel to rotate, which can drive the synchronous belt transmission, and drive the first base, the second base and the third base to change their positions inside the extraction chamber, thereby driving the front row, the middle row and the back row of extraction tubes to change their positions inside the extraction chamber. The use of synchronous belt transmission is helpful to fine-tune the position of the extraction tube, making it easier to match the extraction tube to the stirring rod, thereby facilitating the stirring rod to be inserted into different extraction tubes respectively.

[0017] 2. By setting up a blocking mechanism, two adjacent extraction tubes can be positioned so that the extraction tubes can further accurately correspond to the stirring rods; secondly, it can also prevent aerosols containing nucleic acid molecules from entering adjacent extraction tubes, which is conducive to obtaining uncontaminated nucleic acid extracts, thereby ensuring the accuracy of nucleic acid test results.

[0018] Other features and advantages of the present invention will be described in the following description, and partly become obvious from the description, or be understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures pointed out in the description and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 A schematic structural diagram of a nucleic acid extraction device for preventing cross contamination according to an embodiment of the present invention is shown;

[0021] Figure 2 A schematic diagram showing the external structure of the extraction chamber according to an embodiment of the present invention is shown;

[0022] Figure 3 A schematic diagram showing the internal structure of the extraction chamber according to an embodiment of the present invention is shown;

[0023] Figure 4 A schematic structural diagram of a transmission mechanism according to an embodiment of the present invention is shown;

[0024] Figure 5 A structural schematic diagram of the transmission mechanism of an embodiment of the present invention from another perspective is shown.

[0025] Figure numerals: 1. extraction chamber; 21. synchronous wheel; 22. transmission belt; 221. first base; 222. second base; 223. third base; 23. bottom plate; 24. support plate; 25. support frame; 26. vent; 3. first motor; 411. connecting strip; 412. third motor; 413. second rack; 414. second gear; 42. top plate; 421. interface; 43. barrier; 51. fan; 52. connecting pipe; 53. filter; 54. second motor; 55. first rack; 56. first gear; 6. stirring rod. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] like Figures 1 to 5 As shown, a cross-contamination-proof nucleic acid extraction device according to an embodiment of the present invention comprises an extraction chamber 1, a synchronous wheel 21, a transmission belt 22, a first motor 3 and a blocking mechanism;

[0028] The plurality of synchronous wheels 21 are rotatably connected to the lower end of the extraction chamber 1, the transmission belt 22 is sleeved on the plurality of synchronous wheels 21, a plurality of first bases 221 are distributed along the left-right direction on the front side of the transmission belt 22, a plurality of second bases 222 are distributed along the left-right direction on the middle part of the transmission belt 22, and a plurality of third bases 223 are distributed along the left-right direction on the rear side of the transmission belt 22, and the first base 221, the second base 222 and the third base 223 are all used to install the extraction tube;

[0029] The blocking mechanism is arranged at the upper end of the extraction chamber 1 and is used to separate two adjacent extraction tubes in the left-right direction.

[0030] Specifically, a tube seat is provided on the first base 221, the second base 222 and the third base 223, and the extraction tube can be inserted into the tube seat. Secondly, the axial direction of the synchronous wheel 21 is oriented in the vertical direction.

[0031] In this embodiment, a plurality of first bases 221 are arranged in the left-right direction of the front side of the transmission belt 22, a plurality of second bases 222 are arranged in the left-right direction of the middle part of the transmission belt 22, and a plurality of third bases 223 are arranged in the left-right direction of the transmission belt 22. For example, a first extraction tube can be arranged on the first base 221, a second extraction tube can be arranged on the second base 222, and a third extraction tube can be arranged on the third base 223, that is, a plurality of first extraction tubes are located at the front side of the extraction chamber 1, a plurality of second extraction tubes are located at the middle part of the extraction chamber 1, and a plurality of third extraction tubes are located at the rear side of the extraction chamber 1.

[0032] The first motor 3 drives the transmission belt 22 to drive the first base 221, the second base 222 and the third base 223 on the transmission belt 22 to change their positions. That is, it can be adjusted so that a plurality of second bases 222 and the second extraction tubes are located at the front side of the extraction chamber 1, a plurality of third bases 223 and the third extraction tubes are located at the middle of the extraction chamber 1, and a plurality of first bases 221 and the first extraction tubes are located at the rear side of the extraction chamber 1; it can also be adjusted so that a plurality of third bases 223 and the third extraction tubes are located at the front side of the extraction chamber 1, a plurality of first bases 221 and the first extraction tubes are located at the middle of the extraction chamber 1, and a plurality of second bases 222 and the second extraction tubes are located at the rear side of the extraction chamber 1.

