Chinese sturgeon health level evaluation system based on blood indexes

Abstract

The invention relates to a Chinese sturgeon health level evaluation system based on a blood index, and the system comprises a blood sample processing module which is used for processing a blood sample of Chinese sturgeons to obtain a serum sample of the Chinese sturgeons; the determination module is used for determining the serum sample of the Chinese sturgeon by adopting a full-automatic biochemical analyzer to obtain a blood sugar observation record, a blood fat observation record, a serum protein observation record, a metabolite observation record and a serum biochemical enzyme observation record; the selection module is used for selecting a blood index from the blood sugar observation record, the blood fat observation record, the serum protein observation record, the metabolite observation record and the serum biochemical enzyme observation record; the scoring module is used for scoring the selected blood indexes; and the evaluation module evaluates the health level of the Chinese sturgeons according to the scores of the blood indexes. The health condition of the Chinese sturgeons can be accurately evaluated.

Description

Technical Field

[0001] The invention relates to the technical field of fishery resource conservation, and in particular to a Chinese sturgeon health level assessment system based on blood indicators. Background Art

[0002] The Chinese sturgeon (Acipenser sinensis) is a first-class protected aquatic wild animal in my country, belonging to the order Acipenseriformes, family Acipenseridae, genus Acipenser. In 1988, the Chinese sturgeon was listed as a first-class protected aquatic wild animal in my country, and several nature reserves were established in the Yangtze River Basin. my country's scientific research institutions are committed to the protection of the Chinese sturgeon. In 2009, they broke through the difficulty of artificial breeding, providing technical support for the recovery of the Chinese sturgeon population. At the same time, artificial propagation and release and rescue measures are used to promote the improvement of the quantity and quality of the Chinese sturgeon population. However, how to accurately assess the health status of the Chinese sturgeon and improve the efficiency of breeding and reproduction are still key issues that need to be addressed in the protection of the Chinese sturgeon. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide a Chinese sturgeon health level assessment system based on blood indicators, which can accurately assess the health status of the Chinese sturgeon.

[0004] The technical solution adopted by the present invention to solve the technical problem is: to provide a Chinese sturgeon health level assessment system based on blood indicators, comprising:

[0005] A blood sample processing module is used to process the blood sample of the Chinese sturgeon to obtain a serum sample of the Chinese sturgeon;

[0006] A determination module, for determining the serum sample of the Chinese sturgeon using a fully automatic biochemical analyzer to obtain blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records and serum biochemical enzyme observation records;

[0007] A selection module is used to select a blood index from blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records and serum biochemical enzyme observation records respectively;

[0008] A scoring module, used to score the selected blood indicators respectively;

[0009] The evaluation module evaluates the health level of the Chinese sturgeon based on the scores of blood indicators.

[0010] The blood sample processing module comprises:

[0011] A static unit, used for injecting the blood sample of the Chinese sturgeon into the first centrifuge tube and allowing it to stand for 4 hours at 4°C;

[0012] A centrifuge unit, used for centrifuging the blood sample in the first centrifuge tube after standing at a speed of 3000 r / min for 15 min;

[0013] The pipette unit is used to transfer the supernatant of the blood sample after centrifugation in the first centrifuge tube into the second centrifuge tube to obtain a serum sample of the Chinese sturgeon.

[0014] The selection module comprises:

[0015] A first selection unit is used to select a glucose index from the blood sugar observation record;

[0016] The second selection unit is used to select the high-density lipoprotein index from the blood lipid observation record;

[0017] The third selection unit is used to select the albumin index from the serum protein observation records;

[0018] The fourth selection unit is used to select the creatinine index from the metabolite observation records;

[0019] The fifth selection unit is used to select the aspartate / alanine aminotransferase ratio indicator from the serum biochemical enzyme observation records.

[0020] The scoring module includes:

[0021] A determination unit, used to determine the scoring segment where the selected blood index is located;

[0022] The scoring unit is used to score the selected blood index according to the determined scoring segment.

[0023] The evaluation module adopts F=W Glu ·H Glu +W ALB ·H ALB +W HDL ·H HDL +W CR ·H CR +W AST / ALT ·H AST / ALT The health level of Chinese sturgeon was evaluated, where F was the health level score of Chinese sturgeon, H was Glu The score of blood index selected for blood glucose observation record, H ALB is the score of blood indexes selected from blood lipid observation records, H HDL is the score of blood indexes selected from the serum protein observation records, H CR is the score of the blood index selected from the metabolite observation records, HAST / ALT is the score of blood indexes selected from the serum biochemical enzyme observation records, W Glu ,W HDL ,W ALB ,W CR ,W AST / ALT are all weights and satisfy W Glu +W HDL +W ALB +W CR +W AST / ALT =1.

