Rhizoma polygonati medicinal material and processing method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicinal material processing, in particular to a rhizoma polygonati medicinal material and a processing method thereof.The processing method comprises the steps that the rhizoma polygonati medicinal material is subjected to root hair removal, cleaned, placed in a steamer to be steamed for 2 h in a water isolation mode, taken out, sliced and dried for 6-9 h at the temperature of 50-70 DEG C, rhizoma polygonati raw decoction pieces are obtained, vine tea extraction juice is added into the rhizoma polygonati decoction pieces according to a certain solid-to-liquid ratio, covered moistening is conducted for 8-10 h, and the rhizoma polygonati raw decoction pieces are obtained. After the ampelopsis grossedentata extracting juice is absorbed, putting the ampelopsis grossedentata extracting juice into a steamer to be steamed for 2-5 hours in a water isolation manner, taking out the ampelopsis grossedentata extracting And steaming for 3-6 times according to the steps to obtain the prepared rhizoma polygonati decoction pieces. The processing method is simple, feasible and easy to operate. By adopting the optimized process, the oxidation resistance of the prepared rhizoma polygonati decoction pieces is improved by 39.68%, the content of 5-hydroxymethylfurfural is reduced by 64.48%, the internal quality of rhizoma polygonati processed products is improved, and a new thought is provided for the rhizoma polygonati processing process.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicinal material processing, in particular to a polygonatum medicinal material and a processing method thereof. Background Art

[0002] As a traditional Chinese medicinal material, Polygonatum sibiricum has a history of more than 2,000 years in my country. It is the dried rhizome of Polygonatum kingianum Coll, et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua, which belong to the Liliaceae family. Polygonatum sibiricum is sweet and mild in nature, and enters the spleen, lung and kidney meridians. It was listed in the catalog of medicine and food homology in 2002. It is a good medicine for replenishing qi and nourishing yin, strengthening the spleen and moistening the lungs.

[0003] Raw Polygonatum has a numbing effect on the tongue and is irritating to the throat. It cannot be used directly. It needs to be processed to reduce its irritation and enhance its efficacy. With the in-depth study of the processing technology of Polygonatum, it is found that the processing technology has a profound impact on the chemical composition and pharmacological effects of Polygonatum. Some researchers have systematically explained the changes in the chemical composition of Yunnan Polygonatum before and after processing, providing new ideas for the analysis of differential substances in raw and cooked Yunnan Polygonatum decoction pieces.

[0004] There are many patents on the processing of polygonatum sibiricum. Among them, CN116832117A discloses a processing process for polygonatum sibiricum. This process prepares polygonatum sibiricum with uniform drying, better texture and less loss of effective ingredients through pre-drying, slicing and gradient cooling drying steps. It overcomes the defects of uneven drying or excessively high drying temperature that make the processed polygonatum hard and brittle and inconvenient for medicine. In addition, gradient cooling drying is conducive to shortening the gradient drying time and better moisture control.

[0005] CN118593628A discloses a processing technology of using Chinese wolfberry and roasted Polygonatum, and optimizes the amount of Chinese wolfberry, steaming time and steaming times to determine the best processing technology of Chinese wolfberry and roasted Polygonatum, which can greatly improve the extraction rate of effective ingredients of roasted Polygonatum medicinal materials and provide guarantee for its clinical efficacy. CN118490782A discloses a processing method of Polygonatum acutiloba, which can complete the processing of nine-steamed and nine-dried Polygonatum acutiloba through the steps of selection, cutting, steaming, initial steaming, repeated steaming, high-temperature aroma, etc., reducing the participation of a large number of mechanical equipment in the production process of Polygonatum acutiloba, retaining the nutrients inside Polygonatum acutiloba as much as possible, and ensuring the effective production of Polygonatum acutiloba.

[0006] At present, there are many methods for processing polygonatum, but no research has been reported on improving the antioxidant function of processed polygonatum and reducing the content of 5-hydroxymethylfurfural. How to simultaneously improve the antioxidant function of processed polygonatum and reduce the content of 5-hydroxymethylfurfural has become one of the technical problems that need to be solved urgently. Summary of the invention

[0007] In view of this, the present invention proposes a polygonatum medicinal material and a processing method thereof, aiming to improve the antioxidant capacity of the processed polygonatum and reduce the content of 5-hydroxymethylfurfural.

