Automatic cell culture device
By using detachable gears and driving motors to drive the culture bottle to rotate in the automatic cell culture device, the slipping problem caused by roller wear is solved, and stable suspension culture and equipment life is achieved.
Patent Information
- Application Number
- CN202420683585.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-03
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2034-04-03
AI Technical Summary
In existing suspended cell culture devices, long-term contact between the roller and the culture bottle leads to wear of the anti-slip layer, causing slippage, the rotation speed of the culture bottle decreases, and the cells are prone to precipitation on the inner wall of the culture bottle, and cannot be effectively suspended and cultured.
Design an automatic cell culture device, using a detachable first rotating gear and meshing with the second rotating gear, and drive the culture bottle to rotate through the sliding plate and the driving motor, instead of roller rolling, the rotation of the culture bottle is realized, and the device and culture bottle are enhanced. Closely cooperate to extend service life.
The stable rotation of the culture flask is achieved, the friction is reduced, the service life of the equipment is extended, and the suspension culture effect of the cells is ensured.
Smart Images
Figure CN222139140U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture devices, and in particular to an automatic cell culture device. Background Art
[0002] Cell suspension culture is a cell culture method performed in liquid culture medium. Unlike traditional adherent cell culture, cells in suspension culture do not adhere to solid or semi-solid surfaces, but float freely in the culture medium. This method is mainly used for cells that can grow in liquid media, such as blood cells, certain cancer cells, and genetically modified cells.
[0003] The working process of the existing suspension cell culture device is: first, the cells to be cultured are placed in a culture bottle, and then the culture bottle is placed in the culture device, and the culture bottle is driven to rotate by the rotation of the roller at the bottom of the culture device, so that the cells in the culture bottle can float freely in the culture bottle and will not sink to the bottom wall of the culture bottle.
[0004] Although this can achieve suspension culture of cells, there are still the following disadvantages: if the outer surface of the culture bottle is in contact with the roller for a long time, the anti-skid layer on the roller may be worn and consumed, causing slippage between the roller and the culture bottle, reducing the rotation speed of the culture bottle, and the cells in the culture bottle are easily precipitated on the inner wall of the culture bottle, making it impossible for some cells to be suspended and cultured. Utility Model Content
[0005] The utility model provides an automatic cell culture device, which solves the problem in the prior art that envelopes are easily separated from a conveyor belt when a packaging machine sprays glue, thereby improving the practicability of the device.
[0006] The technical solution of the utility model is as follows:
[0007] A cell automatic culture device comprises a bottom box and a culture bottle.
[0008] A first rotating gear is detachably connected to the outer wall of the culture bottle, a limiting structure for placing the culture bottle and the first rotating gear is provided on the bottom box, a top plate is provided on the top of the bottom box, a plurality of sliding columns are connected and fixed between the top plate and the bottom box, a sliding plate is provided between the top plate and the bottom box, each of the sliding columns is slidably connected to the sliding plate, a second rotating gear is provided on the sliding plate, the second rotating gear is meshed with the first rotating gear, and a driving structure for driving the second rotating gear to rotate is also provided on the sliding plate.
[0009] Furthermore, the outer surface of the culture bottle is a cylindrical structure, the end of the culture bottle is provided with a liquid inlet, the diameter of the liquid inlet is smaller than the diameter of the culture bottle, the outer wall of the culture bottle is provided with a first card groove, the cross-section of the first card groove is a circular ring structure, and a card is also fixed in the first card groove.
[0010] Furthermore, the first rotating gear includes a first connecting member and a second connecting member, the outer surface of the first connecting member is a semi-gear type structure, the first connecting member is provided with an extension plate close to the second connecting member side, the first connecting member is also provided with a mounting hole that is engaged with the first slot, the center of the mounting hole coincides with the center of the first connecting member, the mounting hole is also provided with a second slot that is engaged with the slot, the first connecting member and the second connecting member have the same structure, and the extension plate of the first connecting member is fixed to the extension plate of the second connecting member by bolts.
[0011] Furthermore, the limiting structure includes a first placement groove, the shape of the first placement groove is the same as the shape of the outer surface of the culture bottle, a second placement groove is arranged in the first placement groove, the vertical projection surface of the second rotating gear falls in the second placement groove, and a third rotating gear is also rotatably connected to the inner wall of the second placement groove.
