Magnetic stirring device and bioreactor

Abstract

The utility model provides a magnetic stirring device and a bioreactor, and the magnetic stirring device comprises a stirring shaft and a driving assembly for driving the stirring shaft to rotate, the driving assembly comprises a driving motor, an outer magnetic steel piece connected with the driving motor, an inner magnetic steel piece connected with the outer magnetic steel piece and the stirring shaft, a sealing head cover plate arranged at one end, close to the inner magnetic steel piece, of the stirring shaft in a sleeving manner, and a lip piece type sealing piece arranged on the stirring shaft in a sleeving manner; the lip piece type sealing piece is arranged on the side, away from the inner magnetic steel piece, of the end socket cover plate and abuts against the end socket cover plate. According to the bioreactor and the magnetic stirring device disclosed by the utility model, the lip piece type sealing piece is arranged, so that a culture solution can be prevented from entering the inner magnetic steel piece, frequent disassembly and cleaning are avoided, and the inner magnetic steel piece is prevented from being polluted.

Description

Technical Field

[0001] The utility model relates to a magnetic stirring device and a bioreactor. Background Art

[0002] Bioreactors are the main driving force for the industrialization of biopharmaceuticals. Whether it is microbial culture or cell culture, providing effective mass transfer for bioreactors is a necessary condition to ensure the metabolic growth of microorganisms or cells. A good stirring system is a key factor in improving the efficient mass transfer of bioreactors. There are currently two main mechanical transmission modes for bioreactor stirring systems. One is mechanical stirring in which the motor is directly connected to the stirring shaft, and the other is magnetic stirring in which the shaft is separated from the motor. Mechanical stirring is currently widely used due to its high-intensity driving force, but there are often problems such as leakage of stirring seals and locking of stirring shafts, resulting in failure of biological culture; while the magnetic stirring system has a good sealing effect, avoiding pollution caused by leakage of shaft seals and stirring systems, and is increasingly widely used in microbial and cell culture. However, the existing magnetic stirring system is easily infiltrated by culture fluid, resulting in the need for frequent cleaning.

[0003] In view of this, it is necessary to improve the existing magnetic stirring device to solve the above problems. Summary of the invention

[0004] The utility model aims to provide a magnetic stirring device to solve the problem that the existing magnetic stirring system is easily infiltrated by culture fluid.

[0005] To achieve the above-mentioned purpose, the utility model provides a magnetic stirring device, which includes a stirring shaft and a driving component for driving the stirring shaft to rotate. The driving component includes a driving motor, an external magnetic steel part connected to the driving motor, an internal magnetic steel part connecting the external magnetic steel part and the stirring shaft, a head cover plate sleeved on one end of the stirring shaft close to the internal magnetic steel part, and a lip seal sleeved on the stirring shaft, wherein the lip seal is arranged on a side of the head cover plate away from the internal magnetic steel part and abuts against the head cover plate.

[0006] As a further improvement of the present invention, the magnetic stirring device further comprises a locking nut for locking the lip seal on the head cover plate.

[0007] As a further improvement of the utility model, the stirring shaft is provided with a defoaming slurry and a stirring paddle, the defoaming slurry is arranged between the stirring paddle and the head cover plate, and the defoaming slurry is serrated.

[0008] As a further improvement of the present invention, the number of the stirring paddles is three and they are arranged at intervals along the height direction, and each of the stirring paddles includes two radial impellers and one axial impeller.

[0009] As a further improvement of the present invention, the external magnetic steel part includes an external magnetic steel hub close to the driving motor, an external magnetic steel inner cylinder section arranged in the middle and extending axially, a plurality of external magnetic poles arranged around the external magnetic steel inner cylinder section, and an external magnetic steel sealing plate arranged on the external magnetic pole near one end of the driving motor, the plurality of external magnetic poles include S poles and N poles alternately arranged along the circumferential direction, and the upper end of one type of external magnetic pole has a higher height in the vertical direction than another type of external magnetic pole.

[0010] As a further improvement of the present invention, the internal magnetic steel part includes an internal magnetic steel upper cover plate, an internal magnetic steel inner cylinder section arranged in the middle and extending axially, a plurality of internal magnetic poles arranged around the internal magnetic steel inner cylinder section, and an internal magnetic steel lower cover plate arranged at one end of the internal magnetic pole away from the internal magnetic steel upper cover plate, the plurality of internal magnetic poles include S poles and N poles alternately arranged along the circumferential direction, and the height of the lower end of one of the internal magnetic poles along the vertical direction is lower than that of another internal magnetic pole.