[0008] The technical solution of the present invention is achieved as follows: The present invention provides a method for preparing a polygonatum medicinal material, comprising:

[0009] Step 1: Remove the roots of the polygonatum sibiricum, wash it, steam it in a steamer for 2 hours, take it out and slice it, and dry it at 50-70°C for 6-9 hours to obtain the raw polygonatum sibiricum slices;

[0010] Step 2: crush the rattan tea, sieve it, add water at 100°C and heat extract for 1-2h, filter to obtain an extract, and concentrate the extract to 0.5-2 times the weight of the rattan tea to obtain the rattan tea extract juice;

[0011] Step 3, add the extract of rattan tea to the raw slices of polygonatum, and steam the raw slices of polygonatum in a steamer for 2-5 hours after being simmered for 8-10 hours, take out, and dry at 50-70°C for 6-9 hours;

[0012] Step 4: Repeat step 3 3-6 times to obtain the prepared Polygonatum sibiricum decoction slices.

[0013] In the above embodiment, the purpose of removing roots and washing is to remove as much debris and residual harmful substances as possible on the surface to ensure the quality of the processed medicinal materials. Steaming can effectively soften the medicinal materials and promote the release of effective ingredients to prepare for subsequent drying. The drying temperature of 50-70°C and the drying time of 6-9h are helpful to maintain the stability of the active ingredients in Polygonatum sibiricum, especially polysaccharides and flavonoids, while avoiding the formation of 5-hydroxymethylfurfural caused by high temperature. The drying time of 6-9 hours is sufficient to make the medicinal materials reach an appropriate degree of dryness while avoiding the loss of effective ingredients that may be caused by overdrying.

[0014] Rattan tea has antioxidant properties and can provide natural antioxidant ingredients. The purpose of heat extraction is to effectively dissolve the active ingredients in rattan tea. 100℃ hot water extraction helps to quickly dissolve the active ingredients in rattan tea, and an extraction time of 1-2 hours can ensure sufficient extraction efficiency. The concentrated extract can increase the concentration of the active ingredients in rattan tea, allowing it to better combine with the medicinal material of Polygonatum odoratum and enhance the antioxidant effect.

[0015] Steaming for 2-5 hours can further activate the active ingredients in polygonatum, and promote the better combination of rattan tea extract and polygonatum, while avoiding the loss of ingredients caused by too long steaming. 2-5 hours can effectively control the temperature and humidity and prevent excessive generation of 5-hydroxymethylfurfural.

[0016] Repeating the steaming process 3-6 times can ensure that the rattan tea extract and polygonatum are fully integrated, gradually improve the antioxidant properties of polygonatum, and effectively reduce the possible 5-hydroxymethylfurfural content. Repeating the process 3-6 times can ensure that the medicinal ingredients are fully transformed, while avoiding the loss of medicinal ingredients due to excessive processing.

[0017] In some embodiments, in step 2, the water used for hot extraction is 10-30 times the mass of rattan tea.

[0018] In some embodiments, in step 2, the water used for hot extraction is 20 times the weight of the rattan tea, the hot extraction time is 1.5 hours, and the extract is concentrated to 1 times the weight of the rattan tea.

[0019] In some embodiments, in step three, the mass ratio of rattan tea extract juice to raw polygonatum slices is 100:4.

[0020] This ratio ensures that the antioxidant components of rattan tea can fully penetrate and interact with the effective components of polygonatum, promoting the efficacy of polygonatum, while avoiding other adverse reactions caused by excessive components of rattan tea.

[0021] In some embodiments, in step 1, the drying temperature is 60° C. and the drying time is 8 hours.

[0022] In some embodiments, in step three, the steaming time is 4 hours.

[0023] In some embodiments, in step 4, step 3 is repeated 5 times.

[0024] In a second aspect, the present invention further provides a polygonatum medicinal material, which is prepared by the above-mentioned processing method.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The invention provides a processing method for polygonatum sibiricum. The processing method is simple and easy to operate, and the antioxidant capacity of the obtained polygonatum sibiricum decoction pieces is improved by 39.68%, and the content of 5-hydroxymethylfurfural is reduced by 64.48%, thereby providing a new idea for the processing technology of gold. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 The figure is a comparison chart of the antioxidant capacity and 5-hydroxymethylfurfural content of the prepared polygonatum sibiricum slices prepared in the examples of the present invention and the comparison. DETAILED DESCRIPTION

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the embodiments of the present invention belong. If the definitions set forth in this section are contrary to or otherwise inconsistent with the definitions set forth in the patents, patent applications, published patent applications, and other publications incorporated herein by reference, the definitions listed in this section take precedence over the definitions incorporated herein by reference.

[0031] Unless otherwise specified, the methods used in the following examples are all conventional methods. Unless otherwise specified, the materials, reagents and instruments used are all conventional materials, reagents and instruments in the art, and can be obtained by those skilled in the art through commercial channels.

