Soil dehydrogenase detection equipment and detection method

Abstract

The invention discloses soil dehydrogenase detection equipment and a detection method, and belongs to the field of dehydrogenase activity detection. The soil dehydrogenase detection equipment comprises a base, and further comprises a first accommodating cavity, a second accommodating cavity, a first rotating shaft and a second rotating shaft, the rotating shaft is rotationally arranged in the first accommodating cavity; the motor is fixedly connected to the lower end of the base, and the output end of the motor is fixedly connected with a rotating shaft; the high-pressure cavity is formed in the base, the high-pressure cavity and the first containing cavity are coaxially arranged, flexible protrusions are symmetrically arranged between the high-pressure cavity and the first containing cavity, and the flexible protrusions are fixedly connected with the base; according to the soil dehydrogenase detection device, the mixing effect of a solution and soil is effectively improved, the situation that the culture effect is affected by internal soil precipitation in the culture process is prevented, and then the soil dehydrogenase detection efficiency and the accuracy of a detection result are effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of dehydrogenase activity detection, and in particular to a soil dehydrogenase detection device and a detection method. Background Art

[0002] Soil dehydrogenase activity, as a key indicator for evaluating soil microbial activity, soil health and soil pollution level, has received more and more widespread attention. Soil dehydrogenase can catalyze redox reactions of a variety of organic substrates. Its activity level is closely related to the metabolic activity of microorganisms in the soil, and thus reflects important ecological characteristics such as soil fertility, decomposition and transformation capacity of organic matter, and soil recovery capacity after pollution. Therefore, it is crucial to detect dehydrogenase in soil. The detection methods of soil dehydrogenase mainly include chemical analysis methods such as TTC reduction method, spectrophotometry, and iodine tetrazolium chloride method. The commonly used method currently mainly uses TTC reduction method. Its principle is that TTC accepts hydrogen under the catalysis of soil dehydrogenase and is reduced to red triphenylformazan (TF). The product has a maximum absorption peak at 485nm. The change in absorbance at this wavelength is measured by a spectrophotometer to characterize the activity of soil dehydrogenase.

[0003] At present, when testing soil dehydrogenase, it is necessary to mix the soil with TTC solution and glucose solution. Due to the small amount, it is difficult to mix them by shaking the test tube, and some soil will stick to the inner wall, affecting the accuracy and efficiency of the soil dehydrogenase test results. In addition, due to the large diameter of the test tube, the liquid level of the less solution in the test tube is low, and it is heated slowly when cultured in the constant temperature box, so that the culture time is longer, which may affect the efficiency of soil dehydrogenase detection.

[0004] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0005] In order to achieve the above object, a technical solution provided by a specific embodiment of the present invention is as follows:

[0006] A soil dehydrogenase detection device comprises: a base, and also comprises: a first accommodating chamber, which is arranged in the base; a rotating shaft, which is rotatably arranged in the first accommodating chamber; a motor, which is fixedly connected to the lower end of the base, and the output end of the motor is fixedly connected to the rotating shaft; a high-pressure chamber, which is arranged in the base, and the high-pressure chamber is coaxially arranged with the first accommodating chamber, and flexible protrusions are symmetrically arranged between the high-pressure chamber and the first accommodating chamber, and the flexible protrusions are fixedly connected to the base; an extrusion block, which is symmetrically fixedly connected to the rotating shaft, and the extrusion block is used to extrude the flexible protrusions; a accommodating bottle, which is threadedly sealed and connected to the upper side of the base; a heated tube, which is arranged on the upper side of the accommodating bottle, and the diameter of the heated tube is smaller than the diameter of the accommodating bottle; a connecting tube, which is fixedly connected to the upper side of the heated tube, and the diameter of the connecting tube is larger than the diameter of the heated tube; a flexible stirring wire, which is fixedly arranged on the rotating shaft, and the flexible stirring wire is arranged in the heated tube.

[0007] Preferably, the flexible protrusion is configured to be spherical, arc-shaped grinding surfaces are symmetrically provided on the extrusion block, and a first grinding surface matching the arc-shaped grinding surface is provided on the outer side of the flexible protrusion.

[0008] In order to facilitate grinding of the bonded soil and improve the mixing effect of the soil and the solution, further, a first grinding area and a second grinding area are provided between the flexible protrusion and the spherical flexible protrusion.

