Emulsifier-producing strain field aerobic rapid fermentation device and method

Abstract

The invention discloses an on-site aerobic rapid fermentation device and method for an emulsifier-producing strain, and the method comprises the following steps: arranging an activated carbon canister and an immersed ultrafiltration system on an oil field site, and carrying out continuous treatment on oil field produced water subjected to water quality analysis to obtain fermentation and injection water; therefore, aerobic fermentation can be quickly completed after emulsifier-producing bacteria seeds are inoculated into a fermentation incubator, and the obtained fermentation liquor has the characteristics of stable quality and excellent oil displacement performance as a microbial agent, and an effective way is provided for reducing the microbial oil recovery cost.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial oil recovery and relates to the development and application of an on-site aerobic rapid fermentation process of emulsifier-producing bacteria. Background Art

[0002] Microbial oil recovery technology is to improve reservoir conditions to promote the role of oil recovery functional bacteria in the reservoir environment, including using the growth and metabolism of oil recovery functional bacteria to promote crude oil emulsification, improve crude oil fluidity, and reduce crude oil viscosity, thereby achieving an increase in crude oil recovery. Compared with other oil recovery technologies, microbial oil recovery technology is simple to operate and has low cost. It can be applied to different reservoir environments. At the same time, it will not produce environmentally harmful substances during oil field application, making it more environmentally friendly.

[0003] The oil recovery functional bacteria contained in the reservoir involve dozens to hundreds of endogenous microbial species, among which hydrocarbon oxidizing bacteria that can grow and metabolize with crude oil as a carbon source and produce a large amount of biological emulsifiers are the most important type of oil recovery bacteria with emulsification function. The on-site injection process of endogenous microbial oil recovery activator usually uses the superior oil recovery functional bacteria selected from the on-site produced fluid, and uses them to purchase the fermented corresponding bacterial agents or emulsifier extracts (the bacterial agents also contain bacterial components relative to the emulsifier extracts, and the oil recovery effect is better) after fermentation in the factory, and then transport them to the site and inject them together after compounding with the activator. Although the factory can use strictly aseptic production equipment (which objectively leads to high production costs), due to the long distance between the factory and the oil field site, the transportation device of the bacterial agent cannot be guaranteed to be free of contamination during transportation, and the injection also takes a certain amount of time (it cannot be guaranteed to be injected on site in time). Therefore, especially in summer, the long delay between the factory and the site makes it easy for the bacterial agent to deteriorate before injection, thus affecting the on-site use effect.

[0004] In order to ensure the quality of the microbial agent and the realization of the injection process of large-scale and low cost on site, Chinese patent CN116144470A proposed a fermentation process of oil recovery functional bacteria suitable for use on site in oil fields, but the fermentation temperature of the strain in the patent is high, the process is also relatively complicated (for example, the culture medium prepared with oil field injection water needs to be heated and sterilized), and the oil recovery performance of the microbial agent obtained by fermentation is insufficient (for example, the crude oil recovery rate obtained by physical modeling experiments without dilution of the fermentation liquid is less than 10%). Chinese patents CN111394280A and CN116285930A proposed a culture medium formula that can promote the growth of oil recovery functional bacteria and the generation of metabolites, but did not consider the low utilization efficiency of organic nitrogen sources by the oil recovery functional bacteria in the microbial agent in the injection environment. In addition, for most of the post-treatment processes of oil field produced water, the goal is to obtain softened water (combined use of activated carbon adsorption, ultrafiltration, and nanofiltration), and there is no treatment process design and feasibility evaluation related to oil recovery functional bacteria fermentation and reinjection water. Summary of the invention

[0005] In view of the problems and shortcomings of the prior art, the present invention provides an on-site aerobic rapid fermentation device and method for emulsifier-producing bacteria.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] An on-site aerobic rapid fermentation device for producing emulsifier bacteria comprises a water treatment component and a fermentation component arranged at an oil field site, wherein the water treatment component comprises an activated carbon adsorption device and an ultrafiltration device, and the fermentation component comprises a fermentation culture box using oil well injection water as fermentation water, wherein the oil well injection water is obtained by treating oil field produced water in sequence through the activated carbon adsorption device and the ultrafiltration device.