[0032] When an amount, concentration or other value or parameter is expressed as a range, a preferred range or a range defined by a series of upper preferred values ​​and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted as including the range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values ​​and all integers and fractions within the range. In the present specification and claims, range definitions can be combined and / or interchanged, and if not otherwise stated, these ranges include all subranges contained therein.

[0033] In the processing of Polygonatum sibiricum, 5-hydroxymethylfurfural is a component that needs attention. It is usually formed during high-temperature treatment, especially when Polygonatum sibiricum is overheated during drying or processing, a higher content may be produced. 5-Hydroxymethylfurfural has certain antioxidant properties. As an active small molecule compound, it can play an antioxidant role by reacting with free radicals. However, it also has some potential toxicity, especially in high concentrations or long-term exposure. Therefore, controlling 5-hydroxymethylfurfural in Polygonatum sibiricum and ensuring the antioxidant properties of Polygonatum sibiricum are one of the technical problems that need to be solved urgently.

[0034] Tengcha is a wild vine plant of the Vitaceae family and the genus Ampelopsis, which is widely distributed in southwest my country, Shaanxi, Gansu, Hubei and other places. Tengcha contains rich nutrients and bioactive ingredients, among which polyphenols are considered to be one of the main active ingredients of tengcha, with physiological activities such as anti-oxidation, hypoglycemic, hypolipidemic, anti-inflammatory and antibacterial. Adding tengcha extract during the processing of polygonatum is beneficial to improve the intrinsic quality of polygonatum decoction pieces.

[0035] This application optimizes the processing technology of Polygonatum sibiricum through rattan tea. On the premise of ensuring and improving the antioxidant properties of the processed Polygonatum sibiricum medicinal materials, it also significantly reduces the content of 5-hydroxymethylfurfural, thereby comprehensively improving the therapeutic value and safety of Polygonatum sibiricum.

[0036] The technical solution of the present application is now described in detail through the following examples and comparative examples.

[0037] The present invention also provides a detection method for 5-hydroxymethylfurfural, which is as follows:

[0038] 1.1 Instrument

[0039] High performance liquid chromatograph: UltiMate 3000, Thermo Fisher Scientific, USA.

[0040] Electric heating blast drying oven: Model GXZ-9246MBE, purchased from Shanghai Boxun Industrial Co., Ltd.

[0041] The 1 / 100,000 electronic balance, model AB135 S, was purchased from Mettler-Toledo.

[0042] Water purifier: Model: Option R7 Flex2, purchased from Veolia Water Treatment Technology (Shanghai) Co., Ltd.

[0043] 1.2 Reagents

[0044] Standard: 5-Hydroxymethylfurfural standard, for content detection.

[0045] Methanol: chromatographic grade reagent; pure water is primary test water prepared by Option R7 Flex2.

[0046] 1.3 Preparation of 5-hydroxymethylfurfural standard solution:

[0047] Accurately weigh an appropriate amount of 5-hydroxymethylfurfural standard, add methanol to dissolve and dilute to a certain concentration, accurately pipette the diluted 5-hydroxymethylfurfural standard solution into a 10mL volumetric flask, add water to the scale line, shake well, filter, and obtain.

[0048] 1.4 Preparation of test solution:

[0049] Accurately weigh 0.1 g of prepared Polygonatum sibiricum powder, add 25 mL of water, shake well, treat with ultrasound for 30 min, take out, cool, filter through a 0.45 μm water filter membrane, and then wait for machine testing.

[0050] 1.5 Chromatographic conditions

[0051] Agilent SB-Aq C18 chromatographic column (4.6 mm×250 mm, 5 μm); column temperature: 30°C; detection wavelength: 285 nm; injection volume: 10 μL; mobile phase: methanol-0.1% phosphoric acid solution, gradient elution program: see Table 1.

[0052] Table 1 Elution gradient program

[0053] Time / min Methanol 0.1% phosphoric acid solution 0 8 92 30 8 92

[0054] 1.6 5-Hydroxymethylfurfural content detection

[0055] The treated samples and reference substances were tested on the instrument to obtain the peak areas of 5-hydroxymethylfurfural respectively. A standard curve was drawn with concentration as the horizontal axis and the peak area of ​​5-hydroxymethylfurfural as the vertical axis. The test results of the samples were automatically calculated by the instrument software.

[0056] In other aspects, the present invention also provides a method for detecting the antioxidant capacity (ORAC) of processed Polygonatum sibiricum slices, the steps are as follows:

[0057] 2.1 Instruments

[0058] Multi-function microplate reader model: Synergy H1, BioTek company.