[0009] In order to prevent soil impurities from being present at the bottom of the first accommodating chamber, further, an inclined plate is symmetrically fixedly connected to the rotating shaft, and the inclined plate fits the bottom of the first accommodating chamber, and the inclined plate and the extrusion block are staggered.

[0010] In order to facilitate the sedimentation and backflow of soil at the bottom of the connecting pipe, a conical surface is further provided between the heated pipe and the connecting pipe.

[0011] In order to prevent excessive soil from adhering to the receiving bottle, further, after adding TTC solution and glucose solution into the receiving bottle, the liquid level is located at the connecting tube.

[0012] In order to facilitate sampling, further, a sampling area is provided on the connecting tube.

[0013] In order to prevent the agglomerated soil from flowing into the receiving bottle, further, a filter plate sealed with the first receiving cavity is provided on the lower side of the receiving bottle.

[0014] In order to improve the mixing effect of the solution, further, the filter plate is provided with a plurality of first filter holes matching the heated tube, an arc surface is provided in the receiving bottle, and the filter plate is provided with a plurality of second filter holes matching the arc surface.

[0015] A soil dehydrogenase detection method mainly comprises the following steps:

[0016] Step 1: prepare reagents TTC, trimethylaminomethane, methanol and TPF, and configure them into a certain concentration;

[0017] Step 2: Screening the fresh soil, and adding the quantitatively screened soil into the first containing chamber;

[0018] Step 3: Connect the container bottle to the base with threads, then add appropriate amounts of TTC solution and glucose solution into the container bottle, take another base and container bottle, add soil into the first container chamber in the same manner, and then use trimethylaminomethane instead of TTC solution as a control;

[0019] Step 4: Place the container bottle in a dark room of a constant temperature box and culture for a certain period of time;

[0020] Step 5, turn on the motor, the motor drives the extrusion block to rotate through the rotating shaft to squeeze the flexible protrusion so that the volume in the first accommodating chamber becomes larger, and the internal liquid flows upward. When the extrusion block moves away from the flexible protrusion, the air pressure in the high-pressure chamber squeezes the flexible protrusion to reset, so that the volume in the first accommodating chamber becomes smaller, and the liquid in the accommodating bottle flows downward;

[0021] Step 6: The flexible stirring wire is driven by the rotating shaft to stir the liquid so that the liquid is always in a mixed state;

[0022] Step 7: After the culture is completed, add an appropriate amount of methanol to the container bottle in small amounts and multiple times, place the container bottle on an oscillator and oscillate for a certain period of time, and then filter the liquid with filter paper;

[0023] Step 8, measuring the volume of the filtrate, and placing the filtrate in a spectrophotometer to measure the absorbance at 485 nm;

[0024] Step 9. Calculate the dehydrogenase activity by comparing with the standard curve.

[0025] Compared with the prior art, the present invention provides a soil dehydrogenase detection device, which has the following beneficial effects:

[0026] 1. The soil dehydrogenase detection device is configured such that the soil and the solution are initially mixed when the extrusion block rotates in the first accommodating chamber, and when the extrusion block rotates to the flexible protrusion, the extrusion block squeezes the flexible protrusion to make the flexible protrusion concave, and the flexible protrusion moves toward the high-pressure chamber to increase the air pressure in the high-pressure chamber. The deformed flexible protrusion relatively increases the volume in the first accommodating chamber. At this time, the liquid in the accommodating bottle flows into the first accommodating chamber and mixes with the soil mixed liquid in the first accommodating chamber. When the extrusion block moves away from the flexible protrusion, the flexible protrusion is reset under the action of the high-pressure chamber. At this time, the volume in the first accommodating chamber becomes relatively smaller, and part of the mixed liquid in the first accommodating chamber is squeezed upward. The mixing effect of the soil and the solution is effectively improved by the up and down flow of the liquid. At the same time, the solution is axially stirred in conjunction with the flexible stirring wire, thereby effectively improving the mixing effect of the solution and the soil.

[0027] 2. The soil dehydrogenase detection equipment blocks the solution through the filter plate, so that the solution has a certain impact force when entering the first containing chamber, thereby effectively improving the mixing effect of the solution in the containing bottle and the solution in the first containing chamber, making the soil and the solution mixed more evenly. At the same time, through the reciprocating flow of the solution, on the one hand, the filter plate can be prevented from being blocked, and on the other hand, the fluidity of the solution can be increased, thereby improving the mixing effect of the solution.