[0008] Preferably, the water treatment component specifically includes the following four parts:

[0009] ①Raw water tank: used to store produced water from oil fields, thus providing water source for subsequent water treatment, and can also be used to collect water quality test samples before water treatment;

[0010] ② Activated carbon tank: used for activated carbon adsorption treatment of oil field produced water injected into it (into the activated carbon tank) through the raw water tank. Activated carbon has a large specific surface area, developed micropores, high mechanical strength, and fast adsorption speed. It has a high removal rate for various impurities in oil field produced water, such as organic impurities, demulsifiers, flocculants, fungicides and other harmful substances;

[0011] ③ Immersed ultrafiltration system: used to filter the oilfield produced water injected into the activated carbon tank (i.e. the oilfield produced water injected into the immersed ultrafiltration system after activated carbon adsorption) with an ultrafiltration membrane with a rated pore size range of 0.3-0.03μm. By utilizing the selective permeability of the ultrafiltration membrane, suspended matter, microorganisms and some macromolecular organic matter in the water body can be retained in the immersed ultrafiltration system, thereby achieving the effect of purifying the water body;

[0012] ④ Water production tank: used to store oil well injection water (i.e. oil field produced water after activated carbon adsorption and ultrafiltration membrane filtration), so as to provide water source for fermentation and bacterial agent injection (specifically used for the preparation of fermentation culture medium, transfer of seed liquid, and dilution of bacterial agent obtained by fermentation), and can also be used for the collection of water quality test samples after water treatment.

[0013] Preferably, the ambient temperature at the oil field site is 0-40°C (a suitable ambient temperature to ensure the effective operation of the device), and the reservoir temperature is 25-50°C.

[0014] Preferably, the raw water tank, activated carbon tank, submerged ultrafiltration system and produced water tank are all arranged in a constant temperature environment, so as to make full use of the thermal energy of the oil well injected water (the oil field produced fluid becomes oil field produced water after oil-water separation, and the temperature is generally between 20-40°C) for subsequent fermentation, which is beneficial to reduce the power consumption during fermentation.

[0015] Preferably, the fermentation component also includes a culture medium stock liquid tank, which is respectively connected to the water production tank and the fermentation culture box, so that a part of the oil well injection water in the water production tank can be used to make the fermentation culture medium therein (in the culture medium stock liquid tank) into a stock liquid and then injected into the fermentation culture box.

[0016] Preferably, the fermentation component also includes a seed liquid culture box connected to the fermentation culture box. The seed liquid culture box can be used to store seed liquid prepared in advance and transported to the oil field site. The seed liquid culture box can also be used to culture emulsifier-producing bacteria and store the seed liquid obtained by culture; wherein the seed liquid is injected into the fermentation culture box before fermentation.

[0017] Preferably, the culture medium stock liquid tank, fermentation incubator, and seed liquid incubator are all arranged in a constant temperature environment, thereby reducing additional heating power consumption during fermentation and facilitating maintaining a stable fermentation environment.

[0018] An on-site aerobic rapid fermentation method for emulsifier-producing bacteria, the on-site aerobic rapid fermentation method comprising the following steps:

[0019] (1) Fermentation water treatment stage

[0020] At the oil field site, the produced water is subjected to activated carbon adsorption and ultrafiltration in sequence to obtain oil well injection water;

[0021] (2) Fermentation stage

[0022] At the oil field site, the emulsifier-producing bacteria seeds are inoculated into a culture medium with the above-mentioned oil well injection water as the solvent, and then cultured. At the end of the culture, the culture solution (or bacterial solution) obtained is the bacterial agent for injection at the oil field site, wherein the culture temperature is 30-40°C, the culture time is ≥18 hours, and the dissolved oxygen in the culture system is 4-10 mg / L.