[0012] Furthermore, the vertical projection surfaces of the top plate and the bottom box are both rectangular structures, the number of the sliding columns is four and they are respectively placed near the four corner ends of the top plate, and the projection surface of the sliding columns is a circular structure.
[0013] Furthermore, a sliding hole slidably connected to the sliding column is provided on the sliding plate, a telescopic member is provided between the sliding plate and the top plate, a fixed end of the telescopic member is fixedly connected to the top plate, and a telescopic end of the telescopic member is fixedly connected to the sliding plate.
[0014] Furthermore, a mounting frame is provided at the bottom of the sliding plate, and the cross-section of the mounting frame has an "L"-shaped structure. The mounting frame includes a mounting plate, a connecting plate is fixedly connected between the mounting plate and the sliding plate, a driving motor is fixed on the mounting plate, and the output shaft of the driving motor is fixedly connected to the second rotating gear.
[0015] The working principle and beneficial effects of the utility model are:
[0016] The working process of this embodiment is as follows: first, the cells to be cultured and the culture fluid are loaded into the culture bottle, and then the first connecting piece and the second connecting piece are simultaneously inserted into the slots on the culture bottle, and the first connecting piece and the second connecting piece are connected and fixed by bolts, and then the culture bottle is placed in the first placement slot on the bottom box, so that the first transmission gear is meshed with the third rotating gear. At this time, the telescopic piece is started to bring the second rotating gear close to the first transmission gear of the inner core, and finally the driving motor is started to drive the culture bottle to rotate to realize suspension culture.
[0017] In this embodiment, a first rotating gear is arranged on the outer wall of the culture bottle, and a second rotating gear is arranged on the bottom box. The second rotating gear is driven by the sliding plate to approach or move away from the first rotating gear to engage with the first rotating gear, so as to complete the installation and removal of the culture bottle on the bottom box, so that the culture bottle can be rotated by gear transmission, instead of the prior art that relies on the rolling of the roller to drive the culture bottle to rotate. The utility model has a tighter fit with the culture bottle and a longer service life. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] The utility model is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.
[0019] Figure 1 It is a structural schematic diagram of the prior art of this embodiment;
[0020] Figure 2 The overall structure of this embodiment is shown in FIG. Figure 1 ;
[0021] Figure 3 The overall structure of this embodiment is shown in FIG. Figure 2 ;
[0022] Figure 4 Schematic diagram of the internal structure of the bottom box in this embodiment;
[0023] Figure 5 Schematic diagram of the internal structure of the first rotating gear in this embodiment.
[0024] In the figure:
[0025] 1. Culture bottle; 11. Liquid inlet; 12. First card slot; 121. Card member; 2. First rotating gear; 21. First connecting member; 211. Extension plate; 212. Mounting hole; 2121. Second card slot; 22. Second connecting member; 3. Bottom box; 31. Sliding column; 32. First placement slot; 33. Second placement slot; 4. Second rotating gear; 5. Top plate; 51. Telescopic member; 6. Sliding plate; 61. Mounting plate; 62. Connecting plate; 63. Driving motor; 64. Sliding hole; 7. Third rotating gear. DETAILED DESCRIPTION
[0026] The following will be combined with the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0027] like Figure 2 to Figure 5As shown, a cell automatic culture device, the structure of which includes a bottom box 3 and a culture bottle 1. The first rotating gear 2 in this embodiment is detachably connected to the outer wall of the culture bottle 1. A limiting structure is arranged on the bottom box 3, for placing the culture bottle 1 and the first rotating gear 2. A top plate 5 is arranged on the top of the bottom box 3, and a plurality of sliding columns 31 are fixedly arranged between the top plate 5 and the bottom box 3. A sliding plate 6 is arranged between the top plate 5 and the bottom box 3, and each sliding column 31 is slidably connected to the sliding plate 6. The second rotating gear 4 is arranged on the sliding plate 6, and the second rotating gear 4 is meshed with the first rotating gear 2. A driving structure is arranged on the sliding plate 6, for driving the second rotating gear 4 to rotate.