[0059] Automatic pipetting and dispensing system model: Bioteck Precesion PRC 384U BioTek company.

[0060] Pure water / ultrapure water integrated system model: Option R7 Flex2, purchased from Veolia Water Treatment Technology (Shanghai) Co., Ltd.

[0061] Pipette (100 μL, 1000 μL) model: Eppendorf, Germany.

[0062] 2.2 Reagents

[0063] Fluorescein sodium salt FL, dipotassium hydrogen phosphate (K2HPO4), sodium dihydrogen phosphate (NaH2PO4), water-soluble vitamin E standard, AAPH, gallic acid, uric acid.

[0064] 2.3 Solution preparation

[0065] 0.75M K2HPO4: 130g potassium dihydrogen phosphate dissolved in 1L ultrapure water.

[0066] 0.75M NaH2PO4: Dissolve 90g sodium dihydrogen phosphate in 1L ultrapure water.

[0067] Phosphate buffer solution working solution (PH=7.4): 81 mL of K2HPO4 stock solution and 19 mL of NaH2PO4 stock solution were measured respectively, mixed and then made up to 1000 mL with ultrapure water to obtain a 75 mM, pH 7.4 buffer solution.

[0068] Fluorescein sodium salt solution: weigh 0.0456 g of fluorescein sodium salt and dilute to 100 mL of phosphate buffer, store in a dark place to obtain a stock solution; take 1000 μL of the stock solution and dilute to 100 mL with phosphate buffer, store in a dark place to obtain an intermediate solution; take 1000 μL of the intermediate solution and dilute to 100 mL with phosphate buffer, store in a dark place to obtain a working solution.

[0069] Preparation of Trolox standard solution: Take 0.0125g Trolox and dilute to 50ml phosphate buffer to obtain 1000μM stock solution. Dilute with phosphate buffer solution to 12.5, 25, 50, 100, 200, 300μM working solution.

[0070] AAPH solution: 0.414 g AAPH is completely dissolved in 10 mL pH 7.4, 75 mM phosphate buffer solution to obtain a 153 mM solution, which is stored in an ice bath. (Prepare and use immediately)

[0071] Gallic acid solution: 0.017 g of gallic acid was diluted to 100 mL of phosphate buffer to obtain a 1000 μM stock solution, which was then diluted to obtain a 50 μM working solution.

[0072] 2.4 Preparation of test solution:

[0073] Take 1 g of processed Polygonatum sibiricum powder, add 25 mL of 95% ethanol solution, shake well, ultrasonically treat for 30 minutes, and centrifuge to obtain the product.

[0074] 2.5 Antioxidant capacity test

[0075] The blank, sample and standard antioxidant (Trolox) were mixed with the fluorescein solution respectively and incubated in a microplate reader at 37°C for 30 minutes. Then, the AAPH solution was quickly added and the measurement was started in the microplate reader. The fluorescence intensity was recorded using the software equipped with the fluorescence microplate reader. The initial fluorescence intensity value was recorded as f0. The measurement was then performed every two minutes for 120 minutes. The fluorescence intensities were recorded as f0, f1, f2...f61 respectively.

[0076] The relevant parameters of the instrument and method are: excitation wavelength 485±20nm, emission wavelength 528±20nm, sensitivity automatic, light source halogen lamp. Blank, sample, standard antioxidant (Trolox) and analytical control sample (gallic acid) 20μL each, fluorescein sodium salt solution 160μL, AAPH solution 20μL.

[0077] The calculation formula of the area under the fluorescence decay curve (AUC) is AUC = f0 / f0 + 2 × (f1 / f0 + ... + f60 / f0) + f61 / f0. The integral area under the fluorescence decay curve under the action of antioxidants is deducted from the area under the blank curve without antioxidants to obtain the net area under the curve (Net AUC). The standard curve is drawn with the content of Trolox standard solution as the abscissa and the corresponding net area of ​​the curve (Net AUC) as the ordinate to obtain the regression equation, and the Net AUC generated by the sample is substituted into the equation to obtain the content.

[0078] The following specific examples and comparative examples are used to verify the different processing methods of Polygonatum odoratum.

[0079] Example 1

[0080] Preparation of rattan tea extract juice: crush the rattan tea, sieve, add 20 times water and heat extract at 100℃ for 1.5h, filter to obtain the extract, and concentrate the extract to 1 times the weight of the rattan tea to obtain the rattan tea extract juice.

[0081] Step 1: Remove the roots of the Polygonatum sibiricum, wash it, steam it in a steamer for 2 hours, take it out, slice it, and dry it at 60°C for 8 hours to obtain the raw Polygonatum sibiricum slices.