[0023] Preferably, step 1 specifically includes the following steps: when the oilfield produced water is qualified through water quality analysis, it can directly enter the above-mentioned fermentation culture box and be used as water for bacterial fermentation and culture; when it is unqualified, the oilfield produced water is sequentially treated by the above-mentioned activated carbon tank and the immersion ultrafiltration system and then enters the above-mentioned fermentation culture box through the above-mentioned water production tank after it is qualified.

[0024] Preferably, the emulsifier-producing bacteria are selected from one or more hydrocarbon-oxidizing bacteria of the genera Pseudomonas and Rhodococcus.

[0025] Preferably, in step 2, the culture medium used by the emulsifier-producing bacteria (specifically, hydrocarbon-oxidizing bacteria of the genus Rhodococcus) comprises: 0.5-2 g / L molasses, 0.5-1 g / L ammonium sulfate, 0.05-0.5 g / L magnesium sulfate, and ≤2 g / L yeast powder.

[0026] Preferably, in step 2, the components of the culture medium used by the emulsifier-producing bacteria (specifically, Pseudomonas hydrocarbon-oxidizing bacteria) include: 10-40 g / L glycerol, 2-5 g / L sodium nitrate, 0.5-2 g / L sodium chloride, 0.5-2 g / L ammonium chloride, ≤5 g / L potassium dihydrogen phosphate, ≤5 g / L sodium hydrogen phosphate, and ≤0.5 g / L magnesium sulfate.

[0027] Preferably, in step 2, the pH of the culture system is 5.5-7.2 (depending only on the change of pH during the culture process).

[0028] Preferably, in step 2, the inoculation amount of the emulsifier-producing bacteria seeds is determined in the following manner: the bacterial concentration is ≥10 8 The seed solution of pcs / mL is inoculated into the culture medium at a ratio of 5%-10%.

[0029] Preferably, the step 2 specifically comprises the following steps:

[0030] 2.1 The emulsifier-producing bacteria seeds (seed liquid) cultured in the factory or laboratory are transported to the oil field site and stored in the above-mentioned seed liquid culture box;

[0031] 2.2 Use the matching lifting pump to add the emulsifier-producing bacteria seeds (seed liquid) from the seed liquid culture box to the above-mentioned fermentation culture box, and use the matching dosing pump to add the culture medium stock solution in the above-mentioned culture medium stock liquid tank to the fermentation culture box, and use the oil well injection water in the above-mentioned water production tank to dilute it to within 80% of the effective volume of the fermentation culture box (the total volume of the culture system), and then under ventilation (specifically turning on the air compressor to supplement oxygen) and stirring (using a stirrer to stir continuously) conditions, constant temperature culture for 18-64 hours to obtain the bacterial solution, and then use the fermentation box discharge pump to inject the bacterial solution in the fermentation culture box as a bacterial agent into the liquid preparation tank (used for dilution and compounding before on-site injection of the bacterial agent).

[0032] Preferably, the seed liquid culture box is also equipped with a steam generator. After the seed liquid culture box is emptied, the steam generator can be used to steam sterilize the seed liquid culture box, and after the temperature is lowered, it can continue to be used for culturing and / or storing seed liquid.

[0033] Preferably, the fermentation culture boxes are equipped with 2-3, and each fermentation culture box can be steam sterilized by using a matching steam generator after being emptied, and can continue to be used for fermentation after being cooled down (i.e., emulsifier-producing bacteria seeds are connected for cultivation); this sterilization step is repeated alternately in different fermentation culture boxes.

[0034] Preferably, the preparation of the culture medium stock solution specifically includes the following steps: adding the culture medium raw materials (specifically refer to the components of the above-mentioned culture medium) and a portion of the oil well injection water in the above-mentioned water production tank into the culture medium stock solution tank, and then stirring and mixing them with a stirrer. After the culture medium raw materials are completely dissolved, they can be added to the fermentation culture box using a dosing pump.

[0035] The above-mentioned on-site aerobic rapid fermentation method of emulsifier-producing bacteria is applied in microbial oil recovery.