[0024] Beneficial Effects

[0025] Due to the adoption of the above technical scheme, the present invention has the following advantages and positive effects compared with the prior art: the present invention selects glucose level as an indicator for evaluating the healthy range of blood sugar, selects high-density lipoprotein as an indicator for evaluating the healthy range of blood sugar, selects albumin as an indicator for evaluating the healthy range of serum protein, selects creatinine as an indicator for evaluating the healthy range of metabolites, and selects the aspartate / alanine aminotransferase ratio as an indicator for evaluating the healthy range of serum biochemical enzymes, and evaluates the health level of Chinese sturgeon based on the detected glucose, high-density lipoprotein, albumin, creatinine and aspartate / alanine aminotransferase ratio. This method can timely detect physiological abnormalities of Chinese sturgeon, so as to provide a basis for its treatment, and help optimize the breeding management and field protection strategy of Chinese sturgeon. In addition, by monitoring blood indicators, the occurrence of diseases can also be prevented and the survival rate of Chinese sturgeon can be improved. DETAILED DESCRIPTION

[0026] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.

[0027] The embodiment of the present invention relates to a Chinese sturgeon health level assessment system based on blood indicators, comprising:

[0028] A blood sample processing module is used to process the blood sample of the Chinese sturgeon to obtain a serum sample of the Chinese sturgeon;

[0029] A determination module, for determining the serum sample of the Chinese sturgeon using a fully automatic biochemical analyzer to obtain blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records and serum biochemical enzyme observation records;

[0030] A selection module is used to select a blood index from blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records and serum biochemical enzyme observation records respectively;

[0031] A scoring module, used to score the selected blood indicators respectively;

[0032] The evaluation module evaluates the health level of the Chinese sturgeon based on the scores of blood indicators.

[0033] Among them, the blood sample processing module includes:

[0034] A static unit, used for injecting the blood sample of the Chinese sturgeon into the first centrifuge tube and allowing it to stand for 4 hours at 4°C;

[0035] A centrifuge unit, used for centrifuging the blood sample in the first centrifuge tube after standing at a speed of 3000 r / min for 15 min;

[0036] The pipette unit is used to transfer the supernatant of the blood sample after centrifugation in the first centrifuge tube into the second centrifuge tube to obtain a serum sample of the Chinese sturgeon.

[0037] Select modules include:

[0038] A first selection unit is used to select a glucose index from the blood sugar observation record;

[0039] The second selection unit is used to select the high-density lipoprotein index from the blood lipid observation record;

[0040] The third selection unit is used to select the albumin index from the serum protein observation records;

[0041] The fourth selection unit is used to select the creatinine index from the metabolite observation records;

[0042] The fifth selection unit is used to select the aspartate / alanine aminotransferase ratio indicator from the serum biochemical enzyme observation records.

[0043] The scoring module includes:

[0044] A determination unit, used to determine the scoring segment where the selected blood index is located;

[0045] The scoring unit is used to score the selected blood index according to the determined scoring segment.

[0046] The evaluation module adopts F=W Glu ·H Glu +W ALB ·H ALB +W HDL ·H HDL +WCR ·H CR +W AST / ALT ·H AST / ALT The health level of Chinese sturgeon was evaluated, where F was the health level score of Chinese sturgeon, H was Glu The score of blood index selected for blood glucose observation record, H ALB is the score of blood indexes selected from blood lipid observation records, H HDL is the score of blood indexes selected from the serum protein observation records, H CR is the score of the blood index selected from the metabolite observation records, H AST / ALT is the score of blood indexes selected from the serum biochemical enzyme observation records, W Glu ,W HDL ,W ALB ,W CR ,W AST / ALT are all weights and satisfy W Glu +W HDL +W ALB +W CR +W AST / ALT =1.

[0047] The present invention is further illustrated by a specific embodiment below.

[0048] The breeding environment of Chinese sturgeon in this embodiment is as follows:

[0049] The breeding pond is an outdoor rectangular cement pool (13×8×2.5m), with circulating water breeding, the water flows in a counterclockwise direction, the breeding water is purified groundwater, and a wheeled aerator is used for oxygenation. The breeding water body is stable and in good condition, the water temperature is controlled at (22±1)℃, the dissolved oxygen content is ≥7mg / L, the ammonia nitrogen content is ≤0.02mg / L, the pH is 7.8-8.2, and the water transparency is 60-70cm. The breeding water body is changed every day, and the water change method is to drain water while taking in fresh seawater, and the water change volume accounts for about 1 / 3 of the total water body to ensure stable water quality.

[0050] Blood samples were collected from Chinese sturgeons by using a 20 mL disposable sterile syringe to draw 10 mL of blood from the caudal vein or caudal artery behind the anal fin of the Chinese sturgeon. The blood should be collected when the Chinese sturgeon is in a stable state to reduce the impact of stress response on the indicators.