[0028] The limiting structure in this embodiment includes a first placement groove 32, the shape of the first placement groove 32 is the same as the shape of the outer surface of the culture bottle 1, and is used to limit the culture bottle 1 so that the culture bottle 1 will not be separated from or deviated from the first placement groove 32 when rotating. The second placement groove 33 is arranged in the first placement groove 32, the vertical projection surface of the second rotating gear 4 falls in the second placement groove 33, and the third rotating gear 7 is rotatably arranged on the inner wall of the second placement groove 33. The third rotating gear 7 in this embodiment is designed to reduce the contact between the culture bottle 1 and the first placement groove 32 and the second placement groove 33, so that the friction of the culture bottle 1 during the rotation process is reduced, and the service life of the culture bottle 1 is extended.
[0029] In this embodiment, the vertical projection surfaces of the top plate 5 and the bottom box 3 are both rectangular structures, and there are four sliding columns 31, which are respectively placed near the four corner ends of the top plate 5, and the projection surface of the sliding columns 31 is a circular structure. The design of the four sliding columns 31 ensures that the sliding plate 6 will not be offset when sliding on the sliding columns 31, and the sliding of the sliding plate 6 is more stable through multi-angle positioning.
[0030] The sliding hole 64 in this embodiment is arranged on the sliding plate 6, and it is slidingly connected with the sliding column 31. The telescopic member 51 is arranged between the sliding plate 6 and the top plate 5, the fixed end of the telescopic member 51 is fixedly connected with the top plate 5, and the telescopic end of the telescopic member 51 is fixedly connected with the sliding plate 6. The telescopic member 51 in this embodiment is preferably a hydraulic cylinder or a pneumatic cylinder. The hydraulic cylinder or the cylinder in this embodiment are all prior art, and will not be described in detail in this embodiment. The sliding of the sliding plate 6 is controlled by the hydraulic cylinder or the cylinder, so that a certain buffering force is generated when the first rotating gear 2 contacts the second rotating gear 4, thereby reducing internal mechanical collision damage.
[0031] The bottom of the sliding plate 6 in this embodiment is provided with a mounting frame, the cross section of the mounting frame is an "L"-shaped structure, the mounting frame includes a mounting plate 61, a connecting plate 62 is fixedly arranged between the mounting plate 61 and the sliding plate 6, a driving motor 63 is fixedly arranged on the mounting plate 61, and the output shaft of the driving motor 63 is fixedly connected to the second rotating gear 4. This is the design of the installation of the driving motor 63, so that the first rotating gear 2 can operate stably without jamming or deviation.
[0032] The outer surface of the culture bottle 1 in this embodiment is a cylindrical structure, which is used to facilitate the rotation of the culture bottle 1 on the culture device. The liquid inlet 11 is set at the end of the culture bottle 1. The diameter of the liquid inlet 11 is smaller than the diameter of the culture bottle 1, which is used to make the cells gather in the culture bottle 1, reduce the number of cells at the bottle mouth, and prevent cell leakage. The first card slot 12 is set on the outer wall of the culture bottle 1. The cross-section of the first card slot 12 is a circular ring structure. The clamp 121 is fixedly set in the first card slot 12 to facilitate the installation of the first rotating gear 2. This design is to ensure that when the first rotating gear 2 rotates, the culture bottle 1 will rotate along with the first rotating gear 2.
[0033] The first rotating gear 2 in this embodiment includes a first connecting member 21 and a second connecting member 22. The outer surface of the first connecting member 21 is a semi-gear-shaped structure. The extension plate 211 is arranged on the side of the first connecting member 21 close to the second connecting member 22. The mounting hole 212 is arranged on the first connecting member 21, and it is engaged with the first clamping groove 12. The center of the mounting hole 212 coincides with the center of the first connecting member 21. The second clamping groove 2121 is arranged on the mounting hole 212, and it is engaged with the clamping member 121. The first connecting member 21 and the second connecting member 22 have the same structure. The extension plate 211 of the first connecting member 21 and the extension plate 211 of the second connecting member 22 are fixed by bolts. This design is to facilitate the disassembly and installation of the first rotating gear 2 relative to the culture bottle 1, so that the culture bottle 1 is easier to clean.