[0011] As a further improvement of the present invention, the internal magnetic steel part also includes an elastic component arranged on the internal magnetic steel lower sealing plate, and the elastic component includes an upper bearing abutting the internal magnetic steel lower sealing plate, an upper spring retaining ring connected below the upper bearing, a lower spring retaining ring arranged below the upper spring retaining ring, a lower bearing connected to the lower end of the lower spring retaining ring, and a spring connected between the upper spring retaining ring and the lower spring retaining ring.

[0012] As a further improvement of the utility model, the magnetic stirring device also includes a motor bracket for supporting the driving motor, the motor bracket is provided with a heat dissipation hole, and one end of the motor bracket facing the head cover plate is provided with a U-shaped groove.

[0013] As a further improvement of the utility model, the magnetic stirring device also includes an upper isolation sleeve and a lower isolation sleeve respectively sleeved on the outside of the inner magnetic steel part and the outside of the elastic component, the upper isolation sleeve and the lower isolation sleeve are fixed by threaded connection, the upper isolation sleeve and the lower isolation sleeve are accommodated in the motor bracket, and the lower isolation sleeve is provided with a boss for clamping with the U-shaped slot.

[0014] The utility model also provides a bioreactor, which comprises a reaction tank and the magnetic stirring device as described above, wherein the stirring shaft protrudes into the reaction tank, and the head cover plate seals the tank opening of the reaction tank.

[0015] The beneficial effect of the utility model is that the bioreactor and magnetic stirring device of the utility model can prevent the culture solution from entering the interior of the internal magnetic steel parts by setting the lip-type sealing parts, avoid frequent disassembly and cleaning, and prevent the internal magnetic steel parts from being contaminated. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the cross-sectional structure of the magnetic stirring device of the utility model;

[0017] Figure 2 It is a structural schematic diagram of a stirring shaft of a magnetic stirring device of the utility model;

[0018] Figure 3 It is a structural schematic diagram of a driving motor and a motor bracket of a magnetic stirring device of the utility model;

[0019] Figure 4 It is a structural schematic diagram of the outer magnetic steel part of the magnetic stirring device of the utility model;

[0020] Figure 5 It is a structural schematic diagram of the inner magnetic steel part of the magnetic stirring device of the utility model;

[0021] Figure 6 It is a structural schematic diagram of an upper isolation sleeve, a lower isolation sleeve and a locking nut of a magnetic stirring device of the utility model;

[0022] Figure 7 It is a schematic diagram of the exploded structure of the upper isolation sleeve and the lower isolation sleeve of the magnetic stirring device of the utility model;

[0023] Figure 8 The utility model is a schematic structural diagram of a locking nut and a lip seal of a magnetic stirring device. DETAILED DESCRIPTION

[0024] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0026] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. In addition, the technical features involved in the different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0027] like Figures 1 to 8 As shown, the bioreactor of the present invention includes a reaction tank and a magnetic stirring device 100 .

[0028] The magnetic stirring device 100 includes a stirring shaft 1 , a driving assembly 2 for driving the stirring shaft 1 to rotate, a locking nut 3 , a motor bracket 4 , an upper isolation sleeve 5 and a lower isolation sleeve 6 .

[0029] like Figure 2 As shown, the stirring shaft 1 is provided with a defoaming slurry 11 and a stirring paddle 12, and the defoaming slurry 11 and the stirring paddle 12 are both fixed to the stirring shaft 1 by screws.

[0030] The defoaming slurry 11 is arranged above the stirring paddle 12. The defoaming slurry 11 is sawtooth-shaped and can effectively break up bubbles.

[0031] The number of the stirring paddles 12 is greater than two. In the present embodiment, the number of the stirring paddles 12 is three and they are spaced apart in the height direction. Each of the stirring paddles 12 includes two radial impellers 121 and one axial impeller 122. The stirring paddles 12 have the effect of stirring the culture medium.

[0032] like Figure 1 , Figure 4 , Figure 5 and Figure 8 As shown, the driving assembly 2 includes a driving motor 21, an external magnetic steel part 22 connected to the driving motor 21, an internal magnetic steel part 23 connecting the external magnetic steel part 22 and the stirring shaft 1, a head cover plate 24 sleeved on one end of the stirring shaft 1 close to the internal magnetic steel part 23, and a lip seal 25 sleeved on the stirring shaft 1.

[0033] The motor bracket 4 is used to support the driving motor 21 .

[0034] The end cap plate 24 seals the tank opening of the reaction tank. The stirring shaft 1 passes through the end cap plate 24 and protrudes into the reaction tank. The defoaming slurry 11 is arranged between the stirring paddle 12 and the end cap plate 24.