[0036] Preferably, the dilution ratio of the bacterial agent is 1:9-49 (ie, the dilution multiple is 10-50 times, and the corresponding concentration after dilution is 10%-2%), and the diluent can be the oil well injection water in the above-mentioned water production tank.

[0037] Preferably, under the initial formation conditions of microbial oil recovery, due to the oligotrophic nature of the formation and the low bacterial concentration in the formation water, when the dilution ratio is increased (for example, the dilution is more than 50 times), an activator containing a nitrogen source, a carbon source, and a phosphorus source can be compounded (that is, injected simultaneously with the diluted bacterial agent) to ensure the growth, reproduction, and metabolism of the oil recovery functional bacteria (for example, the above-mentioned emulsifier-producing bacteria species); after a period of injection and when a biological field of a certain concentration has been formed in the formation, the activator can be reduced or no longer compounded when the bacterial agent is injected.

[0038] The beneficial effects of the present invention are embodied in:

[0039] The present invention proposes a process device and a corresponding method for utilizing oilfield produced water to realize fermentation and cultivation of emulsifier-producing bacteria on-site in the oilfield. Specifically, activated carbon adsorption and ultrafiltration are used as means for fermentation and injection water treatment, and the bacteria fermentation and cultivation process can achieve adaptive domestication of the reinjection environment with the help of the treated oilfield produced water, thereby obtaining a microbial agent with stable quality (less contamination by foreign bacteria) on-site in the oilfield through simple and rapid aerobic fermentation, and achieving the purpose of low-cost and high-efficiency oil recovery after dilution and reinjection.

[0040] Furthermore, the process technology of the present invention adopts filtration and steam sterilization in the whole process, which greatly reduces the contamination of miscellaneous bacteria. At the same time, the culture medium does not need to be sterilized separately, which reduces power consumption and simplifies the process.

[0041] Furthermore, the present invention targets specific species of hydrocarbon oxidizing bacteria (Pseudomonas and Rhodococcus), not only providing the fermentation water, temperature, and oxygen required for proliferation, but also optimizing the required nutrients (culture medium components). It can rapidly increase the bacterial biomass of the culture system through fermentation culture while promoting the production of emulsifier products, thereby obtaining a bacterial agent for on-site injection in oil fields with excellent oil recovery performance (experimental results show that the crude oil recovery rate can still be increased by more than 10% when diluted 50 times). BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is the ground on-site fermentation process equipment and flow chart.

[0043] Figure 2 This is a schematic diagram of the process layout of the fermentation water treatment stage (the outermost frame shows that the raw water tank, activated carbon adsorption equipment, ultrafiltration equipment, production water tank, etc. are located in the same constant temperature board room. Considering that the oil field site is far away from the urban area, especially the desert has a large temperature difference between day and night and the pipe network is prone to freezing in winter, the indoor temperature is guaranteed to be greater than 25°C).

[0044] Figure 3 Schematic diagram of the process layout for the fermentation and culture stage (the outermost frame indicates that the fermentation culture box, culture medium stock liquid tank, seed liquid culture box, etc. are located in the same constant temperature room, ensuring that the indoor temperature is 25-40℃).

[0045] Figure 4 This is the H3 fermentation curve (the product is glycoprotein).

[0046] Figure 5 This is the DB fermentation curve (the product is glycolipid). DETAILED DESCRIPTION

[0047] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. The embodiments are only used to explain the technical solution of the present invention, but not to limit the protection scope of the present invention.

[0048] 1. Source of strains

[0049] Strains with good crude oil emulsification effect were screened out from the produced water of block A in Xinjiang Oilfield, and the strains H3 belonging to Rhodococcus were identified; strain DB belonging to Pseudomonas screened out from other blocks was also used.

[0050] 2. Select the experimental oil field site

[0051] In the above Block A, the reservoir temperature is 37°C, the pressure is 10 MPa, and the permeability is 1800 mD (i.e. 1800×10 -3 μm 2 ), geological reserves of 142×10 4 t.