[0033] Among them, the first extraction tube is an extraction tube with a magnetic rod sleeve, the second extraction tube is an extraction tube with a washing solution, and the third extraction tube is an extraction tube with an elution solution. The stirring rod 6 is inserted into the first extraction tube to extract the solution, and the stirring rod 6 with the magnetic rod sleeve stirs the mixed solution in the second extraction tube to complete the nucleic acid lysis of the sample and the binding of the nucleic acid and the magnetic beads; the stirring rod 6 with the magnetic rod sleeve stirs and mixes the mixed solution in the third extraction tube, and washes the magnetic beads adsorbed with nucleic acid, so as to obtain a contamination-free nucleic acid extract, thereby ensuring the accuracy of the nucleic acid detection results.

[0034] Secondly, by setting a blocking mechanism at the upper end of the extraction chamber 1, the blocking mechanism can be used to isolate two adjacent first extraction tubes, two adjacent second extraction tubes and two adjacent third extraction tubes, so as to prevent the aerosol containing nucleic acid molecules in the first extraction tube, the second extraction tube and the third extraction tube from entering the adjacent first extraction tube, the second extraction tube and the third extraction tube.

[0035] Therefore, the first motor 3 is used to drive the synchronous wheel 21 to rotate, which can drive the synchronous belt transmission, and drive the first base 221, the second base 222 and the third base 223 to change their positions inside the extraction chamber 1, thereby driving the front row, middle row and rear row of extraction tubes to change their positions in the front-to-back direction and the left-to-right direction inside the extraction chamber 1. The use of synchronous belt transmission is helpful to fine-tune the position of the extraction tube in the left-to-right direction, making it easier to match the extraction tube to the stirring rod 6, thereby facilitating the stirring rod 6 to be inserted into different extraction tubes respectively.

[0036] Secondly, by setting up a blocking mechanism, two adjacent extraction tubes can be positioned so that the extraction tubes can further accurately correspond to the stirring rod 6; secondly, it can also prevent aerosols containing nucleic acid molecules from entering adjacent extraction tubes, which is conducive to obtaining uncontaminated nucleic acid extracts, thereby ensuring the accuracy of nucleic acid detection results.

[0037] Alternatively, if Figure 4 As shown, a synchronous wheel 21 is distributed at each of the four corners of the lower end of the extraction chamber 1, a synchronous wheel 21 is arranged in the middle of the right side of the lower end of the extraction chamber 1, and the transmission belt 22 is sleeved on the five synchronous wheels 21, and the transmission belt 22 is a double S-shaped structure.

[0038] In this embodiment, by setting the transmission belt 22 as a double S-shaped structure, the transmission belt 22 can be divided into a front side, a middle side and a rear side. By setting the first base 221, the second base 222 and the third base 223 on the transmission belt 22, the first base 221, the second base 222 and the third base 223 can be easily distributed in the front and rear directions.

[0039] Secondly, during the transmission process of the transmission belt 22, the first base 221 is located at the front side of the extraction chamber 1, the second base 222 is located in the middle of the extraction chamber 1, and the third base 223 is located at the rear side of the extraction chamber 1. The first base 221 first moves to the rear side of the extraction chamber 1 with the transmission belt 22, the second base 222 moves to the front side of the extraction chamber 1 with the transmission belt 22, and the third base 223 moves to the middle of the extraction chamber 1 with the transmission belt 22, and then moves in sequence. The first base 221 can be provided with a first extraction tube, a second extraction tube or a third extraction tube, for example, the first base 221 is provided with a second extraction tube, the third base 223 is provided with a first extraction tube, and the second base 222 is provided with a third extraction tube, that is, the first extraction tube, the second extraction tube and the third extraction tube do not need to be placed in the extraction chamber 1 in order. When performing nucleic acid extraction, it is only necessary to drive through the transmission belt 22 to move different extraction tubes to the bottom of the stirring rod 6, so as to facilitate the rapid placement of the first extraction tube, the second extraction tube and the third extraction tube.

[0040] Alternatively, if Figure 4 and Figure 5 As shown, the cross-contamination-proof nucleic acid extraction device also includes a bottom plate 23 and a support plate 24, both of which are located at the lower end of the extraction chamber 1, and the support plate 24 is located below the bottom plate 23, the synchronous wheel 21 is rotatably connected to the bottom plate 23, and the first motor 3 is installed on the support plate 24.

[0041] Specifically, Figure 4 and Figure 5As shown, the cross-contamination-preventing nucleic acid extraction device further includes a support frame 25 , through which the bottom plate 23 and the support plate 24 are connected.