[0028] 3. The soil dehydrogenase detection equipment places a base with a containing bottle in a constant temperature box for darkroom cultivation. During the cultivation process, the motor drives the rotating shaft to continue to rotate, so that the solution in the containing bottle is always in a mixed state, preventing the internal soil from settling and affecting the cultivation effect during the cultivation process.

[0029] 4. The soil dehydrogenase detection equipment can increase the heating area of ​​the mixed solution by setting a small diameter heating tube, so that the solution can reach the optimal culture temperature faster and reduce the required culture time, thereby effectively reducing the time for dehydrogenase detection in the soil.

[0030] The parts not involved in the device are the same as the existing technology or can be implemented by using the existing technology. The present invention effectively improves the mixing effect of the solution and the soil, prevents the internal soil precipitation from affecting the cultivation effect during the cultivation process, and thus effectively improves the efficiency of soil dehydrogenase detection and the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0032] Figure 1 This is a schematic diagram of the structure of a soil dehydrogenase detection device proposed by the present invention;

[0033] Figure 2 A cross-sectional schematic diagram of a soil dehydrogenase detection device proposed by the present invention;

[0034] Figure 3 This is a cross-sectional schematic diagram of a base in a soil dehydrogenase detection device proposed by the present invention;

[0035] Figure 4 This is a schematic structural diagram of a base, an inclined plate, and a first grinding area in a soil dehydrogenase detection device proposed by the present invention;

[0036] Figure 5 This is a schematic diagram of the structure of an extrusion block and an arc-shaped grinding surface in a soil dehydrogenase detection device proposed by the present invention;

[0037] Figure 6 This is a schematic diagram of the structure of an extrusion block and an inclined plate in a soil dehydrogenase detection device proposed by the present invention;

[0038] Figure 7 This is a schematic structural diagram of the second grinding area and the arc-shaped grinding surface in a soil dehydrogenase detection device proposed by the present invention.

[0039] In the figure: 1. base; 101. first accommodating chamber; 102. high-pressure chamber; 103. motor; 104. rotating shaft; 105. flexible protrusion; 106. first grinding surface; 107. extrusion block; 108. arc-shaped grinding surface; 109. first grinding area; 110. second grinding area; 111. inclined plate; 112. flexible stirring wire; 2. accommodating bottle; 201. filter plate; 202. first filter hole; 203. second filter hole; 204. arc-shaped surface; 205. heated tube; 206. connecting tube; 207. conical surface; 208. sampling area. DETAILED DESCRIPTION

[0040] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0041] Example 1: Reference Figure 1-Figure 7A soil dehydrogenase detection device comprises: a base 1, and also comprises: a first accommodating chamber 101, which is arranged in the base 1; a rotating shaft 104, which is rotatably arranged in the first accommodating chamber 101; a motor 103, which is fixedly connected to the lower end of the base 1, and the output end of the motor 103 is fixedly connected to the rotating shaft 104; a high-pressure chamber 102, which is arranged in the base 1, and the high-pressure chamber 102 is coaxially arranged with the first accommodating chamber 101, and a flexible protrusion 105 is symmetrically arranged between the high-pressure chamber 102 and the first accommodating chamber 101, and the flexible protrusion 105 is fixedly connected to the base 1. connection; an extrusion block 107, which is symmetrically fixedly connected to the rotating shaft 104, and the extrusion block 107 is used to extrude the flexible protrusion 105; a containing bottle 2, which is threadedly sealed and connected to the upper side of the base 1; a heated tube 205, which is arranged on the upper side of the containing bottle 2, and the diameter of the heated tube 205 is smaller than the diameter of the containing bottle 2; a connecting tube 206, which is fixedly connected to the upper side of the heated tube 205, and the diameter of the connecting tube 206 is larger than the diameter of the heated tube 205; a flexible stirring wire 112, which is fixedly arranged on the rotating shaft 104, and the flexible stirring wire 112 is arranged in the heated tube 205.

[0042] A filter plate 201 sealed with the first receiving chamber 101 is disposed on the lower side of the receiving bottle 2 .