[0034] The working process of this embodiment is as follows: first, the cells to be cultured and the culture fluid are loaded into the culture bottle 1, and then the first connecting member 21 and the second connecting member 22 are simultaneously inserted into the slots on the culture bottle 1, and the first connecting member 21 and the second connecting member 22 are connected and fixed by bolts, and then the culture bottle 1 is placed in the first placement slot 32 on the bottom box 3, so that the first transmission gear is meshed with the third rotating gear 7, and at this time, the telescopic member 51 is started to bring the second rotating gear 4 close to the first transmission gear of the inner core, and finally the driving motor 63 is started to drive the culture bottle 1 to rotate to realize suspension culture.
[0035] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. An automatic cell culture device, comprising a bottom box (3) and a culture bottle (1), characterized in that: A first rotating gear (2) is detachably connected to the outer wall of the culture bottle (1); a limiting structure for placing the culture bottle (1) and the first rotating gear (2) is provided on the bottom box (3); a top plate (5) is provided on the top of the bottom box (3); a plurality of sliding columns (31) are connected and fixed between the top plate (5) and the bottom box (3); a sliding plate (6) is provided between the top plate (5) and the bottom box (3); each of the sliding columns (31) is slidingly connected to the sliding plate (6); a second rotating gear (4) is provided on the sliding plate (6); the second rotating gear (4) is meshed with the first rotating gear (2); and a driving structure for driving the second rotating gear (4) to rotate is also provided on the sliding plate (6).
2. The automatic cell culture device according to claim 1, characterized in that: The outer surface of the culture bottle (1) is a cylindrical structure. The end of the culture bottle (1) is provided with a liquid inlet (11). The diameter of the liquid inlet (11) is smaller than the diameter of the culture bottle (1). The outer wall of the culture bottle (1) is provided with a first clamping groove (12). The cross section of the first clamping groove (12) is a circular ring structure. A clamping piece (121) is also fixed in the first clamping groove (12).
3. The automatic cell culture device according to claim 2, characterized in that: The first rotating gear (2) comprises a first connecting member (21) and a second connecting member (22); the outer surface of the first connecting member (21) is a semi-gear-shaped structure; the first connecting member (21) is provided with a protruding plate (211) on the side close to the second connecting member (22); the first connecting member (21) is also provided with a mounting hole (212) engaged with the first clamping groove (12); the center of the mounting hole (212) coincides with the center of the first connecting member (21); the mounting hole (212) is also provided with a second clamping groove (2121) engaged with the clamping member (121); the first connecting member (21) and the second connecting member (22) have the same structure; the protruding plate (211) of the first connecting member (21) and the protruding plate (211) of the second connecting member (22) are fixed by bolt connection.
4. The automatic cell culture device according to claim 1, characterized in that: The limiting structure comprises a first placement groove (32), the shape of the first placement groove (32) is the same as the shape of the outer surface of the culture bottle (1), a second placement groove (33) is arranged inside the first placement groove (32), the vertical projection surface of the second rotating gear (4) falls inside the second placement groove (33), and a third rotating gear (7) is also rotatably connected to the inner wall of the second placement groove (33).
5. The automatic cell culture device according to claim 4, characterized in that: The projection surfaces of the top plate (5) and the bottom box (3) in the vertical direction are both rectangular structures. The number of the sliding columns (31) is four and they are respectively arranged at four corner ends close to the top plate (5). The projection surface of the sliding columns (31) is a circular structure.
6. The automatic cell culture device according to claim 5, characterized in that: The sliding plate (6) is provided with a sliding hole (64) that is slidingly connected to the sliding column (31); a telescopic member (51) is provided between the sliding plate (6) and the top plate (5); the fixed end of the telescopic member (51) is fixedly connected to the top plate (5); and the telescopic end of the telescopic member (51) is fixedly connected to the sliding plate (6).
7. The automatic cell culture device according to claim 6, characterized in that: A mounting frame is provided at the bottom of the sliding plate (6), and the cross-section of the mounting frame is an "L"-shaped structure. The mounting frame comprises a mounting plate (61), a connecting plate (62) is fixedly connected between the mounting plate (61) and the sliding plate (6), a driving motor (63) is fixed on the mounting plate (61), and an output shaft of the driving motor (63) is fixedly connected to the second rotating gear (4).