[0052] 3. On-site rapid fermentation process of emulsifier-producing bacteria

[0053] Example 1

[0054] This example refers to Figure 1 The process shown in the figure was used to carry out the fermentation experiment of strain H3 in block A (the specific layout of the experimental device is as follows Figure 2 , Figure 3 The specific steps are as follows:

[0055] (1) Fermentation water treatment stage

[0056] After oil-water separation (gravity separation, air flotation, and sedimentation to remove oil and scale) of the produced liquid from Block A, 10 cubic meters of produced water were stored in a raw water tank (2.4×2×2.5m). Through water quality analysis, it was found that the TGB content in the produced water was 1.2×10 5 / mL, which does not meet the water quality standards for fermentation water. 3 / h, N = 0.75kw) is injected into the activated carbon tank (Φ1.4m×4m), and then injected into the submerged ultrafiltration system for adsorption filtration. The ultrafiltration effluent (filtered water) is injected into the water production tank (2.4×2×2.5m) for storage (water is filtered through Figure 2 The water was then pumped out using the external discharge pump shown in the figure) and the water quality was analyzed. The following Tables 1 and 2 show the analysis results before and after treatment.

[0057] Table 1. Comparison of produced water treatment effects before and after treatment (microorganisms)

[0058]

[0059] Note: HOB stands for hydrocarbon oxidizing bacteria

[0060] Table 2. Comparison of produced water effects before and after treatment (physical and chemical)

[0061]

[0062] It can be seen from Tables 1 and 2 that in order to ensure the fermentation effect of the strains and reduce the contamination of miscellaneous bacteria, the fermentation water derived from oilfield produced water needs to be pre-treated.

[0063] For the above activated carbon tank, considering the adsorption performance and service life of activated carbon, economical and practical coal-based granular activated carbon is used as the adsorbent. 3 / h, N=5.5kw) to backwash the activated carbon tank to ensure the filtering effect.

[0064] The above-mentioned submerged ultrafiltration system mainly includes a membrane module (the rated pore size of the ultrafiltration membrane is 0.03 μm), a membrane flushing fan, a membrane outlet pump, a membrane backwash pump, a membrane backwash dosing device, a membrane backwash dosing pump, and a safety filter; the submerged ultrafiltration membrane can be regularly cleaned using a membrane backwash dosing device (V=100L, N=0.37kw) and a membrane backwash dosing pump (Q=150L / h, H=3.0bar, N=0.37kw) to ensure the filtering effect.

[0065] (2) Fermentation stage

[0066] The emulsifier-producing strains (specifically H3 seed liquid) cultured in the factory or laboratory are transported to the site and stored in a seed liquid culture box. Each time the seed liquid culture box is emptied, steam sterilization is performed using a steam generator (200kg / h, N=7.5kw). After cooling, the emulsifier-producing strains can continue to be stored. The culture medium raw materials and water in the water production tank are added to the culture medium stock liquid tank, and stirred using a stirrer to obtain a culture medium raw material solution.

[0067] Use lift pump (Q = 1m 3 / h, N = 0.37kw) to produce emulsifier bacteria about 500L (seed liquid bacterial concentration of 10 8 / mL) was added from the seed liquid culture box to the fermentation culture box (2.4×2×2.5m), and the culture medium raw material solution (i.e., stock solution) in the culture medium stock tank was added to the fermentation culture box by using the dosing pump, and water was added from the water production tank to dilute it to 80% of the effective volume (12 cubic meters) of the fermentation culture box (the culture medium composition is: molasses 1g / L, ammonium sulfate 0.5g / L, magnesium sulfate 0.05g / L, and yeast powder 0.5g / L; inoculation ratio 5%), and the air compressor (Q = 0.2m 3 / min, P = 24.5kpa, N = 0.37kw; that is Figure 3 The fermentation liquid was supplemented with oxygen by a blower and stirred continuously with a stirrer to ensure that the oxygen content in the fermentation liquid was controlled at 4 mg / L (the ventilation volume was maintained at 2-4 L / min) required by the functional strain H3. The temperature in the fermentation incubator was maintained at 37°C for 36 hours. At this time, the fermentation liquid monitoring bacterial concentration reached 8.65×10 8 / mL (similar to and slightly higher than the bacterial concentration after 18 hours of culture), and the emulsifier production was 2.34g / L (see Figure 4 ), the proportion of miscellaneous bacteria is less than 10%, and can be injected into the subsequent dilution and compounding links using the fermentation box discharge pump.