[0043] When detecting the dehydrogenase in the soil, two bases 1 and two receiving bottles 2 are taken, and a proper amount of sieved fresh soil is added into the first receiving chamber 101 of the base 1, and then the receiving bottle 2 is installed on the base 1, and TTC solution and a proper amount of glucose solution are added into the receiving bottle 2, and the solution enters the first receiving chamber 101 through the connecting pipe 206, the temperature receiving pipe 205 and the filter plate 201 in turn to mix with the soil, and the motor 103 is turned on, and the motor 103 drives the extrusion block 107 to rotate through the rotating shaft 104. When the extrusion block 107 rotates in the first receiving chamber 101, the soil and the solution are preliminarily mixed, and when the extrusion block 107 rotates to the flexible protrusion 105, the extrusion block 107 squeezes the flexible protrusion 105 to make the flexible protrusion 105 concave, and the flexible protrusion 105 moves toward the high-pressure chamber 102, so that the air pressure in the high-pressure chamber 102 increases, and the deformed flexible protrusion 105 makes the volume in the first accommodating chamber 101 relatively increase. At this time, the liquid in the accommodating bottle 2 flows into the first accommodating chamber 101 and mixes with the soil mixed liquid in the first accommodating chamber 101. When the extrusion block 107 moves away from the flexible protrusion 105, the flexible protrusion 105 is reset under the action of the high-pressure chamber 102. At this time, the volume in the first accommodating chamber 101 becomes relatively smaller, and part of the mixed liquid in the first accommodating chamber 101 is squeezed upward. The mixing effect of the soil and the solution is effectively improved by the up and down flow of the liquid. At the same time, the solution is axially stirred in conjunction with the flexible stirring wire 112, thereby effectively improving the mixing effect of the solution and the soil.

[0044] When the solution in the containing bottle 2 flows into the first containing chamber 101, the solution is blocked by the filter plate 201, so that the solution has a certain impact force when entering the first containing chamber 101, thereby effectively improving the mixing effect of the solution in the containing bottle 2 and the solution in the first containing chamber 101, making the soil and the solution mix more evenly. At the same time, through the reciprocating flow of the solution, on the one hand, the filter plate 201 can be prevented from being blocked, and on the other hand, the fluidity of the solution can be increased, thereby improving the mixing effect of the solution.

[0045] When the soil and the solution are evenly mixed, the base 1 with the containing bottle 2 is placed in a constant temperature box for darkroom cultivation. During the cultivation process, the motor 103 drives the rotating shaft 104 to continue to rotate, so that the solution in the containing bottle 2 is always in a mixed state, preventing the internal soil from settling during the cultivation process and affecting the cultivation effect.

[0046] When the mixed solution in the container 2 is cultured, the heating area of ​​the mixed solution can be increased by providing a small diameter heating tube 205, so that the solution can reach the optimal culture temperature more quickly, and the culture time can be reduced, thereby effectively reducing the time for detecting dehydrogenase in the soil.

[0047] Example 2: Reference Figure 1-Figure 7 , a soil dehydrogenase detection device, which is basically the same as Example 1, further, the flexible protrusion 105 is set to be spherical, the extrusion block 107 is symmetrically provided with an arc-shaped grinding surface 108, and the outer side of the flexible protrusion 105 is provided with a first grinding surface 106 matching the arc-shaped grinding surface 108.

[0048] A first grinding area 109 and a second grinding area 110 are disposed between the flexible protrusion 105 and the spherical flexible protrusion 105 .

[0049] When the rotating shaft 104 drives the extrusion block 107 to rotate until it just fits with the spherical flexible protrusion 105, the first grinding surface 106 and the arc-shaped grinding surface 108 have a first grinding area 109 and a second grinding area 110, and the agglomerated soil located in the first grinding area 109 and the second grinding area 110 is ground, thereby effectively preventing the soil from being completely mixed with the detection solution and affecting the accuracy of soil dehydrogenase detection.

[0050] Example 3: Reference Figure 1-Figure 7 , a soil dehydrogenase detection device, is basically the same as Example 1, and further, an inclined plate 111 is symmetrically fixedly connected to the rotating shaft 104, and the inclined plate 111 is in contact with the bottom of the first accommodating cavity 101, and the inclined plate 111 and the extrusion block 107 are staggered.