[0042] In this embodiment, the first motor 3 is used to drive a synchronous wheel 21 to rotate, and multiple synchronous wheels 21 are sleeved with synchronous belts, which can drive multiple synchronous wheels 21 to rotate and drive the synchronous belt transmission. Secondly, by providing a bottom plate 23, it is convenient to connect multiple synchronous wheels 21 to the bottom plate 23; and by installing a support plate 24 under the bottom plate 23, the first motor 3 can be installed on the support plate 24.

[0043] Alternatively, if Figure 1 As shown, the cross-contamination-proof nucleic acid extraction device also includes a fan 51, a connecting pipe 52 and a filter 53. Vent holes 26 are provided on the first base 221, the second base 222 and the third base 223. The connecting pipe 52 is located below the first base 221, the second base 222 and the third base 223. The fan 51 is connected to the inside of the extraction chamber 1, and the vent hole 26 is connected to the filter 53 through the connecting pipe 52.

[0044] Specifically, an interface is opened at the upper end of the connecting pipe 52, and a plurality of interfaces are distributed along the left-right direction, and the interfaces are connected with the vent holes 26 correspondingly.

[0045] In this embodiment, a vent hole 26 is provided on the first base 221, the second base 222 and the third base 223, and a connecting pipe 52 is provided in the middle of the transmission belt 22. When the first base 221, the second base 222 or the third base 223 moves to the top of the connecting pipe 52, the fan 51 is used to blow air into the extraction chamber 1, so that the aerosol in the first extraction tube, the second extraction tube or the third extraction tube can enter the connecting pipe 52 through the vent hole 26, and be discharged after being filtered by the filter 53 and meeting the exhaust standard.

[0046] Alternatively, if Figure 4 and Figure 5 As shown, the cross-contamination-proof nucleic acid extraction device also includes a second motor 54, a first rack 55 and a first gear 56. The first rack 55 is vertically arranged and connected to the connecting tube 52. The second motor 54 is connected to the extraction chamber 1 and is transmission-connected to the first gear 56. The first gear 56 is meshed with the first rack 55.

[0047] In this embodiment, the first gear 56 is driven to rotate by the second motor 54, and the first gear 56 is engaged with the first rack 55 to drive the first rack 55 to move in the vertical direction. The first rack 55 is connected to the connecting tube 52, so that the connecting tube 52 moves in the vertical direction, so that the docking port on the connecting tube 52 can be tightly attached to and docked with the vent holes 26 on the first base 221, the second base 222 and the third base 223, thereby preventing aerosol from leaking from the gap between the connecting tube 52 and the first base 221, the second base 222 or the third base 223.

[0048] Alternatively, if Figure 2 and Figure 3 As shown, the barrier mechanism includes a driving mechanism, a top plate 42 and a barrier member 43. The driving mechanism is located above the first base 221, the second base 222 and the third base 223. The lower end of the driving mechanism is transmission-connected to the top plate 42. A plurality of barrier members 43 are distributed along the left-right direction at the lower end of the top plate 42. A row of the first base 221, the second base 222 and the third base 223 in the front-to-back direction are located in a barrier member 43. The driving mechanism is used to drive the barrier member 43 to move in the vertical direction.

[0049] Specifically, in the front-to-back direction, a row of first bases 221, second bases 222 and third bases 223 form a group and are arranged in a barrier member 43. Secondly, an extraction port 421 is provided on the top plate 42, and a plurality of extraction ports 421 are sequentially distributed along the left-right direction, and the plurality of extraction ports 421 correspond to the middle of the transmission belt 22. By providing a plurality of extraction ports 421 on the top plate 42, the stirring rod 6 can be easily passed through the top plate 42 and extended into the first extraction tube, the second extraction tube and the third extraction tube.

[0050] In this embodiment, a driving mechanism is used to drive the barrier 43 to move in the vertical direction. When the first base 221, the second base 222 and the third base 223 remain stable, the driving mechanism is used to drive the barrier 43 to move downward, so that the barrier 43 blocks two adjacent first extraction tubes, two adjacent second extraction tubes and two adjacent third extraction tubes to avoid cross contamination.

[0051] Secondly, when the first base 221, the second base 222 and the third base 223 are adjusted in position, the driving mechanism is used to drive the barrier 43 to move upward, so that the barrier 43 moves to the top of the first extraction tube, the second extraction tube and the third extraction tube, so as to prevent the barrier 43 from interfering with the movement of the first extraction tube, the second extraction tube and the third extraction tube.

[0052] Optionally, the barrier member 43 includes two partitions, and a row of the first base 221 , the second base 222 , and the third base 223 in the front-to-back direction are located between the two partitions.

[0053] In this embodiment, by setting the barrier 43 as two partitions, each two partitions can correspond to a group of first base 221, second base 222 and third base 223, so that the barrier 43 is used to isolate two adjacent first extraction tubes, second extraction tubes and third extraction tubes.