[0051] After the blood sample collection is completed, the blood sample of the Chinese sturgeon is processed by the blood sample processing module to obtain the serum sample of the Chinese sturgeon. First, the blood is slowly injected into 5 2mL centrifuge tubes, and the centrifuge tubes are placed at 4°C for 4 hours through a static unit (such as a refrigerator). After the serum is precipitated, it is centrifuged at a speed of 3000r / min for 15 minutes using a high-speed refrigerated centrifuge unit, and then the supernatant of the blood sample in the centrifuge tube is transferred to another 2mL centrifuge tube using a pipette unit to obtain a serum sample. Finally, the serum sample is measured by a fully automatic biochemical analyzer to obtain blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records, and serum biochemical enzyme observation records.

[0052] In this embodiment, the blood sugar observation records include the glucose (mmol / L) index; the blood lipid observation records include the total cholesterol (mmol / L) index, the triglyceride (mmol / L) index, the high-density lipoprotein (mmol / L) index and the low-density lipoprotein (mmol / L) index; the serum protein observation records include the albumin (g / L) index, the globulin (g / L) index, the albumin-globulin ratio (%) index, the total protein (g / L) index and the apolipoprotein (g / L) index; the metabolite observation records include the total bile acid (μmmol / L) index, the urea nitrogen (μmmol / L) index, the uric acid (μmmol / L) index, and the L) index, creatinine (μmmol / L) index, total bilirubin (μmmol / L) index, direct bilirubin (μmmol / L) index, indirect bilirubin (μmmol / L) index; serum biochemical enzyme observation records include aspartate aminotransferase (U / L) index, alanine aminotransferase (U / L) index, aspartate / alanine aminotransferase ratio (%) index, glutamate transferase (U / L) index, cholinesterase (U / L) index, alkaline phosphatase (U / L) index, lactate dehydrogenase (U / L) index, α-hydroxybutyrate dehydrogenase (U / L) index, creatine kinase (U / L) index, creatine kinase isoenzyme (U / L) index.

[0053] In this embodiment, 5 indicators are selected from the above 27 blood biochemical indicators of the marine cultured Chinese sturgeon reserve parents through the selection module for subsequent evaluation of the health level of the Chinese sturgeon.

[0054] Blood sugar, that is, glucose in the blood, is a key substance for maintaining fish body function. In this embodiment, the glucose index is selected as an indicator for evaluating the healthy range of blood sugar to reflect the energy metabolism state of Chinese sturgeon. High-density lipoprotein is a basic substance for blood lipid metabolism, and a high value is more conducive to the health of the fish. In this embodiment, the high-density lipoprotein index is selected as an indicator for evaluating the healthy range of blood lipids to reflect the lipid metabolism and nutritional status of Chinese sturgeon. Albumin can reflect the nutritional status of Chinese sturgeon. The albumin index is selected as an indicator for evaluating the healthy range of serum protein, which is closely related to the immune status and nutritional level of Chinese sturgeon. Creatinine is a product of muscle metabolism of Chinese sturgeon and is an ideal indicator for evaluating the renal function of Chinese sturgeon. The creatinine index is selected as an indicator for evaluating the healthy range of metabolites to reflect the renal function and overall metabolic state of Chinese sturgeon. The aspartate / alanine aminotransferase ratio is an ideal indicator for evaluating the liver function of Chinese sturgeon. The aspartate / alanine aminotransferase ratio index is selected as an indicator for evaluating the healthy range of serum biochemical enzymes to reflect the health status of the liver of Chinese sturgeon.

[0055] The scoring module was used to score the five blood indicators selected above. Table 1 shows the scoring criteria for the five blood indicators to evaluate the health level of Chinese sturgeon.

[0056] Table 1 Scoring criteria for 5 blood indicators

[0057]

[0058]

[0059] Assuming that the glucose index of the Chinese sturgeon measured in this embodiment is 4.28 mmol / L, the high-density lipoprotein index is 0.14 mmol / L, the albumin index is 11.58 g / L, the creatinine index is 12.38 μmol / L, and the aspartate / alanine aminotransferase ratio index is 88.86%, then the score of the glucose index of the Chinese sturgeon is 4 points, the score of the high-density lipoprotein index is 4 points, the score of the albumin index is 3 points, the score of the creatinine index is 3 points, and the score of the aspartate / alanine aminotransferase ratio index is 1 point.