[0051] The soil at the bottom of the first accommodating chamber 101 flows upward along the rotating inclined plate 111, and then contacts with the flexible protrusion 105 through the first extrusion block 107 for grinding, thereby effectively preventing the soil from accumulating and settling at the bottom of the first accommodating chamber 101. At the same time, the cooperation of the inclined plate 111 can effectively improve the grinding efficiency of the soil, thereby effectively improving the mixing effect of the solution and the soil.

[0052] A tapered surface 207 is provided between the temperature receiving pipe 205 and the connecting pipe 206 .

[0053] The conical surface 207 is provided to facilitate the downward flow of impurities at the connection between the connecting pipe 206 and the heated pipe 205 .

[0054] After the TTC solution and the glucose solution are added into the containing bottle 2 , the liquid levels are located at the connecting tube 206 .

[0055] After the TTC solution and the glucose solution are added, the liquid levels are located at the connecting tube 206, and when the liquid level in the containing bottle 2 rises or falls, the liquid level is always located at the connecting tube 206. Since the diameter of the connecting tube 206 is larger than the diameter of the heated tube 205, the relative height of the internal solution when it rises or falls is lower, and the area through which the liquid level moves when it changes in the connecting tube 206 is smaller, thereby effectively preventing excessive soil from adhering to the inner wall of the connecting tube 206 and affecting the accuracy of the dehydrogenase detection.

[0056] A sampling area 208 is provided on the connecting tube 206 .

[0057] By providing the sampling area 208 at the connecting pipe 206, the solution can be stored in the sampling area 208 and then sampled by the sampler.

[0058] Example 4: Reference Figure 1-Figure 7 A soil dehydrogenase detection device is basically the same as Example 1, and further, a plurality of first filter holes 202 matching the heated tube 205 are provided on the filter plate 201, an arc surface 204 is provided in the receiving bottle 2, and a plurality of second filter holes 203 matching the arc surface 204 are provided on the filter plate 201.

[0059] When the liquid in the first containing chamber 101 flows into the containing bottle 2, part of the liquid is sprayed upward along the first filter hole 202, causing turbulence in most of the liquid in the heated tube 205. The liquid sprayed from the second filter hole 203 contacts the curved surface 204, causing turbulence in the liquid between the heated tube 205 and the filter plate 201, thereby effectively preventing the generation of mixing dead corners in the containing bottle 2 that affect the mixing effect of the soil and the solution, thereby effectively improving the efficiency of the detection of dehydrogenase in the soil.

[0060] Example 5: A soil dehydrogenase detection method, mainly comprising the following steps:

[0061] Step 1: prepare reagents TTC, trimethylaminomethane, methanol and TPF, and configure them into a certain concentration;

[0062] Step 2: Screen the fresh soil, and add the quantitatively screened soil into the first containing chamber 101;

[0063] Step 3, thread the container 2 onto the base 1, then add appropriate amounts of TTC solution and glucose solution into the container 2, take another base 1 and container 2, add soil into the first container cavity 101 in the same manner, and then use trimethylaminomethane instead of TTC solution as a control;

[0064] Step 4: placing the container bottle 2 in a dark room of a constant temperature box for incubation for a certain period of time;

[0065] Step 5, turn on the motor 103, the motor 103 drives the extrusion block 107 through the rotating shaft 104 to rotate and squeeze the flexible protrusion 105 to increase the volume of the first accommodating chamber 101, and the internal liquid flows upward. When the extrusion block 107 moves away from the flexible protrusion 105, the air pressure in the high-pressure chamber 102 squeezes the flexible protrusion 105 to reset, so that the volume of the first accommodating chamber 101 becomes smaller, and the liquid in the accommodating bottle 2 flows downward;

[0066] Step 6: The flexible stirring wire 112 is driven by the rotating shaft 104 to stir the liquid so that the liquid is always in a mixed state;

[0067] Step 7: After the incubation is completed, add an appropriate amount of methanol to the container bottle 2 in small amounts and multiple times, and place the container bottle 2 on an oscillator to oscillate for a certain period of time, and then filter the liquid with filter paper. When filtering, first use a sampler to take out most of the liquid in the upper part, filter it with filter paper, and then replace the filter paper to pour out the remaining solution in the container bottle 2;

[0068] Step 8, measuring the volume of the filtrate, and placing the filtrate in a spectrophotometer to measure the absorbance at 485 nm;

[0069] Step 9. Calculate the dehydrogenase activity by comparing with the standard curve.