[0068] The above-mentioned fermentation culture boxes are equipped with two, each fermentation culture box is emptied once, steam sterilized by using a steam generator, and can continue to be used for fermentation after cooling down, and the other fermentation culture box repeats this step (that is, the two fermentation culture boxes are used in turn and sterilized before use). When the fermentation culture box is used to culture the strain, the water in the water production tank is used to prepare the culture medium in the fermentation culture box and inoculate it, and the matching electric heating equipment is used to maintain a certain water temperature in the fermentation culture box (that is, a small amount of heating is required to reach the adaptation temperature of the strain), so as to meet the fermentation needs of the strain.

[0069] Example 2

[0070] This example refers to Figure 1 The process shown was carried out in block A with strain DB fermentation process experiment.

[0071] (1) Fermentation water treatment stage

[0072] Same as Example 1.

[0073] (2) Fermentation stage

[0074] The emulsifier-producing strains (specifically DB seed liquid) cultured in the factory or laboratory are transported to the site and stored in a seed liquid culture box. Each time the seed liquid culture box is emptied, steam sterilization is performed using a steam generator (200kg / h, N=7.5kw). After cooling, the emulsifier-producing strains can continue to be stored. The culture medium raw materials and water in the water production tank are added to the culture medium stock liquid tank, and stirred using a stirrer to obtain a culture medium raw material solution.

[0075] Use lift pump (Q = 1m 3 / h, N = 0.37kw) to produce emulsifier bacteria about 500L (seed liquid bacterial concentration of 10 8 / mL) was added from the seed liquid culture box to the fermentation culture box (2.4×2×2.5m), and the culture medium raw material solution (i.e., stock solution) in the culture medium stock tank was added to the fermentation culture box by using the dosing pump, and water was added from the water production tank to dilute it to 80% of the effective volume (12 cubic meters) of the fermentation culture box (the culture medium composition is: glycerol 40g / L, sodium nitrate 4.5g / L, sodium chloride 1g / L, ammonium chloride 1g / L, potassium dihydrogen phosphate 3.5g / L, sodium hydrogen phosphate 3.5g / L, and magnesium sulfate 0.3g / L; inoculation ratio 5%), and the air compressor (Q=0.2m 3 / min, P = 24.5kpa, N = 0.37kw) to supplement oxygen, and continuously use a stirrer to ensure that the oxygen content in the fermentation liquid is controlled at 8-9mg / L (the ventilation volume is maintained at 1-2L / min) required by the functional strain DB, and the temperature in the fermentation incubator (37°C) is maintained for 20 hours. At this time, the fermentation liquid monitoring bacterial concentration reaches 8.41×10 8 / mL, and continued fermentation for 64 hours. At this time, the emulsifier production of the fermentation broth was 8.6g / L (see Figure 5 ).

[0076] from Figure 5It can be seen from the DB fermentation curve shown that when the fermentation time is short, the growth of the emulsifier concentration in the fermentation liquid lags behind the bacterial concentration. Therefore, when continuous injection is required on site, 2-3 fermentation culture boxes are required to meet different fermentation process requirements. For example, 2 fermentation culture boxes are equipped when pursuing bacterial concentration, and 3 fermentation culture boxes are equipped when pursuing emulsifier production. Each fermentation culture box is emptied once (for example, the fermentation box discharge pump is used to inject into the subsequent dilution and compounding links), and steam sterilized using a steam generator. After cooling, it can continue to be used for fermentation. Repeat this step for other fermentation culture boxes (that is, 2 or 3 fermentation culture boxes are used in turn and sterilized before use).