[0054] Alternatively, if Figure 3 As shown, the driving mechanism also includes a connecting bar 411, a third motor 412, a second rack 413 and a second gear 414. The four second racks 413 are located at the four corners of the upper end of the top plate 42 and are vertically arranged. The two connecting bars 411 are respectively located at the front and rear ends of the extraction chamber 1. The left and right ends of the connecting bar 411 are connected to the third motor 412. The four third motors 412 are respectively connected to the four second gears 414, and the four second gears 414 are respectively meshed with the four second racks 413.

[0055] In this embodiment, the second motor 54 drives the second gear 414 to rotate, and the second gear 414 engages with the second rack 413 to drive the second rack 413 to move, thereby driving the top plate 42 to move in the vertical direction, thereby driving the barrier 43 to move in the vertical direction, thereby ensuring the effect of the driving mechanism.

[0056] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nucleic acid extraction device for preventing cross contamination, characterized in that: It comprises an extraction chamber (1), a synchronous wheel (21), a transmission belt (22), a first motor (3) and a blocking mechanism; The plurality of synchronous wheels (21) are rotatably connected to the lower end of the extraction chamber (1); the transmission belt (22) is sleeved on the plurality of synchronous wheels (21); a plurality of first bases (221) are distributed along the left-right direction on the front side of the transmission belt (22); a plurality of second bases (222) are distributed along the left-right direction on the middle part of the transmission belt (22); a plurality of third bases (223) are distributed along the left-right direction on the rear side of the transmission belt (22); the first base (221), the second base (222) and the third base (223) are all used for installing the extraction tube; The blocking mechanism is arranged at the upper end of the extraction chamber (1) and is used to separate two adjacent extraction tubes in the left-right direction.

2. The cross-contamination-proof nucleic acid extraction device according to claim 1, characterized in that: A synchronous wheel (21) is distributed at each of the four corners of the lower end of the extraction chamber (1), a synchronous wheel (21) is arranged in the middle of the right side of the lower end of the extraction chamber (1), and the transmission belt (22) is sleeved on the five synchronous wheels (21), and the transmission belt (22) is in a double S-shaped structure.

3. The cross-contamination-proof nucleic acid extraction device according to claim 2, characterized in that: The invention also comprises a bottom plate (23) and a support plate (24), wherein the bottom plate (23) and the support plate (24) are both located at the lower end of the extraction chamber (1), the support plate (24) is located below the bottom plate (23), the synchronous wheel (21) is rotatably connected to the bottom plate (23), and the first motor (3) is mounted on the support plate (24).

4. The cross-contamination-proof nucleic acid extraction device according to claim 3, characterized in that: The invention also comprises a fan (51), a connecting pipe (52) and a filter (53); the first base (221), the second base (222) and the third base (223) are all provided with ventilation holes (26); the connecting pipe (52) is located below the first base (221), the second base (222) and the third base (223); the fan (51) is connected to the inside of the extraction chamber (1); and the ventilation hole (26) is connected to the filter (53) through the connecting pipe (52).

5. The cross-contamination-proof nucleic acid extraction device according to claim 4, characterized in that: The invention also comprises a second motor (54), a first rack (55) and a first gear (56), wherein the first rack (55) is vertically arranged and connected to the connecting pipe (52), the second motor (54) is connected to the extraction chamber (1) and is transmission-connected to the first gear (56), and the first gear (56) is meshed with the first rack (55).

6. The cross-contamination-proof nucleic acid extraction device according to claim 1, characterized in that: The barrier mechanism comprises a driving mechanism, a top plate (42) and a barrier member (43); the driving mechanism is located above the first base (221), the second base (222) and the third base (223); the lower end of the driving mechanism is drivingly connected to the top plate (42); a plurality of barrier members (43) are distributed along the left-right direction at the lower end of the top plate (42); a row of the first base (221), the second base (222) and the third base (223) in the front-back direction are located in one barrier member (43); and the driving mechanism is used to drive the barrier member (43) to move along the vertical direction.

7. The cross-contamination-proof nucleic acid extraction device according to claim 6, characterized in that: The barrier member (43) comprises two partitions, and a row of the first base (221), the second base (222) and the third base (223) in the front-to-back direction is located between the two partitions.

8. The cross-contamination-proof nucleic acid extraction device according to claim 7, characterized in that: The driving mechanism also includes a connecting bar (411), a third motor (412), a second rack (413) and a second gear (414); the four second racks (413) are located at the four corners of the upper end of the top plate (42) and are vertically arranged; the two connecting bars (411) are respectively located at the front and rear ends of the extraction chamber (1); the left and right ends of the connecting bar (411) are both connected to the third motor (412); the four third motors (412) are respectively connected to the four second gears (414); the four second gears (414) are respectively meshed with the four second racks (413).

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