[0035] like Figure 4 As shown, the external magnetic steel part 22 is arranged on the outside of the reaction tank, and the external magnetic steel part 22 includes an external magnetic steel hub 221 close to the driving motor 21, an external magnetic steel inner cylinder section 222 arranged in the middle and extending axially, a plurality of external magnetic poles 223 arranged around the external magnetic steel inner cylinder section 222, an external magnetic steel sealing plate 224 arranged at one end of the external magnetic pole 223 close to the driving motor 21, and an external magnetic steel outer cylinder section 225 arranged on the outside of the external magnetic pole 223. The plurality of external magnetic poles 223 include S poles and N poles alternately arranged along the circumferential direction, and the upper end of one type of external magnetic pole 223 has a higher height in the vertical direction than another type of external magnetic pole 223. The external magnetic poles 223 with different heights can transmit torque.

[0036] The outer magnetic steel member 22 is driven by the driving motor 21 to work.

[0037] like Figure 5 As shown, the internal magnetic steel part 23 includes an internal magnetic steel upper sealing plate 231, an internal magnetic steel inner cylinder section 232 arranged in the middle and extending axially, a plurality of internal magnetic poles 233 arranged around the internal magnetic steel inner cylinder section 232, an internal magnetic steel lower sealing plate 234 arranged at one end of the internal magnetic pole 233 away from the internal magnetic steel upper sealing plate 231, an elastic component 235 arranged on the internal magnetic steel lower sealing plate 234, and an internal magnetic steel outer cylinder section 236 arranged on the outside of the internal magnetic pole 233.

[0038] The plurality of inner magnetic poles 233 include S poles and N poles alternately arranged along the circumferential direction, and the lower end of one inner magnetic pole 233 has a lower height in the vertical direction than another inner magnetic pole 233. The inner magnetic poles 233 of different heights can transmit torque.

[0039] The elastic component 235 includes an upper bearing 2351 abutting against the inner magnetic steel lower sealing plate 234, an upper spring retaining ring 2352 connected to the bottom of the upper bearing 2351, a lower spring retaining ring 2353 arranged below the upper spring retaining ring 2352, a lower bearing 2354 connected to the lower end of the lower spring retaining ring 2353, and a spring 2355 connected between the upper spring retaining ring 2352 and the lower spring retaining ring 2353.

[0040] The elastic component 235 connects the stirring shaft 1 and the inner magnetic steel lower sealing plate 234 to drive the stirring shaft 1 to rotate.

[0041] The outer magnetic steel part 22 and the inner magnetic steel part 23 drive the stirring shaft 1 to rotate by means of upper and lower plane transmission, which can solve the problem of heat generation during high-speed transmission.

[0042] like Figure 3 As shown, the motor bracket 4 is provided with a heat dissipation hole 41 , and one end of the motor bracket 4 facing the head cover plate 24 is provided with a U-shaped slot 42 .

[0043] like Figure 6 to Figure 7 As shown, the upper isolation sleeve 5 and the lower isolation sleeve 6 are respectively sleeved on the outside of the inner magnetic steel part 23 and the elastic component 235, and the upper isolation sleeve 5 and the lower isolation sleeve 6 are fixed by threaded connection, which is convenient for disassembly, and an O-ring 7 is provided between the upper isolation sleeve 5 and the lower isolation sleeve 6 to play a sealing role.

[0044] The upper isolation sleeve 5 and the lower isolation sleeve 6 are accommodated in the motor bracket 4 , and the lower isolation sleeve 6 is provided with a boss for engaging with the U-shaped slot 42 to limit the position of the motor bracket 4 .

[0045] The upper isolation sleeve 5 is arranged in a flower arrangement style for easy disassembly. In addition, the space in the upper isolation sleeve 5 and the lower isolation sleeve 6 is a sterile environment.

[0046] like Figure 1 and Figure 8 As shown, the lip seal 25 is arranged on a side of the head cover plate 24 away from the inner magnetic steel part 23 and abuts against the head cover plate 24 .

[0047] The locking nut 3 is used to lock the lip seal 25 on the head cover plate 24 .

[0048] In this embodiment, the lip seal 25 and the locking nut 3 are arranged in the cavity of the reaction tank below the head cover plate 24. The lip seal 25 can isolate the inside and outside of the reaction tank, and cooperate with the locking nut 3 to limit and fix it, which can prevent the culture solution from entering the inner magnetic steel part 23 to cause dead corners and pollution, avoid frequent disassembly and cleaning, improve experimental efficiency and avoid aging, loosening and pollution of parts caused by frequent disassembly.