[0077] In the above two examples, the biological indicators of the water in the water production tank meet the injection requirements, so it can be used to dilute the fermentation liquid (respectively called H3 bacterial liquid and DB bacterial liquid), solving the problem that most of the current oil field injection water is reinjection wastewater (that is, the produced water formed after the oil-water separation of the above-mentioned produced liquid), which causes damage to the bacterial reinjection environment.

[0078] 4. Application of Microbial Agent Reinjection

[0079] The H3 bacterial solution (fermentation 48h) and DB bacterial solution (fermentation 48h) obtained by fermentation were used and diluted in different proportions (1:9, 1:49) to simulate the reinjection of microbial agents through experiments (see Table 3).

[0080] Table 3. Microbial flooding (7 days) experimental conditions and results

[0081]

[0082] Note: Recovery rate increase (%) = microbial flooding final recovery rate (%) - water flooding recovery rate (%)

[0083] The results show that when using H3 bacterial solution for oil recovery experiments and injecting 1PV into artificial cemented cores, the recovery rate can be increased by more than 11% (compared with the water recovery rate); when using DB bacterial solution for oil recovery experiments and injecting 1PV into artificial cemented cores, the recovery rate can be increased by more than 13% (compared with the water recovery rate).

[0084] 5. Process Application Cost Accounting

[0085] Calculated based on the H3 bacterial agent obtained by fermentation, after applying the above example process:

[0086] (1) The main energy consumption is electricity cost of about 14.2 yuan / cubic liquid; the reagent cost is 19.68 yuan / cubic liquid; the main consumables are membrane components and activated carbon. The membrane components are replaced every 3 years, and the investment per ton of water is about 2.4 yuan / cubic liquid. The activated carbon is replaced about once a year, and the investment per ton of water is about 9.85 yuan / cubic liquid. The total consumables cost is about 12.25 yuan / cubic liquid. The total fermentation operation cost is 46.13 yuan / ton. According to the diluted concentration of 2% (diluted 50 times) injected into the oil field, the total operation cost is 0.9 yuan / cubic liquid.

[0087] (2) Equipment investment: RMB 1.26 million + relocation fee: RMB 70,000 + construction fee: RMB 70,000 = RMB 1.4 million. Based on the annual injection volume of 128,000 cubic meters on site, the total equipment cost is RMB 10.9 per cubic meter of liquid.

[0088] (3) The factory purchased seed liquid at RMB 8,000 per cubic meter, inoculated it with 5% of the H3 strain, and injected it at a diluted concentration of 2% after fermentation, with a total seed cost of RMB 8 per cubic meter;

[0089] (4) The investment for the analysis and testing of the supporting bacterial concentration and emulsifier production is RMB 500,000. Based on the injection of 128,000 cubic meters, the total testing cost is RMB 3.9 per cubic meter of liquid;

[0090] The above four items add up to 23.7 yuan / cubic meter of liquid for injection into the well site. The original microbial agent liquid is directly purchased from the factory at 8,000 yuan / cubic meter, and diluted (50 times) at a volume ratio of 1:49 before injection into the well site. The cost of the liquid injected into the well site is 160 yuan / cubic meter. Therefore, the process cost of the present invention is reduced by 85%.

[0091] In summary, the present invention aims at the problems of using reinjected sewage as injection water in the process of microbial oil recovery, high cost of purchasing microbial agents from factories, and insufficient oil recovery performance of microbial agents. Based on the principles of advanced technology, safety, reliability, and economic rationality and feasibility, the present invention draws on and refers to advanced domestic and foreign technologies and experiences, insists on starting from reality, takes into account reducing power consumption and saving the use of agents, and proposes an on-site aerobic rapid fermentation process for emulsifier-producing bacteria with simple process and stable fermentation effect. It can not only reduce the application cost of bacteria for oil recovery, but also ensure the quality and effect of microbial agents injected for microbial oil recovery under the condition of real-time production of microbial agents and injection water on site, thus achieving the best technical and economic results.