[0060] Finally, through F = W Glu ·H Glu +W ALB ·H ALB +W HDL ·H HDL +W CR ·H CR +W AST / ALT ·H AST / ALT The health status of Chinese sturgeon was assessed, among which W Glu ,W HDL ,W ALB ,W CR ,WAST / ALT are all weights and satisfy W Glu +W HDL +W ALB +W CR +W AST / ALT =1, in this embodiment, W Glu ,W HDL ,W ALB ,W CR ,W AST / ALT They are 0.15, 0.2, 0.2, 0.15 and 0.3 respectively. Glu is the score of glucose index, H ALB is the score of high-density lipoprotein index, H HDL is the score of albumin index, H CR is the score of creatinine index, H AST / ALT is the score of the aspartate / alanine aminotransferase ratio index. According to the above formula, the health level score of the Chinese sturgeon in this embodiment can be obtained as F=2.75. Combined with the Chinese sturgeon health assessment level table shown in Table 2, it can be concluded that the health level of the Chinese sturgeon in this embodiment is medium. Generally, in the daily management of Chinese sturgeons, physical examinations can be performed once in the first half of the year and once in the second half of the year, and the health level of all Chinese sturgeons can be evaluated by measuring blood indicators.

[0061] Table 2 Health assessment level of Chinese sturgeon

[0062] Total score Health Level F＜1.1 Very bad 1.1≤F＜2.3 Poor 2.3≤F＜3.5 medium 3.5≤F＜4.9 good 4.9≤F≤5 very good

[0063] It is not difficult to find that the present invention selects glucose level as an indicator for evaluating the healthy range of blood sugar, selects high-density lipoprotein as an indicator for evaluating the healthy range of blood sugar, selects albumin as an indicator for evaluating the healthy range of serum protein, selects creatinine as an indicator for evaluating the healthy range of metabolites, and selects the aspartate / alanine aminotransferase ratio as an indicator for evaluating the healthy range of serum biochemical enzymes, and evaluates the health level of Chinese sturgeon based on the detected glucose, high-density lipoprotein, albumin, creatinine and aspartate / alanine aminotransferase ratio. This method can timely discover the physiological abnormalities of Chinese sturgeon, so as to provide a basis for its treatment, and help optimize the breeding management and field protection strategy of Chinese sturgeon. In addition, by monitoring blood indicators, the occurrence of diseases can also be prevented and the survival rate of Chinese sturgeon can be improved.

Claims

1. A Chinese sturgeon health level assessment system based on blood indicators, characterized in that: include: A blood sample processing module is used to process the blood sample of the Chinese sturgeon to obtain a serum sample of the Chinese sturgeon; A determination module, for determining the serum sample of the Chinese sturgeon using a fully automatic biochemical analyzer to obtain blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records and serum biochemical enzyme observation records; A selection module is used to select a blood index from blood sugar observation records, blood lipid observation records, serum protein observation records, metabolite observation records and serum biochemical enzyme observation records respectively; A scoring module, used to score the selected blood indicators respectively; The evaluation module evaluates the health level of the Chinese sturgeon based on the scores of blood indicators.

2. The Chinese sturgeon health level assessment system based on blood indicators according to claim 1 is characterized in that: The blood sample processing module comprises: A static unit is used to inject the blood sample of Chinese sturgeon into the first centrifuge tube and let it stand for 4 hours at 4°C; a centrifuge unit is used to centrifuge the blood sample in the first centrifuge tube after standing at a speed of 3000r / min for 15 minutes; The pipette unit is used to transfer the supernatant of the blood sample after centrifugation in the first centrifuge tube into the second centrifuge tube to obtain a serum sample of the Chinese sturgeon.

3. The Chinese sturgeon health level assessment system based on blood indicators according to claim 1 is characterized in that: The selection module comprises: A first selection unit is used to select a glucose index from the blood sugar observation record; The second selection unit is used to select the high-density lipoprotein index from the blood lipid observation record; The third selection unit is used to select the albumin index from the serum protein observation records; The fourth selection unit is used to select the creatinine index from the metabolite observation records; The fifth selection unit is used to select the aspartate / alanine aminotransferase ratio indicator from the serum biochemical enzyme observation records.

4. The Chinese sturgeon health level assessment system based on blood indicators according to claim 1 is characterized in that: The scoring module includes: A determination unit, used to determine the scoring segment where the selected blood index is located; The scoring unit is used to score the selected blood index according to the determined scoring segment.

5. The Chinese sturgeon health level assessment system based on blood indicators according to claim 1 is characterized in that: The evaluation module adopts F=W Glu ·H Glu +W ALB ·H ALB +W HDL ·H HDL +W CR ·H CR +W AST / ALT ·H AST / ALT The health level of Chinese sturgeon was evaluated, where F was the health level score of Chinese sturgeon, H was Glu The score of blood index selected for blood glucose observation record, H ALB is the score of blood indexes selected from blood lipid observation records, H HDL is the score of blood indexes selected from the serum protein observation records, H CR is the score of the blood index selected from the metabolite observation records, H AST / ALT is the score of blood indexes selected from the serum biochemical enzyme observation records, W Glu ,W HDL ,W ALB ,W CR ,W AST / ALT are all weights and satisfy W Glu +W HDL +W ALB +W CR +W AST / ALT =1.

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