[0049] In addition, even if the lip seal 25 fails, since the inner cavities of the upper isolation sleeve 5 and the lower isolation sleeve 6 are sterile cavities, it will not cause the failure of the culture batch, effectively ensuring the smooth progress of the culture of this batch. The lip seal 25 needs to be replaced in time for the next batch, and the inner cavities of the upper isolation sleeve 5 and the lower isolation sleeve 6 need to be cleaned and sterilized.

[0050] The bioreactor and magnetic stirring device 100 of the present invention can prevent the culture fluid from entering the inner magnetic steel part 23 by providing the lip seal 25, thereby avoiding frequent disassembly and cleaning and preventing the inner magnetic steel part 23 from being contaminated.

[0051] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0052] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A magnetic stirring device, characterized in that: The magnetic stirring device includes a stirring shaft and a driving component for driving the stirring shaft to rotate. The driving component includes a driving motor, an external magnetic steel part connected to the driving motor, an internal magnetic steel part connecting the external magnetic steel part and the stirring shaft, a head cover plate sleeved on one end of the stirring shaft close to the internal magnetic steel part, and a lip seal sleeved on the stirring shaft. The lip seal is arranged on a side of the head cover plate away from the internal magnetic steel part and abuts against the head cover plate.

2. The magnetic stirring device according to claim 1, characterized in that: The magnetic stirring device also includes a locking nut for locking the lip seal on the head cover plate.

3. The magnetic stirring device according to claim 1, characterized in that: The stirring shaft is provided with a defoaming slurry and a stirring paddle, the defoaming slurry is arranged between the stirring paddle and the head cover plate, and the defoaming slurry is in a sawtooth shape.

4. The magnetic stirring device according to claim 3, characterized in that: The number of the stirring paddles is three and they are arranged at intervals along the height direction. Each of the stirring paddles includes two radial impellers and one axial impeller.

5. The magnetic stirring device according to claim 1, characterized in that: The external magnetic steel part includes an external magnetic steel hub close to the driving motor, an external magnetic steel inner cylinder section arranged in the middle and extending axially, a plurality of external magnetic poles arranged around the external magnetic steel inner cylinder section, and an external magnetic steel sealing plate arranged on one end of the external magnetic pole close to the driving motor, the plurality of external magnetic poles include S poles and N poles alternately arranged along the circumferential direction, and the upper end of one type of external magnetic pole has a higher height in the vertical direction than another type of external magnetic pole.

6. The magnetic stirring device according to claim 5, characterized in that: The internal magnetic steel part includes an internal magnetic steel upper cover plate, an internal magnetic steel inner cylinder section arranged in the middle and extending axially, a plurality of internal magnetic poles arranged around the internal magnetic steel inner cylinder section, and an internal magnetic steel lower cover plate arranged at one end of the internal magnetic pole away from the internal magnetic steel upper cover plate. The plurality of internal magnetic poles include S poles and N poles alternately arranged along the circumferential direction, and the height of the lower end of one type of internal magnetic pole along the vertical direction is lower than that of another type of internal magnetic pole.

7. The magnetic stirring device according to claim 6, characterized in that: The inner magnetic steel part also includes an elastic component arranged on the inner magnetic steel lower sealing plate, and the elastic component includes an upper bearing abutting the inner magnetic steel lower sealing plate, an upper spring retaining ring connected below the upper bearing, a lower spring retaining ring arranged below the upper spring retaining ring, a lower bearing connected to the lower end of the lower spring retaining ring, and a spring connected between the upper spring retaining ring and the lower spring retaining ring.

8. The magnetic stirring device according to claim 7, characterized in that: The magnetic stirring device also includes a motor bracket for supporting the driving motor, the motor bracket is provided with a heat dissipation hole, and one end of the motor bracket facing the head cover plate is provided with a U-shaped groove.

9. The magnetic stirring device according to claim 8, characterized in that: The magnetic stirring device also includes an upper isolation sleeve and a lower isolation sleeve respectively sleeved on the outside of the inner magnetic steel part and the outside of the elastic component, the upper isolation sleeve and the lower isolation sleeve are fixed by threaded connection, the upper isolation sleeve and the lower isolation sleeve are accommodated in the motor bracket, and the lower isolation sleeve is provided with a boss for clamping with the U-shaped slot.

10. A bioreactor, characterized in that: The bioreactor comprises a reaction tank and the magnetic stirring device as described in any one of claims 1 to 9, the stirring shaft protrudes into the reaction tank, and the head cover plate seals the tank mouth of the reaction tank.

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