Claims

1. An on-site aerobic rapid fermentation device for producing emulsifier bacteria, characterized by: The on-site aerobic rapid fermentation device comprises a water treatment component and a fermentation component arranged at the oil field site, wherein the water treatment component comprises an activated carbon adsorption device and an ultrafiltration device, and the fermentation component comprises a fermentation culture box which uses oil well injection water as fermentation water, wherein the oil well injection water is obtained by treating oil field produced water through the activated carbon adsorption device and the ultrafiltration device.

2. According to claim 1, an on-site aerobic rapid fermentation device for producing emulsifier bacteria, characterized in that: The water treatment component specifically includes an activated carbon tank, an immersed ultrafiltration system, a raw water tank for storing oil field produced water and a production water tank for storing oil well injected water. The raw water tank, activated carbon tank, immersed ultrafiltration system and the production water tank are connected in sequence.

3. The on-site aerobic rapid fermentation device for producing emulsifier bacteria according to claim 2, characterized in that: The fermentation component also includes a culture medium stock liquid tank, which is connected to the water production tank and the fermentation culture tank respectively.

4. The on-site aerobic rapid fermentation device for producing emulsifier bacteria according to claim 1, characterized in that: The fermentation component also includes a seed liquid culture box for culturing and / or storing emulsifier-producing bacteria seeds, and the seed liquid culture box is connected to the fermentation culture box, wherein the emulsifier-producing bacteria are selected from one or more of Pseudomonas and Rhodococcus.

5. A method for rapid on-site aerobic fermentation of emulsifier-producing bacteria, characterized in that: The on-site aerobic rapid fermentation method comprises the following steps: 1) At the oil field site, the produced water is subjected to activated carbon adsorption and ultrafiltration in sequence to obtain oil well injection water; 2) Inoculate the emulsifier-producing bacteria seeds into a culture medium with oil well injection water as the solvent at the oil field site, and then culture it. The culture solution obtained at the end of the culture is the bacterial agent for injection at the oil field site, wherein the culture temperature is 30-40°C, the culture time is ≥18 hours, and the dissolved oxygen in the culture system is 4-10 mg / L.

6. The method for rapid on-site aerobic fermentation of emulsifier-producing bacteria according to claim 5, characterized in that: The emulsifier-producing bacteria are selected from one or more of Pseudomonas and Rhodococcus.

7. The method for rapid on-site aerobic fermentation of emulsifier-producing bacteria according to claim 6, characterized in that: The culture medium used by the emulsifier-producing bacteria of the genus Rhodococcus comprises: 0.5-2 g / L molasses, 0.5-1 g / L ammonium sulfate, 0.05-0.5 g / L magnesium sulfate, and ≤2 g / L yeast powder.

8. The method for rapid on-site aerobic fermentation of emulsifier-producing bacteria according to claim 6, characterized in that: The components of the culture medium used by the emulsifier-producing bacteria of the genus Pseudomonas include: 10-40 g / L glycerol, 2-5 g / L sodium nitrate, 0.5-2 g / L sodium chloride, 0.5-2 g / L ammonium chloride, ≤5 g / L potassium dihydrogen phosphate, ≤5 g / L disodium hydrogen phosphate, and ≤0.5 g / L magnesium sulfate.

9. The method for rapid on-site aerobic fermentation of emulsifier-producing bacteria according to claim 5, characterized in that: The inoculation amount of the emulsifier-producing bacteria seeds is determined in the following manner: the bacterial concentration is ≥10 8 The seed solution of pcs / mL is inoculated into the culture medium at a ratio of 5%-10%.

10. An application of the on-site aerobic rapid fermentation method of emulsifier-producing strains as claimed in any one of claims 5 to 9 in microbial oil recovery, characterized in that: The dilution ratio of the bacterial agent is 1:9-49, and the diluent is oil well injection water.

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