[0070] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0071] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A soil dehydrogenase detection device, comprising: The base is characterized by further comprising: A first accommodating cavity, disposed in the base; A rotating shaft, rotatably disposed in the first accommodating chamber; A motor is fixedly connected to the lower end of the base, and an output end of the motor is fixedly connected to the rotating shaft; A high-pressure chamber is arranged in the base, and the high-pressure chamber is coaxially arranged with the first accommodating chamber, a flexible protrusion is symmetrically arranged between the high-pressure chamber and the first accommodating chamber, and the flexible protrusion is fixedly connected to the base; An extrusion block is symmetrically fixedly connected to the rotating shaft, and the extrusion block is used to extrude the flexible protrusion; A receiving bottle, threaded and sealingly connected to the upper side of the base; A temperature receiving tube is arranged on the upper side of the containing bottle, and the diameter of the temperature receiving tube is smaller than the diameter of the containing bottle; A connecting pipe, fixedly connected to the upper side of the heated pipe, wherein the diameter of the connecting pipe is larger than the diameter of the heated pipe; The flexible stirring wire is fixedly arranged on the rotating shaft, and the flexible stirring wire is arranged in the heated tube.

2. A soil dehydrogenase detection device according to claim 1, characterized in that: The flexible protrusion is set to be spherical, the extrusion block is symmetrically provided with arc-shaped grinding surfaces, and the outer side of the flexible protrusion is provided with a first grinding surface matching the arc-shaped grinding surface.

3. A soil dehydrogenase detection device according to claim 2, characterized in that: A first grinding area and a second grinding area are arranged between the flexible protrusion and the spherical flexible protrusion.

4. A soil dehydrogenase detection device according to claim 2, characterized in that: The rotating shaft is symmetrically and fixedly connected with an inclined plate, and the inclined plate is in contact with the bottom of the first accommodating cavity, and the inclined plate and the extrusion block are arranged in a staggered manner.

5. A soil dehydrogenase detection device according to claim 1, characterized in that: A tapered surface is arranged between the temperature receiving pipe and the connecting pipe.

6. A soil dehydrogenase detection device according to claim 1, characterized in that: After the TTC solution and the glucose solution are added into the containing bottle, the liquid levels are located at the connecting tube.

7. A soil dehydrogenase detection device according to claim 1, characterized in that: A sampling area is arranged on the connecting pipe.

8. A soil dehydrogenase detection device according to claim 2, characterized in that: A filter plate sealed with the first accommodating cavity is arranged on the lower side of the accommodating bottle.

9. A soil dehydrogenase detection device according to claim 8, characterized in that: The filter plate is provided with a plurality of first filter holes matching the temperature receiving tubes, the receiving bottle is provided with an arc surface, and the filter plate is provided with a plurality of second filter holes matching the arc surface.

10. A soil dehydrogenase detection method, using the soil dehydrogenase detection device according to claim 1, characterized in that: The main steps include: Step 1: prepare reagents TTC, trimethylaminomethane, methanol and TPF, and configure them into a certain concentration; Step 2: Screening the fresh soil, and adding the quantitatively screened soil into the first containing chamber; Step 3: Connect the container bottle to the base with threads, then add appropriate amounts of TTC solution and glucose solution into the container bottle, take another base and container bottle, add soil into the first container chamber in the same manner, and then use trimethylaminomethane instead of TTC solution as a control; Step 4: Place the container bottle in a dark room of a constant temperature box and culture for a certain period of time; Step 5, turn on the motor, the motor drives the extrusion block to rotate through the rotating shaft to squeeze the flexible protrusion so that the volume in the first accommodating chamber becomes larger, and the internal liquid flows upward. When the extrusion block moves away from the flexible protrusion, the air pressure in the high-pressure chamber squeezes the flexible protrusion to reset, so that the volume in the first accommodating chamber becomes smaller, and the liquid in the accommodating bottle flows downward; Step 6: The flexible stirring wire is driven by the rotating shaft to stir the liquid so that the liquid is always in a mixed state; Step 7: After the culture is completed, add an appropriate amount of methanol to the container bottle in small amounts and multiple times, place the container bottle on an oscillator and oscillate for a certain period of time, and then filter the liquid with filter paper; Step 8, measuring the volume of the filtrate, and placing the filtrate in a spectrophotometer to measure the absorbance at 485 nm; Step 9. Calculate the dehydrogenase activity by comparing with the standard curve.

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