[0082] Step 2: Add the rattan tea extract juice to the Polygonatum sibiricum slices at a solid-liquid ratio of 4:100, and simmer for 10 hours until the rattan tea extract juice is absorbed. Then, steam it in a steamer for 4 hours, take it out, and dry it at 60°C for 8 hours.

[0083] Step 3: Repeat the above step 2 for 5 times to obtain the prepared Polygonatum sibiricum decoction slices.

[0084] Comparative Example 1

[0085] Step 1: Remove the roots of the Polygonatum sibiricum, wash it, steam it in a steamer for 2 hours, take it out, slice it, and dry it at 60°C for 8 hours to obtain the raw Polygonatum sibiricum slices.

[0086] Step 2: Add rice wine to the raw slices of Polygonatum sibiricum in a solid-liquid mass ratio of 4:100, and simmer for 10 hours until the rice wine is absorbed. Then, place the raw slices of Polygonatum sibiricum in a steamer and steam for 4 hours, take them out, and dry them at 60°C for 8 hours.

[0087] Step 3: Repeat the above step 2 for 5 times to obtain the prepared Polygonatum sibiricum decoction slices.

[0088] Comparative Example 2

[0089] Step 1: Remove the roots of the Polygonatum sibiricum, wash it, steam it in a steamer for 2 hours, take it out, slice it, and dry it at 60°C for 8 hours to obtain the raw Polygonatum sibiricum slices.

[0090] Step 2: Add water to the raw slices of Polygonatum sibiricum at a solid-liquid mass ratio of 4:100, and let it soak for 10 hours until the rice wine is absorbed. Then, place the raw slices of Polygonatum sibiricum in a steamer and steam them for 4 hours, take them out, and dry them at 60°C for 8 hours.

[0091] Step 3: Repeat the above step 2 for 5 times to obtain the prepared Polygonatum sibiricum decoction slices.

[0092] The antioxidant capacity and 5-hydroxymethylfurfural content of the polygonatum sibiricum slices prepared in the above Example 1 and Comparative Examples 1 and 2 were tested respectively. The test results are shown in Table 2:

[0093]

[0094]

[0095] As can be seen from the above table, after adding rattan tea extract during the processing of polygonatum sibiricum in Example 1, its antioxidant capacity is as high as 18169.342mmol TE / mL, and the 5-hydroxymethylfurfural content is 962.9mg / Kg; compared with the steaming process (Comparative Example 2), the antioxidant capacity is increased by 39.68%, and the 5-hydroxymethylfurfural content is reduced by 64.48%, which is beneficial to improving the intrinsic quality of the processed polygonatum sibiricum product.

[0096] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing Polygonatum sibiricum medicinal material, characterized in that: The steps include: Step 1: Remove the roots of the polygonatum sibiricum, wash it, steam it in a steamer for 2 hours, take it out and slice it, and dry it at 50-70°C for 6-9 hours to obtain the raw polygonatum sibiricum slices; Step 2: crush the rattan tea, sieve it, add water at 100°C and heat extract for 1-2h, filter to obtain an extract, and concentrate the extract to 0.5-2 times the weight of the rattan tea to obtain the rattan tea extract juice; Step 3, add the extract of rattan tea to the raw slices of polygonatum, and steam the raw slices of polygonatum in a steamer for 2-5 hours after being simmered for 8-10 hours, take out, and dry at 50-70°C for 6-9 hours; Step 4: Repeat step 3 3-6 times to obtain the prepared Polygonatum sibiricum decoction slices.

2. The method for preparing the medicinal material of polygonatum according to claim 1, characterized in that: In step 2, the water used for hot extraction is 10-30 times the mass of rattan tea.

3. The method for preparing the medicinal material of polygonatum according to claim 1, characterized in that: In step 2, the water used for hot extraction is 20 times the weight of rattan tea, the hot extraction time is 1.5 hours, and the extract is concentrated to 1 times the weight of rattan tea.

4. The method for preparing the medicinal material of polygonatum according to claim 1, characterized in that: In step three, the mass ratio of rattan tea extract juice to raw polygonatum slices is 100:

4.

5. The method for preparing the medicinal material of polygonatum according to claim 1, characterized in that: In step 1, the drying temperature is 60° C. and the drying time is 8 h.

6. The method for preparing the medicinal material of polygonatum according to claim 1, characterized in that: In step 3, the steaming time is 4 hours.

7. The method for processing polygonatum sibiricum medicinal material according to claim 1, characterized in that: In step 4, repeat step 3 5 times.

8. A polygonatum medicinal material, characterized in that: The product is prepared by the preparation method described in any one of claims 1 to 7.

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